CN105309289A - Cultivation method for improving northern protected tomato resistibility to root-knot nematode - Google Patents
Cultivation method for improving northern protected tomato resistibility to root-knot nematode Download PDFInfo
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- CN105309289A CN105309289A CN201510665935.6A CN201510665935A CN105309289A CN 105309289 A CN105309289 A CN 105309289A CN 201510665935 A CN201510665935 A CN 201510665935A CN 105309289 A CN105309289 A CN 105309289A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a cultivation method for improving northern protected tomato resistibility to root-knot nematode, the method comprising: A. Preparation of seedling substrate: the seedling substrate comprises, by volume, 2-4 parts of rice husk, 1-3 parts of fermented chicken manure, 1-2 parts of river sand, and 1 part of perlite. The seedling substrate is prepared by well mixing and disinfecting those raw materials. B. Mycorrhizal fungi is inoculated on the seedling substrate to form tomato mycorrhizal inoculant, then, tomato seeds are sowed and cultured on the tomato mycorrhizal inoculant, and tomato seedlings are sprouted. C. Before tomato seedling transplanting, phosphorus solubilizing bacteria is inoculated at root sites of tomato seedlings; tomato mycorrhizal inoculant is transplanted together with the tomato seedlings when transplanting. Soil is covered. Cultivation management is performed to tomato seedlings. The cultivation method has advantages of simple technology and easy operation so that industrialization production of tomato seedlings is facilitated.
Description
Technical field:
The invention belongs to agricultural production and microbe application field, be specifically related to a kind of cultivation method improving the anti-root-knot nematode of northern facility tomato.
Background technology:
Tomato is one of main economic crops of China, is the guarantee of increasing peasant income.Facilities vegetable tomato intensive degree is higher, and tomato rhizosphere soil continuous cropping obstacle phenomenon is day by day serious, and particularly root-knot nematode is spread unchecked.Current tomato in China plantation soil-borne disease occurs serious, continuous cropping causes tomato planting soil quality to decline, and every year because of the root-knot nematode underproduction 30%, accounts for more than 40% of tomato planting area, wherein with the impact of root-knot nematode in northern district facility tomato planting process, govern tomato intensive manufacture.In Shandong facilities vegetable plantation intensive degree district higher, root-knot nematode continuous cropping obstacle phenomenon is serious, and tomato production is subject to serious restriction.In order to improve the anti-root-knot nematode of tomato in agricultural production, produce the stewing canopy mode of upper main employing at present and carry out, and tomato cultivation by setting seedlings is main planting type, tomato soil continuous cropping obstacle and tomato planting are produced exists larger contradiction.
Current research shows, and Arbuscular Mycorrhizal Fungi fungi not only can improve the vegetable plants such as tomato to element absorption such as phosphorus, can reduce simultaneously pathogenic microorganism etc. the impact of disease, caused academic interest more widely.But because mycorrhizal fungi can not direct cultured in vitro, seriously constrain the application of mycorrhizal fungi on tomato, at present mycorrhizal fungi and tomato are inoculated the preferred manner that nursery is tomato seedling, but it is very not outstanding that mycorrhizal fungi acts on performance large Tanaka, show that mycorrhizal fungi individual contributions effect in rhizospheric microorganism state environment is lower, must with other microorganism actings in conjunction.The matrixing nursery of tomato has been the important key link of tomato production, and matrixing nursery measure is that tomato seedling cultivates an important ring.
Summary of the invention:
The object of this invention is to provide that a kind of method is simple, cost is lower, not only can reduce root-knot nematode and tomato is infected, can also tomato growth be promoted, improve the cultivation method of the anti-root-knot nematode of raising northern facility tomato of tomato anti-root-knot nematode ability.
The present invention is first by the preparation of seedling medium, then mycorrhizal fungi and tomato seedling symbiosis is cultivated, again mycorhiza tomato seedling is transplanted cultivation, before tomato seedling is transplanted, rhizosphere phosphorus-solubilizing bacteria liquid bacterial agent is utilized to inoculate, symbiotic relation is set up to make Mycorrhizal tomato and phosphorus-solubilizing bacteria, at tomato nutrient reproductive phase regulation and control rhizospheric microorganism environment, can promote that Mycorrhizal tomato and phosphorus-solubilizing bacteria set up symbiotic relation, improve the colonization status of mycorrhizal fungi, greatly can improve the anti-root-knot nematode ability of tomato, reduce root-knot nematode infecting tomato, improve tomato growth, thus realize object of the present invention.
The cultivation method of the anti-root-knot nematode of raising northern facility tomato of the present invention, is characterized in that, comprise the following steps:
The preparation of A, seedling medium: described seedling medium is part meter by volume, comprises rice husk 2-4 part, fermentation poultry dung 1-3 part, river sand 1-2 part and perlite 1 part, mixes, after sterilizing (under 121 DEG C of conditions, sterilizing 40-60 minute, takes out and is cooled to 30-40 DEG C), obtained seedling medium;
B, on seedling medium Arbuscular Mycorrhizal Fungi fungi, form mycorhiza tomato Inoculant, then on this mycorhiza tomato Inoculant, sow tomato seeds, cultivate, grow tomato seedling;
C, tomato seedling transplant before, tomato seedling root system position inoculation phosphorus-solubilizing bacteria, during transplanting, transplant tomato seedling together with mycorhiza tomato Inoculant.Then to the tomato seedling earthing after transplanting, cultivation management is carried out to tomato seedling.
Described mycorrhizal fungi is preferably Glomus mosseae (Glomusmosseae).
Described phosphorus-solubilizing bacteria is preferably Pseudomonas alcaligenes (Pseudomonasalcaligenes).
Described Arbuscular Mycorrhizal Fungi fungi on seedling medium, form mycorhiza tomato Inoculant, then on this mycorhiza tomato Inoculant, tomato seeds is sowed, cultivate, grow tomato seedling and be preferably: on seedling medium, inoculate Glomus mosseae, form mycorhiza tomato Inoculant, then on this mycorhiza tomato Inoculant, tomato seeds is sowed, cultivate, grow tomato seedling, guarantee that individual plant tomato seedling is containing 90 ~ 150 Glomus mosseae spores.
Preferably, the Glomus mosseae solid fungicide containing 40 Glomus mosseae spores with every gram mixes according to the ratio that volume ratio is 1:25 ~ 35 with seedling medium, form mycorhiza tomato Inoculant, then be filled in the seedling-cultivating tray of foam hole, mycorhiza tomato Inoculant lower face 2 ~ 3cm place sowing tomato seeds in cave, cultivate, grow tomato seedling.
Preferably, described Glomus mosseae solid fungicide is prepared by the following method: to cross the river sand of 2mm sieve for matrix, after high-temperature sterilization, place 10 ~ 14 days under nature, then put into basin, every 2kg matrix inoculation 100-150g Glomus mosseae bacterium original seed, mix thoroughly, add the water of matrix weight 18 ~ 25%, sowing sorghum seeds, after five months, gather in the crops the host plant that infected by Glomus mosseae and the matrix original seed containing Glomus mosseae spore, the outer mycelia of root; Then sorghum field upper part is cut off, shred root segment, mix with matrix original seed, be i.e. obtained Glomus mosseae solid fungicide.Every gram of Glomus mosseae solid fungicide contains 40 Glomus mosseae spores.
Before tomato seedling transplanting, at tomato seedling root system position, inoculation phosphorus-solubilizing bacteria is preferably: transplant in tomato seedling and start for first 2 weeks, inoculate Pseudomonas alcaligenes liquid bacterial agent 2 ~ 4 times at tomato seedling root system position, and each every strain tomato seedling inoculum concentration is 10
3~ 10
5individual Pseudomonas alcaligenes.
Described Pseudomonas alcaligenes liquid bacterial agent is prepared by the following method: contain in the wheat bran leaching juice of 5.0 ~ 16.0g calcium carbonate at every 1000ml, access Pseudomonas alcaligenes, cultivates under normal condition, obtain Pseudomonas alcaligenes liquid bacterial agent.
The tomato seedling (tomato seedling of seedling medium and Arbuscular Mycorrhizal Fungi fungi and phosphorus-solubilizing bacteria cultivation) cultivated according to method of the present invention, through land for growing field crops and pot experiment checking, can significantly reduce root-knot nematode to infect Tomato Root System, strengthen the anti-root-knot nematode ability of tomato, alleviate cell membrane lipid extent of peroxidation, stabilize membrane structure, reduce cell membrane permeability, significantly improve and transplant rear tomato to the anti-infection ability of root-knot nematode, alleviate pathogenic microorganism injury, significant to northern facility tomato production.Compared with conventional tomato nursery, the present invention can improve tomato biological amount 10-15%, improves or significantly improves tomato yield, and adopt this method can reach 38-56% to tomato root-knot eelworm control efficiency, tomato production reaches the basic demand of high yield and high quality.
By tomato seedling Arbuscular Mycorrhizal Fungi fungi, make the Mycorrhizal of tomato seedling process, Mycorrhizal tomato seedling inoculates phosphorus-solubilizing bacteria, make Tomato Root System and mycorrhizal fungi, phosphorus-solubilizing bacteria set up symbiotic relation, not only improve rhizosphere environment, improve mycorrhizal fungi and infect, and improve the anti-root-knot nematode ability of tomato, strengthen Tomato Root System opposing other pathogenic microorganisms extraneous to infect, reach the effect controlling tomato root-knot eelworm, thus effectively improve the ability of the anti-root-knot nematode of tomato.Method of the present invention, technique is simple, and convenient operation, is thus easy to carry out the production of batch production tomato seedling.
The Pseudomonas alcaligenes (Pseudomonasalcaligenes) used in the present invention and Glomus mosseae (Glomusmosseae) are bacterial strain disclosed in prior art.Wherein Pseudomonas alcaligenes, (Pseudomonasalcaligenes) openly record in the literature, (bibliography: LinZhang & JiequnFan & XiaodongDing & XinhuaHe & FusuoZhang & GuFeng.Hyphosphereinteractionsbetweenanarbuscularmycorrh izalfungusandaphosphatesolubilizingbacteriumpromotephyta temineralizationinsoil), Glomus mosseae (Glomusmosseae) openly records (bibliography: XiaodongDing & XinhuaSui & FangWang & JunhuaGao & XinhuaHe & FusuoZhang & JunchengYang & GuFeng.SynergisticinteractionsbetweenGlomusmosseaeandBra dyrhizobiumjaponicuminenhancingprotonreleasefromnodulesa ndhyphae.Mycorrhiza in the literature, 2012, 22:51-58), above-mentioned two microorganism the applicant also hold, and ensure to rise in 20 years in the present patent application day to provide to the public.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1
1, the preparation of tomato seedling substrate: get 3 parts of rice husks according to volume ratio, (compost fermentation legal system is standby: ventilating, high land, and pile up in heaps by the chicken manure of band bedding and padding, outside mud is closed for 2 parts of fermentation poultry dungs; Fermentation time is about 15 days summer, about 50 days winter), the perlite (rice husk: fermentation poultry dung: river sand: perlite=3:2:2:1) of 2 parts of particle diameters to be the river sand of 1mm and 1 part of particle diameter be 1mm, mix, form mixture, after by mixture under 121 DEG C of conditions, sterilizing 50 minutes, take out be cooled to 40 DEG C, obtained seedling medium, for subsequent use.
2, the preparation method of Glomus mosseae solid fungicide: to cross the river sand of 2mm sieve for matrix, after high-temperature sterilization (each 121 DEG C of high pressure steam sterilization 45min), place 10 days under nature, then fill two kilograms and put into plastic basin, inoculation 100g Glomus mosseae bacterium original seed, mix thoroughly, add the running water of matrix weight 25%, sowing sorghum seeds, keep normal growth, the host plant that infected by Glomus mosseae bacterium and the matrix original seed containing Glomus mosseae bacterium spore, the outer mycelia of root after six months, can be obtained; Sorghum field upper part is cut off, shreds root segment, and mix with the matrix containing Glomus mosseae bacterium spore, the outer mycelia of root, i.e. obtained Glomus mosseae solid fungicide.Every gram of Glomus mosseae solid fungicide contains 40 Glomus mosseae spores.
3, this Glomus mosseae solid fungicide is mixed with seedling medium according to volume ratio 1:25, and obtain mycorhiza tomato Inoculant; Carry out tomato seedling with substrate seedling method, mycorhiza tomato Inoculant is filled in the hole of foam hole seedling-cultivating tray, then at mycorhiza tomato Inoculant lower face 2 ~ 3cm place sowing tomato seeds, cultivate, grow tomato seedling.
4, the preparation of Pseudomonas alcaligenes liquid bacterial agent and inoculation: medium: every 1000ml wheat bran soak in juice and contains 5g calcium carbonate (CaCO
3), pH6.5; Be inoculated into by Pseudomonas alcaligenes in the above-mentioned medium of 1000ml, 200rpm, 28 DEG C, cultivate 24 hours, obtain Pseudomonas alcaligenes liquid bacterial agent, the bacterium number of every milliliter of bacterium liquid reaches 1.0 × 10
9individual/mL.Transplant in tomato seedling and start for first 2 weeks, inoculate twice Pseudomonas alcaligenes liquid bacterial agent at tomato seedling root system position, each every strain tomato seedling inoculum concentration is 7 × 10
4individual Pseudomonas alcaligenes, during transplanting, transplants tomato seedling, earthing together with mycorhiza tomato Inoculant, then manages tomato seedling conventional cultivation.
Effect of inoculation:
According to the method described above, the Field trial demonstration test in multiple tomato place of production in Shandong Province, its result is as shown in table 1.As can be seen from Table 1, relative to contrast (conventional substrate tomato seedling), tomato chlorophyll and superoxide dismutase content can be significantly improved at tomato inoculation Glomus mosseae and Pseudomonas alcaligenes according to the present embodiment, thus alleviate cell membrane lipid extent of peroxidation, stabilize membrane structure, reduce cell membrane permeability, significantly can reduce the injury of the early stage root-knot nematode of tomato seedling after transplanting, significant to the tomato production of Shandong District.Method of the present invention can improve tomato biological amount 13 ~ 18%, and therefore slightly can improve or significantly improve tomato yield 12 ~ 18%, tomato production reaches the basic demand of high yield and high quality.The root knot nematode control effect of this method to tomato is adopted to reach 28-47%.
Table 1: matrixing nursery inoculation Glomus mosseae and Pseudomonas alcaligenes are to the effect of Development of Tomato and root knot nematode control
Embodiment 2:
1, the preparation of tomato seedling substrate: the perlite (rice husk: fermentation poultry dung: river sand: perlite=2:1:1:1) according to the river sand that volume ratio gets 2 parts of rice husks, 1 part of fermentation poultry dung, 1 part of particle diameter are 1mm and 1 part of particle diameter being 1mm, mix, form mixture, after by mixture under 121 DEG C of conditions, sterilizing 40 minutes, taking-up is cooled to 30 DEG C, obtained seedling medium, for subsequent use.
2, the preparation method of Glomus mosseae solid fungicide: to cross the river sand of 2mm sieve for matrix, after high-temperature sterilization (each 121 DEG C of high pressure steam sterilization 45min), place 14 days under nature, then fill two kilograms and put into basin, inoculation 100g Glomus mosseae bacterium original seed, mix thoroughly, add the running water of matrix weight 20%, the seed of sowing Chinese sorghum and shallot, keep normal growth, the host plant that infected by Glomus mosseae bacterium and the matrix containing Glomus mosseae bacterium spore, the outer mycelia of root after six months, can be obtained; Chinese sorghum and shallot acrial part are cut off, shreds root segment, and mix with the matrix containing Glomus mosseae bacterium spore, the outer mycelia of root, i.e. obtained Glomus mosseae solid fungicide.Every gram of Glomus mosseae solid fungicide contains 40 Glomus mosseae spores.
2, this Glomus mosseae solid fungicide is mixed with seedling medium according to volume ratio 1:35, and obtain mycorhiza tomato Inoculant.Carry out tomato seedling with substrate seedling method, mycorhiza tomato Inoculant is filled in the hole of foam hole seedling-cultivating tray, then at mycorhiza tomato Inoculant lower face 2 ~ 3cm place sowing tomato seeds, cultivate, grow tomato seedling.
3, the inoculation of Pseudomonas alcaligenes liquid bacterial agent: prepare medium: containing 6g calcium carbonate (CaCO in every 1000ml wheat bran leaching juice
3), pH7.5; Be inoculated into by Pseudomonas alcaligenes in the above-mentioned medium of 1000ml, 200rpm, 28 DEG C, cultivate 24 hours, obtain Pseudomonas alcaligenes liquid bacterial agent, the bacterium number of every milliliter of bacterium liquid reaches 1.0 × 10
9individual/mL.Transplant in tomato seedling and start for first 2 weeks, inoculate 4 Pseudomonas alcaligenes liquid bacterial agents at tomato seedling root system position, each every strain tomato seedling inoculum concentration is 1 × 10
5individual Pseudomonas alcaligenes, during transplanting, transplants tomato seedling, earthing together with mycorhiza tomato Inoculant, then manages tomato seedling conventional cultivation.
Effect of inoculation:
According to the method described above, verify through field experiment, relative to contrast (not inoculating Glomus mosseae and phosphorus-solubilizing bacteria), that implements according to the present embodiment improves tomato biological amount 19.4% at tomato inoculation Glomus mosseae and phosphorus-solubilizing bacteria, significantly improve chlorophyll in tobacco leaf and superoxide dismutase content, thus alleviate cell membrane lipid extent of peroxidation, stabilize membrane structure, reduce cell membrane permeability, the anti-root-knot nematode ability of tomato seedling after transplanting can be significantly improved.The control efficiency of this method to the root-knot nematode ability of tomato is adopted to reach 28-52%.
Embodiment 3:
1, the preparation of tomato seedling substrate: the perlite (rice husk: fermentation poultry dung: river sand: perlite=4:3:1:1) according to the river sand that volume ratio gets 4 parts of rice husks, 3 parts of fermentation poultry dungs, 1 part of particle diameter are 1mm and 1 part of particle diameter being 1mm, mix, form mixture, after by mixture under 121 DEG C of conditions, sterilizing 40 minutes, taking-up is cooled to 30 DEG C, obtained seedling medium, for subsequent use.
2, the preparation method of Glomus mosseae solid fungicide: to cross the river sand of 2mm sieve for matrix, after high-temperature sterilization (each 121 DEG C of high pressure steam sterilization 45min), place 10 days under nature, then get in two kilograms of loading basins, inoculation 150g Glomus mosseae bacterium original seed, mix thoroughly, add the running water of matrix weight 18%, sowing sorghum seeds, keep normal growth, the host plant that infected by Glomus mosseae bacterium and the matrix containing Glomus mosseae bacterium spore, the outer mycelia of root after six months, can be obtained; Sorghum field upper part is cut off, shreds root segment, and mix with the matrix containing Glomus mosseae bacterium spore, the outer mycelia of root, i.e. obtained Glomus mosseae solid fungicide.Every gram of Glomus mosseae solid fungicide contains 40 Glomus mosseae spores.
3, Glomus mosseae solid fungicide is mixed with seedling medium according to volume ratio 1:30, and obtain mycorhiza tomato Inoculant.Tomato seedling is carried out with substrate seedling method, mycorhiza tomato Inoculant is filled in the hole of foam hole seedling-cultivating tray, ensure that in seedling-cultivating tray hole, individual plant is containing 90 ~ 150 mycorrhizal fungal spores, then at mycorhiza tomato Inoculant lower face 2 ~ 3cm place sowing tomato seeds, thinning after seed sprouting, every Kong Liumiao 1.
4, Pseudomonas alcaligenes solid fungicide is inoculated: the preparation of Pseudomonas alcaligenes liquid bacterial agent: medium: containing 16g calcium carbonate (CaCO in every 1000ml wheat bran leaching juice
3), pH6.0; Be inoculated into by Pseudomonas alcaligenes in the above-mentioned medium of 1000ml, 200rpm, 28 DEG C, cultivate 24 hours, obtain Pseudomonas alcaligenes liquid bacterial agent, the bacterium number of every milliliter of bacterium liquid reaches 0.5 × 10
9individual/mL.Transplant in tomato seedling and start for first 2 weeks, within the 7th, 14 day, respectively inoculate a Pseudomonas alcaligenes liquid bacterial agent at tomato seedling root system position, each every strain tomato seedling inoculum concentration is 1 × 10
3individual Pseudomonas alcaligenes, during transplanting, transplants tomato seedling, earthing together with mycorhiza tomato Inoculant, then manages tomato seedling conventional cultivation.
Effect of inoculation:
According to the method described above, verify through field experiment, relative to contrast (conventional seedbed system matrix and do not inoculate Glomus mosseae and phosphorus-solubilizing bacteria), that implements according to the present embodiment can improve tomato biological amount 10% at tomato inoculation Glomus mosseae and phosphorus-solubilizing bacteria, significantly improve chlorophyll in tobacco leaf and superoxide dismutase content, thus alleviate cell membrane lipid extent of peroxidation, stabilize membrane structure, reduce cell membrane permeability, in anti-root-knot nematode, play obviously acting on.The root knot nematode control effect of this method to tomato is adopted to reach 31-54%.
Claims (8)
1. improve a cultivation method for the anti-root-knot nematode of northern facility tomato, it is characterized in that, comprise the following steps:
The preparation of A, seedling medium: described seedling medium is part meter by volume, comprises rice husk 2-4 part, fermentation poultry dung 1-3 part, river sand 1-2 part and perlite 1 part, mixes, after sterilizing, obtained seedling medium;
B, on seedling medium Arbuscular Mycorrhizal Fungi fungi, form mycorhiza tomato Inoculant, then on this mycorhiza tomato Inoculant, sow tomato seeds, cultivate, grow tomato seedling;
C, tomato seedling transplant before, tomato seedling root system position inoculation phosphorus-solubilizing bacteria, during transplanting, transplant tomato seedling, earthing together with mycorhiza tomato Inoculant, cultivation management carried out to tomato seedling.
2. cultivation method according to claim 1, is characterized in that, described mycorrhizal fungi is Glomus mosseae (Glomusmosseae).
3. cultivation method according to claim 1, is characterized in that, described phosphorus-solubilizing bacteria is Pseudomonas alcaligenes (Pseudomonasalcaligenes).
4. cultivation method according to claim 1, it is characterized in that, described Arbuscular Mycorrhizal Fungi fungi on seedling medium, form mycorhiza tomato Inoculant, then on this mycorhiza tomato Inoculant, tomato seeds is sowed, cultivate, growing tomato seedling concrete steps is: on seedling medium, inoculate Glomus mosseae, form mycorhiza tomato Inoculant, then on this mycorhiza tomato Inoculant, tomato seeds is sowed, cultivate, grow tomato seedling, guarantee that individual plant tomato seedling is containing 90 ~ 150 Glomus mosseae spores.
5. cultivation method according to claim 1, it is characterized in that, the Glomus mosseae solid fungicide containing 40 Glomus mosseae spores with every gram mixes according to the ratio that volume ratio is 1:25 ~ 35 with seedling medium, form mycorhiza tomato Inoculant, then be filled in the seedling-cultivating tray of foam hole, in cave, mycorhiza tomato Inoculant lower face 2 ~ 3cm place sowing tomato seeds, cultivates, grows tomato seedling.
6. cultivation method according to claim 5, it is characterized in that, described Glomus mosseae solid fungicide is prepared by the following method: to cross the river sand of 2mm sieve for matrix, after high-temperature sterilization, place 10 ~ 14 days under nature, then basin is put into, every 2kg matrix inoculation 100-150g Glomus mosseae bacterium original seed, mix thoroughly, add the water of matrix weight 18 ~ 25%, sowing sorghum seeds, gathers in the crops the host plant that infected by Glomus mosseae and the matrix original seed containing Glomus mosseae spore, the outer mycelia of root after 5 ~ 6 months; Then sorghum field upper part is cut off, shred root segment, mix with matrix original seed, be i.e. obtained Glomus mosseae solid fungicide.
7. cultivation method according to claim 1, it is characterized in that, before tomato seedling is transplanted, in tomato seedling root system position inoculation phosphorus-solubilizing bacteria concrete steps be: transplant in tomato seedling and start for first 2 weeks, inoculate Pseudomonas alcaligenes liquid bacterial agent 2 ~ 4 times at tomato seedling root system position, each every strain tomato seedling inoculum concentration is 10
3~ 10
5individual Pseudomonas alcaligenes.
8. cultivation method according to claim 7, it is characterized in that, described Pseudomonas alcaligenes liquid bacterial agent is prepared by the following method: contain in the wheat bran leaching juice of 5.0 ~ 16.0g calcium carbonate at every 1000ml, access Pseudomonas alcaligenes, cultivate under normal condition, obtain Pseudomonas alcaligenes liquid bacterial agent.
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| CN108235912A (en) * | 2018-01-11 | 2018-07-03 | 云南省林业科学院 | It is a kind of using coffee industrial wood waste as the seedling medium and preparation method of raw material |
| CN113079970A (en) * | 2021-04-07 | 2021-07-09 | 山东省寿光蔬菜产业集团有限公司 | Method for cultivating tomato seedlings capable of resisting root-knot nematode |
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