CN114423779A - 口蹄疫病毒疫苗组合物 - Google Patents
口蹄疫病毒疫苗组合物 Download PDFInfo
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- CN114423779A CN114423779A CN202080061233.6A CN202080061233A CN114423779A CN 114423779 A CN114423779 A CN 114423779A CN 202080061233 A CN202080061233 A CN 202080061233A CN 114423779 A CN114423779 A CN 114423779A
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Abstract
本发明涉及一种参与引发对口蹄疫病毒的免疫应答的多肽、多核苷酸以及包含它们的组合物。本发明还涉及一种引发受试者对口蹄疫病毒的免疫应答的方法。本发明还涉及一种用于预防口蹄疫的药物组合物，其包含参与引发对口蹄疫病毒的免疫应答的多肽、多核苷酸或质粒。此外，本发明涉及一种在受试者中预防口蹄疫的方法。
Description
技术领域
本发明涉及参与引发对口蹄疫病毒的免疫应答的多肽、多核苷酸、质粒以及包含它们的疫苗组合物。本发明还涉及在受试者中引发对口蹄疫病毒的免疫应答的方法。本发明还涉及用于预防口蹄疫的药物组合物，其包含参与引发对口蹄疫病毒的免疫应答的多肽、多核苷酸或质粒。此外，本发明涉及一种在受试者中预防口蹄疫的方法。
背景技术
口蹄疫(Foot-and-Mouth Disease,FMD)是一种影响偶蹄动物(偶蹄目Artiodactyla)的病毒性疾病，包括牛、猪、绵羊、山羊、鹿等家畜和各种野生动物，其特征是在嘴巴、鼻子、乳头、蹄子等处形成水泡。口蹄疫具有高度传染性，死亡率高达40％或以上，这使其成为一种具有社会经济影响的疾病。为此，世界动物卫生组织(OIE)将FMD归类为A类疾病并进行管理，在大多数国家，FMD已被定为一类传染病。此外，在过去十年中，几乎每个大陆都发生了FMD疫情，造成了严重的经济损失。
口蹄疫病毒(FMDV)是一种正义、单链RNA病毒，属于小核糖核酸病毒科(Picornaviridae)的口蹄疫病毒属(Aphthovirus)。它不仅侵袭性强，而且感染范围广。其潜伏期可能因动物而异，但短至约6至10天。此外，由于它在出现任何临床症状之前已经从体内排出，因此很容易发生传播。它通过呼吸系统、消化系统、生殖系统等传播。特别是通过呼吸系统释放的病毒可以通过空气传播长达250公里。
根据VP1编码区的序列，FMDV可分为O、A、C、SAT1、SAT2、SAT3和亚洲1(Asia 1)7个血清型，VP1在结构蛋白基因区中具有最多的突变，并且对于确定免疫原性(如中和抗体的形成)很重要。此外，根据遗传特征，每个血清型可分为多达约80个亚型。
在亚洲(包括韩国、东南亚)和欧洲，O血清型的FMD最为常见，因此针对O血清型的FMD疫苗已被积极研究和接种。然而，迄今为止开发的针对O血清型的FMD疫苗并不能有效地防御最近在包括韩国在内的亚洲出现的口蹄疫病毒。因此，迫切需要开发一种针对O血清型FMDV的新型疫苗。
另一方面，由于主要发生在韩国的口蹄疫是O血清型，因此对A血清型病毒的准备不足。然而，2017年2月在韩国延川市发生的口蹄疫为A血清型，这使得情况变得严重。
此外，已知Asia 1血清型在口蹄疫血清型中相对较弱，但自2005年中国出现Asia1血清型口蹄疫以来，Asia 1型在东亚地区开始不断出现(Hammond J.2009.OIE/FAO世界参考实验室报告,1月-3月2009,口蹄疫.世界参考实验室,皮尔布赖特:1-33)。这些属于基因型V的病毒，被证实与在印度分离的病毒有遗传关系(Jong hyun Park等,2008a,"世界口蹄疫分子流行病学分析及近期分布(Molecular epidemiological analysis and recentdistribution of foot-and-mouth disease in the world)",预防兽医学杂志)。考虑到韩国周边地区口蹄疫的现状，除了A血清型和O血清型外，还需要为Asia1血清型进入韩国的情况做好准备。
针对特定血清型病毒的疫苗不具有针对其他血清型病毒的免疫力，并且存在属于同一血清型的两个毒株的DNA序列差异高达总基因的30％的情况。因此，由于FMDV有多种血清型和亚型，疫苗应针对每种血清型单独应用。此外，由于一种血清型存在许多突变，因此很难开发出有效的疫苗。而且，即使接种口蹄疫疫苗，也存在免疫力只能维持几个月的缺点。
如上所述，针对一种血清型口蹄疫的疫苗对另一种血清型没有免疫力，因此应接种二价或更高的多价疫苗，以防止两种或多种病毒同时出现。因此，需要能够通过一次接种产生针对两种或更多种血清型的抗原的多价疫苗。
发明内容
技术问题
本发明一个目的是提供一种能够引发针对口蹄疫病毒的免疫应答的多肽，以及编码该多肽的多核苷酸。
本发明的另一个目的是提供一种能够引发对口蹄疫病毒的免疫应答的质粒。
本发明的另一个目的是提供一种口蹄疫病毒疫苗组合物。
本发明的另一个目的是提供一种在受试者中引发对口蹄疫病毒的免疫应答的方法。
本发明的另一个目的是提供一种预防口蹄疫的药物组合物。
本发明的另一个目的是提供一种在受试者中预防口蹄疫的方法。
技术方案
为了实现上述目的，本发明一方面提供了一种多肽，其包含SEQ ID NO：1的氨基酸序列或与其具有90％以上同一性的氨基酸序列。SEQ ID NO:1的氨基酸序列或与其具有90％以上序列同一性的氨基酸序列可以是对应于O血清型口蹄疫病毒的VP1区的序列。
如本文所用，术语“同一性”是指与给定多肽序列或多核苷酸序列对应的程度，并且可以以百分比表示。在本说明书中，与给定多肽序列具有相同或相似活性的序列的同一性可以用“％同一性”表示。同一性可以通过使用标准软件来确定，标准软件例如BLAST2.0，该软件可以计算诸如分数、同一性、相似性等参数，或通过在严格定义的条件下使用杂交测试比较序列来确定。待定义的合适的杂交条件可以通过本领域技术人员熟知的任何方法来确定。
SEQ ID NO：1的氨基酸序列是通过最近在亚洲出现的O血清型口蹄疫病毒的VP1重组获得的。包含SEQ ID NO:1的氨基酸序列或与其具有90％以上同一性的氨基酸序列的多肽可以有效地充当能够引发针对口蹄疫病毒、特别是O血清型口蹄疫病毒的免疫应答的抗原。
根据本发明的另一方面，本发明提供了一种多肽，其包含SEQ ID NO：1的氨基酸序列或与其具有90％以上同一性的氨基酸序列，以及口蹄疫病毒的VP2至VP4蛋白的氨基酸序列。
优选地，在所述多肽中，SEQ ID NO：1的氨基酸序列或与其具有90％以上同一性的氨基酸序列可以连接到对应于源自口蹄疫病毒的VP2、VP3和VP4蛋白的氨基酸序列。例如，它们可以以VP4区氨基酸序列—VP2区氨基酸序列—VP3区氨基酸序列—SEQ ID NO：1氨基酸序列的顺序连接。VP2至VP4蛋白的氨基酸序列可优选来源于口蹄疫病毒，特别是O血清型口蹄疫病毒。
每个氨基酸序列之间可以包括额外的氨基酸序列。例如，在每个VP区域的序列之间可以包含弗林蛋白酶切割位点(furin cleavage site)的氨基酸序列。例如，弗林蛋白酶切割位点可以是序列RGRKRRS、序列AGGGGAAGAAAGAGGAGAAGC或序列AGGGGACGCAAGAGGCGCTCA。
此外，所述多肽还可包含Kozak序列、IgE前导序列和/或2A氨基酸序列。例如，所述多肽可以包含IgE前导序列—VP4区的氨基酸序列—VP2区的氨基酸序列—VP3区的氨基酸序列—SEQ ID NO：1的氨基酸序列—2A的氨基酸序列(优选按上述顺序)。在这种情况下，优选地，在每个区域的序列之间可以进一步包括弗林蛋白酶切割位点的氨基酸序列。2A氨基酸序列可以是例如对应于核苷酸序列ACCCTGAACTTCGACCTCCTCAAACTG GCTGGCGATGTGGAAAGCAATCCTGGATGATAA的氨基酸序列。
此外，多肽还可以包括针对两种或更多种不同口蹄疫病毒血清型产生免疫应答的多肽。例如，多肽可以包括针对选自O、A、C、SAT1、SAT2、S AT3和Asia 1血清型口蹄疫病毒中的2、3、4、5、6或7种血清型产生免疫应答的多肽。
作为二价疫苗的一个具体实例，该多肽可以包含IgE前导序列-A血清型FMDV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区-A血清型FMDV的VP1区-O血清型FMDV的VP4区-O血清型FMDV的VP2区-O血清型FMDV的VP3区-O血清型FMDV的VP1区-2A氨基酸序列。每个区域的序列之间还可以进一步包含弗林蛋白酶切割位点的氨基酸序列，例如IgE前导序列-A血清型FMDV的VP4区-弗林蛋白酶切割位点-A血清型FMDV的VP2区-弗林蛋白酶切割位点-A血清型FMDV的VP3区-弗林蛋白酶切割位点-A血清型FMDV的VP1区-弗林蛋白酶切割位点-O血清型FMDV的VP4区-弗林蛋白酶切割位点-O血清型FMDV的VP2区-弗林蛋白酶切割位点-O血清型FMDV的VP3区-弗林蛋白酶切割位点-O血清型FMD V的VP1区-弗林蛋白酶切割位点-2A氨基酸序列。这种类型的二价疫苗可能对O和A血清型的FMDV具有免疫力。
作为三价疫苗的一个具体实例，该多肽可以包含IgE前导序列-A血清型FMDV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区-A血清型FMDV的VP1区-O血清型FMDV的VP4区-O血清型FMDV的VP2区-O血清型FMDV的VP3区-O血清型FMDV的VP1区-Asia1血清型F MDV的VP4区-Asia1血清型FMDV的VP2区-Asia1血清型FMDV的VP3区-Asia1血清型FMDV的VP1区-2A氨基酸序列。在各区域的序列之间还可以进一步包括弗林蛋白酶切割位点的氨基酸序列，例如IgE前导序列-A血清型FMDV的VP4区-弗林蛋白酶切割位点-A血清型FMDV的VP2区-弗林蛋白酶切割位点-A血清型FMDV的VP3区-弗林蛋白酶切割位点-A血清型FMDV的VP1区-弗林蛋白酶切割位点-O血清型FMDV的VP4区-弗林蛋白酶切割位点-O血清型FMDV的VP2区-弗林蛋白酶切割位点-O血清型FMDV的VP3区-弗林蛋白酶切割位点-O血清型FMDV的VP1区-弗林蛋白酶切割位点-Asia1血清型FMDV的VP4区-弗林蛋白酶切割位点-Asia1血清型FMDV的VP2区-Asia1血清型FMDV的VP3区-Asia1血清型FM DV的VP1区-弗林蛋白酶切割位点-2A氨基酸序列。这种类型的三价疫苗可能对O、A和Asia1血清型FMDV具有免疫力。
优选地，在二价或三价疫苗中，O血清型FMDV的VPl区包含SEQ I DNO：1的氨基酸序列。
优选地，在二价或三价疫苗中，A血清型FMDV是在申请人的其他韩国专利申请No.10-2018-0149242(申请日：2018年11月28日)中公开的序列。
作为一个实例，在二价或三价疫苗中，A血清型FMDV的VPl区包含S EQ ID NO：85或86的氨基酸序列。SEQ ID NOs：85和86是对应于A血清型FMDV的VP1区的重组序列。
作为另一个实例，在二价或三价疫苗中，A血清型FMDV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区可以是SEQ ID NO：87的氨基酸序列或包含SEQ ID NO：87的氨基酸序列。这里，弗林蛋白酶切割位点的氨基酸序列可以进一步包含在每个VP区域之间。
本发明的一个方面提供了一种多核苷酸，其包含SEQ ID NO：2的核苷酸序列或与其具有90％以上同一性的核苷酸序列。
或者，本发明的另一个方面提供了一种编码SEQ ID NO：1的氨基酸序列的多核苷酸。编码SEQ ID NO：1的氨基酸序列的多核苷酸可以是SE Q ID NO：2的核苷酸序列。
或者，编码SEQ ID NO：1的氨基酸序列的多核苷酸可以是如下序列，其中SEQ IDNO：2的核苷酸序列被修饰为针对要引发免疫应答的动物优化的密码子。该动物可以是任何可能发生或发展FMD的动物，例如猪、牛、羊等，并且优选是猪。
本发明的一个方面提供了一种包含多核苷酸的质粒，所述多核苷酸包含SEQ IDNO：2的核苷酸序列或与其具有90％以上同一性的核苷酸序列。
根据本发明的另一个方面，本发明提供了一种质粒，所述质粒包含编码SEQ IDNO：1的氨基酸序列或与SEQ ID NO：1的氨基酸序列具有90％以上同一性的氨基酸序列的多核苷酸。
术语“质粒”是指一种DNA构造体，该构造体含有与合适的调控序列可操作地连接的DNA序列，该调控序列能够在适当宿主中表达DNA。质粒可以是载体、噬菌体颗粒或仅仅是潜在的基因组插入物。在本说明书中，术语质粒可以与术语载体或病毒载体互换使用。
质粒可进一步包含编码口蹄疫病毒的VP2至VP4蛋白的核苷酸序列。质粒可进一步包括编码口蹄疫病毒的VP2至VP4蛋白的核苷酸序列。编码VP2至VP4蛋白的核苷酸序列可优选来源于口蹄疫病毒，特别是O血清型口蹄疫病毒。
在质粒中包含SEQ ID NO：2的核苷酸序列或与其具有90％以上同一性的核苷酸序列的多核苷酸可以与编码口蹄疫病毒的VP2至VP4蛋白的核苷酸序列连接，并且优选地可以与编码源自口蹄疫病毒的VP2、VP3和VP4蛋白的核苷酸序列依次连接。例如，质粒可以包含以VP4区的核苷酸序列-VP2区的核苷酸序列-VP3区的核苷酸序列-SEQ ID NO：2的核苷酸序列(在5'到3'方向上)的顺序连接的多核苷酸。
在每个区域的核苷酸序列之间可以包括额外的核苷酸序列。额外的核苷酸序列可以是对应于弗林蛋白酶切割位点区域的核苷酸序列。例如，弗林蛋白酶切割位点可以是序列AGGGGAAGAAAGAGGAGAAGC或AGGGGAC GCAAGAGGCGCTCA。
作为一个优选的实例，该质粒可以包含SEQ ID NO：3的核苷酸序列。
此外，该质粒还可以包含Kozak序列、IgE前导序列和/或2A序列。例如，该质粒可以包含IgE前导序列-VP4区的核苷酸序列-VP2区的核苷酸序列-VP3区的核苷酸序列-SEQ IDNO：2所示的核苷酸序列-2A核苷酸序列(优选地，以上述顺序)。2A核苷酸序列可以是例如核苷酸序列ACCCTG AACTTCGACCTCCTCAAACTGGCTGGCGATGTGGAAAGCAATCCTGGA TGATAA。
此外，质粒可包含针对两种或更多种不同口蹄疫病毒血清型产生免疫应答的多核苷酸。例如，它可以包括针对选自O、A、C、SAT1、SAT2、SAT3和Asia1血清型口蹄疫病毒中的2、3、4、5、6或7种血清型产生免疫应答的多核苷酸。
作为二价疫苗的具体实例，多核苷酸可包含IgE前导序列-A血清型FM DV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区-A血清型FMDV的VP1区-O血清型FMDV的VP4区-O血清型FMDV的VP2区-O血清型FMDV的VP3区-O血清型FMDV的VP1区-2A核苷酸序列。在每个区域的序列之间可以进一步包括弗林蛋白酶切割位点的核苷酸序列，例如IgE前导序列-A血清型FMDV的VP4区-弗林蛋白酶切割位点-A血清型FMDV的VP2区-弗林蛋白酶切割位点-A血清型FMDV的VP3区-弗林蛋白酶切割位点-A血清型FMDV的VP1区-弗林蛋白酶切割位点-O血清型F MDV的VP4区-弗林蛋白酶切割位点-O血清型FMDV的VP2区-弗林蛋白酶切割位点-O血清型FMDV的VP3区-弗林蛋白酶切割位点-O血清型FMD V的VP1区-弗林蛋白酶切割位点-2A核苷酸序列。这种类型的二价疫苗可能对O和A血清型FMDV具有免疫力。
作为三价疫苗的具体实例，多核苷酸可包含IgE前导序列-A血清型FM DV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区-A血清型FMDV的VP1区-O血清型FMDV的VP4区-O血清型FMDV的VP2区-O血清型FMDV的VP3区-O血清型FMDV的VP1区-Asia1血清型FMDV的VP4区-Asia1血清型FMDV的VP2区-Asia1血清型FMDV的VP3区-A血清型FMDVsia1的VP1区-2A核苷酸序列。在每个区域的序列之间可以进一步包括弗林蛋白酶切割位点的核苷酸序列，例如IgE前导序列-A血清型F MDV的VP4区-弗林蛋白酶切割位点-A血清型FMDV的VP2区-弗林蛋白酶切割位点-A血清型FMDV的VP3区-弗林蛋白酶切割位点-A血清型FMD V的VP1区-弗林蛋白酶切割位点-O血清型FMDV的VP4区-弗林蛋白酶切割位点-O血清型FMDV的VP2区-弗林蛋白酶切割位点-O血清型FMDV的VP3区-弗林蛋白酶切割位点-O血清型FMDV的VP1区-弗林蛋白酶切割位点-Asia1血清型FMDV的VP4区-弗林蛋白酶切割位点-Asia1血清型FMDV的VP2区域-Asia1血清型FMDV的VP3区域-Asia1血清型FMDV的VP1区域-弗林蛋白酶切割位点-2A核苷酸序列。这种类型的三价疫苗可能对O、A和Asia1血清型FMDVs具有免疫力。
优选地，在二价或三价疫苗中，O血清型FMDV的VP1区包含SEQ I D NO：1的氨基酸序列。
优选地，在二价或三价疫苗中，A血清型FMDV是在申请人的其他韩国专利申请No.10-2018-0149242(申请日：2018年11月28日)中公开的序列。
作为一个实例，在二价或三价疫苗中，A血清型FMDV的VP1区包含SEQ ID NO：88、89或90的核苷酸序列。SEQ ID NOs：88、89和90所示的核苷酸序列分别是对应于A血清型FMDV的VP1区的重组序列。特别地，SEQ ID NO：89的核苷酸序列是SEQ ID NO：88的核苷酸序列用猪密码子优化后的序列。
作为另一个实例，在二价或三价疫苗中，A血清型FMDV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区可以是或包含SEQ I D NO：91的核苷酸序列。这里，弗林蛋白酶切割位点的核苷酸序列可以进一步包含在每个VP区域之间。
作为另一个实例，在二价或三价疫苗中，A血清型FMDV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区-A血清型FMDV的VP1区可以是或包含SEQ ID NO：92的核苷酸序列。这里，弗林蛋白酶切割位点的核苷酸序列可以进一步包含在每个VP区域之间。
作为另一个实例，在二价或三价疫苗中，Asia1血清型FMDV的VP4区-A血清型FMDV的VP2区-A血清型FMDV的VP3区-A血清型FMDV的VP1区可以是或包含SEQ ID NO：94的核苷酸序列。这里，弗林蛋白酶切割位点的核苷酸序列可以进一步包含在每个VP区域之间。
根据本发明的另一方面，提供了一种用于FMD的疫苗组合物，其包含本发明所述的多肽、多核苷酸或质粒。
除非另有说明，FMD疫苗组合物中的多肽、多核苷酸和质粒与上述相同。
术语“疫苗”是指含有抗原的生物制剂，可使受试者免疫。即，疫苗是指免疫原性或抗原性物质，例如通过注射或口服给予人或动物以预防感染，从而为受试者提供免疫。
该疫苗可以是DNA疫苗。术语“DNA疫苗”是指通过人为地复制病原体、病毒等的某些基因并对其进行施用而引起免疫应答的疫苗。这些DNA疫苗与现有蛋白质疫苗相比具有许多优势，包括如下优势：(i)DNA疫苗可以仅利用纯目标病原体抗原的遗传信息即可合成，因此无需处理有害病原体本身，(ii)由于仅使用了一些诱导毒性所需的基因，因此DNA疫苗在施用于受试者时不会显示任何意外毒性，(iii)由于质粒DNA的结构简单，可以通过对突然发生的各种传染病的快速反应来开发疫苗。
用于FMD的疫苗组合物可以对FMDV，特别是O血清型FMDV具有保护性免疫。
本发明的疫苗组合物可以包含兽医学上可接受的载体。术语“兽医学上可接受的载体”包括任何和所有溶剂、分散介质、涂层、免疫佐剂、稳定剂、稀释剂、防腐剂、抗菌剂、抗真菌剂、等渗剂、吸附延迟剂等。可包含在疫苗组合物中的载体、赋形剂和稀释剂的实例包括乳糖、葡萄糖、蔗糖、山梨糖醇、甘露糖醇、木糖醇、麦芽糖醇、淀粉、甘油、阿拉伯胶、海藻酸盐、明胶、磷酸钙、硅酸钙、纤维素、甲基纤维素、微晶纤维素、聚乙烯吡咯烷酮、水、羟基苯甲酸甲酯、羟基苯甲酸丙酯、滑石粉、硬脂酸镁和矿物油。另外，本发明的疫苗组合物可以按照常规方法制成口服剂型，例如散剂、颗粒剂、片剂、胶囊剂、混悬剂、乳剂、糖浆剂、气雾剂等或无菌注射液。当配制疫苗组合物时，可以使用任何稀释剂或赋形剂制备组合物，例如常用的填充剂、增量剂、粘合剂、润湿剂、崩解剂、表面活性剂等。特别优选地，可以使用聚-L-谷氨酸帮助稳定。
疫苗组合物可以通过多种形式注射到受试者体内。“注射”可以通过选自皮下注射、肌肉注射、皮内注射、腹腔注射、鼻内给药、静脉内注射、经皮给药和口服给药所组成的组中的任一种来进行。
疫苗组合物可以包含一种或多种佐剂以改善或增强免疫应答。合适的佐剂包括肽、氢氧化铝、磷酸铝、氧化铝、矿物油如Marcol 52、植物油、乳化剂或表面活性物质如溶血卵磷脂、聚阳离子、聚阴离子等。
本发明的一个方面提供了一种针对两种或更多种血清型的FMDV的多价疫苗组合物。
疫苗组合物对其具有保护性免疫的FMDV可以是两种或更多种，例如，对选自O、A、C、SAT1、SAT2、SAT3和Asia1血清型FMDV中的2、3、4、5、6或7种。作为一个具体的实例，FMDV可以是O和A血清型，在这种情况下，疫苗组合物作为二价疫苗发挥作用。作为另一个实例，FMDV可能是O、A和Asia1血清型，在这种情况下，疫苗组合物作为三价疫苗发挥作用。
根据本发明的另一个方面，它提供了一种引发受试者对FMDV的免疫应答的方法，包括：向受试者施用本发明的疫苗组合物。
除非另有说明，在引发免疫应答的方法中的疫苗组合物与上述相同。
术语“免疫应答”是指响应抗原的引入而激活宿主的免疫系统，所述宿主例如是哺乳动物。免疫应答的形式可以是细胞免疫应答和/或体液免疫应答。
术语“受试者”是指包括人在内的动物，并且可以是，例如哺乳动物、有蹄类动物，特别是猪、牛、羊等。它可以与个体互换使用。
在引发免疫应答的方法中，所述疫苗组合物可以包含有效量的活性成分，例如重组多肽、重组多核苷酸或包含它们的质粒，以及药学上可接受的载体和/或佐剂。术语“有效量”是指疫苗成分的量足以在接种疫苗的动物中诱导针对FMDV的特异性免疫反应。本领域技术人员可以容易地确定有效量，例如通过对动物的典型实验来确定。
给药可以通过适于施用DNA疫苗的任何途径进行，并且例如可以通过皮下、肌肉内、腹膜内或静脉内注射进行。
优选地，所述FMDV为O血清型FMDV。
本发明的另一方面提供了一种在受试者中预防FMD的方法，优选为预防O血清型FMD的方法，该方法包括向受试者施用本发明的疫苗组合物。
本发明的另一方面提供了一种用于预防FMD的药物组合物，优选为预防O血清型FMD的药物组合物，该组合物包含本发明的多肽、多核苷酸或质粒。
本发明的预防FMD的方法和预防FMD的药物组合物中，除非另有说明，各术语具有与上述相同的含义。
术语“预防”或“防止”是指通过施用本发明的组合物抑制或延迟FM D的任何作用。
本发明的效果
本发明所述的疫苗组合物可以提供对FMDV显示出高免疫原性的DNA疫苗组合物。如果施用本发明的疫苗组合物，则在接种疫苗的个体中产生的针对FMDV的抗体水平非常高，因此有效地表现出免疫应答。特别是对O血清型FMDV具有极好的保护性免疫应答，特别是最近在亚洲出现的O血清型FMDV。
因此，当对猪或牛等个体给药时，可以实现极好的预防FMD的效果。
此外，本发明所述的疫苗组合物可以与其他血清型FMD的疫苗、抑制剂或用于治疗或预防其他血清型FMD药剂组合使用。或者，它可以用作多价疫苗以预防两种或多种血清型FMD。
附图说明
图1显示了通过替换O血清型FMD DNA疫苗VP1的部分氨基酸制备的重组VP1氨基酸序列(SEQ ID NO：1)。
图2显示了通过替换O血清型FMD DNA疫苗VP1的部分氨基酸制备的重组VP1核苷酸序列(SEQ ID NO：2)。
图3是利用新的重组VP1核苷酸序列替换现有O血清型FMD DNA疫苗的VP1区的过程示意图。
图4显示了PLS-FMD-O DNA疫苗的部分序列和区域。
图5显示了本发明所述疫苗在小鼠中施用后的抗体形成效果的SPC ELISA分析结果。
图6显示了本发明所述二价DNA疫苗是有效对抗A和O血清型FMD有效的多价疫苗的示例。
图7显示了本发明所述二价疫苗在小鼠中施用后的抗体形成效果的SPC ELISA分析结果。
图8显示了本发明所述三价DNA疫苗是有效对抗A、O和Asia1血清型FMD的多价疫苗的示例。
图9显示了本发明的三价疫苗在小鼠中施用后的抗体形成效果的SPC ELISA分析结果。
具体实施方式
以下，通过实施例更详细地说明本发明。然而，这些实施例仅用于说明本发明，本发明的范围不限于此。
实施例1.FMDV血清型O的序列分析
WRLFMD(世界口蹄疫参考实验室)网站，ME报告中(分子流行病学报告)对最近在亚洲出现的O血清型FMDV的VP1区进行了研究。
FMDV的VP1区在FMDV的P1结构蛋白基因区中突变最多，是决定免疫原性的重要位点，例如中和抗体的形成。
该研究在21个国家和地区进行，包括孟加拉国、不丹、柬埔寨、中国、印度尼西亚、印度、日本、老挝、马来西亚、蒙古、缅甸、尼泊尔、菲律宾、俄罗斯、新加坡、韩国、斯里兰卡、泰国和越南。
(1)柬埔寨
表1
(2)中国
表2
(3)中国香港
表3
(4)印度尼西亚：1986年宣布无FMD
(5)日本
表4
(6)老挝
表5
(7)马来西亚
表6
(8)蒙古
表7
(9)缅甸
表8
(10)菲律宾：自2005年12月以来没有记录到FMD病例
(11)俄罗斯
表9
(12)新加坡：自1973年无口蹄疫
(13)韩国
表10
(14)中国台湾
表11
(15)泰国
表12
(16)越南
表13
(17)孟加拉国
表14
| 年份 | 病毒 | 登记号 | ％Id | 拓扑型 | 世系 |
| 2009 | BAN/1/2009 | HQ630676 | 100％ | ME-SA | Ind-2001 |
(18)不丹
表15
(19)印度：无
(20)尼泊尔
表16
(21)斯里兰卡
表17
从ME报告结果中，本发明选择了核苷酸序列信息可以在NCBI GeneBank中找到的病毒株。结果，总共选出了81个病毒株，然后将这些病毒的VP1核苷酸序列转化为氨基酸序列。SEQ ID NOs：4至84分别分配给所选病毒的VP1氨基酸序列。
表18
实施例2.新型DNA疫苗的制备
通过Clustal/muscle对SEQ ID NOs：4至84的81个氨基酸序列进行比对。根据比对结果，推导出VP1的共有(consensus)序列。将衍生的共有序列与常规的O血清型FMD DNA疫苗pGX9014的序列进行比较，结果证实存在5个氨基酸错配。
在现有O血清型FMD DNA疫苗pGX9014的VP1区域中，替换了与共有序列不匹配的五个氨基酸。图1显示通过替换得到的VP1的氨基酸序列(SEQ ID NO：1)，图2显示了通过替换获得的VP1的核苷酸序列(SEQ ID NO:2)。
使用由SEQ ID NO:2的核苷酸序列所示的多核苷酸制备了一种新型FMD血清型O疫苗，整体示意图如图3所示。如图3所示，制备的重组核苷酸中，Msc I位点、VP3 C-末端位点、弗林蛋白酶切割位点、由SEQ ID NO：2的核苷酸序列表示的重组VP1位点、弗林蛋白酶切割位点、2A和Xho I位点顺序连接。所制备的重组核苷酸的序列和位点如图4所示，由SEQ IDNO：3表示。包含SEQ ID NO：3的核苷酸的DNA质粒被命名为“PLS-FMD-O”。
将具有PLS-FMD-O的大肠杆菌接种于2.5L的LB培养基中，然后在37℃下过夜培养。然后使用EndoFree Plasmid Giga试剂盒(QIAGEN,Cat#12391)从培养的大肠杆菌中提取质粒。将提取的质粒溶解在无内毒素水中并储存在-70℃下。然后将储存的质粒用于抗体形成实验，以确认动物的疫苗接种效果。
实施例3.新型DNA疫苗的抗体形成效应
3.1 DNA疫苗接种
将15只C57BL/6小鼠(8周龄，雌性)分成三组，每组5只，如下所示，每组每隔两周接种本发明疫苗、常规疫苗和模拟质粒3次。使用电穿孔装置(cellectra 2000)进行接种，DNA疫苗以30μg/小鼠的浓度给药。
·第1组(对照组)-模拟质粒DNA
·第2组(对照组)-O血清型FMD DNA(pGX1414，常规疫苗)
·第3组(实验组)-O血清型FMD DNA(PLS-FMD-O，本发明疫苗)
在给药前(第0周)、第二次给药后(第4周)和第三次给药后(第6周)使用眼眶后采血法采集血液。从收集的血液中分离血清并在-80℃下储存。将储存的血清溶解并进行SPC(固相竞争)ELISA分析。
3.2.测量抗体形成-SPC ELISA分析
根据试剂盒制造商提供的实验方案，使用PrioCHECK A血清型FMDV ELISA试剂盒(Prionics lelystad B.V/荷兰)进行SPC ELISA分析。
结果如图5所示。图5中可见，本发明的疫苗PLS-FMD-O具有比现有疫苗pGX1414更高的PI(抑制百分比)值。此处，PI值越高，产生的针对O血清型FMDV的特异性抗体就越多。与现有疫苗pGX1414相比，本发明的疫苗PLS-FMD-O在同一时间产生的抗体明显更高。
如上所述，FMD具有高度传染性，并且由于FMD病毒在出现任何临床症状之前就已经从体内排出，因此很容易发生动物间的传播。而且FMD的潜伏期非常短。因此，为了发挥作为FMD疫苗的有效功能，不仅要抑制FMD病毒的效果优异，而且要快速产生针对FMD病毒的抗体。在施用根据本发明的疫苗的情况下，该疫苗不仅在抑制FMD病毒方面非常出色，而且产生抗体的速度也明显快于传统疫苗，因此有可能在早期阶段抑制FMD的传染。因此，根据本发明的疫苗是一种非常有效的针对FMD的疫苗。
实施例4.新型DNA二价疫苗的制备
使用先前制备的重组序列制备针对A和O血清型FMD的二价DNA疫苗。
图6显示了制备二价疫苗的示例。在二价疫苗中，A血清型FMD的VP1区可能包含SEQID NO：88、89或90的核苷酸序列，O血清型FMD的VP1区可能包含SEQ ID NO：2的核苷酸序列。
使用SEQ ID NO：89的核苷酸序列作为A血清型FMD的VP1区，和SEQ ID NO：2的核苷酸序列作为O血清型FMD的VP1区，制备一种抗A和O血清型FMD的二价DNA疫苗，将其命名为“PLS-FMD-A,O”。
将具有PLS-FMD-A,O的大肠杆菌接种至2.5L的LB培养基中，在37℃下过夜培养。然后使用EndoFree Plasmid Giga试剂盒(QIAGEN,Cat#12391)从培养的大肠杆菌中提取质粒。将提取的质粒溶解在无内毒素水中并储存在-70℃下。然后将储存的质粒用于抗体形成实验，以确认动物的疫苗接种效果。
实施例5.新型DNA二价疫苗的抗体形成效应
5.1 DNA疫苗接种
将15只C57BL/6小鼠(8周龄，雌性)分成三组，每组5只，每组每隔两周接种3次质粒。第2组接种A血清型和O血清型的单价疫苗的组合，第3组接种A血清型和O血清型的二价疫苗。使用电穿孔装置(cellectra 2000)进行接种，并以30μg/小鼠的浓度施用DNA疫苗。
·第1组(对照组)-模拟质粒DNA
·第2组(实验组)-A血清型FMD质粒+PLS-FMD-O
·第3组(实验组)-PLS-FMD-A,O
在给药前(第0周)、第二次给药后(第4周)和第三次给药后(第6周)使用眼眶后采血法采集血液。从收集的血液中分离血清并储存在-80℃。将储存的血清溶解并进行SPC(固相竞争)ELISA分析。
5.2测量抗体形成-SPC ELISA分析
根据试剂盒制造商提供的实验方案，使用PrioCHECK A血清型FMDV ELISA试剂盒(Prionics lelystad B.V/the Netherlands)进行SPC ELISA。
结果如图7所示。图7中可见，即使与其他血清型的FMD疫苗(例如A型)同时接种，PLS-FMD-O也表现出显着高水平的PI值。此外，单独接种二价疫苗也表现出显着高水平的PI值。
具体地，这表示O血清型抗体和A血清型抗体都在接种后的早期阶段形成。此外，可以确认的是，根据本发明的重组O型疫苗即使与其他血清型的疫苗组合接种或以二价疫苗的形式接种，也不会抵消不同血清型之间的影响。因此，根据本发明的重组O血清型疫苗已被证明既可作为单价疫苗也可作为多价疫苗。
实施例6.新型DNA三价疫苗的制备
使用先前制备的重组序列制备针对A、O和Asia1血清型FMD的三价DNA疫苗。
图8显示了制备三价疫苗的示例。在三价疫苗中，A血清型FMD的VP1区可以包含SEQID NO：88、89或90的核苷酸序列，O血清型FMD的VP1区可以包含SEQ ID NO：2的核苷酸序列，Asia1血清型FMD的VP1区可以包含常规已知的核苷酸序列。
使用SEQ ID NO：89的核苷酸序列作为A血清型FMD的VP1区，SEQ ID NO：2的核苷酸序列作为O血清型FMD的VP1区，以及，SEQ ID NO：93的核苷酸序列作为Asia1血清型FMD的VP1区，制备针对FMD的A、O和Asia1血清型的三价DNA疫苗，将其命名为“PLS-FMD-A,O,Asia1”。作为Asia1血清型FMD的VP1区的SEQ ID NO：93的核苷酸序列是美国INOVIO提供的pGX9010中包含的Asia1血清型FMD的VP1区。
以与实施例4相同的方式制备PLS-FMD-A,O,Asia1质粒。
实施例7.新型DNA三价疫苗的抗体形成效果
7.1.DNA疫苗接种
实施例7的制备方法与实施例5相同，但第1组至第3组设置如下：
·第1组(对照组)-模拟质粒DNA
·第2组(实验组)-A血清型FMD质粒+PLS-FMD-O+Asia1血清型FMD质粒
·第3组(实验组)-PLS-FMD-A,O,Asia1
7.2.测量抗体形成-SPC ELISA分析
进行SPC ELISA分析，如图9所示，PLS-FMD-O即使与其他血清型如A和Asia1型的FMD疫苗共同接种时也表现出显着高水平的PI值。此外，单独接种三价疫苗也表现出显着高水平的PI值。
因此，根据本发明的重组O血清型疫苗已被证明既可作为单价疫苗又可作为多价疫苗。
序列表
<110> 株式会社准绳生命科学
<120> 口蹄疫病毒疫苗组合物
<130> 24255KSR
<150> KR 10-2019-0077833
<151> 2019-06-28
<150> KR 10-2019-0077839
<151> 2019-06-28
<160> 95
<170> SIPOSequenceListing 1.0
<210> 1
<211> 213
<212> PRT
<213> Artificial Sequence
<220>
<223> FMDV VP1
<400> 1
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 2
<211> 639
<212> DNA
<213> Artificial Sequence
<220>
<223> FMD type O
<400> 2
accacctcca ccggagagtc cgccgaccca gtgaccacca ccgtggagaa ctacggcggc 60
gagacacagg tgcagcggcg ccagcacacc gacgtgagct tcatcctgga ccggttcgtg 120
aaggtgaccc caaaggacca gatcaacgtg ctggacctga tgcagacccc tgcacacacc 180
ctggtgggcg ccctgctgag gaccgccacc tactacttcg ccgacctgga ggtggccgtg 240
aagcacgagg gcaacctgac ctgggtgcct aacggagcac cagaggccgc cctggacaac 300
accaccaacc caaccgcata ccacaaggcc cctctgacca ggctggccct gccatacacc 360
gcccctcaca gggtgctggc aaccgtgtac aacggcaact gcaagtacgg agagggcgct 420
gtgaccaacg tgcgcggcga cctgcaggtg ctggcacaga aggcagcaag gaccctgccc 480
accagcttca actacggcgc catcaaggcc accagggtga ccgagctgct gtacagaatg 540
aagcgggccg agacatactg tcccagacca ctgctggcaa tccacccttc ccaggcaagg 600
cacaagcaga agatcgtggc accagtgaag cagctgctg 639
<210> 3
<211> 803
<212> DNA
<213> Artificial Sequence
<220>
<223> PLS-FMD-O
<400> 3
tggccagcgc cggcaaggac ttcgacctga gactgccagt ggacgcaagg acccagaggg 60
gaagaaagag gagaagcacc acctccaccg gagagtccgc cgacccagtg accaccaccg 120
tggagaacta cggcggcgag acacaggtgc agcggcgcca gcacaccgac gtgagcttca 180
tcctggaccg gttcgtgaag gtgaccccaa aggaccagat caacgtgctg gacctgatgc 240
agacccctgc acacaccctg gtgggcgccc tgctgaggac cgccacctac tacttcgccg 300
acctggaggt ggccgtgaag cacgagggca acctgacctg ggtgcctaac ggagcaccag 360
aggccgccct ggacaacacc accaacccaa ccgcatacca caaggcccct ctgaccaggc 420
tggccctgcc atacaccgcc cctcacaggg tgctggcaac cgtgtacaac ggcaactgca 480
agtacggaga gggcgctgtg accaacgtgc gcggcgacct gcaggtgctg gcacagaagg 540
cagcaaggac cctgcccacc agcttcaact acggcgccat caaggccacc agggtgaccg 600
agctgctgta cagaatgaag cgggccgaga catactgtcc cagaccactg ctggcaatcc 660
acccttccca ggcaaggcac aagcagaaga tcgtggcacc agtgaagcag ctgctgaggg 720
gacgcaagag gcgctcaacc ctgaacttcg acctcctcaa actggctggc gatgtggaaa 780
gcaatcctgg atgataactc gag 803
<210> 4
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 4
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ser His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Ala Gly Gly Ser Leu Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Pro Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Ala Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 5
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 5
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 6
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 6
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 7
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 7
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Thr Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 8
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 8
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 9
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 9
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 10
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 10
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 11
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 11
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Val Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Asn Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Ala Asn Asn Val
130 135 140
Arg Gly Asp Leu His Val Leu Ala Lys Asn Ala Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Ala Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Asn Thr Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 12
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 12
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Val
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 13
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 13
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 14
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 14
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Ala Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 15
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 15
Thr Thr Ser Thr Gly Glu Thr Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Val
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Ile Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ala Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 16
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 16
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Val Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 17
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 17
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Lys Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Ala Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Gly Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 18
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 18
Thr Thr Ser Thr Gly Glu Thr Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Val
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Ile Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ala Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 19
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 19
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 20
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 20
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Gln Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 21
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 21
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Ala Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Gln Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 22
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 22
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 23
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 23
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Glu Asn Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 24
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 24
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Glu Asn Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 25
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 25
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Glu Asn Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 26
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 26
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 27
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 27
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 28
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 28
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 29
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 29
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 30
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 30
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 31
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 31
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Ala Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Val Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Val Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Cys Leu
210
<210> 32
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 32
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Ala Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Val Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Val Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 33
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 33
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 34
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 34
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Val
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 35
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 35
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 36
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 36
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Ile
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Glu Asp Glu Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Val Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Thr Ser Thr Asn Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Arg Lys Thr Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Phe Gly Ala Ile Lys Ala Ala Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Ser Ala Arg His Lys Gln Arg Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 37
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 37
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Pro His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Ala Gly Gly Ser Leu Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 38
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 38
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Ala Pro Gly Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asp Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 39
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 39
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Pro His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Lys Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Ala Gly Gly Ser Leu Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 40
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 40
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Ala Pro Pro His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Ala Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Tyr Lys Tyr Ala Gly Gly Ser Leu Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 41
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 41
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ser His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Ala Gly Gly Ser Leu Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 42
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 42
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ser His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Ala Gly Gly Ser Leu Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 43
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 43
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Ala Pro Pro His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Ala Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Tyr Lys Tyr Ala Gly Gly Ser Leu Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 44
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 44
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Ser Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Pro His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Ala Gly Gly Ser Leu Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Ser Ala Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Ser Leu
210
<210> 45
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 45
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asp Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 46
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 46
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 47
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 47
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Thr Gln Arg Arg Gln His Thr Asp Ile
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Lys Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Thr Asn Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Thr Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Phe Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Asp Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 48
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 48
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Thr Gln Arg Arg Gln His Thr Asp Ile
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Lys Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Ala Asn Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Thr Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Phe Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Asp Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Thr Lys Gln Leu Leu
210
<210> 49
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 49
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Thr Gln Arg Arg Gln His Thr Asp Ile
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Lys Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Thr Asn Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Thr Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Phe Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Asp Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 50
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 50
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Ile
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Glu Asp Glu Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Val Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Thr Ser Thr Asn Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Arg Lys Thr Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Phe Gly Ala Ile Lys Ala Ala Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Ser Ala Arg His Lys Gln Arg Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 51
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 51
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Asn Thr Asn Val
130 135 140
Arg Gly Asp Leu Arg Glu Leu Ala Gln Lys Thr Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Thr Ala Arg His Lys Gln Arg Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 52
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 52
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Asn Thr Asn Val
130 135 140
Arg Gly Asp Leu Arg Glu Leu Ala Gln Lys Ala Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Thr Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 53
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 53
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Asn Thr Asn Val
130 135 140
Arg Gly Asp Leu Arg Glu Leu Ala Gln Lys Ala Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Thr Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 54
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 54
Thr Thr Ser Val Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Val Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asn Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Asn Thr Asp Val
130 135 140
Arg Gly Asp Leu Arg Glu Leu Ala Gln Lys Val Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Thr Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 55
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 55
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 56
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 56
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Ser Asn Val Pro Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 57
<211> 211
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 57
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Gln Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln
210
<210> 58
<211> 211
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 58
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Gln Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln
210
<210> 59
<211> 211
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 59
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Gln Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln
210
<210> 60
<211> 211
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 60
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Arg Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Gln Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln
210
<210> 61
<211> 211
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 61
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Arg Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Gln Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln
210
<210> 62
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 62
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu His Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Ala Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Asn Lys Tyr Gly Asp Ala Ser Ala Asn Asn Ile
130 135 140
Arg Gly Asp Leu His Val Leu Ala Lys Asn Ala Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Ala Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Thr Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 63
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 63
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu Gln Val
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Ser Lys Tyr Gly Asp Ala Ser Ala Asn Asn Val
130 135 140
Arg Gly Asp Leu His Val Leu Ala Lys Asn Ala Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Val Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile Gln Pro Ser Thr Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 64
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 64
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 65
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 65
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 66
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 66
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Ala Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ala Phe Ile Leu Asp Arg Phe Val Lys Val Lys Pro Gln Glu Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Ile Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Leu Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Glu Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Ser Asn Lys Tyr Gly Asp Thr Ser Ala Asn Asn Val
130 135 140
Arg Gly Asp Leu His Val Leu Ala Lys Asn Ala Glu Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Thr Gln Pro Ser Thr Asp Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Ala Lys Gln Leu Leu
210
<210> 67
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 67
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 68
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 68
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 69
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 69
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 70
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 70
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 71
<211> 211
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 71
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln
210
<210> 72
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 72
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 73
<211> 211
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 73
Thr Thr Ser Ala Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ser His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Glu Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Ala Glu Gly Ser Leu Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Pro Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Val His Pro Asp Gln Ser Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln
210
<210> 74
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 74
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 75
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 75
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 76
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 76
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Ala Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Arg Tyr Gly Ala Ser Pro Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Ser Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 77
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 77
Thr Thr Ser Thr Gly Glu Tyr Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 78
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 78
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 79
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 79
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 80
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 80
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 81
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 81
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Leu Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 82
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 82
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Leu Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 83
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 83
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Thr
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 84
<211> 213
<212> PRT
<213> Unknown
<220>
<223> FMDV VP1
<400> 84
Thr Thr Ser Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Val Gln Arg Arg Gln His Thr Asp Val
20 25 30
Ser Phe Ile Leu Asp Arg Phe Val Lys Val Thr Pro Lys Asp Gln Ile
35 40 45
Asn Val Leu Asp Leu Met Gln Thr Pro Ala His Thr Leu Val Gly Ala
50 55 60
Leu Leu Arg Thr Ala Thr Tyr Tyr Phe Ala Asp Leu Glu Val Ala Val
65 70 75 80
Lys His Glu Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Asn Cys Lys Tyr Gly Glu Gly Ala Val Thr Asn Val
130 135 140
Arg Gly Asp Leu Gln Val Leu Ala Gln Lys Ala Ala Arg Thr Leu Pro
145 150 155 160
Thr Ser Phe Asn Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu
165 170 175
Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu
180 185 190
Ala Ile His Pro Glu Gln Ala Arg His Lys Gln Lys Ile Val Ala Pro
195 200 205
Val Lys Gln Leu Leu
210
<210> 85
<211> 212
<212> PRT
<213> Artificial Sequence
<220>
<223> FMDV A VP1
<400> 85
Thr Thr Ala Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Ala Gln Arg Arg His His Thr Asp Val
20 25 30
Gly Phe Leu Met Asp Arg Phe Val Gln Ile Lys Pro Val Ser Pro Thr
35 40 45
His Val Ile Asp Leu Met Gln Thr His Gln His Gly Leu Val Gly Ala
50 55 60
Met Leu Arg Ala Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Val Val
65 70 75 80
Asn His Thr Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asn Asn Thr Ser Asn Pro Thr Ala Tyr His Lys Ala Pro Phe
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Ser Gly Thr Ser Lys Tyr Ser Thr Pro Gln Thr Arg Arg Gly
130 135 140
Asp Leu Gly Pro Leu Ala Ala Arg Leu Ala Ala Gln Leu Pro Ala Ser
145 150 155 160
Phe Asn Phe Gly Ala Ile Arg Ala Thr Glu Ile Gln Glu Leu Leu Val
165 170 175
Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu Ala Val
180 185 190
Glu Val Ser Ser Gln Asp Arg His Lys Gln Lys Ile Ile Ala Pro Ala
195 200 205
Lys Gln Leu Leu
210
<210> 86
<211> 212
<212> PRT
<213> Artificial Sequence
<220>
<223> FMDV A VP1
<400> 86
Thr Thr Ala Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu
1 5 10 15
Asn Tyr Gly Gly Glu Thr Gln Ala Gln Arg Arg His His Thr Asp Val
20 25 30
Ser Phe Ile Met Asp Arg Phe Val Arg Ile Lys Pro Val Ser Pro Thr
35 40 45
His Val Ile Asp Leu Met Gln Thr His Gln His Gly Leu Val Gly Ala
50 55 60
Met Leu Arg Ala Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Val Val
65 70 75 80
Asn His Thr Gly Asn Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Ala
85 90 95
Ala Leu Asp Asn Thr Ser Asn Pro Thr Ala Tyr His Lys Ala Pro Phe
100 105 110
Thr Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr
115 120 125
Val Tyr Asn Gly Thr Ser Lys Tyr Ser Thr Pro Gly Ala Arg Arg Gly
130 135 140
Asp Leu Gly Ser Leu Ala Ala Arg Leu Ala Ala Gln Leu Pro Ala Ser
145 150 155 160
Phe Asn Phe Gly Ala Ile Arg Ala Thr Glu Ile Gln Glu Leu Leu Val
165 170 175
Arg Met Lys Arg Ala Glu Leu Tyr Cys Pro Arg Pro Leu Leu Ala Val
180 185 190
Glu Val Leu Ser Gln Asp Arg His Lys Gln Lys Ile Ile Ala Pro Ala
195 200 205
Lys Gln Leu Leu
210
<210> 87
<211> 446
<212> PRT
<213> Artificial Sequence
<220>
<223> FMDV A VP4-VP2-VP3
<400> 87
Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu Asp Arg Ile Leu Thr
1 5 10 15
Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln Ser Ser Val Gly Val
20 25 30
Thr His Gly Tyr Ser Thr Glu Glu Asp His Val Ala Gly Pro Asn Thr
35 40 45
Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu Arg Phe Tyr Lys Lys
50 55 60
Tyr Leu Phe Asp Trp Thr Thr Asp Lys Ala Phe Gly His Leu Glu Lys
65 70 75 80
Leu Glu Leu Pro Ser Asp His His Gly Val Phe Gly His Leu Val Asp
85 90 95
Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val Glu Val Ser Ala Val
100 105 110
Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val Ala Met Val Pro Glu
115 120 125
Trp Lys Glu Phe Asp Thr Arg Glu Lys Tyr Gln Leu Thr Leu Phe Pro
130 135 140
His Gln Phe Ile Ser Pro Arg Thr Asn Met Thr Ala His Ile Thr Val
145 150 155 160
Pro Tyr Leu Gly Val Asn Arg Tyr Asp Gln Tyr Lys Lys His Lys Pro
165 170 175
Trp Thr Leu Val Val Met Val Val Ser Pro Leu Thr Val Asn Asn Thr
180 185 190
Ser Ala Ala Gln Ile Lys Val Tyr Ala Asn Ile Ala Pro Thr Tyr Val
195 200 205
His Val Ala Gly Glu Leu Pro Ser Lys Glu Arg Gly Arg Lys Arg Arg
210 215 220
Ser Gly Ile Phe Pro Val Ala Cys Ala Asp Gly Tyr Gly Gly Leu Val
225 230 235 240
Thr Thr Asp Pro Lys Thr Ala Asp Pro Ala Tyr Gly Lys Val Tyr Asn
245 250 255
Pro Pro Arg Thr Asn Tyr Pro Gly Arg Phe Thr Asn Leu Leu Asp Val
260 265 270
Ala Glu Ala Cys Pro Thr Phe Leu Cys Phe Asp Asp Gly Lys Pro Tyr
275 280 285
Val Thr Thr Arg Thr Asp Asp Thr Arg Leu Leu Ala Lys Phe Asp Leu
290 295 300
Ser Leu Ala Ala Lys His Met Ser Asn Thr Tyr Leu Ser Gly Ile Ala
305 310 315 320
Gln Tyr Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Leu His Phe Met Phe
325 330 335
Thr Gly Ser Thr Asp Ser Lys Ala Arg Tyr Met Val Ala Tyr Ile Pro
340 345 350
Pro Gly Val Glu Thr Pro Pro Asp Thr Pro Glu Arg Ala Ala His Cys
355 360 365
Ile His Ala Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser
370 375 380
Ile Pro Tyr Val Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Thr
385 390 395 400
Ala Glu Thr Ile Asn Val Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr
405 410 415
His Gly Lys Ala Glu Asn Asp Thr Leu Val Val Ser Val Ser Ala Gly
420 425 430
Lys Asp Phe Glu Leu Arg Leu Pro Ile Asp Pro Arg Gln Gln
435 440 445
<210> 88
<211> 636
<212> DNA
<213> Artificial Sequence
<220>
<223> FMDV A VP1
<400> 88
accaccgcca ccggggaatc agcagaccct gtcacaacca ccgttgagaa ctacggtggc 60
gagacacaag cacagcggcg tcaccacacc gacgtcggct tcttaatgga caggttcgtg 120
cagatcaagc ctgtgagccc cacacatgtc attgacctca tgcagacaca ccaacacggg 180
ctggtgggcg ccatgttgcg cgcggccacc tactactttt ctgatcttga gattgtggtg 240
aaccacacgg gtaacctaac gtgggtaccc aatggagcac ccgaggcagc actgaacaac 300
acgagcaacc ccactgctta ccacaaagcg ccgttcacga ggcttgcgct cccctacacc 360
gcgccacacc gcgtgctggc aactgtgtac agcgggacga gcaagtactc cacacctcaa 420
acacggcgag gtgacctggg tcctctcgcg gcgaggctcg ctgcacagct ccctgcctcc 480
ttcaacttcg gtgcaattcg ggccacggag atccaagaac tccttgtgcg catgaagcgc 540
gccgagctct actgccccag gccactgttg gcggtggagg tgtcgtcgca agacagacac 600
aagcagaaaa tcattgcccc tgcaaaacaa ctcctg 636
<210> 89
<211> 636
<212> DNA
<213> Artificial Sequence
<220>
<223> FMDV A VP1 DNA porcine codon optimization
<400> 89
acaacagcca caggagaatc cgccgaccca gtgacaacca ccgtggagaa ctacggagga 60
gagacacagg ctcagcgccg gcaccacacc gacgtgggct tcctgatgga caggttcgtg 120
cagatcaagc ccgtgagccc aacccacgtg atcgacctga tgcagaccca ccagcacggc 180
ctggtgggag ctatgctgag ggctgccacc tactacttct ccgacctgga gatcgtggtg 240
aaccacaccg gaaacctgac ctgggtgcca aacggagctc cagaggccgc cctgaacaac 300
accagcaacc ccaccgccta ccacaaggct ccattcacca ggctggccct gccatacacc 360
gctccacacc gggtgctggc taccgtgtac tccggcacca gcaagtactc caccccacag 420
accaggagag gcgacctggg accactggct gctaggctgg ctgctcagct gccagcctcc 480
ttcaacttcg gagctatcag ggctaccgag atccaggagc tgctggtgag gatgaagaga 540
gccgagctgt actgccccag gccactgctg gctgtggagg tgtccagcca ggacagacac 600
aagcagaaga tcatcgcccc agccaagcag ctgctg 636
<210> 90
<211> 636
<212> DNA
<213> Artificial Sequence
<220>
<223> FMDV A VP1
<400> 90
acaacagcca caggagaatc cgccgaccca gtgacaacca ccgtggagaa ctacggagga 60
gagacacagg ctcagcgccg gcaccacacc gacgtgggct tcctgatgga caggttcgtg 120
cagatcaagc ccgtgagccc aacccacgtg atcgacctga tgcagaccca ccagcacggc 180
ctggtgggag ctatgctgag ggctgccacc tactacttct ccgacctgga gatcgtggtg 240
aaccacaccg gaaacctgac ctgggtgcca aacggagctc cagaggccgc cctgaacaac 300
accagcaacc ccaccgccta ccacaaggct ccattcacca ggctggccct gccatacacc 360
gctccacacc gggtgctggc taccgtgtac tccggcacca gcaagtactc caccccacag 420
accaggagag gcgacctggg accactggct gctaggctgg ctgctcagct gccagcctcc 480
ttcaacttcg gagctatcag ggctaccgag atccaggagc tgctggtgag gatgaagaga 540
gccgagctgt actgccccag gccactgctg gctgtggagg tgtccagcca ggacagacac 600
aagcagaaga tcatcgcccc agccaagcag ctgctg 636
<210> 91
<211> 636
<212> DNA
<213> Artificial Sequence
<220>
<223> FMDV A VP4-VP2-VP3
<400> 91
acaacagcca caggagaatc cgccgaccca gtgacaacca ccgtggagaa ctacggagga 60
gagacacagg ctcagcgccg gcaccacacc gacgtgggct tcctgatgga caggttcgtg 120
cagatcaagc ccgtgagccc aacccacgtg atcgacctga tgcagaccca ccagcacggc 180
ctggtgggag ctatgctgag ggctgccacc tactacttct ccgacctgga gatcgtggtg 240
aaccacaccg gaaacctgac ctgggtgcca aacggagctc cagaggccgc cctgaacaac 300
accagcaacc ccaccgccta ccacaaggct ccattcacca ggctggccct gccatacacc 360
gctccacacc gggtgctggc taccgtgtac tccggcacca gcaagtactc caccccacag 420
accaggagag gcgacctggg accactggct gctaggctgg ctgctcagct gccagcctcc 480
ttcaacttcg gagctatcag ggctaccgag atccaggagc tgctggtgag gatgaagaga 540
gccgagctgt actgccccag gccactgctg gctgtggagg tgtccagcca ggacagacac 600
aagcagaaga tcatcgcccc agccaagcag ctgctg 636
<210> 92
<211> 2400
<212> DNA
<213> Artificial Sequence
<220>
<223> FMDV A VP4-VP2-VP3-VP1
<400> 92
gccaccatgg attggacatg gattctgttc ctggtggctg ctgctactag agtgcattca 60
ggggccggac agtcttcacc cgcaaccgga tcacagaacc agagtggaaa taccgggagc 120
atcattaaca attactatat gcagcagtac cagaacagca tggacacaca gctgggggat 180
aacgccatca gcggcggcag caatgagggc tccacagata ccacatctac tcacactacc 240
aatacccaga acaatgactg gttctctaaa ctggcaagct ccgccttcac cggcctcttt 300
ggagctctgc tcgcaagggg aagaaagagg agaagcgata agaaaacaga ggaaaccacc 360
ctgctggagg acagaatcct gaccacaaga aacgggcaca ctaccagcac aactcagtct 420
tcagtgggcg tcacacacgg atactcaact gaggaagacc atgtggccgg gccaaatacc 480
agtggcctgg agacacgagt ggtccaggct gaaaggttct acaagaaata tctgtttgac 540
tggaccacag ataaggcctt cggccacctg gagaaactgg aactcccctc agaccaccac 600
ggcgtgttcg gccatctggt cgatagctac gcctatatga gaaacggatg ggacgtggag 660
gtctccgctg tgggcaacca gttcaatggc ggatgcctgc tcgtggctat ggtgcccgag 720
tggaaggaat ttgataccag ggaaaaatac cagctgacac tcttcccaca ccagtttatc 780
tctcctagaa ctaacatgac cgcccatatt accgtgcctt atctgggcgt caatcggtac 840
gaccagtata agaaacacaa accttggacc ctggtggtca tggtggtcag tcccctcaca 900
gtgaacaata ctagcgccgc tcagatcaag gtctacgcca acattgctcc aacctatgtg 960
cacgtcgcag gagagctgcc ttccaaggaa cggggacgca aacggcgctc tgggatcttc 1020
ccagtggcat gtgctgacgg atacggaggg ctggtcacta ccgaccctaa gaccgcagat 1080
cccgcctacg gaaaagtgta taacccaccc aggactaatt acccagggcg gttcaccaac 1140
ctgctcgatg tggcagaggc ctgccccacc ttcctgtgct ttgacgatgg caagccatac 1200
gtgacaactc ggacagacga tactcgcctg ctcgccaagt ttgacctgag cctcgcagcc 1260
aaacacatgt caaacaccta cctgagtgga atcgcccagt actatactca gtattccggg 1320
accattaatc tgcatttcat gtttaccggc tctacagact caaaggctcg ctacatggtg 1380
gcatatatcc ctcccggcgt cgagacccca cctgatacac ctgaaagggc tgcacactgc 1440
atccatgccg agtgggacac aggactgaac agcaagttca ctttttccat tccctacgtg 1500
tctgccgctg actacgctta taccgcatcc gatactgccg aaaccattaa cgtgcaggga 1560
tgggtctgta tctaccagat tactcacggg aaagccgaga atgacaccct ggtggtctcc 1620
gtgtctgctg gcaaggactt cgaactcaga ctccccattg acccaagaca gcagagaggc 1680
agaaaaagac gcagcacaac agccacagga gaatccgccg acccagtgac aaccaccgtg 1740
gagaactacg gaggagagac acaggctcag cgccggcacc acaccgacgt gggcttcctg 1800
atggacaggt tcgtgcagat caagcccgtg agcccaaccc acgtgatcga cctgatgcag 1860
acccaccagc acggcctggt gggagctatg ctgagggctg ccacctacta cttctccgac 1920
ctggagatcg tggtgaacca caccggaaac ctgacctggg tgccaaacgg agctccagag 1980
gccgccctga acaacaccag caaccccacc gcctaccaca aggctccatt caccaggctg 2040
gccctgccat acaccgctcc acaccgggtg ctggctaccg tgtactccgg caccagcaag 2100
tactccaccc cacagaccag gagaggcgac ctgggaccac tggctgctag gctggctgct 2160
cagctgccag cctccttcaa cttcggagct atcagggcta ccgagatcca ggagctgctg 2220
gtgaggatga agagagccga gctgtactgc cccaggccac tgctggctgt ggaggtgtcc 2280
agccaggaca gacacaagca gaagatcatc gccccagcca agcagctgct gcgcggccgg 2340
aaacgacgct ctaattttga cctcctgaaa ctggctggcg atgtggaatc taaccctggc 2400
<210> 93
<211> 633
<212> DNA
<213> Artificial Sequence
<220>
<223> FMDV Asia1 type VP1
<400> 93
actaccacaa ctggggaatc agccgaccca gtcaccacaa ctgtggagaa ctacggcgga 60
gaaacccaga cagcaagacg gctgcacacc gacgtggcct tcatcctcga tcgctttgtg 120
aagctgacag cccccaaaaa tatccagact ctggacctca tgcagattcc atcccataca 180
ctggtgggcg cactgctcag gagtgccact tactatttca gcgacctgga ggtcgctctc 240
gtgcacactg gaccagtcac ctgggtgcct aacggagcac caaaggatgc tctgaacaat 300
cagaccaatc caacagccta ccagaaacag cctatcacca ggctggctct cccatataca 360
gcacctcaca gagtcctggc taccgtgtac aacggaaaga ccgcctacgg cgagaccaca 420
agccgccgag gcgacatggc agccctggcc cagcggctct ccgctcgcct gcccacatct 480
ttcaattacg gagcagtgaa ggccgatact atcaccgagc tgctcattag gatgaaaaga 540
gccgaaacct attgccccag gccactgctc gctctggaca ctacccagga taggagaaag 600
caggagatca ttgccccaga aaaacaggtg ctg 633
<210> 94
<211> 2262
<212> DNA
<213> Artificial Sequence
<220>
<223> FMDV Asia1 type VP4-VP2-VP3-VP1
<400> 94
ggggccggac agtcttcacc tgctactggg agccagaacc agagcggaaa tacagggtcc 60
atcattaaca attactatat gcagcagtac cagaacagca tggacaccca gctgggcgat 120
aacgccatct ccggcggatc taatgaggga tctactgaca ccacatcaac acacactaac 180
aatacccaga acaatgattg gttcagtaga ctcgccagct ccgctttctc tggactgttt 240
ggagcactgc tcgcccgggg ccgcaagagg agatccgaca agaaaaccga ggaaaccacc 300
ctgctggagg atcgaatcct gacaactagg aacggacata ccacaagcac tacccagtct 360
tcagtgggag tcacctacgg gtatgctgtc gcagaagacg ccgtgagtgg gcccaacaca 420
agcggcctgg agactagagt gcagcaggct gaacggttct ttaagaaaca cctcttcgat 480
tggacaccta atctggcctt tggccattgc tactatctgg agctccccac cgaacacaag 540
ggggtgtacg gctcactgat ggggagttac gcatatatgc ggaacggatg ggacatcgag 600
gtgaccgcag tcggaaacca gttcaatggc ggatgtctgc tcgtggctct ggtccctgag 660
ctgaaggaac tcgatacaag gcagaaatac cagctgactc tcttccctca tcagtttatt 720
aaccccagaa caaatatgac tgcccacatc aacgtgccct acgtcggcat taatcggtac 780
gaccagtatg ccctccataa gccttggacc ctggtggtca tggtggtcgc tcccctgacc 840
gtgaagacag gagggtccga gcagatcaaa gtgtacatga acgccgctcc aacctatgtg 900
cacgtcgccg gcgagctgcc ttcaaaggaa cgaggcagga aacggcgctc tggaattgtg 960
ccagtcgcat gcgctgacgg atacggaaac atggtgacaa ctgaccccaa gaccgccgat 1020
ccagtctatg gaaaagtgtt caacccaccc aggaccaatc tccctgggcg attcacaaac 1080
tttctggatg tggcagaggc ctgtcccaca ttcctgcggt ttggggaagt gccattcgtc 1140
aagaccgtga acagcggcga ccgactgctc gccaaatttg acgtgagcct ggccgccggc 1200
cacatgagta acacctacct ggctggactc gcacagtact atacccagta tagcgggaca 1260
atgaatgtgc acttcatgtt tactggccca accgacgcta aggcaagata catggtcgcc 1320
tatgtgcctc ccgggatgac accacctact gaccctgagc acgctgcaca ttgcatccac 1380
agcgaatggg atactggcct caactccaaa ttcacctttt ctattcccta cctgtcagcc 1440
gctgactacg cctatacagc cagcgatgtg gccgagacca catccgtcca gggatgggtg 1500
tgcatctacc agattaccca cggcaaggct gagggagacg cactggtggt gagcgtgagc 1560
gccgggaaag acttcgaatt tcggctgccc gtggatgcac gccagcagag aggacggaag 1620
cgaaggtcta ctaccacaac tggggaatca gccgacccag tcaccacaac tgtggagaac 1680
tacggcggag aaacccagac agcaagacgg ctgcacaccg acgtggcctt catcctcgat 1740
cgctttgtga agctgacagc ccccaaaaat atccagactc tggacctcat gcagattcca 1800
tcccatacac tggtgggcgc actgctcagg agtgccactt actatttcag cgacctggag 1860
gtcgctctcg tgcacactgg accagtcacc tgggtgccta acggagcacc aaaggatgct 1920
ctgaacaatc agaccaatcc aacagcctac cagaaacagc ctatcaccag gctggctctc 1980
ccatatacag cacctcacag agtcctggct accgtgtaca acggaaagac cgcctacggc 2040
gagaccacaa gccgccgagg cgacatggca gccctggccc agcggctctc cgctcgcctg 2100
cccacatctt tcaattacgg agcagtgaag gccgatacta tcaccgagct gctcattagg 2160
atgaaaagag ccgaaaccta ttgccccagg ccactgctcg ctctggacac tacccaggat 2220
aggagaaagc aggagatcat tgccccagaa aaacaggtgc tg 2262
<210> 95
<211> 754
<212> PRT
<213> Artificial Sequence
<220>
<223> FMDV Asia1 type VP4-VP2-VP3-VP1
<400> 95
Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly
1 5 10 15
Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn
20 25 30
Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn
35 40 45
Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Asn Asn Thr Gln Asn
50 55 60
Asn Asp Trp Phe Ser Arg Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe
65 70 75 80
Gly Ala Leu Leu Ala Arg Gly Arg Lys Arg Arg Ser Asp Lys Lys Thr
85 90 95
Glu Glu Thr Thr Leu Leu Glu Asp Arg Ile Leu Thr Thr Arg Asn Gly
100 105 110
His Thr Thr Ser Thr Thr Gln Ser Ser Val Gly Val Thr Tyr Gly Tyr
115 120 125
Ala Val Ala Glu Asp Ala Val Ser Gly Pro Asn Thr Ser Gly Leu Glu
130 135 140
Thr Arg Val Gln Gln Ala Glu Arg Phe Phe Lys Lys His Leu Phe Asp
145 150 155 160
Trp Thr Pro Asn Leu Ala Phe Gly His Cys Tyr Tyr Leu Glu Leu Pro
165 170 175
Thr Glu His Lys Gly Val Tyr Gly Ser Leu Met Gly Ser Tyr Ala Tyr
180 185 190
Met Arg Asn Gly Trp Asp Ile Glu Val Thr Ala Val Gly Asn Gln Phe
195 200 205
Asn Gly Gly Cys Leu Leu Val Ala Leu Val Pro Glu Leu Lys Glu Leu
210 215 220
Asp Thr Arg Gln Lys Tyr Gln Leu Thr Leu Phe Pro His Gln Phe Ile
225 230 235 240
Asn Pro Arg Thr Asn Met Thr Ala His Ile Asn Val Pro Tyr Val Gly
245 250 255
Ile Asn Arg Tyr Asp Gln Tyr Ala Leu His Lys Pro Trp Thr Leu Val
260 265 270
Val Met Val Val Ala Pro Leu Thr Val Lys Thr Gly Gly Ser Glu Gln
275 280 285
Ile Lys Val Tyr Met Asn Ala Ala Pro Thr Tyr Val His Val Ala Gly
290 295 300
Glu Leu Pro Ser Lys Glu Arg Gly Arg Lys Arg Arg Ser Gly Ile Val
305 310 315 320
Pro Val Ala Cys Ala Asp Gly Tyr Gly Asn Met Val Thr Thr Asp Pro
325 330 335
Lys Thr Ala Asp Pro Val Tyr Gly Lys Val Phe Asn Pro Pro Arg Thr
340 345 350
Asn Leu Pro Gly Arg Phe Thr Asn Phe Leu Asp Val Ala Glu Ala Cys
355 360 365
Pro Thr Phe Leu Arg Phe Gly Glu Val Pro Phe Val Lys Thr Val Asn
370 375 380
Ser Gly Asp Arg Leu Leu Ala Lys Phe Asp Val Ser Leu Ala Ala Gly
385 390 395 400
His Met Ser Asn Thr Tyr Leu Ala Gly Leu Ala Gln Tyr Tyr Thr Gln
405 410 415
Tyr Ser Gly Thr Met Asn Val His Phe Met Phe Thr Gly Pro Thr Asp
420 425 430
Ala Lys Ala Arg Tyr Met Val Ala Tyr Val Pro Pro Gly Met Thr Pro
435 440 445
Pro Thr Asp Pro Glu His Ala Ala His Cys Ile His Ser Glu Trp Asp
450 455 460
Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr Leu Ser Ala
465 470 475 480
Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Val Ala Glu Thr Thr Ser Val
485 490 495
Gln Gly Trp Val Cys Ile Tyr Gln Ile Thr His Gly Lys Ala Glu Gly
500 505 510
Asp Ala Leu Val Val Ser Val Ser Ala Gly Lys Asp Phe Glu Phe Arg
515 520 525
Leu Pro Val Asp Ala Arg Gln Gln Arg Gly Arg Lys Arg Arg Ser Thr
530 535 540
Thr Thr Thr Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asn
545 550 555 560
Tyr Gly Gly Glu Thr Gln Thr Ala Arg Arg Leu His Thr Asp Val Ala
565 570 575
Phe Ile Leu Asp Arg Phe Val Lys Leu Thr Ala Pro Lys Asn Ile Gln
580 585 590
Thr Leu Asp Leu Met Gln Ile Pro Ser His Thr Leu Val Gly Ala Leu
595 600 605
Leu Arg Ser Ala Thr Tyr Tyr Phe Ser Asp Leu Glu Val Ala Leu Val
610 615 620
His Thr Gly Pro Val Thr Trp Val Pro Asn Gly Ala Pro Lys Asp Ala
625 630 635 640
Leu Asn Asn Gln Thr Asn Pro Thr Ala Tyr Gln Lys Gln Pro Ile Thr
645 650 655
Arg Leu Ala Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val
660 665 670
Tyr Asn Gly Lys Thr Ala Tyr Gly Glu Thr Thr Ser Arg Arg Gly Asp
675 680 685
Met Ala Ala Leu Ala Gln Arg Leu Ser Ala Arg Leu Pro Thr Ser Phe
690 695 700
Asn Tyr Gly Ala Val Lys Ala Asp Thr Ile Thr Glu Leu Leu Ile Arg
705 710 715 720
Met Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu Ala Leu Asp
725 730 735
Thr Thr Gln Asp Arg Arg Lys Gln Glu Ile Ile Ala Pro Glu Lys Gln
740 745 750
Val Leu
Claims (17)
1.一种多肽，所述多肽包含SEQ ID NO：1的氨基酸序列或包含与SEQ ID NO：1的氨基酸序列具有90％以上同一性的氨基酸序列。
2.一种多肽，所述多肽包含SEQ ID NO：1的氨基酸序列或包含与SEQ ID NO：1的氨基酸序列具有90％以上同一性的氨基酸序列，以及选自口蹄疫病毒的VP2、VP3和VP4蛋白的氨基酸序列中的至少一种。
3.根据权利要求2所述的多肽，其中，所述口蹄疫病毒的VP2、VP3和VP4蛋白来源于O血清型的口蹄疫病毒。
4.一种多核苷酸，所述多核苷酸包含编码SEQ ID NO：1的氨基酸序列或与SEQ ID NO：1的氨基酸序列具有90％以上同一性的氨基酸序列的核苷酸序列，或者SEQ ID NO：2的核苷酸序列或与SEQ ID NO：2的核苷酸序列具有90％以上同一性的核苷酸序列。
5.一种包含权利要求4所述的多核苷酸的质粒。
6.根据权利要求5所述的质粒，所述质粒包含SEQ ID NO：3的核苷酸序列。
7.根据权利要求5所述的质粒，所述质粒还包含编码口蹄疫病毒的VP2、VP3和VP4蛋白中的至少一种的核苷酸序列。
8.根据权利要求7所述的质粒，其中，所述口蹄疫病毒的VP2、VP3和VP4蛋白来自于O血清型口蹄疫病毒。
9.一种用于口蹄疫的疫苗组合物，所述疫苗组合物包含权利要求1-3中任一项所述的多肽、权利要求4所述的多核苷酸或权利要求5-8中任一项所述的质粒。
10.根据权利要求9所述的疫苗组合物，其中，所述疫苗是单价疫苗或多价疫苗。
11.根据权利要求9所述的疫苗组合物，其中，所述多核苷酸还包含口蹄疫病毒的VP1蛋白的氨基酸序列中的至少一种，所述口蹄疫病毒选自A、C、SAT1、SAT2、SAT3和Asia1血清型口蹄疫病毒。
12.根据权利要求9所述的疫苗组合物，其中，所述多核苷酸还包含口蹄疫病毒的VP2、VP3和VP4蛋白的氨基酸序列中的至少一种，所述口蹄疫病毒选自A、C、SAT1、SAT2、SAT3和Asia1血清型口蹄疫病毒。
13.一种在受试者中引发对FMDV的免疫应答的方法，包括：向受试者施用权利要求9所述的疫苗组合物。
14.根据权利要求13所述的方法，其中，所述施用通过电穿孔进行。
15.一种在受试者中预防口蹄疫病毒的方法，包括：向受试者施用权利要求9所述的疫苗组合物。
16.根据权利要求15所述的方法，其中，所述施用通过电穿孔进行。
17.一种用于预防口蹄疫病毒的药物组合物，所述药物组合物包含权利要求1-3中任一项所述的多肽、权利要求4所述的多核苷酸或权利要求5-8中任一项所述的质粒。
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