CN114766123A - Tiny seed dibbling device - Google Patents

Tiny seed dibbling device Download PDF

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Publication number
CN114766123A
CN114766123A CN202210426407.5A CN202210426407A CN114766123A CN 114766123 A CN114766123 A CN 114766123A CN 202210426407 A CN202210426407 A CN 202210426407A CN 114766123 A CN114766123 A CN 114766123A
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CN
China
Prior art keywords
pressing
handle
pressing handle
baffle
piston
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210426407.5A
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Chinese (zh)
Inventor
刘国元
李懿鑫
杨杰
果弘毅
储歆彤
罗春影
陈双宜
张健
陈艳红
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Nantong University
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Nantong University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nantong University filed Critical Nantong University
Priority to CN202210426407.5A priority Critical patent/CN114766123A/en
Publication of CN114766123A publication Critical patent/CN114766123A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like

Abstract

The invention discloses a tiny seed dibbling device, which comprises a pressing device, a buffering device and a collecting device, wherein the pressing device is used for pressing a tiny seed dibbling device; the pressing device is connected with the buffering device through the pressing handle, the buffering device is connected with the collecting device through the connecting port, the pressing handle pushes the first pressing handle, the first pressing handle and the connecting handle downwards through pressure, the push-pull device of the buffering device pushes the piston through the downward pressure to buffer the pressing speed, the fourth baffle is pushed to open and close, the diameter of the collecting device is gradually reduced from top to bottom, and the diameter of the lower pipe orifice is 0.05-0.3 cm. In a plant transgenic experiment, seeds with the diameter of 0.05-0.3cm such as disinfected arabidopsis thaliana are placed into the device, a small amount of sterile water is added to be uniformly mixed with the seeds, the seeds are inoculated by pressing the handle, the inoculation process can be completed only through the device, the number of the beated seeds is convenient to control, the culture medium is not easy to puncture through the gun head, and the inoculation efficiency is improved.

Description

Tiny seed dibbling device
Technical Field
The invention relates to a tiny seed dibbling device, and belongs to the technical field of plant culture devices.
Background
Arabidopsis thaliana is also known as Arabidopsis thaliana, and Arabic grass. The genome of Arabidopsis belongs to the phylum angiosperma, the class dicotyledonae, the family Brassicaceae, and contains approximately 12500 kilobase pairs and 5 chromosomes. Arabidopsis is a typical selfing propagation plant, genetic stability is easy to maintain, and mutant phenotype is easy to observe, thereby providing convenience for mutant screening. Meanwhile, artificial hybridization can be conveniently carried out, and genetic research is facilitated.
When the seeds such as arabidopsis thaliana and the like are inoculated to a culture medium, the seeds are very small, and are usually spotted by using a pipette tip or a toothpick after disinfection, so that the culture medium is very easy to puncture, the quantity of the seeds is not easy to control, time and labor are wasted, and efficient dibbling work cannot be carried out. Therefore, there is a need to provide a new type of inoculating device to solve the above problems.
Disclosure of Invention
In view of the problems of the prior art, the present invention provides a tiny seed dibbling device, which can increase the number of arabidopsis seeds during inoculation and increase the inoculation efficiency.
In order to achieve the purpose, the invention adopts the technical scheme that: a tiny seed dibbling device comprises a pressing device, a buffering device and a collecting device; the buffer device is arranged on the main shell of the pressing device; the collecting device is arranged on one side of the pressing device; the pressing device further comprises a pressing handle, a spring I, a pressing handle I, a baffle I and a baffle II; the pressing handle is connected with the first pressing handle; the first pressing handle is connected with a first spring; the first spring (13) is connected between the first baffle plate (15) and the second baffle plate (16); the first baffle plate is fixed on the first pressing handle; the second baffle is fixedly connected to the inner side wall of the main shell and used for limiting the moving distance of the first baffle;
one side of the first pressing handle close to the lower end is connected with the opening and closing device; the opening and closing device comprises a connecting handle, a second pressing handle, a second spring, a bearing, a third baffle, a fourth baffle and a partition plate; the connecting handle is arranged on one side of the pressing handle and is fixed; the second pressing handle is fixedly connected with the connecting handle; the second spring is arranged between the inner side wall of the partition board and the third baffle; the baffle tee joint is hinged on the partition plate through a bearing;
the first pressing handle is connected with the buffering device through the push-pull device; the push-pull device comprises upper meshing teeth arranged on the first pressing handle and lower meshing teeth arranged on a piston rod of the buffer device;
the buffer device also comprises a spring III, a piston and a piston shell for the piston to move up and down; a connecting port is formed at the lower end of the piston shell; the connecting port is connected to the side wall of the collecting device;
the collecting device comprises a cover and a collecting shell; the cover is arranged at the upper end of the collecting shell and is in threaded connection with the shell; the bottom of the collecting shell is abutted against the baffle plate IV.
Furthermore, the lower end of the piston shell of the buffer device is of a slope structure with a certain inclination angle, and the slope terminal of the buffer device is positioned on the connecting port.
Furthermore, the piston and the push-pull device are both made of high-temperature-resistant soft silica gel.
Furthermore, the diameter of the collecting device is gradually reduced from top to bottom, and the diameter of the lower pipe orifice is 0.05-0.3 cm.
The invention has the beneficial effects that: the device can complete the inoculation process, further reduces the probability of contamination caused by contacting other instruments in the inoculation process, is easy to control the number of the seeds of the arabidopsis thaliana, is not easy to puncture a culture medium, and improves the inoculation efficiency.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic structural view of a pressing device and an opening/closing device according to the present invention;
FIG. 3 is a schematic view of a buffer device according to the present invention;
FIG. 4 is a schematic view of the collecting device of the present invention.
In the figure: 1. the device comprises a pressing device 11, a main shell 12, a pressing handle 13, a first spring 14, a first pressing handle 15, a first baffle 16, a second baffle 2, a buffer device 21, a piston rod 22, a third spring 23, a piston 24, a piston shell 241, a connecting port 3, a collecting device 31, a cover 32, a collecting shell 4, an opening and closing device 41, a connecting handle 42, a second pressing handle 43, a second spring 44, a bearing 45, a third baffle 46, a fourth baffle 47, a partition plate 43, a 5 push-pull device 51, an upper meshing tooth 52 and a lower meshing tooth.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood, however, that the detailed description herein of specific embodiments is intended to illustrate the invention and not to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the terms used herein in the specification of the present invention are for the purpose of describing particular embodiments only and are not intended to limit the present invention.
As shown in fig. 1 and 2, a tiny seed dibbling device comprises a pressing device 1, a buffering device 2 and a collecting device 3; the buffer device 2 is arranged on the main shell 11 of the pressing device 1; the collecting device 3 is arranged at one side of the pressing device 1; the pressing device 1 further comprises a pressing handle 12, a first spring 13, a first pressing handle 14, a first baffle 15 and a second baffle 16; the pressing handle 1 is connected with the first pressing handle 14; the first pressing handle 14 is connected with a first spring 13; the first spring (13) is connected between the first baffle plate (15) and the second baffle plate (16); the first baffle plate 15 is fixed on the first pressing handle 14; the second baffle 16 is fixedly connected to the inner side wall of the main shell 11 and is used for limiting the moving distance of the first baffle 15;
continuing to refer to fig. 2, one side of the pressing handle I14 close to the lower end position is connected with the opening and closing device 4; the opening and closing device 4 comprises a connecting handle 41, a second pressing handle 42, a second spring 43, a bearing 44, a third baffle 45, a fourth baffle 46 and a partition plate 47; the connecting handle 41 is arranged on one side of the first pressing handle 14 and is fixed; the second pressing handle 42 is fixedly connected with the connecting handle 41; the second spring 43 is arranged between the inner side wall of the partition plate 47 and the third baffle plate 45; the baffle III 45 is hinged on the side wall of the partition plate 47 through a bearing 44;
the first pressing handle 14 is connected with the buffer device 2 through the push-pull device 5; the push-pull device 5 comprises an upper meshing tooth 51 arranged on the first pressing handle 14 and a lower meshing tooth 52 arranged on the piston rod 21 of the buffer device 2;
referring to fig. 3, the damping device 2 further includes a spring three 22, a piston 23, and a piston housing 24 for the piston 23 to move up and down; a connecting port 241 is arranged at the lower end of the piston shell 24; the connecting port 241 is connected to the side wall of the collecting device 3;
referring to fig. 4, the collecting device 3 includes a cover 31 and a collecting case 32; the cover 31 is arranged at the upper end of the collecting shell 32 and is in threaded connection with the shell; the bottom of the collection housing 32 abuts the baffle plate four 46.
In this structure, the lower end of the piston housing 24 of the damping device 2 preferably has a slope structure with a certain inclination angle, and the slope end thereof is located on the connection port 241.
Preferably, the piston 23 and the push-pull device 5 are made of high-temperature-resistant soft silica gel.
Preferably, the diameter of the collecting device 3 is gradually reduced from top to bottom, and the diameter of the lower pipe orifice is 0.05-0.3 cm.
In this embodiment, the piston and the push-pull device of the pressing device 1, the buffering device 2 and the collecting device 3 are all made of stainless steel, and the piston 23 and the push-pull device 5 are made of high-temperature-resistant soft silica gel. Need carry out effectual washing and sterilization before using this device, in super clean bench, inhale sterile water in with buffer, open lid 31 in this example, pour the good arabidopsis thaliana seed of disinfection into collection device 3, add a little sterile water mixing, press down handle 12, drive and press down handle one 14 and bulldoze downwards, piston rod 21 with being connected of press handle one 14 drives piston 23 downstream, and then form certain air pressure, press down handle one 14 simultaneously and drive through connecting handle 41 and push down press down handle two 42, and then drive baffle three 45 in the baffle 47 and articulate downstream along the bearing, thereby be formed with decurrent pressure to baffle four 46, the seeding mouth is opened in collection shell 32 bottom of collection device 3 this moment, and then reach the purpose of inoculation.
The above description is intended to be illustrative of the preferred embodiment of the present invention and should not be taken as limiting the invention, but rather, the invention is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

Claims (4)

1. A tiny seed dibbling device is characterized by comprising a pressing device (1), a buffering device (2) and a collecting device (3); the buffer device (2) is arranged on a main shell (11) of the pressing device (1); the collecting device (3) is arranged on one side of the pressing device (1); the pressing device (1) further comprises a pressing handle (12), a first spring (13), a first pressing handle (14), a first baffle plate (15) and a second baffle plate (16); the pressing handle (1) is connected with the first pressing handle (14); the first pressing handle (14) is connected with a first spring (13); the first spring (13) is connected between the first baffle plate (15) and the second baffle plate (16); the first baffle (15) is fixed on the first pressing handle (14); the second baffle (16) is fixedly connected to the inner side wall of the main shell (11) and is used for limiting the moving distance of the first baffle (15);
one side of the first pressing handle (14) close to the lower end is connected with the opening and closing device (4); the opening and closing device (4) comprises a connecting handle (41), a second pressing handle (42), a second spring (43), a bearing (44), a third baffle plate (45), a fourth baffle plate (46) and a partition plate (47); the connecting handle (41) is arranged on one side of the first pressing handle (14) and is fixed; the second pressing handle (42) is fixedly connected with the connecting handle (41); the second spring (43) is arranged between the inner side wall of the partition plate (47) and the third baffle plate (45); the baffle III (45) is hinged on the partition plate (47) through a bearing (44);
the first pressing handle (14) is connected with the buffer device (2) through a push-pull device (5); the push-pull device (5) comprises an upper meshing tooth (51) arranged on the first pressing handle (14) and a lower meshing tooth (52) arranged on a piston rod (21) of the buffer device (2);
the buffer device (2) further comprises a spring III (22), a piston (23) and a piston shell (24) for the piston (23) to move up and down; a connecting port (241) is arranged at the lower end of the piston shell (24); the connecting port (241) is connected to the side wall of the collecting device (3);
the collecting device (3) comprises a cover (31) and a collecting shell (32); the cover (31) is arranged at the upper end of the collecting shell (32) and is in threaded connection with the shell; the bottom of the collecting shell (32) abuts against the baffle plate four (46).
2. The tiny seed dibbling device of claim 1, wherein the lower end of the piston housing (24) of the buffering device (2) is in a slope structure with a certain inclination angle, and the slope terminal is positioned on the connecting port (241).
3. The tiny seed dibbling device according to claim 1, wherein said piston (23) and said push-pull device are made of high temperature-resistant soft silica gel.
4. The small seed dibbling device according to claim 1, wherein the diameter of said collecting device (3) is gradually reduced from top to bottom, and the diameter of the lower nozzle is 0.05-0.3 cm.
CN202210426407.5A 2022-04-22 2022-04-22 Tiny seed dibbling device Pending CN114766123A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210426407.5A CN114766123A (en) 2022-04-22 2022-04-22 Tiny seed dibbling device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210426407.5A CN114766123A (en) 2022-04-22 2022-04-22 Tiny seed dibbling device

Publications (1)

Publication Number Publication Date
CN114766123A true CN114766123A (en) 2022-07-22

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ID=82431798

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210426407.5A Pending CN114766123A (en) 2022-04-22 2022-04-22 Tiny seed dibbling device

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4183469A (en) * 1977-11-07 1980-01-15 Weaver Hardy P Hand held seed sower
GB2041898A (en) * 1979-02-21 1980-09-17 Vears T L Precision Pneumatic Seed Drill
JP2004261097A (en) * 2003-03-03 2004-09-24 Hiroshi Yoshihara Seeding machine
CN102652472A (en) * 2012-04-18 2012-09-05 北京农业智能装备技术研究中心 Seeding nozzle with self-cleaning and anti-blocking functions and seeding device with nozzle
CN104380884A (en) * 2014-11-04 2015-03-04 昆明理工大学 Extrusion rotary type seeder
KR20190050465A (en) * 2017-11-03 2019-05-13 대한민국(농촌진흥청장) Potable seeder
CN110637562A (en) * 2019-08-26 2020-01-03 浙江工业大学 Liquid-transfering gun for arabidopsis thaliana
CN111587789A (en) * 2020-06-19 2020-08-28 昆明学院 Arabidopsis thaliana liquid-transfering and seed-distributing device
CN212232097U (en) * 2020-05-20 2020-12-29 四川省中医药科学院 Chinese-medicinal material seeder
CN114128447A (en) * 2021-11-26 2022-03-04 重庆化工职业学院 Magnetic pressure control type arabidopsis dibbling device

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4183469A (en) * 1977-11-07 1980-01-15 Weaver Hardy P Hand held seed sower
GB2041898A (en) * 1979-02-21 1980-09-17 Vears T L Precision Pneumatic Seed Drill
JP2004261097A (en) * 2003-03-03 2004-09-24 Hiroshi Yoshihara Seeding machine
CN102652472A (en) * 2012-04-18 2012-09-05 北京农业智能装备技术研究中心 Seeding nozzle with self-cleaning and anti-blocking functions and seeding device with nozzle
CN104380884A (en) * 2014-11-04 2015-03-04 昆明理工大学 Extrusion rotary type seeder
KR20190050465A (en) * 2017-11-03 2019-05-13 대한민국(농촌진흥청장) Potable seeder
CN110637562A (en) * 2019-08-26 2020-01-03 浙江工业大学 Liquid-transfering gun for arabidopsis thaliana
CN212232097U (en) * 2020-05-20 2020-12-29 四川省中医药科学院 Chinese-medicinal material seeder
CN111587789A (en) * 2020-06-19 2020-08-28 昆明学院 Arabidopsis thaliana liquid-transfering and seed-distributing device
CN114128447A (en) * 2021-11-26 2022-03-04 重庆化工职业学院 Magnetic pressure control type arabidopsis dibbling device

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