CN101695284A

CN101695284A - Method for raising seedling rate of rhododendron hybrid seeds

Info

Publication number: CN101695284A
Application number: CN200910233596A
Authority: CN
Inventors: 苏家乐; 刘晓青; 李畅
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2009-10-29
Filing date: 2009-10-29
Publication date: 2010-04-21

Abstract

The invention provides a breeding method sowing rhododendron hybrid seeds on culture media, which belongs to the technical field of tissue culture. The method comprises: wrapping rhododendron seeds in filter paper for sterilization, spot-sowing the sterilized rhododendron seeds on a shoot-inducing medium accompanied with 0.2 to 0.5 mg/L of TDZ and 1.5 to 2.0 mg/L of NAA and transplanting robust-root seedlings in a matrix in which the ratio of peat to perlite is 2:1 after 30 to 45 days; and taking epicotyl from weak-root seedlings, transferring the epicotyl onto a robust-seedling medium accompanied with 0.2 to 0.5 mg/L of NAA for 25 to 30 days of culture, transferring the obtained product onto a rooting medium accompanied with 1.5 to 2.0 mg /L of NAA, 0.5 to 1.5 mg/L of IBA and 2g/L of activated carbon for 30 to 45 days of culture and transplanting the obtained product into a mixed matrix in which the ratio of the peat to the perlite is 2:1. The method has the advantage of raising the seedling rate of part of rhododendron hybrid seeds with low seedling rate by over 30 percent, thereby having positive significance for breeding novel varieties of rhododendrons.

Description

A kind of method that improves seedling rate of rhododendron hybrid seeds

One, technical field

The present invention is a kind of seedling-cultivating method that improves the low azalea seed culture medium sowing of part conventional seed planting planting percent, belongs to field of plant tissue culture technique.

Two, technical background

Azalea (Rhododendron) is as famous ornamental plants, the various widely plantations in the world.The Yunnan of China, Tibet, Sichuan one band is the place of origin and the modern distribution center of world azalea, and the most of dependence on import of azalea kind of China's commerial growing, and the azalea resource advantage of this and China's abundant extremely is not inconsistent.

Crossbreeding is the major technique means of azalea breeding of new variety, and seeding and seedling raising then is its key of success place.The azalea seed is tiny, require all very strict to sowing media and microenvironment, and the percentage of seedgermination of Different Cross Combinations and planting percent are widely different, part makes up seed germination rate and planting percent is all very high, and it is very low partly to make up the higher planting percent of seed germination rate, and combination germinating rate that also has and planting percent are all very low.

China starts late about the research of this respect, studies also less.The height of azalea percentage of seedgermination and growth of seedlings are relevant with sowing time, sowing media and temp. and humidity.The sowing time of azalea is to be advisable for 3～May; The percentage of seedgermination of sowing is high by 20%～30% than 6～August; Sowing media is good with leaf mould and leaf mould ten mountain soil (1: 1); The suitable temperature of sowing is 9 ℃～28 ℃, and it is 60%～75% that air shows degree; Substrate temperature is 6.7 ℃～25 ℃, and water content of substrate is 30%～40%.Yellow hair cuckoo seed germination rate under 25 ℃～30 ℃ conditions of constant temperature is very low, and obviously improves this external application H at 15 ℃～20 ℃ condition germination rates ₂SO ₄Processing can improve percentage of seedgermination and the regularity that sprouts with the ABT root-inducing powder.About how improving the research of azalea seed planting percent then do not appear in the newspapers.

Three, summary of the invention

Technical problem the present invention is primarily aimed at the low present situation of part azalea hybrid conventional seed planting planting percent, with

Be experiment material, the method that adopts medium aseptic seeding and tissue culture to combine improves the sowing planting percent of crossbreed, and the breeding of new variety work of azalea is had actively and profound significance.

A kind of method that improves the sub-planting percent of azalea hybrid comprises:

1) material

2) medium

Minimal medium is McCown﹠amp; Lloyd (1982), wherein sucrose changes 30g/L into, and agar powder changes 5.0g/L～5.4g/L into;

Sowing medium: additional TDZ0.2mg/L～0.5mg/L, NAA 1.5mg/L～2.0mg/L;

Strong seedling culture base: additional NAA 0.2m/L～0.5mg/L;

Root media: additional NAA 1.5mg/L～2.0mg/L, IBA 0.5mg/L～1.5mg/L, active carbon 2g/L;

Above-mentioned various medium is adjusted the pH value with sodium hydroxide and hydrochloric acid before autoclaving be 5.4;

3) method

The first step: explant sterilization: the seed with azalea is an explant, and with the filter paper parcel, with 70% alcohol-pickled 3min～5min, 0.1% mercuric chloride soaks and shakes 10min～15min after the rinsed with sterile water, rinsed with sterile water 3 times～5 times, each 1min～2min;

Second step: aseptic seeding: the seed points of the bacterium of will going out is sowed on the sowing medium, seeding row spacing 1.0cm～1.5cm, and 30d～45d will strengthen offspring and transplant in mixed-matrix;

The 3rd step: strong seedling culture: weak offspring is got its epicotyl, transfer and on the strong seedling culture base, cultivate 25d～30d;

The 4th step: culture of rootage: on root media, cultivate 30d～45d with transferring the strong sprout of high 2cm～3cm;

The 5th step: transplant: will take root transplantation of seedlings in mixed-matrix;

Condition of culture: 23 ℃ ± 2 ℃ of culturing room's temperature, intensity of illumination 1500lux～2500lux, light application time is 12h～14h/d; Mixed-matrix: peat: perlite=2: 1; Transplanting condition: 15 ℃～25 ℃ of temperature, humidity 75%～85% shades 30%～50%, and the time is kept 10d～15d.

Beneficial effect the present invention compared with prior art has following advantage and good effect:

1, the present invention adopts the mode of aseptic seeding to carry out growing seedlings of azalea hybrid, has solved in the conventional substrate seedling raising process seed and has polluted and go rotten and the low problem of external factor degree of controllability, is to improve one of the effective ways of percentage of seedgermination and planting percent and approach.

2, weak offspring being carried out strong sprout and regenerated root cultivates, it is the key technology that improves the weak tendency shoot survival percent, can make the seed development that to germinate under the conventional seed planting condition but can't Cheng Miao become whole plant, improve the planting percent of seed greatly, and obtain the variation individual plant to greatest extent.

With

The seed of hybrid combination or fruit are material, the planting percent of seed can be improved more than 30%, and the seed selection of yellow azalea is had positive effect.

Four, embodiment

The present invention is described in further detail below in conjunction with example:

Example 1

Get The seed of the maturation of hybrid combination is a material.The first step: aseptic seeding, crossbreed is not scattered to seed so that filter paper parcel is tight, with 70% alcohol-pickled 3min～5min, 0.1% mercuric chloride soaks and shakes 10min～15min after the rinsed with sterile water, rinsed with sterile water 3 times～5 times, 1min～2min at every turn; Second step: the seed of the bacterium of will going out is sowed on the sowing medium with the density points of seeding row spacing 1.0cm～1.5cm, the sowing medium is WPM+TDZ0.2mg/L～0.5mg/L+NAA 1.5mg/L～2.0mg/L, add agar 5.0g/L～5.4g/L again, sucrose 30g/L, the pH value is 5.4, autoclaving 15min～20min, place 23 ± 2 ℃ of temperature, intensity of illumination 1500lux～2500lux, light application time is in the culturing room of 12h～14h/d, 30d～45d will strengthen offspring and transplant in peat: in the mixed-matrix of perlite=2: 1, keep 15 ℃～25 ℃ of temperature, humidity 75%～85% shades 30%～50%, and the time is kept 10d～15d; Second step: strong seedling culture, weak offspring and weak tendency seedling that root system development is bad are got its epicotyl, transfer and on the strong seedling culture base, carry out strong seedling culture 25d～30d, the strong seedling culture base is WPM+NAA 0.2～05mg/L, add agar 5.0g/L～5.4g/L again, sucrose 30g/L, the pH value is 5.4, autoclaving 15min～20min, condition of culture is the same; The 3rd step: culture of rootage, on root media, cultivate 30d～45d with transferring the strong sprout of height of seedling 2cm～3cm, root media is WPM+NAA 1.5mg/L～2.0mg/L+IBA0.5mg/L～1.5mg/L, add agar 5.0g/L～5.4g/L again, sucrose 30g/L, active carbon 2g/L, the pH value is 5.4, autoclaving 15min～20min, condition of culture is the same; The 4th step: transplant, when root grows to about 0.5cm～1.0cm, when every strain has 3 of roots above, blake bottle is moved to outdoor hardening 3d～5d, and afterwash root medium is transplanted in peat: in the mixed-matrix of perlite=2: 1,15 ℃～25 ℃ of temperature, humidity 75%～85% shades 30%～50%, and the time keeps 10d～15d and gets final product.

More than be with azalea hybrid combination

Seed be that concrete experiment material has been made detailed explanation to the technical program, but this technical scheme is not limited only to this combination, with other data of the data area of technical solution of the present invention on the aseptic seeding growing seedlings of the low hybrid seed of other planting percents, reached same technique effect, do not repeated them here.

Claims

1. method that improves the sub-planting percent of azalea hybrid is characterized in that:

(1) material

Select for use hybrid combination ♀ leaflet iron oxide red *

Seed of Chinese azalea or fruit are experiment material;

(2) medium

Minimal medium is McCown ﹠amp; Lloyd (1982), hereinafter to be referred as the WPM medium, wherein sucrose changes 30g/L into, and agar powder changes 5.0～5.4g/L into;

Sowing medium: additional TDZ 0.2～0.5mg/L, NAA 15～2.0mg/L;

Strong seedling culture base: additional NAA 0.2～0.5mg/L;

Root media: additional NAA 1.5～2.0mg/L, IBA 0.5～1.5mg/L, active carbon 2g/L;

(3) method

With the extremely low hybrid combination ♀ leaflet iron oxide red of conventional substrate sowing planting percent *

The seed of Chinese azalea is an experiment material, seed or fruit are not scattered with the filter paper parcel is tight, with 70% alcohol-pickled 3～5min, 0.1% mercuric chloride soaks and shakes 10～15min after the rinsed with sterile water, rinsed with sterile water 3～5 times, each 1～2min, the back point is sowed on the sowing medium, seeding row spacing 1.0～1.5cm, 30～45d will strengthen offspring and transplant in peat: in the matrix of perlite=2: 1; Weak offspring is got its epicotyl, transfer and on the strong seedling culture base, cultivate 25～30d, transfer again and on root media, cultivate 30～45d, transplant: in the matrix of perlite=2: 1 in peat.

2. easy poinsettia cuttage method according to claim 1 is characterized in that: conditions of tissue culture is 23 ± 2 ℃ of culturing room's temperature, intensity of illumination 1500～2500lux, and light application time is 12～14h/d.