CN114128448A - Limit control type arabidopsis dibbling device - Google Patents
Limit control type arabidopsis dibbling device Download PDFInfo
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- CN114128448A CN114128448A CN202111424715.6A CN202111424715A CN114128448A CN 114128448 A CN114128448 A CN 114128448A CN 202111424715 A CN202111424715 A CN 202111424715A CN 114128448 A CN114128448 A CN 114128448A
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- seed
- cavity
- push rod
- pressing head
- arabidopsis thaliana
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C7/00—Sowing
- A01C7/02—Hand sowing implements
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention belongs to the field of seeding instruments in the gardening technology, and particularly relates to a limit control type arabidopsis thaliana dibbling instrument. The dibbling device comprises a pressing head (1), a spring (2), a seed cavity (3), a push rod (4) and a seed outlet pipe (5); the pressing head (1) is provided with a bulge (1.1) and a ventilation groove (1.2), the push rod (4) comprises an upper push rod (4.1) and a lower push rod (4.2), and the lower part of the upper push rod (4.1) is provided with a pressure relief hole (4.3) which is communicated with the ventilation groove (1.2); the lower end part of the lower push rod (4.2) is provided with a seed taking groove (4.4), and part of the end part of the seed taking groove extends into the seed outlet pipe (5); the top of the seed cavity (3) is provided with a cavity opening (3.2) and is opened and closed through a cavity cover (3.3); the cavity bottom (3.6) is obliquely arranged towards the direction of the seed outlet pipe (5). The invention has the beneficial technical effects that only one seed is sown at one time, and the sowing is convenient, quick and labor-saving.
Description
Technical Field
The invention belongs to the field of seeding instruments in the gardening technology, and particularly relates to a limit control type arabidopsis thaliana dibbling instrument.
Background
Because the arabidopsis is easier to transgenize, the plant volume is smaller, the life cycle is short, and the planting is convenient, the arabidopsis is a common model plant in the biotechnology and is generally used for expression and identification of transgenic plants; it is often used for the identification of some plant phenotypes and requires extensive trial repetition to ensure the accuracy of the data; for example, some root length, lateral root number, positive selection for transgenesis, require extensive seeding on plates with medium; because the root system of the arabidopsis thaliana is fragile, after the arabidopsis thaliana is sowed on a plate, some plants need to be transferred, and some plants need to be measured, so that the root of the arabidopsis thaliana cannot be crossed so as to avoid influencing identification, transplanting and survival after transplanting, and therefore, certain discreteness is needed among seeds during planting; the seeds of the arabidopsis are small, and the discrete planting is very inconvenient and requires very skillful skill and patience.
Two common seeding methods are available: (1) seeding with a liquid transfer gun, and (2) seeding with a gun head of the liquid transfer gun. In the first method, because the liquid-transfering gun is difficult to control, only one batch of seeds can be firstly scattered on a culture medium, and then the seeds are scattered by the gun head of the liquid-transfering gun; the second method needs to seal the upper end of the head of the pipette with fingers for dibbling, can perform single-seed sowing, but needs to accurately control the air leakage of the sealed fingers, so a skillful and accurate method is required, and the second method often causes the phenomenon of sowing multiple seeds at one position due to the fatigue of hands, so the head of the pipette needs to be used for dispersing; in such biological tests, if there is infection by a parasitic bacterium, the test will fail; the two methods both need long time, need a large amount of labor and workload, have long sowing time, and are very easy to cause test infection to cause test failure; therefore, a convenient, rapid and labor-saving seeder is urgently needed to be used for seeding.
Disclosure of Invention
Aiming at the technical problem, the invention designs a seeder convenient for seeding arabidopsis thaliana, and the seeder comprises the following specific contents:
the arabidopsis dibbling device is characterized by comprising a pressing head 1, a spring 2, a seed cavity 3, a push rod 4 and a seed outlet pipe 5.
The pressing head 1 is positioned at the upper part of the dibbling device, and the top of the pressing head is provided with a ventilation groove 1.2; the ventilation groove 1.2 leads to the central position of the pressing head 1 and is used for ventilation. The ventilation groove 1.2 can prevent fingers from blocking the ventilation pipeline when the fingers press.
The central position of the lower part of the pressing head 1 is connected with a push rod 4, and the push rod 4 comprises an upper push rod 4.1 and a lower push rod 4.2; the top of the lower push rod 4.2 is fixedly provided with a limiting block 3.4, the upper push rod 4.1 is positioned outside the seed cavity and the lower push rod is positioned in the seed cavity 4.1 under the state of no pressing; the lower part of the upper push rod 4.1 is provided with a pressure relief hole 4.3, and the pressure relief hole 4.3 is communicated with the ventilation groove 1.2 at the top of the pressing head 1 through a pore channel inside the upper push rod 4.1.
The lower end part of the lower push rod 4.2 is provided with a seed taking groove 4.4, and a part of the lower end part of the lower push rod 4.2 extends into the seed outlet pipe 5.
A spring 2 is arranged between the pressing head 1 and the seed cavity 3.
The main body of the seed cavity 3 is a cavity wall 3.1, the top of the main body is a cavity top 3.5, a cavity opening 3.2 is arranged at the upper part of the cavity top 3.5, the cavity opening 3.2 is opened and closed through a cavity cover 3.3, and a solution and an arabidopsis thaliana seed are placed in the seed cavity 3; the lower part of the seed cavity 3 is a cavity bottom 3.6, the cavity bottom 3.6 is communicated with the upper part of the seed outlet pipe 5, and the cavity bottom 3.6 is obliquely arranged towards the direction of the seed outlet pipe 5 so as to facilitate the arabidopsis thaliana seeds to slide towards the seed taking groove 4.4 under the action of gravity; the seeds can be brought by the seed taking groove 4.4 towards the seed outlet 5.1.
The seed cavity 3 is made of transparent materials, and consumption conditions of seeds and solution inside the seed cavity can be observed.
The seed receiving tank 4.4 can accommodate only one arabidopsis seed volume.
The upper part of the spring 2 is connected to the bottom of the pressing head 1 and the lower part thereof is connected to the top of the seed cavity 3.
The upper surface of the pressing head 1 is provided with a bulge 1.1 for preventing the hand from sliding in the seeding process.
A dispersant (e.g., Tween) is added to the solution in which the seeds of Arabidopsis thaliana are suspended to prevent the seeds of Arabidopsis thaliana from sticking to each other and being unfavorable for sowing.
The beneficial technical effects of the invention are as follows:
(1) only one seed is discharged by pressing the pressing head once, so that excessive dibbling can not be caused once, and the speed is high, thereby shortening the test operation time and reducing the pollution probability in the test. Only the handle is needed to be pressed, so the dibbling speed is high, and skillful manipulations and skills are not needed.
(2) Be provided with the pressure relieving hole, when the push rod pushed down, the pressure relieving hole can enter into the seed intracavity, just so makes the seed chamber communicate the external world through the cavity pipeline in the last push rod, prevents that the internal back inside negative pressure that forms of many times of seeding of seed chamber from and unable seeding.
(3) The ventilative groove at press head top, the ventilative groove on press head upper portion is connected through last push rod in the pressure release hole, and the design of ventilative groove parallel press head can prevent to be blockked up when the finger is pressed, has guaranteed going on in succession of dibbling.
(4) Be provided with the stopper on the push rod, prevent to press when too fast, the spring drives the push rod and outwards removes too fast to make the push rod lower extreme dash out the seed tube.
Drawings
FIG. 1 is a front view of an Arabidopsis thaliana dibbler;
FIG. 2 is an enlarged schematic view of portion A of FIG. 1;
FIG. 3 is an enlarged schematic view of portion B of FIG. 1;
FIG. 4 is an enlarged schematic view of a portion C of FIG. 1;
in the figure, 1-pressing head, 2-spring, 3-seed cavity, 4-push rod, 5-seed outlet pipe, 1.1-bulge, 1.2-ventilation groove, 3.1-cavity wall, 3.2-cavity mouth, 3.3-cavity cover, 3.4-limiting block, 3.5-cavity top, 3.6-cavity bottom, 4.1-upper push rod, 4.2-lower push rod, 4.3-pressure relief hole, 4.4-seed taking groove and 5.1-seed outlet.
Detailed Description
The specific structure of the present invention will be further described with reference to the accompanying drawings and examples.
The arabidopsis dibbling device is characterized by comprising a pressing head 1, a spring 2, a seed cavity 3, a push rod 4 and a seed outlet pipe 5.
The pressing head 1 is positioned at the upper part of the dibbling device, and the top of the pressing head is provided with a ventilation groove 1.2; the ventilation groove 1.2 leads to the central position of the pressing head 1 and is used for ventilation.
The central position of the lower part of the pressing head 1) is connected with a push rod 4, and the push rod 4 comprises an upper push rod 4.1 and a lower push rod 4.2; a limiting block 3.4 is fixedly arranged on the lower push rod 4.2, the upper push rod 4.1 is positioned outside the seed cavity and the lower push rod is positioned in the seed cavity 4.1 under the state of no pressing; the lower part of the upper push rod 4.1 is provided with a pressure relief hole 4.3, and the pressure relief hole 4.3 is communicated with the ventilation groove 1.2 at the top of the pressing head 1 through a pore channel inside the upper push rod 4.1.
The lower end part of the lower push rod 4.2 is provided with a seed taking groove 4.4, and part of the end part of the seed taking groove extends into the seed outlet pipe 5;
a spring 2 is arranged between the pressing head 1 and the seed cavity 3.
The main body of the seed cavity 3 is a cavity wall 3.1, the top of the main body is a cavity top 3.5, a cavity opening 3.2 is arranged at the upper part of the cavity top 3.5, the cavity opening 3.2 is opened and closed through a cavity cover 3.3, and a solution and an arabidopsis thaliana seed are placed in the seed cavity 3; the lower part of the seed cavity 3 is a cavity bottom 3.6, the cavity bottom 3.6 is communicated with the upper part of the seed outlet pipe 5, and the cavity bottom 3.6 is obliquely arranged towards the direction of the seed outlet pipe 5 so as to facilitate the arabidopsis thaliana seeds to slide towards the seed taking groove 4.4 under the action of gravity; the seeds can be brought by the seed taking groove 4.4 towards the seed outlet 5.1.
The seed cavity 3 is made of transparent materials, and consumption conditions of seeds and solution inside the seed cavity can be observed.
The seed receiving tank 4.4 can accommodate only one arabidopsis seed volume.
The upper part of the spring 2 is connected to the bottom of the pressing head 1 and the lower part thereof is connected to the top of the seed cavity 3.
The upper surface of the pressing head 1 is provided with a bulge 1.1 for preventing the hand from sliding in the seeding process.
The dispersing agent is added into the solution for suspending the arabidopsis seeds to prevent the arabidopsis seeds from being adhered to each other to be unfavorable for sowing.
The specific use mode is as follows:
before dibbling, firstly, sterilizing arabidopsis seeds, and then putting the sterilized seeds into a sterile tween solution; the solution with the arabidopsis seeds was added from the orifice into the seed cavity using a pipette and then capped with a cavity cap.
In the clean bench, the whole dibbling device is vertically placed. Under the action of gravity, the seeds are deposited to the bottom of the seed cavity in the solution, and the inclined surface of the cavity bottom makes the seeds slide to the seed taking groove.
The culture medium plate after the cooling solidification is prepared in advance in the super clean bench, the bottom box of the plate is held by one hand, the dibbling device is held by the other hand, the pressing head is pressed, so that a single seed is pushed out from the seed outlet, the seed falls on the culture medium by utilizing the adhesive force of the culture medium and water, and if the artificial seed does not fall on a target position, the position of the seed outlet is adopted, and the seed is stirred to move to the target position.
The seed cavity adopts transparent material, can observe the distribution condition of inside seed, if at the seeding in-process, because the seed is gathered outside the groove of getting seeds, and does not have the seed in the groove of getting seeds, then need shake the disseminator slightly in order to break the gathering phenomenon of seed for single seed enters into in the groove of getting seeds and in order to carry out normal seeding.
The above description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and for example, the connection manner may be other than the connection column. Any changes or substitutions that may be easily made by those skilled in the art within the technical scope of the present disclosure are intended to be included within the scope of the present disclosure. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (6)
1. A limit control type arabidopsis thaliana dibbling device is characterized by comprising a pressing head (1), a spring (2), a seed cavity (3), a push rod (4) and a seed outlet pipe (5);
the pressing head (1) is positioned at the upper part of the dibbling device, and the top of the pressing head is provided with a ventilation groove (1.2); the ventilation groove (1.2) is communicated with the central position of the pressing head (1) and is used for ventilation;
the central position of the lower part of the pressing head (1) is connected with a push rod (4), and the push rod (4) comprises an upper push rod (4.1) and a lower push rod (4.2); a limiting block (3.4) is fixedly arranged at the top of the lower push rod (4.2), the upper push rod (4.1) is positioned outside the seed cavity, and the lower push rod is positioned in the seed cavity (4.1) in a non-pressing state; a pressure relief hole (4.3) is formed in the lower portion of the upper push rod (4.1), and the pressure relief hole (4.3) is communicated with a ventilation groove (1.2) in the top of the pressing head (1) through a pore channel in the upper push rod (4.1);
the lower end part of the lower push rod (4.2) is provided with a seed taking groove (4.4), and part of the end part of the seed taking groove extends into the seed outlet pipe (5);
a spring (2) is arranged between the pressing head (1) and the seed cavity (3); the spring (2) is sleeved on the upper push rod (4.1);
the main body of the seed cavity (3) is a cavity wall (3.1), the top of the main body is a cavity top (3.5), a cavity opening (3.2) is formed in the upper portion of the cavity top (3.5), the cavity opening (3.2) is opened and closed through a cavity cover (3.3), and a solution and an arabidopsis thaliana seed are placed in the seed cavity (3). The lower part of the seed cavity (3) is a cavity bottom (3.6), the cavity bottom (3.6) is connected with the upper part of the seed outlet pipe (5), and the cavity bottom (3.6) is obliquely arranged towards the direction of the seed outlet pipe (5) so that the arabidopsis thaliana seeds slide towards the seed taking groove (4.4) under the action of gravity; the seeds can be brought to the seed outlet (5.1) by the seed taking groove (4.4).
2. The limit control type arabidopsis thaliana dibbling device according to claim 1, characterized in that the seed cavity (3) is made of transparent material, and the consumption condition of the internal seeds and the solution can be observed.
3. A limit controlled arabidopsis thaliana dibbler according to claim 1, wherein said seed receiving chamber (4.4) is capable of receiving only one arabidopsis thaliana seed.
4. A limit controlled Arabidopsis thaliana dibbler according to claim 1, wherein the spring (2) is connected at its upper portion to the bottom of the pressing head (1) and at its lower portion to the top of the seed chamber (3).
5. The spacing control type arabidopsis thaliana dibbling device according to claim 1, wherein the upper surface of the pressing head (1) is provided with a bulge (1.1) for preventing the occurrence of hand slip during dibbling.
6. The spacing control type arabidopsis thaliana dibbling device according to claim 1, wherein a dispersing agent is added into the solution to prevent arabidopsis thaliana seeds from being adhered to each other and being unfavorable for sowing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111424715.6A CN114128448A (en) | 2021-11-26 | 2021-11-26 | Limit control type arabidopsis dibbling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111424715.6A CN114128448A (en) | 2021-11-26 | 2021-11-26 | Limit control type arabidopsis dibbling device |
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| Publication Number | Publication Date |
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| CN114128448A true CN114128448A (en) | 2022-03-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111424715.6A Withdrawn CN114128448A (en) | 2021-11-26 | 2021-11-26 | Limit control type arabidopsis dibbling device |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105874980A (en) * | 2016-05-11 | 2016-08-24 | 安徽龙王山农业股份有限公司 | Manual seeding device |
| CN206743828U (en) * | 2017-06-02 | 2017-12-15 | 郭爱英 | A kind of nursery card punch |
| CN108353578A (en) * | 2018-04-27 | 2018-08-03 | 佛山市翔桂农机科技有限公司 | One kind can uniform blanking type novel agricultural seeding apparatus |
| CN109511319A (en) * | 2018-12-12 | 2019-03-26 | 安徽新华学院 | Foot-operated gardens excavating gear |
| CN208657305U (en) * | 2018-08-24 | 2019-03-29 | 黑龙江省农垦科学院 | A kind of crop laser normalization seeding row spacing partition device |
| CN110122014A (en) * | 2019-04-22 | 2019-08-16 | 华南农业大学 | A kind of Portable pneumatic rotating down pressing fertilizer applicator |
| CN211152709U (en) * | 2019-11-21 | 2020-08-04 | 道真仡佬族苗族自治县万福农业综合开发有限公司 | Chinese-medicinal material seeder |
-
2021
- 2021-11-26 CN CN202111424715.6A patent/CN114128448A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105874980A (en) * | 2016-05-11 | 2016-08-24 | 安徽龙王山农业股份有限公司 | Manual seeding device |
| CN206743828U (en) * | 2017-06-02 | 2017-12-15 | 郭爱英 | A kind of nursery card punch |
| CN108353578A (en) * | 2018-04-27 | 2018-08-03 | 佛山市翔桂农机科技有限公司 | One kind can uniform blanking type novel agricultural seeding apparatus |
| CN208657305U (en) * | 2018-08-24 | 2019-03-29 | 黑龙江省农垦科学院 | A kind of crop laser normalization seeding row spacing partition device |
| CN109511319A (en) * | 2018-12-12 | 2019-03-26 | 安徽新华学院 | Foot-operated gardens excavating gear |
| CN110122014A (en) * | 2019-04-22 | 2019-08-16 | 华南农业大学 | A kind of Portable pneumatic rotating down pressing fertilizer applicator |
| CN211152709U (en) * | 2019-11-21 | 2020-08-04 | 道真仡佬族苗族自治县万福农业综合开发有限公司 | Chinese-medicinal material seeder |
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Application publication date: 20220304 |