IL290383A - Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids - Google Patents
Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acidsInfo
- Publication number
- IL290383A IL290383A IL290383A IL29038322A IL290383A IL 290383 A IL290383 A IL 290383A IL 290383 A IL290383 A IL 290383A IL 29038322 A IL29038322 A IL 29038322A IL 290383 A IL290383 A IL 290383A
- Authority
- IL
- Israel
- Prior art keywords
- aminoacyl
- site
- amino acids
- cell lines
- unnatural amino
- Prior art date
Links
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 title 1
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 title 1
- 150000001413 amino acids Chemical class 0.000 title 1
- 238000010348 incorporation Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
- C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
- C12Y601/01004—Leucine--tRNA ligase (6.1.1.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962884465P | 2019-08-08 | 2019-08-08 | |
| US201962884454P | 2019-08-08 | 2019-08-08 | |
| US201962936860P | 2019-11-18 | 2019-11-18 | |
| PCT/US2020/045506 WO2021026506A2 (en) | 2019-08-08 | 2020-08-07 | Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL290383A true IL290383A (en) | 2022-04-01 |
Family
ID=74503664
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL290383A IL290383A (en) | 2019-08-08 | 2022-02-06 | Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20220325269A1 (enExample) |
| EP (1) | EP4051786A4 (enExample) |
| JP (1) | JP2022545625A (enExample) |
| KR (1) | KR20220097869A (enExample) |
| CN (1) | CN114729334A (enExample) |
| AU (1) | AU2020324460A1 (enExample) |
| CA (1) | CA3150276A1 (enExample) |
| IL (1) | IL290383A (enExample) |
| WO (1) | WO2021026506A2 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20220076510A (ko) * | 2019-10-08 | 2022-06-08 | 트러스티스 오브 보스톤 칼리지 | 다수의 서로 다른 비천연 아미노산을 함유하는 단백질 및 이러한 단백질의 제조 및 사용 방법 |
| EP4192943A4 (en) * | 2020-08-07 | 2025-02-12 | Brickbio, Inc. | Stable cell lines for site-specific incorporation of unnatural amino acids |
| WO2023048291A1 (ja) * | 2021-09-24 | 2023-03-30 | 富士フイルム株式会社 | ｔＲＮＡ、アミノアシルｔＲＮＡ、ポリペプチド合成用試薬、非天然アミノ酸の導入方法、ポリペプチドの作製方法、核酸ディスプレイライブラリの作製方法、核酸－ポリペプチド連結体及びスクリーニング方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002306849A1 (en) * | 2001-03-21 | 2002-10-08 | Elitra Pharmaceuticals, Inc. | Identification of essential genes in microorganisms |
| US20090181858A1 (en) * | 2004-09-21 | 2009-07-16 | The Scripps Research Institute | Adding photoregulated amino acids to the genetic code |
| CN101048506B (zh) * | 2004-10-27 | 2013-06-26 | 斯克利普斯研究院 | 体内掺入非天然氨基酸的正交翻译组分 |
| WO2008073184A2 (en) * | 2006-10-18 | 2008-06-19 | The Scripps Research Institute | Genetic incorporation of unnatural amino acids into proteins in mammalian cells |
| EP4166664A1 (en) * | 2015-03-13 | 2023-04-19 | Chugai Seiyaku Kabushiki Kaisha | Modified aminoacyl-trna synthetase and use thereof |
| AU2020295571B2 (en) * | 2019-06-21 | 2026-03-26 | Trustees Of Boston College | Enhanced platforms for unnatural amino acid incorporation in mammalian cells |
-
2020
- 2020-08-07 US US17/633,672 patent/US20220325269A1/en active Pending
- 2020-08-07 WO PCT/US2020/045506 patent/WO2021026506A2/en not_active Ceased
- 2020-08-07 AU AU2020324460A patent/AU2020324460A1/en not_active Abandoned
- 2020-08-07 CA CA3150276A patent/CA3150276A1/en active Pending
- 2020-08-07 JP JP2022507806A patent/JP2022545625A/ja not_active Ceased
- 2020-08-07 CN CN202080070644.1A patent/CN114729334A/zh active Pending
- 2020-08-07 EP EP20849516.8A patent/EP4051786A4/en active Pending
- 2020-08-07 KR KR1020227007756A patent/KR20220097869A/ko active Pending
-
2022
- 2022-02-06 IL IL290383A patent/IL290383A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP4051786A2 (en) | 2022-09-07 |
| US20220325269A1 (en) | 2022-10-13 |
| WO2021026506A2 (en) | 2021-02-11 |
| JP2022545625A (ja) | 2022-10-28 |
| CA3150276A1 (en) | 2021-02-11 |
| KR20220097869A (ko) | 2022-07-08 |
| WO2021026506A3 (en) | 2021-03-11 |
| AU2020324460A1 (en) | 2022-03-03 |
| CN114729334A (zh) | 2022-07-08 |
| EP4051786A4 (en) | 2024-04-03 |
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