CA3150276A1 - Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids - Google Patents

Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids Download PDF

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Publication number
CA3150276A1
CA3150276A1 CA3150276A CA3150276A CA3150276A1 CA 3150276 A1 CA3150276 A1 CA 3150276A1 CA 3150276 A CA3150276 A CA 3150276A CA 3150276 A CA3150276 A CA 3150276A CA 3150276 A1 CA3150276 A1 CA 3150276A1
Authority
CA
Canada
Prior art keywords
cell
trna synthetase
trna
mutein
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3150276A
Other languages
English (en)
French (fr)
Inventor
James Sebastian ITALIA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BrickBio Inc
Original Assignee
BrickBio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BrickBio Inc filed Critical BrickBio Inc
Publication of CA3150276A1 publication Critical patent/CA3150276A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01004—Leucine--tRNA ligase (6.1.1.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3150276A 2019-08-08 2020-08-07 Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids Pending CA3150276A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962884465P 2019-08-08 2019-08-08
US201962884454P 2019-08-08 2019-08-08
US62/884,454 2019-08-08
US62/884,465 2019-08-08
US201962936860P 2019-11-18 2019-11-18
US62/936,860 2019-11-18
PCT/US2020/045506 WO2021026506A2 (en) 2019-08-08 2020-08-07 Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids

Publications (1)

Publication Number Publication Date
CA3150276A1 true CA3150276A1 (en) 2021-02-11

Family

ID=74503664

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3150276A Pending CA3150276A1 (en) 2019-08-08 2020-08-07 Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids

Country Status (9)

Country Link
US (1) US20220325269A1 (enExample)
EP (1) EP4051786A4 (enExample)
JP (1) JP2022545625A (enExample)
KR (1) KR20220097869A (enExample)
CN (1) CN114729334A (enExample)
AU (1) AU2020324460A1 (enExample)
CA (1) CA3150276A1 (enExample)
IL (1) IL290383A (enExample)
WO (1) WO2021026506A2 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220076510A (ko) * 2019-10-08 2022-06-08 트러스티스 오브 보스톤 칼리지 다수의 서로 다른 비천연 아미노산을 함유하는 단백질 및 이러한 단백질의 제조 및 사용 방법
EP4192943A4 (en) * 2020-08-07 2025-02-12 Brickbio, Inc. Stable cell lines for site-specific incorporation of unnatural amino acids
WO2023048291A1 (ja) * 2021-09-24 2023-03-30 富士フイルム株式会社 ｔＲＮＡ、アミノアシルｔＲＮＡ、ポリペプチド合成用試薬、非天然アミノ酸の導入方法、ポリペプチドの作製方法、核酸ディスプレイライブラリの作製方法、核酸－ポリペプチド連結体及びスクリーニング方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002306849A1 (en) * 2001-03-21 2002-10-08 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
US20090181858A1 (en) * 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
CN101048506B (zh) * 2004-10-27 2013-06-26 斯克利普斯研究院 体内掺入非天然氨基酸的正交翻译组分
WO2008073184A2 (en) * 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
EP4166664A1 (en) * 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
AU2020295571B2 (en) * 2019-06-21 2026-03-26 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells

Also Published As

Publication number Publication date
EP4051786A2 (en) 2022-09-07
US20220325269A1 (en) 2022-10-13
IL290383A (en) 2022-04-01
WO2021026506A2 (en) 2021-02-11
JP2022545625A (ja) 2022-10-28
KR20220097869A (ko) 2022-07-08
WO2021026506A3 (en) 2021-03-11
AU2020324460A1 (en) 2022-03-03
CN114729334A (zh) 2022-07-08
EP4051786A4 (en) 2024-04-03

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