CN114521479A - Two-stage tobacco seedling raising method based on microbial growth promotion and air root arrangement - Google Patents
Two-stage tobacco seedling raising method based on microbial growth promotion and air root arrangement Download PDFInfo
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Abstract
The invention relates to a two-stage tobacco seedling raising method based on microbial growth promotion and air root pruning, which belongs to the technical field of seed seedling raising, and is characterized in that growth-promoting bacteria (streptomyces roseoflavus) are added into a seedling raising matrix to carry out microbial growth promotion floating seedling raising by a two-stage seedling raising technology, the time is about 4-5 weeks, and nearly 50% of seedling roots extend out of holes at the bottom of a seedling raising disc to serve as the standard for starting second-stage (air root pruning) seedling raising.
Description
Technical Field
The invention belongs to the technical field of seed seedling culture, relates to a tobacco seedling culture method, and particularly relates to a two-stage tobacco seedling culture method based on microbial growth promotion and air root arrangement.
Background
The seed seedling raising technology is one of the important links in plant production, such as plant belt seedling raising technology, shallow water tray seedling raising technology, floating seedling raising technology and the like, and can provide high-quality seedling sources for agricultural and forestry production.
The tobacco production in China mainly adopts tray seedling raising, floating seedling raising and other technologies to intensively culture tobacco seedlings, and the tobacco seedlings are transplanted in a field after the tobacco seedlings become seedlings. The floating seedling raising is one of the main seedling raising modes, the seedling raising field can be utilized more efficiently, and the seedling raising efficiency is higher. However, as the tobacco seedlings float on the nutrient solution, the heat capacity of water is large, the temperature rise is slow, the seeds are easy to germinate slowly, and the tobacco seedlings after germination grow irregularly; the external temperature in the later stage is higher, the growth speed of tobacco seedlings in the nutrient solution is higher, the quality and the stress resistance of the tobacco seedlings are relatively weaker, the seedling recovery period after transplanting in a field is long, and the infection of soil-borne pathogens is easily caused. The effect of cultivating strong seedlings is often achieved by cutting leaves and strengthening seedlings.
The microbial growth promoting floating seedling raising technology is based on the microbial growth promoting principle, and the growth promoting bacteria fermentation liquid is mixed and stirred in the floating seedling raising substrate, so that the tobacco seeds can quickly root and sprout, the growth vigor of the tobacco seedlings is consistent, and the defects of low germination rate and slow growth in the traditional floating seedling raising are effectively overcome. The air root-regulating technique is to make overhead treatment on the seedling-raising disk, so that part of the root system of the tobacco plant extends out of the drain hole at the bottom of the seedling-raising disk and is exposed in the air, the air humidity can not meet the requirement of the normal growth of the plant root on the moisture, and the extending main root tip is gradually dried up and died. The top end of the main root is cut off by air, so that the growth and development of lateral roots and fibrous roots are stimulated, the whole root system of the plant becomes developed, the seedling revival period can be shortened after the transplanting in a field, and the good effect of 'air root adjustment' is shown.
The seedling culture is one of key links in tobacco production, and the culture of strong seedlings is the basis of high-quality tobacco production. The floating seedling raising is an advanced seedling raising technology, has high production efficiency, ensures the tobacco seedlings to grow neatly and consistently, can reduce disease infection sources to the maximum extent, and is suitable for specialization and scale production of the tobacco seedlings. But the defects of slow seed germination, slow growth of the germinated plantlets, irregular growth and longer seedling revival period exist in the whole seedling raising process, and the popularization of the technology is influenced to a certain extent.
Disclosure of Invention
In order to improve and optimize the seedling culture mode of tobacco, the invention aims to provide a two-stage tobacco seedling culture method based on microbial growth promotion and air root regulation, the method is characterized in that growth-promoting bacteria are added into a seedling culture substrate to perform microbial growth promotion floating seedling culture by a two-stage seedling culture technology, the time is about 4-5 weeks, nearly 50% of seedling roots extend out of holes at the bottom of a seedling culture disc to serve as the standard for starting seedling culture in the second stage (air root regulation), and the method can enable tobacco seeds to germinate rapidly, enable the growth of tobacco seedlings to be consistent, and achieve the purposes of strengthening roots, hardening seedlings and shortening the seedling recovery period.
In order to achieve the purpose, the invention adopts the following specific scheme:
a two-stage tobacco seedling raising method based on microbial growth promotion and air root pruning comprises a first stage and a second stage;
the first stage is as follows: microbial growth-promoting floating seedling culture
Firstly, selecting streptomyces roseoflavus as growth-promoting microorganisms to prepare streptomyces roseoflavus microbial inoculum fermentation liquor;
secondly, paving an anti-seepage film at the bottom of the seedling raising pool, performing anti-seepage treatment on the pool bottom, and cutting and paving the drainage plate on the anti-seepage film;
thirdly, before sowing, adding streptomyces roseoflavus fermentation liquor into the seedling raising substrate, uniformly stirring, placing in a sealed manner for 3 days, then loading into a seedling raising tray and sowing;
fourthly, placing the sowed seedling raising tray on a drainage plate, and injecting nutrient solution into the seedling raising pool to ensure that the bottom of the seedling raising tray just contacts the liquid level; keeping the environment temperature not more than 25 ℃ and the humidity 65% during seedling raising;
after 4-5 weeks, transferring to the second stage of seedling management after nearly 50% of the roots of the seedlings extend out of the holes at the bottom of the seedling tray.
And a second stage: air root-integrated seedling raising with drainage plate
Firstly, completely discharging nutrient solution in a seedling pool with 50 percent of seedling roots extending out of the bottom of a seedling tray, paving a drainage plate on an impermeable membrane, and enabling the seedling tray to fall on the drainage plate;
secondly, spraying 1 per mill of tobacco seedling raising fertilizer nutrient solution every day;
and thirdly, strengthening management during seedling raising, and transplanting the tobacco seedlings in the field when the tobacco seedlings reach the standard of seedling formation.
As a further optimization of the tobacco seedling raising method, in the first stage, the preparation method of the streptomyces roseoflavus fermentation liquor comprises the following steps: transferring the streptomyces roseoflavus strain to a Gao's 1 culture medium for activation culture; and (3) punching a plurality of fungus cakes on the activated culture medium by using a puncher with the diameter of 0.6 cm (the inoculation amount is 5-7 fungus cakes inoculated to every 150 mL of soybean yeast liquid culture medium), inoculating the fungus cakes onto the soybean yeast liquid culture medium by using an inoculating loop, and placing the inoculated fungus cakes into a constant-temperature shaking incubator for shaking culture under the culture conditions of 28 ℃ and 170 r/min for 8-9 d to prepare the streptomyces roseoflavus fermentation broth. Furthermore, the addition amount of the streptomyces roseoflavus microbial inoculum fermentation liquor is 5-6 per mill, namely 1000kg of matrix, and 5-6L of bacterial liquid (about 5-6 kg) is added.
As a further optimization of the tobacco seedling method, in the first stage, after the tobacco is sowed in the seedling tray, the environmental temperature is kept not more than 25 ℃ and the humidity is kept at 65% during the seedling period. In addition, in the whole process of seedling culture, tobacco does not need to be subjected to leaf cutting treatment.
The nutrient solution is a special fertilizer for tobacco seedling culture, and the fertilizer is fully dissolved in a seedling culture pond according to the application method of 1 per mill.
Has the advantages that: according to the invention, through a two-stage seedling raising mode, the seeds germinate rapidly in the whole seedling raising process, the seedlings grow rapidly and grow neatly after germination, and the tobacco plant root system is developed. The tobacco seedlings can grow early and quickly after being transplanted to the field, and the seedling revival period is shortened.
Drawings
FIG. 1 is a schematic view of floating seedling; in the figure, a: polyvinyl chloride foam seedling raising trays; b: a drain plate; c: a nutrient solution; d: an impermeable membrane; e: a seedling raising pond;
FIG. 2 is a schematic view of an air monolith; in the figure, a: polyvinyl chloride foam seedling raising trays; b: a drain plate; c: an impermeable membrane; d: a seedling raising pond.
Detailed Description
The invention relates to a two-stage seedling raising technology, which relates to the principles of microbial growth promotion and air root regulation.
The specific involved principle is as follows:
the first stage is as follows: growth promoting principle of microorganism
In the stage, the principle of microbial growth promotion is adopted, the plant is propagated after being applied to soil, active substances such as cytokinin, indoleacetic acid, gibberellin, amino acid and the like are continuously secreted to improve the soil environment, and the plant indirectly acts on the plant to promote the growth of the overground part and the underground part of the plant.
Streptomyces belonging to the kingdom Prokaryotes, order Actinomycetales: (Actinobacterales) Streptomyces (Streptomycetaceae) ((Streptomycetaceae) Gram-positive bacteria have a very complex life cycle. Streptomyces functions by colonizing roots, stems, leaves, etc. of various plants as mycelia. Streptomyces is also an important biocontrol bacterium, and secondary metabolites of the Streptomyces not only can produce various antibiotics, but also can produce phytohormones. The streptomycete can inhibit various pathogenic bacteria on the tobacco and also has a certain promotion effect on the growth of the tobacco. This study was conducted by administering Streptomyces roseoflavus: (S. roseoflavus)S. roseoflavus) The generated secondary metabolite can promote the germination of tobacco seeds and the growth of seedlings, improve the germination rate of the seeds, enhance the growth capacity of the seedlings, provide a foundation for cultivating high-quality and high-yield tobacco, and has great significance for improving the yield and quality of tobacco leaves.
And a second stage: air root-trimming principle
The air root-trimming principle is adopted in the stage, part of the main root system of the plant is exposed in the air, the air humidity cannot meet the requirement of normal growth of the plant root on moisture, and the extending main root tip is gradually withered and dead. The top of the main root is cut off by air, so that the generation and development of the lateral root are promoted, and the whole root system of the plant becomes developed.
The problem that the root system is not developed due to the fact that the main root grows fast to inhibit the differentiation and growth of the lateral root is solved in both conventional seedling culture and soilless seedling culture. Attempts have been made to stimulate the growth of lateral roots by cutting the top of the main root to promote the development of the root system, but it is difficult to cut off the main roots of a large number of tobacco seedlings on a seedbed by inserting the main roots of conventional seedling raising into the soil. Although the main root extending out of the bottom hole can be cut off by lifting the seedling tray in the floating seedling raising process, the wound caused by the floating seedling raising process is easy to infect diseases, and root rot and seedling death are caused. How to effectively cut off the top end of the main root and avoid infecting germs is a problem which is always sought by people. According to the research, the seedling raising tray is placed on the drainage plate to carry out air root adjustment, so that main roots extending from the bottom hole are not in contact with soil, gradually dry and die in the air, the main roots are cut off from growing, the side roots are stimulated to grow, the whole root system of the tobacco plant becomes developed, the tobacco plant can grow early and quickly after being transplanted, and the purpose of shortening the seedling reviving period is achieved.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention.
Example 1
The invention provides a two-stage tobacco seedling raising technology based on microbial growth promotion and air root management, which comprises the following main test materials: the experimental variety is as follows: 100 parts of medium smoke; growth-promoting bacteria: streptomyces roseoflavus (A), (B)Streptomyces roseoflavus) Is provided by molecular identification of plant diseases and green prevention and control laboratories of Henan university of science and technology. Polyvinyl chloride foam seedling-raising tray, specification: 660 mm × 340 mm × 50 mm, number of cells: 20 × 10, pore diameter: 25mm × 25mm × 45 mm; and (3) impermeable membrane: a black impermeable film; a drainage plate: the protrusions with the diameter of 20mm are all commonly sold.
The seedling substrate used in the embodiment is a substrate special for floating seedling of tobacco, and is produced by Schchanke Hongkong industry and trade company Limited; the special fertilizer for tobacco seedling culture is produced by Henan Longtai fertilizer industry Co., Ltd, and is applied according to a 1 per mill application method, namely 1g of fertilizer is added into 1L of water, and the special fertilizer comprises the following main components: n + P2O5+K2O≥45%、18-9-18+TE。
Other used reagents and materials are all sold in the ordinary market if no special description is given; the adopted operation techniques and means are conventional techniques unless otherwise specified.
The specific implementation steps are as follows:
the first stage is as follows: microbial growth-promoting floating seedling culture
In the present study, Streptomyces roseoflavus (A) is usedS. roseoflavus) And (3) fermenting the liquid. The metabolite has certain promotion effect on the germination of tobacco seeds and the growth of tobacco seedlings, and can help to improve the quality of the tobacco seedlings in tobacco production.
The schematic diagram of floating seedling is shown in figure 1.
The method comprises the following specific implementation steps:
preparing fermentation liquor of streptomyces roseoflavus (S), and selecting trichoderma harzianum (T) and bacillus megatherium (B) as a reference.
Wherein, the preparation of the fermentation liquor: transferring S to a Gaoshi No. 1 culture medium, punching a plurality of fungus cakes on the activated culture medium by using a puncher with the diameter of 0.6 cm, inoculating the fungus cakes onto a soybean yeast liquid culture medium by using an inoculating loop (the inoculation amount is 5-7 fungus cakes are inoculated per 150 mL of the soybean yeast liquid culture medium), and placing the soybean yeast liquid culture medium into a constant-temperature shaking incubator for shaking culture (the culture conditions are 28 ℃, the rotating speed is 170 rmp, and shaking is 8-9 d) to prepare a Streptomyces roseoflavus fermentation liquid; b, transferring the cells to a beef extract peptone agar medium (NA) for activation culture. Using a puncher with the diameter of 0.6 cm to punch a plurality of bacterial cakes on the activated culture medium (the inoculation amount is 5-7 bacterial cakes inoculated to each 150 mLLB liquid culture medium), then using an inoculating loop to inoculate the bacterial cakes on an LB liquid culture medium, and placing the bacterial cakes into a constant-temperature shaking incubator for shaking culture (the culture condition is 30 ℃, and the culture condition is 200 r/min)-1Vibrating for 3-4 d) to prepare a bacillus megaterium fermentation liquid; t is transferred to potato dextrose agar medium (PDA) for activation culture. Using a puncher with the diameter of 0.6 cm to punch a plurality of fungus cakes (the inoculation amount is that 3-4 fungus cakes are inoculated in every 150 mLPD liquid culture medium) on the activated culture medium, then using an inoculating loop to inoculate the fungus cakes on a PD liquid culture medium, and placing the inoculated culture medium into a constant-temperature shaking incubator for shaking culture (the culture conditions are 25 ℃ and 180 r/min-1And shaking for 5-7 d) to prepare the trichoderma harzianum fermentation liquor.
Secondly, paving an anti-seepage film at the bottom of the seedling raising pool, performing anti-seepage treatment on the pool bottom, and then cutting and paving the drainage plate on the anti-seepage film.
Respectively adding S, B, T fermentation liquor (the bacterial mixing amount is 5-6 per mill of the weight of the matrix, namely 1000kg of the matrix, adding 5-6L of bacterial liquid) into a seedling matrix tobacco floating seedling special matrix (produced by macro industrial and trade company Limited in Schchangchang) before sowing, uniformly mixing, sealing and placing for 3 days, and then putting into a seedling tray and sowing.
And fourthly, placing the sowed seedling raising tray on a drainage plate, injecting a proper amount of water into the pool, and then adding a special fertilizer for tobacco seedling raising (produced by Henan Longtai fertilizer industry Co., Ltd.) according to an application method of 1 per mill and fully dissolving the special fertilizer. The bottom of the seedling raising plate just contacts the water surface. The environmental temperature is not more than 25 ℃ and the humidity is about 65% during the seedling raising period.
After 4-5 weeks, transferring to the second stage of seedling management after nearly 50% of the roots of the seedlings extend out of the bottom of the seedling tray.
And a second stage: air root-integrated seedling raising with drainage plate
This research is through directly placing the seedling tray on the bellied drain bar of 2 cm, explores the whole root effect of drain bar air.
The air whole root seedling raising schematic diagram is shown in figure 2.
The method comprises the following specific implementation steps:
firstly, completely discharging nutrient solution of about 50 percent of seedling roots from a seedling pool extending out of the bottom of a seedling tray, enabling a drainage plate to be flatly paved on an impermeable membrane, and enabling the seedling tray to fall on the drainage plate.
② spraying 1 per mill of tobacco seedling fertilizer nutrient solution every day (the nutrient solution used in the method is the same as that used in the first stage).
And enhancing management during seedling raising. Because the spray irrigation is used for providing moisture and nutrients required by the growth of tobacco seedlings, the air humidity is high, and more moisture is attached to the leaves, so that the ventilation and the moisture discharge are enhanced, and the disease outbreak is prevented.
Fourthly, transplanting the tobacco seedlings in the field when the tobacco seedlings reach the standard of seedling formation.
Test example: the growth promoting effect in the first stage and the seedling raising effect in the second stage were evaluated in example 1.
1. Microbial growth-promoting floating seedlings (first stage).
The growth promoting effect of the streptomyces roseoflavus fermentation liquor is as follows:
the tobacco growth promotion test method comprises the following steps: the test is divided into four groups, wherein the test group uses S, B, T fermentation liquor with 5-6 per mill of the weight of the seedling substrate to mix bacteria, the control group (CK) seedling substrate does not mix bacteria, the test group is placed in a hole tray, 1-2 tobacco seeds are dibbled in each hole, clear water is sprayed until the seed coating is fully cracked, a small amount of covering material is used for covering the seeds, and the cultivation is carried out in a greenhouse at the temperature of 18-25 ℃ and the relative humidity of 65%. And (4) recording the germination number after sowing for 7 d and 14 d respectively, and measuring the germination potential and the germination rate of the tobacco seeds by taking the extension of radicles and the equal length of the seeds as germination standards. Germination potential (%) = (number of germinated seeds/number of examined seeds in 7 d) × 100, germination percentage (%) = (number of all germinated seeds/number of examined seeds in 14 d) × 100. Seedling root out-of-hole ratio (%) = (number of holes of seedling roots extending out of seedling tray in 35 d/total number of holes of seedling tray) × 100. As can be seen from Table 1, the germination amount of tobacco in the nursery site to which the Streptomyces roseoflavus fermentation broth (S) was added was significantly higher than or equal to that in CK (blank control) and other fermentation broths (T: Trichoderma harzianum fermentation broth; B.megaterium fermentation broth); the root extension rate of the seedling tray added with the streptomyces roseoflavus fermentation liquor is obviously higher than that of the seedling tray added with CK and other microbial inoculum treatment groups.
TABLE 1 germination vigor, germination rate and seedling root-out-of-hole rate of tobacco seeds under growth promotion of Streptomyces roseoflavus
2. And (5) arranging roots and raising seedlings in the air by using a drainage plate (the second stage).
The effect of air root-arranging seedling culture:
the tobacco seedlings placed on a drainage plate with the height of 2 cm for air whole-root seedling raising are marked as A without leaf cutting1And the leaf cutting treatment is recorded as B1(ii) a Taking tobacco seedlings in the floating seedling raising plate as blank control, and marking the tobacco seedlings as CK without leaf cutting treatment1And leaf cutting is denoted as CK2。
As can be seen from Table 2, the root system of the tobacco seedling with the whole air root and without leaf cutting is obviously developed compared with the control, the surface area of the root system is increased by 208.98%, the volume of the root is increased by 460.81%, the number of the root tips is increased by 75.57%, the number of branches is increased by 122.33%, and other agronomic characters are also obviously superior to the control.
TABLE 2 Effect of whole air roots on the growth of the underground part of tobacco seedlings
Note: the difference between the different lower case letters in each treatment in each column indicates that the difference between treatments is statistically significant (P.ltoreq.0.05), and the upper case letters (P.ltoreq.0.01).
The time standard of entering the second stage is 4-5 weeks, and the second stage is matched to carry out air treatment on the drainage plate. Lay the drain bar in first stage be for the in-process that the second stage was grown seedlings in order to make things convenient for directly to discharge the nutrient solution in the pond of growing seedlings, make the dish of growing seedlings drop on the arch of drain bar, play an overhead effect, can save the loaded down with trivial details in the whole root mode of traditional air (need all displace the dish of growing seedlings in the pond of growing seedlings to the support of laying in addition).
Leaf cutting is one of the important steps in the tobacco seedling raising process, and aims to promote the generation and development of root systems and shorten the seedling revival period of field transplantation. The key function of the air root-adjusting technology of the drainage plate is to promote the root development of tobacco seedlings and inhibit the growth of leaves, thereby avoiding the complexity caused by leaf cutting.
The data in table 2 can demonstrate the advantage of the drainage plate over the air (without the need for leaf-cutting).
The invention belongs to a two-stage seedling raising method, combines the microorganism growth promoting and air root regulating principles, and relates to a tobacco microorganism growth promoting floating seedling raising and air root regulating seedling raising technology. Before sowing, the streptomyces roseoflavus fermentation liquor is mixed and stirred in the seedling substrate to carry out microbial growth promotion floating seedling, and the proper water and fertilizer management is ensured in the period. After 4-5 weeks, taking nearly 50% of the seedling roots extending out of the holes at the bottom of the seedling tray as the standard for starting seedling cultivation in the second stage (arranging the whole roots in the water drainage plate), strengthening the roots and hardening the seedlings. Through the two-stage seedling raising method, tobacco seeds can germinate rapidly, and the tobacco seedlings grow uniformly; strengthen the roots and harden the seedlings, improve the transplanting survival rate and shorten the seedling revival period.
It should be noted that the above-mentioned embodiments illustrate rather than limit the scope of the invention, which is defined by the appended claims. It will be apparent to those skilled in the art that certain insubstantial modifications and adaptations of the present invention can be made without departing from the spirit and scope of the invention.
Claims (5)
1. A two-stage tobacco seedling raising method based on microbial growth promotion and air root pruning is characterized in that: comprises a first stage and a second stage;
the first stage is as follows: microbial growth-promoting floating seedling culture
Selecting streptomyces roseoflavus as a growth-promoting microorganism to prepare streptomyces roseoflavus fermentation liquor;
secondly, paving an anti-seepage film at the bottom of the seedling raising pool, performing anti-seepage treatment on the pool bottom, and cutting and paving the drainage plate on the anti-seepage film;
thirdly, before sowing, adding streptomyces roseoflavus fermentation liquor into the seedling raising substrate, uniformly stirring, placing in a sealed manner for 3 days, then loading into a seedling raising tray and sowing;
fourthly, placing the sowed seedling raising tray on a drainage plate, and injecting a tobacco seedling raising fertilizer nutrient solution into the seedling raising pond to ensure that the bottom of the seedling raising tray just contacts the liquid level;
after 4-5 weeks, after half of the seedlings extend out of holes at the bottom of the seedling tray, transferring to seedling management of the second stage;
and a second stage: air root-integrated seedling raising with drainage plate
Discharging all the tobacco seedling raising fertilizer nutrient solution in the seedling raising pool with 50% of seedling roots extending out of holes at the bottom of the seedling raising disc, paving a drainage plate on an impermeable membrane, and enabling the seedling raising disc to fall on the drainage plate;
secondly, spraying 1 per mill of tobacco seedling raising fertilizer nutrient solution every day;
and thirdly, strengthening management during seedling raising, and transplanting the tobacco seedlings in the field when the tobacco seedlings reach the standard of seedling formation.
2. A tobacco seedling raising method according to claim 1, characterized in that: in the first stage, the preparation method of the streptomyces roseoflavus microbial inoculum fermentation liquor comprises the following steps: transferring the streptomyces roseoflavus strain to a Gao's 1 culture medium for activation culture; and (3) punching a plurality of bacterial cakes on the activated culture medium by using a puncher with the diameter of 0.6 cm, inoculating 5-7 bacterial cakes on every 150 mL of soybean yeast liquid culture medium, then inoculating the bacterial cakes on the soybean yeast liquid culture medium by using an inoculating loop, and placing the soybean yeast liquid culture medium into a constant-temperature shaking incubator for shaking culture under the culture conditions of 28 ℃ and 170 r/min, and shaking for 8-9 days to prepare the streptomyces roseoflavus fermentation liquid.
3. A tobacco seedling raising method according to claim 2, characterized in that: the addition amount of the streptomyces roseoflavus fermentation liquor is 5-6 per mill, namely 1000kg of seedling culture substrate, and 5-6L of streptomyces roseoflavus fermentation liquor is added.
4. A tobacco seedling raising method according to claim 1, characterized in that: in the first stage, after the tobacco is sowed in the seedling raising tray, the environmental temperature is kept not more than 25 ℃ and the humidity is kept at 65% during the seedling raising period.
5. A tobacco seedling raising method according to claim 1, characterized in that: in the whole process of seedling culture, tobacco does not need to be subjected to leaf cutting treatment.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115956502A (en) * | 2022-09-26 | 2023-04-14 | 河南科技大学 | Application of streptomyces roseoflavus LN33 in aspect of promoting growth and development of tobacco |
| CN116267483A (en) * | 2023-02-28 | 2023-06-23 | 湖南省烟草公司衡阳市公司 | One-stage cultivation method for long-leaf-age strong-root seedlings of flue-cured tobacco in rice stubble |
| CN116918668A (en) * | 2023-07-17 | 2023-10-24 | 湖南省烟草公司邵阳市公司 | Efficient transplanting method for flue-cured tobacco |
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| CN115956502A (en) * | 2022-09-26 | 2023-04-14 | 河南科技大学 | Application of streptomyces roseoflavus LN33 in aspect of promoting growth and development of tobacco |
| CN116267483A (en) * | 2023-02-28 | 2023-06-23 | 湖南省烟草公司衡阳市公司 | One-stage cultivation method for long-leaf-age strong-root seedlings of flue-cured tobacco in rice stubble |
| CN116918668A (en) * | 2023-07-17 | 2023-10-24 | 湖南省烟草公司邵阳市公司 | Efficient transplanting method for flue-cured tobacco |
| CN116918668B (en) * | 2023-07-17 | 2024-03-29 | 湖南省烟草公司邵阳市公司 | Efficient transplanting method for flue-cured tobacco |
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