CN114703193A - 多适配子靶标检测 - Google Patents

多适配子靶标检测 Download PDF

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Publication number
CN114703193A
CN114703193A CN202210264586.7A CN202210264586A CN114703193A CN 114703193 A CN114703193 A CN 114703193A CN 202210264586 A CN202210264586 A CN 202210264586A CN 114703193 A CN114703193 A CN 114703193A
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China
Prior art keywords
aptamer
deoxyuridine
carboxamide
target
complex
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Pending
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CN202210264586.7A
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English (en)
Chinese (zh)
Inventor
U·A·奥克斯纳
L·S·格林
L·戈尔德
N·亚尼奇
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Private Placement Protein Body Operation Co ltd
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Private Placement Protein Body Operation Co ltd
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Publication of CN114703193A publication Critical patent/CN114703193A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Saccharide Compounds (AREA)
CN202210264586.7A 2013-09-24 2014-09-24 多适配子靶标检测 Pending CN114703193A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361881629P 2013-09-24 2013-09-24
US61/881,629 2013-09-24
CN201480051210.1A CN105555957A (zh) 2013-09-24 2014-09-24 多适配子靶标检测
PCT/US2014/057143 WO2015048084A1 (en) 2013-09-24 2014-09-24 Multiaptamer target detection

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201480051210.1A Division CN105555957A (zh) 2013-09-24 2014-09-24 多适配子靶标检测

Publications (1)

Publication Number Publication Date
CN114703193A true CN114703193A (zh) 2022-07-05

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
CN202210264586.7A Pending CN114703193A (zh) 2013-09-24 2014-09-24 多适配子靶标检测
CN201480051210.1A Pending CN105555957A (zh) 2013-09-24 2014-09-24 多适配子靶标检测

Family Applications After (1)

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CN201480051210.1A Pending CN105555957A (zh) 2013-09-24 2014-09-24 多适配子靶标检测

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US (2) US9926566B2 (enExample)
EP (1) EP3049523B1 (enExample)
JP (1) JP6506768B2 (enExample)
KR (1) KR102348283B1 (enExample)
CN (2) CN114703193A (enExample)
AU (1) AU2014326975B2 (enExample)
BR (1) BR112016005957A2 (enExample)
CA (1) CA2924987C (enExample)
HK (1) HK1218765A1 (enExample)
IL (1) IL244284B (enExample)
MX (1) MX361451B (enExample)
SG (1) SG11201602063UA (enExample)
WO (1) WO2015048084A1 (enExample)

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JP2019521680A (ja) * 2016-06-17 2019-08-08 プロクシミティー バイオサイエンシズ，エルエルシー アプタマーペアを選択する方法
EP3913058B1 (en) 2016-07-01 2025-01-01 SomaLogic Operating Co., Inc. Oligonucleotides comprising modified nucleosides
EP3548652B1 (en) 2016-12-01 2024-04-03 Nautilus Subsidiary, Inc. Methods of assaying proteins
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
JP7526102B2 (ja) * 2018-06-22 2024-07-31 ソマロジック・オペレイティング・カンパニー・インコーポレイテッド 改良されたプロテオミクスマルチプレックスアッセイ
EP4165549A1 (en) 2020-06-11 2023-04-19 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
WO2022159663A1 (en) 2021-01-21 2022-07-28 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
EP4399528A1 (en) 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
US20230090454A1 (en) 2021-09-22 2023-03-23 Nautilus Biotechnology, Inc. Methods and systems for determining polypeptide interactions
US20230149883A1 (en) 2021-11-03 2023-05-18 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
WO2023192917A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
US20240094215A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
EP4619766A1 (en) 2022-11-15 2025-09-24 Nautilus Subsidiary, Inc. Standard polypeptides
EP4630816A1 (en) 2022-12-09 2025-10-15 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
US20240201182A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
WO2024206122A1 (en) 2023-03-24 2024-10-03 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024216233A1 (en) 2023-04-13 2024-10-17 Nautilus Subsidiary, Inc. Artificial proteins for displaying epitopes
WO2024243237A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Citations (3)

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CN101802225A (zh) * 2007-07-17 2010-08-11 私募蛋白质体公司 检测样品的多元分析
US20120231467A1 (en) * 2011-03-10 2012-09-13 Somalogic, Inc. Aptamers for C. Difficile Diagnostics
CN102858990A (zh) * 2010-04-12 2013-01-02 私募蛋白质体公司 针对β-NGF的适体及其在治疗β-NGF介导的疾病和失调中的用途

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CN102858990A (zh) * 2010-04-12 2013-01-02 私募蛋白质体公司 针对β-NGF的适体及其在治疗β-NGF介导的疾病和失调中的用途
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Publication number Publication date
CA2924987C (en) 2022-08-16
IL244284A0 (en) 2016-04-21
AU2014326975B2 (en) 2020-05-07
JP2016531593A (ja) 2016-10-13
WO2015048084A1 (en) 2015-04-02
KR20160058777A (ko) 2016-05-25
KR102348283B1 (ko) 2022-01-10
US10392621B2 (en) 2019-08-27
EP3049523A1 (en) 2016-08-03
JP6506768B2 (ja) 2019-04-24
US20180187197A1 (en) 2018-07-05
IL244284B (en) 2019-12-31
CN105555957A (zh) 2016-05-04
MX361451B (es) 2018-12-06
SG11201602063UA (en) 2016-04-28
AU2014326975A2 (en) 2017-01-05
EP3049523A4 (en) 2017-05-17
EP3049523B1 (en) 2019-08-07
US20160237435A1 (en) 2016-08-18
HK1218765A1 (zh) 2017-03-10
CA2924987A1 (en) 2015-04-02
MX2016003320A (es) 2016-05-31
BR112016005957A2 (pt) 2017-09-26
US9926566B2 (en) 2018-03-27
AU2014326975A1 (en) 2016-04-07

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