CN114469834A

CN114469834A - Composition for inhibiting acne and preparation method and application thereof

Info

Publication number: CN114469834A
Application number: CN202111517687.2A
Authority: CN
Inventors: 张萍玲; 陈宇霞; 陈庆生
Original assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Current assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Priority date: 2021-12-13
Filing date: 2021-12-13
Publication date: 2022-05-13

Abstract

The invention belongs to the technical field of cosmetics, and discloses a composition for inhibiting acne, a preparation method and application thereof. The composition comprises the following raw material components: tortoise plastron, glabrous greenbrier rhizome, rehmannia root, raw licorice, cape jasmine fruit and honeysuckle flower. Through the mutual synergistic effect of the tortoise plastron, the glabrous greenbrier rhizome, the rehmannia root, the raw liquoric root, the gardenia and the honeysuckle, the composition provided by the invention is nontoxic, can inhibit the reproduction of propionibacterium acnes, inhibit the release of outer membrane vesicles of propionibacterium acnes, inhibit the secretion of skin grease, inhibit the spread of acne and improve the influence of acne on the face, and has the characteristics of safe raw materials, simple preparation method and easy industrial realization.

Description

Composition for inhibiting acne and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a composition for inhibiting acne, and a preparation method and application thereof.

Background

Acne is a chronic inflammatory disease of pilosebaceous tissue, is caused by a large amount of androgen-induced sebum, abnormal keratinization of pilosebaceous glands, deposit of propionibacterium acnes, secondary inflammation and other reasons, and clinically shows various types of skin rash such as acne, nodules, pustules, papules, cysts and the like. Severe acne can lead to persistent scarring, skin damage, pustules, post-inflammatory pigmentation in the patient, and can have a serious negative impact on the quality of life, particularly the psychological aspects of the patient. Therefore, it is crucial to prevent and treat acne in a timely manner.

At present, various medicines are applied to the clinical treatment of acne, such as tetracycline, macrolide antibacterial medicines, benzoyl peroxide, vitamin A acid medicines, hormones and the like are common, and the medicines have slow effect and are easy to cause side effects after long-term use, such as teratogenicity and depression of isotretinoin, drug resistance improvement caused by erythromycin antibacterial medicines, vomiting and diarrhea caused by tetracycline antibiotics and the like.

Therefore, there is a need to provide a product that is safe and can inhibit acne.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides a composition for inhibiting acne, a preparation method and application thereof, which can effectively inhibit acne.

In a first aspect, the present invention provides a composition for inhibiting acne, the composition comprising the following raw material components: tortoise plastron, glabrous greenbrier rhizome, rehmannia root, raw liquoric root, cape jasmine fruit and honeysuckle flower.

In the pharmacological action, the tortoise plastron is salty and sweet in taste and cold in nature, enters kidney, liver and heart channels, can nourish yin and tonify kidney, is sufficient in kidney yin, clears heart fire, and has the effects of expelling toxin, nourishing yin and beautifying by taking the tortoise plastron as a monarch and the rhizoma smilacis glabrae as a minister. The rehmannia root, the liquorice, the gardenia and the honeysuckle have the effects of clearing heat and removing toxicity, the honeysuckle and the gardenia have the effect of clearing heat, the liquorice has the effect of removing toxicity, the rehmannia root has the effect of nourishing yin, and the rehmannia root is used for assisting the monarch and minister to nourish yin, clear heat and remove toxicity.

Rhizoma Smilacis Glabrae is sweet and light in flavor, neutral in nature, and enters liver and stomach meridians, and has effects of removing toxic substance, eliminating dampness, dispelling pathogenic wind and relieving pain. The record of the herbal atlas indicates that the glabrous greenbrier rhizome has the function of applying sore toxin, and has good curative effect on acne skin lesions and the glabrous greenbrier rhizome both for oral administration and external use. Modern pharmacological studies show that astilbin, the main component of smilax glabra, may affect the level of androgen in the body, thus affecting sebum secretion and affecting the living environment of propionibacterium acnes.

The dried rehmannia root is sweet and bitter in taste and cold in nature, enters heart, liver and kidney channels, and has the effects of clearing heat, cooling blood, nourishing yin and promoting the production of body fluid. The dried rehmannia root can inhibit vascular endothelial inflammation and experimental synovial swelling inflammation of joints of rats.

The liquorice has sweet taste and neutral nature, enters heart, lung, spleen and stomach channels, and has the effects of tonifying spleen and qi, clearing away heat and toxic materials, eliminating phlegm and stopping cough, relieving spasm and pain and harmonizing the medicines. In Zhongqing gan Cao from Yao Hua Yi (pharmaceutical science), raw licorice root, with the actions of cooling and purging fire, mainly dispersing exterior pathogen, resolving swollen welling-abscess, relieving sore throat, removing all drug-toxin, removing heat accumulated in stomach and relieving pain in ureter, also has the actions of cooling and removing heat. Modern researches have proved that glycyrrhizin, the main active ingredient of licorice, has the functions of detoxification and anti-inflammation, and licoflavone also has the anti-inflammation function, and is beneficial to repairing damaged skin.

Gardenia is bitter in taste and cold in nature, and enters heart channel, lung channel and triple energizer channel, and has the effects of purging pathogenic fire, relieving restlessness, clearing heat, promoting urination, cooling blood, removing toxicity, relieving swelling and pain by external use and inhibiting various pathogenic bacteria.

The honeysuckle flower is sweet in taste and cold in nature, enters lung, heart and stomach meridians, and has the effects of clearing away heat and toxic materials and dispelling wind and heat. The honeysuckle contains honeysuckle glycoside, tannin, alkaloid and the like, and the pharmacological test proves that the honeysuckle has the inhibiting effect on various pathogenic bacteria and has stronger carbuncle-dispersing, swelling-dispersing, heat-clearing, detoxifying and inflammation-diminishing effects on carbuncle, furuncle, acute appendicitis and pulmonary abscess.

According to some embodiments of the invention, the tortoise plastron is a ventral or dorsal concha of a tortoise.

According to some embodiments of the invention, the composition comprises the following raw material components in parts by weight: 1-20 parts of tortoise plastron, 1-25 parts of glabrous greenbrier rhizome, 1-20 parts of radix rehmanniae, 1-20 parts of raw liquorice, 1-15 parts of cape jasmine and 1-15 parts of honeysuckle.

Preferably, the composition comprises the following raw material components in parts by weight: 15-20 parts of tortoise plastron, 15-20 parts of glabrous greenbrier rhizome, 8-15 parts of radix rehmanniae, 15-20 parts of raw liquorice, 10-15 parts of cape jasmine and 10-15 parts of honeysuckle.

Preferably, the composition comprises the following raw material components in parts by weight: 16-20 parts of tortoise plastron, 16-20 parts of glabrous greenbrier rhizome, 10-14 parts of radix rehmanniae, 17-20 parts of raw liquorice, 11-14 parts of cape jasmine fruit and 13-15 parts of honeysuckle.

According to some embodiments of the invention, the composition further comprises the following raw material components: radix Angelicae Dahuricae.

The angelica dahurica is pungent in flavor and warm in nature, enters the channels of the lung, the spleen and the stomach, and has the effects of dispelling wind and cold, dredging orifices and relieving pain, reducing swelling and expelling pus, eliminating dampness and stopping leukorrhagia and the like. The compendium of materia medica says that the dahurian angelica root can be used as face fat, has the functions of relieving fever, easing pain, resisting inflammation, detoxifying and expelling pus and the like, and can improve local blood circulation and promote metabolism of the skin.

According to some embodiments of the invention, the composition comprises the following raw material components in parts by weight: 1-20 parts of tortoise plastron, 1-25 parts of glabrous greenbrier rhizome, 1-20 parts of radix rehmanniae, 1-20 parts of raw liquorice, 1-15 parts of cape jasmine fruit, 1-15 parts of honeysuckle and 1-10 parts of dahurian angelica root.

Preferably, the composition comprises the following raw material components in parts by weight: 15-20 parts of tortoise plastron, 15-20 parts of glabrous greenbrier rhizome, 8-15 parts of radix rehmanniae, 15-20 parts of raw liquorice, 10-15 parts of cape jasmine fruit, 10-15 parts of honeysuckle and 5-9 parts of dahurian angelica root.

Preferably, the composition comprises the following raw material components in parts by weight: 16-20 parts of tortoise plastron, 16-20 parts of glabrous greenbrier rhizome, 10-14 parts of radix rehmanniae, 17-20 parts of raw liquorice, 11-14 parts of cape jasmine fruit, 13-15 parts of honeysuckle and 6-8 parts of dahurian angelica root.

A second aspect of the present invention provides a process for the preparation of the above composition, said process comprising the steps of: obtaining extracts of the raw material components; mixing the extracts of the raw material components to obtain the composition.

According to some embodiments of the invention, the extract obtained from each raw material component is: pulverizing carapax et Plastrum Testudinis, decocting, filtering, and concentrating to obtain carapax et Plastrum Testudinis extract; pulverizing the rest raw materials, extracting with solvent, and refluxing to obtain mixed extractive solution.

According to some embodiments of the invention, the solvent is at least one of water, methanol, ethanol; preferably, the solvent is a mixture of water and methanol or ethanol; preferably, the solvent is a mixture of water and ethanol; preferably, the solvent is ethanol water solution with the volume concentration of 60-70%.

According to some embodiments of the present invention, the extraction is microwave extraction, the frequency of the extraction is 2000-3000MHz, the power is 600-1000W, the temperature is 40-60 ℃, and the time is 20-60 min.

According to some embodiments of the invention, the method of preparing comprises the steps of:

(1) pulverizing carapax et Plastrum Testudinis, sieving with 100 mesh sieve, decocting with water for 2-5 times (each time for 0.5-3 hr), filtering the decoction, and vacuum concentrating in 60-90 deg.C water bath to obtain carapax et Plastrum Testudinis extract;

(2) pulverizing the rest raw materials, sieving with 100 mesh sieve, mixing, adding 60-70% ethanol solution, microwave extracting, and hot refluxing to obtain mixed extractive solution; the frequency of microwave extraction is 2000-3500MHz, and the power is 600-1000W; the extraction temperature is 35-45 deg.C, and the extraction time is 20-60 min; the reflux frequency is 3 times, and the reflux temperature is 70-90 ℃;

(3) mixing the carapax et Plastrum Testudinis extract and the mixed extractive solution, dissolving, filtering with 80-120 mesh filter cloth, vacuum concentrating the filtrate at 40-60 deg.C to 100g, and dissolving with 100g 1, 3-butanediol to obtain the composition.

A third aspect of the invention provides the use of a composition as described above in the preparation of a cosmetic product.

According to some embodiments of the invention, the composition is for use in the manufacture of a cosmetic product for inhibiting acne.

According to some embodiments of the invention, the composition is for use in the manufacture of a cosmetic for inhibiting the proliferation of propionibacterium acnes, inhibiting the release of outer membrane vesicles from propionibacterium acnes, and/or inhibiting the release of inflammatory factors.

In a fourth aspect, the present invention provides a cosmetic product comprising the composition described above.

According to some embodiments of the invention, the cosmetic is for inhibiting acne.

According to some embodiments of the invention, the cosmetic is for inhibiting the proliferation of propionibacterium acnes, inhibiting the release of outer membrane vesicles from propionibacterium acnes, and/or inhibiting the release of inflammatory factors.

According to some embodiments of the invention, the composition is added in an amount of 0.1% to 20% by weight of the total weight of the cosmetic product. Preferably, the composition is added in an amount of 0.1% to 10% by weight based on the total weight of the cosmetic.

According to some embodiments of the invention, the cosmetic is a gel, a cream, a mask, a lotion, a serum, or a lotion.

Therefore, the beneficial effects of the invention include:

1. the composition for inhibiting acne has no cytotoxicity by the mutual synergistic effect of tortoise plastron, glabrous greenbrier rhizome, rehmannia root, raw liquoric root, cape jasmine fruit and honeysuckle, and has the effects of inhibiting the reproduction of propionibacterium acnes, inhibiting the release of outer membrane vesicles of propionibacterium acnes and inhibiting the release of inflammatory factors;

2. the composition for inhibiting acne can inhibit the secretion of skin grease, improve mottle, wrinkles, textures, pores, purpura and red areas of the skin and reduce the white head, black head, pimple and cyst nodule of the face caused by the acne when being applied to the face.

3. The composition provided by the invention is safe in raw materials, simple in preparation method and easy to realize in industrialization.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions in the embodiments of the present invention are described clearly and completely below.

The technical scheme provided by the embodiment of the invention is described below.

Example 1

The present example provides a composition comprising the following raw material components: 16g of tortoise plastron, 20g of glabrous greenbrier rhizome, 14g of rehmannia root, 17g of raw liquorice, 12g of cape jasmine fruit, 13g of honeysuckle flower and 8g of dahurian angelica root.

The preparation method of the composition comprises the following steps: pulverizing carapax et Plastrum Testudinis, decocting in 160g water for 1 hr for 3 times, mixing decoctions, filtering with 100 mesh gauze, and vacuum concentrating at 70 deg.C in water bath to obtain carapax et Plastrum Testudinis extract; pulverizing the rest raw materials, sieving with 100 mesh sieve, mixing, adding into 1.4L 70% ethanol water solution, performing microwave extraction at 3000MHz and 800W for 30min at 40 deg.C, extracting at 80 deg.C under reflux for 40min, and extracting for 3 times to obtain mixed extractive solution; dissolving carapax et Plastrum Testudinis extract in the mixed extractive solution, and filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated under vacuum at 50 ℃ to 100g, and then 100g of 1, 3-butanediol was added to dissolve it uniformly, to obtain the composition.

The embodiment also provides jelly which comprises a phase A component, a phase B component and a phase C component; the phase A component comprises 0.1 percent of disodium EDTA (namely disodium ethylene diamine tetraacetate), 0.1 percent of allantoin, 0.08 percent of sodium hyaluronate, 2 percent of glyceryl polyether-26, 1 percent of glycerin, 0.6 percent of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 0.1 percent of carbomer and 80.42 percent of water in percentage by weight; the phase B component comprises 4% of butanediol and 0.6% of p-hydroxyacetophenone; phase C component comprises 1% bamboo charcoal and 10% of the composition provided in this example.

The preparation method of the jelly comprises the following steps: heating and dissolving the B-phase component in a water bath kettle at 80 ℃ in advance until the component is transparent to obtain a B-phase mixture for later use; mixing the phase A components, heating and stirring in a water bath kettle at 80 ℃ until the components are dissolved uniformly, homogenizing for 3 minutes to ensure complete dissolution, and then cooling; cooling to 60 deg.C, adding the mixture of phase B, stirring to dissolve, and cooling; cooling to 45 deg.C, adding C phase component, and stirring; cooling to 37 deg.C, vacuumizing, defoaming, testing color, fragrance, pH, etc., filtering with 200 mesh sieve, and collecting the gel.

Example 2

The embodiment provides a composition for inhibiting acne, which comprises the following raw material components of 18g of tortoise plastron, 18g of glabrous greenbrier rhizome, 12g of radix rehmanniae, 20g of raw licorice, 14g of cape jasmine fruit, 12g of honeysuckle flower and 6g of dahurian angelica root.

The preparation method of the composition comprises the following steps: pulverizing carapax et Plastrum Testudinis, decocting with 180g deionized water for 2 times (each time for 2 hr), mixing decoctions, filtering with 100 mesh gauze, and vacuum concentrating at 70 deg.C in water bath to obtain carapax et Plastrum Testudinis extract; pulverizing the rest raw materials, sieving with 100 mesh sieve, and mixing; then adding into 2L ethanol water solution with volume ratio concentration of 70%, performing microwave extraction under the conditions of frequency of 2000MHz and power of 1000W, extracting at 40 deg.C for 20min, extracting at 75 deg.C under reflux for 30min, extracting for 3 times to obtain mixed extractive solution; dissolving carapax et Plastrum Testudinis extract in the mixed extractive solution, and filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated to 100g under vacuum at 60 ℃ and then 100g of 1, 3-butanediol was added to dissolve it uniformly to obtain the composition.

The embodiment also provides a face cream which comprises a phase a component, a phase b component, a phase c component and a phase d component; the a-phase component comprises 1 percent of argania spinosa kernel oil, 1.2 percent of squalane, 0.4 percent of glyceryl oleate citrate, 0.1 percent of caprylic/capric triglyceride, 6 percent of cyclopentadimethylsiloxane, 1 percent of polydimethylsiloxane and 1 percent of hydrogenated lecithin in percentage by weight; the phase b component comprises 75.25% of deionized water, 0.05% of EDTA disodium, 0.1% of sodium hyaluronate, 2% of glycerol, 0.5% of trehalose, 0.75% of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer (namely acryloyl dimethyl taurate/VP copolymer) and 0.2% of carbomer; the c-phase component comprises 0.15% of aminomethyl propanol, 4% of butanediol, 0.2% of methyl hydroxybenzoate and 0.1% of propyl hydroxybenzoate; the d-phase component comprises 1% of bamboo charcoal and 5% of the composition provided by the embodiment.

The preparation method of the face cream comprises the following steps: heating and dissolving the phase a component in a water bath kettle at 80 ℃ in advance until the phase a component is transparent to obtain a phase a mixture for later use; adding hydroxymethyl and hydroxypropyl in the c-phase component into butanediol, and heating and dissolving in advance until the mixture is transparent to obtain a c-phase mixture for later use; dissolving EDTA disodium, sodium hyaluronate, glycerol, trehalose, ammonium acryloyldimethyl taurate/vinyl pyrrolidone copolymer and carbomer in the phase b components in deionized water, heating and stirring in a water bath kettle at 80 ℃ until the EDTA disodium, the sodium hyaluronate, the glycerol, the trehalose, the ammonium acryloyldimethyl taurate/vinyl pyrrolidone copolymer and the carbomer are dissolved uniformly, and homogenizing for 3 minutes to ensure complete dissolution; stirring at 1500rpm while slowly adding the phase a mixture; after the addition is finished, homogenizing and emulsifying for 8 minutes at the speed of 5000rpm, and then cooling; cooling to 55 deg.C, adding the mixture of phase c and the rest of phase c components, stirring, and cooling; cooling to 45 ℃, adding the phase d component, stirring uniformly, and continuously cooling; cooling to 37 deg.C, vacuumizing, defoaming, inspecting color, fragrance, pH, etc., and filtering with 200 mesh sieve to obtain the final product.

Example 3

The embodiment provides a composition for inhibiting acne, which comprises the following raw material components of 20g of tortoise plastron, 16g of rhizoma smilacis glabrae, 10g of radix rehmanniae, 19g of raw licorice, 14g of fructus gardeniae, 15g of honeysuckle and 6g of radix angelicae.

The preparation method of the composition comprises the following steps: pulverizing carapax et Plastrum Testudinis, decocting with 400g deionized water for 1 hr for 3 times, mixing decoctions, filtering with 100 mesh gauze, and vacuum concentrating at 60 deg.C in water bath to obtain carapax et Plastrum Testudinis extract; pulverizing the rest raw materials, sieving with 100 mesh sieve, and mixing; then adding into 1.6L ethanol water solution with volume ratio concentration of 80%, performing microwave extraction under the conditions of frequency of 3000MHz and power of 600W, extracting at 45 deg.C for 25min, extracting at 70 deg.C under hot reflux for 30min, extracting for 4 times to obtain mixed extractive solution; dissolving carapax et Plastrum Testudinis extract in the mixed extractive solution, and filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated to 100g under vacuum at 55 ℃ and 100g of 1, 3-butanediol was added to dissolve it uniformly to obtain the composition.

Example 4

The embodiment provides a composition, which comprises 16g of tortoise plastron, 20g of glabrous greenbrier rhizome, 14g of radix rehmanniae, 17g of raw licorice, 12g of cape jasmine fruit and 13g of honeysuckle flower.

The preparation method of the composition comprises the following steps: pulverizing carapax et Plastrum Testudinis, decocting with 160g deionized water for 3 times, each time for 1 hr, mixing decoctions, filtering with 100 mesh gauze, and vacuum concentrating at 70 deg.C in water bath to obtain carapax et Plastrum Testudinis extract; pulverizing the rest raw materials, sieving with 100 mesh sieve, and mixing; then adding into 1.4L ethanol water solution with volume ratio concentration of 70%, performing microwave extraction under the conditions of frequency of 3000MHz and power of 800W, extracting at 40 deg.C for 30min, extracting at 80 deg.C under hot reflux for 40min, extracting for 3 times to obtain mixed extractive solution; dissolving carapax et Plastrum Testudinis extract in the mixed extractive solution, and filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated under vacuum at 50 ℃ to 100g, and then 100g of 1, 3-butanediol was added to dissolve it uniformly, to obtain the composition.

The embodiment also provides a face cream which comprises a phase a component, a phase b component, a phase c component and a phase d component; the a-phase component comprises 1 percent of argania spinosa kernel oil, 1.2 percent of squalane, 0.4 percent of glyceryl oleate citrate, 0.1 percent of caprylic/capric triglyceride, 6 percent of cyclopentadimethylsiloxane, 1 percent of polydimethylsiloxane and 1 percent of hydrogenated lecithin in percentage by weight; the phase b component comprises 75.25% of deionized water, 0.05% of EDTA disodium, 0.1% of sodium hyaluronate, 2% of glycerol, 0.5% of trehalose, 0.75% of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and 0.2% of carbomer; the c-phase component comprises 0.15% of aminomethyl propanol, 4% of butanediol, 0.2% of methyl hydroxybenzoate and 0.1% of propyl hydroxybenzoate; the d-phase component comprises 1% of bamboo charcoal and 5% of the composition provided by the embodiment.

The preparation method of the face cream comprises the following steps: heating and dissolving the phase a component in a water bath kettle at 80 ℃ in advance until the phase a component is transparent to obtain a phase a mixture for later use; adding hydroxymethyl and hydroxypropyl in the c-phase component into butanediol, and heating and dissolving in advance until the mixture is transparent to obtain a c-phase mixture for later use; dissolving EDTA disodium, sodium hyaluronate, glycerol, trehalose, an acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and carbomer in the phase b component in deionized water, heating and stirring in a water bath kettle at 80 ℃ until the materials are uniformly dissolved, and homogenizing for 3 minutes to ensure complete dissolution; stirring at 1500rpm while slowly adding the phase a mixture; after the addition is finished, homogenizing and emulsifying at the speed of 5000rpm for 5-10 minutes, and then cooling; cooling to 55 deg.C, adding the mixture of phase c and the rest phase C, stirring, and cooling; cooling to 45 ℃, adding the phase d component, stirring uniformly, and continuously cooling; cooling to 37 deg.C, vacuumizing, defoaming, inspecting color, fragrance, pH, etc., and filtering with 200 mesh sieve to obtain the final product.

Comparative example 1

This comparative example provides an extract comprising 100g of tortoise plastron as a raw material component. The preparation method of the extract comprises the following steps: pulverizing carapax et Plastrum Testudinis, decocting with 160g water for 3 times, each for 1 hr, mixing decoctions, filtering with 100 mesh gauze, and vacuum concentrating at 70 deg.C to 100 g; adding 100g 1, 3-butanediol, and dissolving to obtain the extract.

Comparative example 2

The comparative example provides an extract comprising as raw material components 100g of glabrous greenbrier rhizome.

The preparation method of the extract comprises the following steps: pulverizing Poria, adding 1.4L 70% ethanol water solution, microwave extracting at frequency of 3000MHz and power of 800W for 30min at 40 deg.C, and hot refluxing at 80 deg.C for 40min for 3 times to obtain extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; vacuum concentrating at 50 deg.C to 100g, adding 100g 1, 3-butanediol, and dissolving to obtain the extract.

Comparative example 3

This comparative example provides an extract comprising raw material ingredients of 100g of rehmannia glutinosa libosch.

The preparation method of the extract comprises the following steps: pulverizing radix rehmanniae, adding 1.4L ethanol water solution with volume ratio concentration of 70%, microwave extracting at frequency of 3000MHz and power of 800W for 30min at 40 deg.C, and hot refluxing at 80 deg.C for 40min for 3 times to obtain extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; vacuum concentrating at 50 deg.C to 100g, adding 100g 1, 3-butanediol, and dissolving to obtain the extract.

Comparative example 4

The present comparative example provides an extract comprising 100g of raw licorice as a raw material component.

The preparation method of the extract comprises the following steps: pulverizing Glycyrrhrizae radix, adding 1.4L 70% ethanol water solution, microwave extracting at frequency of 3000MHz and power of 800W for 30min at 40 deg.C, and hot refluxing at 80 deg.C for 40min for 3 times to obtain extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; vacuum concentrating at 50 deg.C to 100g, adding 100g 1, 3-butanediol, and dissolving to obtain the extract.

Comparative example 5

The comparative example provides an extract comprising gardenia jasminoides ellis as a raw material component in an amount of 100 g.

The preparation method of the extract comprises the following steps: pulverizing fructus Gardeniae, adding 1.4L ethanol water solution with volume concentration of 70%, microwave extracting at frequency of 3000MHz and power of 800W for 30min at 40 deg.C, and heat refluxing at 80 deg.C for 40min for 3 times to obtain extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; vacuum concentrating at 50 deg.C to 100g, adding 100g 1, 3-butanediol, and dissolving to obtain the extract.

Comparative example 6

The comparative example provides an extract comprising 100g of honeysuckle as a raw material component.

The preparation method of the extract comprises the following steps: pulverizing flos Lonicerae, adding 1.4L ethanol water solution with volume ratio concentration of 70%, microwave extracting at frequency of 3000MHz and power of 800W for 30min at 40 deg.C, and hot refluxing at 80 deg.C for 40min for 3 times to obtain extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; vacuum concentrating at 50 deg.C to 100g, adding 100g 1, 3-butanediol, and dissolving to obtain the extract.

Comparative example 7

The comparative example provides an extract comprising 100g of radix angelicae as a raw material component.

The preparation method of the extract comprises the following steps: pulverizing radix Angelicae Dahuricae, adding 1.4L 70% ethanol water solution, microwave extracting at 3000MHz and 800W for 30min at 40 deg.C and 40min at 80 deg.C under reflux for 3 times to obtain extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; vacuum concentrating at 50 deg.C to 100g, adding 100g 1, 3-butanediol, and dissolving to obtain the extract.

Comparative example 8

The comparative example provides a composition comprising 16g of tortoise plastron and 20g of glabrous greenbrier rhizome as raw material components.

The preparation method of the composition comprises the following steps: pulverizing carapax et Plastrum Testudinis, decocting in 160g water for 1 hr for 3 times, mixing decoctions, filtering with 100 mesh gauze, and vacuum concentrating at 70 deg.C in water bath to obtain carapax et Plastrum Testudinis extract; pulverizing Poria, sieving with 100 mesh sieve, and mixing; then adding into 1.4L ethanol water solution with volume ratio concentration of 70%, performing microwave extraction under the conditions of frequency of 3000MHz and power of 800W, extracting at 40 deg.C for 30min, extracting at 80 deg.C under hot reflux for 40min, extracting for 3 times to obtain extractive solution; dissolving carapax et Plastrum Testudinis extract in the extractive solution, and filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated under vacuum at 50 ℃ to 100g, and then 100g of 1, 3-butanediol was added to dissolve it uniformly, to obtain the composition.

The comparative example also provides a cream comprising a phase a component, a phase b component, a phase c component, and a phase d component; the a-phase component comprises 1 percent of argania spinosa kernel oil, 1.2 percent of squalane, 0.4 percent of glyceryl oleate citrate, 0.1 percent of caprylic/capric triglyceride, 6 percent of cyclopentadimethylsiloxane, 1 percent of polydimethylsiloxane and 1 percent of hydrogenated lecithin in percentage by weight; the phase b component comprises 75.25% of deionized water, 0.05% of EDTA disodium, 0.1% of sodium hyaluronate, 2% of glycerol, 0.5% of trehalose, 0.75% of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and 0.2% of carbomer; the phase c component comprises 0.15% of aminomethyl propanol, 4% of butanediol, 0.2% of methyl hydroxybenzoate and 0.1% of propyl hydroxybenzoate; the phase d component comprises 1% of bamboo charcoal and 5% of the composition provided by the comparative example.

The preparation method of the face cream comprises the following steps: heating and dissolving the phase a component in a water bath kettle at 80 ℃ in advance until the phase a component is transparent to obtain a phase a mixture for later use; adding hydroxymethyl and hydroxypropyl in the c-phase component into butanediol, and heating and dissolving in advance until the mixture is transparent to obtain a c-phase mixture for later use; dissolving EDTA disodium, sodium hyaluronate, glycerol, trehalose, an acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and carbomer in the phase b component in deionized water, heating and stirring in a water bath kettle at 80 ℃ until the materials are uniformly dissolved, and homogenizing for 3 minutes to ensure complete dissolution; stirring at 1500rpm while slowly adding the phase a mixture; after the addition is finished, homogenizing and emulsifying for 5-10 minutes at the speed of 5000rpm, and then cooling; cooling to 55 deg.C, adding the mixture of phase c and the rest of phase c components, stirring, and cooling; cooling to 45 ℃, adding the phase d component, stirring uniformly, and continuously cooling; cooling to 37 deg.C, vacuumizing, defoaming, inspecting color, fragrance, pH, etc., and filtering with 200 mesh sieve to obtain the final product.

Comparative example 9

The comparative example provides a composition comprising raw material components of 16g of tortoise plastron, 20g of glabrous greenbrier rhizome and 14g of rehmannia root.

The preparation method of the composition comprises the following steps: pulverizing carapax et Plastrum Testudinis, decocting in 160g water for 1 hr for 3 times, mixing decoctions, filtering with 100 mesh gauze, and vacuum concentrating at 70 deg.C in water bath to obtain carapax et Plastrum Testudinis extract; pulverizing the rest raw materials, sieving with 100 mesh sieve, and mixing; then adding into 1.4L ethanol water solution with volume ratio concentration of 70%, performing microwave extraction under the conditions of frequency of 3000MHz and power of 800W, extracting at 40 deg.C for 30min, extracting at 80 deg.C under hot reflux for 40min, extracting for 3 times to obtain mixed extractive solution; dissolving carapax et Plastrum Testudinis extract in the mixed extractive solution, and filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated under vacuum at 50 ℃ to 100g, and then 100g of 1, 3-butanediol was added to dissolve it uniformly, to obtain the composition.

The comparative example also provides a cream comprising a phase a component, a phase b component, a phase c component, and a phase d component; the component of phase a comprises 1 percent of argania spinosa kernel oil, 1.2 percent of squalane, 0.4 percent of glyceryl oleate citrate, 0.1 percent of caprylic/capric triglyceride, 6 percent of cyclopentadecanylsiloxane, 1 percent of polydimethylsiloxane, and 1 percent of hydrogenated lecithin; the phase b component comprises 75.25% of deionized water, 0.05% of EDTA disodium, 0.1% of sodium hyaluronate, 2% of glycerin, 0.5% of trehalose, 0.75% of acryloyl dimethyl ammonium taurate/vinyl pyrrolidone copolymer and 0.2% of carbomer; the c-phase component comprises 0.15% of aminomethyl propanol, 4% of butanediol, 0.2% of methyl hydroxybenzoate and 0.1% of propyl hydroxybenzoate; the phase d component comprises 1% of bamboo charcoal and 5% of the composition provided by the comparative example.

The preparation method of the face cream comprises the following steps: heating and dissolving the phase a component in a water bath kettle at 80 ℃ in advance until the phase a component is transparent to obtain a phase a mixture for later use; adding hydroxymethyl and hydroxypropyl in the c-phase component into butanediol, and heating and dissolving in advance until the mixture is transparent to obtain a c-phase mixture for later use; dissolving EDTA disodium, sodium hyaluronate, glycerol, trehalose, an acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and carbomer in the phase b component in deionized water, heating and stirring in a water bath kettle at 80 ℃ until the materials are uniformly dissolved, and homogenizing for 3 minutes to ensure complete dissolution; stirring at 1500rpm while slowly adding the phase a mixture; after the addition is finished, homogenizing and emulsifying at the speed of 5000rpm for 5-10 minutes, and then cooling; cooling to 55 deg.C, adding the mixture of phase c and the rest of phase c components, stirring, and cooling; cooling to 45 ℃, adding the phase d component, stirring uniformly, and continuously cooling; cooling to 37 deg.C, vacuumizing, defoaming, inspecting color, fragrance, pH, etc., and filtering with 200 mesh sieve to obtain the final product.

Comparative example 10

The comparative example provides a composition, which comprises the raw material components of 6g of tortoise plastron, 20g of glabrous greenbrier rhizome, 14g of rehmannia root, 12g of cape jasmine fruit and 13g of honeysuckle.

The comparative example also provides a cream comprising a phase a component, a phase b component, a phase c component, and a phase d component; the a-phase component comprises 1 percent of argania spinosa kernel oil, 1.2 percent of squalane, 0.4 percent of glyceryl oleate citrate, 0.1 percent of caprylic/capric triglyceride, 6 percent of cyclopentadimethylsiloxane, 1 percent of polydimethylsiloxane and 1 percent of hydrogenated lecithin in percentage by weight; the phase b component comprises 75.25% of deionized water, 0.05% of EDTA disodium, 0.1% of sodium hyaluronate, 2% of glycerol, 0.5% of trehalose, 0.75% of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and 0.2% of carbomer; the c-phase component comprises 0.15% of aminomethyl propanol, 4% of butanediol, 0.2% of methyl hydroxybenzoate and 0.1% of propyl hydroxybenzoate; the phase d component comprises 1% of bamboo charcoal and 5% of the composition provided by the comparative example.

The preparation method of the face cream comprises the following steps: heating and dissolving the phase a component in a water bath kettle at 80 ℃ in advance until the phase a component is transparent to obtain a phase a mixture for later use; adding hydroxymethyl and hydroxypropyl in the phase c component into butanediol, and heating and dissolving in advance until the solution is transparent to obtain a phase c mixture for later use; dissolving EDTA disodium, sodium hyaluronate, glycerol, trehalose, an acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and carbomer in the phase b component in deionized water, heating and stirring in a water bath kettle at 80 ℃ until the materials are uniformly dissolved, and homogenizing for 3 minutes to ensure complete dissolution; stirring at 1500rpm while slowly adding the phase a mixture; after the addition is finished, homogenizing and emulsifying at the speed of 5000rpm for 5-10 minutes, and then cooling; cooling to 55 deg.C, adding the mixture of phase c and the rest of phase c components, stirring, and cooling; cooling to 45 ℃, adding the phase d component, stirring uniformly, and continuously cooling; cooling to 37 deg.C, vacuumizing, defoaming, inspecting color, fragrance, pH, etc., and filtering with 200 mesh sieve to obtain the final product.

Comparative example 11

The comparative example provides a composition comprising raw material components of 16g of tortoise plastron, 20g of glabrous greenbrier rhizome, 14g of radix rehmanniae and 17g of raw licorice.

The preparation method of the face cream comprises the following steps: heating and dissolving the phase a component in a water bath kettle at 80 ℃ in advance until the phase a component is transparent to obtain a phase a mixture for later use; adding hydroxymethyl and hydroxypropyl in the c-phase component into butanediol, and heating and dissolving in advance until the mixture is transparent to obtain a c-phase mixture for later use; dissolving EDTA disodium, sodium hyaluronate, glycerol, trehalose, ammonium acryloyldimethyl taurate/vinyl pyrrolidone copolymer and carbomer in the phase b components in deionized water, heating and stirring in a water bath kettle at 80 ℃ until the EDTA disodium, the sodium hyaluronate, the glycerol, the trehalose, the ammonium acryloyldimethyl taurate/vinyl pyrrolidone copolymer and the carbomer are dissolved uniformly, and homogenizing for 3 minutes to ensure complete dissolution; stirring at 1500rpm while slowly adding the phase a mixture; after the addition is finished, homogenizing and emulsifying at the speed of 5000rpm for 5-10 minutes, and then cooling; cooling to 55 deg.C, adding the mixture of phase c and the rest of phase c components, stirring, and cooling; cooling to 45 ℃, adding the phase d component, stirring uniformly, and continuously cooling; cooling to 37 deg.C, vacuumizing, defoaming, inspecting color, fragrance, pH, etc., and filtering with 200 mesh sieve to obtain the final product.

Comparative example 12

The comparative example provides a composition comprising the raw material components of 16g of tortoise plastron, 20g of glabrous greenbrier rhizome, 14g of rehmannia root and 8g of dahurian angelica root.

Comparative example 13

The comparative example provides a composition comprising raw material components of 17g of raw licorice, 12g of gardenia and 13g of honeysuckle.

The preparation method of the composition comprises the following steps: pulverizing the components, sieving with 100 mesh sieve, and mixing; then adding into 1.4L ethanol water solution with volume ratio concentration of 70%, performing microwave extraction under the conditions of frequency of 3000MHz and power of 800W, extracting at 40 deg.C for 30min, extracting at 80 deg.C under hot reflux for 40min, extracting for 3 times to obtain mixed extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated under vacuum at 50 ℃ to 100g, and then 100g of 1, 3-butanediol was added to dissolve it uniformly, to obtain the composition.

Comparative example 14

The comparative example provides a composition, which comprises the raw material components of 12g of cape jasmine, 13g of honeysuckle and 8g of angelica dahurica.

Comparative example 15

The comparative example provides a composition, which comprises 17g of raw licorice and 8g of angelica dahurica.

Comparative example 16

The comparative example provides a composition, which comprises the raw material components of 17g of raw liquorice, 12g of cape jasmine, 13g of honeysuckle and 8g of angelica dahurica.

The preparation method of the composition comprises the following steps: pulverizing the components, sieving with 100 mesh sieve, and mixing; then adding into 1.4L ethanol water solution with volume ratio concentration of 70%, performing microwave extraction at frequency of 3000MHz and power of 800W for 30min at 40 deg.C, extracting under hot reflux at 80 deg.C for 40min for 3 times to obtain mixed extractive solution; filtering with 100 mesh filter cloth to obtain filtrate; the filtrate was concentrated under vacuum at 50 ℃ to 100g, and then 100g of 1, 3-butanediol was added to dissolve it uniformly, to obtain the composition.

Comparative example 17

The comparative example provides a composition, which comprises 16g of tortoise plastron, 20g of glabrous greenbrier rhizome, 14g of rehmannia root, 17g of raw licorice and 8g of dahurian angelica root.

Comparative example 18

The comparative example provides a composition, which comprises 16g of tortoise plastron, 20g of glabrous greenbrier rhizome, 14g of rehmannia root, 12g of cape jasmine fruit, 13g of honeysuckle flower and 8g of dahurian angelica root.

The comparative example also provides a face cream which comprises a phase a component, a phase b component, a phase c component and a phase d component; the a-phase component comprises 1 percent of argania spinosa kernel oil, 1.2 percent of squalane, 0.4 percent of glyceryl oleate citrate, 0.1 percent of caprylic/capric triglyceride, 6 percent of cyclopentadimethylsiloxane, 1 percent of polydimethylsiloxane and 1 percent of hydrogenated lecithin in percentage by weight; the phase b component comprises 75.25% of deionized water, 0.05% of EDTA disodium, 0.1% of sodium hyaluronate, 2% of glycerol, 0.5% of trehalose, 0.75% of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and 0.2% of carbomer; the c-phase component comprises 0.15% of aminomethyl propanol, 4% of butanediol, 0.2% of methyl hydroxybenzoate and 0.1% of propyl hydroxybenzoate; the phase d component comprises 1% of bamboo charcoal and 5% of the composition provided by the comparative example.

Comparative example 19

The present comparative example provides a gel comprising a phase a component, a phase B component, and a phase C component; the phase A component comprises 0.1 percent of EDTA disodium, 0.1 percent of allantoin, 0.08 percent of sodium hyaluronate, 2 percent of glyceryl polyether-26, 1 percent of glycerin, 0.6 percent of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 0.1 percent of carbomer and 90.42 percent of water in percentage by weight; the phase B component comprises 4% of butanediol and 0.6% of p-hydroxyacetophenone; the phase C component comprises 1% of bamboo charcoal.

The preparation method of the jelly comprises the following steps: heating and dissolving the B-phase component in a water bath kettle at 80 ℃ in advance until the component is transparent to obtain a B-phase mixture for later use; mixing the phase A components, heating and stirring in a water bath kettle at 80 ℃ until the components are dissolved uniformly, homogenizing for 3 minutes to ensure complete dissolution, and then cooling; cooling to 60 deg.C, adding the mixture of phase B, stirring to dissolve, and cooling; cooling to 45 deg.C, adding C phase component, and stirring; cooling to 37 deg.C, vacuumizing, defoaming, inspecting color, fragrance, pH, etc., and filtering with 200 mesh filter to obtain the jelly.

Comparative example 20

The comparative example provides a cream comprising a phase a component, a phase b component, a phase c component, a phase d component; the a-phase component comprises 1 percent of argania spinosa kernel oil, 1.2 percent of squalane, 0.4 percent of glyceryl oleate citrate, 0.1 percent of caprylic/capric triglyceride, 6 percent of cyclopentadimethylsiloxane, 1 percent of polydimethylsiloxane and 1 percent of hydrogenated lecithin in percentage by weight; the phase b component comprises 80.25% of deionized water, 0.05% of EDTA disodium, 0.1% of sodium hyaluronate, 2% of glycerol, 0.5% of trehalose, 0.75% of acryloyl dimethyl taurate/vinyl pyrrolidone copolymer and 0.2% of carbomer; the c-phase component comprises 0.15% of aminomethyl propanol, 4% of butanediol, 0.2% of methyl hydroxybenzoate and 0.1% of propyl hydroxybenzoate; the phase d component comprises 1% of bamboo charcoal.

The compositions provided in examples 1-4 and comparative examples 8-18, and the extracts provided in comparative examples 1-7 correspond to the raw material components and content distribution shown in Table 1.

Table 1 each example and comparative example provide a table of raw material components and content distribution of the composition or extract

Performance testing

1. Evaluation of antibacterial and anti-inflammatory efficacy

The compositions provided by examples 1-4 and comparative examples 8-18 and the extracts provided by comparative examples 1-7 are respectively used as compositions to be tested to carry out antibacterial and anti-inflammatory efficacy evaluation tests, the inhibition rate of propionibacterium acnes and the inhibition rate of outer membrane vesicle release of propionibacterium acnes are used as antibacterial indexes, the inhibition rate of an inflammatory factor IL-8 is used as an anti-inflammatory index, and the specific test method is as follows:

the CCK-8 method is used for measuring the concentration of the composition without cytotoxicity, and the specific implementation method comprises the following steps: THP-1 cells were diluted with RPMI1640 medium containing 10% fetal bovine serum at 2.0X 10⁶Inoculating the mixture with the concentration of each/mL into a 96-well plate, respectively adding the composition to be detected with the concentration of 60 mu g/mL into each 100 mu L-well plate, taking the composition to be detected as an experimental group and taking the composition not to be detected as a blank group, uniformly washing and blowing the mixture, and then placing the mixture at 37 ℃ and 5% CO₂And (5) incubating for 24h in an incubator, and measuring the cell survival rate by using a CCK-8 kit method. The cell viability of the blank group is 100%, and the cell viability is more than 90%, so that the blank group is judged to be non-toxic.

The specific implementation method for detecting the inhibition rate of the composition to be detected on the propionibacterium acnes by using an absorbance method comprises the following steps: adjusting the bacteria concentration to 5.0 × 10 with culture medium⁶CFU/mL, adding the composition to be tested as an experimental group, taking no composition as a blank control, and culturing in a constant temperature incubator at 37 ℃ for 48h under anaerobic condition. The absorbance at 600nm was measured with an ultraviolet spectrophotometer, and the inhibition ratio of Propionibacterium acnes was calculated according to the following formula.

The BCA method is used for determining the release inhibition rate of the outer membrane vesicles of the Propionibacterium acnes, and the specific implementation method comprises the following steps: using a medium containing the composition to be tested at 37 ℃ and 5% CO₂Culturing Propionibacterium acnes under the condition until logarithmic growth phase, taking the composition to be tested as an experimental group, and taking no composition as a blank control. Centrifuging at 4 deg.C and 6000g to obtain supernatant, ultrafiltering with 100KDa fiber membrane, concentrating, filtering with 0.22 μm filter, centrifuging the filtrate at 4 deg.C and 100000g for 3 hr, removing supernatant, washing with PBS for three times, and suspending in PBS to obtain outer membrane vesicle solution; the protein content of the outer membrane vesicles is measured by adopting a BCA kit method, and the outer membrane vesicle release inhibition rate is calculated according to the following formula.

The specific implementation method for measuring the inhibition rate of the inflammatory factor IL-8 comprises the following steps: THP-1 cells were plated at 5X 10⁵Inoculating the mixture with the concentration of one/mL into a 6-pore plate, adding the composition to be detected and propionibacterium acnes, and culturing for 24 hours; measuring the content of IL-8 in cell culture supernatant by using an ELISA kit method; taking the composition to be tested and the propionibacterium acnes as experimental groups, taking the composition not to be tested and the propionibacterium acnes as blank groups, taking the composition not to be tested and the propionibacterium acnes as control groups, and calculating the IL-8 inhibition rate as an anti-inflammatory index according to the following formula.

The cell viability, Propionibacterium acnes inhibition, outer membrane vesicle release inhibition, and IL-8 inhibition for each test composition are shown in Table 2.

TABLE 2 evaluation results of antibacterial and anti-inflammatory efficacy

Note: "-" indicates negative, no effect

According to table 2, all compositions tested were non-toxic; the composition provided by the embodiments 1 to 4 has an inhibition rate of 41 to 52 percent on the reproduction of propionibacterium acnes under the synergistic effect of tortoise plastron, glabrous greenbrier rhizome, rehmannia root, raw liquoric root, gardenia and honeysuckle; the inhibition rate on the outer membrane vesicle release of the propionibacterium acnes reaches 43 to 59 percent, and the inhibition rate on an inflammatory factor IL-8 reaches 37 to 49.6 percent. Therefore, the composition provided by the embodiment of the invention has the effects of inhibiting the propagation of propionibacterium acnes, inhibiting the release of outer membrane vesicles of propionibacterium acnes and inhibiting inflammatory factors.

A certain amount of radix angelicae dahuricae is added on the basis of embodiment 4 of the invention, so that the effects of inhibiting the reproduction of propionibacterium acnes, inhibiting the release of outer membrane vesicles of propionibacterium acnes and inhibiting inflammatory factors are promoted.

Any raw material component is reduced on the basis of the formula of the composition provided by the embodiment of the invention, and the corresponding effects of inhibiting the reproduction of propionibacterium acnes, inhibiting the release of outer membrane vesicles of propionibacterium acnes and inhibiting bacteria and resisting inflammation are reduced.

The extract of tortoise plastron alone has no inhibitory effect on Propionibacterium acnes, outer membrane vesicle release and the anti-inflammatory factor IL-8. Only the glabrous greenbrier rhizome extract has no inhibition effect on the release of the outer membrane vesicles and the anti-inflammatory factor IL-8; only the rehmannia root extract has no inhibition effect on the propionibacterium acnes and the release of outer membrane vesicles; only the gardenia extract has no inhibiting effect on the anti-inflammatory factor IL-8; only the honeysuckle extract has no inhibition effect on the release of outer membrane vesicles; only the angelica dahurica extract has no inhibition effect on the anti-inflammatory factor IL-8; only the extract of tortoise plastron and the extract of smilax glabra rhizome have no inhibitory effect on the anti-inflammatory factor IL-8.

2. Evaluation of oil control effect of human body

The creams provided in the embodiment 2, the embodiment 4 and the comparative examples 8 to 18 are respectively used as the cream to be tested, and the cream provided in the comparative example 20 is used as a blank control to respectively perform the oil control effect test on the human body.

The skin oil content is tested by adopting a skin oil content test probe Sebumeter manufactured by German CK company, and the specific implementation method comprises the following steps: 280 volunteers with age between 25 and 35 and oily skin, half of men and half of women, were selected and randomly divided into 14 groups of 10 women and 10 men each. Volunteers did not use the oil control product one month prior to the test. On the first day of the test, the volunteers sit still for 30min in an environment with the temperature of 22 +/-2 ℃ and the humidity of 45 +/-5% after cleaning the skin, the volunteers keep a relaxed state, the grease data of three positions on the forehead of the volunteers are measured by the tester according to a random table, and the average value is taken. After the test on the first day, volunteers needed to apply the whole face twice in the morning and at night as required, and used the product for 28 consecutive days and returned visits on days 14 and 28.

Taking the grease data obtained by each group test, calculating the grease reduction amount of each person in each group on the 14 th day and the 28 th day, taking the average of the grease reduction amount of each group on the 14 th day as the grease reduction amount of each group on the 14 th day, taking the average of the grease reduction amount of each group on the 28 th day as the grease reduction amount of each group on the 28 th day, and listing the test results of each group in table 3.

TABLE 3 evaluation results of oil control effect

According to table 3, the creams provided in the embodiments 2 and 4 and the comparative examples 8 to 18 of the present invention have different degrees of inhibition effects on skin oil secretion, and the inhibition rate of the creams provided in the embodiments 2 and 4 of the present invention on skin oil secretion can reach more than 30% after 28 days of use, so that the effect of rapidly inhibiting skin oil secretion is achieved.

3. Evaluation of human body's efficacy in inhibiting acne

Evaluation of efficacy against acne tests were carried out with the gels provided in example 1 and comparative example 19, respectively: the acne inhibition efficacy of the traditional Chinese medicine composition of the invention is judged by quantitative analysis of facial features (pigmented spots, texture, pores, wrinkles, red areas and purple) of a VISIA facial image analyzer and professional evaluation of dermatologists. Wherein, the purpurin is porphyrin substance generated in the skin metabolic process, and the porphyrin of the facial skin is mainly derived from the propionibacterium acnes, so the detected purpurin value is in positive correlation with the number of the propionibacterium acnes; the red areas represent the underlying state of the skin, including varicose veins, inflammation and acne of the skin; the value of the red zone is positively correlated with the number of varicose veins, inflammation and acne of the skin; the pore value is mainly used for analyzing the smoothness and the expansion of the sebaceous gland, and the pore value of a common acne patient is larger.

The specific method comprises the following steps: 40 volunteers aged 25-35 and having acne evident on both sides of the face, half of each male and female, were selected, and half-face tests were performed on both sides of the face using gels provided in example 1 and comparative example 19, respectively. Volunteers avoided using other anti-acne products and anti-inflammatory drugs one month prior to the trial. On the 1 st day of the test, after cleaning the skin by using the same face cleaning product, the volunteers sit still for 30min in the environment with the temperature of 22 +/-2 ℃ and the humidity of 45 +/-5%, the faces of the volunteers are shot by using a VISIA facial image analyzer, the pigmented spots, the textures, the pores, the wrinkles, the red areas and the purple quality data of the faces of the volunteers are recorded, the absolute values are used as analysis standards, and dermatologists are asked to count the number of 5 grades of acnes such as white head acnes, black head acnes, papules, cyst nodules and pustules of the volunteers. After the test on the first day, volunteers needed to apply the whole face twice in the morning and at night as required, and used the product for 28 consecutive days and returned visits on days 14 and 28.

The test values were: the data for pigmented spots, wrinkles, lines, pores, purple, and red areas, as well as statistics for whiteheads, blackheads, papules, cystnodules, and pustules are presented in table 4.

TABLE 4 evaluation results of human anti-acne efficacy

According to table 4, after using the gel provided in example 1 for 28 days, various indexes of the face are improved, and the numerical difference of pores, purple and red areas is obvious, which indicates that the oil secretion of volunteers is reduced, the propionibacterium acnes on the face is obviously reduced, and the inflammation of the skin is relieved after using the gel containing the composition of the present invention. Therefore, the composition provided by the invention has a very remarkable inhibiting effect on acne.

According to table 4, after the gel provided in example 1 is used, the number of comedones, papules, cystic nodules and pustules on the face is obviously reduced, which shows that the composition provided in example 1 of the present invention has the effects of resisting bacteria, diminishing inflammation, reducing the secretion of skin oil and improving the influence of acne on the face.

Finally, it should be noted that the above embodiment modes are only used for illustrating the technical solutions of the present invention and are not limited, and although the present invention is described in detail with reference to the above preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The composition is characterized by comprising the following raw material components: tortoise plastron, glabrous greenbrier rhizome, rehmannia root, raw liquoric root, cape jasmine fruit and honeysuckle flower.

2. The composition of claim 1, wherein the tortoise plastron is a tortoise plastron.

3. The composition of claim 1, further comprising the following raw material components: radix Angelicae Dahuricae.

4. The composition according to claim 3, which comprises the following raw material components in parts by weight: 1-20 parts of tortoise plastron, 1-25 parts of glabrous greenbrier rhizome, 1-20 parts of radix rehmanniae, 1-20 parts of raw liquorice, 1-15 parts of cape jasmine fruit, 1-15 parts of honeysuckle and 1-10 parts of dahurian angelica root;

preferably, the composition comprises the following raw material components in parts by weight: 15-20 parts of tortoise plastron, 15-20 parts of glabrous greenbrier rhizome, 8-15 parts of rehmannia root, 15-20 parts of raw liquoric root, 10-15 parts of cape jasmine fruit, 10-15 parts of honeysuckle flower and 5-9 parts of dahurian angelica root.

5. A process for the preparation of a composition according to any one of claims 1 to 4, characterized in that it comprises the following steps:

obtaining extracts of the raw material components;

mixing the extracts of the raw material components to obtain the composition.

6. The method according to claim 5, wherein the extracts from the raw material components are:

pulverizing carapax et Plastrum Testudinis, decocting, filtering, and concentrating to obtain carapax et Plastrum Testudinis extract;

pulverizing the rest raw materials, extracting with solvent, and refluxing to obtain mixed extractive solution.

7. The method according to claim 6, wherein the solvent is at least one of water, methanol, and ethanol; preferably, the extraction is microwave extraction, the frequency of the extraction is 2000-3000MHz, the power is 600-1000W, the temperature is 40-60 ℃, and the time is 20-60 min.

8. Use of a composition according to any one of claims 1 to 4 for the preparation of a cosmetic product.

9. A cosmetic comprising the composition according to any one of claims 1 to 4.

10. The cosmetic according to claim 9, wherein the composition is added in an amount ranging from 0.1% to 20% by weight relative to the total weight of the cosmetic;

preferably, the cosmetic is a gel, a cream, a mask, a lotion, an essence or a lotion.