CN114886818A

CN114886818A - Anti-dandruff composition, shampoo and preparation method thereof

Info

Publication number: CN114886818A
Application number: CN202210657698.9A
Authority: CN
Inventors: 徐伟超; 梁嘉明; 丛琳; 李雪竹; 刘亚军
Original assignee: Anfu Chaowei Rihua Co ltd
Current assignee: Anfu Chaowei Rihua Co ltd
Priority date: 2022-06-10
Filing date: 2022-06-10
Publication date: 2022-08-12

Abstract

The invention provides an anti-dandruff composition, shampoo and a preparation method thereof, wherein the anti-dandruff composition comprises a traditional Chinese medicine extract and a malassezia inhibitor in a predetermined mass ratio, and can inhibit the reproduction of malassezia and repair scalp and remove dandruff. The shampoo comprises a base formula and the anti-dandruff composition added in the base formula, wherein the base formula can provide a mild carrier for the anti-dandruff composition so as to achieve better anti-dandruff effect. The embodiment of the invention also provides a preparation method of the shampoo, and the preparation method is simple in process, low in cost and capable of realizing industrial production.

Description

Anti-dandruff composition, shampoo and preparation method thereof

Technical Field

The invention relates to the technical field of household bath products, and in particular relates to an anti-dandruff composition, shampoo and a preparation method thereof.

Background

Shampoo is one of common household bath products, and has the main functions of cleaning scalp and hair and keeping the scalp and the hair clean and sanitary. With the improvement of living standard, people have higher and higher requirements on the quality and the function of the shampoo, and the requirements are not satisfied with the cleaning efficacy of the shampoo alone.

The problem of dandruff has long plagued many people. Dandruff is the exfoliative corneocytes of the scalp. Normally, keratinocytes shed very little, slowly and are not seen significantly. However, under certain stimulation, a large amount of immature keratinocytes are produced, agglomerated and shed, and a phenomenon of 'snowflakes', namely dandruff, which is raised in a dispute is formed. During dandruff formation, two substances play an important role: oil and fat secreted from scalp, and malassezia that feeds on oil and fat. Under certain conditions, such as when the grease secretion in adolescence is vigorous, malassezia can propagate on the surface of the scalp in a large quantity to stimulate scalp cells to secrete more grease; a large amount of grease provides nutrients for further amplification of malassezia. Finally, the normal scalp stratum corneum cell metabolism becomes unstable, and immature keratinocytes aggregate and fall off in a row, and dandruff is generated.

Disclosure of Invention

In view of the above, the first object of the present invention is to provide an anti-dandruff composition capable of inhibiting the proliferation of malassezia.

It is a second object of the present invention to provide a shampoo having an anti-dandruff effect.

The third purpose of the invention is to provide a preparation method of shampoo, which has simple process and low cost and can be industrially produced.

In order to achieve the purpose, the invention provides the following technical scheme:

the invention firstly provides an anti-dandruff composition, which comprises traditional Chinese medicine extracts and a malassezia inhibitor in a predetermined mass ratio, wherein the traditional Chinese medicine extracts comprise a cacumen biotae extract, an angelica sinensis extract and a sophora flavescens extract.

Preferably, the mass ratio of the cacumen biotae extract to the angelica sinensis extract to the sophora flavescens extract to the malassezia inhibitor is (1-50): (1-50): (1-50): (10-50).

More preferably, the malassezia inhibitor is a pyrrolidone ethanolamine salt.

The present invention also provides a shampoo comprising a base formulation and an anti-dandruff composition as described above added to the base formulation.

Preferably, the base formulation comprises the following ingredients: deionized water, EDTA-2Na, polyquaternium-10, guar gum hydroxypropyl trimethyl ammonium chloride, citric acid, cocamidopropyl betaine, sodium laureth sulfate, ammonium laureth sulfate, cocamide MEA, ethylene glycol distearate, stearyl alcohol, alkyl glycoside, sodium lauroyl sarcosinate, sodium benzoate, polyquaternium-73, dimethiconol, phenoxyethanol, essence and maltotetraose.

More preferably, the shampoo comprises the following components in percentage by mass:

50 to 80 percent of deionized water;

0.05 to 0.1 percent of EDTA-2 Na;

0.05 to 0.2 percent of polyquaternium-10;

0.05 to 0.2 percent of guar hydroxypropyl trimethyl ammonium chloride;

0.05 to 0.2 percent of citric acid;

2-10% of cocamidopropyl betaine;

5 to 20 percent of lauryl alcohol polyether sodium sulfate;

1% -8% of ammonium lauryl sulfate;

1% -4% of coconut oil amide MEA;

1-3% of ethylene glycol distearate;

0.1 to 0.2 percent of octadecanol;

1-3% of alkyl glycoside;

1-4% of sodium lauroyl sarcosinate;

0.1 to 0.5 percent of sodium benzoate;

0.5% -2% of polyquaternium-73;

1 to 8 percent of dimethiconol;

0.3 to 0.6 percent of phenoxyethanol;

0.1 to 0.5 percent of essence;

0.5 to 1 percent of maltotetraose;

0.01 to 0.5 percent of biota orientalis leaf extract;

0.01 to 0.5 percent of angelica extract;

0.01 to 0.5 percent of sophora flavescens extract;

0.1% -0.5% of pyrrolidone ethanolamine salt.

Still preferably, in the shampoo, the mass fraction of the cacumen biotae extract is 0.08%, the mass fraction of the angelica sinensis extract is 0.16%, the mass fraction of the sophora flavescens extract is 0.32%, and the mass fraction of the pyrrolidone ethanolamine salt is 0.1%.

Still preferably, the shampoo contains 0.08% of the cacumen biotae extract, 0.16% of the angelica sinensis extract, 0.32% of the sophora flavescens extract and 0.2% of the pyrrolidone ethanolamine salt.

The invention also provides a preparation method of the shampoo, which comprises the following steps:

s100, dispersing the EDTA-2Na, the polyquaternium-10 and the guar gum propyl trimethyl ammonium chloride in deionized water to obtain a first solution;

s200, heating the first solution, adding the citric acid and the cocamidopropyl betaine to the first solution in sequence, then adding the sodium laureth sulfate, the ammonium laureth sulfate, the cocamide MEA, the ethylene glycol distearate, the stearyl alcohol, the alkyl glycoside and the sodium lauroyl sarcosinate to obtain a second solution, and keeping the temperature at 80-85 ℃;

s300, cooling the second solution to 60-65 ℃, and adding the polydimethylsiloxane alcohol and the polyquaternium-73 to obtain a third solution;

s400, cooling the third solution to 40-45 ℃, and adding the sodium benzoate, the phenoxyethanol, the essence, the maltotetraose, the cacumen biotae extract, the angelica sinensis extract, the radix sophorae flavescentis extract and the pyrrolidone ethanolamine salt to obtain the shampoo.

Preferably, in the step S200, the holding time of the second solution is 30min to 60 min.

The embodiment of the invention provides an anti-dandruff composition which comprises traditional Chinese medicine extracts and a malassezia inhibitor in a predetermined mass ratio, and can inhibit the propagation of malassezia and repair scalp. Embodiments of the present invention also provide a shampoo comprising a base formulation and the anti-dandruff composition added to the base formulation, wherein the base formulation can provide a mild vehicle for the anti-dandruff composition to achieve a better anti-dandruff effect. The embodiment of the invention also provides a preparation method of the shampoo, and the preparation method is simple in process, low in cost and capable of realizing industrial production.

Drawings

Fig. 1 is a process flow chart of a method for preparing a shampoo according to an embodiment of the present invention.

Detailed Description

For the purposes of the present invention; technical solutions and advantages will become more apparent, and a detailed description of embodiments of the present invention will be given below with reference to the accompanying drawings. Examples of these preferred embodiments are illustrated in the accompanying drawings. The embodiments of the invention shown in the drawings and described in accordance with the drawings are exemplary only, and the invention is not limited to these embodiments.

It should be noted that, in order to avoid obscuring the present invention with unnecessary details, only the structures and/or processing steps closely related to the scheme according to the present invention are shown in the drawings, and other details not so relevant to the present invention are omitted.

The inventors of the present invention have made the following drawbacks of shampoos of the prior art, and a first object of the present invention is to provide an anti-dandruff composition capable of inhibiting the proliferation of malassezia bacteria; a second object of the present invention is to provide a shampoo having an anti-dandruff effect; the third purpose of the invention is to provide a preparation method of shampoo, which has simple process and low cost and can be industrially produced.

It is worth mentioning that in the anti-dandruff composition provided in the embodiments of the present invention, the malassezia inhibitor can be consistent with the proliferation of malassezia and simultaneously has irritation, and the traditional Chinese medicine extract can reduce the irritation of the malassezia inhibitor and repair scalp, in the traditional Chinese medicine extract, the cacumen biotae extract contains flavonoid compounds, has an astringent effect, and can protect skin mucosa; the angelica sinensis extract can improve the microcirculation of the scalp, promote the scalp to absorb nutrition and provide rich trace elements for the hair; the radix Sophorae Flavescentis extract is a natural dandruff removing component, and has effects of killing parasite, relieving itching, and eliminating dampness and heat. The anti-dandruff composition provided by the embodiment of the invention comprises the traditional Chinese medicine extract and the malassezia inhibitor in a predetermined mass ratio, can inhibit the proliferation of the malassezia, can repair scalp, and has an anti-dandruff effect.

Specifically, in the anti-dandruff composition provided by the embodiment of the present invention, the mass ratio of the cacumen biotae extract, the angelica sinensis extract, the radix sophorae flavescentis extract and the malassezia inhibitor is (1-50): (1-50): (1-50): (10-50).

More specifically, the malassezia inhibitor is pyrrolidone ethanolamine salt.

It should be noted that although the anti-dandruff composition provided by the embodiments of the present invention has an excellent anti-dandruff effect, the anti-dandruff composition still has a certain irritation to the scalp, and if the shampoo provided by the embodiments of the present invention has insufficient mildness, the anti-dandruff composition directly irritates the scalp, so that the dandruff problem on the head is vicious. Thus, as a preferred option, the shampoo provided by the embodiments of the present invention preferably provides a mild base formulation that provides a mild carrier for the anti-dandruff composition, so as to achieve better anti-dandruff effect.

Specifically, the basic formula comprises the following components: deionized water, EDTA-2Na, polyquaternium-10, guar gum hydroxypropyl trimethyl ammonium chloride, citric acid, cocamidopropyl betaine, sodium laureth sulfate, ammonium laureth sulfate, cocamide MEA, ethylene glycol distearate, stearyl alcohol, alkyl glycoside, sodium lauroyl sarcosinate, sodium benzoate, polyquaternium-73, dimethiconol, phenoxyethanol, essence and maltotetraose.

In the basic formula, EDTA-2Na is an important complexing agent, compounding agent and detergent; the guar gum hydroxypropyl trimethyl ammonium chloride can be combined with the polyquaternium-10 to form a layer of water-soluble soft protective film, and the anti-static effect can be remarkable in a dry environment; the citric acid can keep the shampoo at a proper pH value, and the interaction of the acidic pH value and a trace amount of negative charges on the hair can help the hair skin to keep a smooth and flat surface; the cocoamidopropyl betaine and the sodium laureth sulfate are matched to play a synergistic effect, the experience feeling in the hair washing process can be enhanced, abundant and dense foams can bring excellent touch feeling and softness in the wet flushing process, and the high-density foam has solubility, hard water resistance, foamability and wetting power and still has good detergency in hard water. The ammonium lauryl sulfate and the coconut oil amide MEA are compounded, so that the foaming property of the shampoo can be improved, and the cleaning strength is enhanced; the ethylene glycol distearate can impart beautiful pearl effect to the shampoo; the octadecanol, the alkyl glycoside and the sodium lauroyl sarcosine have moisturizing effect, protect hair from being damaged during hair washing, and have repairing effect; the dimethiconol contains hydroxyl and can play a role in conditioning by being matched with the polyquaternium-73, and the sodium benzoate and the phenoxyethanol are preservatives; the cocamide MEA also has a thickening effect.

In addition, in the shampoo provided by the embodiment of the invention, if the components in the anti-dandruff composition and the components in the basic formula are in the following mass fraction ranges, the shampoo has better softening and moisturizing effects while having better anti-dandruff capability.

Specifically, the shampoo provided by the embodiment of the invention comprises the following components in percentage by mass as shown in table 1.

TABLE 1

Substance(s)	Mass fraction	Substance(s)	Mass fraction
				Deionized water	50％～80％	BaylaurelSodium acyl sarcosinate	1％～4％
EDTA％-2Na	0.05％～0.1％	Sodium benzoate	0.1％～0.5％
				Polyquaternium-10	0.05％～0.2％	Polyquaternium-73	0.5％～2％
Guar hydroxypropyl trimethyl ammonium chloride	0.05％～0.2％	Dimethylsiloxanol	1％～8％
				Citric acid	0.05％～0.2％	Phenoxyethanol	0.3％～0.6％
Cocoamidopropyl betaine	2％～10％	Essence	0.1％～0.5％
				Sodium laureth sulfate	5％～20％	Maltotetraose	0.5％～1％
Ammonium lauryl sulfate	1％～8％	Cacumen Platycladi extract	0.01％～0.5％
				Cocoamide MEA	1％～4％	Angelica sinensis extract	0.01％～0.5％
Ethylene glycol distearate	1％～3％	Sophora flavescens extract	0.01％～0.5％
				Octadecanol	0.1％～0.2％	Pyrrolidone ethanolamine salt	0.1％～0.5％
Alkyl glycosides	1％～3％

In a preferred embodiment, the mass fraction of the cacumen biotae extract is 0.08%, the mass fraction of the angelica sinensis extract is 0.16%, the mass fraction of the sophora flavescens extract is 0.32%, and the mass fraction of the pyrrolidone ethanolamine salt is 0.1%, so that the shampoo provided by the embodiment of the invention has an excellent anti-dandruff effect.

In a preferred embodiment, the mass fraction of the cacumen biotae extract is 0.08%, the mass fraction of the angelica sinensis extract is 0.16%, the mass fraction of the sophora flavescens extract is 0.32%, and the mass fraction of the pyrrolidone ethanolamine salt is 0.2%, so that the shampoo provided by the embodiment of the invention has an excellent anti-dandruff effect.

The present invention also provides a method for preparing the shampoo as described above, as shown in fig. 1, the method for preparing the shampoo comprises the steps of:

s100, dispersing the EDTA-2Na, the polyquaternium-10 and the guar propyl trimethyl ammonium chloride in deionized water to obtain a first solution.

S200, heating the first solution, adding the citric acid and the cocamidopropyl betaine to the first solution in sequence, then adding the sodium laureth sulfate, the ammonium laureth sulfate, the cocamide MEA, the ethylene glycol distearate, the stearyl alcohol, the alkyl glycoside and the sodium lauroyl sarcosinate to obtain a second solution, and keeping the temperature at 80-85 ℃.

S300, cooling the second solution to 60-65 ℃, and adding the polydimethylsiloxane alcohol and the polyquaternium-73 to obtain a third solution.

It should be noted that, in an alternative embodiment of the present invention, the dimethiconol selected in the step S200 is emulsified dimethiconol, and if the emulsified dimethiconol is added to the first solution at a temperature of 80 ℃ to 85 ℃, the obtained solution is unstable; preferably, the step S200 should be performed at 60-65 ℃.

In addition, the phenoxyethanol, the cacumen biotae extract, the angelica sinensis extract and the sophora flavescens extract may be inactivated at 60-65 ℃, and preferably, the step S400 is performed at 40-45 ℃, so that the activities of the phenoxyethanol, the cacumen biotae extract, the angelica sinensis extract and the sophora flavescens extract are sufficiently activated, and finally, the shampoo with the anti-dandruff effect is obtained.

Specifically, in the step S200, the heat preservation time of the second solution is 30min to 60 min.

In summary, the embodiments of the present invention provide an anti-dandruff composition, which includes a herbal extract and a malassezia inhibitor at a predetermined mass ratio, and is capable of inhibiting the proliferation of malassezia, repairing scalp, and removing dandruff. Embodiments of the present invention also provide a shampoo comprising a base formulation and the anti-dandruff composition added to the base formulation, wherein the base formulation can provide a mild vehicle for the anti-dandruff composition to achieve a better anti-dandruff effect. The embodiment of the invention also provides a preparation method of the shampoo, and the preparation method is simple in process, low in cost and capable of realizing industrial production.

In addition, the embodiment of the invention also performs a spot-pasting test, a malassezia bacteriostatic ring test and an anti-dandruff effect evaluation on the shampoo obtained by the preparation method of the shampoo.

In a specific embodiment of the present invention, the specific method for testing the patch includes:

collecting subjects.

② selecting the area not more than 50mm ² And a qualified spot test device with the depth of about 1mm, placing 1% diluted test object on a filter paper sheet, placing the filter paper sheet into a small chamber of a spot test device, wherein the dosage is about 0.02 mL-0.025 mL, a control hole is a negative control (distilled water), applying the spot test device with the test object on the back of a test subject by using a hypoallergenic adhesive tape, and lightly pressing the test object by hands to uniformly apply the test object on the skin for 24 hours.

Thirdly, observing the skin reaction according to the standard of the table 2 after 30 minutes (after the indentation disappears), 24 hours and 48 hours of removing the patch device of the tested object, and recording the observation result.

TABLE 2

In the specific embodiment of the invention, the principle of the malassezia bacteriostasis ring test is that the bacteriostat is continuously dissolved and is diffused by agar to form different concentration gradients so as to display the bacteriostasis effect. The experiment judges whether the bacteriostatic ability is possessed or not according to the size of the bacteriostatic ring. The malassezia bacteriostasis ring test comprises the following steps:

preparing the antibacterial tablets: for liquid bacteriostat, a sterile and dry filter paper sheet is taken. Dripping 20 μ L of bacteriostatic agent solution with practical use concentration into each piece, placing the filter paper piece in a clean sterile plate, opening the cover, and oven drying in a warm box (36 deg.C), or naturally drying at room temperature for use.

Preparing a negative control sample: and (4) taking a sterile dry filter paper sheet, dripping 20 mu L of sterile distilled water into each sheet, and drying for later use.

Inoculation of test bacteria, namely dipping test bacteria suspension with the concentration of 5 multiplied by 105 cfu/mL-5 multiplied by 106cfu/mL by using a sterile cotton swab, and evenly smearing the test bacteria suspension on the surface of a nutrient agar medium flat plate for 3 times. For each application 1 time, the plate should be rotated 60 and the cotton swab finally applied around the edge of the plate for one revolution. The plate was covered and dried at room temperature for 5 min.

Fourthly, sticking bacteriostatic agent sample pieces: each test is stuck with 1 infectious bacterium plate, and each plate is stuck with 4 test sample plates and 1 negative control sample plate, and the number of the negative control sample plates is 5. A sample was taken with sterile forceps and placed on the surface of the plate. The distance between the centers of the various pieces is more than 25mm, and the distance between the centers of the various pieces and the periphery of the flat plate is more than 15 mm. After the sample is placed, the sample is lightly pressed by using sterile tweezers to be tightly attached to the surface of the flat plate. The plate is covered, the plate is placed in a 36 ℃ incubator, and the result is observed after 24-48 h of culture. The diameter of the antibacterial ring (including the patch) was measured with a vernier caliper and recorded. The experiment was repeated 3 times. When the inhibition zone is measured, the inhibition zone which is uniform and completely aseptically grows is selected for measurement. The diameter of the catheter is measured by taking the outer edge of the bacteriostatic ring as a boundary.

The evaluation of the malassezia bacteriostasis ring test is judged according to the following contents:

the negative control group should have no bacteriostatic ring, otherwise the test is invalid. If the diameter of the bacteriostatic ring in the experimental group is more than 7mm, the bacteriostatic ring is judged to have bacteriostatic action; if the diameter of the bacteriostatic ring is less than or equal to 7mm, judging that the bacteriostatic action is not exerted; and judging the test to be qualified when the three repeated tests have the antibacterial effect results.

The specific procedure of the evaluation of the anti-dandruff effect of the embodiment of the present invention is to collect volunteers and evaluate the anti-dandruff effect of shampoo, and the evaluation criteria are shown in table 3.

TABLE 3

Anti-dandruff effect	Is very poor	Not good	In general	Good taste	Is very good
						Score value	1 minute (1)	2 is divided into	3 points of	4 is divided into	5 points of

In order to further illustrate the present invention, an anti-dandruff composition, a shampoo and a preparation method thereof provided by the present invention will be described in detail with reference to examples, which should not be construed as limiting the scope of the present invention.

Example 1

An anti-dandruff composition comprises cacumen biotae extract, angelica sinensis extract, radix sophorae flavescentis extract and pyrrolidone ethanolamine salt, wherein the mass ratio of the cacumen biotae extract to the angelica sinensis extract to the radix sophorae flavescentis extract to the pyrrolidone ethanolamine salt is 4: 8: 18: 10.

a shampoo comprises a basic formula and the anti-dandruff composition added into the basic formula, and specifically comprises the following components in percentage by mass as shown in Table 4:

TABLE 4

Substance(s)	Mass percent	Substance(s)	Mass fraction
				Deionized water	64.9％	Sodium lauroyl sarcosinate	2％
EDTA-2Na	0.05％	Sodium benzoate	0.45％
				Polyquaternium-10	0.1％	Polyquaternium-73	1.5％
Guar hydroxypropyl trimethyl ammonium chloride	0.2％	Dimethylsiloxanol	4％
				Citric acid	0.2％	Phenoxyethanol	0.5％
Cocoamidopropyl betaine	6％	Essence	0.5％
				Sodium laureth sulfate	12％	Maltotetraose	1％
Ammonium lauryl sulfate	2％	Cacumen Platycladi extract	0.04％
				Cocoamide MEA	1.5％	Angelica sinensis extract	0.08％
Ethylene glycol distearate	1.5％	Sophora flavescens extract	0.18％
				Octadecanol	0.2％	Pyrrolidone ethanolamine salt	0.1％
Alkyl glycosides	1％

A method of preparing a shampoo as described above comprising the steps of:

S200, heating the first solution, adding the citric acid and the cocamidopropyl betaine to the first solution in sequence, then adding the sodium laureth sulfate, the ammonium laureth sulfate, the cocamide MEA, the ethylene glycol distearate, the stearyl alcohol, the alkyl glycoside and the sodium lauroyl sarcosinate to obtain a second solution, and keeping the second solution at 85 ℃ for 30 min.

S300, cooling the second solution to 60 ℃, and adding the polydimethylsiloxane alcohol and the polyquaternium-73 to obtain a third solution.

S400, cooling the third solution to 45 ℃, and adding the sodium benzoate, the phenoxyethanol, the essence, the maltotetraose, the cacumen biotae extract, the angelica sinensis extract, the sophora flavescens extract and the pyrrolidone ethanolamine salt to obtain milky shampoo with certain thickness and fluidity.

Examples 2 to 4

Examples 2 to 4 are different from example 1 in that: examples 2 to 4 provide anti-dandruff compositions wherein the mass ratio of the cacumen biotae extract, the angelica sinensis extract, the sophora flavescens extract and the pyrrolidone ethanolamine salt is different. In example 2, the mass ratio of the cacumen biotae extract, the angelica sinensis extract, the sophora flavescens extract and the pyrrolidone ethanolamine salt is 8: 16: 32: 10. in example 3, the mass ratio of the cacumen biotae extract, the angelica sinensis extract, the sophora flavescens extract and the pyrrolidone ethanolamine salt is 4: 8: 18: in example 4, the mass ratio of the cacumen biotae extract to the angelica sinensis extract to the sophora flavescens extract to the pyrrolidone ethanolamine salt is 8: 16: 32: 20.

the shampoos provided in examples 2 to 4 were different in the mass percentage of each component, and the mass percentage of each component is shown in table 5.

In the methods for producing shampoos provided in examples 2 to 4, the mass percentages of the respective components used are different, and the mass percentages of the respective components are shown in table 5.

The rest of the raw materials and the process steps of the examples 2 to 4 are completely the same as those of the example 1, and thus the description is omitted.

Comparative examples 1 to 3

Comparative examples 1 to 3 each provide a shampoo different from the shampoos of examples 1 to 4 in that: on the basis of the basic formula, the anti-dandruff composition provided by the embodiment of the invention is not added. The shampoo of comparative example 1 to comparative example 3 had the respective components in mass percentages shown in table 5.

TABLE 5

After the shampoo fragrances of examples 1 to 4 and comparative examples 1 to 3 are prepared, the patches, the malassezia bacteriostatic ring tests and the dandruff removing effect evaluations of examples 1 to 4 are carried out; the shampoos of comparative examples 1 to 3 were subjected to the patch test.

The specific method for testing the patch comprises the following steps:

the method comprises the following steps of firstly, collecting subjects, wherein 32 subjects are collected in the collection of the subjects, 2 subjects are removed, 4 male subjects, 26 female subjects, the minimum age is 24 years old, the maximum age is 58 years old, and the average age is about 36 years old.

The test results of the patch test of examples 1 to 4 and comparative examples 1 to 3 are shown in table 6.

TABLE 6

Example 1	3 out of 30 people suspicious reactions
		Example 2	1 out of 30 people with suspicious reactions
Example 3	8 out of 30 suspicious reactions
		Example 4	4 out of 30 people suspicious reactions
Comparative example 1	2 out of 30 suspicious reactions
		Comparative example 2	7 out of 30 suspicious reactions
Comparative example 3	12 out of 30 people with suspicious reactions

Based on the test data of table 6: comparing example 1 and example 2 with comparative example 2, and comparing example 3 and example 4 with comparative example 3, it can be seen that the number of suspicious reactions in the patch test after adding the herbal extracts (cacumen biotae extract, angelica sinensis extract and sophora flavescens extract) is smaller in the case of the same content of the malassezia inhibitor pyrrolidone ethanolamine salt. Comparing example 1 with example 2, and comparing example 3 with example 4, it can be seen that the higher the content of the herbal extracts (cacumen biotae extract, angelica sinensis extract and sophora flavescens extract) is, the less the number of suspicious reactions in the spot-on test of the corresponding shampoo is, in the case that the content of the malassezia inhibitor pyrrolidone ethanolamine salt is the same, thereby demonstrating that the addition of cacumen biotae extract, angelica sinensis extract and sophora flavescens extract is effective in reducing the irritation caused by the addition of pyrrolidone ethanolamine salt. Comparative example 1 above, which does not contain the anti-dandruff composition provided by the present invention, contains only the base formula, and the patch results of comparative example 1 are 2 suspicious responses, indicating that in the shampoo provided by the present invention, the base formula is relatively mild and can provide a vehicle for the anti-dandruff composition provided by the present invention.

The invention also performs a malassezia bacteriostasis ring test on the shampoos obtained in examples 1-4, and specifically comprises the following steps:

Preparing a negative control sample: taking a sterile dry filter paper sheet, dripping 20 mu l of sterile distilled water into each sheet, and drying for later use.

Inoculation of test bacteria: a sterile cotton swab is dipped in test bacterial suspension with the concentration of 5 multiplied by 105 cfu/mL-5 multiplied by 106cfu/mL, and the test bacterial suspension is evenly smeared on the surface of a nutrient agar culture medium plate for 3 times. For each application 1 time, the plate should be rotated 60 and the cotton swab finally applied around the edge of the plate for one revolution. The plate was covered and dried at room temperature for 5 min.

And fourthly, sticking bacteriostatic agent sample sheets, namely sticking 1 bacterial staining flat plate on each test, sticking 4 test sample sheets and 1 negative control sample sheet on each flat plate, and sticking 5 test sample sheets. A sample was taken with sterile forceps and placed on the surface of the plate. The distance between the centers of the various pieces is more than 25mm, and the distance between the centers of the various pieces and the periphery of the flat plate is more than 15 mm. After the sample is placed, the sample is lightly pressed by using sterile tweezers to be tightly attached to the surface of the flat plate. The plate is covered, the plate is placed in a 36 ℃ incubator, and the result is observed after 24-48 h of culture. The diameter of the antibacterial ring (including the patch) was measured with a vernier caliper and recorded. The experiment was repeated 3 times. When the inhibition zone is measured, the inhibition zone which is uniform and completely aseptically grows is selected for measurement. The diameter of the catheter is measured by taking the outer edge of the bacteriostatic ring as a boundary.

The results of the malassezia bacteriostasis ring test of examples 1-4 are shown in table 7.

TABLE 7

	Malassezia californica antibacterial ring (mm)
		Example 1	30.31
Example 2	42.43
		Example 3	36.23
Example 4	48.56

As is clear from table 7, examples 1 to 4 had good inhibitory effects on malassezia. Among them, the suppression effects of examples 2 and 4 are relatively better.

In the present invention, 120 persons were also recruited as volunteers and divided into four groups, and the anti-dandruff effect of the shampoos prepared in examples 1 to 4 was evaluated in 30 persons per group, and the evaluation results are shown in Table 8.

TABLE 8

As is clear from table 8, examples 1 to 4 all had good chip-removing effects. Among them, the antidandruff effect of example 2 and example 4 is relatively better.

It can be seen from the comprehensive spot-pasting test, the malassezia bacteriostatic ring test and the anti-dandruff effect evaluation that, as a preferable scheme, the shampoos of examples 2 and 4 all have relatively good spot-pasting test results, malassezia inhibition effects and anti-dandruff effects. Further, the malassezia inhibition effect and the anti-dandruff effect of example 4 are relatively superior to those of example 2, but the number of persons who are suspected to be positive on the spot sticker of example 4 is 4, the number of persons who are suspected to be positive on the spot sticker of example 2 is 1, and the formulation price is relatively high since the amount of the pyrrolone ethanolamine salt of example 4 added is twice that of example 2, and therefore, the anti-dandruff regimen of shampoo is more preferable to the anti-dandruff regimen of shampoo.

In conclusion, the anti-dandruff composition and the corresponding shampoo provided by the embodiment of the invention can inhibit the proliferation of malassezia and repair scalp, and have good anti-dandruff effect.

The foregoing is directed to embodiments of the present application and it is noted that numerous modifications and adaptations may be made by those skilled in the art without departing from the principles of the present application and are intended to be within the scope of the present application.

Claims

1. An anti-dandruff composition is characterized by comprising traditional Chinese medicine extracts and a malassezia inhibitor in a predetermined mass ratio, wherein the traditional Chinese medicine extracts comprise a cacumen biotae extract, an angelica sinensis extract and a sophora flavescens extract.

2. The anti-dandruff composition according to claim 1, wherein the mass ratio of the cacumen biotae extract to the angelica sinensis extract to the sophora flavescens extract to the malassezia inhibitor is (1-50): (1-50): (1-50): (10-50).

3. An anti-dandruff composition according to claim 1 or 2, wherein the malassezia inhibitor is pyrrolidone ethanolamine salt.

4. A shampoo comprising a base formulation and an anti-dandruff composition added to said base formulation, said anti-dandruff composition being an anti-dandruff composition according to any one of claims 1 to 3.

5. A shampoo according to claim 4 characterised in that the base formulation comprises the following ingredients: deionized water, EDTA-2Na, polyquaternium-10, guar gum hydroxypropyl trimethyl ammonium chloride, citric acid, cocamidopropyl betaine, sodium laureth sulfate, ammonium laureth sulfate, cocamide MEA, ethylene glycol distearate, stearyl alcohol, alkyl glycoside, sodium lauroyl sarcosinate, sodium benzoate, polyquaternium-73, dimethiconol, phenoxyethanol, essence and maltotetraose.

6. A shampoo as claimed in claim 5 which comprises the following components in parts by weight:

50 to 80 percent of deionized water;

0.05 to 0.1 percent of EDTA-2 Na;

0.05 to 0.2 percent of polyquaternium-10;

0.05 to 0.2 percent of guar hydroxypropyl trimethyl ammonium chloride;

0.05 to 0.2 percent of citric acid;

2-10% of cocamidopropyl betaine;

5 to 20 percent of lauryl alcohol polyether sodium sulfate;

1-8% of ammonium lauryl sulfate;

1% -4% of coconut oil amide MEA;

1-3% of ethylene glycol distearate;

0.1 to 0.2 percent of octadecanol;

1-3% of alkyl glycoside;

1-4% of sodium lauroyl sarcosinate;

0.1 to 0.5 percent of sodium benzoate;

0.5% -2% of polyquaternium-73;

1 to 8 percent of dimethiconol;

0.3 to 0.6 percent of phenoxyethanol;

0.1 to 0.5 percent of essence;

0.5 to 1 percent of maltotetraose;

0.01 to 0.5 percent of biota orientalis leaf extract;

0.01 to 0.5 percent of angelica extract;

0.01 to 0.5 percent of sophora flavescens extract;

0.1% -0.5% of pyrrolidone ethanolamine salt.

7. The shampoo of claim 6, wherein the mass fraction of the cacumen biotae extract is 0.08%, the mass fraction of the angelica sinensis extract is 0.16%, the mass fraction of the sophora flavescens extract is 0.32%, and the mass fraction of the pyrrolidone ethanolamine salt is 0.1%.

8. The shampoo of claim 6, wherein the mass fraction of the cacumen biotae extract is 0.08%, the mass fraction of the angelica sinensis extract is 0.16%, the mass fraction of the sophora flavescens extract is 0.32%, and the mass fraction of the pyrrolidone ethanolamine salt is 0.2%.

9. A process for the preparation of a shampoo as claimed in any of claims 4 to 8, characterised in that it comprises the following steps:

10. The method for preparing a shampoo according to claim 9, wherein the holding time of the second solution in the step S200 is 30min to 60 min.