CN114451351A - Artificial breeding method of cuora flavomarginata - Google Patents

Artificial breeding method of cuora flavomarginata Download PDF

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CN114451351A
CN114451351A CN202210063475.XA CN202210063475A CN114451351A CN 114451351 A CN114451351 A CN 114451351A CN 202210063475 A CN202210063475 A CN 202210063475A CN 114451351 A CN114451351 A CN 114451351A
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turtles
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洪孝友
朱新平
魏成清
庄家然
刘晓莉
王亚坤
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Pearl River Fisheries Research Institute CAFS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/02Breeding vertebrates

Abstract

The invention provides an artificial breeding method of yellow-forehead closed-shell turtles, which comprises the following steps: cultivating the yellow-forehead closed shell turtle in a simulated wild environment, feeding fungi, artificial compound protein feed and fresh fruits and vegetables daily, and atomizing and spraying tap water to promote appetite before feeding; in the mating period, the content of animal protein in food is increased, male and female turtles are artificially paired, and tap water is sprayed in an atomized manner to promote mating; collecting the turtle eggs, and carrying out artificial hatching on the fertilized eggs. By adopting the breeding method, the annual egg laying amount of the yellow-rated box turtles is 0.92, the fertility rate is 54.17%, the hatchability is 75.72%, the egg laying amount of the yellow-rated box turtles is effectively improved, the hatchability is further improved, the artificial high-efficiency propagation of the yellow-rated box turtles is realized, the scale of the existing artificial propagation population is enlarged, and the species protection is promoted.

Description

Artificial breeding method of cuora flavomarginata
Technical Field
The invention belongs to the technical field of yellow-frontal box turtle breeding, and particularly relates to an artificial breeding method of yellow-frontal box turtles.
Background
Cohole balanopsis flavomarginata (scientific name: Cuora galbinifrons) is a reptile of the genus Cohole tortoise, the family of the turtle family. It is only distributed in Guangxi and Hainan provinces in China. With the development of economy and the demand of turtle ornamental markets, the field habitat fragmentation and hunting phenomenon lead to the gradual decline and death of the field population of the yellow-head shell turtle. The yellow-fronted closed-shell turtles are recorded as extremely dangerous species by red names of Chinese vertebrates, aquatic wild animals are protected for the II-level in China, and the appendix II of international trade convention of endangered wild animals and plants is included.
The yellow-fronted cockchafer is fond of inhabiting streams and shallow water areas in hilly and mountainous areas and is often hidden in fallen leaves under forests. The land tends to be strong, and the swimming pool can often move in shallow water and is not good for swimming. The cuora flavomarginata is generally very small in size and difficult to feed, and food refusal is common in field-captured cuora flavomarginata. The artificial cultivation is only in the provinces of Hainan, Guangdong, etc. According to incomplete statistics, more than 500 yellow-rated box turtle artificial breeding certificates and more than 5 million artificial breeding certificates exist in Guangdong province, but the breeding death rate is high, meanwhile, the yellow-rated box turtle breeding technology is extremely immature, only few breeding units can utilize wild breeding turtles to be bred to generate a first filial generation, and the egg laying amount, the fertility rate and the hatching rate are all extremely low. At present, only 1 case of yellow-fronted box turtle artificially pre-fed for 3 years is reported to lay eggs with the egg laying amount of 0.6 per year, the fertility rate of 55.56 percent and the hatchability of 60 percent (Huangxinsheng, 2020).
The requirements of the yellow-fronted closed-shell turtles on water temperature, bait, breeding density, mating, breeding environment conditions and the like in the artificial breeding process are greatly different from those of other freshwater turtles and are difficult to master, so that the artificially-bred yellow-fronted closed-shell turtles die greatly, do not lay eggs for many years, have small egg laying amount, low fertility rate, low hatching rate and the like, and the artificial domestication and protection of the species are severely limited.
Disclosure of Invention
The invention provides an artificial breeding method of yellow-frontal box turtles, which can effectively improve the egg laying amount of the yellow-frontal box turtles, further improve the hatchability and realize the artificial efficient breeding of the yellow-frontal box turtles.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention provides an artificial breeding method of yellow-forehead closed-shell turtles, which comprises the following steps:
the yellow-forehead closed shell turtle is cultivated in a simulated wild environment, fungi, artificial compound protein feed and fresh fruits and vegetables are fed daily, and tap water is sprayed in an atomized mode before feeding to promote appetite; in the mating period, the content of animal protein in food is increased, male and female turtles are manually paired, and tap water is sprayed in an atomized manner to promote mating; collecting the tortoise eggs, and carrying out artificial incubation on the fertilized eggs.
Preferably, when the yellow-forehead box turtle is artificially introduced or rescued, the yellow-forehead box turtle needs to be separately pre-bred for 2-3 months.
Preferably, the yellow-rated box turtle needs to be pre-raised before being cultured in a wild environment, only water is fed in the first 10-20 days of pre-raising, and then the yellow-rated box turtle is fed once every three days, wherein the feeding amount of food for each time is 1-3% of the weight of the turtle.
Preferably, the simulated wild environment comprises land and a water pool, and the land is paved by soil, dead leaves and bark in a mixing way.
Preferably, the land is planted with green plants, and the coverage rate of the green plants accounts for more than 80% of the land area.
Preferably, before feeding, the atomized spraying tap water is sprayed for 10-20 min, and the spraying amount is 1.5-2.5L/h per square meter.
Preferably, the mating phase is increased weekly by feeding suckling mice or barley worms.
Preferably, the paired male and female turtles are selected from male and female turtles which are 6 years old or older, have a healthy and strong constitution of 750g or more, have no diseases, have no picky eating condition and have obvious sinking feeling.
Preferably, when the male and female turtles are matched manually, the spraying amount is 3.5-4.5L/h per square meter, and tap water is sprayed in an atomized manner for 0.5-1.5 h.
Preferably, the female turtles are bred in a combined mode, and the density is 1-2 turtles/m2
Compared with the prior art, the invention has the following beneficial effects:
the breeding method can effectively solve the technical problems in the artificial breeding process of the yellow-rated box turtles, effectively improve the breeding survival rate, the egg laying amount and the hatching rate of the yellow-rated box turtles, realize the artificial high-efficiency breeding of the yellow-rated box turtles, enlarge the scale of the existing artificial breeding population and promote species protection.
The breeding method is low in cost, easy to operate and suitable for popularization of artificial breeding of the yellow-forehead Stephania mandshurica.
Detailed Description
The invention provides an artificial breeding method of yellow-forehead closed-shell turtles, which comprises the following steps:
the yellow-forehead closed shell turtle is cultivated in a simulated wild environment, fungi, artificial compound protein feed and fresh fruits and vegetables are fed daily, and tap water is sprayed in an atomized mode before feeding to promote appetite; in the mating period, the content of animal protein in food is increased, male and female turtles are manually paired, and tap water is sprayed in an atomized manner to promote mating; collecting the tortoise eggs, and carrying out artificial incubation on the fertilized eggs.
In the invention, when the yellow-forehead box turtle is artificially introduced or rescued, the yellow-forehead box turtle is preferably pre-raised for 2-3 months, and more preferably for 2 months. In the invention, the pre-feeding is carried out in a round or square plastic basin, the size is preferably 10 times of the area of the tortoise back shell, the height is about 30cm, the tortoise cannot climb out, then a proper box is used for placing a drinking basin, the water is changed every day, and the changed water is tap water which is placed for one day. The temperature of the pre-feeding environment is 20-30 ℃.
In the invention, the pre-feeding is preferably to feed only water for the first 10-20 days, and then to feed the water once for three days, the food feeding amount per time is preferably 1-3% of the weight of the tortoise, more preferably to feed only water for the first 15 days of the pre-feeding, and the food feeding amount per time is more preferably 2% of the weight of the tortoise. In the invention, the food is preferably fungus, artificial compound feed and fresh fruits and vegetables, the fungus comprises one or more of oyster mushroom, pleurotus geesteranus, shiitake mushroom and straw mushroom, the artificial feed comprises feed with protein content of more than 41%, and the fresh fruits and vegetables comprise one or more of Chinese cabbage, sweet potato leaves, bananas, durian and apples. As an implementation mode, the feeding is carried out once in three days, the food of the turtle is the fungus containing oyster mushrooms and pleurotus geesteranus and the artificial formula feed with the protein content of more than 41%, the weight ratio of the fungus to the artificial formula feed is 1:1, and fresh fruits and vegetables containing one or more of Chinese cabbage, sweet potato leaves, bananas, durian and apples are not fed every week. In the present invention, after observing the excretion of the turtle twice, the turtle was orally administered once a day for three consecutive days using a manicure file (20 mg/kg).
In the present invention, the simulated wild environment preferably includes land and water ponds, the land being paved with a mixture of soil, dead leaves, and bark. In the invention, the mat material keeps about 70% of humidity, the thickness of the mat material in summer is 5cm, and the thickness of the mat material in winter is 15-20 cm. In the invention, the land area accounts for 80%, and the pool accounts for 20%. According to the method, green plants are planted on the land, the coverage rate of the green plants accounts for more than 80% of the area of the land, the green plants are preferably evergreen trees, and the evergreen trees are preferably one or more of litchi trees, mango trees, waxberry trees, orange trees and Japanese pines. In the invention, the water depth of the pool is 5-10 cm, water is changed every day, and the changed water is tap water placed for one day. The wild environment simulated feeding temperature is 20-30 ℃, and the environment needs to be ventilated and fresh.
In the invention, the female turtles after being pre-raised for 2 months are put into a simulated wild environment for combined cultivation, and the density is preferably 1-2/m2The male turtles can be cultured in plastic pots alone or in a simulated wild environment alone. In the invention, the female turtles are from wild, and are cultured in a wild environment for more than 2 years.
In the invention, before feeding, the atomized spraying tap water is preferably sprayed for 10-20 min, more preferably 15min, and the spraying amount is preferably 1.5-2.5L/h per square meter, more preferably 2L/h per square meter. In the invention, the feeding is carried out by taking fungi containing one or more of oyster mushroom, pleurotus geesteranus, shiitake mushroom and straw mushroom and artificial compound feed with more than 41 percent of protein content as food of the turtles, wherein the weight ratio of the fungi to the artificial compound feed is 1:1, and fresh fruits and vegetables containing one or more of Chinese cabbage, sweet potato leaves, bananas, durian and apples are not fixedly fed every week; the feeding amount of the food in each time is preferably 1-3% of the weight of the tortoise, and more preferably 2% of the weight of the tortoise.
In the invention, the paired male and female turtles are selected from male and female turtles which are 6 years old or more, have the health and health of 750g or more, have no diseases, have no picky eating condition and have obvious sinking feeling.
In the invention, the air temperature is stabilized at 17-18 ℃ or higher in spring in the mating period, and in the mating period, besides normal feeding, the content of animal protein in food is increased by feeding 10g of suckling mice or barley insects every week.
In the invention, after 2-3 weeks of continuous feeding of suckling mice or barley worms, each 2 female turtles are individually separated by using a plastic plate or a ceramic chip and 1 mature male turtle is placed in the artificial pairing.
In the invention, when the male and female turtles are matched manually, the atomized spraying tap water is preferably 0.5-1.5 h, more preferably 1h, and the spraying amount is preferably 3.5-4.5L/h per square meter, more preferably 4L/h per square meter. According to the invention, most male and female turtles can complete mating within 2-3 hours of atomizing and spraying tap water. In the invention, the male and female individuals are separated artificially after mating is successful, the female individuals continue to be co-cultured, and the male individuals are raised independently; if the fact that the male and female turtles fight violently is observed to indicate that mating is unsuccessful, the male and female individuals are separated manually and cultured independently for 1 week, then different male and female individuals are selected to artificially promote mating according to the artificial pairing method, and if the mating is unsuccessful, the trial is conducted again in the 2 nd year.
In the invention, the collection of the tortoise eggs is carried out in 4-6 months per year. In the invention, the yellow-fronted box turtle lays eggs for 1-2 years, and the eggs are produced once or twice in 1 day. In the invention, the turtle eats eggs or destroys the eggs during egg laying, and pond patrol work is needed in the egg laying season, or infrared monitoring is installed, so that egg laying is discovered and timely collected for artificial incubation of fertilized eggs.
In the invention, the fertilized eggs are preferably incubated in a transparent white incubation box, and the length, width and height of the incubation box are preferably 20-25 × 12-16 × 6-10 cm, and more preferably 22 × 14 × 8 cm. In the invention, vermiculite is used as a hatching medium in the hatching box, wet vermiculite with the thickness of 3-6 cm is firstly paved in the hatching box, and then 2cm of dry vermiculite is paved on the wet vermiculite. In the invention, the wet vermiculite is vermiculite which is fully absorbed with water and drained for 2 hours, and the proportion of the vermiculite to the water is about 1: 1.3. The air humidity in the hatching box is 75-85%.
In the present invention, the method for artificial hatching of fertilized egg comprises the following steps: putting 2 fertilized eggs into the center of a transparent white hatching box, wherein the distance between the 2 eggs is about 5-6 cm; covering the box cover, putting the box cover into an incubator, incubating at 20-28 ℃, and taking the young yellow-forehead closed-shell turtles out of the shells within about 80-100 days.
In the present invention, all the components are commercially available products well known to those skilled in the art unless otherwise specified.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
1. Pre-feeding: the yellow-forehead closed shell turtles which are artificially introduced or rescued are independently raised in a plastic basin, are round or square, are preferably 10 times the area of tortoise back shells in size, and are preferably not crawled out when the height is about 30 cm; the drinking water basin is arranged in a proper box, water is changed every day, and the changed water is tap water placed for one day. The temperature of the feeding environment is controlled to be 20-30 ℃, and the feed can be fed after 15 days. Feeding once in three days, taking fungus containing oyster mushroom and pleurotus geesteranus and artificial mixed feed (with the protein content of more than 41%) as food of turtles, wherein the weight ratio of the fungus to the artificial mixed feed is 1:1, and the feeding amount of the food every time is 2% of the weight of turtles. Fresh fruits and vegetables including cabbage, sweet potato leaf, banana, durian, apple are not fed every week. After observing the excretion of the turtle twice, the turtle was orally taken once a day for three consecutive days using a Nitrocellus file (20 mg/kg). The male and female are raised separately for 2 months.
2. A breeding environment: land area accounts for 80%, and the pool accounts for 20%. The land is paved with soil, dead leaves and barks in a mixed manner, wherein the thickness of the soil is 5cm in summer and 15-20cm in winter; the green plants are selected from evergreen trees (such as litchi trees and mango trees), and the coverage rate of the green plants is more than 80% of the land area. The mat material keeps about 70% of humidity, and the environment needs to be ventilated and fresh. The water depth of the pool is 5-10 cm, water is changed every day, and the changed water is tap water placed for one day. The temperature of the breeding environment is controlled to be 20-30 ℃.
Putting the female turtles which are pre-bred for 2 months into a combined breeding mode, wherein the density is 1-3/m2. If the female turtles are wild source, the female turtles are cultured under the environment condition for more than 2 years. The male turtles can be cultured in separate plastic pots or separatelyCulturing in the above culture environment.
3. Daily management: feeding once every two days, and spraying tap water for 15min by atomization before feeding, wherein the spraying amount is 2L/h per square meter so as to improve the eating desire; fungus containing oyster mushroom and pleurotus geesteranus and artificial compound feed (the protein content is more than 41%) are used as food of turtles, the weight ratio of the fungus to the artificial compound feed is 1:1, and the feeding amount of the food in each time is 2% of the weight of the turtles. Fresh fruits and vegetables including cabbage, sweet potato leaf, banana, durian, apple are not fed every week.
4. Selecting breeding turtles: selecting male and female turtles with the age of more than 6, the constitution of more than 750g, no diseases, no picky eating condition and obvious sinking feeling.
5. Manual pairing: the temperature is stabilized to be more than 17-18 ℃ in spring, besides normal feeding, 10g of suckling mice or barley insects are fed every week to improve the content of animal protein in food, after continuous feeding is carried out for 2-3 weeks, every 3 female turtles are separated independently by using plastic plates or ceramic tiles, after 1 mature male turtles are placed, tap water is sprayed for 1h in an atomized mode, the spraying amount per square meter is 4L/h, and most of the female turtles and the male turtles can complete mating within 2-3 hours after water is sprayed. Observing that the male and female individuals are separated artificially after mating is successful, the female individuals continue to be co-cultured, and the male individuals are raised independently; if the mating is not successful by observing that the male and female turtles fight violently, the male and female individuals are separated manually and cultured independently for 1 week, then different male and female individuals are selected to artificially promote mating according to the method, and if the mating is not successful, the trial is carried out again in the 2 nd year.
6. Collecting turtle eggs: the spawning period of the yellow-frontal closed-shell turtles is 4-6 months, the yellow-frontal closed-shell turtles spawn 1-2 eggs every year, and the turtles eat eggs or destroy the eggs after the eggs are produced once or twice in 1 day. And (3) making pond patrol work in the spawning season, or installing infrared monitoring, finding spawning, collecting in time, and performing artificial incubation of fertilized eggs.
7. Hatching: hatching the fertilized eggs in a transparent white hatching box, wherein the length, the width and the height of the hatching box are 22 multiplied by 14 multiplied by 8 cm; taking vermiculite as a hatching medium, firstly laying vermiculite with a thickness of 4cm in a hatching box, fully absorbing water, draining the vermiculite for 2 hours (at the moment, the specific gravity of the vermiculite and the water is about 1:1.3), then laying 2cm of dry vermiculite on the box, controlling the air humidity to be 75-85%, placing 2 fertilized eggs in the center of the box, and keeping the distance between the 2 eggs to be about 5-6 cm. Covering the box cover, putting the box cover into an incubator, incubating at 25 ℃, and taking the young yellow-forehead closed-shell turtles out of the shells in about 80-100 days.
Example 2
12 female wild yellow-rated cockchafers and 4 male wild yellow-rated cockchafers are cultivated according to the method in the embodiment 1, the cockchafers are artificially simulated in 2018-2019 for 2 years in a wild environment, and are bred in pairing manner in 2020 and 2021, so that breeding data are obtained as shown in the following table 1, the egg laying amount of the yellow-rated cockchafers in 2 years is 0.92, the fertilization rate is 54.17%, and the hatching rate is 75.72%.
TABLE 12020-year incubation data of Anemone polyphylla in 2021
Figure BDA0003479306530000061
Figure BDA0003479306530000071
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. An artificial breeding method of yellow-forehead closed-shell turtles is characterized by comprising the following steps:
the yellow-forehead closed shell turtle is cultivated in a simulated wild environment, fungi, artificial compound protein feed and fresh fruits and vegetables are fed daily, and tap water is sprayed in an atomized mode before feeding to promote appetite; in the mating period, the content of animal protein in food is increased, male and female turtles are manually paired, and tap water is sprayed in an atomized manner to promote mating; collecting the tortoise eggs, and carrying out artificial incubation on the fertilized eggs.
2. The cultivation method of claim 1, wherein the yellow-fronted turtle is artificially introduced or rescued, and is pre-bred for 2-3 months.
3. The cultivation method according to claim 2, wherein the water is only fed for the first 10-20 days of the pre-feeding, and the water is fed once for three days later, and the amount of the fed food is 1-3% of the weight of the tortoise each time.
4. The method of claim 1, wherein the simulated wildlife environment comprises land and water ponds, the land being paved with a mixture of soil, dead leaves, and bark.
5. The cultivation method according to claim 4, wherein the land is planted with green plants, and the coverage of the green plants accounts for more than 80% of the land area.
6. The cultivation method according to claim 1, wherein the atomized spray of tap water is carried out for 10-20 min before feeding, and the spray amount is 1.5-2.5L/h per square meter.
7. The breeding method of claim 1, wherein the mating period is increased weekly for feeding suckling mice or barley worms.
8. The breeding method according to claim 1, wherein the paired male and female turtles are selected from male and female turtles with age of 6 or more, health and health of 750g or more, no disease, no food preference and obvious sinking sensation.
9. The cultivation method of claim 1, wherein when the male and female turtles are matched manually, tap water is sprayed in an atomized manner for 0.5 to 1.5 hours, and the spraying amount is 3.5 to 4.5L/h per square meter.
10. The cultivation method according to claim 1, wherein the female turtles are cultivated in a combined manner and the density is 1-2 turtles/m2
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