JP2022545625A - 非天然アミノ酸の部位特異的組込みのためのアミノアシルｔｒｎａシンテターゼおよび細胞株 - Google Patents

非天然アミノ酸の部位特異的組込みのためのアミノアシルｔｒｎａシンテターゼおよび細胞株 Download PDF

Info

Publication number
JP2022545625A
JP2022545625A JP2022507806A JP2022507806A JP2022545625A JP 2022545625 A JP2022545625 A JP 2022545625A JP 2022507806 A JP2022507806 A JP 2022507806A JP 2022507806 A JP2022507806 A JP 2022507806A JP 2022545625 A JP2022545625 A JP 2022545625A
Authority
JP
Japan
Prior art keywords
cell
trna
trna synthetase
mutein
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP2022507806A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022545625A5 (enExample
Inventor
イタリア，ジェームズ，セバスチャン
Original Assignee
ブリックバイオ，インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ブリックバイオ，インコーポレイテッド filed Critical ブリックバイオ，インコーポレイテッド
Publication of JP2022545625A publication Critical patent/JP2022545625A/ja
Publication of JP2022545625A5 publication Critical patent/JP2022545625A5/ja
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01004—Leucine--tRNA ligase (6.1.1.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2022507806A 2019-08-08 2020-08-07 非天然アミノ酸の部位特異的組込みのためのアミノアシルｔｒｎａシンテターゼおよび細胞株 Ceased JP2022545625A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962884465P 2019-08-08 2019-08-08
US201962884454P 2019-08-08 2019-08-08
US62/884,454 2019-08-08
US62/884,465 2019-08-08
US201962936860P 2019-11-18 2019-11-18
US62/936,860 2019-11-18
PCT/US2020/045506 WO2021026506A2 (en) 2019-08-08 2020-08-07 Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids

Publications (2)

Publication Number Publication Date
JP2022545625A true JP2022545625A (ja) 2022-10-28
JP2022545625A5 JP2022545625A5 (enExample) 2023-08-23

Family

ID=74503664

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022507806A Ceased JP2022545625A (ja) 2019-08-08 2020-08-07 非天然アミノ酸の部位特異的組込みのためのアミノアシルｔｒｎａシンテターゼおよび細胞株

Country Status (9)

Country Link
US (1) US20220325269A1 (enExample)
EP (1) EP4051786A4 (enExample)
JP (1) JP2022545625A (enExample)
KR (1) KR20220097869A (enExample)
CN (1) CN114729334A (enExample)
AU (1) AU2020324460A1 (enExample)
CA (1) CA3150276A1 (enExample)
IL (1) IL290383A (enExample)
WO (1) WO2021026506A2 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220076510A (ko) * 2019-10-08 2022-06-08 트러스티스 오브 보스톤 칼리지 다수의 서로 다른 비천연 아미노산을 함유하는 단백질 및 이러한 단백질의 제조 및 사용 방법
EP4192943A4 (en) * 2020-08-07 2025-02-12 Brickbio, Inc. Stable cell lines for site-specific incorporation of unnatural amino acids
WO2023048291A1 (ja) * 2021-09-24 2023-03-30 富士フイルム株式会社 ｔＲＮＡ、アミノアシルｔＲＮＡ、ポリペプチド合成用試薬、非天然アミノ酸の導入方法、ポリペプチドの作製方法、核酸ディスプレイライブラリの作製方法、核酸－ポリペプチド連結体及びスクリーニング方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008073184A2 (en) * 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
JP2012254073A (ja) * 2004-10-27 2012-12-27 Scripps Research Inst 非天然アミノ酸のインビボ組込み用直交翻訳成分

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002306849A1 (en) * 2001-03-21 2002-10-08 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
US20090181858A1 (en) * 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
EP4166664A1 (en) * 2015-03-13 2023-04-19 Chugai Seiyaku Kabushiki Kaisha Modified aminoacyl-trna synthetase and use thereof
AU2020295571B2 (en) * 2019-06-21 2026-03-26 Trustees Of Boston College Enhanced platforms for unnatural amino acid incorporation in mammalian cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012254073A (ja) * 2004-10-27 2012-12-27 Scripps Research Inst 非天然アミノ酸のインビボ組込み用直交翻訳成分
WO2008073184A2 (en) * 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGEW. CHEM. INT. ED., vol. 49, JPN6025021840, 2010, pages 935 - 937, ISSN: 0005606984 *
BIOCHEMISTRY, vol. 57, JPN6024027116, 2018, pages 441 - 445, ISSN: 0005489214 *
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 18, JPN6024027117, 2008, pages 6004 - 6006, ISSN: 0005489213 *
PNAS, vol. 103, no. 26, JPN6024027115, 2006, pages 9785 - 9789, ISSN: 0005489215 *

Also Published As

Publication number Publication date
EP4051786A2 (en) 2022-09-07
US20220325269A1 (en) 2022-10-13
IL290383A (en) 2022-04-01
WO2021026506A2 (en) 2021-02-11
CA3150276A1 (en) 2021-02-11
KR20220097869A (ko) 2022-07-08
WO2021026506A3 (en) 2021-03-11
AU2020324460A1 (en) 2022-03-03
CN114729334A (zh) 2022-07-08
EP4051786A4 (en) 2024-04-03

Similar Documents

Publication Publication Date Title
JP7640538B2 (ja) 複数の異なる非天然アミノ酸を含有するタンパク質、並びにそのようなタンパク質の製造方法及び使用方法
JP7474511B2 (ja) ＡＣＥ－ｔＲＮＡを用いた遺伝的再帰属を介して終止コドンレスキューする方法
CN104603272B (zh) 能够产生腺伴随病毒载体的细胞
US20220325269A1 (en) Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
KR20240025507A (ko) 미성숙 종결 코돈-매개 장애를 치료하기 위한 방법 및 조성물
JP2025098162A (ja) 哺乳動物細胞における非天然アミノ酸の組み込みのための強化されたプラットフォーム
JP2023524401A (ja) 遺伝子治療の増強のためのアデノ随伴ウイルス（ａａｖ）の制御された修飾
US20230313129A1 (en) Stable cell lines for site-specific incorporation of unnatural amino acids
JP2025020287A (ja) タンパク質製造用ベクター
CN113727735A (zh) 启动子序列和相关产物及其用途
WO2024003718A1 (en) Methods and kits for the improved fermentative production of a recombinant virus
JP2025519357A (ja) プロモーター活性を有する核酸およびその使用

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220328

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230802

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230802

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240709

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241009

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20241223

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20250321

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250414

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250530

A045 Written measure of dismissal of application [lapsed due to lack of payment]

Free format text: JAPANESE INTERMEDIATE CODE: A045

Effective date: 20250926