CN114504545B - Polypeptide and plant extraction composition for removing wrinkles and application - Google Patents
Polypeptide and plant extraction composition for removing wrinkles and application Download PDFInfo
- Publication number
- CN114504545B CN114504545B CN202210226095.3A CN202210226095A CN114504545B CN 114504545 B CN114504545 B CN 114504545B CN 202210226095 A CN202210226095 A CN 202210226095A CN 114504545 B CN114504545 B CN 114504545B
- Authority
- CN
- China
- Prior art keywords
- parts
- extract
- polypeptide
- skin
- magnolia sieboldii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 86
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 84
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 84
- 239000000203 mixture Substances 0.000 title claims abstract description 48
- 230000037303 wrinkles Effects 0.000 title claims abstract description 40
- 238000000605 extraction Methods 0.000 title claims description 23
- 239000000284 extract Substances 0.000 claims abstract description 88
- 241001673954 Magnolia sieboldii Species 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 150000001875 compounds Chemical class 0.000 claims abstract description 21
- 239000000706 filtrate Substances 0.000 claims abstract description 21
- 241000620196 Arthrospira maxima Species 0.000 claims abstract description 19
- 244000236658 Paeonia lactiflora Species 0.000 claims abstract description 19
- 235000008598 Paeonia lactiflora Nutrition 0.000 claims abstract description 19
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 19
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims abstract description 18
- 235000004279 alanine Nutrition 0.000 claims abstract description 18
- 229940059958 centella asiatica extract Drugs 0.000 claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 239000000047 product Substances 0.000 claims abstract description 18
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims abstract description 17
- 239000004472 Lysine Substances 0.000 claims abstract description 17
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 17
- CAHKINHBCWCHCF-UHFFFAOYSA-N N-acetyltyrosine Chemical compound CC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 CAHKINHBCWCHCF-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229960004308 acetylcysteine Drugs 0.000 claims abstract description 17
- 239000000419 plant extract Substances 0.000 claims abstract description 17
- 239000004475 Arginine Substances 0.000 claims abstract description 15
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 239000002537 cosmetic Substances 0.000 claims abstract description 9
- ANZUDYZHSVGBRF-UHFFFAOYSA-N 3-ethylnonane-1,2,3-triol Chemical compound CCCCCCC(O)(CC)C(O)CO ANZUDYZHSVGBRF-UHFFFAOYSA-N 0.000 claims description 17
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 17
- RGUVUPQQFXCJFC-UHFFFAOYSA-N n-hydroxyoctanamide Chemical compound CCCCCCCC(=O)NO RGUVUPQQFXCJFC-UHFFFAOYSA-N 0.000 claims description 17
- 235000005206 Hibiscus Nutrition 0.000 claims description 8
- 235000007185 Hibiscus lunariifolius Nutrition 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000003480 eluent Substances 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- AJLNZWYOJAWBCR-OOPVGHQCSA-N (4s)-4-acetamido-5-[[(2s)-1-[[(2s)-1-[[(2s)-5-amino-1-[[(2s)-1-[[(2s)-1-amino-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-car Chemical compound OC(=O)CC[C@H](NC(C)=O)C(=C)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(N)=O AJLNZWYOJAWBCR-OOPVGHQCSA-N 0.000 claims description 4
- 229940095094 acetyl hexapeptide-8 Drugs 0.000 claims description 4
- 108010006338 acetyl-glutamyl-glutamyl-methionyl-glutaminyl-arginyl-argininamide Proteins 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- KNFLNGRLKALWRF-LDXSYGEZSA-N CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)CC1=CC=CC=C1 Chemical compound CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)CC1=CC=CC=C1 KNFLNGRLKALWRF-LDXSYGEZSA-N 0.000 claims description 3
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 244000284380 Hibiscus rosa sinensis Species 0.000 claims 1
- 102000008186 Collagen Human genes 0.000 abstract description 23
- 108010035532 Collagen Proteins 0.000 abstract description 23
- 229920001436 collagen Polymers 0.000 abstract description 23
- 102000016942 Elastin Human genes 0.000 abstract description 13
- 108010014258 Elastin Proteins 0.000 abstract description 13
- 229920002549 elastin Polymers 0.000 abstract description 13
- 230000037394 skin elasticity Effects 0.000 abstract description 10
- 230000015572 biosynthetic process Effects 0.000 abstract description 7
- 230000001502 supplementing effect Effects 0.000 abstract description 5
- 238000003786 synthesis reaction Methods 0.000 abstract description 5
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 230000002000 scavenging effect Effects 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 50
- 238000012360 testing method Methods 0.000 description 19
- 230000000052 comparative effect Effects 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 13
- 241000235346 Schizosaccharomyces Species 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 241000218033 Hibiscus Species 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 239000000835 fiber Substances 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 210000004207 dermis Anatomy 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 210000004177 elastic tissue Anatomy 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000222640 Polyporus Species 0.000 description 2
- 230000002292 Radical scavenging effect Effects 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 2
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- RRJOMESUBQAYOA-BZSNNMDCSA-N (2S)-2-[[(2S)-2-[[2-[[(2S)-2-acetamido-6-aminohexanoyl]amino]acetyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-6-aminohexanamide Chemical compound NCCCC[C@H](NC(=O)C)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(N)=O)CC1=CN=CN1 RRJOMESUBQAYOA-BZSNNMDCSA-N 0.000 description 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010006895 Cachexia Diseases 0.000 description 1
- 102000005600 Cathepsins Human genes 0.000 description 1
- 108010084457 Cathepsins Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 244000130592 Hibiscus syriacus Species 0.000 description 1
- 235000018081 Hibiscus syriacus Nutrition 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 102000004183 Synaptosomal-Associated Protein 25 Human genes 0.000 description 1
- 108010057722 Synaptosomal-Associated Protein 25 Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000000744 eyelid Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 210000000216 zygoma Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a polypeptide and plant extract composition for removing wrinkles and application thereof, and aims to provide a composition for supplementing elastin to skin in time, promoting synthesis of collagen, scavenging free radicals, supplementing water required by skin, increasing skin elasticity and removing wrinkles; the technical key points include: 1-5 parts of polypeptide compound, 1-6 parts of paeonia lactiflora extract, 0.5-4 parts of centella asiatica extract, 1.2-3.5 parts of fermentation product filtrate of two-split yeast, 2-7 parts of spirulina maxima extract, 3-7 parts of magnolia sieboldii extract, 0.5-1.5 parts of saccharomycete polypeptide, 0.5-1.5 parts of arginine/lysine polypeptide, 0.5-1 part of alanine, 0.5-1 part of acetylcysteine and 0.5-1 part of acetyltyrosine; belongs to the technical field of cosmetics.
Description
Technical Field
The invention discloses a composition, in particular relates to a polypeptide and plant extraction composition for removing wrinkles and application thereof, and belongs to the technical field of cosmetics.
Background
The skin is covered on the body surface, and is sequentially an epidermis, a dermis and a subcutaneous layer from outside to inside. The dermis is generally 1-2 mm thick and is differentiated from mesoderm, and is divided into a papillary layer and a reticular layer, and no obvious limit exists between the papillary layer and the reticular layer. The dermis is a bulge with numerous papillae embedded in the deep surface of the epidermis, which is connected to the superficial fascia by connective tissue fiber bundles.
Wrinkles are a "natural enemy" of women, but everyone cannot prevent the passage of years. Starting from about 35 years old, the metabolism of the person begins to slow down, the sebaceous and sweat gland functions slowly decline, the internal fibre reserves supporting the skin surface gradually deplete, causing the tension inside the skin to decrease outwards and the local areas to subside, so that wrinkles are produced and become more apparent. Particularly, in the periocular region with thinner skin and less fiber reserve, problems such as fish tail, eyelid relaxation and the like are more likely to occur.
The main reason is that the skin is influenced by the external environment to form free radicals, and collagen and active substances in normal cell membrane tissues are destroyed by the free radicals. Resulting in a gradual decrease in collagen content of the dermis layer of the skin. The reticular supporting body can also become thicker and harder and lose elasticity, and when the elasticity and the water retention degree of the dermis layer are reduced, the skin can lose elasticity and become thinner and aged; the epidermis forms loose wrinkles.
The dermis layer mainly contains two proteins, namely collagen and elastin. Collagen is a biopolymer, which is present in skin, bones, tendons, blood vessels and other connective tissues, and occupies 70% of the skin, forming an "elastic net" of the skin, which can moisturize, inhibit wrinkles, reduce pigmentation, make the skin on the face look elastic and shiny, and is as tough as a gel, but is essentially a protein. The protein of the whole body of the human body has more or less 20-25% of collagen.
However, as the age increases, collagen begins to run off, just as when the sponge begins to be in a dehydrated state, the sponge is drier as the water runs off, the sponge is drier and the water is not locked, and cycle by cycle, the cachexia circulation is formed, so that serious unbalance of water and oil of the skin is caused, and the first stage of skin aging is caused; following a significant loss of moisture, the skin elasticity becomes weaker and, over time, the collagen network begins to break, and when it begins to break, there is no way to support the skin, the face will begin to appear fine lines at the corners of the eyes, and french lines, eventually forming true wrinkles.
Elastin is an important component of skin composition, and only 5% of elastin is in the skin, but is a key for exciting generation of skin collagen and elastic fibers, 70% of collagen in the skin determines softness and plumpness of the skin, and 90% of elastic fibers are composed of elastin.
The elasticity of the skin depends on the elastic fiber of the inner layer of the skin, which is equivalent to innumerable elastic fiber webs supporting skin tissues. Whereas 90% of the spandex is composed of elastin, the amount of elastin determines the elasticity of the skin and whether wrinkles occur. However, elastin in the skin is not constant, and elastin is reduced, which is an important cause of skin aging.
Therefore, in order to reduce wrinkles in skin, delay aging, make skin tender, plump, elastic, compact and glossy, it is necessary to supplement moisture, collagen and elastin required by skin in time, and remove free radicals.
Disclosure of Invention
Aiming at the defects, the invention aims to provide a polypeptide and plant extract composition for removing wrinkles, which has the advantages that all the components are synergistic, elastin can be timely supplemented to skin, collagen synthesis is promoted, free radicals are eliminated, moisture required by the skin is supplemented, skin elasticity is increased, and the wrinkles are removed.
For this purpose, the first technical solution provided in the present application is as follows:
a polypeptide and plant extract composition for reducing wrinkles comprises the following components in parts by weight: 1-5 parts of polypeptide compound, 1-6 parts of paeonia lactiflora extract, 0.5-4 parts of centella asiatica extract, 1.2-3.5 parts of fermentation product filtrate of two-split yeast, 2-7 parts of spirulina maxima extract, 3-7 parts of magnolia sieboldii extract, 0.5-1.5 parts of saccharomycete polypeptide, 0.5-1.5 parts of arginine/lysine polypeptide, 0.5-1 part of alanine, 0.5-1 part of acetylcysteine and 0.5-1 part of acetyltyrosine.
Further, the polypeptide and plant extract composition for reducing wrinkles further comprises 1-4 parts of the extract of the hibiscus syriacus flower and 1-3 parts of the extract of Jiang Huagen.
Further, the polypeptide and plant extract composition for reducing wrinkles further comprises 1-2 parts of a flos mori extract.
Further, the polypeptide and plant extract composition for reducing wrinkles further comprises 0.1-0.3 part of octanoyl hydroxamic acid and 0.5-2 parts of ethylhexyl glycerol.
Further, the polypeptide and plant extract composition for reducing wrinkles further comprises 10-15 parts of butanediol and 10-20 parts of water.
Further, the polypeptide compound for reducing wrinkles and the plant extract composition consists of acetyl hexapeptide-8, tripeptide-1, tetrapeptide-1 and nonapeptide-1 in equal mass ratio.
Further, a polypeptide and plant extract composition for reducing wrinkles as described above, wherein the magnolia sieboldii extract is prepared by the following method:
1) Cleaning radix Magnolia sieboldii, drying, pulverizing, and sieving with 50-100 mesh sieve to obtain radix Magnolia sieboldii powder;
2) Adding the magnolia sieboldii obtained in the step 1) into an extraction tank, adding an extraction solvent with the volume of 8-12 times, putting into an ultrasonic vibration instrument, treating for 45-60min under the ultrasonic wave of 50-55kHZ, filtering, repeating for 3 times, combining the 3 filtrates, and concentrating under reduced pressure to 1/4-1/5 of the original volume;
3) And (3) loading the concentrated solution in the step (2) on an HP-20 macroporous adsorption resin column, eluting with water for 3-4 times, discarding the eluent, eluting with 75% ethanol water solution, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the magnolia sieboldii extract.
Further, the above-mentioned polypeptide and plant extract composition for removing wrinkles, wherein the extraction solvent is ultrapure water.
The second technical scheme provided by the invention is as follows:
a cosmetic comprises polypeptide and plant extract composition for removing wrinkles, wherein the polypeptide and plant extract composition are 3-10% of the total mass of the cosmetic.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the polypeptide complex compounding and plant extraction composition provided by the technical scheme of the invention, acetyl hexapeptide-8 participates in competition of SNAP-25 at the site of the foam-melting complex, so that the formation of the complex is influenced, and the muscle contraction is weakened, and the formation of wrinkles is prevented; the plant extract composition and tripeptide, acetyl tetrapeptide-1 and pentapeptide-3 can further stimulate and promote the generation of collagen, fibronectin, elastin, hyaluronic acid, etc.; inhibiting glycosyl, reducing the problem of disordered and loose collagen fibers, and recovering elasticity; the skin is restored to be youthful and smooth.
(2) According to the technical scheme provided by the invention, the peptide compound is compounded with the paeonia lactiflora extract, the centella asiatica extract and the fermentation product filtrate of the two-way yeast, the spirulina maxima extract, the magnolia sieboldii extract, the saccharomycete polypeptides, the amino acid/lysine polypeptides, the alanine, the acetylcysteine and the acetyltyrosine, so that the inhibition of elastase and glucuronidase is further increased, the effects of promoting the activity of cathepsin and the activity of fiber cells, eliminating free radicals and inhibiting the generation of nitrogen oxides are achieved, the degradation of collagen fibers can be slowed down, the renewal of cells is stimulated, the service life of the cells is prolonged, and the skin elasticity can be maintained.
(3) According to the technical scheme provided by the invention, the peptide compound is compounded with the paeonia lactiflora extract, the centella asiatica extract and the fermentation product filtrate of the two-split yeast, the spirulina maxima extract, the magnolia sieboldii extract, the saccharomycete polypeptides, the amino acid/lysine polypeptides, the alanine, the acetylcysteine and the acetyltyrosine, so that the softening of the horny layer can be effectively promoted, the hydration function of cells can be increased, the water content of the horny layer can be improved, the moisture retention property is good, the deep water supplementing is realized, and the skin is moist and has high elasticity.
(4) According to the technical scheme provided by the invention, the armature hibiscus flower extract, the Jiang Huagen extract and the spring hibiscus flower extract are further compounded, so that the skin antioxidation capability can be improved, and meanwhile, the components are synergistic to synthesize collagen and enhance the cell proliferation speed; the butanediol and water are used as solvents, and the octanoyl hydroxamic acid and the ethylhexyl glycerol are used as preservatives, so that the preservative is favorable for maintaining and adding, and the moisturizing performance is further improved.
(5) The technical scheme provided by the invention can effectively supplement iodine and various mineral substances, vitamins, proteins and amino acids, accelerate metabolism, maintain skin activity, increase the skin antioxidant property, resist inflammation and the like, thereby achieving the effects of tightening the skin, reducing wrinkles, increasing elasticity and delaying aging.
(6) According to the technical scheme provided by the invention, the magnolia sieboldii extract is extracted by combining ultrasonic waves with the adsorption resin column, so that the extraction efficiency is high, phenols and magnaline of the magnolia sieboldii extract are effectively maintained, the oxidation resistance and anti-aging performance of the magnolia sieboldii extract are improved, the degradation of collagen fibers is slowed down, and skin elasticity is maintained.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. For process parameters or conditions not specifically noted, reference may be made to conventional techniques.
The raw materials used in the invention are purchased through commercial sources.
Example 1
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 3g of polypeptide compound, 1g of paeonia lactiflora extract, 4g of centella asiatica extract, 2.5g of fermentation product filtrate of two-split yeast, 3g of spirulina maxima extract, 4g of magnolia sieboldii extract, 0.5g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.5g of alanine, 1g of acetylcysteine, 0.6g of acetyltyrosine, 10g of butanediol, 20g of water, 0.1g of octanoyl hydroxamic acid and 2g of ethylhexyl glycerol.
Example 2
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 5g of polypeptide compound, 4g of paeonia lactiflora extract, 1g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces, 7g of spirulina maxima extract, 5g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1.5g of arginine/lysine polypeptide, 1g of alanine, 0.5g of acetylcysteine, 0.5g of acetyltyrosine, 15g of butanediol, 15g of water, 0.15g of octanoyl hydroxamic acid and 1.5g of ethylhexyl glycerol.
Example 3
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 2g of polypeptide compound, 6g of paeonia lactiflora extract, 0.5g of centella asiatica extract, 3.5g of fermentation product filtrate of two-split yeast, 2g of spirulina maxima extract, 3g of magnolia sieboldii extract, 1.5g of saccharomycetes polypeptide, 0.5g of arginine/lysine polypeptide, 0.7g of alanine, 0.8g of acetylcysteine, 0.7g of acetyltyrosine, 15g of butanediol, 10g of water, 0.2g of octanoyl hydroxamic acid and 1.8g of ethylhexyl glycerol.
Example 4
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 1g of polypeptide compound, 2g of paeonia lactiflora extract, 2g of centella asiatica extract, 1.2g of fermentation product filtrate of schizosaccharomyces, 4g of spirulina maxima extract, 7g of magnolia sieboldii extract, 1.2g of saccharomycetes polypeptide, 1.2g of arginine/lysine polypeptide, 0.6g of alanine, 0.7g of acetylcysteine, 0.8g of acetyltyrosine, 12g of butanediol, 13g of water, 0.25g of octanoylhydroxamic acid and 1.25g of ethylhexyl glycerol.
Example 5
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
Example 6
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 1g of hibiscus flower extract, 3g of Jiang Huagen extract, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
Example 7
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 4g of hibiscus flower extract, 1g of Jiang Huagen extract, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
Example 8
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 2g of hibiscus flower extract, 2g of Jiang Huagen extract, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
Example 9
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of two-split yeast, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 2g of hibiscus flower extract, 2g of Jiang Huagen extract, 1g of polyporus schneiderianus extract, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
Example 10
The invention provides a polypeptide and plant extraction composition for removing wrinkles, which comprises the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of two-split yeast, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 2g of hibiscus flower extract, 2g of Jiang Huagen extract, 2g of polyporus schneiderianus extract, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
The polypeptide complexes described in examples 1 to 10 consist of acetyl hexapeptide-8, tripeptide-1, tetrapeptide-1, pentapeptide-3, nonapeptide-1 in equal mass ratios.
The magnolia sieboldii extracts of examples 1-10 were prepared by the following method:
1) Cleaning radix Magnolia sieboldii, drying, pulverizing, and sieving with 50-100 mesh sieve to obtain radix Magnolia sieboldii powder;
2) Adding the magnolia sieboldii obtained in the step 1) into an extraction tank, adding an extraction solvent with the volume of 8-12 times, putting into an ultrasonic vibration instrument, treating for 45-60min under the ultrasonic wave of 50-55kHZ, filtering, repeating for 3 times, combining the 3 filtrates, and concentrating under reduced pressure to 1/4-1/5 of the original volume;
3) And (3) loading the concentrated solution in the step (2) on an HP-20 macroporous adsorption resin column, eluting with water for 3-4 times, discarding the eluent, eluting with 75% ethanol water solution, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the magnolia sieboldii extract.
In order to demonstrate the effect of the present invention, comparative experimental examples of the present application are given below:
comparative example 1
A composition consists of the following components in parts by weight: 2g of polypeptide compound, 7g of paeonia lactiflora extract, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
Comparative example 2
A composition consists of the following components in parts by weight: 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces cerevisiae, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 3g of saccharomycetes polypeptides, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
Comparative example 3
A composition consists of the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces cerevisiae, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract, 1g of saccharomycetes polypeptide, 3.25g of alanine, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
The procedure for the preparation of magnolia sieboldii extract in the comparative example was the same as in the examples.
Comparative example 4
A composition consists of the following components in parts by weight: 2g of polypeptide compound, 3g of paeonia lactiflora extract, 2g of centella asiatica extract, 2g of fermentation product filtrate of schizosaccharomyces cerevisiae, 4g of spirulina maxima extract, 4g of magnolia sieboldii extract (commercially available), 1g of saccharomycetes polypeptide, 1g of arginine/lysine polypeptide, 0.75g of alanine, 0.75g of acetylcysteine, 0.75g of acetyltyrosine, 13g of butanediol, 12g of water, 0.2g of octanoyl hydroxamic acid and 1g of ethylhexyl glycerol.
The preparation method of the composition comprises the following steps: the components are weighed according to any one group of weight numbers in examples 1-10 and comparative examples 1-4, and are stirred and mixed uniformly at normal temperature to obtain the composite material.
In order to better illustrate the advantages of the present invention, the following provides experimental effects of the technical solution provided by the present invention:
experiment 1 Effect on collagen Synthesis
Test sample: examples 1, 5, 8, 10, compositions of comparative examples 1-4.
The testing method comprises the following steps: the contents of collagens I, III and IV of fibroblasts are measured by an ELISA method, the fibroblasts are digested by trypsin and transferred into 96-well culture plates, edge wells are filled with sterile phosphate buffer solution, the culture is continued in an incubator until the cells are completely adherent to the wall, 20 mu L/well of the composition sample solution of examples 1, 5, 8 and 10 and comparative examples 1-4 are sequentially added by using a microscale sample adding device, 0.01MPBS with the same volume is added in a control group, the contents of collagens I, III and IV are measured according to a kit instruction after 48 hours of adding samples, 3 compound wells are used as a control group, and the synthesis amount of the collagens is 100%. The test results are shown in Table 1.
Table 1 amounts of collagen synthesized in I, III and IV types
Experiment 2 moisture effect experiment
The specific test method is as follows:
reference standard: reference is made to the QB/T4256-2011 guidelines for evaluation of moisturizing efficacy of cosmetics.
The test person: 40 healthy volunteers were selected, 30-40 years old, randomly grouped, 5 per group. The samples were applied twice daily in the morning and evening. During the experiment, the subject was unable to apply any other cosmetics at the experimental site.
Test conditions: the skin moisture meter Corneometer CM825 by capacitance method has the testing temperature of 20-22 ℃ and the humidity of 40-60%.
The test principle is as follows: the capacitance method is used for measuring the moisture content of the skin horny layer of a human body, the dielectric constants of the skin horny layer and other substances are obviously different, the capacitance value of the skin is different according to the difference of the moisture content of the skin horny layer, and the parameters can represent the moisture content (MMV) of the skin.
Test sample: the experimental groups were compositions provided in examples 1, 5, 8, and 10, and the compositions of comparative examples 1 to 4 were added to the same emulsion matrix in an amount of 5wt% each, and samples 1, 5, 8, and 10 to be tested, and control samples 1 to 4 were obtained in order.
The testing method comprises the following steps: each region is fixedly measured at three points according to a certain sequence, and the average value of the three points is obtained. Then the sample to be tested and the control sample are smeared on the tested part (according to the mg/cm of (2.0+/-0.1)) 2 Is used singlySecondary coating), measuring skin moisture content of the tested part by using a probe at 0h, 2h, 4h and 8h, calculating average values of each group at corresponding time points, and calculating a skin moisture content increase rate according to the formula:
skin moisture content increase% = (MMVt-MMV 0 )/MMV 0 ×100％
MMV 0 -pre-application skin MMV
MMV t Skin MMV t period after application
The results are shown in Table 2.
TABLE 2 skin moisture content increase Rate
Experiment 3 skin elasticity test
Test sample: the experimental groups were compositions provided in examples 1, 5, 8, and 10, and the compositions of comparative examples 1 to 4 were added to the same emulsion matrix in an amount of 5wt% each, and samples 1, 5, 8, and 10 to be tested, and control samples 1 to 4 were obtained in order.
The test person: 40 healthy volunteers were selected, 30-40 years old, randomly grouped, 5 per group. The samples were applied twice daily in the morning and evening. During the experiment, the subject was unable to apply any other cosmetics at the experimental site.
Skin elasticity test: skin conditions at weeks 0, 2 and 4 were measured using a skin elastometer Cutometer MPA580, respectively, with the test sites being the cheekbones, and the probe was repeatedly measured 3 times in the same area under measurement, and the average was taken.
Skin elasticity increase rate W% = (NT-N0)/n0×100%
N0-skin elasticity test value before application.
Nt—t period elasticity test value after application.
The test results are shown in table 3:
TABLE 3 skin elasticity increasing rate
Experiment 4, DPPH radical scavenging test
0.2mmol/L DPPH solution is prepared by 50% ethanol and stored in a dark place. The experimental groups were prepared by preparing 0.2mg/mL of the tested solution from the compositions provided in examples 1, 5, 8 and 10 and the compositions of comparative examples 1-4, measuring the absorbance A at 517nm, measuring 3 times in parallel, taking an average value, and the experimental results are shown in Table 4.
Wherein: a is that i Absorbance for reaction after addition of the test substance;
A j the absorbance of the sample solution is the self absorbance;
A 0 the blank value was obtained without adding the test solution.
TABLE 4 DPPH radical scavenging results
| Group of | DPPH clearance (%) |
| Example 1 | 89.3060 |
| Example 5 | 91.6941 |
| Example 8 | 92.6047 |
| Example 10 | 95.9928 |
| Comparative example 1 | 84.1564 |
| Comparative example 2 | 82.6040 |
| Comparative example 3 | 83.6713 |
| Comparative example 4 | 76.5964 |
As is apparent from tables 1 to 4, the composition provided by the invention has the advantages of synergistic components, timely supplementing elastin to skin, promoting synthesis of collagen, scavenging free radicals, supplementing water required by skin, increasing skin elasticity and reducing wrinkles.
Claims (3)
1. A polypeptide and plant extract composition for reducing wrinkles, which is characterized by comprising the following components in parts by weight: 1-5 parts of polypeptide compound, 1-6 parts of paeonia lactiflora extract, 0.5-4 parts of centella asiatica extract, 1.2-3.5 parts of fermentation product filtrate of two-split yeast, 2-7 parts of spirulina maxima extract, 3-7 parts of magnolia sieboldii extract, 0.5-1.5 parts of saccharomycete polypeptide, 0.5-1.5 parts of arginine/lysine polypeptide, 0.5-1 part of alanine, 0.5-1 part of acetylcysteine, 0.5-1 part of acetyltyrosine, 1-4 parts of harness hibiscus flower extract, 1-3 parts of Jiang Huagen extract and 1-2 parts of telescoping flower extract; 0.1-0.3 part of octanoyl hydroxamic acid and 0.5-2 parts of ethylhexyl glycerol; 10-15 parts of butanediol and 10-20 parts of water;
the polypeptide compound consists of acetyl hexapeptide-8, tripeptide-1, tetrapeptide-1, pentapeptide-3 and nonapeptide-1 in equal mass ratio;
the magnolia sieboldii extract is prepared by the following method:
1) Cleaning radix Magnolia sieboldii, drying, pulverizing, and sieving with 50-100 mesh sieve to obtain radix Magnolia sieboldii powder;
2) Adding the magnolia sieboldii obtained in the step 1) into an extraction tank, adding an extraction solvent with the volume of 8-12 times, putting into an ultrasonic vibration instrument, treating for 45-60min under the ultrasonic wave of 50-55kHZ, filtering, repeating for 3 times, combining the 3 filtrates, and concentrating under reduced pressure to 1/4-1/5 of the original volume;
3) And (3) loading the concentrated solution in the step (2) on an HP-20 macroporous adsorption resin column, eluting with water for 3-4 times, discarding the eluent, eluting with 75% ethanol water solution, recovering the eluent, and concentrating under reduced pressure to dryness to obtain the magnolia sieboldii extract.
2. The polypeptide and plant extract composition for reducing wrinkles according to claim 1, wherein said extraction solvent is ultrapure water.
3. A cosmetic comprising the polypeptide for reducing wrinkles and the plant extract composition of claim 1, wherein the polypeptide for reducing wrinkles and the plant extract composition account for 3-10% of the total mass of the cosmetic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210226095.3A CN114504545B (en) | 2022-03-08 | 2022-03-08 | Polypeptide and plant extraction composition for removing wrinkles and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210226095.3A CN114504545B (en) | 2022-03-08 | 2022-03-08 | Polypeptide and plant extraction composition for removing wrinkles and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114504545A CN114504545A (en) | 2022-05-17 |
| CN114504545B true CN114504545B (en) | 2024-01-23 |
Family
ID=81554656
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210226095.3A Active CN114504545B (en) | 2022-03-08 | 2022-03-08 | Polypeptide and plant extraction composition for removing wrinkles and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114504545B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115040467B (en) * | 2022-07-29 | 2024-05-03 | 哈尔滨敷尔佳科技股份有限公司 | Composition for relieving and resisting allergy, preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108324628A (en) * | 2018-05-18 | 2018-07-27 | 广东盛美化妆品有限公司 | A kind of Essence of the root extract containing Aplotaxis auriculata and preparation method thereof |
| CN108524357A (en) * | 2018-05-18 | 2018-09-14 | 广东盛美化妆品有限公司 | A kind of fresh and alive beautiful fine flour frost of the root extract containing Aplotaxis auriculata and preparation method thereof |
-
2022
- 2022-03-08 CN CN202210226095.3A patent/CN114504545B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108324628A (en) * | 2018-05-18 | 2018-07-27 | 广东盛美化妆品有限公司 | A kind of Essence of the root extract containing Aplotaxis auriculata and preparation method thereof |
| CN108524357A (en) * | 2018-05-18 | 2018-09-14 | 广东盛美化妆品有限公司 | A kind of fresh and alive beautiful fine flour frost of the root extract containing Aplotaxis auriculata and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114504545A (en) | 2022-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6620419B1 (en) | Cosmetic or dermopharmaceutical use of peptides for healing, hydrating and improving skin appearance during natural or induced ageing (heliodermia, pollution) | |
| CN113616566B (en) | Skin care essence for tightening skin and preparation method thereof | |
| CN112043621B (en) | Preservative-free polypeptide composition with anti-aging effect | |
| CN111773130A (en) | Hydroxypropyl tetrahydropyrane triol compound and preparation method and application thereof | |
| CN110833516B (en) | Polypeptide composition with moisturizing effect | |
| CN110496067A (en) | A kind of anti-wrinkle composition and its preparation method and application | |
| CN110664648A (en) | Caviar anti-aging composition | |
| CN106420472A (en) | Multifunctional anti-wrinkle skincare product composition and preparation method thereof | |
| CN114504545B (en) | Polypeptide and plant extraction composition for removing wrinkles and application | |
| CN112043619B (en) | Peptide composition with repairing effect and preparation method and application thereof | |
| CN114344188A (en) | Hair loss prevention and hair growth promoting composition and preparation method thereof | |
| CN114053168A (en) | Anti-aging composition and cosmetic composition | |
| CN111035590A (en) | Micromolecule polypeptide youth essence and preparation method thereof | |
| CN110151629B (en) | Anti-aging composition for promoting collagen synthesis and preparation method thereof | |
| CN114588061A (en) | Anti-aging and wrinkle-removing composition, preparation method thereof and skin care product | |
| CN110585111A (en) | Anti-aging cosmetic additive and preparation method thereof | |
| CN109846798B (en) | Composition for tightening skin and cosmetic containing same | |
| CN113057926A (en) | A skin care additive with wrinkle removing and antiaging effects, and its preparation method | |
| CN116999378A (en) | Tightening and skin-brightening composition containing super-living pearl polypeptide mother liquor and preparation method thereof | |
| CN110882177A (en) | Skin repair oligopeptide composition and preparation method thereof | |
| CN114712285B (en) | Composition with anti-wrinkle effect and preparation method and application thereof | |
| CN110585079A (en) | Radiation-resistant composition containing food-borne peptide and radiation-resistant emulsion thereof | |
| CN113730289B (en) | Eye and lip care composition with multiple repairing effects and application thereof | |
| CN114948803A (en) | Anti-aging essence and preparation method thereof | |
| CN107802554A (en) | Essence containing stem cell secretory factors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |