CN114467525A - Method for improving tomato seedling resistance - Google Patents
Method for improving tomato seedling resistance Download PDFInfo
- Publication number
- CN114467525A CN114467525A CN202111620147.7A CN202111620147A CN114467525A CN 114467525 A CN114467525 A CN 114467525A CN 202111620147 A CN202111620147 A CN 202111620147A CN 114467525 A CN114467525 A CN 114467525A
- Authority
- CN
- China
- Prior art keywords
- tomato
- grafting
- resistance
- improving
- grafted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
Abstract
The invention relates to a method for improving tomato seedling resistance, which comprises the steps of grafting a tomato seedling onto a root of Chinese wolfberry for cultivation, and grafting the grafted tomato seedling onto another root of Chinese wolfberry for cultivation.
Description
Technical Field
The invention relates to the technical field of tomato planting, in particular to a method for improving tomato seedling resistance.
Background
The tomato, also called tomato, is called Diospyros kaki, and has rich nutrition and special flavor; the tomato has the effects of losing weight, eliminating fatigue, stimulating appetite, improving digestion of protein, reducing stomach distension and dyspepsia, and the like; when eating the tomato for diet therapy and weight reduction, the tomato takes mature juicy berries as the herbaceous plants of the product; every 100 g fresh fruit contains about 94 g water, 2.5-3.8 g carbohydrate, 0.6-1.2 g protein, 20-30 mg vitamin C, carotene, mineral salt, organic acid, etc.; is an enthusiastic vegetable and fruit for consumers.
The specification of "tomato seedling grafting technique and management method thereof" in patent application No. CN201510608462.6 describes "the tomato seedling grafting technique, which comprises the following steps: setting a seedling raising greenhouse, selecting a seedling raising matrix, selecting a stock, pre-treating and sowing seeds, a grafting method, an operation rule and a management method after grafting. The tomato grafted seedling provided by the invention adopts 'eggplant hybrid No. 2' as a stock, the disease resistance of the seedling is good, the early-stage sprouting vigor is strong, the stem of the eggplant stock is thick, the improvement of the survival rate of the grafted seedling is facilitated, meanwhile, the survival rate is improved in the actual grafting process by matching with the inarching technology and a later-stage optimized and reasonable management method, the tomato seedling grows well after being grafted, the later-stage fruit setting time is advanced, the fruit yield is high, the pulp color is pure, and the taste quality is good, so the commodity performance is high.
In summary, the research and development of a method for improving tomato seedling resistance still remains a key problem to be solved urgently in the technical field of tomato planting.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for improving the resistance of tomato seedlings, the method comprises the steps of grafting the tomato seedlings onto the roots of Chinese wolfberry for cultivation, and grafting the grafted tomato seedlings onto another root of Chinese wolfberry for cultivation, wherein the cultivated tomato seedlings have excellent cold resistance, and meanwhile, the cultivated tomato seedlings can combine the performance of Chinese wolfberry and have certain effect of relieving fatigue after being eaten.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for improving the resistance of tomato seedlings is characterized in that tomato seedlings are used as scions to be grafted on rootstocks to cultivate the tomato seedlings with resistance.
Further, the stock is the root of the Chinese wolfberry.
Further, the scion is a tomato seedling with 3-4 true leaves.
Further, the method comprises the steps of grafting tomato seedlings to the roots of the Chinese wolfberry fruits for cultivation, and grafting the grafted tomato seedlings to the other root of the Chinese wolfberry fruits for grafting cultivation.
Furthermore, the grafting cultivation is carried out in a time period from 4 months bottom to 5 months early.
Preferably, the medlar is Ningxia medlar or Zhonghua medlar.
Furthermore, the medlar is medlar which has 2 years or more, has no pathological changes and is cold-resistant.
In addition, the method also comprises the step of wrapping the grafting interface in a conventional mode.
Furthermore, the grafting connector is wrapped by a plastic bag.
Advantageous effects
Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:
the grafted tomato seedlings are grafted to another Chinese wolfberry root for grafting cultivation, the cultivated tomato seedlings have excellent cold resistance, can combine the performance of Chinese wolfberry, has a certain effect of relieving fatigue after being eaten, and has a wide application prospect.
Drawings
FIG. 1 is a statistical chart of the cold tolerance survival rate of tomato seedlings in example 1 of the present invention;
FIG. 2 is a statistical chart of the cold tolerance survival rate of tomato seedlings in example 2 of the present invention;
FIG. 3 is a statistical chart of the cold tolerance survival rate of tomato seedlings in example 3 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be further described with reference to the following examples.
Example 1: tomato seedlings are grafted by taking Ningxia wolfberry as stock
The specific method comprises the following steps: (1) grafting for the first time: planting tomato seeds into a seedling shed according to a conventional cultivation mode, and obliquely cutting downwards at an angle of 30-40 degrees at a position 3-5cm away from a base part of a seedling hypocotyl by using a cutting blade after 3-4 true leaves exist in the seedling, and taking the cut seedling as a scion; selecting the root of Ningxia wolfberry fruit of 2 years or more, no pathological change and cold resistance as stock, and cutting grafting port for grafting on the stock; grafting the scion onto the stock to complete the first grafting, wherein the grafting is carried out in the time period from 4 months bottom to 5 months early, and after the scion is grafted onto the stock, the grafting port can be wrapped by a plastic bag in a conventional mode; (2) and (3) second grafting: and grafting the grafted scion to the root of another 2-year or more disease-free and cold-resistant Ningxia wolfberry fruit for grafting cultivation.
The tomato seedlings grafted and cultivated by the method are used as an experimental group, meanwhile, according to the method, the branches and the stems of the Ningxia wolfberry are used as stocks, the tomato seedlings are used as scions, two times of grafting is carried out to be used as a comparison group, and the grafting process is the same as that of the experimental group.
100 tomatoes which are respectively grafted for the first time and the second time in the experimental group and 100 tomatoes which are grafted for the first time and the second time in the comparative group are planted in a cold-resistant area (the temperature is-12 ℃) in 4 months in 2020. The survival rates of the grafted tomatoes are compared and shown in table 1, and statistics on the related data are shown in fig. 1.
Table 1: cold-resistant survival rate table after tomato seedling grafting cultivation
| Grouping | Survival rate of first graft (%) | Survival rate of second graft (%) |
| |
92 | 99 |
| |
56 | 78 |
As can be seen from table 1 and fig. 1, the root of the ningxia wolfberry is used as the stock for grafting, and the cold-resistant survival rate of the tomato seedlings subjected to the second grafting reaches 99%, which indicates that the grafting survival rate of the tomatoes can be effectively improved by using the root of the ningxia wolfberry as the stock for grafting.
Meanwhile, the quality detection is carried out on the tomatoes in the experimental group and the comparative group, and the process is as follows:
selecting a male mouse with the weight of 18-22g, and dividing the mouse into a blank control group, a control experiment group and a control comparison group, wherein the control experiment group is fed with tomato fruits of the experiment group, the control comparison group is fed with tomato fruits of the comparison group, and the blank control group, the control experiment group and the control comparison group are all 15 mice; the anti-fatigue swimming test of the mice is carried out. After a 5% weight lead wire was loaded at the tail of the mouse, the mouse was put into a water tank (40 cm. times.60 cm. times.36 cm) for swimming, and the time from the start of swimming to the time of submerging the mouse (i.e., the swimming time of the mouse) was recorded on a stopwatch, and the experimental water temperature was 25 ℃ and the water depth was 20 cm. The results of the experiment are shown in table 2.
Table 2: influence table of mouse weight swimming time
The experimental results show that the swimming time of the mice of the control experimental group and the control comparative group is obviously prolonged (P is less than 0.01) compared with that of the blank control group, so that the tomato fruits obtained by grafting the tomato seedlings on the Ningxia wolfberry can play a role in relieving fatigue of the mice, and compared with the control comparative group, the control experimental group does not obviously prolong the heavy-load swimming time.
Example 2: tomato seedling grafted by adopting Chinese wolfberry as stock
The specific method comprises the following steps: (1) grafting for the first time: planting tomato seeds into a seedling shed according to a conventional cultivation mode, and obliquely cutting downwards at an angle of 30-40 degrees at a position 3-5cm away from a base part of a seedling hypocotyl by using a cutting blade after 3-4 true leaves exist in the seedling, and taking the cut seedling as a scion; selecting roots of Chinese wolfberry with no pathological changes and cold resistance for 2 years or more as stocks, and cutting grafting ports for grafting on the stocks; grafting the scion onto the stock to complete the first grafting, wherein the grafting is carried out in the time period from 4 months bottom to 5 months early, and after the scion is grafted onto the stock, the grafting port can be wrapped by a plastic bag in a conventional mode; (2) and (3) second grafting: and grafting the grafted scion to the root of the Chinese wolfberry of another 2 years or more, which has no pathological change and is cold-resistant, for grafting cultivation.
The tomato seedlings grafted and cultivated by the method are used as an experimental group, meanwhile, according to the method, the branches and the stems of Chinese wolfberry are used as stocks, the tomato seedlings are used as scions, two times of grafting is carried out to be used as a comparison group, and the grafting process is the same as that of the experimental group.
100 tomatoes which are respectively grafted for the first time and the second time in the experimental group and 100 tomatoes which are grafted for the first time and the second time in the comparative group are planted in a cold-resistant area (the temperature is-12 ℃) in 4 months in 2020. The survival rates of the grafted tomatoes were compared and shown in table 3, and statistics were performed on the relevant data and shown in fig. 2.
Table 3: cold-resistant survival rate table after tomato seedling grafting cultivation
| Grouping | Survival rate of first graft (%) | Survival rate of second graft (%) |
| |
90 | 98 |
| |
56 | 80 |
As can be seen from table 3 and fig. 2, the root of chinese wolfberry was grafted as the stock, and the cold tolerance survival rate of the tomato seedlings after the second grafting reached 98%, indicating that the grafting survival rate of tomatoes can be effectively improved by grafting with the root of chinese wolfberry as the stock.
Meanwhile, the quality detection is carried out on the tomatoes in the experimental group and the comparative group, and the process is as follows:
selecting a male mouse with the weight of 18-22g, and dividing the mouse into a blank control group, a control experiment group and a control comparison group, wherein the control experiment group is fed with tomato fruits of the experiment group, the control comparison group is fed with tomato fruits of the comparison group, and the blank control group, the control experiment group and the control comparison group are all 15 mice; the anti-fatigue swimming test of the mice is carried out. After a 5% weight lead wire was loaded at the tail of the mouse, the mouse was put into a water tank (40 cm. times.60 cm. times.36 cm) for swimming, and the time from the start of swimming to the time of submerging the mouse (i.e., the swimming time of the mouse) was recorded on a stopwatch, and the experimental water temperature was 25 ℃ and the water depth was 20 cm. The results of the experiment are shown in table 4.
Table 4: influence table of mouse weight swimming time
The experimental results show that the swimming time of the mice of the control experimental group and the control comparative group is obviously prolonged (P is less than 0.01) compared with that of the blank control group, so that the tomato fruits obtained by grafting the tomato seedlings on the Chinese wolfberry can play a role in relieving fatigue of the mice, and compared with the control comparative group, the control experimental group does not obviously prolong the heavy-load swimming time.
Example 3: comparison of the method provided by the invention with the prior art
Tomato seedlings were cultivated as an experimental group according to the method of example 1, and at the same time, tomato seedlings were cultivated as a control group by the method of patent application No. CN201510608462.6, and the cold-resistant survival rate of the grafted tomato seedlings was tested as shown in table 5, and statistics were made on the relevant data as shown in fig. 3, and then a mouse anti-fatigue swimming test was performed according to example 1 to test the fatigue-relieving effect of tomato fruits as shown in table 6.
Table 5: cold-resistant survival rate table after tomato seedling grafting cultivation
As can be seen from Table 5, the tomato seedlings cultivated by the method provided by the invention can effectively improve the grafting survival rate of tomatoes in a cold environment compared with the prior art.
Table 6: influence table of mouse weight swimming time
The experimental results show that the swimming time of the mice in the experimental group is obviously prolonged (P is less than 0.01) compared with that in the comparative group, so that the tomato fruits produced by the invention can play a role in relieving fatigue for the mice, and the comparative group is obviously inferior to the tomato fruits produced by the invention.
The method for improving the resistance of the tomato seedlings provided by the invention is characterized in that the tomato seedlings are firstly grafted to the roots of the Chinese wolfberry to be cultivated, and then the grafted tomato seedlings are grafted to the other roots of the Chinese wolfberry to be cultivated, so that the cultivated tomato seedlings have excellent cold resistance, and meanwhile, the cultivated tomato seedlings can combine the performance of the Chinese wolfberry and have a certain effect of relieving fatigue after being eaten.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (9)
1. A method for improving the resistance of tomato seedlings is characterized in that tomato seedlings are grafted to rootstocks to cultivate tomato seedlings with resistance as scions.
2. The method for improving tomato seedling resistance as claimed in claim 1, wherein the rootstock is root of Lycium chinense Miller.
3. The method for improving tomato seedling resistance as claimed in claim 1, wherein said scion is tomato seedling with 3-4 true leaves.
4. The method for improving the resistance of tomato seedlings according to claim 2, wherein the method comprises grafting tomato seedlings to the roots of Chinese wolfberry for cultivation, and grafting the grafted tomato seedlings to another root of Chinese wolfberry for cultivation.
5. The method for improving tomato seedling resistance as claimed in claim 1, wherein said grafting cultivation is performed in a period from 4 months bottom to 5 months early.
6. The method for improving tomato seedling resistance as claimed in claim 4, wherein the fructus Lycii is Ningxia fructus Lycii or Zhonghua fructus Lycii.
7. The method for improving tomato seedling resistance as claimed in claim 6, wherein the medlar is a medlar with 2 years or more, no pathological changes and cold resistance.
8. The method for improving tomato seedling resistance as claimed in claim 1, wherein said method further comprises wrapping said graft interface in a conventional manner.
9. The method for improving tomato seedling resistance as claimed in claim 1, wherein said wrapping is by wrapping the grafting base with a plastic bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111620147.7A CN114467525A (en) | 2021-12-23 | 2021-12-23 | Method for improving tomato seedling resistance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111620147.7A CN114467525A (en) | 2021-12-23 | 2021-12-23 | Method for improving tomato seedling resistance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114467525A true CN114467525A (en) | 2022-05-13 |
Family
ID=81496639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111620147.7A Pending CN114467525A (en) | 2021-12-23 | 2021-12-23 | Method for improving tomato seedling resistance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114467525A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542011A (en) * | 2015-01-19 | 2015-04-29 | 贵州广贵育苗场 | Method for improving disease resistance of black tomatoes though grafting method |
| CN105123472A (en) * | 2015-08-28 | 2015-12-09 | 寿光市新世纪种苗有限公司 | Method for cultivating healthy grafting seedlings of tomatoes |
| CN106376357A (en) * | 2016-08-24 | 2017-02-08 | 石新法 | Matrimony vine tomato grafting method |
| CN107896672A (en) * | 2017-12-15 | 2018-04-13 | 四川农业大学 | A kind of continuous grafting of tomato is used for the method for its offspring's cadmium pollution reparation |
| CN108370737A (en) * | 2018-03-08 | 2018-08-07 | 河北农业大学 | A kind of scion suitable for solanaceous vegetables grafting regenerates breeding method |
| JP2020068710A (en) * | 2018-10-31 | 2020-05-07 | 国立大学法人名古屋大学 | Rootstock for tomato which can be used for improving tomato fruit sugar content and/or stress resistance |
| CN111357514A (en) * | 2020-03-06 | 2020-07-03 | 光泽县富民蔬菜专业合作社 | Tomato double-stock grafting method |
| CN113057032A (en) * | 2021-03-22 | 2021-07-02 | 大连金谷园林绿化工程有限公司 | Cross-family/cross-genus/cross-species grafting method for dicotyledonous plants |
-
2021
- 2021-12-23 CN CN202111620147.7A patent/CN114467525A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542011A (en) * | 2015-01-19 | 2015-04-29 | 贵州广贵育苗场 | Method for improving disease resistance of black tomatoes though grafting method |
| CN105123472A (en) * | 2015-08-28 | 2015-12-09 | 寿光市新世纪种苗有限公司 | Method for cultivating healthy grafting seedlings of tomatoes |
| CN106376357A (en) * | 2016-08-24 | 2017-02-08 | 石新法 | Matrimony vine tomato grafting method |
| CN107896672A (en) * | 2017-12-15 | 2018-04-13 | 四川农业大学 | A kind of continuous grafting of tomato is used for the method for its offspring's cadmium pollution reparation |
| CN108370737A (en) * | 2018-03-08 | 2018-08-07 | 河北农业大学 | A kind of scion suitable for solanaceous vegetables grafting regenerates breeding method |
| JP2020068710A (en) * | 2018-10-31 | 2020-05-07 | 国立大学法人名古屋大学 | Rootstock for tomato which can be used for improving tomato fruit sugar content and/or stress resistance |
| CN111357514A (en) * | 2020-03-06 | 2020-07-03 | 光泽县富民蔬菜专业合作社 | Tomato double-stock grafting method |
| CN113057032A (en) * | 2021-03-22 | 2021-07-02 | 大连金谷园林绿化工程有限公司 | Cross-family/cross-genus/cross-species grafting method for dicotyledonous plants |
Non-Patent Citations (2)
| Title |
|---|
| 河南农学院蔬菜教研室, 河南科学技术出版社 * |
| 王久兴: "《蔬菜栽培关键技术与经验》", 30 June 2017, 中国科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101720647B (en) | Cultivation method for increasing sugar content of green loquat fruits | |
| CN103004550A (en) | Cultivation method for three-dimensionally interplanting grapes and strawberries | |
| KR100532072B1 (en) | New citrus variety asexually propagated by grafting | |
| CN108719029A (en) | A kind of grape without the matrix hydroponic method | |
| CN114467525A (en) | Method for improving tomato seedling resistance | |
| CN106857045B (en) | High-lighting-effect light and simple wax apple shaping and trimming method | |
| CN111670799B (en) | Sweet corn breeding method | |
| CN111670802B (en) | Breeding method of long-white chicken leg type green Chinese onions | |
| CN1253066C (en) | Germination method for furred trichosanthes seed | |
| CN108934739B (en) | Cultivation method for delaying grape maturation period | |
| CN106171960B (en) | A kind of selection of fringe portion without wax malting barley variety | |
| Wakana et al. | ‘Bea-Kei’, a new triploid seedless grape cultivar derived from a ‘Muscat Bailey A’בKyoho’cross | |
| CN106416759A (en) | Agate lycium barbarum culturing method | |
| CN104719132B (en) | A kind of selection of the white skin radish of the oval tubular green grass or young crops shoulder of spring resistance to bolting | |
| CN111802150B (en) | Method for improving quality of Jianyang tangelo | |
| CN113557903B (en) | Method for cultivating late-maturing kiwi fruit variety with stripes and with cold resistance and low sugar and fruit fragrance | |
| CN107787779A (en) | A kind of breeding method of the red seed melon of multipurpose | |
| CN112715088B (en) | Chenopodium quinoa willd seed reserving method | |
| CN107637441B (en) | Cultivation method of regenerated aromatic tobacco | |
| CN108849304B (en) | Cucumber and Chinese cabbage co-cultivation method | |
| Nguyễn et al. | Breeding new F1 hybrids cucumber for the Southeastern market in Vietnam | |
| Onika et al. | The mobilization and conservation of Diospyros virginiana l. Plants in NBGI | |
| Luthra | KUFRI THAR-2: A NEW DROUGHT TOLERANT TABLE POTATO VARIETY | |
| CN112167049A (en) | Method for cultivating novel variety of grapes, namely Xueshan rose grapes | |
| Solanki et al. | Crop improvement scenario in guava. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |