CN114128447A

CN114128447A - Magnetic pressure control type arabidopsis dibbling device

Info

Publication number: CN114128447A
Application number: CN202111424714.1A
Authority: CN
Inventors: 蒋文明; 周石洋; 戴妍; 李乐
Original assignee: Chongqing Chemical Industry Vocational College
Current assignee: Chongqing Chemical Industry Vocational College
Priority date: 2021-11-26
Filing date: 2021-11-26
Publication date: 2022-03-04

Abstract

The invention belongs to the field of biotechnology seeding instruments, and particularly relates to a magnetic pressure control type arabidopsis dibbling instrument, so that the technical problems that a large amount of labor force and workload are needed and the seeding time is long in the traditional arabidopsis seeding are solved; the invention consists of a seed cavity (1), a seed outlet (2), a push handle (3), a push rod (4), a push barrel (5), a sleeve (6), a reinforcing rib (7), a spring (8), a push needle (9), a needle cylinder (10), a fixing piece (11), a limiting piece (12), a spring piece (13), a rubber pad (14), a cavity opening (15), a magnetic block (16), a connecting rib (17) and a connecting port (18). The invention has the beneficial technical effects that only one seed is sown at one time, and the sowing is convenient, quick and labor-saving.

Description

Magnetic pressure control type arabidopsis dibbling device

Technical Field

The invention belongs to the field of seeding instruments in the gardening technology, and particularly relates to a magnetic pressure control type arabidopsis dibbling instrument.

Background

Because the arabidopsis is easier to transgenize, the plant volume is smaller, the life cycle is short, and the planting is convenient, the arabidopsis is a common model plant in the biotechnology and is generally used for expression and identification of transgenic plants; it is often used for the identification of some plant phenotypes and requires extensive trial repetition to ensure the accuracy of the data; for example, some root length, lateral root number, positive selection for transgenesis, require extensive seeding on plates with medium; because the root system of the arabidopsis thaliana is fragile, some arabidopsis thaliana needs to be transferred and some arabidopsis thaliana needs to be measured after being sowed on a plate, the root of the arabidopsis thaliana cannot be crossed so as to avoid influencing identification, transplantation and survival after transplantation; therefore, certain discreteness is required between seeds when planting; the seeds of the arabidopsis are small, and the discrete planting is very inconvenient and requires very skillful skill and patience.

Two common seeding methods are available: (1) seeding with a liquid transfer gun, and (2) seeding with a gun head of the liquid transfer gun. In the first method, because the liquid-transfering gun is difficult to control, only one batch of seeds can be firstly scattered on a culture medium, and then the seeds are scattered by the gun head of the liquid-transfering gun; the second method needs to seal the upper end of the head of the pipette with fingers for dibbling, can sow one seed, but needs to accurately control the air leakage of the sealing fingers, so a more skillful and accurate method is required, and the second method often causes the phenomenon of more seedings at the same position due to the fatigue of hands, so the head of the pipette needs to be used for scattering; in such biological tests, if there is infection by a parasitic bacterium, the test will fail; the two methods both need long time, need a large amount of labor and workload, have long sowing time, and are very easy to cause test infection to cause test failure; therefore, a convenient, rapid and labor-saving seeder is urgently needed to be used for seeding.

Disclosure of Invention

The invention aims to solve the technical problems of low sowing efficiency, long sowing time and large workload of the traditional arabidopsis thaliana plain sowing machine, and provides an arabidopsis thaliana dibbler which is convenient to sow and high in sowing speed; the device is characterized by comprising a seed cavity 1, a seed outlet 2, a push handle 3, a push rod 4, a push barrel 5, a sleeve 6, a reinforcing rib 7, a spring 8, a push needle 9, a needle barrel 10, a fixing piece 11, a limiting piece 12, a spring piece 13, a rubber pad 14, a cavity opening 15, a magnetic block 16, a connecting rib 17 and a connecting opening 18.

The sleeve 6 is made of a non-metal transparent material, a push cylinder 5 is arranged in the sleeve, a push cylinder 15 is hollow and made of a stainless steel material capable of adsorbing a magnet, the push cylinder 5 is connected with a push rod 4, the push rod 4 extends out of an opening in the middle of the upper portion of the sleeve 6, and a push handle 3 is arranged on the upper portion of the push rod 4; the lower part of the push cylinder 5 is connected with a push needle 9, and the push needle 9 is reinforced and fixed with the push cylinder 5 through a reinforcing rib 7; the lower part of the push cylinder 5 is connected with a spring 8, and the lower part of the spring 8 is connected to the inner bottom of the sleeve 6.

The push needle 9 is inserted into the needle cylinder 10, and the needle cylinder 10 is inserted from the bottom of the sleeve 6 and fixed on the inner side wall of the sleeve 6; the seed cavity 1 is positioned at one side of the sleeve 6 and is fixedly connected with the sleeve through a connecting rib 17, the lower part of the seed cavity 1 is provided with a connecting port 18 and is communicated with the needle cylinder 10 through the connecting port 18, and the connecting port 18 can only pass through one arabidopsis thaliana seed 19; when the arabidopsis dibbler is in a recovery state, the head of the lower end of the push needle 9 is positioned at the upper side of the internal connecting port 18 of the needle cylinder 10; the top of the seed cavity 1 is provided with a cavity opening 15, and the upper opening of the cavity opening 15 is smooth.

A fixed plate 11 is arranged on the opposite side of the top of the seed cavity 1 connected with the sleeve 6, and the fixed plate 11 is hinged with a spring leaf 13; a limiting piece 12 is arranged near the hinged position of the spring piece 13, a rubber pad 14 is arranged at the lower part of the spring piece 13, and a magnetic block 16 is arranged at the lower part of the other end of the spring piece 13.

When the arabidopsis dibbler is in a recovery state, the attraction force between the magnetic block 16 and the push cylinder 5 is greater than the elastic force of the spring piece 13, the magnetic block 16 and the push cylinder 5 are attracted by separating the sleeve 6, at the moment, the spring piece 13 is bent, and the rubber pad 14 seals the cavity opening 15; when the push handle 3 is pressed down, the elasticity of the spring piece 13 is larger than the suction force between the magnetic block 16 and the push cylinder 5, the spring piece 13 stretches, one end of the rubber pad 14 is supported at the edge of the cavity opening 15, and the other end is opened, so that the seed cavity 1 is communicated with the outside atmosphere; the push needle 9 is made of opaque material, and the seed cavity 1 is made of transparent material. The elastic force of the spring piece 13 does not need to be too large so as to prevent violent vibration caused by too large elastic force during adsorption and desorption, and the elastic force can be adjusted by selecting different thicknesses or widths so as to ensure that the magnetic block 16 can be bounced after the push cylinder 5 moves downwards.

The beneficial technical effects of the invention are as follows:

(1) only one seed is produced by pressing the push handle once, so that excessive dibbling is avoided once, and the speed is high, so that the test operation time is shortened, and the pollution probability in the test is reduced. (2) Only the handle is needed to be pressed, so the dibbling speed is high, and skillful manipulations and skills are not needed. (3) When the push handle is pressed, the push barrel moves downwards, the spring piece drives the rubber pad to leave the cavity opening after the adsorption force between the magnetic block and the push barrel is smaller than the elastic force of the spring piece, so that the seed cavity is communicated with the outside, and the negative pressure in the seed cavity caused by long-time sowing is avoided, and the seed cannot be sowed. When the push cylinder is restored, the top of the push cylinder is abutted against the inner top of the sleeve, and the adsorption force of the magnetic block and the push cylinder is greater than the elastic force of the spring piece, so that the magnetic block is adsorbed on the outer top of the sleeve, and the rubber pad is driven to seal the cavity opening. When the dibbling operation is not performed for a long time, the dibbling operation can be always in a sealing state, so that the probability of infection of the internal liquid is reduced. The operation of alternately sealing the lower part and the upper part prevents the inside from forming negative pressure, avoids the seeds from moving up and down and also avoids the uncontrollable continuous downward movement of the seeds. (4) The spring piece is provided with a limiting piece, a fixing piece is arranged at the position near the limiting piece, and a gap is formed between the limiting piece and the fixing piece; the design limits the spring piece to have a larger angle and can not restore the state of the adsorption push cylinder.

Drawings

FIG. 1 is a front view of an Arabidopsis thaliana dibbler;

FIG. 2 is a schematic view of portion A of FIG. 1;

FIG. 3 is a schematic view of portion A of FIG. 1 after the handle has been depressed;

FIG. 4 is a schematic view of portion B of FIG. 1;

FIG. 5 is a schematic view of a portion B of FIG. 1 when seeded;

in the figure, 1-seed cavity, 2-seed outlet, 3-push handle, 4-push rod, 5-push cylinder, 6-sleeve, 7-reinforcing rib, 8-spring, 9-push needle, 10-syringe, 11-fixing piece, 12-limiting piece, 13-spring piece, 14-rubber pad, 15-cavity opening, 16-magnetic block, 17-connecting rib, 18-connecting opening and 19-arabidopsis seed.

Detailed Description

The specific structure of the present invention will be further described with reference to fig. 1-5 and the manner of use.

The structure and the principle of an arabidopsis dibbling device are shown in figures 1-5, the dibbling device consists of a seed cavity 1, a seed outlet 2, a push handle 3, a push rod 4, a push cylinder 5, a sleeve 6, a reinforcing rib 7, a spring 8, a push needle 9, a needle cylinder 10, a fixing piece 11, a limiting piece 12, a spring piece 13, a rubber pad 14, a cavity opening 15, a magnetic block 16, a connecting rib 17 and a connecting port 18; wherein, sleeve 6 is made for non-metallic transparent material, and its inside has a push jack 5, and push jack 15 is inside cavity, is made by the stainless steel material that can adsorb magnet, and push jack 5 is connected with push rod 4, and push rod 4 stretches out from the opening in the middle of sleeve 6 upper portion, and push rod 4 upper portion is provided with and pushes away handle 3.

The lower part of the push cylinder 5 is connected with a push needle 9, and the push needle 9 is reinforced and fixed with the push cylinder 5 through a reinforcing rib 7. The lower part of the push cylinder 5 is connected with a spring 8, and the lower part of the spring 8 is connected to the inner bottom of the sleeve 6; the push needle 9 is inserted into the needle cylinder 10, and the needle cylinder 10 is inserted from the bottom of the sleeve 6 and fixed on the inner side wall of the sleeve 6.

The seed cavity 1 is positioned at one side of the sleeve 6 and is fixedly connected with the sleeve through a connecting rib 17, the lower part of the seed cavity 1 is provided with a connecting port 18 and is communicated with the needle cylinder 10 through the connecting port 18, and the connecting port 18 can only pass through one arabidopsis thaliana seed; when the arabidopsis thaliana dibbler is in a recovery state, the head of the lower end of the push needle 9 is positioned above the internal connection port 18 of the needle cylinder 10. The top of the seed cavity 1 is provided with a cavity opening 15, and the upper opening of the cavity opening 15 is smooth; a fixed plate 11 is arranged on the opposite side of the top of the seed cavity 1 connected with the sleeve 6, and the fixed plate 11 is hinged with a spring leaf 13; a limiting piece 12 is arranged near the hinged position of the spring piece 13, a rubber pad 14 is arranged at the lower part of the spring piece 13, and a magnetic block 16 is arranged at the lower part of the other end of the spring piece 13; when the arabidopsis dibbler is in a recovery state, the attraction force between the magnetic block 16 and the push cylinder 5 is greater than the elastic force of the spring piece 13, the magnetic block 16 and the push cylinder 5 are attracted by the sleeve 6, at the moment, the spring piece 13 is bent, and the cavity opening 15 is sealed by the rubber pad 14. When the push handle 3 is pressed down, the elasticity of the spring piece 13 is larger than the suction force between the magnetic block 16 and the push cylinder 5, the spring piece 13 stretches, one end of the rubber pad 14 is supported at the edge of the cavity opening 15, and the other end is opened, so that the seed cavity 1 is communicated with the outside atmosphere; the push needle 9 is made of opaque material, and the seed cavity 1 is made of transparent material.

As shown in the attached figures 1 and 5 of the specification, before sowing, the arabidopsis seeds are rinsed, floating immature seeds are removed, and other impurities are removed; the method comprises the following steps of (1) disinfecting arabidopsis seeds, and rinsing after disinfection; pressing the push handle 3, sealing a connecting port 18 communicated between the seed cavity 1 and the needle cylinder 10 by the push needle 9, enabling the seed cavity 1 to be communicated with the outside atmosphere due to the fact that the suction force between the magnetic block 16 and the push cylinder 5 is reduced and the spring piece 13 is changed from bending to stretching, one end of the rubber pad 14 is supported at the edge of the cavity opening 15, and the other end of the rubber pad is opened, and injecting an arabidopsis thaliana seed 19 into the seed cavity 1 of the arabidopsis thaliana dibbler from the cavity opening 15 by using a sterilized liquid transfer gun; the arabidopsis seed 19 falls to the connecting port 18 under the action of gravity, the push handle 3 is loosened to restore the arabidopsis dibbler, the magnetic block 16 and the push cylinder 5 are attracted by the sleeve 6, the spring leaf 13 is bent, and the rubber pad 14 seals the cavity opening 15; when the head of the lower end of the push pin 9 returns to the upper side of the connection port 18 inside the needle cylinder 10, the arabidopsis thaliana seeds 19 enter the needle cylinder 10 from the connection port 18, and the arabidopsis thaliana seeds 19 stay in the needle cylinder 10 at the connection port 18 under the adhesion action of the atmospheric pressure and water.

When dibbling, the push handle 3 is pressed, the push needle 9 pushes one arabidopsis thaliana seed 19 downwards along the needle cylinder 10, the connection port 18 is sealed by the push needle 9, in the downward pushing process, the suction force of the magnetic block 16 and the push cylinder 5 is smaller than the elastic force of the spring leaf 13, the spring leaf 13 is changed from bending to stretching, one end of the rubber pad 14 is supported at the edge of the cavity port 15, the other end of the rubber pad is opened, so that the seed cavity 1 is communicated with the outside atmosphere, and the phenomenon that the inside seed and water move downwards due to the negative pressure formed inside the seed cavity 1 is prevented; continuously pushing the push handle 3, pushing the arabidopsis thaliana seeds 19 out from the seed outlet 2, and dibbling the seeds on a culture medium; then slowly loosening the push handle 3 to enable the arabidopsis dibbling device to be in a recovery state, wherein the attraction force between the magnetic block 16 and the push cylinder 5 is greater than the elastic force of the spring leaf 13, the magnetic block 16 and the push cylinder 5 are attracted by the sleeve 6, the spring leaf 13 is bent, and the rubber pad 14 seals the cavity opening 15; the next arabidopsis seed 19 falls into the needle cylinder 10 at the connection port 18; and then dibbling the next time.

The above description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and for example, the connection manner may be other than the connection column. Any changes or substitutions that may be easily made by those skilled in the art within the technical scope of the present disclosure are intended to be included within the scope of the present disclosure. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A magnetic pressure control type Arabidopsis dibbling device is characterized by comprising a seed cavity (1), a seed outlet (2), a push handle (3), a push rod (4), a push cylinder (5), a sleeve (6), a reinforcing rib (7), a spring (8), a push needle (9), a needle cylinder (10), a fixing piece (11), a limiting piece (12), a spring piece (13), a rubber pad (14), a cavity opening (15), a magnetic block (16), a connecting rib (17) and a connecting port (18);

the sleeve (6) is made of a non-metal transparent material, a push cylinder (5) is arranged in the sleeve, the push cylinder (5) is hollow and made of a stainless steel material capable of adsorbing a magnet, the push cylinder (5) is connected with a push rod (4), the push rod (4) extends out of an opening in the middle of the upper portion of the sleeve (6), and a push handle (3) is arranged on the upper portion of the push rod (4);

the lower part of the push cylinder (5) is connected with a push needle (9), and the push needle (9) is reinforced and fixed with the push cylinder (5) through a reinforcing rib (7); the lower part of the push cylinder (5) is connected with a spring (8), and the lower part of the spring (8) is connected to the bottom in the sleeve (6); the push needle (9) is inserted into the needle cylinder (10), and the needle cylinder (10) is inserted from the bottom of the sleeve (6) and fixed on the inner side wall of the sleeve (6);

the seed cavity (1) is positioned at one side of the sleeve (6) and is fixedly connected with the sleeve through a connecting rib (17), and the lower part of the seed cavity (1) is provided with a connecting port (18) and is communicated with the needle cylinder (10) through the connecting port (18); when the arabidopsis dibbling device is in a recovery state, the head of the lower end of the push needle (9) is positioned at the upper side of the internal connecting port (18) of the needle cylinder (10).

The top of the seed cavity (1) is provided with a cavity opening (15); a fixed plate (11) is arranged on the opposite side of the top of the seed cavity (1) connected with the sleeve (6), and the fixed plate (11) is hinged with a spring leaf (13); a rubber pad (14) is arranged at the lower part of the spring piece (13), and a magnetic block (16) is arranged at the lower part of the other end of the spring piece (13); when the arabidopsis dibbler is in a recovery state, the attraction force between the magnetic block (16) and the push cylinder (5) is greater than the elastic force of the spring piece (13), the magnetic block (16) and the push cylinder (5) are attracted by separating the sleeve (6), the spring piece (13) is bent at the moment, and the rubber pad (14) seals the cavity opening (15); when the push handle (3) is pressed down, the elasticity of the spring piece (13) is larger than the suction force between the magnetic block (16) and the push cylinder (5), the spring piece (13) stretches, one end of the rubber pad (14) is supported at the edge of the cavity opening (15), and the other end is opened, so that the seed cavity (1) is communicated with the outside.

2. A magnetic pressure-controlled Arabidopsis thaliana dibbler according to claim 1, wherein said push pin (9) is made of an opaque material and said seed chamber (1) is made of a transparent material.

3. A magnetic pressure control type Arabidopsis thaliana dibbling device according to claim 1, wherein the connection port (18) can only pass through one Arabidopsis thaliana seed (19) to facilitate single-seed dibbling.

4. A magnetic pressure control type Arabidopsis thaliana dibbler as claimed in claim 1, wherein a limiting piece (12) is arranged near the hinge of the spring piece (13) for limiting the opening angle of the spring piece (13) so as to prevent the spring piece (13) from driving the dibbler to vibrate.

5. A magnetic pressure control type Arabidopsis thaliana dibbling device according to claim 1, wherein the upper opening of the cavity (15) is smooth, which facilitates the sealing of the rubber gasket (14) and the cavity (15).