CN105309289B - A kind of cultural method for improving the anti-root-knot nematode of northern facility tomato - Google Patents
A kind of cultural method for improving the anti-root-knot nematode of northern facility tomato Download PDFInfo
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- CN105309289B CN105309289B CN201510665935.6A CN201510665935A CN105309289B CN 105309289 B CN105309289 B CN 105309289B CN 201510665935 A CN201510665935 A CN 201510665935A CN 105309289 B CN105309289 B CN 105309289B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a kind of cultural method for improving the anti-root-knot nematode of northern facility tomato.It includes：A, the preparation of seedling medium：The seedling medium is based on parts by volume, including 1 part of 24 parts of rice husk, 13 parts of fermentation poultry dung, 12 parts of river sand and perlite, is uniformly mixed, and after sterilizing, seedling medium is made；B, the Arbuscular Mycorrhizal Fungi fungi on seedling medium, forms mycorhiza tomato Inoculant, then sows tomato seeds on this mycorhiza tomato Inoculant, cultivates, grows tomato seedling；C, before tomato seedling transplanting, in tomato seedling root system position inoculation phosphorus-solubilizing bacteria, transplanting, tomato seedling is transplanted together with mycorhiza tomato Inoculant, earthing, cultivation management is carried out to tomato seedling.The method of the present invention, technique is simple, easy to operation, thus is easy to carry out batch production tomato seedling production.
Description
Technical field：
The invention belongs to agricultural production and microbe application field, and in particular to one kind improves the anti-root knot of northern facility tomato
The cultural method of nematode.
Background technology：
Tomato is one of main industrial crops in China, is the guarantee of increasing peasant income.Facilities vegetable tomato intensive degree
Higher, tomato rhizosphere soil continuous cropping obstacle phenomenon getting worse, particularly root-knot nematode is spread unchecked.Tomato in China planting soil passes at present
Disease occurs seriously, and continuous cropping causes tomato planting soil quality to decline, and every year because of the root-knot nematode underproduction 30%, accounts for tomato planting face
Long-pending more than 40%, wherein with the influence of root-knot nematode during northern district facility tomato planting, it is intensive to restrict tomato
Production.The facilities vegetable plantation intensive degree area higher in Shandong, root-knot nematode continuous cropping obstacle phenomenon is serious, tomato production by
Restricted to serious.In order to improve the anti-root-knot nematode of tomato in agricultural production, production at present is upper mainly to be carried out using stewing canopy mode,
And tomato cultivation by setting seedlings is main planting type, there are larger contradiction with tomato planting production for tomato soil continuous cropping obstacle.
Research at present it has been shown that Arbuscular Mycorrhizal Fungi fungi is not only able to improve the vegetable plant such as tomato to element absorptions such as phosphorus,
At the same time can reduce pathogenic microorganism etc. disease influence, caused wide academic interest.But due to mycorrhizal fungi
Cannot direct cultured in vitro, seriously constrain application of the mycorrhizal fungi on tomato, at present by mycorrhizal fungi and tomato inoculation educate
Seedling is the preferred manner of tomato seedling, but mycorrhizal fungi is not very prominent in big Tanaka effect performance, shows that mycorhiza is true
Bacterium individual contributions effect in rhizosphere microorganism state environment is relatively low, it is necessary to other microorganism collective effects.Tomato is matrixing to be educated
Seedling has been the important key link of tomato production, and matrixing nursery measure is that tomato seedling cultivates an important ring.
The content of the invention：
The object of the present invention is to provide a kind of method is simple, cost is relatively low, it can not only reduce root-knot nematode and tomato is invaded
Dye, moreover it is possible to promote tomato growth, improve the cultivation of the anti-root-knot nematode of raising northern facility tomato of the anti-root-knot nematode ability of tomato
Method.
Then the present invention cultivates mycorrhizal fungi and tomato seedling symbiosis first by the preparation of seedling medium, then by mycorhiza
Tomato seedling transplanting cultivation, tomato seedling transplanting before, using rhizosphere phosphorus-solubilizing bacteria liquid bacterial agent be inoculated with so that Mycorrhizal tomato with
Phosphorus-solubilizing bacteria establishes symbiosis, the tomato vegetative reproduction stage regulate and control rhizosphere microorganism environment, can promote Mycorrhizal tomato with
Phosphorus-solubilizing bacteria establishes symbiosis, improves the colonization status of mycorrhizal fungi, is greatly improved the anti-root-knot nematode ability of tomato, reduces root
Tie lines worm infects tomato, tomato growth is improved, so as to fulfill the purpose of the present invention.
The cultural method of the anti-root-knot nematode of raising northern facility tomato of the present invention, it is characterised in that comprise the following steps：
A, the preparation of seedling medium：The seedling medium is based on parts by volume, including 2-4 parts of rice husk, fermentation poultry dung 1-3
1 part of part, 1-2 parts of river sand and perlite, are uniformly mixed, and (under the conditions of 121 DEG C, sterilize 40-60 minutes, taking-up is cooled to after sterilizing
30-40 DEG C), seedling medium is made；
B, the Arbuscular Mycorrhizal Fungi fungi on seedling medium, forms mycorhiza tomato Inoculant, then in this mycorhiza tomato Inoculant
Upper sowing tomato seeds, culture, grow tomato seedling；
C, before tomato seedling transplanting, in tomato seedling root system position inoculation phosphorus-solubilizing bacteria, transplanting, connect together with mycorhiza tomato
Tomato seedling is transplanted in kind agent together.Then to the tomato seedling earthing after transplanting, cultivation management is carried out to tomato seedling.
The mycorrhizal fungi is preferably Glomus mosseae (Glomus mosseae).
The phosphorus-solubilizing bacteria is preferably Pseudomonas alcaligenes (Pseudomonas alcaligenes).
The Arbuscular Mycorrhizal Fungi fungi on seedling medium, forms mycorhiza tomato Inoculant, is then connect in this mycorhiza tomato
Tomato seeds are sowed in kind agent, are cultivated, growing tomato seedling is preferably：Glomus mosseae is inoculated with seedling medium, is formed
Mycorhiza tomato Inoculant, then sows tomato seeds on this mycorhiza tomato Inoculant, and culture, grows tomato seedling, it is ensured that
Single plant tomato seedling contains 90~150 Glomus mosseae spores.
It is preferred that with every gram of Glomus mosseae solid fungicide containing 40 Glomus mosseae spores with seedling medium according to volume
Than for 1：25~35 ratio is uniformly mixed, and is formed mycorhiza tomato Inoculant, is subsequently filled into foam hole seedlings nursing plate, in cave
Tomato seeds are sowed at 2~3cm of middle mycorhiza tomato Inoculant lower face, culture, grows tomato seedling.
It is preferred that the Glomus mosseae solid fungicide is prepared by the following method：To cross the river sand of 2mm sieves as base
Matter, after high-temperature sterilization, places 10~14 days, is then placed in basin under natural conditions, per 2kg matrix inoculation 100-150g Mosess
Sacculus mould original seed, is mixed thoroughly, adds the water of matrix weight 18~25%, sows sorghum seeds, and harvest is by Moses's ball after five months
The mould host plant infected of capsule and the matrix original seed containing mycelia outside Glomus mosseae spore, root；Then sorghum aerial part
Cut off, shred root segment, mixed with matrix original seed, that is, Glomus mosseae solid fungicide is made.Every gram of Glomus mosseae solid fungicide
Contain 40 Glomus mosseae spores.
Before tomato seedling transplanting, at tomato seedling root system position, inoculation phosphorus-solubilizing bacteria is preferably：2 before tomato seedling transplanting
Week, the inoculation Pseudomonas alcaligenes liquid bacterial agent 2~4 times at tomato seedling root system position, every time every plant of tomato seedling inoculation
Measure as 103~105A Pseudomonas alcaligenes.
The Pseudomonas alcaligenes liquid bacterial agent is prepared by the following method：Contain 5.0 in every 1000ml~
In the wheat bran leaching juice of 16.0g calcium carbonate, Pseudomonas alcaligenes is accessed, is cultivated under normal condition, obtains Pseudomonas alcaligenes liquid
Microbial inoculum.
Tomato seedling (seedling medium and the Arbuscular Mycorrhizal Fungi fungi and the tomato of phosphorus-solubilizing bacteria culture of the method according to the invention culture
Seedling), verified by crop field and pot experiment, root-knot nematode can be significantly reduced Tomato Root System is infected, strengthen the anti-root knot of tomato
Nematode ability, mitigates cell membrane lipid extent of peroxidation, stabilizes membrane structure, reduce cell membrane permeability, significantly improve
Tomato mitigates pathogenic microorganism injury, has to northern facility tomato production important to the anti-infection ability of root-knot nematode after transplanting
Meaning.Compared with conventional tomato nursery, the present invention can improve tomato biological amount 10-15%, improve or significantly improve tomato production
Amount, can reach tomato root-knot eelworm prevention effect 38-56%, tomato production reaches the basic of high yield and high quality using this method
It is required that.
By tomato seedling Arbuscular Mycorrhizal Fungi fungi, make the Mycorrhizal of tomato seedling process, Mycorrhizal tomato seedling inoculates
Phosphorus-solubilizing bacteria, makes Tomato Root System establish symbiosis with mycorrhizal fungi, phosphorus-solubilizing bacteria, not only improves rhizosphere environment, improves bacterium
Mycorrhiza fungi is infected, and improves the anti-root-knot nematode ability of tomato, other extraneous pathogenic microorganisms of enhancing Tomato Root System resistance infect,
The effect of reaching control tomato root-knot eelworm, so as to effectively improve the ability of the anti-root-knot nematode of tomato.The method of the present invention, technique
Simply, it is easy to operation, thus be easy to carry out batch production tomato seedling production.
The Pseudomonas alcaligenes (Pseudomonas alcaligenes) and Glomus mosseae used in the present invention
(Glomus mosseae) is disclosed bacterial strain in the prior art.Wherein Pseudomonas alcaligenes (Pseudomonas
Alcaligenes) record (bibliography is disclosed in the literature：Lin Zhang&Jiequn Fan&Xiaodong Ding&
Xinhua He&Fusuo Zhang&Gu Feng.Hyphosphere interactions between an arbuscular
mycorrhizal fungus and a phosphate solubilizing bacterium promote phytate
mineralization in soil)；Glomus mosseae (Glomus mosseae) discloses record (with reference to text in the literature
Offer：Xiaodong Ding&Xinhua Sui&Fang Wang&Junhua Gao&Xinhua He&Fusuo Zhang&
Juncheng Yang&Gu Feng.Synergistic interactions between Glomus mosseae and
Bradyrhizobium japonicum in enhancing proton release from nodules and
hyphae.Mycorrhiza,2012,22:51-58), above-mentioned two microorganisms the applicant also holds, and guarantee can be at this
There is provided from patent application day in 20 years to the public.
Embodiment：
Following embodiments are to further explanation of the invention, rather than limitation of the present invention.
Embodiment 1
1st, the preparation of tomato seedling substrate：According to volume ratio takes 3 parts of rice husks, (prepared by compost fermentation method for 2 parts of fermentation poultry dungs：
Divulge information, high land, by with bedding and padding chicken manure accumulation it is in heaps, outside is closed with mud；Fermentation time is summer 15
It or so, 50 days or so winter), 2 parts of particle diameters be 1mm river sand and 1 part of particle diameter be 1mm perlite (rice husk：Fermentation poultry dung：
River sand：Perlite=3:2:2:1), be uniformly mixed, formed mixture, after by mixture under the conditions of 121 DEG C, sterilize 50 minutes,
Taking-up is cooled to 40 DEG C, and seedling medium is made, spare.
2nd, the preparation method of Glomus mosseae solid fungicide：It is (every after high-temperature sterilization using the river sand of 2mm sieves excessively as matrix
Secondary 121 DEG C of high pressure steam sterilization 45min), place 10 days under natural conditions, then fill two kilograms and be put into plastic tub, be inoculated with
100g Glomus mosseae bacterium original seeds, are mixed thoroughly, add the tap water of matrix weight 25%, sow sorghum seeds, keep normal growth,
The host plant that is infected by Glomus mosseae bacterium is can obtain after six months and containing mycelia outside Glomus mosseae bacterium spore, root
Matrix original seed；Sorghum overground part sub-cut is gone, shreds root segment, and with the matrix containing mycelia outside Glomus mosseae bacterium spore, root
Mix, that is, Glomus mosseae solid fungicide is made.Every gram of Glomus mosseae solid fungicide contains 40 Glomus mosseae spores.
3rd, by this Glomus mosseae solid fungicide according to volume ratio 1：25 are uniformly mixed with seedling medium, and obtain mycorhiza kind
Eggplant Inoculant；Tomato seedling is carried out with substrate seedling method, mycorhiza tomato Inoculant is filled to the hole of foam hole seedlings nursing plate
In, tomato seeds are then sowed at 2~3cm of mycorhiza tomato Inoculant lower face, culture, grows tomato seedling.
4th, the preparation and inoculation of Pseudomonas alcaligenes liquid bacterial agent：Culture medium：Contain 5g carbon in per 1000ml wheat bran leaching juice
Sour calcium (CaCO3), pH 6.5；Pseudomonas alcaligenes is inoculated into the above-mentioned culture mediums of 1000ml, 200rpm, 28 DEG C, culture 24
Hour, Pseudomonas alcaligenes liquid bacterial agent is obtained, the bacterium number of every milliliter of bacterium solution reaches 1.0 × 109A/mL.Moved in tomato seedling
Start within 2 weeks before cultivation, at tomato seedling root system position, Pseudomonas alcaligenes liquid bacterial agent, each every plant of tomato seedling connect twice for inoculation
Kind amount is 7 × 104A Pseudomonas alcaligenes, during transplanting, tomato seedling is transplanted together with mycorhiza tomato Inoculant, earthing, so
Afterwards to tomato seedling conventional cultivation management.
Effect of inoculation：
According to the method described above, in the Field trial demonstration test in the multiple tomato places of production in Shandong Province, the results are shown in Table 1 for it.
As it can be seen from table 1 relative to control (conventional substrate tomato seedling), Glomus mosseae is inoculated with tomato according to the present embodiment
Tomato chlorophyll and superoxide dismutase content can be significantly improved with Pseudomonas alcaligenes, so as to alleviate cell membrane lipid peroxidating
Degree, stabilizes membrane structure, reduces cell membrane permeability, can significantly reduce tomato seedling early stage root knot line after transplanting
The injury of worm, it is significant to the tomato production of Shandong District.The present invention method can improve tomato biological amount 13~
18%, therefore tomato yield 12~18% slightly can be improved or significantly improve, tomato production reaches the basic demand of high yield and high quality.
28-47% can be reached to the root knot nematode control effect of tomato using this method.
Table 1：Matrixing nursery inoculation Glomus mosseae and Pseudomonas alcaligenes are to Development of Tomato and root-knot nematode
The effect of prevention
Embodiment 2：
1st, the preparation of tomato seedling substrate：The river that 2 parts of rice husks, 1 part of fermentation poultry dung, 1 part of particle diameter are 1mm is taken according to volume ratio
Perlite (the rice husk that husky and 1 part of particle diameter is 1mm：Fermentation poultry dung：River sand：Perlite=2:1:1:1), it is uniformly mixed, is formed mixed
Compound, after by mixture under the conditions of 121 DEG C, sterilize 40 minutes, taking-up be cooled to 30 DEG C, be made seedling medium, it is spare.
2nd, the preparation method of Glomus mosseae solid fungicide：It is (every after high-temperature sterilization using the river sand of 2mm sieves excessively as matrix
Secondary 121 DEG C of high pressure steam sterilization 45min), place 14 days under natural conditions, then fill two kilograms and be put into basin, inoculation 100g rubs
Western sacculus mould original seed, is mixed thoroughly, adds the tap water of matrix weight 20%, sows the seed of sorghum and shallot, keeps normal raw
It is long, the host plant that is infected by Glomus mosseae bacterium is can obtain after six months and containing bacterium outside Glomus mosseae bacterium spore, root
The matrix of silk；Sorghum and shallot aerial part are cut off, shred root segment, and with containing mycelia outside Glomus mosseae bacterium spore, root
Matrix mix, that is, Glomus mosseae solid fungicide is made.Every gram of Glomus mosseae solid fungicide contains 40 Glomus mosseaes
Spore.
2nd, by this Glomus mosseae solid fungicide according to volume ratio 1：35 are uniformly mixed with seedling medium, and obtain mycorhiza kind
Eggplant Inoculant.Tomato seedling is carried out with substrate seedling method, mycorhiza tomato Inoculant is filled to the hole of foam hole seedlings nursing plate
In, tomato seeds are then sowed at 2~3cm of mycorhiza tomato Inoculant lower face, culture, grows tomato seedling.
3rd, the inoculation of Pseudomonas alcaligenes liquid bacterial agent：Prepare culture medium：Contain 6g carbonic acid in per 1000ml wheat bran leaching juice
Calcium (CaCO3), pH 7.5；Pseudomonas alcaligenes is inoculated into the above-mentioned culture mediums of 1000ml, 200rpm, 28 DEG C, culture 24 is small
When, Pseudomonas alcaligenes liquid bacterial agent is obtained, the bacterium number of every milliliter of bacterium solution reaches 1.0 × 109A/mL.Transplanted in tomato seedling
Start within first 2 weeks, 4 Pseudomonas alcaligenes liquid bacterial agents are inoculated with tomato seedling root system position, every time every plant of tomato seedling inoculation
Measure as 1 × 105A Pseudomonas alcaligenes, during transplanting, tomato seedling is transplanted together with mycorhiza tomato Inoculant, earthing, then
To tomato seedling conventional cultivation management.
Effect of inoculation：
According to the method described above, verify by field experiment, relative to control (not being inoculated with Glomus mosseae and phosphorus-solubilizing bacteria), press
Glomus mosseae and phosphorus-solubilizing bacteria raising tomato biological amount 19.4% are inoculated with tomato according to what the present embodiment was implemented, significantly improves tobacco leaf
Chlorophyll and superoxide dismutase content, so as to alleviate cell membrane lipid extent of peroxidation, stabilize membrane structure, reduce
Cell membrane permeability, can significantly improve the anti-root-knot nematode ability of tomato seedling after transplanting.Root using this method to tomato
The prevention effect of tie lines worm ability can reach 28-52%.
Embodiment 3：
1st, the preparation of tomato seedling substrate：The river that 4 parts of rice husks, 3 parts of fermentation poultry dungs, 1 part of particle diameter are 1mm is taken according to volume ratio
Perlite (the rice husk that husky and 1 part of particle diameter is 1mm：Fermentation poultry dung：River sand：Perlite=4:3:1:1), it is uniformly mixed, is formed mixed
Compound, after by mixture under the conditions of 121 DEG C, sterilize 40 minutes, taking-up be cooled to 30 DEG C, be made seedling medium, it is spare.
2nd, the preparation method of Glomus mosseae solid fungicide：It is (every after high-temperature sterilization using the river sand of 2mm sieves excessively as matrix
Secondary 121 DEG C of high pressure steam sterilization 45min), place 10 days, then taken in two kilograms of loading basins under natural conditions, inoculation 150g rubs
Western sacculus mould original seed, is mixed thoroughly, the tap water of addition matrix weight 18%, sowing sorghum seeds, holding normal growth, six months
It can obtain the host plant infected by Glomus mosseae bacterium and the matrix containing mycelia outside Glomus mosseae bacterium spore, root afterwards；
Sorghum overground part sub-cut is gone, shreds root segment, and is mixed with the matrix containing mycelia outside Glomus mosseae bacterium spore, root, that is, is made
Obtain Glomus mosseae solid fungicide.Every gram of Glomus mosseae solid fungicide contains 40 Glomus mosseae spores.
3rd, by Glomus mosseae solid fungicide according to volume ratio 1：30 are uniformly mixed with seedling medium, and obtain mycorhiza tomato
Inoculant.Tomato seedling is carried out with substrate seedling method, mycorhiza tomato Inoculant is filled into the hole of foam hole seedlings nursing plate,
Ensure that single plant contains 90~150 mycorrhizal fungal spores in seedlings nursing plate hole, then mycorhiza tomato Inoculant lower face 2~
Tomato seeds, the thinning after seed sprouting, per Kong Liumiao 1 are sowed at 3cm.
4th, it is inoculated with Pseudomonas alcaligenes solid fungicide：The preparation of Pseudomonas alcaligenes liquid bacterial agent：Culture medium：Per 1000ml
Contain 16g calcium carbonate (CaCO in wheat bran leaching juice3), pH 6.0；Pseudomonas alcaligenes is inoculated into the above-mentioned culture mediums of 1000ml,
200rpm, when culture 24 is small, obtains Pseudomonas alcaligenes liquid bacterial agent, the bacterium number of every milliliter of bacterium solution reaches 0.5 × 10 by 28 DEG C9
A/mL.Start within 2 weeks before tomato seedling transplanting, at tomato seedling root system position, respectively alkali vacation unit cell is once produced in inoculation within the 7th, 14 day
Bacteria liquid microbial inoculum, every plant of tomato seedling inoculum concentration is 1 × 10 every time3A Pseudomonas alcaligenes, during transplanting, connects together with mycorhiza tomato
Tomato seedling, earthing, then to tomato seedling conventional cultivation management are transplanted in kind agent together.
Effect of inoculation：
According to the method described above, verified by field experiment, (conventional seedbed system matrix and Moses's sacculus is not inoculated with relative to control
Mould and phosphorus-solubilizing bacteria), according to what the present embodiment was implemented tomato biological amount can be improved in tomato inoculation Glomus mosseae and phosphorus-solubilizing bacteria
10%, chlorophyll in tobacco leaf and superoxide dismutase content are significantly improved, so as to alleviate cell membrane lipid extent of peroxidation, is stabilized
Membrane structure, reduces cell membrane permeability, plays the role of obviously in terms of anti-root-knot nematode.Using this method pair
The root knot nematode control effect of tomato can reach 31-54%.
Claims (6)
1. a kind of cultural method for improving the anti-root-knot nematode of northern facility tomato, it is characterised in that comprise the following steps：
A, the preparation of seedling medium：The seedling medium is based on parts by volume, including 2-4 parts of rice husk, 1-3 parts of fermentation poultry dung, river
Husky 1-2 parts and 1 part of perlite, are uniformly mixed, and after sterilizing, seedling medium is made；
B, the Arbuscular Mycorrhizal Fungi fungi on seedling medium, forms mycorhiza tomato Inoculant, is then broadcast on this mycorhiza tomato Inoculant
Kind tomato seeds, culture, grows tomato seedling；
C, before tomato seedling transplanting, in tomato seedling root system position inoculation phosphorus-solubilizing bacteria, transplanting, together with mycorhiza tomato Inoculant
Tomato seedling is transplanted together, and earthing, cultivation management is carried out to tomato seedling；
The mycorrhizal fungi is Glomus mosseae (Glomus mosseae)；
The phosphorus-solubilizing bacteria is Pseudomonas alcaligenes (Pseudomonas alcaligenes).
2. cultural method according to claim 1, it is characterised in that the Arbuscular Mycorrhizal Fungi fungi on seedling medium,
Mycorhiza tomato Inoculant is formed, then sows tomato seeds on this mycorhiza tomato Inoculant, is cultivated, grows tomato seedling tool
Body step is：Glomus mosseae is inoculated with seedling medium, mycorhiza tomato Inoculant is formed, then in this mycorhiza tomato Inoculant
Upper sowing tomato seeds, culture, grow tomato seedling, it is ensured that single plant tomato seedling contains 90~150 Glomus mosseae spores.
3. cultural method according to claim 1, it is characterised in that with every gram of Moses containing 40 Glomus mosseae spores
The mould solid fungicide of sacculus is 1 according to volume ratio with seedling medium：25~35 ratio is uniformly mixed, and forms the inoculation of mycorhiza tomato
Agent, is subsequently filled into foam hole seedlings nursing plate, sows tomato species at 2~3cm of mycorhiza tomato Inoculant lower face in cave
Son, culture, grows tomato seedling.
4. cultural method according to claim 3, it is characterised in that the Glomus mosseae solid fungicide be by with
Prepared by lower section method：Using the river sand for crossing 2mm sieves as matrix, after high-temperature sterilization, place 10~14 days under natural conditions, Ran Houfang
Enter in basin, per 2kg matrix inoculation 100-150g Glomus mosseae bacterium original seeds, mix thoroughly, add the water of matrix weight 18~25%, broadcast
Kind of sorghum seeds, the host plant that is infected by Glomus mosseae is harvested after 5~6 months and containing Glomus mosseae spore, root outside
The matrix original seed of mycelia；Then sorghum overground part sub-cut is gone, shreds root segment, mixed with matrix original seed, that is, Moses's sacculus is made
Mould solid fungicide.
5. cultural method according to claim 1, it is characterised in that before tomato seedling transplanting, in tomato seedling root system
Position inoculation phosphorus-solubilizing bacteria concretely comprises the following steps：Start within 2 weeks before tomato seedling transplanting, it is false that production alkali is inoculated with tomato seedling root system position
Monad liquid bacterial agent 2~4 times, every plant of tomato seedling inoculum concentration is 10 every time3~105A Pseudomonas alcaligenes.
6. cultural method according to claim 5, it is characterised in that the Pseudomonas alcaligenes liquid bacterial agent is to pass through
Prepared by following methods：In every 1000ml contains the wheat bran leaching juice of 5.0~16.0g calcium carbonate, Pseudomonas alcaligenes is accessed, often
Cultivated under the conditions of rule, obtain Pseudomonas alcaligenes liquid bacterial agent.
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| CN113079970B (en) * | 2021-04-07 | 2022-08-12 | 山东省寿光蔬菜产业集团有限公司 | Method for cultivating tomato seedlings capable of resisting root-knot nematodes |
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| JPH06205666A (en) * | 1993-01-08 | 1994-07-26 | Idemitsu Kosan Co Ltd | Method for multiplying va mycorrhizal fungus |
| NZ248768A (en) * | 1993-09-24 | 1996-12-20 | Sieber Agricultural Res Ltd | Liquid fertiliser; liquid plant nutrient and at least one introduced fungus, micro-organism and/or reproductive material therefor |
| CN102503699A (en) * | 2011-11-17 | 2012-06-20 | 郭现广 | Lysozyme soil sterilization deep ploughing-free anti-hardening root cultivation and protection microorganism fertilizer |
| CN102613054B (en) * | 2012-04-09 | 2013-03-27 | 广东省生态环境与土壤研究所 | Method for improving cold and disease resistance of tobacco |
| CN103271095B (en) * | 2013-04-11 | 2015-01-21 | 山西省农业科学院果树研究所 | Composite microbial agent for preventing and treating fruit tree replant disease and disease prevention method thereof |
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