CN114729334A - 用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系 - Google Patents

用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系 Download PDF

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Publication number
CN114729334A
CN114729334A CN202080070644.1A CN202080070644A CN114729334A CN 114729334 A CN114729334 A CN 114729334A CN 202080070644 A CN202080070644 A CN 202080070644A CN 114729334 A CN114729334 A CN 114729334A
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China
Prior art keywords
cell
trna synthetase
mutein
trna
amino acid
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CN202080070644.1A
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English (en)
Chinese (zh)
Inventor
詹姆斯·塞巴斯蒂安·依塔利亚
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Brick Biological Co
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Brick Biological Co
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Publication of CN114729334A publication Critical patent/CN114729334A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01004—Leucine--tRNA ligase (6.1.1.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202080070644.1A 2019-08-08 2020-08-07 用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系 Pending CN114729334A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962884465P 2019-08-08 2019-08-08
US201962884454P 2019-08-08 2019-08-08
US62/884,454 2019-08-08
US62/884,465 2019-08-08
US201962936860P 2019-11-18 2019-11-18
US62/936,860 2019-11-18
PCT/US2020/045506 WO2021026506A2 (en) 2019-08-08 2020-08-07 Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids

Publications (1)

Publication Number Publication Date
CN114729334A true CN114729334A (zh) 2022-07-08

Family

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Family Applications (1)

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CN202080070644.1A Pending CN114729334A (zh) 2019-08-08 2020-08-07 用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系

Country Status (9)

Country Link
US (1) US20220325269A1 (enExample)
EP (1) EP4051786A4 (enExample)
JP (1) JP2022545625A (enExample)
KR (1) KR20220097869A (enExample)
CN (1) CN114729334A (enExample)
AU (1) AU2020324460A1 (enExample)
CA (1) CA3150276A1 (enExample)
IL (1) IL290383A (enExample)
WO (1) WO2021026506A2 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220076510A (ko) * 2019-10-08 2022-06-08 트러스티스 오브 보스톤 칼리지 다수의 서로 다른 비천연 아미노산을 함유하는 단백질 및 이러한 단백질의 제조 및 사용 방법
EP4192943A4 (en) * 2020-08-07 2025-02-12 Brickbio, Inc. Stable cell lines for site-specific incorporation of unnatural amino acids
WO2023048291A1 (ja) * 2021-09-24 2023-03-30 富士フイルム株式会社 ｔＲＮＡ、アミノアシルｔＲＮＡ、ポリペプチド合成用試薬、非天然アミノ酸の導入方法、ポリペプチドの作製方法、核酸ディスプレイライブラリの作製方法、核酸－ポリペプチド連結体及びスクリーニング方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134746A1 (en) * 2004-09-21 2006-06-22 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
US20080233611A1 (en) * 2004-10-27 2008-09-25 The Scripps Research Institute Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids
CN101535338A (zh) * 2006-10-18 2009-09-16 斯克利普斯研究院 在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002306849A1 (en) * 2001-03-21 2002-10-08 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
EP4166664A1 (en) * 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
AU2020295571B2 (en) * 2019-06-21 2026-03-26 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134746A1 (en) * 2004-09-21 2006-06-22 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
US20080233611A1 (en) * 2004-10-27 2008-09-25 The Scripps Research Institute Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids
CN101535338A (zh) * 2006-10-18 2009-09-16 斯克利普斯研究院 在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WEI YAN等: "Modulation of Aminoacylation and Editing Properties of Leucyl-tRNA Synthetase by a Conserved Structural Module", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 290, no. 19, pages 12256 *
葛俊驿等: "氨酰tRNA合成酶作用机制及其应用", 中国生物化学与分子生物学报, vol. 33, no. 01, pages 22 - 29 *

Also Published As

Publication number Publication date
EP4051786A2 (en) 2022-09-07
US20220325269A1 (en) 2022-10-13
IL290383A (en) 2022-04-01
WO2021026506A2 (en) 2021-02-11
JP2022545625A (ja) 2022-10-28
CA3150276A1 (en) 2021-02-11
KR20220097869A (ko) 2022-07-08
WO2021026506A3 (en) 2021-03-11
AU2020324460A1 (en) 2022-03-03
EP4051786A4 (en) 2024-04-03

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