CN109288931A - A kind of composition for treating infant eczema, preparation and preparation method and application - Google Patents

Abstract

The invention discloses a kind of composition for treating infant eczema, preparation and preparation method and application.The preparation includes active constituent D, component A, component B, component C；By weight, the active constituent D includes following component: 1 part of overlay film saccharomycetes to make fermentation Product filtrate, 0.45 part of plant extracts；The component A includes following component: 0.06 part of EDTA- disodium, 3 parts of glycerol, 2 parts of butanediol, 0.03 part of xanthan gum, 0.02 part of low-acyl gellan gum, 0.02 part of high acyl gellan gum, 90 parts of water.The application is combined using overlay film saccharomycetes to make fermentation product and the traditional Chinese medicine ingredients with heat-clearing and damp-drying drug, the eczema position of skin can be uniformly applied to, to fully absorb drug, make local vasoconstriction, hyperemia is reduced, and inflammatory reaction mitigates, thus the symptom for the treatment of or alleviation eczema.

Description

A kind of composition for treating infant eczema, preparation and preparation method and application

Technical field

The present invention relates to pharmaceutical technology fields, more particularly to a kind of composition for treating infant eczema, preparation and system Preparation Method and application.

Background technique

Infant eczema is dermatology common disease and frequently-occurring disease, and motherland's medicine is referred to as " infantile eczema " or " infantile eczma ", in recent years To think that this disease is the infantilism of atopic dermatitis.It is that one kind as caused by a variety of inside and outside factors has obvious exudation tendency Scytitis.It is showed according to skin lesion, eczema can be divided into acute, subacute, chronic three kinds.Less serious case's local skin erythema, mound Rash has secretion exudation；For severe one with rotten to the corn itch for main recurrent exerbation, obstinate seriously affects the health of infant And normal development.

At present, clinical multi-purpose corticosteroid hormone and its compound preparation added with antibacterials treat infant eczema, But long-time service can produce dependence and various adverse reactions, such as atrophoderma, telangiectasis, pigmentation.And Since infant's skin is thin tender, body surface area is relatively large, therefore it is more that percutaneous drug absorption amount is more adult, if continuous external application skin Matter steroid hormone, overlong time, area is excessive, then more adult to be easier to generate hypothalamic-pituitary-adrenal by systemic Absorption The inhibition of axis, even if weak effect corticosteroid hormone, prolonged application also may occur in which growth inhibition phenomena.

Traditional Chinese medicine has thousands of years medical practice experiences in China, with its therapeutic effect outstanding and side effect it is low and by China or even attention all over the world.With the raising year by year of infant eczema disease incidence, the therapy approach of Chinese medicine is found, it can be with Avoid the side effect of infant prolonged application hormone, antibiotic and antihistamine.Based on the above reasons and according to classical ancient prescription, in conjunction with Modern pharmacology research and clinical practice result, we select have restrain, dry, is anti-inflammatory, the medium-height grass of antiallergy, bacteriostasis Medicine compound combines modern some anti-inflammatory active components, constitutes the combination that can treat infant's dermatitis and eczema.

Summary of the invention

In order to solve the above-mentioned technical problem, one aspect of the present invention provides a kind of composition for treating infant eczema, packet Active constituent D is included, by weight, the active constituent D includes following component: overlay film saccharomycetes to make fermentation Product filtrate 0.5-3 Part, 0.1-1 parts of plant extracts.

As a kind of perferred technical scheme, a kind of composition for treating infant eczema includes active constituent D, is pressed Parts by weight meter, the active constituent D include following component: 1 part of overlay film saccharomycetes to make fermentation Product filtrate, plant extracts 0.45 Part.

As a kind of perferred technical scheme, the plant extracts be selected from Herba Violae extract, dandelion extract, Herba hedyotis diffusae extract, mint extract, phellodendron extract, coptis extract, Chinese rhubarb extract, Baical Skullcap root P.E, cnidium monnieri Seed extract, nutgall extractive, atractylodes chinensis, polygonum cuspidate root extract, Herba Schizonepetae extract, Armstrong extract, shaggy-fruited dittany root skin One of extract, perilla leaf extract, Radix Sangusorbae extract, Honegsukle flower P.E, houttuynia extract, shrubby sophora extract or It is a variety of.

As a kind of perferred technical scheme, the plant extracts is Herba Violae extract, common cnidium fruit P.E, Huang Cypress extract, coptis extract, shaggy-fruited dittany root bark extract, polygonum cuspidate root extract, Armstrong extract, atractylodes chinensis, kuh-seng mention The combination of object is taken, corresponding weight part ratio is (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5)。

As a kind of perferred technical scheme, the plant extracts is houttuynia extract, Honegsukle flower P.E, five times Seed extract, shrubby sophora extract, mint extract, phellodendron extract, perilla leaf extract, Radix Sangusorbae extract, oldenlandia diffusa The combination of extract, corresponding weight part ratio are (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5)。

As a kind of perferred technical scheme, the plant extracts is Herba Violae extract, Chinese rhubarb extract, the coptis Extract, phellodendron extract, shrubby sophora extract, dandelion extract, Herba Schizonepetae extract, Armstrong extract, atractylodes chinensis Combination, corresponding weight part ratio are (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5).

Second aspect of the present invention additionally provides a kind of preparation, by including a kind of composition system for treating infant eczema Standby to obtain, dosage form is pellet, emulsus, any one in paste.

As a kind of perferred technical scheme, the preparation further includes component A, component B, component C；Wherein, by weight Meter, the component A includes following component: 0.1-0.1 parts of EDTA- disodium, 1-8 parts of glycerol, 1-5 parts of butanediol, xanthan gum 0.01- 0.05 part, 0.01-0.05 parts of low-acyl gellan gum, 0.01-0.05 parts of high acyl gellan gum, 70-110 parts of water；The component B packet Include following component: 1-3 parts of Glyceryl stearate citrate, 0.5-1.5 parts of stearyl alcohol glycyrrhetin acid esters, 0.1-1 parts of avocado oil, 0.1-1 parts of dipotassium glycyrrhizinate, 0.1-0.5 parts of methyl hydroxybenzoate, 0.1-0.5 parts of ethyl hydroxy benzoate；The component C includes following component: 0.1-0.5 parts of evening primrose oil, 0.1-0.5 parts of olibanum essential oil, 0.1-0.5 parts of bisabolol, aurantiamarin methyl chalcone 0.1-0.5 Part, 0.01-0.1 parts of tetrahydro piperine.

Third aspect present invention additionally provides a kind of preparation method of preparation, includes the following steps:

(1) the EDTA- disodium in component A is added to the water, is stirred to dissolve uniformly, then by the Huang in component A Virgin rubber, which is added in glycerol propylene glycol, stirs into uniform slurry, then slurry is added to the water, and be heated to 70-80 ℃；

(2) B component is mixed and heated to 80-90 DEG C；

(3) component A is then stirred on one side, B component is added in A on one side, homogeneous 5 minutes, keep the temperature 20 minutes, then drop Temperature；

(4) after near 35-50 DEG C of temperature, component C is added and stirs evenly；

(5) after near 25-40 DEG C of temperature, active constituent D is added and stirs evenly；

Fourth aspect present invention additionally provides a kind of application of composition for treating infant eczema, the combination Object is for treating infant eczema.

Compared with prior art, the invention has the benefit that a kind of composition for treating infant eczema in the application, Preparation and preparation method and application, cordate houttuynia therein, viola mandshurica is clearing heat and detoxicating, is monarch drug in a prescription；Frutus cnidii, dandelion, Cortex Phellodendri, Kuh-seng, cortex dictamni, polygonum cuspidate, garden burnet and oldenlandia diffusa energy heat-clearing and damp-drying drug, timid wind analgesic, is altogether ministerial drug；Perilla leaf can dispelling wind stop Itch, peppermint and schizonepeta energy inducing diaphoresis relieve heat, promoting eruption, rhizoma atractylodis can eliminate dampness with aromatics relieve stagnation, ward off dirty eliminating dampness, to be altogether adjutant；Chinese gall, function Based on astringency inducing, the through sick position of all medicines is guided, to make medicine.It is antipruritic to play clearing heat and detoxicating, clearing damp convergence altogether for all medicines share Function.The application is combined using overlay film saccharomycetes to make fermentation product and the traditional Chinese medicine ingredients with heat-clearing and damp-drying drug, can equably be acted on In the eczema position of skin, to fully absorb drug, make local vasoconstriction, hyperemia is reduced, and inflammatory reaction mitigates, to control Treat or alleviate the symptom of eczema.

The application has the function of heat-clearing, dehumidifying, dispelling wind and arresting itching.It and is pure Chinese medicinal preparation, the adverse reaction of no hormone.Baby Child's dermatitis patients age of onset is smaller, and skin is tender, is not suitable for using the strong externally applied drug of irritation, and the prescription action temperature It is suitable for being treated to this kind of patient with, no obvious irritation.

Detailed description of the invention

Fig. 1 is to use the effect picture before embodiment 1；

Fig. 2 is to use the effect picture after embodiment 1；

Fig. 3 is to use the effect picture before embodiment 2；

Fig. 4 is to use the effect picture after embodiment 2；

Fig. 5 is the anti-inflammatory experimental data figure of the LPS inducing macrophage of embodiment 1.

Specific embodiment

The detailed description for preferred implementation method of the invention below of participating in the election of and including embodiment this hair can be more easily to understand Bright content.Unless otherwise defined, all technologies used herein and scientific term have common with fields of the present invention The normally understood identical meaning of technical staff.When there is a conflict, the definition in this specification shall prevail.

For the purpose of following detailed description, it should be understood that the present invention can be used various substitutions variation and step it is suitable Sequence, unless specifically stated on the contrary.In addition, being indicated in the case where in addition in any operational instances or otherwise pointing out Such as all numbers of the amount of ingredient used in description and claims should be understood in all cases by term " about " it modifies.Therefore, unless indicated to the contrary, the numerical parameter otherwise illustrated in the following description and appended dependent claims is root The approximation changed according to the expected performance of the invention to be obtained.It is at least not intended to for the applicable of doctrine of equivalents being limited in In the scope of the claims, each numerical parameter should at least be given up according to the number of the effective digital of report and by the way that application is common Enter technology to explain.

Although illustrating that broad range of numberical range and parameter of the invention are approximations, listed in specific example Numerical value is reported as accurately as possible.However, any numerical value inherently includes the standard deviation by finding in its each self-test measurement The certain errors necessarily led to.

When a numberical range disclosed herein, above range is considered as continuously, and the minimum value including the range and most Big value and each value between this minimum value and maximum value.Further, when range refers to integer, including the model Each integer between minimum value and maximum value enclosed.In addition, when providing multiple range Expressive Features or characteristic, Ke Yihe And the range.In other words, unless otherwise specified, otherwise all ranges disclosed herein are understood to include and are wherein included into Any and all subrange.For example, should be regarded as including between minimum value 1 and maximum value 10 from the specified range of " 1 to 10 " Any and all subrange.The Exemplary range of range 1 to 10 include but is not limited to 1 to 6.1,3.5 to 7.8,5.5 to 10 etc..

Viola mandshurica:(scientific name: Viola philippica) is the herb of violaceae plant viola mandshurica.Bitter, it is pungent, It is cold.Converge to heart and liver channels.With clearing heat and detoxicating, cool blood detumescence, the effect of clearing heat and promoting diuresis.The complex chemical composition of viola mandshurica, mainly Containing plurality of active ingredients such as organic acid, carbohydrate, Coumarins, flavones and its glycosides, and be enriched with Cu, Fe, Mn, Zn, Ca, P, The microelements such as Si, microelement have certain influence to the activity of internal a variety of enzymes, and function is immunized in the synthesis to nucleic acid protein Can, cell proliferation plays the role of direct or indirect, and it can promote the reparation of epithelial cell, increase cell division, T cell number Increase, increased activity, to play regulation and control effect to the metabolism of living organism and immune function by enzyme system.

Dandelion:(Classification system: Taraxacum mongolicum Hand.-Mazz.) property is bitter, sweet, cold.Return liver, stomach Through.There is the benefits of clearing heat and detoxicating, dispelling wind and heat from the body." materia medica " is recorded: its property of dandelion is refrigerant, controls all and treats sore, large carbuncle, redness The all diseases of heat toxin, " a supplement to augmented materia medica " say its Detoxicating food-poisoning, dissipate stagnant gas, change heat toxin, disappear and dislike swollen, tuberculosis, treat and swell.Modern pharmacology is general All over think dandelion have broad-spectrum antibacterial effect, to gold-coloured staphylococci, staphylococcus epidermis, hemolytic streptococcus, catarrh ball Bacterium has significant inhibiting effect, part antibiotic can be replaced for clinic.

Oldenlandia diffusa:It is (scientific name: Hedyotis diffusa) property slight bitter, sweet, cold.Return stomach, large intestine, small intestinl channel.Have clear The benefits of thermal detoxification, dampness removing is treating stranguria.Total flavones of oldenlandia diffusa willd can enhance body specificity and non-specific immune function, extract In polyphenol, flavones, hydroxy-anthraquione there is antioxidation, cupreol has an anti-inflammatory effect, and ursolic acid has stable cooling, anti- The effects of bacterium, anti-inflammatory.

Peppermint:(scientific name: Mentha haplocalyx Briq.) is pungent, cool.Channel tropism return lung, Liver Channel.Materia Medica of Decoction note It carries: the big yin of hand, JUE YIN meridian medicine." detailed outline " is recorded: start with less, is lunar, yin of fainting enough." book on Chinese herbal medicine is newly organized " is recorded: entering lung and envelope two Also it can be entered liver, gallbladder.Dirty, removing toxic substances is warded off in dispelling wind, heat dissipation.

Cortex Phellodendri:(scientific name: Platycladus orientalis (Linn.) Franco) is bitter, trembles with fear.Channel tropism returns kidney, bladder meridian. Heat-clearing and damp-drying drug, purging intense heat, except steaming, detoxification sore treatment.Shennong's Herbal is recorded: heat, jaundice, perianal abscess are tied in main the five internal organs stomach；Stopping leak dysentery, woman Whitish metrorrhagia, acute vulva ulcer." Bie Lu " is recorded: frightened gas is treated between skin, red of skin heat, and the red pain of mesh heat, aphtha.Supplement to the Herbal Record: main herpes simplex blister rises, worm sore, dysentery, and lower blood kills moth；Decoction, master quench one's thirst.

The coptis:(scientific name: Coptis chinensis Franch.) bitter is trembled with fear, and can rise can drop, and yin is nontoxic.Enter the heart with Collaterals of the uterus.Most purging intense heat can also enter liver.Compendium of Material Medica is recorded: the coptis big bitter Great Cold, with fall fire eliminating dampness, middle disease is i.e. when only.Mainly Chemical component has 2 class of alkaloid and lignanoid, additionally includes phenolic acid, volatile oil, flavonoids, cumarin, terpene, steroid Body, polysaccharide etc..

Rheum officinale:For polygonum rheum palmatum Rheum palmatum L., Rheum tanguticum Maxim Rheum tanguticum The drying root and rhizome of Maxim.ex Balf. or Rheum officinale Rheum officinale Baill..Autumn end cauline leaf it is withered or Excavation before secondary spring is germinateed, removes radicula, scrapes off crust, valve cutting or section, and rope wears bunchiness drying or convection drying.Nature and flavor: it is bitter, it trembles with fear. Channel tropism: returns spleen, stomach, large intestine, liver, pericardium channel." Bie Lu " is recorded: flat stomach, lower gas, eliminating phlegm is real, and heat, trusted subordinate's turgor, female are tied between intestines The cold blood of son closes swollen, the pain of lower abdomen, and all old blood stays knot." haigoushen " is recorded: main fever and chills help digestion, and refine the five internal organs, logical woman is through waiting, Li Shui It is swollen, break that phlegm is real, and cold and hot accumulation, place food, sharp intestine and small intestine, patch heat toxin is swollen, disease when main children's fever and chills, dysphoria with smothery sensation loses purulence, broken to stay blood." guiding principle Mesh " it records: it is also white to cure mainly diarrhea, inner colic, dribbling urination, real heat constipation, hectic fever delirious speech, jaundice, all fire sores.

Radix scutellariae:(Classification system: Scutellaria baicalensis Georgi) property is bitter, cold.It is return lung, gallbladder, spleen, big Intestines, small intestinl channel.There are the benefits of heat-clearing and damp-drying drug, purging fire for removing toxin, dampness-heat in upper jiao in You Shanqing.To staphylococcus aureus, hemolytic chain The gram-negative bacterias such as the gram positive bacterias such as coccus and Escherichia coli, Pseudomonas aeruginosa have different degrees of inhibiting effect, wherein yellow A kind of reed mentioned in ancient books glycosides etc. has inhibiting effect to active chronic inflammation, and can reduce capillary permeability, reduces the release of Anaphylactic mediator, has Significant anti-allergic effects.

Frutus cnidii:(scientific name: Cnidium monnieri (L.) Cuss.) alias cicely, is umbelliferae cnidium monnieri Dry mature fruit.It is warm-natured, bitter.Eliminating dampness, wind-dispelling, desinsection, warming kidney and enhancing body.Fruit contains volatile oil 1.3%, and main component is Firpene, amphene, bornival, isoborneol.Contain osthole, cnidimine, isopimpinellin, in bergamot again Ester, columbianetin and its angelate, acetic acid esters and isovalerate, cnidiadin, isobutyl acyloxy o-acetylcolumbianetin. Root contains cnidimine, isopimpinellin, alloimperatorin, xanthotoxol, ammidin.With antimycotic, antiviral, drive The effects of roundworm, anti-trichomonal.

Chinese gall:(scientific name: Rhus chinensis Mill.) is sour, puckery, trembles with fear.Channel tropism return lung, large intestine, kidney channel.Astringing lung-QI drop Fire, relieving diarrhea with astringents, arrest sweating hemostasis, hygroscopic sore." book on Chinese herbal medicine is looked for the truth " is recorded: Chinese gall, and eucalyptus book had both carried sour and puckery, and gas is cold to be held back Lung channel superficial heat is resolving sputum excreting dampness, the agent of fall fire inducing astrigency；Speech is main in rheumatism again, and all wind tinea are itched scabies, and hot eyes ophthalmodynia can also have with it Effect.In vitro test is to staphylococcus aureus, streptococcus, pneumococcus and typhoid fever, paratyphoid, dysentery, anthrax, diphtheria, green Purulence bacillus etc. has apparent antibacterial or bactericidal effect.

Rhizoma atractylodis:(scientific name: Atractylodes Lancea (Thunb.) DC.) is compositae plant Atractylis lancea, Atractylis chinensis, pass The rhizome of rhizoma atractylodis.It is pungent, bitter, temperature.Channel tropism returns spleen, stomach, Liver Channel.It is drying damp and strengthening spleen, expelling wind and clearing away cold, improving eyesight.Rhizoma atractylodis rhizome contains volatile oil About 5~9%.The main component of oil is atractylol, hinesol, sagittol etc.." detailed outline " is recorded: it is sweet and pungent strong, it is warm-natured and dry, Negative middle-jiao yang, function of the spleen and stomach, can rise can drop.Enter that foot too yin, yang is bright, and hand is lunar, the sun warp.

Polygonum cuspidate:(scientific name: Polygonumcuspidatum Sieb.et Zucc.) slight bitter, it is slightly cold.Return liver, gallbladder, lung channel. Root and rhizome, which contain to dissociate, fears quinone and anthraquinone glycoside.Predominantly rheum emodin, Physcion and Chrysophanol and anthracene glucoside A, anthracene glucoside B.Separately Containing tannin and several polysaccharide.There are antibacterial, antivirus action.

Schizonepeta:For the dry aerial parts of Lamiaceae plant schizonepeta Schizonepeta tenuifolia Briq..It is pungent, Tepor." detailed outline " is recorded: schizonepeta, enters meridian of Foot-JUE YIN gas point, and function is longer than dispelling wind evil, and scattered blood stasis breaks knot gas, and disappear sore.Lid is fainted Yin is wind wood, main blood and phase fire posts it.Therefore wind is sick, blood disease, sore disease are key medicine.Shennong's Herbal is recorded: main fever and chills, the plague, scrofula Raw sore, breaks knot gather qi together, and lower hemostasis removes arthritis with fixed pain caused by dampness.Channel tropism return lung, Liver Channel.Inducing diaphoresis relieve heat, promoting eruption.It is main in oil containing volatile oil 1.8% Ingredient is D-menthone, racemization book lotus ketone, a small amount of d-limonen.Make with antipyretic-antalgic, resisting pathogenic microbes, hemostasis etc. With.

Armstrong:For the dry aerial parts of polygonaceae plant Armstrong Polygonum aviculare L..When summer leafiness Harvesting removes root and impurity, dries.Nature and flavor: it is bitter, it is slightly cold.Return bladder meridian.Inducing diuresis for treating strangurtia, desinsection are antipruritic.Armstrong Han pivot Avicularin, Quercitrin, d- cachou extract, gallic acid, caffeic acid, oxalic acid, silicic acid, chlorogenic acid, p- coumaric acid, cement, glucose, fructose and The chemical components such as sucrose.Have the effects that diuresis, decompression, antibacterial.

Shaggy-fruited dittany root skin:For the dry root skin of rutaceae shaggy-fruited dittany Dictamnus dasycarpus Turcz..Spring, autumn Two seasons excavated root, removed silt and rough bark, stripped root skin, dry.Nature and flavor: it is bitter, it trembles with fear.Returns spleen, stomach, bladder meridian.Root contains shaggy-fruited dittany It is alkali, dictamnolactone, sitosterol, obacunonic acid, trigonelline, choline, Ash skin ketone, campesterol, skimmianine, γ-fagarine, white The chemical components such as distinct alkali.Shennong's Herbal is recorded: main intermittent headache, jaundice, cough is inverse, leaching drip, swells and ache in woman's yin, the dead flesh of arthritis with fixed pain caused by dampness can not It bends and stretches, start-stop, row step." Bie Lu " is recorded: it is uneasy to treat four limbs, when row abdomen in it is too hot, drink water, be intended to away, calling out, pediatric epilepsy scared, woman People's postpartum remaining pain." haigoushen " is recorded: controlling all heat toxin wind, foul wind, wind sore, scabies are red rotten, and eyebrow hair takes off crisp, musculus cutaneus urgency, high fever Aversion to cold；Principal solution heat Huang, wine Huang, acute febrile, paddy Huang, labor Huang etc..

Perilla leaf:For the dried leaf of Lamiaceae plant purple perilla Perilla frutescens (L.) Britt.Summer branches and leaves cyclopentadienyl Flower harvesting, removes impurity, dries.Nature and flavor: acrid in flavour and warm in nature.Return lung, the spleen channel.Relieving exterior syndrome and dispelling cold, relieving QI stagnancy in the stomach.Wrinkle purple perilla herb, which contains, to be waved Hair oil about 0.5%, includes perillaldehyde about 55%, and left-handed limonene 20~30% and australene are a small amount of.Also containing arginine, cumfrey, Cyanidin 3- (6- is to coumaric acyl-β-D-Glucose glucoside) 5- β-D-Glucose glucoside.Also, containing isoegomaketone in the volatile oil of leaf Deng.Sharp purple perilla herb contains volatile oil, includes isopentyl -3- furyl ketone, perillaldehyde, α-and nopinene, (R)-4-isopropenyl-1-methyl-1-cyclohexene, l- virtue camphor tree Alcohol, amphene, menthol, menthones, perilla alcohol, dihydroperilla alcohol, Eugenol.Has the effects that antipyretic, antibacterial.

Garden burnet:For the dry root of rosaceous plant garden burnet Sanguisorba officinalis L..Season will germinate when or It is excavated after autumn plant is withered, removes fibrous root, cleaning, drying, or take advantage of fresh slices, it is dry.Nature and flavor: it is bitter, sour, puckery, it is slightly cold.Cool blood Hemostasis, removing toxic substances sore.Root contains tannin about 17%, triterpenoid saponin 2.5~4.0%.The saponin isolated has: ziyu glycoside I, hydrolysis Pomolic acid, arabinose and glucose are generated afterwards；Ziyu glycoside II generates pomolic acid and arabinose after hydrolysis；Ground persimmon Saponin B, Preliminary Identification are the triterpenoid saponins of glucuronic acid.Glucoside of the cauline leaf containing Quercetin and Kaempferol, the triterpenes such as ursolic acid Substance.Leaf contains vitamin C.Flower is containing Chrysanthemin, the double glucoside pressure effects of corn flower.Shennong's Herbal is recorded: housewife Ren Ru Chi pain, seven kinds of impairments, Vaginal discharge diseases, analgesic, except meat is disliked, hidroschesis treats metal-inflicted wound." Bie Lu " is recorded: only purulence blood, all fistulas, malignant sore, disappear wine, except quenching one's thirst, mends exhausted Wound, postpartum internal plug can make metal-inflicted wound cream." haigoushen " is recorded: hemostasis dysentery loses purulence.

Honeysuckle:(scientific name: Lonicera japonica Thunb.) property is sweet, cold.Return lung, village, stomach meridian.There is heat-clearing solution The benefits of poison, dispersing swelling and dissipating binds." this is through meeting source " road its " the timid purulence of removing toxic substances, rush down in there is benefit, ulcer to burst after panacea ".Honeysuckle is to golden yellow Color staphylococcus, beta streptococcus have obvious inhibiting effect, there is certain inhibiting effect to acute and chronic, granulomatous inflammation, DPPH free radical and peroxynitrite, and the generation of inhibitory activity oxygen and Zhe Ji free radical can be significantly removed, is had good The anti-seedling of wide spectrum well, anti-inflammatory and antioxidation.

Cordate houttuynia:The dry aerial parts of saururaceae plant houttuynia cordata Houttuynia cordata Thunb..Summer stem Leafiness flower spike is tapped when more, is removed impurity, is dried.Nature and flavor: it is pungent, it is slightly cold.Channel tropism: distributed in lung channel.It is clearing heat and detoxicating, the carbuncle that disappears apocenosis, Inducing diuresis for treating strangurtia.Herb contains volatile oil 0.0049%, decanoy acetaldehyde containing antimicrobial component, methyl n nonyl ketone, laurene, laurel in oil Aldehyde, capraldehyde, capric acid.Still chloride containing potassium, potassium sulfate, cordarine.Flower spike, fruit ear contain Isoquercitrin, and leaf contains quercitrin.Also it has been reported that Flower, leaf, the flavonoids in fruit are identical, all contain Quercetin, quercitrin, Isoquercitrin, reynoutrin, hyperin.Rhizome volatile oil Also contain decanoy acetaldehyde.With antibacterial, antiviral, diuresis, anastalsis.

Kuh-seng:For the dry root of leguminous plant kuh-seng Sophora flavescens Ait..It spring, the excavation of two season of autumn, removes Root head and small supporting root, cleaning, drying, or fresh slices are taken advantage of, it is dry.Nature and flavor: it is bitter, it trembles with fear.Channel tropism: return heart, liver, stomach, large intestine, bladder Through.Root contains a variety of alkaloids: d-Matrine, d-Oxymatrine, sophoranl l-Anagyrine, l- methylcytisine, l- wear leaf Counterfeit indigo alkali and sophocarpine.Also containing flavonoids: Xanthohumol, isoxanthohumol, 3,4 ', 5-, tri- hydroxyl -7- methoxy -8- isoamylene radical chromocor, 8-prenylkaempferol etc..Stem, the leaf glycoside of -7- containing luteolin." book on Chinese herbal medicine is newly organized " is recorded: entering the heart, lung, kidney, large intestine Through.Shennong's Herbal is recorded: main stagnation of vital energy in the chest and abdomen, a lump in the abdomen causing distension and pain, jaundice, and drowning has the heeltap, relieves oedema or abdominal distension through diuresis or purgation, and removes carbuncle swells, bowl spares, and improving eyesight stops tear.

For the theory of Chinese medicine: infant's dermatitis is considered native endowment deficiency, loses heat fetus or the day after tomorrow by parent Feeding desorder causes spleen deficiency wet from interior life, and the strongly fragrant longization heat of dyspepsia causes the body of deficiency of the spleen and heat of the stomach, exogenous disease wind, wet, heat evil, Yu Yupi It falls ill between skin space between skin and muscles.

A kind of composition for treating infant eczema in the application, the cordate houttuynia in preparation, viola mandshurica is clearing heat and detoxicating, is Monarch drug in a prescription；Frutus cnidii, dandelion, Cortex Phellodendri, kuh-seng, cortex dictamni, polygonum cuspidate, garden burnet and oldenlandia diffusa energy heat-clearing and damp-drying drug, timid wind analgesic, It is altogether ministerial drug；Perilla leaf can dispelling wind it is antipruritic, peppermint and schizonepeta energy inducing diaphoresis relieve heat, promoting eruption, rhizoma atractylodis can eliminate dampness with aromatics relieve stagnation, ward off it is dirty dry It is wet, to be altogether adjutant；Chinese gall, function guide the through sick position of all medicines based on astringency inducing, to make medicine.All medicines share, altogether It plays clearing heat and detoxicating, clearing damp and restrains antipruritic function.The application has the function of heat-clearing, dehumidifying, dispelling wind and arresting itching.It and is pure Chinese medicine system Agent, the adverse reaction of no hormone.Infant's dermatitis morbidity's age is smaller, and skin is tender, is not suitable for strong using irritation Externally applied drug, and the prescription action temperature and, no obvious irritation, be suitable for this kind of patient is treated.

Infant eczema by lost in tire heat, evil legacy or feeding desorder, the dysfunction of the spleen in transport, it is interior accumulate it is damp and hot, outside by the institutes such as ailment said due to cold or exposure It causing, western medicine is still based on corticosteroid hormone external preparation, though effective in cure, easy recurrence, and skin lesion disperses, face Often larger, the Reusability glucocorticoids ointment of product, is easy to cause thinning of skin, atrophy and other side effects to occur, And it is be easy to cause hormone dependant, and be not suitable for infant and use, it is especially facial.Chinese medicine external treatment can be obviously improved acute and sub- The acute atopic eczema state of an illness, children's body surface area is relatively large, and skin is thin, good absorbing, while Chinese medicine external, conveniently makes With.

Cordate houttuynia acrid flavour in the application, it is cold, it is distributed in lung channel.It can sore be treated in clearing heat and detoxicating, detumescence, promoting urination and removing dampness, heat-clearing stop Monarch drug in a prescription is thought in dysentery, stomach strengthening and digestion promoting, reuse；Honeysuckle is clearing heat and detoxicating, dispelling wind and heat from the body, Cortex Phellodendri heat-clearing and damp-drying drug, and purging fire for removing toxin can be used for Sore swollen toxin, eczema itch, Asian puccoon is cold in nature, bitter, has effects that cooling and activating blood, more than removing toxic substances promoting eruption three taste medicines are all ministerial drug； Garden burnet, cold in nature, bitter acid are nontoxic；Return liver, lung, kidney and large intestine channel.There are cooling blood and hemostasis, healing up sore and subduing swelling and other effects, perilla leaf external application Can dispelling wind it is antipruritic, the two and be adjutant, Schisandra chinensis, function guides the through sick position of all medicines based on astringency inducing, to make medicine. All medicines share plays clearing heat and detoxicating, clearing damp altogether and restrains antipruritic function.

Cordate houttuynia, Cortex Phellodendri, honeysuckle, perilla leaf etc. play the role of antibacterial, anti-inflammatory, inhibit allergy, and Asian puccoon pair Inflammation caused by a variety of inflammation-causing substances is effective, it can reduce swelling, and reduces the amount of inflammatory exudate and the infiltration of inflammatory cell, in addition Asian puccoon also stablizes mast cell membrane, inhibits the effect of its release reaction, there are also good antiphlogistic effects for garden burnet, to prostaglandin Dermal microvessel permeability caused by E has very strong inhibiting effect, and can promote the healing of wound.External application garden burnet can also drop Low capillary vasopermeability reduces exudation, mitigates tissue edema, and have astriction, a large number of studies show that, Cortex Phellodendri, garden burnet, purple Grass is to staphylococcus aureus, hemolytic streptococcus, Diplococcus pneumopniae, corynebacterium diphtheriae, diplococcus meningitidis, Escherichia coli, wound Cold bacillus and Trichophyton rubrum etc. all have stronger bacteriostatic activity.The application effectively can treat and control infant eczema, mention High infant quality of life.

Overlay film saccharomycetes to make fermentation Product filtrate: the filtered fluid after being derived from the fermentation of Saccharomycopsis yeast, composition Substance includes amino acid, vitamin, minerals and carbohydrate lymphatic temperament.

Inventors have found that the overlay film saccharomycetes to make fermentation Product filtrate in the application not only has inhibition free radical, improve skin The effect of matter, and melanin can be inhibited, achieve the effect that whitening.Meanwhile the overlay film saccharomycetes to make fermentation product in the application Filtrate has the function of that long-acting moisturizing and accelerated ageing corneocyte update.In addition, inventor also found, the application In overlay film saccharomycetes to make fermentation product and plant extracts combine, can effectively treat or alleviate the symptom of eczema, lack Overlay film saccharomycetes to make fermentation product or plant extracts, therapeutic effect are remarkably decreased, inventor speculate itself the possible reason is, a side Face overlay film saccharomycetes to make fermentation product can make plant extracts be uniformly applied to the eczema position of skin, so that skin be made to fill Divide the ingredient that absorbs the drug, make local vasoconstriction, hyperemia is reduced, effectively mitigation inflammatory reaction；On the other hand, various plant extracts Effective component in object can protect overlay film saccharomycetes to make fermentation product and lack contaminated, holding drug effect.Inventor is also found surprisingly that, The application using special ratios overlay film saccharomycetes to make fermentation Product filtrate and plant extracts combine, the preparation being prepared without point Layer phenomenon, has good safety and stability.

Stearyl alcohol glycyrrhetin acid esters: stearyl alcohol glycyrrhetin acid esters is obtained due to having imported lipophilic high triacontanol, dissolubility Arrived significant improvement, low melting point has extensive compatibility in cosmetics, can be used for it is sun-proof, brighten, improve, it is antipruritic and protect It is wet, have the function of antibacterial, anti-inflammatory, anti-oxidant, anti-aging, absorption ultraviolet light, whitening skin lightening, nti-freckle etc..It is main in the product It is the effect for playing antibacterial anti-inflammatory.

Avocado oil: butter fruit is derived from, there is faint hazel seed taste, to cutaneous safety height, vitamin rich in, steroid Pure and mild lecithin etc. has good nutrition and osmosis, gives skin and adequately nourishes, and enables that skin and muscles are fair is careful glossy, Mainly play the role of skin care in the product.

Dipotassium glycyrrhizinate: dipotassium glycyrrhizinate is used as skin conditioning agent, predominantly the Radix Glycyrrhizae of Radix Glycyrrhizae root and stem Glucin (glycyrrhizic acid) ingredient is suitble to anaphylaxis skin, anti-irritant and oil-control, moves back red and swollen antiphlogistic healing effect.Skin can effectively be prevented Sensitivity inflammation phenomenon when stimulated.

Evening primrose oil: there is good elimination ability to superoxide radical, there is the effect of SOD sample, this is because γ-therein Caused by flax acid activity, it can be used for the antioxygen of cosmetics and anti-ageing；It can also be used in skin conditioning agent and smelly dose of suppression, be used for the product Mainly antibacterial and protection skin

Olibanum essential oil: the extraction of olibanum essential oil has a variety of medical values from olibanum resin, for skin, has anti-ageing Change, restore elasticity of skin, tighten pore, improve drying, inflammation, sensitive skin and other effects, have alleviation to infant eczema and controls The effect for the treatment of.

Bisabolol: bisabolol is extracted from spirea, plays the role of anti-inflammatory antibacterial, and the ingredient stability is good, and Skin has good compatibility, reduces scytitis, mitigates acne, prevents dark sore from generating, improve the anti-irritant energy of skin Power repairs the skin for having inflammation injury.

Aurantiamarin methyl chalcone: aurantiamarin methyl chalcone can enhance ascorbic effect, and have stronger antiviral And antibacterial action, the effect for leading to the tyrosinase of skin darkening can be inhibited, can be used for whitening and desalination blackspot, freckle.Have The normal permeability of blood vessel is maintained, capillary resistance is improved, enhances the elasticity and toughness of capillary, improves and prevent skin Red capillary.

The present invention is specifically described below by embodiment.It is necessarily pointed out that following embodiment is only used In the invention will be further described, it should not be understood as limiting the scope of the invention, professional and technical personnel in the field The some nonessential modifications and adaptations made according to the content of aforementioned present invention, still fall within protection scope of the present invention.

In addition, if without other explanations, it is raw materials used to be all commercially available.Such as it can be purchased from shop of Chinese medicines and Chinese Medicinal Materials Markets It can buy, specification meets national Chinese medicine standards of pharmacopoeia.Such as Herba Violae extract, dandelion extract, oldenlandia Careless extract, mint extract, phellodendron extract, coptis extract, Chinese rhubarb extract, Baical Skullcap root P.E, common cnidium fruit P.E, Atractylodes chinensis, Herba Schizonepetae extract, perilla leaf extract, Radix Sangusorbae extract, houttuynia extract, shrubby sophora extract can be purchased from Nanjing Dao Sifu Biotechnology Co., Ltd, specification 5:1；Nutgall extractive, polygonum cuspidate root extract are commercially available in Hunan Lang Lin Biological products Co., Ltd, number are respectively NAT-049, NAT-111；Armstrong extract, which can be purchased from Xi'an Tianrui biotechnology, to be had Limit company, specification 10:1；Shaggy-fruited dittany root bark extract is commercially available in Xi'an Rayleigh Biotechnology Co., Ltd, specification 30:1；Gold Honeysuckle flower extract can be purchased from Xinxiang Biotechnology Co., Ltd., and specification is chlorogenic acid 4%；The filter of overlay film saccharomycetes to make fermentation product Liquid can be purchased from Hangzhou Ou Shi Science and Technology Ltd..In addition all plant extracts and overlay film saccharomycetes to make fermentation Product filtrate be all It is recorded in " 2015 editions cosmetic material catalogues ".

Embodiment

Embodiment 1

1 one side of embodiment provides a kind of preparation, including active constituent D, component A, component B, component C；

By weight, the active constituent D includes following component: 1 part of overlay film saccharomycetes to make fermentation Product filtrate, plant mention Take 0.45 part of object；The plant extracts be Herba Violae extract, common cnidium fruit P.E, phellodendron extract, coptis extract, The combination of shaggy-fruited dittany root bark extract, polygonum cuspidate root extract, Armstrong extract, atractylodes chinensis, shrubby sophora extract, corresponding weight Part is than being 1:1:1:1:1:1:1:1:1.

By weight, the component A includes following component: 0.06 part of EDTA- disodium, 3 parts of glycerol, 2 parts of butanediol, Huang 0.03 part of virgin rubber, 0.02 part of low-acyl gellan gum, 0.02 part of high acyl gellan gum, 90 parts of water；

By weight, the component B includes following component: 2 parts of Glyceryl stearate citrate, stearyl alcohol glycyrrhetin 1 part of acid esters, 0.5 part of avocado oil, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of methyl hydroxybenzoate, 0.2 part of ethyl hydroxy benzoate；

By weight, the component C includes following component: 0.2 part of evening primrose oil, 0.2 part of olibanum essential oil, bisabolol 0.2 part, 0.1 part of aurantiamarin methyl chalcone, 0.05 part of tetrahydro piperine.

On the other hand the present embodiment additionally provides the preparation method of the preparation, include the following steps:

(1) the EDTA- disodium in component A is added to the water, is stirred to dissolve uniformly, then by the Huang in component A Virgin rubber, which is added in glycerol propylene glycol, stirs into uniform slurry, then slurry is added to the water, and be heated to 75 DEG C；

(2) B component is mixed and heated to 85 DEG C；

(3) component A is then stirred on one side, B component is added in A on one side, homogeneous 5 minutes, keep the temperature 20 minutes, then drop Temperature；

(4) after near 45 DEG C of temperature, component C is added and stirs evenly；

(5) after near 35 DEG C of temperature, active constituent D is added and stirs evenly；

Embodiment 2

2 one side of embodiment provides a kind of preparation, including active constituent D, component A, component B, component C；

By weight, the active constituent D includes following component: 1 part of overlay film saccharomycetes to make fermentation Product filtrate, plant mention Take 0.45 part of object；The plant extracts be houttuynia extract, Honegsukle flower P.E, nutgall extractive, shrubby sophora extract, The combination of mint extract, phellodendron extract, perilla leaf extract, Radix Sangusorbae extract, herba hedyotis diffusae extract, corresponding weight Amount part is compared for 1:1:1:1:1:1:1:1:1.

By weight, the component A includes following component: 0.06 part of EDTA- disodium, 3 parts of glycerol, 2 parts of butanediol, Huang 0.03 part of virgin rubber, 0.02 part of low-acyl gellan gum, 0.02 part of high acyl gellan gum, 90 parts of water；

By weight, the component B includes following component: 2 parts of Glyceryl stearate citrate, stearyl alcohol glycyrrhetin 1 part of acid esters, 0.5 part of avocado oil, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of methyl hydroxybenzoate, 0.2 part of ethyl hydroxy benzoate；

By weight, the component C includes following component: 0.2 part of evening primrose oil, 0.2 part of olibanum essential oil, bisabolol 0.2 part, 0.1 part of aurantiamarin methyl chalcone, 0.05 part of tetrahydro piperine.

On the other hand the present embodiment additionally provides the preparation method of the preparation, with embodiment 1.

Embodiment 3

3 one side of embodiment provides a kind of preparation, including active constituent D, component A, component B, component C；

By weight, the active constituent D includes following component: 1 part of overlay film saccharomycetes to make fermentation Product filtrate, plant mention Take 0.45 part of object；The plant extracts is Herba Violae extract, Chinese rhubarb extract, coptis extract, phellodendron extract, hardship The combination of conopsea extraction, dandelion extract, Herba Schizonepetae extract, Armstrong extract, atractylodes chinensis, corresponding weight part ratio are 5:3:5:5:5:5:5:5:5.

By weight, the component A includes following component: 0.06 part of EDTA- disodium, 3 parts of glycerol, 2 parts of butanediol, Huang 0.03 part of virgin rubber, 0.02 part of low-acyl gellan gum, 0.02 part of high acyl gellan gum, 90 parts of water；

By weight, the component B includes following component: 2 parts of Glyceryl stearate citrate, stearyl alcohol glycyrrhetin 1 part of acid esters, 0.5 part of avocado oil, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of methyl hydroxybenzoate, 0.2 part of ethyl hydroxy benzoate；

By weight, the component C includes following component: 0.2 part of evening primrose oil, 0.2 part of olibanum essential oil, bisabolol 0.2 part, 0.1 part of aurantiamarin methyl chalcone, 0.05 part of tetrahydro piperine.

On the other hand the present embodiment additionally provides the preparation method of the preparation, with embodiment 1.

Comparative example 1

1 one side of comparative example provides a kind of preparation, including active constituent D, component A, component B, component C；

By weight, the active constituent D be 1 part of overlay film saccharomycetes to make fermentation Product filtrate by weight, described group Dividing A includes following component: 0.06 part of EDTA- disodium, 3 parts of glycerol, 2 parts of butanediol, 0.03 part of xanthan gum, low-acyl gellan gum 0.02 part, 0.02 part of high acyl gellan gum, 90 parts of water；

By weight, the component B includes following component: 2 parts of Glyceryl stearate citrate, stearyl alcohol glycyrrhetin 1 part of acid esters, 0.5 part of avocado oil, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of methyl hydroxybenzoate, 0.2 part of ethyl hydroxy benzoate；

By weight, the component C includes following component: 0.2 part of evening primrose oil, 0.2 part of olibanum essential oil, bisabolol 0.2 part, 0.1 part of aurantiamarin methyl chalcone, 0.05 part of tetrahydro piperine.

On the other hand the present embodiment additionally provides the preparation method of the preparation, with embodiment 1.

Comparative example 2

2 one side of comparative example provides a kind of preparation, including active constituent D, component A, component B, component C；

By weight, the active constituent D is 0.45 part of plant extracts；The plant extracts mentions for viola mandshurica Object, common cnidium fruit P.E, phellodendron extract, coptis extract, shaggy-fruited dittany root bark extract, polygonum cuspidate root extract, Armstrong is taken to extract The combination of object, atractylodes chinensis, shrubby sophora extract, corresponding weight part ratio are 1:1:1:1:1:1:1:1:1.

By weight, the component A includes following component: 0.06 part of EDTA- disodium, 3 parts of glycerol, 2 parts of butanediol, Huang 0.03 part of virgin rubber, 0.02 part of low-acyl gellan gum, 0.02 part of high acyl gellan gum, 90 parts of water；

By weight, the component B includes following component: 2 parts of Glyceryl stearate citrate, stearyl alcohol glycyrrhetin 1 part of acid esters, 0.5 part of avocado oil, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of methyl hydroxybenzoate, 0.2 part of ethyl hydroxy benzoate；

By weight, the component C includes following component: 0.2 part of evening primrose oil, 0.2 part of olibanum essential oil, bisabolol 0.2 part, 0.1 part of aurantiamarin methyl chalcone, 0.05 part of tetrahydro piperine.

On the other hand the present embodiment additionally provides the preparation method of the preparation, with embodiment 1.

Comparative example 3

3 one side of comparative example provides a kind of preparation, including component A, component B, component C；

By weight, the component A includes following component: 0.06 part of EDTA- disodium, 3 parts of glycerol, 2 parts of butanediol, Huang 0.03 part of virgin rubber, 0.02 part of low-acyl gellan gum, 0.02 part of high acyl gellan gum, 90 parts of water；

By weight, the component B includes following component: 2 parts of Glyceryl stearate citrate, stearyl alcohol glycyrrhetin 1 part of acid esters, 0.5 part of avocado oil, 0.5 part of dipotassium glycyrrhizinate, 0.2 part of methyl hydroxybenzoate, 0.2 part of ethyl hydroxy benzoate；

By weight, the component C includes following component: 0.2 part of evening primrose oil, 0.2 part of olibanum essential oil, bisabolol 0.2 part, 0.1 part of aurantiamarin methyl chalcone, 0.05 part of tetrahydro piperine.

On the other hand the present embodiment additionally provides the preparation method of the preparation, include the following steps:

(1) the EDTA- disodium in component A is added to the water, is stirred to dissolve uniformly, then by the Huang in component A Virgin rubber, which is added in glycerol propylene glycol, stirs into uniform slurry, then slurry is added to the water, and be heated to 75 DEG C；

(2) B component is mixed and heated to 85 DEG C；

(3) component A is then stirred on one side, B component is added in A on one side, homogeneous 5 minutes, keep the temperature 20 minutes, then drop Temperature；

(4) after near 45 DEG C of temperature, component C is added and stirs evenly；

Performance test

1. stability test

Centrifugal test is carried out by preparation made from above-described embodiment 1- comparative example 3.Centrefuge experiment condition: 3000 revs/min, 30 minutes.

Test result: embodiment 1-3 is without lamination；All there is lamination in comparative example 1, comparative example 2, comparative example 3.

2. pharmacological activity is tested

Embodiment 1 is carried out referring to the LPS inducing mouse Macrophage Model in " anti-inflammatory effect of Daphniphyllum calycinum extract " Anti-inflammatory experiment.

(1), cell recovery

After being removed from liquid nitrogen macrophage (RAW264.7), 37 DEG C of water-baths are allowed to fast melt, to prevent the ice crystal of formation Damage cell.It is operated in Biohazard Safety Equipment below: the cell in cryopreservation tube is transferred to the disposable sterilized centrifuge tube of 15ml, 5ml DMEM culture medium is added and mixes (dual anti-containing 10% fetal calf serum and 1%), 1000rpm room temperature is centrifuged 4min, abandons supernatant, adds Enter 1ml DMEM culture medium and blow even precipitating, cell suspension is transferred to 10cm Tissue Culture Dish, 10ml DMEM culture is added Base, in 37 DEG C, 5%CO₂It is cultivated in incubator.Next day discards culture medium, after softly being washed with sterile PBS, replaces fresh cultured Base.

(2), cell passes on

After it is 90% that cell, which reaches density, culture medium is abandoned, with soft washing 2 times of sterile PBS, 1ml pancreatin is added, It is put into CO₂It in incubator, is observed under inverted microscope, if cell rounding, space between cells become larger, and naked eyes can see culture dish In cell start shedding off, then illustrate to have digested appropriateness, at this time be added 2ml culture medium terminate digestion, cell is blown and beaten, is blown Divide equally after even to 10cm Tissue Culture Dish, every ware is added 10ml DMEM culture medium, continues to cultivate in incubator.

(3), cell count

With pancreatin by after cell dissociation, 1000rpm is centrifuged 3min, abandons supernatant, and 3ml culture medium is added and is resuspended, draws 10 μ l Cell suspension is added in cell counting board, and tally is inserted into full-automatic cell calculating instrument, is obtained a result, and unit is a/ml, Two holes are surveyed simultaneously to be averaged.

(4), plating cells and administration

With 1*10 in 96 orifice plates⁴A/hole bed board, every hole culture medium 100uL, culture is long to 80% to cell, changes culture medium Sample to be tested, administration concentration 50,100,200,400ug/mL is added, 5ug/mL aminoguanidine is added in setting positive controls (AG), after 4h being administered, the LPS effect 16h of final concentration of 1ug/mL is added.In this step, sample to be tested is what embodiment 1 obtained Preparation is obtained with 0.9% physiological saline solution.

(5), sample detection

50uL culture medium supernatant is taken, is detected according to the method for NO kit: sample is added in 96 orifice plates by the hole 50uL/, The hole 50uL/ is pressed again, room temperature Griess Reagent I is added in each hole, then press the hole 50uL/, room temperature Griess is added in each hole Reagent II, with multi-function microplate reader wavelength be at 540nm measurement absorbance.

The anti-inflammatory experimental result of LPS inducing macrophage as shown in figure 5, show that embodiment 1 has good antiphlogistic effects, Significant dose-effect relationship is presented, anti-inflammatory activity is better than positive control drug aminoguanidine, this has also further proved its anti-inflammatory treatment Effect.

3. treating infant eczema measure of merit

Evaluation method: the formula is effective to treatment infant eczema.

Experimental method:

Choose 120 people of volunteer for suffering from skin eczema, age (agreement for obtaining its parent) between 1-8 years old.Male 60 People, 60 people of female.Male 20 people of the age between 1-2 years old, 20 people of women；Age between 3-5 years old, 18 people of male, women 22 people, male 21 people of the age between 6-8 years old, 19 people of women.6 groups are randomly divided into, every group of 20 people.

The grouping situation of 1 eczema treatment group of table

Administrated method:

Referring to the standard of " modern dermatopathy and venereal disease ", the skin eczema of selected volunteer meets acute, subacute or slow Property eczema diagnosis: 1. itch is significant, have exudation be inclined to, it is easy to recur；Skin lesion with erythema, mound sore rash, water scar, erosion based on be Acute eczema；

2. only a small number of papulo-vesicles, water scar, mild infiltration are subacute eczema based on small papule, the scales of skin that peel off, incrustation；

3. becoming master with pachyderma, coarse, the fresh sample of tongue fur is chronic eczema.

Every group of 20 people are randomly divided into 6 groups, and first group uses embodiment 1, and second group uses embodiment 2, and third group uses real Example 3 is applied, the 4th group uses embodiment 4, and the 5th group uses embodiment 5, and the 6th group uses embodiment 6；6 groups in gender, age, disease Journey and eczema position etc. compare, no significant difference, and respectively smearing affected part is primary in the morning, afternoon and evening daily by 6 groups of volunteers, and A few minutes are gently rubbed, continuous uninterrupted using 4 weeks, product can be stopped using by such as having fully recovered in 4 weeks, be sentenced according to therapeutic effect Constant current modulation is imitated and is compared.

The criterion of therapeutical effect of judgement

It is evaluated by 4 grades of criterions of therapeutical effect, is recovery from illness respectively, it is effective, it is effectively and invalid.Specific standards are as follows:

A recovery from illness: skin lesion is all subsided, and pruritis disappears, total effects rate >=90%；

B is effective: skin lesion is largely subsided, and itch is substantially reduced, 60%≤total effects rate < 90%；

C is effective: skin lesion partial remission, pruritis make moderate progress, 20%≤total effects rate < 60%；

D is invalid: skin lesion is subsided unobvious, and itch has no mitigation or clinical symptoms are counter sees deterioration, total effects rate < 20%

In terms of total effective rate adds obvious effective rate by cure rate

Therapeutic effect see the table below

Group	Number of cases	Recovery from illness	It is effective	Effectively	In vain	Total effective rate
							First group	20	12	6	1	1	95%
Second group	20	10	7	1	2	90%
							Third group	20	13	6	0	1	95%
4th group	20	0	3	4	13	35%
							5th group	20	0	4	5	11	45%
6th group	20	0	2	3	15	25%

Embodiment 1 is used according to the application method of this experiment, volunteer's picture before use is as shown in Figure 1；

Embodiment 1 is used according to the application method of this experiment, volunteer's picture after use is as shown in Figure 2；

Embodiment 2 is used according to the application method of this experiment, volunteer's picture before use is as shown in Figure 3；

Embodiment 2 is used according to the application method of this experiment, volunteer's picture after use is as shown in Figure 4；

From the point of view of test result, embodiment 1-3 provides a kind of preparation, has to the skin eczema disease of 1 to 8 year old infant Significant treatment and improvement, and it is environmentally protective, corticosteroid hormone and antibiotic are free of, therefore is not in hormone Side effect, also there is no crossed sensitization and crossing drug resistant is led to the problem of with other antibiotic, in addition this product matrix is not fine and smooth, dirty Dye clothing is readily cleaned, is curative for effect, for the skin eczema for treating infant, provides effective effect.

Embodiment above-mentioned is merely illustrative, and is used to explain the present invention some features of the method.Appended right It is required that the range as wide as possible for being intended to require to be contemplated that, and embodiments as presented herein is only according to all possible reality Apply the explanation of the embodiment of the combined selection of example.Therefore, the purpose of applicant is that the attached claims are not illustrated this The exemplary selectional restriction of the feature of invention.Some numberical ranges used also include son in the claims Range, the variation in these ranges should also be construed to be covered by the attached claims in the conceived case.

Claims (10)

1. a kind of composition for treating infant eczema, which is characterized in that including active constituent D, by weight, the activity Ingredient D includes following component: 0.5-3 parts of overlay film saccharomycetes to make fermentation Product filtrate, 0.1-1 parts of plant extracts.

2. a kind of composition for treating infant eczema according to claim 1, which is characterized in that including active constituent D, By weight, the active constituent D includes following component: 1 part of overlay film saccharomycetes to make fermentation Product filtrate, plant extracts 0.45 Part.

3. a kind of composition for treating infant eczema according to claim 1, which is characterized in that the plant extracts It is mentioned selected from Herba Violae extract, dandelion extract, herba hedyotis diffusae extract, mint extract, phellodendron extract, the coptis Take object, Chinese rhubarb extract, Baical Skullcap root P.E, common cnidium fruit P.E, nutgall extractive, atractylodes chinensis, polygonum cuspidate root extract, Herba Schizonepetae extract, Armstrong extract, shaggy-fruited dittany root bark extract, perilla leaf extract, Radix Sangusorbae extract, Honegsukle flower P.E, fish One of raw meat grass extract, shrubby sophora extract are a variety of.

4. a kind of composition for treating infant eczema according to claim 3, which is characterized in that the plant extracts It is extracted for Herba Violae extract, common cnidium fruit P.E, phellodendron extract, coptis extract, shaggy-fruited dittany root bark extract, polygoni cuspidati,radix The combination of object, Armstrong extract, atractylodes chinensis, shrubby sophora extract, corresponding weight part ratio are (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5).

5. a kind of composition for treating infant eczema according to claim 3, which is characterized in that the plant extracts For houttuynia extract, Honegsukle flower P.E, nutgall extractive, shrubby sophora extract, mint extract, phellodendron extract, purple The combination of perilla leaf extract, Radix Sangusorbae extract, herba hedyotis diffusae extract, corresponding weight part ratio are (1-5): (1-5): (1- 5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5).

6. a kind of composition for treating infant eczema according to claim 3, which is characterized in that the plant extracts For Herba Violae extract, Chinese rhubarb extract, coptis extract, phellodendron extract, shrubby sophora extract, dandelion extract, chaste tree The combination of mustard extract, Armstrong extract, atractylodes chinensis, corresponding weight part ratio are (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5).

7. a kind of preparation, which is characterized in that by including a kind of group for treating infant eczema described in any one of claims 1-6 It closes object to be prepared, dosage form is pellet, emulsus, any one in paste.

8. a kind of preparation according to claim 7, which is characterized in that further include component A, component B, component C；Wherein, it presses Parts by weight meter, the component A include following component: 0.1-0.1 parts of EDTA- disodium, 1-8 parts of glycerol, 1-5 parts of butanediol, xanthan 0.01-0.05 parts of glue, 0.01-0.05 parts of low-acyl gellan gum, 0.01-0.05 parts of high acyl gellan gum, 70-110 parts of water；It is described Component B includes following component: 1-3 parts of Glyceryl stearate citrate, 0.5-1.5 parts of stearyl alcohol glycyrrhetin acid esters, avocado oil 0.1-1 parts, 0.1-1 parts of dipotassium glycyrrhizinate, 0.1-0.5 parts of methyl hydroxybenzoate, 0.1-0.5 parts of ethyl hydroxy benzoate；The component C includes such as Lower component: 0.1-0.5 parts of evening primrose oil, 0.1-0.5 parts of olibanum essential oil, 0.1-0.5 parts of bisabolol, aurantiamarin methyl chalcone 0.1-0.5 parts, 0.01-0.1 parts of tetrahydro piperine.

9. a kind of preparation method of preparation according to claim 8, which comprises the steps of:

(1) the EDTA- disodium in component A is added to the water, is stirred to dissolve uniformly, then by the xanthan gum in component A It is added in glycerol propylene glycol and stirs into uniform slurry, then slurry is added to the water, and be heated to 70-80 DEG C；

(2) B component is mixed and heated to 80-90 DEG C；

(3) component A is then stirred on one side, B component is added in A on one side, homogeneous 5 minutes, keep the temperature 20 minutes, then cool down；

(4) after near 35-50 DEG C of temperature, component C is added and stirs evenly；

(5) after near 25-40 DEG C of temperature, active constituent D is added and stirs evenly.

10. a kind of application of composition for treating infant eczema according to claim 1-6, feature exist In the composition is for treating infant eczema.