CN114477462A - Clean algae biological population domestication method for reducing algae density in water body and application thereof - Google Patents
Clean algae biological population domestication method for reducing algae density in water body and application thereof Download PDFInfo
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- CN114477462A CN114477462A CN202210080171.4A CN202210080171A CN114477462A CN 114477462 A CN114477462 A CN 114477462A CN 202210080171 A CN202210080171 A CN 202210080171A CN 114477462 A CN114477462 A CN 114477462A
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
Abstract
The invention provides a clean algae biological population domestication method for reducing the density of algae in a water body and application thereof, and the clean algae biological population domestication method comprises the steps of preparing a domestication culture pond which is cleaned and exposed to the sun; filtering water into the domestication and cultivation pond by adopting bolting silk; applying stable manure when the water inlet volume reaches half of the volume of the domesticated and cultured pond body, stirring, continuously feeding water to a set water level after stirring is completed, standing for a plurality of days, and fishing out floaters on the water surface; the method comprises the steps of sequentially carrying out introduction, domestication cultivation and screening in each domestication cultivation pool to obtain the algae capable of fast grazing the aquatic body, wherein the first domestication cultivation pool introduces an unacclimated and cultivated clean algae biological population, and the next domestication cultivation pool introduces the clean algae biological population obtained by domestication cultivation in the previous domestication cultivation pool.
Description
Technical Field
The invention relates to the technical field of clean algae biological population, algae control and ecological restoration, in particular to a clean algae biological population domestication method for reducing the density of algae in a water body and application thereof.
Background
Algae are a group of eukaryotes of the protist kingdom. Mainly aquatic, has no vascular bundle, and can perform photosynthesis. The size of the single cell flagellates with the size of 1 micron and the large brown algae with the size of 60 meters are different. Algae can be composed of one or a few cells, and many cells aggregate into a tissue-like architecture. The filaments may or may not be branched, some of the algae being unicellular dinoflagellates, others aggregating into populations. The pinus genus of green algae is formed by interweaving and winding numerous branch filaments, and the filament shapes and functions are different at different positions.
The transparency of the water body is reduced due to the large amount of algae, the photosynthesis of submerged plants is limited, the concentration of dissolved oxygen in the water body is reduced, and the survival of invertebrates and fishes is inhibited. The explosive proliferation of dominant algae can also produce a large amount of harmful gas and toxin, so that the water body is discolored and deteriorated, the diversity and stability of aquatic organisms are reduced, and the aquatic ecosystem is damaged. Meanwhile, the algae give off fishy smell and gather on the water surface in large quantity, which seriously affects the production and life of surrounding residents.
The existing methods for inhibiting algae in water basically depend on physical algae inhibiting technology, chemical algae inhibiting technology and biological algae inhibiting technology. The physical algae inhibiting technology has the defects of time consumption, high cost, difficult operation and the like, and is not easy to implement generally and on a large scale; the chemical algae inhibiting technology is a traditional algae treating method, and the principle is that specific medicines are sprinkled to a water body, the specific components in the medicines act on algae cells, so that the algae cells lose activity and die, the algae killing speed is high, but the algae cannot be treated fundamentally, the medicine property disappears, and the algae is easy to relapse; the biological algae inhibiting technology applies artificially cultured microbial inoculum with extremely strong activity to algae water to enable bacteria to propagate in water in a large quantity, and inhibits the growth and the propagation of the algae by means of competing nutrition and space environment with the algae or secreting bacteriostatic (algae) substances and the like, but the biological algae inhibiting technology has weak algae inhibiting capacity and can only be used in slight water of the algae, if the algae is serious, the biological algae inhibiting technology is basically ineffective, and simultaneously the algae cannot be treated fundamentally, and when the algae inhibiting microbial inoculum is weakened, the algae can be erupted again. Therefore, the clean algae biological population domestication method for reducing the density of the algae in the water body and the application thereof are provided.
Therefore, there is a need to provide a clean algae population domestication method for reducing the density of algae in water and an application thereof to solve the technical problems.
Disclosure of Invention
In order to solve the technical problems, the invention provides a clean algae biological population domestication method for reducing the density of algae in a water body and application thereof, which are used for solving the defects of time consumption, high cost, difficult operation and the like of the existing physical algae inhibiting technology and are not easy to implement generally and on a large scale; the chemical algae inhibiting technology cannot radically treat the algae, the medicine property disappears, and the algae is easy to relapse; the biological algae inhibiting technology has weak algae inhibiting capacity and can be used only in slight water body with algae.
The invention provides a clean algae biological population domestication method for reducing the density of algae in a water body, which comprises the following operation steps:
the method comprises the following steps: preparing at least seven domesticated culture ponds which have the depth of one meter and are subjected to cleaning and insolation treatment;
step two: filtering water into the domestication and cultivation pond prepared in the step one by adopting bolting silk;
step three: applying stable manure when the water inlet volume reaches half of the volume of the domesticated and cultured pond body, stirring, continuously feeding water to a set water level after stirring is completed, standing for a plurality of days, and fishing out floaters on the water surface;
step four: sequentially carrying out introduction, domestication cultivation and screening in each domestication cultivation pond to obtain algae capable of quickly grazing water body, wherein the clean algae biological population which is not domesticated and cultivated is introduced into the first domestication cultivation pond, and the clean algae biological population obtained by domestication and cultivation in the last domestication cultivation pond is introduced into the next domestication cultivation pond;
step five: step four, the induced algae-purifying biological population is plankton of herbivorous phytoplankton, wherein the plankton is specifically daphnia trunk, daphnia magna and rotifer;
step six: step four, domesticating and cultivating, namely putting 1L of blue algae water/m into the domesticating and cultivating pond3(pond water) in which the density of algae is 1X 1010And (2) per liter.
Step seven: and the screening in the fourth step comprises the steps of screening 20-30% of dominant species in the clean algae biological population domesticated and cultured in the first domesticated and cultured pond, and introducing the dominant species to the next domesticated and cultured pond until the last domesticated and cultured pond is obtained in sequence.
Preferably, the domesticated cultivation pond adopts a cement field or an earth pond.
Preferably, the mesh number of the bolting silk in the step one is not less than 120 meshes.
Preferably, the manure in the third step is 1.2-1.5 kg/m3(manure/tank volume).
Preferably, the proportion of the clean algae species is 100-200/m3(pool water).
Preferably, the period of single domestication cultivation is 10-15 days.
Preferably, the method also comprises monitoring the pH value of the water body, monitoring the dissolved oxygen of the water body and monitoring the water temperature in the operation process, wherein the specific pH value is 6-9, the dissolved oxygen of the water body is more than 3.2mg/L, and the water temperature is 18-28 ℃.
Compared with the related technology, the clean algae biological population domestication method for reducing the density of algae in the water body has the following beneficial effects:
the clean algae biological population domesticated and cultured by the invention has strong capacity of removing the algae density in the water body, obvious domestication and culture effect and obvious capacity improvement of reducing the algae density of the clean algae biological population.
The invention also provides application of the clean algae population obtained by the clean algae population domestication method for reducing the density of the algae in the water body in water body remediation.
Detailed Description
The present invention will be further described with reference to the following embodiments.
Preparing an acclimatization and cultivation pond: firstly, not less than 7 domestication and cultivation ponds with the depth of about 1 meter are prepared, the domestication and cultivation ponds can adopt cement ponds or soil ponds, cleaning and insolation treatment are needed before the domestication and cultivation ponds are used, and a large amount of pathogens, harmful organism spores and ova exist in the ponds which are not cleaned and subjected to insolation treatment, so that potential threats are caused to the healthy growth of planktonic organism populations.
Water inflow: the inlet water is filtered by adopting a bolting-silk (the mesh number of the bolting-silk is not less than 120 meshes) so as to prevent harmful microorganisms from being introduced into the inlet water.
Fertilizing water: applying stable manure when the water injection in the domestication and cultivation pond reaches about half of the volume of the pond body, wherein the nutrient content of the water body without the added stable manure is lower, and the growth speed of the clean algae biological population is lower; when the concentration of nutrient substances is too high due to excessive addition of the manure, the growth of the clean algae biological population is inhibited; 1.2-1.5 kg/m of stable manure3The ratio of the manure to the volume of the tank body is applied and the mixture is properly stirred. After the manure is applied and stirred, continuously injecting water to the designed water level. Standing for 3-5 days after water injection, and removing the floating objects on the water surface to perform introduction.
Introduction: introducing clean algae biological populations (mainly containing phytoplankton of grazing phytoplankton such as daphnia rhinorrhoea, daphnia magna, rotifer and the like) into the No. 1 domestication and cultivation pool, and introducing 100-200 clean algae organisms per liter of pool water.
Domestication and cultivation of clean algae biological population: 1L of blue algae water (algae density: 1 × 10) is put into the No. 1 domestication and cultivation pond according to the water of each cubic pond every day10And (L) performing clean algae biological population domestication cultivation.
Screening: the clean algae biological population domesticated and cultured in the domesticated and culturing pool No. 1 for 10-15 days has strong capability of adapting to the blue algae water body and is robust, 20-30% of dominant species are screened in the clean algae biological population, and the clean algae biological population is introduced into the domesticated and culturing pool No. 2.
And (3) repeatedly and circularly introducing, domesticating and culturing and screening, sequentially performing the operations of introducing, domesticating and culturing and screening of the clean algae biological population in each domesticating and culturing pond, wherein the repeated circulation frequency is more than 7 times, and finally performing domestication and improvement to obtain the optimal clean algae biological population capable of quickly grazing aquatic algae and reducing the algae density.
The water temperature monitoring is required to be enhanced, the water temperature is kept between 18 ℃ and 28 ℃, the zooplankton is suitable for the temperature between 18 ℃ and 28 ℃, the life of the zooplankton population with overhigh temperature is shorter, and the zooplankton population is not suitable for the growth and the propagation of the clean algae population.
Monitoring the pH value of the water body is enhanced, and the pH value of the water body is maintained at 6-9; the pH value has close relation with the metabolism, reproduction, development and other life activities of the clean algae biological population. The pure algae biological population has a larger tolerance range, and neutral or slightly alkaline water is suitable for the life of most of the pure algae biological populations. When the pH value of the water body is less than 6 or more than 9, the breeding of most clean algae biological populations can be inhibited.
Enhancing the monitoring of the dissolved oxygen in the water body, and keeping the concentration of the dissolved oxygen above 3.2 mg/L; the low concentration of the dissolved oxygen in the water can inhibit the growth of part of clean algae biological population. Such as copepods, daphnia zerumbet, a significant number of individual deaths occur if the water dissolved oxygen concentration is below 3.2 mg/L.
Controlling the single domestication cultivation period to be 10-15 days; the average generation time of the clean algae biological population is 10-15 days, the domestication cultivation period is too short, a new generation of clean algae biological population is not reproduced, the clean algae biological population screened out in too long period is mostly in the aging period, and the activity is insufficient.
The clean algae biological population is introduced, domesticated, cultivated and screened, the repeated cycle times are more than 7 times, and the clean algae capacity of the clean algae biological population is obviously improved along with the increase of the repeated cycle times when the repeated cycle times are compared within 7 times.
Clean algae test for domesticating clean algae biota
The test is divided into 8 comparison groups, and the effect of domesticated clean algae flora on the reduction of the algae density in different domestication stages is observed.
1. Preparation of the test
(1) And (4) preparing TP standard solution.
Potassium dihydrogen phosphate (KH2PO4) was accurately weighed and dissolved in ultrapure water to prepare a 100mg/L (in terms of P) phosphate stock solution.
(2) And (4) preparing an ammonia nitrogen standard solution.
1) Putting high-grade pure ammonium chloride (NH4Cl) into a drying box, drying for 2h at 105-110 ℃ to constant weight, putting into a dryer, and cooling for 40min for use.
2) Accurately weighing ammonium chloride (NH4Cl) and dissolving in ultrapure water to prepare 100mg/L ammonia nitrogen stock solution.
(3) Preparing algae water body simulation liquid.
Will be preparedPhosphate stock solution, ammonia nitrogen stock solution and blue algae water (density of algae: 1 × 10)10one/L) was diluted with ultrapure water to prepare 8 sets of mock solutions, each set being 100L.
The ammonia nitrogen concentration of the simulated liquid is 8mg/L, the TP concentration is 0.5mg/L, and the simulation of the primary A tail water of the urban sewage treatment plant (pollutant discharge Standard of urban sewage treatment plant (GB 18918-.
The algal density of the simulant was: 1.5X 108And (4) simulating a severe water bloom water body.
2. Experimental observation
And respectively adding the clean algae biological populations which are not subjected to domestication cultivation and are subjected to different domestication cultivation stages into the prepared algae water body simulation liquid, wherein the number of the clean algae biological populations added in each group is 500, and counting the algae density of each group of simulation liquid every 24 hours.
3. Observation results
From experimental observations it can be seen that: (1) the more the domestication and cultivation period is completed, the stronger the capacity of the clean algae biological population for removing the algae density in the water body is, the obvious domestication and cultivation effect is achieved, and the capacity of the clean algae biological population for reducing the algae density is obviously improved. (2) The experimental group that completed 7 acclimatization cultivation cycles was able to density the algae within 168h as: 1.5X 108The volume/L of the algae water body (severe bloom water body) is reduced to 9.8 multiplied by 10 algae density6A slight bloom water body of one/L. (3) Compared with the clean algae biological population which is not domesticated and cultivated, the algae removal rate within 168h is improved from 18.4 percent to 93.46 percent after 7 domestication and cultivation periods are completed.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the illustrated orientations or positional relationships, and are only used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present invention or directly or indirectly applied to other related technical fields are included in the scope of the present invention.
Claims (8)
1. A clean algae biological population domestication method for reducing the density of algae in a water body is characterized by comprising the following operation steps:
the method comprises the following steps: preparing at least seven domesticated culture ponds which have the depth of one meter and are subjected to cleaning and insolation treatment;
step two: filtering water into the domestication and cultivation pond prepared in the step one by adopting bolting silk;
step three: applying stable manure when the water inlet volume reaches half of the volume of the domesticated and cultured pond body, stirring, continuously feeding water to a set water level after stirring is completed, standing for a plurality of days, and fishing out floaters on the water surface;
step four: sequentially carrying out introduction, domestication cultivation and screening in each domestication cultivation pool to obtain algae capable of fast grazing water body, wherein the first domestication cultivation pool introduces a clean algae biological population which is not domesticated and cultivated, and the next domestication cultivation pool introduces a clean algae biological population which is obtained by domestication and cultivation in the previous domestication cultivation pool;
step five: step four, the induced algae-cleaning biological population is plankton of herbivorous phytoplankton, wherein the plankton is specifically daphnia rhynchophylla, daphnia magna and rotifer;
step six: step four, domesticating and cultivating, namely putting 1L of blue algae water/m into the domesticating and cultivating pond3(pond water) with algae density of 1X 1010And (2) per liter.
Step seven: and the screening in the fourth step comprises the steps of screening 20-30% of dominant species in the clean algae biological population domesticated and cultured in the first domesticated and cultured pond, and introducing the dominant species to the next domesticated and cultured pond until the last domesticated and cultured pond is obtained in sequence.
2. The method for acclimatizing clean algae biological population for reducing the density of algae in water according to claim 1, wherein the acclimatizing and cultivating pond adopts a cement land or an earth pond.
3. The method for acclimatizing clean algae biological population for reducing the density of algae in water according to claim 1, wherein the mesh number of the bolting silk in the first step is not less than 120 meshes.
4. The clean algae biological population domestication method for reducing the density of algae in water body according to claim 1, characterized in that the manure in the third step is 1.2-1.5 kg/m3(manure/tank volume).
5. The method of claim 1, wherein the ratio of clean algae species is 100-200/m3(pond water).
6. The clean algae biological population domestication method for reducing the algae density in the water body according to claim 1, wherein the period of single domestication cultivation is 10-15 days.
7. The clean algae biological population domestication method for reducing the density of algae in water body according to claim 1, further comprising monitoring the pH value of the water body, monitoring the dissolved oxygen of the water body and monitoring the water temperature in the operation process, wherein the specific pH value is 6-9, the dissolved oxygen of the water body is more than 3.2mg/L, and the water temperature is 18-28 ℃.
8. Use of a clean algal bio-population obtained by the clean algal bio-population acclimation method for reducing algal density in a water body according to any one of claims 1 to 7 in water body remediation.
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| CN104585140A (en) * | 2015-01-19 | 2015-05-06 | 蓝志娟 | Domesticating method for daphnias and method for utilizing daphnias to perform ecological remediation on water containing drained sludge of sewage treatment plant |
| CN104787893A (en) * | 2015-03-31 | 2015-07-22 | 上海太和水环境科技发展有限公司 | Daphnia magna domestication method and application thereof |
| CN104986861A (en) * | 2015-06-19 | 2015-10-21 | 南京信息工程大学 | Underflow type artificial wetland plant arrangement method used for purifying sewage of thermal power plant |
| US20210403356A1 (en) * | 2019-10-25 | 2021-12-30 | Beijing Drainage Group Co., Ltd | Bacterium-Alga Coupled Sewage Treatment Device Based on Energy Recycling and Use Method Thereof |
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