CN102061259B - Plant transgenic treatment device - Google Patents
Plant transgenic treatment device Download PDFInfo
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- CN102061259B CN102061259B CN 201010564695 CN201010564695A CN102061259B CN 102061259 B CN102061259 B CN 102061259B CN 201010564695 CN201010564695 CN 201010564695 CN 201010564695 A CN201010564695 A CN 201010564695A CN 102061259 B CN102061259 B CN 102061259B
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Abstract
The invention discloses a plant transgenic treatment device in the technical field of biology. The device comprises a bacteria removal culture medium, a selective culture medium, a rooting culture medium, an air pump, a gas flow meter, an incubator, a waste liquid bottle and a liquid pump, wherein the bacteria removal culture medium, the selective culture medium and the rooting culture medium are connected in parallel with the liquid pump respectively and connected with the upper inlet of the incubator; the air pump and the gas flow meter are sequentially connected with the lower inlet of the incubator; and the input end of the waste liquid bottle is connected with the bottom outlet of the incubator. By using the device, a receptor material co-cultured with agrobacterium tumfaciens is inoculated to the liquid incubator capable of controlling the growth conditions, and the purposes of bacteria removal, screening, rooting, seedling adaptation and the like are fulfilled by changing different culture media.
Description
Technical field
What the present invention relates to is a kind of device of biological technical field, specifically a kind of plant transgenic treatment device.
Background technology
Plant transgenic technology is the focus of the exploitation of International Agriculture biotechnology research and competition, also is the important channel that ensures China's grain security, ecological safety and increasing peasant income.Whether the method for foreign gene genetic transformation plant needs can be divided into two large classes by tissue culture, regeneration plant by it, and the first kind need to be passed through Regenerated Plantlets, and method commonly used has Agrobacterium_mediated method, particle bombardment etc.; Another kind of method does not need by tissue culture, and at present comparative maturity mainly contains pollen tube passage method, Ovary injection etc.Agrobacterium-mediated transformation is the predominant methods of present dicotyledon gene transduction.This method processing ease, cost is low, transformation efficiency is high, single copy gene integration rate height and foreign gene are more stable in the transfer-gen plant offspring.Yet the shortcoming of Agrobacterium_mediated method also clearly, and it is poly-too loaded down with trivial details that transforms the step exactly, and workload is huge, thereby causes the waste of labor cost.
Summary of the invention
The present invention is directed to the prior art above shortcomings, a kind of plant transgenic treatment device is provided, the acceptor material after cultivating altogether with Agrobacterium, be inoculated in the liquid culture case that to control growth conditions, by changing different substratum, reach degerming, screen, take root, the purpose such as hardening.Compared with prior art, the characteristics of this device tool mainly are: vegetable material keeps motionless in the sterile culture case, the substratum such as change accordingly long-living, degerming in different period, screen, take root is prepared various substratum repeatedly thereby save, and inoculation waits the step poly-repeatedly.Use in addition this device once can inoculate a large amount of explants, postvaccinal explant is under equal conditions cultivated, and saves cost, raises the efficiency, and has eliminated the error that ordinary method causes owing to different batches.
The present invention is achieved by the following technical solutions, the present invention includes: except bacterium culture medium, selection substratum, root media, air pump, gas meter, incubator, waste liquid bottle and liquor pump, wherein: link to each other except bacterium culture medium, selection substratum and root media difference parallel connection liquid pump and with the upper entrance of incubator, air pump, gas meter link to each other with the bottom entrance of incubator successively, and the input terminus of waste liquid bottle links to each other with the outlet at bottom of incubator.
Described incubator comprises: casing, culture bed, hermatic door, seal buckle, gas blowout head and sealed strip, wherein: the gas blowout head is fixedly installed on the interior ingress, bottom of casing and is connected with liquor pump, the culture bed box house that is fixedly installed on, one side of hermatic door and casing is rotationally connected, two seal buckles are arranged at respectively on hermatic door and the casing, and sealed strip is arranged on the hermatic door and the contacted side of casing edge.
Described culture bed be the drawer type device, the top of this drawer type device is distributed with netted stainless steel spring silk and consists of the square opening of the length of side-millimeter;
The bottom of described hermatic door is provided with hinge, and the bottom surface of hermatic door and casing is on the same plane after hermatic door is opened from top to bottom.
Described seal buckle is two magnetic iron blocks of tool.
This device is linked in sequence this device first when using, carry out sterilising treatment again, opens seal buckle and hermatic door after sterilization finishes under gnotobasis, it is culture bed to pull out drawer type, then the vegetable material after the common cultivation be inoculated into culture bed on, and fix with the spring net support.After inoculation is finished, back in the incubator culture bed again, shut hermatic door and seal buckle.Using in the liquid pump suction incubator except bacterium culture medium, open the ventilation switch at last, place thermostatic chamber to cultivate.
After one week of degerming, take off the clip on the waste liquid bottle, remove discarded nutrient solution, then clip is clipped on the waste liquid bottle.After repeating poly-degerming of above-mentioned step 2-3 time, again nutrient solution is changed respectively into selection substratum and root media, carry out the screening of transformant and take root, hardening.
Integral body of the present invention is an aseptic system, as long as nutrient solution is sufficient, transfer-gen plant can be grown in this incubator for a long time.In specific situation, can also change continuously nutrient solution, promote plant Fast Growth in the case, shorten incubation time.
Description of drawings
Fig. 1 is structural representation of the present invention.
Embodiment
The below elaborates to embodiments of the invention, and present embodiment is implemented under take technical solution of the present invention as prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
As shown in Figure 1, present embodiment comprises: except bacterium culture medium 1, selection substratum 2, root media 3, air pump 4, gas meter 5, incubator 6, waste liquid bottle 7 and liquor pump 8, wherein: link to each other except bacterium culture medium 1, selection substratum 2 and root media 3 difference parallel connection liquid pumps 8 and with the upper entrance of incubator 6, air pump 4, gas meter 5 link to each other with the bottom entrance of incubator 6 successively, and the input terminus of waste liquid bottle 7 links to each other with the outlet at bottom of incubator 6.
Described except being equipped with switch folder 9 between bacterium culture medium 1, selection substratum 2 and root media 3 and the liquor pump 8 and on the input terminus of waste liquid bottle 7.
The top of the output terminal of the output terminal of described gas meter 5, waste liquid bottle 7 and incubator 6 is respectively equipped with air filter 10,
Described gas blowout 15 is the glass filter core in 100 microns in aperture.
Described incubator 6 comprises: casing 11, culture bed 12, hermatic door 13, seal buckle 14, gas blowout 15 and sealed strip 16, wherein: gas blowout 15 is fixedly installed on the ingress, bottom in the casing 11 and links to each other with gas meter 5 with air filter 10, culture bed 12 are fixedly installed on casing 11 inside, hermatic door 13 is rotationally connected with a side of casing 11, two seal buckles 14 are arranged at respectively on hermatic door 13 and the casing 11, and sealed strip 16 is arranged on the hermatic door 13 and casing 11 contacted side edges.
Described casing 11 and hermatic door 13 usefulness colourless transparent glass are made
Described culture bed 12 is the drawer type device, and the top of this drawer type device is distributed with netted stainless steel spring silk and consists of the square opening of length of side 1-2 millimeter;
The bottom of described hermatic door 13 is provided with hinge 17, and hermatic door 13 is on the same plane with the bottom surface of casing 11 after hermatic door 13 is opened from top to bottom.
Described seal buckle 14 is two magnetic iron blocks of tool;
Present embodiment is cultivated with the soybean cotyledon node that this device soaked Agrobacterium, is 10 liters by incubator 6 volumes are set, 2000 of inoculation soybean cotyledon nodes.Sterilization twice, each 7 days; Screening operation 2 times, each 30 days; Took root 20 days, hardening 20 days survives after the transfer and reaches 80%.
The device that uses in the present embodiment is applicable to the transgeneic procedure of majority of plant, has very large using value.
Claims (5)
1. plant transgenic treatment device, it is characterized in that, the acceptor material after cultivating altogether with Agrobacterium, be inoculated in the liquid culture case that to control growth conditions, comprise: except bacterium culture medium, select substratum, root media, air pump, gas meter, incubator, waste liquid bottle and liquor pump, wherein: except bacterium culture medium, select substratum to link to each other with root media difference parallel connection liquid pump and with the upper entrance of incubator, air pump, gas meter links to each other with the bottom entrance of incubator successively, and the input terminus of waste liquid bottle links to each other with the outlet at bottom of incubator; Described except being equipped with the switch folder between bacterium culture medium, selection substratum and root media and the liquor pump and on the input terminus of waste liquid bottle; The top of the output terminal of described gas meter, the output terminal of waste liquid bottle and incubator is respectively equipped with the air filter.
2. plant transgenic treatment device according to claim 1, it is characterized in that, described incubator comprises: casing, culture bed, hermatic door, seal buckle, gas blowout head and sealed strip, wherein: the gas blowout head is fixedly installed on the interior ingress, bottom of casing and is connected with the gentle flowmeter body of air filter, the culture bed box house that is fixedly installed on, one side of hermatic door and casing is rotationally connected, two seal buckles are arranged at respectively on hermatic door and the casing, and sealed strip is arranged on the hermatic door and the contacted side of casing edge.
3. plant transgenic treatment device according to claim 2 is characterized in that, described culture bed be the drawer type device, the top of this drawer type device is distributed with netted stainless steel spring silk and consists of the square opening of length of side 1-2 millimeter.
4. plant transgenic treatment device according to claim 2 is characterized in that, the bottom of described hermatic door is provided with hinge.
5. plant transgenic treatment device according to claim 2 is characterized in that, described seal buckle is two magnetic iron blocks of tool.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010564695 CN102061259B (en) | 2010-11-30 | 2010-11-30 | Plant transgenic treatment device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010564695 CN102061259B (en) | 2010-11-30 | 2010-11-30 | Plant transgenic treatment device |
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| Publication Number | Publication Date |
|---|---|
| CN102061259A CN102061259A (en) | 2011-05-18 |
| CN102061259B true CN102061259B (en) | 2013-01-09 |
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| CN 201010564695 Expired - Fee Related CN102061259B (en) | 2010-11-30 | 2010-11-30 | Plant transgenic treatment device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103120125A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method and device for high throughput screening of salt-tolerant plants |
| CN114149919B (en) * | 2021-12-02 | 2023-06-23 | 鲁东大学 | Culture device for cassava genetic information transformation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200952006Y (en) * | 2006-03-30 | 2007-09-26 | 四川大学 | Oral cavity biological membrane dynamic model device |
| CN201065407Y (en) * | 2007-06-14 | 2008-05-28 | 华中科技大学同济医学院附属同济医院 | In vitro cell open type pressure program control culturing device |
| CN101370928A (en) * | 2006-01-17 | 2009-02-18 | 株式会社日立医药 | Cell culture method and automatic culture system using the method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101370928A (en) * | 2006-01-17 | 2009-02-18 | 株式会社日立医药 | Cell culture method and automatic culture system using the method |
| CN200952006Y (en) * | 2006-03-30 | 2007-09-26 | 四川大学 | Oral cavity biological membrane dynamic model device |
| CN201065407Y (en) * | 2007-06-14 | 2008-05-28 | 华中科技大学同济医学院附属同济医院 | In vitro cell open type pressure program control culturing device |
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