CN104054429A - Seedling culturing method of rhododendra - Google Patents
Seedling culturing method of rhododendra Download PDFInfo
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- CN104054429A CN104054429A CN201410300200.9A CN201410300200A CN104054429A CN 104054429 A CN104054429 A CN 104054429A CN 201410300200 A CN201410300200 A CN 201410300200A CN 104054429 A CN104054429 A CN 104054429A
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Abstract
The invention discloses a seedling culturing method of rhododendra. The seedling culturing method comprises the following steps: soaking seeds of the rhododendra, disinfecting, and inoculating a culture medium with the seeds for being cultured after disinfection is completed, wherein the culture medium is a 1/4 MS (Murashige and Skoog) culture medium containing 1.0-2.0 mg/L ZT (zeatin) and 0.03-0.06 mg/L of NAA (Naphthalene Acetic Acid). According to the seedling culturing method, a hormone with a special concentration and a special variety is added to the culture medium so that germination of the rhododendra seeds can be promoted to form clustering seedlings, thus the breeding coefficient is increased and an important significance is achieved for preservation of high-quality germplasms. In addition, the hormone with a special concentration and a special variety is added to the culture medium so that the seeds can be germinated within 10 days, with a seed germination rate of reaching up to about 48 percent.
Description
Technical field
The invention belongs to plant biotechnology field, relate in particular to the seedling-cultivating method of a kind of spring cuckoo.
Background technology
Azalea is Ericaceae (Ericaceae) Rhododendron (Rhododendron) plant, is one of China's ten large famous flowers.China azalea is of a great variety, aboundresources, and interspecific difference is larger.The whole world approximately has azalea more than 1000 to plant, and China accounts for 50% left and right.Azalea is Woody flower, and the difference regional according to growth, is evergreen, half evergreen and deciduous plant.Generally, according to form, florescence, parent and source, azalea can be divided into a mao cuckoo, eastern cuckoo, Xia Juan and the large class of western cuckoo four (Huang Maoru and strong letter good 1984), traditionally, hair cuckoo and eastern cuckoo be collectively referred to as to spring cuckoo.
Spring, cuckoo had very high ornamental value, large flower and brilliant color, and germination is strong, resistance to pruning.Plantation in flakes, its scenery is particularly grand.In gardens, be widely used, often do greening and use.Plant in the other extremely scenery of U.S. that all can form of grove, sylvan life, small stream limit, lawn and building.Can do potted plant and moulding cuckoo simultaneously.
The modes of reproduction of azalea mainly contains cuttage, propagation by grafiting.Yet seedling plant strain growth that seed obtains is healthy and strong, well developed root system, vitality are vigorous, strong to adaptive capacity to environment, can keep parent's merit, especially, in the crossbreeding research of cuckoo, conventionally adopt seed to breed.
In existing seminal propagation research, for guaranteeing the breeding of cuckoo, the germination rate of common concern cuckoo seed, the survival rate of plant etc., as, publication number is that the Chinese patent literature of CN101999291B discloses a kind of method for sowing alpine rhododendron by utilizing sphagnum, the characteristic of utilizing bog moss and marchantia mutually to restrict in vegetative period, can be in alpine rose planting seed and process of growth, effectively control marchantia growth, for alpine rose seed growth provides good ecotope, prevent that cuckoo seedling is because of the covering death of noble liverwort.Publication number is that the Chinese patent literature of CN 1166271 discloses a kind of method for reproducing seeds of Rhododendron delavayi Franch, and the breeding that is delavay rhododendron seed by improved culture medium matter provides good environmental condition, makes the reproduction rate of delavay rhododendron seed up to more than 70%.Publication number is the precision seedling-cultivating method that the Chinese patent literature of CN 103299819A discloses a kind of alpine rose, three steps of environment control of being sprouted after cultivation, the meticulous field planting of seed, field planting by the radicle of seed form, by controlling the condition of culture in each step, the seedling fostering time is short is only 60～65d, seedling early growth is neat, healthy and strong, and planting percent is up to 85～90%.But in the reproductive process of seed, each seed only can be sprouted and grow up to a plant, and reproduction coefficient is low, if take measures, by a seed, obtain a plurality of plant, by greatly improving the reproductive efficiency of cuckoo, reduce costs so, also significant for the preservation of elite germplasm.
Summary of the invention
The invention provides the seedling-cultivating method of a kind of spring cuckoo, can induce seed germination to form the seedling of growing thickly.
Spring cuckoo a seedling-cultivating method, comprising:
Get the seed of spring cuckoo, after seed soaking, sterilization, is inoculated in seed on medium and cultivates after having sterilized; Described medium is the 1/4MS medium that contains 1.0～2.0mg/L ZT and 0.03～0.06mg/LNAA.
The application is placed in medium by spring cuckoo seed and cultivates, and by add ZT (zeatin) and NAA (methyl α-naphthyl acetate) in medium, both actings in conjunction under suitable concentration, can make spring cuckoo seed germination form the seedling of growing thickly.
Preferably, in described medium, the concentration of described NAA is 0.05mg/L, and the concentration of described ZT is 1.0mg/L or 2.0mg/L.Under this concentration, all spring cuckoo seeds that can sprout all can form the seedling of growing thickly.The concentration of ZT is preferably 1.0mg/L, and under this concentration, the germination rate of seed is higher.
Described spring kind of cuckoo is ' The purple butterfly ', is to guarantee germination rate, and seed is preferably taken from ripe uncracked green capsule.
Vernalization need first be soaked seed before cultivating, and seed soaking can impel spring cuckoo seed to germinate ahead of time, during seed soaking, seed is placed in to the water 20～30 hours of 18～23 ℃, and the temperature of water is 20 ℃ preferably, and the time of seed soaking is 24 hours preferably.
Suitable temperature and illumination condition are conducive to spring cuckoo seed germination and growth of seedling, and general, the temperature of described cultivation is 22～25 ℃, is preferably 24 ℃.The illumination condition of described cultivation is: intensity of illumination is 2000～2500lx, and light application time is 12～14h/d, and preferred, intensity of illumination is that 2500lx, light application time are 12h/d.
For guaranteeing the supply of plant nutrition, on medium, cultivate a period of time (about 60 days), a replaceable subculture, the formula of medium is constant.
Compared with prior art, beneficial effect of the present invention is:
The present invention adds the hormone of certain concentration and particular types in medium, can impel spring cuckoo seed germination to form the seedling of growing thickly, and has improved reproduction coefficient, significant to the preservation of elite germplasm.
Method of the present invention is easy and simple to handle, seed is directly placed in to medium and cultivates, and culture medium prescription is simple.
The present invention adds the hormone of certain concentration and particular types in medium, and seed can be sprouted about 10 days, and seed germination rate can be up to 48% left and right.
Accompanying drawing explanation
Fig. 1 is control group growth of seedling situation, and wherein, A is not for adding hormone, and B is 0.05mg/LNAA.
Fig. 2 is the seedling induction situation of growing thickly under NAA and ZT synergy, and wherein, A is 0.05mg/LNAA, 1.0mg/L ZT, and B is 0.05mg/L NAA, 2.0mg/L ZT.
Fig. 3 is that spring cuckoo seed is induced the situation schematic diagram of the seedling of growing thickly at 0.05mg/L NAA+1.0mg/L ZT+1/4MS medium.
Fig. 4 is growth of seedling situation under ZT independent role, and A is 1.0mg/L ZT, and B is 2.0mg/LZT.
Embodiment
Below in conjunction with specific embodiment, the present invention is further explained.
Embodiment 1
(1) seed preparation
Mid or late November, ripe at spring cuckoo ' The purple butterfly ' capsule, but during for uncracked green capsule still, adopted down, after use liquid detergent water soaking 20-30min, be placed under flowing water and rinse 1-2h.With banister brush, scrub gently after the foul on capsule surface, with filter paper, blot the moisture on capsule surface, with culture dish, install (without covering culture dish lid).Be put in the indoor container that silica-gel desiccant is housed, be dried, until capsule natural cracking.
(2) seed soaking
Before seed soaking, by the capsule strip off of cracking, without spring cuckoo seed is carried out disinfection.After impurity elimination, select the seed of mature and plump, place it in the warm water of 20 ℃ and soak 24h.
(3) medium preparation and sterilization
The medium that preparation contains hormon, the horizontal high voltage sterilizing of going forward side by side (some hormone need to, after autoclaving, join in medium).
CK group:
①1/4MS②1/4MS+0.05mg/L NAA；
ZT group:
①1/4MS+0.05mg/L NAA+0.5mg/L KT②1/4MS+0.05mg/L NAA+1.0mg/L KT③1/4MS+0.05mg/L NAA+2.0mg/L KT④1/4MS+0.5mg/L KT⑤1/4MS+1.0mg/L KT⑥1/4MS+2.0mg/L KT；
(4) seed disinfection
The seed that taking-up has been soaked, blots the moisture of the surface of the seed with filter paper.First use 70% alcohol-pickled 30s, with after aseptic water washing 3-5 time, seed is soaked in to 15-18min in 0.1% mercuric chloride water, then uses aseptic water washing 3-5 time, washing time is no less than 3min at every turn.After sterilization, with filter paper, blot the moisture of the surface of the seed.
(5) aseptic seeding
The seed of sterilizing is inoculated in respectively in the medium that contains variable concentrations, hormon, each processes 30, repeats for 3 times.After inoculation, in the Sterile culture room that to be positioned over temperature and to be 24 ℃, intensity of illumination and be 2500lx, light application time be 12h/d, cultivate.Cultivate after 60d, change fresh culture, culture medium prescription is filled a prescription during with seed germination identical.Observe the situation that forms the seedling of growing thickly.
Cuckoo Seed Development of lower spring of the different disposal situation of seedling of growing thickly, in Table 1, table 2.
Table 1CK group spring cuckoo seed germination and the seedling of growing thickly induction situation
| Hormone (mg/L) | Germination rate (%) | Sprout time (d) | Induce the seedling rate (%) of growing thickly |
| NAA0.05 | 58.9 | 11 | 0 |
| 1/4MS blank | 55 | 13 | 0 |
The situation of table 2 hormone ZT to spring cuckoo seed germination and the seedling of growing thickly induction
In table, data can be found out, the independent role of NAA and ZT all can not make the spring cuckoo Seed Development seedling of growing thickly.Only have NAA and ZT to take suitable concn (ZT is 1mg/L or 2mg/L, and NAA is 0.05mg/L) acting in conjunction and the spring cuckoo kind period of the day from 11 p.m. to 1 a.m, spring cuckoo seed could form the seedling of growing thickly, and induction is grown thickly the ratio of seedling up to 100%.Each seed can be induced the seedling 10-20 strain of growing thickly.The method can effectively induce spring cuckoo seed directly to form the seedling of growing thickly, and its method is easy, and formula is simple, and cost is low.Meanwhile, significant for the preservation of elite germplasm.
Claims (7)
- Spring cuckoo a seedling-cultivating method, it is characterized in that, comprising:Get the seed of spring cuckoo, after seed soaking, sterilization, is inoculated in seed on medium and cultivates after having sterilized; Described medium is the 1/4MS medium that contains 1.0～2.0mg/L ZT and 0.03～0.06mg/LNAA.
- 2. seedling-cultivating method as claimed in claim 1, is characterized in that, in described medium, the concentration of described NAA is 0.05mg/L.
- 3. seedling-cultivating method as claimed in claim 1, is characterized in that, in described medium, the concentration of described ZT is 1.0mg/L or 2.0mg/L.
- 4. seedling-cultivating method as claimed in claim 1, is characterized in that, described seed is taken from the ripe uncracked green capsule of spring cuckoo.
- 5. seedling-cultivating method as claimed in claim 1, is characterized in that, the method for described seed soaking comprises: seed is placed in to the water 20～30 hours of 18～23 ℃.
- 6. seedling-cultivating method as claimed in claim 1, is characterized in that, the temperature of described cultivation is 22～25 ℃.
- 7. seedling-cultivating method as claimed in claim 1, is characterized in that, the illumination condition of described cultivation is: intensity of illumination is 2000～2500lx, and light application time is 12～14h/d.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116369311A (en) * | 2023-04-04 | 2023-07-04 | 中国电建集团贵阳勘测设计研究院有限公司 | Preservation, germination and seedling raising method for improving germination rate of rhododendron alpine seeds |
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- 2014-06-26 CN CN201410300200.9A patent/CN104054429B/en not_active Expired - Fee Related
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| CN101095399A (en) * | 2006-06-29 | 2008-01-02 | 戴悦 | Protogenic alpine azalea introduction and acclimatization method |
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| CN116369311A (en) * | 2023-04-04 | 2023-07-04 | 中国电建集团贵阳勘测设计研究院有限公司 | Preservation, germination and seedling raising method for improving germination rate of rhododendron alpine seeds |
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