CN1146489A - 微生物的灭活、破坏方法 - Google Patents
微生物的灭活、破坏方法 Download PDFInfo
- Publication number
- CN1146489A CN1146489A CN95119096A CN95119096A CN1146489A CN 1146489 A CN1146489 A CN 1146489A CN 95119096 A CN95119096 A CN 95119096A CN 95119096 A CN95119096 A CN 95119096A CN 1146489 A CN1146489 A CN 1146489A
- Authority
- CN
- China
- Prior art keywords
- microorganism
- electric energy
- deactivation
- cells
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 230000002427 irreversible effect Effects 0.000 claims abstract description 10
- 239000007787 solid Substances 0.000 claims abstract description 10
- 239000007789 gas Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 41
- 230000006378 damage Effects 0.000 claims description 21
- 230000009849 deactivation Effects 0.000 claims description 17
- 230000003068 static effect Effects 0.000 claims description 8
- 238000005868 electrolysis reaction Methods 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 230000001590 oxidative effect Effects 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 14
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 13
- 230000001954 sterilising effect Effects 0.000 description 9
- 239000008213 purified water Substances 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 5
- 241000222122 Candida albicans Species 0.000 description 4
- 206010007134 Candida infections Diseases 0.000 description 4
- 241000606768 Haemophilus influenzae Species 0.000 description 4
- 241000191967 Staphylococcus aureus Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 201000003984 candidiasis Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000000428 dust Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000005686 electrostatic field Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 229960001005 tuberculin Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/03—Electric current
- A61L2/035—Electrolysis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/467—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis by electrochemical disinfection; by electrooxydation or by electroreduction
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/48—Treatment of water, waste water, or sewage with magnetic or electric fields
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/461—Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
- C02F1/46104—Devices therefor; Their operating or servicing
- C02F1/4618—Devices therefor; Their operating or servicing for producing "ionised" acidic or basic water
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Electrochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本发明提供了一种对微生物的更有效的灭活、破坏方法，本发明通过具有电能的液体、气体或固体给微生物以电能，微生物所具电荷增加，而超过微生物的细胞内外的静电容限度，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜破裂。
Description
本发明涉及旨在杀菌、杀病毒、消毒或灭菌的微生物灭活、破坏方法。
目前已提出各种方法，可高效地破坏包括绿脓杆菌、大肠杆菌等细菌及真菌或病毒等的微生物，进行杀菌、消毒、灭菌处理。然而，可以认为，这些方法皆未对微生物的特性作充分研究。例如，人们已知其中有电化学杀菌方法，但该方法的使用却以下述事实为前提，即，使微生物接触电极表面或电介质，阻碍其生化反应而破坏微生物。细菌通常在其表面带有负电荷，为阳极所吸引，接触，从而发生菌体的破坏，这是事实。但是，如菌体量增多，菌体内部的原生质(蛋白液)会蓄积，而出现蛋白质保护了细菌的状态，这是由于形成某种电屏蔽状态，导致杀菌功能降低，也易产生电极维修保养的问题，这样，其实用化也被认为有困难。
然而，本发明者们注意到细菌的破坏状况，考虑到微生物的特性，就微生物是以何种机制被破坏的作了种种研究。其结果，确认了这一现象：即细菌在接触阳极之前，就在喷出细胞质的同时发生崩溃的。而且，根据以上的认识，又了解到，作为微生物电容器的菌体，当其在静电场中接受的电荷超过其静电容时，则发生细胞膜的破坏，菌体死亡。本发明即基于这样的见解而提出。
与本发明有关的微生物的灭活、破坏方法，其主要在于，第一，通过具有电能的液体、气体或固体给微生物以电能；第二，由此，使其电荷量增加(上升)，而超过微生物所具有的细胞内外的静电容的限度，及/或促进微生物细胞内外的渗透性；第三，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜发生破裂。
本发明中的具有电能的液体最好使用带电荷的水，微生物处于该带电荷的水溶液中给以电能。上述带电荷的水可以是带静电的水(荷电水)，蓄电水、功能水、电解处理水、强氧化电位水、强酸性电解生成的水溶液、电离水、非电离水或带电水中之任一种。又，具有电能的气体指带有电荷的气体，具有电能的固体指蓄电体。
另外，也可使用电极。此时，微生物的灭活、破坏方法主要在于：第一，通过电极对含有微生物的液体通电，对处于与电极非接触状态的微生物给予电能；第二，使所带电荷上升，超过微生物所具有的细胞内外的静电容限度，及/或促进微生物细胞内外的渗透性；第三，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜发生破坏。另外，通过电极通电可以是电介性通电，也可以是非电介性通电。
在以上或以下的说明中，“微生物”用作包括真菌的细菌或病毒等的总称。又，“界面膜”在广义上，用作对从外界遮蔽细胞的原生质的界面膜、外膜、原生质膜、细胞壁等的总称。另外，“破裂”具有广义，直接意指在细胞膜的弹性很强时，膜发生强烈收缩，其内容物(原生质)呈辐射状向多方面散乱喷出的状态；或在细胞膜的弹性较小，且细胞的内压很高时，(细胞膜)发生局部破坏，(内容物)发生平行喷出，周围无散乱的状态；或在细胞膜的弹性较小，而外压相对较低时，同时发生平行喷出和周围散乱的状态等；但是，“破裂”并不限于这些，间接地包括胀压破坏，可广义地包括作为菌体破坏的一般现象的溶解、脱水、凝固、融解、穿透等的破坏状态。
再有，“带电荷的水”用作荷电水、蓄电水、功能水、电解处理水、强氧化电位水、强酸性电解生成的水溶液、电离水、非电离水或带电水等的总称。又，“通电”不限于加了电解质(NaCl及其它)那样的溶液的电介性通电，也包含对于如纯水或精制水等一般被认为无导电性的溶液的非电介性通电。而且，在后一场合，本发明得们确认，使活菌浮游于纯水或精制水中通电时，可得到比电介性通电为小的电流。已阐明，这是由于存在于菌体间的一种跳跃传导作用(非电介性通电的一种)，菌体形成了流向阳极方向的恒定流动。
而且，“渗透”(osmosis)意指由于可透过水、但不能透过溶质的分隔，溶液中的水移向浓度高的一侧的现象，如渗透压升高则与界面膜的破坏有关。再有，“不可逆的变化”用于表于阻碍界面膜的物质交换的状态，及导致界面膜自身的变性、变质或原生质的变性、变质的状态的变化的总称。
发明实施形式第一实施形式
选金黄色葡萄球菌、大肠杆菌、绿脓杆菌、念珠菌及流感嗜血杆菌作为微生物，使分别浮游于预先以微量加至多个试样上的0.01ml的精制水或自来水中，然后，以结核菌素注射器逐滴滴下强氧化电位水，组合使用附有微分差干涉装置的倒置型系统显微镜IX-70(奥林巴斯公司制)，配合14英寸监控器(附CCD)，使所用装置最终放大倍数达4500倍，对微生物作立体层状的灭活、破坏状态的检查。
强氧化电位水的每一滴为0.01ml，逐滴滴下，作五阶段观察。可以看到，上述微生物中之任一种都在滴下1滴时有70％、2滴时有80％、3滴时有99％的微生物细胞发生破裂形成碎屑。滴入4滴时碎屑增加，最后滴入5滴时，微生物细胞几近100％呈现崩溃状态。
绿脓杆菌比起其它的微生物来，为较强的细菌，但是，值得注意的是，其微生物细胞与上述其它菌同样崩溃。然而，在念珠菌的场合，大概由于其本身为不动的真菌，难以看到界面破坏，但几乎所有的菌体都停止摇动，仍可确认念球菌失活或者破坏。另外，在滴下3滴时，菌体虽未破裂，但有处于不动状态的菌体，而非常罕见地，具有微小的浮游性的菌体。第二实施形式
准备好多个试样，分别在其上以微量供给0.01ml的精制水或0.01ml的自来水，分别选用金黄色葡萄球菌、大肠杆菌、绿脓杆菌、念珠菌及流感嗜血杆菌作为微生物，使其浮游。再分别通过铂电极，对精制水给予0.2mA-0.3mA：12V的电能，对自来水给予0.5mA：12V的电能，可看到99％的菌体细胞膜发生破裂。第三实施形式
使用由等离子体放电产生的电离性气体作为具有电能的气体。在多个试样上微量供给0.01ml的精制水，分别选用金黄色葡萄球菌、大肠杆菌、绿脓杆菌、念珠菌及流感嗜血杆菌作为微生物，使其分别浮游。将放电管置于上述微生物附近，使放电管的外周产生的电离性气体发生作用。可以看到，99％的菌体的细胞膜发生破裂。由此可确认，对微生物给予电能，既使微生物处于非接触状态，也可对该微生物进行所要求的灭活和破坏。第四实施形式
使用带电的碳粉作为具有电能的固体。在多个试样上微量供给0.01ml的精制水，选用金黄色葡萄球菌、大肠杆菌、绿脓杆菌、念珠菌及流感嗜血杆菌作微生物，使其各浮游水中。再对其撒以微量碳粉，可看到，同样有99％的菌体的细胞膜破裂。另外，同样使用带电的聚合物粉末，作为具有电能的固体，取代碳粉，可得到同样的结果。第五实施形式
对可在湿润后培养的干燥活菌，施加以带电碳粉，结果，该干燥活菌的培养度极低。
发明的效果
根据本发明，给予了电能的细胞，因其界面膜破裂，具有杀菌、杀病毒、消毒的效果，且灭菌效果非常高。另外的优良效果是，给予微生物的电能，只要是具有电能，液体、气体或固体都可使用，因此，可按欲灭活、杀死的微生物浮游或存在的部位、局部、场所，适当选用非常广的范围的电能给予方法。
Claims (11)
1.一种微生物的灭活、破坏方法，其特征在于，通过具有电能的液体、气体或固体，给微生物以电能，由此使其电荷量增加，而超过微生物所具有的细胞内外的静电容限度，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜发生破裂。
2.一种微生物的灭活、破坏方法，其特征在于，通过具有电能的液体、气体或固体，给微生物以电能，促进微生物细胞内外的渗透性，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜发生破裂。
3.一种微生物的灭活、破坏方法，其特征在于，通过具有电能的液体、气体或固体，给微生物以电能，由此，使基电荷量增加，而超过微生物所具有的细胞内外的静电容限度，且促进微生物细胞内外的渗透性，从而使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜破裂。
4.如权利要求1-3中之任一项所述的微生物的灭活、破坏方法，其特征在于，具有电能的液体为带电荷的水，使微生物处于该带电水溶液中而给予电能。
5.如权利要求4所述的微生物的灭活、破坏方法，其特征在于，所述带电荷的水为荷电水、蓄电水、功能水、电解处理水、强氧化电位水、强酸性电解生成的水溶液、电离水、非电离水或带电水。
6.如权利要求1-3之任一项所述的微生物的灭活、破坏方法，其特征在于，具有电能的气体为带有电荷的气体。
7.如权利要求1-3之任一项所述的微生物的灭活、破坏方法，其特征在于，具有电能的固体为蓄电体。
8.一种微生物微生物的灭活、破坏方法，其特征在于，通过电极对含有微生物的液体通电，对与电极处于非接触状态的微生物给予电能，使其电荷增加，超过微生物所具有的细胞内外的静电容限度，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜发生破裂。
9.一种微生物的灭活、破坏方法，其特征在于，通过电极对含有微生物的液体通电，对处于与电极非接触状态的微生物给予电能，促进微生物细胞内外的渗透性，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜发生破裂。
10.一种微生物的灭活、破坏方法，其特征在于，通过电极对含有微生物的液体通电，对处于与电极非接触状态的微生物给予电能，使其电荷量增加，超过该微生物所具有的细胞内外的静电容限度，且促进微生物细胞内外的渗透性，使微生物细胞发生不可逆的变化，及/或使微生物细胞的界面膜破裂。
11.如权利要求8-10之任一项所述的的微生物的灭活、破坏方法，其特征在于，所述通电为电介性通电或非电介性通电。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7251623A JPH0994288A (ja) | 1995-09-28 | 1995-09-28 | 微生物の不活化・破壊方法 |
| JP251623/95 | 1995-09-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1146489A true CN1146489A (zh) | 1997-04-02 |
Family
ID=17225587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95119096A Pending CN1146489A (zh) | 1995-09-28 | 1995-12-28 | 微生物的灭活、破坏方法 |
Country Status (23)
| Country | Link |
|---|---|
| US (1) | US5922209A (zh) |
| EP (1) | EP0765843A1 (zh) |
| JP (1) | JPH0994288A (zh) |
| KR (1) | KR970015473A (zh) |
| CN (1) | CN1146489A (zh) |
| AR (1) | AR000805A1 (zh) |
| AU (1) | AU694292B2 (zh) |
| BR (1) | BR9600285A (zh) |
| CA (1) | CA2166855C (zh) |
| CZ (1) | CZ30996A3 (zh) |
| FI (1) | FI960193A (zh) |
| HU (1) | HUP9602683A2 (zh) |
| IL (1) | IL116812A0 (zh) |
| MA (1) | MA23790A1 (zh) |
| MX (1) | MX9600439A (zh) |
| NO (1) | NO960242L (zh) |
| NZ (1) | NZ280785A (zh) |
| PE (1) | PE26297A1 (zh) |
| PL (1) | PL313858A1 (zh) |
| RU (1) | RU2108113C1 (zh) |
| SG (1) | SG48713A1 (zh) |
| TW (1) | TW393446B (zh) |
| ZA (1) | ZA96407B (zh) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104058561A (zh) * | 2013-03-22 | 2014-09-24 | 上海易昆机械工程有限公司 | 一种细胞破解器 |
| CN109908035A (zh) * | 2019-04-25 | 2019-06-21 | 山西纳安生物科技有限公司 | 一种基于牡丹籽油的祛斑面膜 |
| WO2019218754A1 (zh) * | 2018-05-14 | 2019-11-21 | 深圳市中科摩方科技有限公司 | 一种表面超电容修饰的材料及其制备方法和应用 |
| CN112556078A (zh) * | 2020-11-11 | 2021-03-26 | 山东青洁能环保有限公司 | 感应正静电超低容量雾化电位水消毒除臭方法及设备 |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2763957B1 (fr) * | 1997-05-29 | 2001-09-14 | Bio Merieux | Procede pour isoler un materiel intracellulaire et procede de traitement d'un materiel nucleique |
| AU2003206513A1 (en) * | 2002-02-12 | 2003-09-04 | Les Technologies Elcotech Inc. | Method for the treatment of slurries by the combined action of pressure and electro-osmosis |
| WO2004050562A1 (fr) * | 2002-11-29 | 2004-06-17 | Les Technologies Elcotech Inc. | Appareil et procédé de traitement des eaux usées par electroflottation et/ou electrocoagulation |
| CA2437245A1 (fr) * | 2003-08-11 | 2005-02-11 | Les Technologies Elcotech Inc. | Appareil permettant le traitement des boues a forte siccitee |
| US20100276294A1 (en) * | 2003-03-28 | 2010-11-04 | Lambie John M | Electrolytic sanitization of water |
| NO327633B1 (no) * | 2003-11-04 | 2009-09-07 | Environmental Solutions As | Fremgangsmate, anlegg samt komponent behandling av ballastvann. |
| US7959780B2 (en) | 2004-07-26 | 2011-06-14 | Emporia Capital Funding Llc | Textured ion exchange membranes |
| US7780833B2 (en) | 2005-07-26 | 2010-08-24 | John Hawkins | Electrochemical ion exchange with textured membranes and cartridge |
| WO2007044609A1 (en) | 2005-10-06 | 2007-04-19 | Pionetics Corporation | Electrochemical ion exchange treatment of fluids |
| US20100072059A1 (en) * | 2008-09-25 | 2010-03-25 | Peters Michael J | Electrolytic System and Method for Enhanced Radiological, Nuclear, and Industrial Decontamination |
| US20140295038A1 (en) | 2013-03-27 | 2014-10-02 | Bnr Technology Development, Llc | Method for extending the shelf life of liquid comestibles |
| US9757695B2 (en) | 2015-01-03 | 2017-09-12 | Pionetics Corporation | Anti-scale electrochemical apparatus with water-splitting ion exchange membrane |
| US11191977B1 (en) | 2020-11-12 | 2021-12-07 | Howard Letovsky | Applications of bioactive frequencies and menus thereof |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3366564A (en) * | 1965-02-02 | 1968-01-30 | Gen Electric | Electrohydraulic process |
| US3943044A (en) * | 1971-09-07 | 1976-03-09 | Diamond Shamrock Corporation | Method for treating sewage water |
| DE2246955C3 (de) * | 1972-09-25 | 1980-10-02 | Krautkraemer, Gmbh, 5000 Koeln | Ultraschall-Meßverfahren zur Bestimmung von Wanddicken |
| US3925176A (en) * | 1973-10-10 | 1975-12-09 | Adolph P Okert | Apparatus and method for electrolytic sewage treatment |
| US4012310A (en) * | 1975-09-11 | 1977-03-15 | Progressive Equipment Corporation | Electrostatic water treatment system |
| US4305390A (en) * | 1975-11-28 | 1981-12-15 | Massachusetts Institute Of Technology | Method for generating oxygen in an excited electronic state and inactivation of microorganisms |
| DE2643213C2 (de) * | 1976-09-25 | 1985-02-21 | Bayer Ag, 5090 Leverkusen | Verfahren zum Abschwächen oder Inaktivieren von Mikroorganismen |
| US4179347A (en) * | 1978-02-28 | 1979-12-18 | Omnipure, Inc. | System for electrocatalytic treatment of waste water streams |
| DE2815030A1 (de) * | 1978-04-07 | 1979-10-18 | Hoechst Ag | Verfahren zur anreicherung von biomasse |
| JPS5939143B2 (ja) * | 1981-12-12 | 1984-09-21 | 株式会社巴商会 | プラズマ滅菌法 |
| DE3233282A1 (de) * | 1982-09-08 | 1984-03-08 | Friedhelm Dr.-Ing. 5300 Bonn Caspers | Keiminaktivierung ueber elektromagnetische hochfrequenzenergie |
| JPS6176160A (ja) * | 1984-09-21 | 1986-04-18 | 松永 是 | 殺細胞方法 |
| US5048404A (en) * | 1985-05-31 | 1991-09-17 | Foodco Corporation | High pulsed voltage systems for extending the shelf life of pumpable food products |
| JPS6382666A (ja) * | 1986-09-26 | 1988-04-13 | 三菱重工業株式会社 | 高電圧パルスによる殺菌装置 |
| JPS647964A (en) * | 1987-06-30 | 1989-01-11 | Toshiba Corp | Air cleaner and air conditioner equipped with this cleaner |
| US4970154A (en) * | 1987-10-09 | 1990-11-13 | Baylor College Of Medicine | Method for inserting foreign genes into cells using pulsed radiofrequency |
| US5091152A (en) * | 1988-05-19 | 1992-02-25 | Thomas Sr Tim L | Apparatus for electrically destroying targeted organisms in fluids |
| JPH078768B2 (ja) * | 1988-06-06 | 1995-02-01 | ジプコム株式会社 | 殺菌水 |
| JPH02172467A (ja) * | 1988-12-27 | 1990-07-04 | Amosu:Kk | 抗菌性セラミックス材料とその製造および使用方法 |
| JP2651740B2 (ja) * | 1990-07-26 | 1997-09-10 | ハウス食品株式会社 | 高電圧パルスによる殺菌方法 |
| US5308507A (en) * | 1992-08-04 | 1994-05-03 | Los Alamos Technical Associates, Inc. | Method and apparatus for removing organic contaminants |
| JP2649133B2 (ja) * | 1993-02-15 | 1997-09-03 | 賢士 宍戸 | レジオネラ属菌殺菌除去用濾過装置 |
| NZ264188A (en) * | 1994-08-05 | 1996-10-28 | Ennotech Holdings Ltd | Method and apparatus for sterilising electrically conductive liquid by creating a voltage gradient between electrodes in contact with the liquid |
| US5635059A (en) * | 1994-10-20 | 1997-06-03 | Aqua-Ion Systems, Inc. | Method and apparatus for water treatment and purification using gas ion plasma source and disinfectant metal ion complexes |
| US5695650A (en) * | 1995-11-02 | 1997-12-09 | Held; Jeffery S. | Method for dewatering previously-dewatered municipal waste-water sludges using high electrical voltage |
-
1995
- 1995-09-28 JP JP7251623A patent/JPH0994288A/ja active Pending
- 1995-12-28 CN CN95119096A patent/CN1146489A/zh active Pending
- 1995-12-29 KR KR1019950066743A patent/KR970015473A/ko not_active Application Discontinuation
- 1995-12-29 TW TW084114140A patent/TW393446B/zh active
- 1995-12-29 EP EP95309545A patent/EP0765843A1/en not_active Withdrawn
-
1996
- 1996-01-04 NZ NZ280785A patent/NZ280785A/en unknown
- 1996-01-04 AU AU40830/96A patent/AU694292B2/en not_active Ceased
- 1996-01-09 CA CA002166855A patent/CA2166855C/en not_active Expired - Fee Related
- 1996-01-15 FI FI960193A patent/FI960193A/fi not_active Application Discontinuation
- 1996-01-18 IL IL11681296A patent/IL116812A0/xx unknown
- 1996-01-18 ZA ZA96407A patent/ZA96407B/xx unknown
- 1996-01-19 SG SG1996000303A patent/SG48713A1/en unknown
- 1996-01-19 NO NO960242A patent/NO960242L/no unknown
- 1996-01-23 PE PE1996000053A patent/PE26297A1/es not_active Application Discontinuation
- 1996-01-25 AR ARP960101153A patent/AR000805A1/es unknown
- 1996-01-31 MX MX9600439A patent/MX9600439A/es unknown
- 1996-01-31 MA MA24145A patent/MA23790A1/fr unknown
- 1996-01-31 BR BR9600285A patent/BR9600285A/pt not_active Application Discontinuation
- 1996-02-02 CZ CZ96309A patent/CZ30996A3/cs unknown
- 1996-02-14 RU RU96102855A patent/RU2108113C1/ru active
- 1996-04-19 PL PL96313858A patent/PL313858A1/xx unknown
- 1996-09-27 HU HU9602683A patent/HUP9602683A2/hu unknown
-
1997
- 1997-11-12 US US08/967,887 patent/US5922209A/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104058561A (zh) * | 2013-03-22 | 2014-09-24 | 上海易昆机械工程有限公司 | 一种细胞破解器 |
| WO2019218754A1 (zh) * | 2018-05-14 | 2019-11-21 | 深圳市中科摩方科技有限公司 | 一种表面超电容修饰的材料及其制备方法和应用 |
| CN110896607A (zh) * | 2018-05-14 | 2020-03-20 | 深圳市中科摩方科技有限公司 | 一种表面超电容修饰的材料及其制备方法和应用 |
| CN109908035A (zh) * | 2019-04-25 | 2019-06-21 | 山西纳安生物科技有限公司 | 一种基于牡丹籽油的祛斑面膜 |
| CN112556078A (zh) * | 2020-11-11 | 2021-03-26 | 山东青洁能环保有限公司 | 感应正静电超低容量雾化电位水消毒除臭方法及设备 |
Also Published As
| Publication number | Publication date |
|---|---|
| MX9600439A (es) | 1997-03-29 |
| FI960193A0 (fi) | 1996-01-15 |
| PL313858A1 (en) | 1997-04-01 |
| MA23790A1 (fr) | 1996-10-01 |
| NO960242L (no) | 1997-04-01 |
| IL116812A0 (en) | 1996-05-14 |
| AU694292B2 (en) | 1998-07-16 |
| SG48713A1 (en) | 1998-05-18 |
| EP0765843A1 (en) | 1997-04-02 |
| ZA96407B (en) | 1997-01-20 |
| NO960242D0 (no) | 1996-01-19 |
| AR000805A1 (es) | 1997-08-06 |
| BR9600285A (pt) | 1997-12-23 |
| NZ280785A (en) | 1997-11-24 |
| JPH0994288A (ja) | 1997-04-08 |
| FI960193A (fi) | 1997-03-29 |
| CA2166855C (en) | 1999-01-19 |
| PE26297A1 (es) | 1997-08-14 |
| HU9602683D0 (en) | 1996-11-28 |
| TW393446B (en) | 2000-06-11 |
| RU2108113C1 (ru) | 1998-04-10 |
| HUP9602683A2 (en) | 1997-06-30 |
| CZ30996A3 (cs) | 1998-03-18 |
| CA2166855A1 (en) | 1997-03-29 |
| AU4083096A (en) | 1997-04-10 |
| KR970015473A (ko) | 1997-04-28 |
| US5922209A (en) | 1999-07-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1146489A (zh) | 微生物的灭活、破坏方法 | |
| US7704353B2 (en) | Electrode assembly for the electrochemical treatment of liquids with a low conductivity | |
| US5091152A (en) | Apparatus for electrically destroying targeted organisms in fluids | |
| CN102600726B (zh) | 离子性物质去除系统 | |
| US20080156642A1 (en) | System for the Disinfection of Low-Conductivity Liquids | |
| KR101015621B1 (ko) | 순간 살균 소독수 생성장치 | |
| CN103932358A (zh) | 果蔬清洗机 | |
| CN1329576A (zh) | 用于纯化水溶液及合成化学品的电解装置、方法 | |
| CN103459328A (zh) | 水的净化 | |
| JP2009190016A (ja) | 水浄化処理用電解キャパシター内のバイオフィルム除去及び防止方法 | |
| CN107735366B (zh) | 用于去除水中杂质的电吸附系统 | |
| US6798639B2 (en) | Fluid deionization flow through capacitor systems | |
| KR100621937B1 (ko) | 전해 살균기능을 가지는 수처리장치와 이를 통한 살균방법 | |
| CN105664525B (zh) | 从液体中吸附污染物以及吸附剂的电化学再生 | |
| CN203828015U (zh) | 果蔬清洗机 | |
| CA2196289A1 (en) | Apparatus for disinfecting fluids | |
| KR20140130758A (ko) | 잔류염소를 함유한 의료용 살균수 제조 장치 | |
| Golub et al. | Electroadsorption of bacteria on porous carbon and graphite electrodes | |
| KR100233775B1 (ko) | 정수기 살균장치 | |
| JP2000033379A (ja) | 電解殺菌装置 | |
| CN212375401U (zh) | 不分腔电解生成有效氯的空间消毒机 | |
| CN1098385A (zh) | 电滤式净水器 | |
| Geng et al. | Electrically and Electrochemically Assisted Nanofiltration: A Promising Approach for Fouling Mitigation | |
| Sekido et al. | Development of a small-sized generator of ozonated water using an electro-conductive diamond electrode | |
| JP3664274B2 (ja) | 被処理水の電解処理方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |