CN114846136B - 源自鲎的重组G因子及使用该重组G因子的β-葡聚糖的测定方法 - Google Patents

源自鲎的重组G因子及使用该重组G因子的β-葡聚糖的测定方法 Download PDF

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CN114846136B
CN114846136B CN202080086019.6A CN202080086019A CN114846136B CN 114846136 B CN114846136 B CN 114846136B CN 202080086019 A CN202080086019 A CN 202080086019A CN 114846136 B CN114846136 B CN 114846136B
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CN114846136A (zh
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山本阳太郎
北川刚史
福地大树
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Fujifilm Corp
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43509—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from crustaceans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Insects & Arthropods (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080086019.6A 2019-12-11 2020-12-10 源自鲎的重组G因子及使用该重组G因子的β-葡聚糖的测定方法 Active CN114846136B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-223604 2019-12-11
JP2019223604 2019-12-11
PCT/JP2020/046178 WO2021117841A1 (ja) 2019-12-11 2020-12-10 カブトガニ由来組換えＦａｃｔｏｒＧ及びこれを用いたβ－グルカンの測定方法

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CN114846136A CN114846136A (zh) 2022-08-02
CN114846136B true CN114846136B (zh) 2024-07-12

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Country Link
US (1) US12545711B2 (https=)
EP (1) EP4074827A4 (https=)
JP (1) JP7358509B2 (https=)
KR (1) KR102785439B1 (https=)
CN (1) CN114846136B (https=)
WO (1) WO2021117841A1 (https=)

Families Citing this family (1)

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Publication number Priority date Publication date Assignee Title
US20250044296A1 (en) 2021-08-04 2025-02-06 Seikagaku Corporation Reagent for measuring b-glucan, method for producing same and use thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4832134B1 (https=) 1968-09-06 1973-10-04
JP2928287B2 (ja) 1988-09-29 1999-08-03 協和醗酵工業株式会社 新規ポリペプチド
JP2564632B2 (ja) 1988-11-21 1996-12-18 マルハ株式会社 β−グルカン類に対する特異性の高いアメボサイト・ライセート及びその製造方法
JP3652738B2 (ja) 1994-06-30 2005-05-25 生化学工業株式会社 エンドトキシン安定化剤、エンドトキシン組成物およびエンドトキシンの測定法
US7541162B2 (en) * 2005-03-28 2009-06-02 Seikagaku Corporation Method for producing factor G derived from horseshoe crab
US20090208995A1 (en) 2006-07-07 2009-08-20 Seikagaku Corporation Pro-clotting enzyme, and method for detection of endotoxin or (1-3)-beta-d-glucan using the same
JP5686308B2 (ja) * 2009-02-20 2015-03-18 国立大学法人広島大学 βグルカンの濃度測定方法および濃度測定用キット
WO2010107068A1 (ja) 2009-03-17 2010-09-23 和光純薬工業株式会社 β－グルカンの測定方法及びそれに用いられるβ－グルカン結合性蛋白質

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Noriaki Seki等.Horseshoe Crab (1,3)-β-D-Glucan-sensitive Coagulation Factor G.THE JOURNAL OF BIOLOGICAL CHEMISTRY.1994,第269卷(第2期),1370-1374. *
Q27083;Uniprot;Uniprot;全文 *
Uniprot.Q27083.Uniprot.2019,全文. *

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US12545711B2 (en) 2026-02-10
KR20220097504A (ko) 2022-07-07
JP7358509B2 (ja) 2023-10-10
JPWO2021117841A1 (https=) 2021-06-17
US20220306705A1 (en) 2022-09-29
EP4074827A4 (en) 2023-11-01
KR102785439B1 (ko) 2025-03-21
CN114846136A (zh) 2022-08-02
WO2021117841A1 (ja) 2021-06-17
EP4074827A1 (en) 2022-10-19

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