CN114181908A - 抗人s100b蛋白鼠单克隆抗体及其应用 - Google Patents
抗人s100b蛋白鼠单克隆抗体及其应用 Download PDFInfo
- Publication number
- CN114181908A CN114181908A CN202110828284.3A CN202110828284A CN114181908A CN 114181908 A CN114181908 A CN 114181908A CN 202110828284 A CN202110828284 A CN 202110828284A CN 114181908 A CN114181908 A CN 114181908A
- Authority
- CN
- China
- Prior art keywords
- protein
- monoclonal antibody
- oti6a6
- oti3g6
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101000821885 Homo sapiens Protein S100-B Proteins 0.000 title claims abstract description 38
- 229940126619 mouse monoclonal antibody Drugs 0.000 title claims abstract description 29
- 102000057388 human S100B Human genes 0.000 title claims abstract description 9
- 102000011425 S100 Calcium Binding Protein beta Subunit Human genes 0.000 claims abstract description 47
- 108010023918 S100 Calcium Binding Protein beta Subunit Proteins 0.000 claims abstract description 47
- 102100021487 Protein S100-B Human genes 0.000 claims abstract description 32
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 25
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 22
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 17
- 230000000890 antigenic effect Effects 0.000 claims abstract description 12
- 210000004408 hybridoma Anatomy 0.000 claims abstract description 10
- 238000004321 preservation Methods 0.000 claims abstract description 9
- 238000003118 sandwich ELISA Methods 0.000 claims abstract description 9
- 210000003527 eukaryotic cell Anatomy 0.000 claims abstract description 3
- 230000002163 immunogen Effects 0.000 claims abstract description 3
- 230000003248 secreting effect Effects 0.000 claims abstract 2
- 241001529936 Murinae Species 0.000 claims description 31
- 210000004027 cell Anatomy 0.000 claims description 10
- 238000003018 immunoassay Methods 0.000 claims description 9
- 230000003053 immunization Effects 0.000 claims description 7
- 238000002649 immunization Methods 0.000 claims description 6
- 125000000539 amino acid group Chemical group 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 3
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 3
- 238000009629 microbiological culture Methods 0.000 claims description 2
- 210000000952 spleen Anatomy 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 25
- 238000002360 preparation method Methods 0.000 abstract description 9
- 208000029028 brain injury Diseases 0.000 abstract description 6
- 238000003745 diagnosis Methods 0.000 abstract description 4
- 238000004393 prognosis Methods 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 description 26
- 241001504683 Mus musculus x Rattus norvegicus Species 0.000 description 16
- 239000000243 solution Substances 0.000 description 15
- 102100035360 Cerebellar degeneration-related antigen 1 Human genes 0.000 description 14
- 235000018102 proteins Nutrition 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 13
- 150000001413 amino acids Chemical class 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 12
- 239000000126 substance Substances 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 238000002965 ELISA Methods 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 238000011161 development Methods 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 239000011248 coating agent Substances 0.000 description 6
- 238000000576 coating method Methods 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 210000004989 spleen cell Anatomy 0.000 description 5
- 239000012089 stop solution Substances 0.000 description 5
- 238000001262 western blot Methods 0.000 description 5
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 238000007865 diluting Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 108010051242 phenylalanylserine Proteins 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 101000685725 Homo sapiens Protein S100-A3 Proteins 0.000 description 3
- 101000821881 Homo sapiens Protein S100-P Proteins 0.000 description 3
- 241000880493 Leptailurus serval Species 0.000 description 3
- 102100023090 Protein S100-A3 Human genes 0.000 description 3
- 102100032421 Protein S100-A6 Human genes 0.000 description 3
- 101710122255 Protein S100-B Proteins 0.000 description 3
- 102100021494 Protein S100-P Human genes 0.000 description 3
- 108010005260 S100 Calcium Binding Protein A6 Proteins 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000007910 cell fusion Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000002331 protein detection Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- RCENDENBBJFJHZ-ACZMJKKPSA-N Asn-Asn-Gln Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O RCENDENBBJFJHZ-ACZMJKKPSA-N 0.000 description 2
- DXVMJJNAOVECBA-WHFBIAKZSA-N Asn-Gly-Asn Chemical compound NC(=O)C[C@H](N)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O DXVMJJNAOVECBA-WHFBIAKZSA-N 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- MIIVFRCYJABHTQ-ONGXEEELSA-N Gly-Leu-Val Chemical compound [H]NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(O)=O MIIVFRCYJABHTQ-ONGXEEELSA-N 0.000 description 2
- WNZOCXUOGVYYBJ-CDMKHQONSA-N Gly-Phe-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)CN)O WNZOCXUOGVYYBJ-CDMKHQONSA-N 0.000 description 2
- DNAZKGFYFRGZIH-QWRGUYRKSA-N Gly-Tyr-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CC=C(O)C=C1 DNAZKGFYFRGZIH-QWRGUYRKSA-N 0.000 description 2
- LEDRIAHEWDJRMF-CFMVVWHZSA-N Ile-Asn-Tyr Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 LEDRIAHEWDJRMF-CFMVVWHZSA-N 0.000 description 2
- WCNWGAUZWWSYDG-SVSWQMSJSA-N Ile-Thr-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)O)N WCNWGAUZWWSYDG-SVSWQMSJSA-N 0.000 description 2
- BQSLGJHIAGOZCD-CIUDSAMLSA-N Leu-Ala-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O BQSLGJHIAGOZCD-CIUDSAMLSA-N 0.000 description 2
- PXHCFKXNSBJSTQ-KKUMJFAQSA-N Lys-Asn-Tyr Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCCCN)N)O PXHCFKXNSBJSTQ-KKUMJFAQSA-N 0.000 description 2
- SPSSJSICDYYTQN-HJGDQZAQSA-N Met-Thr-Gln Chemical compound CSCC[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCC(N)=O SPSSJSICDYYTQN-HJGDQZAQSA-N 0.000 description 2
- BPIMVBKDLSBKIJ-FCLVOEFKSA-N Phe-Thr-Phe Chemical compound C([C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 BPIMVBKDLSBKIJ-FCLVOEFKSA-N 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- -1 S100a2 Proteins 0.000 description 2
- MIJWOJAXARLEHA-WDSKDSINSA-N Ser-Gly-Glu Chemical compound OC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCC(O)=O MIJWOJAXARLEHA-WDSKDSINSA-N 0.000 description 2
- UIGMAMGZOJVTDN-WHFBIAKZSA-N Ser-Gly-Ser Chemical compound OC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O UIGMAMGZOJVTDN-WHFBIAKZSA-N 0.000 description 2
- XXXAXOWMBOKTRN-XPUUQOCRSA-N Ser-Gly-Val Chemical compound [H]N[C@@H](CO)C(=O)NCC(=O)N[C@@H](C(C)C)C(O)=O XXXAXOWMBOKTRN-XPUUQOCRSA-N 0.000 description 2
- ZUDXUJSYCCNZQJ-DCAQKATOSA-N Ser-His-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CN=CN1)NC(=O)[C@H](CO)N ZUDXUJSYCCNZQJ-DCAQKATOSA-N 0.000 description 2
- VBMOVTMNHWPZJR-SUSMZKCASA-N Thr-Thr-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(O)=O VBMOVTMNHWPZJR-SUSMZKCASA-N 0.000 description 2
- YOPQYBJJNSIQGZ-JNPHEJMOSA-N Thr-Tyr-Tyr Chemical compound C([C@H](NC(=O)[C@@H](N)[C@H](O)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=C(O)C=C1 YOPQYBJJNSIQGZ-JNPHEJMOSA-N 0.000 description 2
- MNYNCKZAEIAONY-XGEHTFHBSA-N Thr-Val-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O MNYNCKZAEIAONY-XGEHTFHBSA-N 0.000 description 2
- AVYVKJMBNLPWRX-WFBYXXMGSA-N Trp-Ala-Ser Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O)=CNC2=C1 AVYVKJMBNLPWRX-WFBYXXMGSA-N 0.000 description 2
- AGDDLOQMXUQPDY-BZSNNMDCSA-N Tyr-Tyr-Ser Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(O)=O AGDDLOQMXUQPDY-BZSNNMDCSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 108010008685 alanyl-glutamyl-aspartic acid Proteins 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 208000026106 cerebrovascular disease Diseases 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000003113 dilution method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 2
- 108010089804 glycyl-threonine Proteins 0.000 description 2
- 108010050848 glycylleucine Proteins 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 108010060857 isoleucyl-valyl-tyrosine Proteins 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 210000000653 nervous system Anatomy 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 239000012898 sample dilution Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- SVBXIUDNTRTKHE-CIUDSAMLSA-N Ala-Arg-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O SVBXIUDNTRTKHE-CIUDSAMLSA-N 0.000 description 1
- KUDREHRZRIVKHS-UWJYBYFXSA-N Ala-Asp-Tyr Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O KUDREHRZRIVKHS-UWJYBYFXSA-N 0.000 description 1
- KTXKIYXZQFWJKB-VZFHVOOUSA-N Ala-Thr-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(O)=O KTXKIYXZQFWJKB-VZFHVOOUSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241001149231 Arachnis x Vanda Species 0.000 description 1
- DFCIPNHFKOQAME-FXQIFTODSA-N Arg-Ala-Asn Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(O)=O DFCIPNHFKOQAME-FXQIFTODSA-N 0.000 description 1
- VKKYFICVTYKFIO-CIUDSAMLSA-N Arg-Ala-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCCN=C(N)N VKKYFICVTYKFIO-CIUDSAMLSA-N 0.000 description 1
- LLZXKVAAEWBUPB-KKUMJFAQSA-N Arg-Gln-Phe Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O LLZXKVAAEWBUPB-KKUMJFAQSA-N 0.000 description 1
- PRLPSDIHSRITSF-UNQGMJICSA-N Arg-Phe-Thr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PRLPSDIHSRITSF-UNQGMJICSA-N 0.000 description 1
- HUZGPXBILPMCHM-IHRRRGAJSA-N Asn-Arg-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O HUZGPXBILPMCHM-IHRRRGAJSA-N 0.000 description 1
- QPTAGIPWARILES-AVGNSLFASA-N Asn-Gln-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O QPTAGIPWARILES-AVGNSLFASA-N 0.000 description 1
- UDSVWSUXKYXSTR-QWRGUYRKSA-N Asn-Gly-Tyr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O UDSVWSUXKYXSTR-QWRGUYRKSA-N 0.000 description 1
- NCXTYSVDWLAQGZ-ZKWXMUAHSA-N Asn-Ser-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O NCXTYSVDWLAQGZ-ZKWXMUAHSA-N 0.000 description 1
- UPAGTDJAORYMEC-VHWLVUOQSA-N Asn-Trp-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)NC(=O)[C@H](CC(=O)N)N UPAGTDJAORYMEC-VHWLVUOQSA-N 0.000 description 1
- ZVTDYGWRRPMFCL-WFBYXXMGSA-N Asp-Ala-Trp Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CC(=O)O)N ZVTDYGWRRPMFCL-WFBYXXMGSA-N 0.000 description 1
- MRQQMVZUHXUPEV-IHRRRGAJSA-N Asp-Arg-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O MRQQMVZUHXUPEV-IHRRRGAJSA-N 0.000 description 1
- KNMRXHIAVXHCLW-ZLUOBGJFSA-N Asp-Asn-Ser Chemical compound C([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)O)N)C(=O)O KNMRXHIAVXHCLW-ZLUOBGJFSA-N 0.000 description 1
- NYQHSUGFEWDWPD-ACZMJKKPSA-N Asp-Gln-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CC(=O)O)N NYQHSUGFEWDWPD-ACZMJKKPSA-N 0.000 description 1
- KYQNAIMCTRZLNP-QSFUFRPTSA-N Asp-Ile-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(O)=O KYQNAIMCTRZLNP-QSFUFRPTSA-N 0.000 description 1
- AYFVRYXNDHBECD-YUMQZZPRSA-N Asp-Leu-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O AYFVRYXNDHBECD-YUMQZZPRSA-N 0.000 description 1
- WMLFFCRUSPNENW-ZLUOBGJFSA-N Asp-Ser-Ala Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O WMLFFCRUSPNENW-ZLUOBGJFSA-N 0.000 description 1
- MNQMTYSEKZHIDF-GCJQMDKQSA-N Asp-Thr-Ala Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O MNQMTYSEKZHIDF-GCJQMDKQSA-N 0.000 description 1
- ALMIMUZAWTUNIO-BZSNNMDCSA-N Asp-Tyr-Tyr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O ALMIMUZAWTUNIO-BZSNNMDCSA-N 0.000 description 1
- MFDPBZAFCRKYEY-LAEOZQHASA-N Asp-Val-Gln Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O MFDPBZAFCRKYEY-LAEOZQHASA-N 0.000 description 1
- GIKOVDMXBAFXDF-NHCYSSNCSA-N Asp-Val-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O GIKOVDMXBAFXDF-NHCYSSNCSA-N 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
- 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
- 108010052495 Calgranulin B Proteins 0.000 description 1
- 206010008111 Cerebral haemorrhage Diseases 0.000 description 1
- AMRLSQGGERHDHJ-FXQIFTODSA-N Cys-Ala-Arg Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O AMRLSQGGERHDHJ-FXQIFTODSA-N 0.000 description 1
- GEEXORWTBTUOHC-FXQIFTODSA-N Cys-Arg-Ser Chemical compound C(C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N)CN=C(N)N GEEXORWTBTUOHC-FXQIFTODSA-N 0.000 description 1
- NIXHTNJAGGFBAW-CIUDSAMLSA-N Cys-Lys-Ser Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N NIXHTNJAGGFBAW-CIUDSAMLSA-N 0.000 description 1
- BBQIWFFTTQTNOC-AVGNSLFASA-N Cys-Phe-Gln Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CS)N BBQIWFFTTQTNOC-AVGNSLFASA-N 0.000 description 1
- LOJYQMFIIJVETK-WDSKDSINSA-N Gln-Gln Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(O)=O LOJYQMFIIJVETK-WDSKDSINSA-N 0.000 description 1
- QKWBEMCLYTYBNI-GVXVVHGQSA-N Gln-Lys-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCC(N)=O QKWBEMCLYTYBNI-GVXVVHGQSA-N 0.000 description 1
- FQCILXROGNOZON-YUMQZZPRSA-N Gln-Pro-Gly Chemical compound NC(=O)CC[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O FQCILXROGNOZON-YUMQZZPRSA-N 0.000 description 1
- LPIKVBWNNVFHCQ-GUBZILKMSA-N Gln-Ser-Leu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O LPIKVBWNNVFHCQ-GUBZILKMSA-N 0.000 description 1
- UEILCTONAMOGBR-RWRJDSDZSA-N Gln-Thr-Ile Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O UEILCTONAMOGBR-RWRJDSDZSA-N 0.000 description 1
- LKDIBBOKUAASNP-FXQIFTODSA-N Glu-Ala-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O LKDIBBOKUAASNP-FXQIFTODSA-N 0.000 description 1
- JVSBYEDSSRZQGV-GUBZILKMSA-N Glu-Asp-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CCC(O)=O JVSBYEDSSRZQGV-GUBZILKMSA-N 0.000 description 1
- OAGVHWYIBZMWLA-YFKPBYRVSA-N Glu-Gly-Gly Chemical compound OC(=O)CC[C@H](N)C(=O)NCC(=O)NCC(O)=O OAGVHWYIBZMWLA-YFKPBYRVSA-N 0.000 description 1
- IVGJYOOGJLFKQE-AVGNSLFASA-N Glu-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N IVGJYOOGJLFKQE-AVGNSLFASA-N 0.000 description 1
- CGWHAXBNGYQBBK-JBACZVJFSA-N Glu-Trp-Tyr Chemical compound C([C@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CCC(O)=O)N)C(O)=O)C1=CC=C(O)C=C1 CGWHAXBNGYQBBK-JBACZVJFSA-N 0.000 description 1
- ZYRXTRTUCAVNBQ-GVXVVHGQSA-N Glu-Val-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N ZYRXTRTUCAVNBQ-GVXVVHGQSA-N 0.000 description 1
- UGVQELHRNUDMAA-BYPYZUCNSA-N Gly-Ala-Gly Chemical compound [NH3+]CC(=O)N[C@@H](C)C(=O)NCC([O-])=O UGVQELHRNUDMAA-BYPYZUCNSA-N 0.000 description 1
- BYYNJRSNDARRBX-YFKPBYRVSA-N Gly-Gln-Gly Chemical compound NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(O)=O BYYNJRSNDARRBX-YFKPBYRVSA-N 0.000 description 1
- SOEGEPHNZOISMT-BYPYZUCNSA-N Gly-Ser-Gly Chemical compound NCC(=O)N[C@@H](CO)C(=O)NCC(O)=O SOEGEPHNZOISMT-BYPYZUCNSA-N 0.000 description 1
- NVTPVQLIZCOJFK-FOHZUACHSA-N Gly-Thr-Asp Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(O)=O NVTPVQLIZCOJFK-FOHZUACHSA-N 0.000 description 1
- ZZWUYQXMIFTIIY-WEDXCCLWSA-N Gly-Thr-Leu Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O ZZWUYQXMIFTIIY-WEDXCCLWSA-N 0.000 description 1
- XHVONGZZVUUORG-WEDXCCLWSA-N Gly-Thr-Lys Chemical compound NCC(=O)N[C@@H]([C@H](O)C)C(=O)N[C@H](C(O)=O)CCCCN XHVONGZZVUUORG-WEDXCCLWSA-N 0.000 description 1
- UIQGJYUEQDOODF-KWQFWETISA-N Gly-Tyr-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)CN)CC1=CC=C(O)C=C1 UIQGJYUEQDOODF-KWQFWETISA-N 0.000 description 1
- GBYYQVBXFVDJPJ-WLTAIBSBSA-N Gly-Tyr-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)CN)O GBYYQVBXFVDJPJ-WLTAIBSBSA-N 0.000 description 1
- 101000685712 Homo sapiens Protein S100-A1 Proteins 0.000 description 1
- 101000693049 Homo sapiens Protein S100-A14 Proteins 0.000 description 1
- 101000685726 Homo sapiens Protein S100-A2 Proteins 0.000 description 1
- 101000685719 Homo sapiens Protein S100-A5 Proteins 0.000 description 1
- NCSIQAFSIPHVAN-IUKAMOBKSA-N Ile-Asn-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N NCSIQAFSIPHVAN-IUKAMOBKSA-N 0.000 description 1
- CIJLNXXMDUOFPH-HJWJTTGWSA-N Ile-Pro-Phe Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 CIJLNXXMDUOFPH-HJWJTTGWSA-N 0.000 description 1
- JHNJNTMTZHEDLJ-NAKRPEOUSA-N Ile-Ser-Arg Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O JHNJNTMTZHEDLJ-NAKRPEOUSA-N 0.000 description 1
- PXKACEXYLPBMAD-JBDRJPRFSA-N Ile-Ser-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)O)N PXKACEXYLPBMAD-JBDRJPRFSA-N 0.000 description 1
- QSXSHZIRKTUXNG-STECZYCISA-N Ile-Val-Tyr Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 QSXSHZIRKTUXNG-STECZYCISA-N 0.000 description 1
- 108010065920 Insulin Lispro Proteins 0.000 description 1
- LHSGPCFBGJHPCY-UHFFFAOYSA-N L-leucine-L-tyrosine Natural products CC(C)CC(N)C(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 LHSGPCFBGJHPCY-UHFFFAOYSA-N 0.000 description 1
- HXWALXSAVBLTPK-NUTKFTJISA-N Leu-Ala-Trp Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CC(C)C)N HXWALXSAVBLTPK-NUTKFTJISA-N 0.000 description 1
- OGCQGUIWMSBHRZ-CIUDSAMLSA-N Leu-Asn-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O OGCQGUIWMSBHRZ-CIUDSAMLSA-N 0.000 description 1
- ZTLGVASZOIKNIX-DCAQKATOSA-N Leu-Gln-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N ZTLGVASZOIKNIX-DCAQKATOSA-N 0.000 description 1
- LOLUPZNNADDTAA-AVGNSLFASA-N Leu-Gln-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O LOLUPZNNADDTAA-AVGNSLFASA-N 0.000 description 1
- FQZPTCNSNPWHLJ-AVGNSLFASA-N Leu-Gln-Lys Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O FQZPTCNSNPWHLJ-AVGNSLFASA-N 0.000 description 1
- HPBCTWSUJOGJSH-MNXVOIDGSA-N Leu-Glu-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O HPBCTWSUJOGJSH-MNXVOIDGSA-N 0.000 description 1
- USLNHQZCDQJBOV-ZPFDUUQYSA-N Leu-Ile-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(O)=O USLNHQZCDQJBOV-ZPFDUUQYSA-N 0.000 description 1
- NRFGTHFONZYFNY-MGHWNKPDSA-N Leu-Ile-Tyr Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 NRFGTHFONZYFNY-MGHWNKPDSA-N 0.000 description 1
- IAJFFZORSWOZPQ-SRVKXCTJSA-N Leu-Leu-Asn Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O IAJFFZORSWOZPQ-SRVKXCTJSA-N 0.000 description 1
- KIZIOFNVSOSKJI-CIUDSAMLSA-N Leu-Ser-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)O)N KIZIOFNVSOSKJI-CIUDSAMLSA-N 0.000 description 1
- LINKCQUOMUDLKN-KATARQTJSA-N Leu-Thr-Cys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CC(C)C)N)O LINKCQUOMUDLKN-KATARQTJSA-N 0.000 description 1
- AIMGJYMCTAABEN-GVXVVHGQSA-N Leu-Val-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O AIMGJYMCTAABEN-GVXVVHGQSA-N 0.000 description 1
- MSFITIBEMPWCBD-ULQDDVLXSA-N Leu-Val-Tyr Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 MSFITIBEMPWCBD-ULQDDVLXSA-N 0.000 description 1
- MPOHDJKRBLVGCT-CIUDSAMLSA-N Lys-Ala-Asn Chemical compound C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCCCN)N MPOHDJKRBLVGCT-CIUDSAMLSA-N 0.000 description 1
- KCXUCYYZNZFGLL-SRVKXCTJSA-N Lys-Ala-Leu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(O)=O KCXUCYYZNZFGLL-SRVKXCTJSA-N 0.000 description 1
- SKRGVGLIRUGANF-AVGNSLFASA-N Lys-Leu-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O SKRGVGLIRUGANF-AVGNSLFASA-N 0.000 description 1
- PDIDTSZKKFEDMB-UWVGGRQHSA-N Lys-Pro-Gly Chemical compound [H]N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O PDIDTSZKKFEDMB-UWVGGRQHSA-N 0.000 description 1
- YTJFXEDRUOQGSP-DCAQKATOSA-N Lys-Pro-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O YTJFXEDRUOQGSP-DCAQKATOSA-N 0.000 description 1
- WQDKIVRHTQYJSN-DCAQKATOSA-N Lys-Ser-Arg Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)N WQDKIVRHTQYJSN-DCAQKATOSA-N 0.000 description 1
- HDNOQCZWJGGHSS-VEVYYDQMSA-N Met-Asn-Thr Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O HDNOQCZWJGGHSS-VEVYYDQMSA-N 0.000 description 1
- BCRQJDMZQUHQSV-STQMWFEESA-N Met-Gly-Tyr Chemical compound [H]N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O BCRQJDMZQUHQSV-STQMWFEESA-N 0.000 description 1
- MNGBICITWAPGAS-BPUTZDHNSA-N Met-Ser-Trp Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O MNGBICITWAPGAS-BPUTZDHNSA-N 0.000 description 1
- GGXZOTSDJJTDGB-GUBZILKMSA-N Met-Ser-Val Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O GGXZOTSDJJTDGB-GUBZILKMSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- AUEJLPRZGVVDNU-UHFFFAOYSA-N N-L-tyrosyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CC1=CC=C(O)C=C1 AUEJLPRZGVVDNU-UHFFFAOYSA-N 0.000 description 1
- AJHCSUXXECOXOY-UHFFFAOYSA-N N-glycyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)CN)C(O)=O)=CNC2=C1 AJHCSUXXECOXOY-UHFFFAOYSA-N 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000007072 Nerve Growth Factors Human genes 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- FSPGBMWPNMRWDB-AVGNSLFASA-N Phe-Cys-Gln Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N FSPGBMWPNMRWDB-AVGNSLFASA-N 0.000 description 1
- LLGTYVHITPVGKR-RYUDHWBXSA-N Phe-Gln-Gly Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(O)=O LLGTYVHITPVGKR-RYUDHWBXSA-N 0.000 description 1
- NAXPHWZXEXNDIW-JTQLQIEISA-N Phe-Gly-Gly Chemical compound OC(=O)CNC(=O)CNC(=O)[C@@H](N)CC1=CC=CC=C1 NAXPHWZXEXNDIW-JTQLQIEISA-N 0.000 description 1
- MJQFZGOIVBDIMZ-WHOFXGATSA-N Phe-Ile-Gly Chemical compound N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)O MJQFZGOIVBDIMZ-WHOFXGATSA-N 0.000 description 1
- JXQVYPWVGUOIDV-MXAVVETBSA-N Phe-Ser-Ile Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O JXQVYPWVGUOIDV-MXAVVETBSA-N 0.000 description 1
- KLYYKKGCPOGDPE-OEAJRASXSA-N Phe-Thr-Leu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O KLYYKKGCPOGDPE-OEAJRASXSA-N 0.000 description 1
- JARJPEMLQAWNBR-GUBZILKMSA-N Pro-Asp-Arg Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O JARJPEMLQAWNBR-GUBZILKMSA-N 0.000 description 1
- UUHXBJHVTVGSKM-BQBZGAKWSA-N Pro-Gly-Asn Chemical compound [H]N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O UUHXBJHVTVGSKM-BQBZGAKWSA-N 0.000 description 1
- JMVQDLDPDBXAAX-YUMQZZPRSA-N Pro-Gly-Gln Chemical compound NC(=O)CC[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H]1CCCN1 JMVQDLDPDBXAAX-YUMQZZPRSA-N 0.000 description 1
- ULWBBFKQBDNGOY-RWMBFGLXSA-N Pro-Lys-Pro Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CCCCN)C(=O)N2CCC[C@@H]2C(=O)O ULWBBFKQBDNGOY-RWMBFGLXSA-N 0.000 description 1
- LEIKGVHQTKHOLM-IUCAKERBSA-N Pro-Pro-Gly Chemical compound OC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@H]1NCCC1 LEIKGVHQTKHOLM-IUCAKERBSA-N 0.000 description 1
- LNICFEXCAHIJOR-DCAQKATOSA-N Pro-Ser-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O LNICFEXCAHIJOR-DCAQKATOSA-N 0.000 description 1
- 102100023097 Protein S100-A1 Human genes 0.000 description 1
- 102100029796 Protein S100-A10 Human genes 0.000 description 1
- 102100029812 Protein S100-A12 Human genes 0.000 description 1
- 102100026298 Protein S100-A14 Human genes 0.000 description 1
- 102100023089 Protein S100-A2 Human genes 0.000 description 1
- 102100023087 Protein S100-A4 Human genes 0.000 description 1
- 102100023088 Protein S100-A5 Human genes 0.000 description 1
- 102100032446 Protein S100-A7 Human genes 0.000 description 1
- 102100032420 Protein S100-A9 Human genes 0.000 description 1
- 108010005256 S100 Calcium Binding Protein A7 Proteins 0.000 description 1
- 108010085149 S100 Calcium-Binding Protein A4 Proteins 0.000 description 1
- 108010015695 S100 calcium binding protein A10 Proteins 0.000 description 1
- 108700016890 S100A12 Proteins 0.000 description 1
- 101150097337 S100A12 gene Proteins 0.000 description 1
- 101150026440 S100b gene Proteins 0.000 description 1
- YQHZVYJAGWMHES-ZLUOBGJFSA-N Ser-Ala-Ser Chemical compound OC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O YQHZVYJAGWMHES-ZLUOBGJFSA-N 0.000 description 1
- HBOABDXGTMMDSE-GUBZILKMSA-N Ser-Arg-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(O)=O HBOABDXGTMMDSE-GUBZILKMSA-N 0.000 description 1
- HEQPKICPPDOSIN-SRVKXCTJSA-N Ser-Asp-Tyr Chemical compound OC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 HEQPKICPPDOSIN-SRVKXCTJSA-N 0.000 description 1
- FMDHKPRACUXATF-ACZMJKKPSA-N Ser-Gln-Ser Chemical compound OC[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(O)=O FMDHKPRACUXATF-ACZMJKKPSA-N 0.000 description 1
- KJMOINFQVCCSDX-XKBZYTNZSA-N Ser-Gln-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KJMOINFQVCCSDX-XKBZYTNZSA-N 0.000 description 1
- YMTLKLXDFCSCNX-BYPYZUCNSA-N Ser-Gly-Gly Chemical compound OC[C@H](N)C(=O)NCC(=O)NCC(O)=O YMTLKLXDFCSCNX-BYPYZUCNSA-N 0.000 description 1
- KDGARKCAKHBEDB-NKWVEPMBSA-N Ser-Gly-Pro Chemical compound C1C[C@@H](N(C1)C(=O)CNC(=O)[C@H](CO)N)C(=O)O KDGARKCAKHBEDB-NKWVEPMBSA-N 0.000 description 1
- RIAKPZVSNBBNRE-BJDJZHNGSA-N Ser-Ile-Leu Chemical compound OC[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O RIAKPZVSNBBNRE-BJDJZHNGSA-N 0.000 description 1
- UIPXCLNLUUAMJU-JBDRJPRFSA-N Ser-Ile-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(O)=O UIPXCLNLUUAMJU-JBDRJPRFSA-N 0.000 description 1
- FUMGHWDRRFCKEP-CIUDSAMLSA-N Ser-Leu-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O FUMGHWDRRFCKEP-CIUDSAMLSA-N 0.000 description 1
- QYSFWUIXDFJUDW-DCAQKATOSA-N Ser-Leu-Arg Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O QYSFWUIXDFJUDW-DCAQKATOSA-N 0.000 description 1
- KCGIREHVWRXNDH-GARJFASQSA-N Ser-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CO)N KCGIREHVWRXNDH-GARJFASQSA-N 0.000 description 1
- YUJLIIRMIAGMCQ-CIUDSAMLSA-N Ser-Leu-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O YUJLIIRMIAGMCQ-CIUDSAMLSA-N 0.000 description 1
- NMZXJDSKEGFDLJ-DCAQKATOSA-N Ser-Pro-Lys Chemical compound C1C[C@H](N(C1)C(=O)[C@H](CO)N)C(=O)N[C@@H](CCCCN)C(=O)O NMZXJDSKEGFDLJ-DCAQKATOSA-N 0.000 description 1
- AZWNCEBQZXELEZ-FXQIFTODSA-N Ser-Pro-Ser Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O AZWNCEBQZXELEZ-FXQIFTODSA-N 0.000 description 1
- 241000519995 Stachys sylvatica Species 0.000 description 1
- GLQFKOVWXPPFTP-VEVYYDQMSA-N Thr-Arg-Asp Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O GLQFKOVWXPPFTP-VEVYYDQMSA-N 0.000 description 1
- UKBSDLHIKIXJKH-HJGDQZAQSA-N Thr-Arg-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O UKBSDLHIKIXJKH-HJGDQZAQSA-N 0.000 description 1
- KRPKYGOFYUNIGM-XVSYOHENSA-N Thr-Asp-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N)O KRPKYGOFYUNIGM-XVSYOHENSA-N 0.000 description 1
- MECLEFZMPPOEAC-VOAKCMCISA-N Thr-Leu-Lys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)O)N)O MECLEFZMPPOEAC-VOAKCMCISA-N 0.000 description 1
- KRDSCBLRHORMRK-JXUBOQSCSA-N Thr-Lys-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O KRDSCBLRHORMRK-JXUBOQSCSA-N 0.000 description 1
- SPVHQURZJCUDQC-VOAKCMCISA-N Thr-Lys-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O SPVHQURZJCUDQC-VOAKCMCISA-N 0.000 description 1
- WRUWXBBEFUTJOU-XGEHTFHBSA-N Thr-Met-Ser Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CO)C(=O)O)N)O WRUWXBBEFUTJOU-XGEHTFHBSA-N 0.000 description 1
- DEGCBBCMYWNJNA-RHYQMDGZSA-N Thr-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)[C@@H](C)O DEGCBBCMYWNJNA-RHYQMDGZSA-N 0.000 description 1
- PRTHQBSMXILLPC-XGEHTFHBSA-N Thr-Ser-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O PRTHQBSMXILLPC-XGEHTFHBSA-N 0.000 description 1
- IEZVHOULSUULHD-XGEHTFHBSA-N Thr-Ser-Val Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O IEZVHOULSUULHD-XGEHTFHBSA-N 0.000 description 1
- NJGMALCNYAMYCB-JRQIVUDYSA-N Thr-Tyr-Asn Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(N)=O)C(O)=O NJGMALCNYAMYCB-JRQIVUDYSA-N 0.000 description 1
- JAWUQFCGNVEDRN-MEYUZBJRSA-N Thr-Tyr-Leu Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC(C)C)C(=O)O)N)O JAWUQFCGNVEDRN-MEYUZBJRSA-N 0.000 description 1
- KZTLZZQTJMCGIP-ZJDVBMNYSA-N Thr-Val-Thr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KZTLZZQTJMCGIP-ZJDVBMNYSA-N 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 208000030886 Traumatic Brain injury Diseases 0.000 description 1
- SVGAWGVHFIYAEE-JSGCOSHPSA-N Trp-Gly-Gln Chemical compound C1=CC=C2C(C[C@H](N)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(O)=O)=CNC2=C1 SVGAWGVHFIYAEE-JSGCOSHPSA-N 0.000 description 1
- UJRIVCPPPMYCNA-HOCLYGCPSA-N Trp-Leu-Gly Chemical compound CC(C)C[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)N UJRIVCPPPMYCNA-HOCLYGCPSA-N 0.000 description 1
- FBVGQXJIXFZKSQ-GMVOTWDCSA-N Tyr-Ala-Trp Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CC3=CC=C(C=C3)O)N FBVGQXJIXFZKSQ-GMVOTWDCSA-N 0.000 description 1
- QUILOGWWLXMSAT-IHRRRGAJSA-N Tyr-Gln-Gln Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O QUILOGWWLXMSAT-IHRRRGAJSA-N 0.000 description 1
- NKUGCYDFQKFVOJ-JYJNAYRXSA-N Tyr-Leu-Gln Chemical compound NC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 NKUGCYDFQKFVOJ-JYJNAYRXSA-N 0.000 description 1
- JIODCDXKCJRMEH-NHCYSSNCSA-N Val-Arg-Gln Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N JIODCDXKCJRMEH-NHCYSSNCSA-N 0.000 description 1
- CFSSLXZJEMERJY-NRPADANISA-N Val-Gln-Ala Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(O)=O CFSSLXZJEMERJY-NRPADANISA-N 0.000 description 1
- FTKXYXACXYOHND-XUXIUFHCSA-N Val-Ile-Leu Chemical compound CC(C)[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O FTKXYXACXYOHND-XUXIUFHCSA-N 0.000 description 1
- DIOSYUIWOQCXNR-ONGXEEELSA-N Val-Lys-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)NCC(O)=O DIOSYUIWOQCXNR-ONGXEEELSA-N 0.000 description 1
- IECQJCJNPJVUSB-IHRRRGAJSA-N Val-Tyr-Ser Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CO)C(O)=O IECQJCJNPJVUSB-IHRRRGAJSA-N 0.000 description 1
- OWFGFHQMSBTKLX-UFYCRDLUSA-N Val-Tyr-Tyr Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)O)N OWFGFHQMSBTKLX-UFYCRDLUSA-N 0.000 description 1
- 108010081404 acein-2 Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 108010050025 alpha-glutamyltryptophan Proteins 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 108010013835 arginine glutamate Proteins 0.000 description 1
- 108010077245 asparaginyl-proline Proteins 0.000 description 1
- 108010069205 aspartyl-phenylalanine Proteins 0.000 description 1
- 210000001130 astrocyte Anatomy 0.000 description 1
- 239000003150 biochemical marker Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000004094 calcium homeostasis Effects 0.000 description 1
- 238000007675 cardiac surgery Methods 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 206010008118 cerebral infarction Diseases 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000013373 clone screening Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 208000035250 cutaneous malignant susceptibility to 1 melanoma Diseases 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000005002 finish coating Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 108010063718 gamma-glutamylaspartic acid Proteins 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 108010078144 glutaminyl-glycine Proteins 0.000 description 1
- 108010057083 glutamyl-aspartyl-leucine Proteins 0.000 description 1
- 108010067216 glycyl-glycyl-glycine Proteins 0.000 description 1
- 108010048994 glycyl-tyrosyl-alanine Proteins 0.000 description 1
- 108010010147 glycylglutamine Proteins 0.000 description 1
- 108010084389 glycyltryptophan Proteins 0.000 description 1
- 108010037850 glycylvaline Proteins 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 230000000302 ischemic effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 108010012058 leucyltyrosine Proteins 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 239000003900 neurotrophic factor Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 108010070409 phenylalanyl-glycyl-glycine Proteins 0.000 description 1
- 108010073025 phenylalanylphenylalanine Proteins 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108010020755 prolyl-glycyl-glycine Proteins 0.000 description 1
- 108010087846 prolyl-prolyl-glycine Proteins 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 108010061238 threonyl-glycine Proteins 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 108010038745 tryptophylglycine Proteins 0.000 description 1
- 108010044292 tryptophyltyrosine Proteins 0.000 description 1
- 108010035534 tyrosyl-leucyl-alanine Proteins 0.000 description 1
- 108010073969 valyllysine Proteins 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
本发明涉及生物技术领域,公开了鼠抗人S100B蛋白的单克隆抗体OTI6A6和OTI3G6,所述的抗体是由保藏号分别为:CGMCCNo.22344和CGMCCNo.22345的杂交瘤细胞株OTI6A6和OTI3G6分泌产生。所述鼠单克隆抗体OTI6A6和OTI3G6的免疫原为真核细胞293T表达的S100B全长蛋白,这2个鼠单克隆抗体可以识别人S100B蛋白表面的不同抗原决定簇,其OTI6A6抗体轻链(VL)的氨基酸序列如SEQIDNO.1所示;OTI6A6抗体的重链(VH)的氨基酸序列如SEQIDNo.2所示。其OTI3G6轻链(VL)的氨基酸序列如SEQIDNO.11所示;OTI3G6的重链(VH)的氨基酸序列如SEQIDNo.12所示。本发明的抗人S100B蛋白鼠单克隆抗体可应用于检测S100B蛋白的各种免疫检测试剂盒的制备,包括但不限于双抗体夹心ELISA或化学发光法试剂盒的制备,为临床上评估脑损伤的程度和预后提供辅助诊断。
Description
技术领域
本发明属于生物技术领域,尤其涉及抗人S100B蛋白鼠单克隆抗体及其在免疫检测方面应用。
背景技术
S100B蛋白又叫中枢神经特异蛋白,由2个β亚基通过半胱氨酸残基形成二硫键,以二聚体活性形式存在于神经系统中,是分子量为21KD的酸性钙结合蛋白,主要由星形胶质细胞产生。
S100B蛋白具有广泛的生物学活性,在细胞增生、分化、基因表达、细胞凋亡中具有重要作用。生理状态下脑中S100B蛋白在胚胎期第14d就有微弱表达,随后与神经系统生长发育呈平行增加的关系,成年后相对稳定。正常成人血清中含量小于0.2ng/mL,生理状态下是一种神经营养因子,影响神经胶质细胞的生长、增殖、分化,维持钙稳态,并对学习记忆等发挥一定作用,促进脑的发育;当人在脑梗塞、脑外伤或心脏外科手术时,S100B蛋白从胞液中渗出进入脑脊液,再经受损的血脑屏障进入血液,从而导致血液中S100B蛋白的浓度升高。
近年来,S100B被广泛应用于评价脑外伤、脑出血、缺血性脑血管病、麻醉、休克等引起的脑损伤,也是恶性黑色素瘤标志物。S100B蛋白作为脑损伤的生化标志物在脑损伤后有一定的时间变化规律,而且与脑损伤程度及预后又紧密相关,稳定性较好,其浓度值的检测有助于临床上判断神经组织的病灶大小、治疗效果和判断人的预后等。
中国专利关于S100B蛋白单克隆抗体制备的条目基本没有,申请公布号CN111487409 A涉及S100B蛋白化学发光检测试剂盒及其使应方法,未提到抗体的制备方法也没有公开抗体CDR序列。申请公布号CN 111487407 A也涉及一种S100B蛋白的检测试剂盒及其使用方法,未提到抗体的制备方法也没有公开抗体CDR序列。国际专利申请利用S100B或S100B+monoclonal antibody作为关键词,有关与S100B具有高亲和力和特异性结合的单克隆抗体的专利没有查到,有关检测利用抗S100B鼠单克隆抗体OTI6A6和OTI3G6制备免疫检测试剂盒也未查到。
发明内容
本发明的目的是提供特异性好、亲和力高的抗人S100B蛋白鼠单克隆抗体OTI6A6和OTI3G6及其在双抗体夹心ELISA或化学发光法制备的免疫检测试剂盒中的应用,为临床上评估脑损伤的程度和预后提供辅助诊断。
所述鼠单克隆抗体为鼠单克隆抗体OTI6A6或鼠单克隆抗体OTI3G6。
所述鼠单克隆抗体OTI6A6和鼠单克隆抗体OTI3G6以真核细胞293T表达的S100B全长蛋白为免疫原,通过免疫注射小鼠,取免疫小鼠脾脏制备悬液,并使之与骨髓瘤细胞进行细胞融合获得。
所述鼠单克隆抗体OTI6A6,其轻链可变区(VL)含112aa,其氨基酸序列如SEQ IDNO.1所示;所述抗体的重链(VH)含116aa,其氨基酸序列如SEQ IDNo.2所示。
所述鼠单克隆抗体OTI6A6,其中VL区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为27aa-38aa,56aa-58aa和95aa-101aa。其氨基酸序列分别如SEQ ID No.3-5所示。
所述鼠单克隆抗体OTI6A6,其中VH区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为26aa-35aa,53aa-62aa和101aa-105aa。其氨基酸残基序列分别如SEQ ID No.6-8所示。
所述鼠单克隆抗体OTI3G6,其轻链可变区(VL)含110aa,其氨基酸序列如SEQ IDNO.11所示;所述抗体的重链(VH)含113aa,其氨基酸序列如SEQ IDNo.12所示。
所述鼠单克隆抗体OTI3G6,其中VL区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为27aa-37aa,55aa-57aa和94aa-99aa。其氨基酸序列分别如SEQ ID No.13-15所示。
所述鼠单克隆抗体OTI3G6,其中VH区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为26aa-34aa,52aa-58aa和97aa-102aa。其氨基酸残基序列分别如SEQ ID No.16-18所示。
所述的鼠单克隆抗体包括鼠单克隆抗体OTI6A6或鼠单克隆抗体OTI3G6可以与S100B蛋白高特异性结合,可通过本领域技术人员所公知的的方法,制备成检测S100B蛋白的各种免疫检测试剂盒。尤其是,应用于双抗体夹心ELISA或化学发光法制备的免疫检测试剂盒中。双抗体夹心ELISA检测S100B蛋白标准品,检测灵敏度能达到62.5pg/ml,标准曲线的R2=0.9934,推出样品浓度计算公式:y=0.0045x+0.1622。板式化学发光法检测S100B蛋白标准品,检测灵敏度能达到30pg/ml。
保藏信息
用于保藏的杂交瘤细胞株OTI6A6和OTI3G6的分类命名为:抗S100B鼠单克隆抗体的杂交瘤细胞株;
保藏单位全称:中国微生物菌种保藏管理委员会普通微生物中心;
保藏单位简称:CGMCC;
保藏单位地址:北京市朝阳区北辰西路1号院3号中国科学院微生物研究所;
保藏日期:2021年5月21日;
保藏编号:CGMCC No.22344和CGMCC No.22345
附图说明
图1为鼠单克隆抗体OTI6A6和OTI3G6重链和轻链可变区扩增产物的电泳图,M为DNA分子量Marker。
图2为单克隆抗体OTI6A6特异识别完整的S100B(Full length S100B,S100B-FL)蛋白的Western blot检测结果图。泳道1到泳道13分别为S100B、S100A1、S100A2、S100A3、S100A4、S100A5、S100A6、S100A7、S100A9、S100A10、S100A12、S100A14、S100P蛋白;
图3为单克隆抗体OTI3G6特异识别完整的S100B(Full length S100B,S100B-FL)蛋白的Western blot检测结果图。泳道1到泳道13分别为S100B、S100A1、S100A2、S100A3、S100A4、S100A5、S100A6、S100A7、S100A9、S100A10、S100A12、S100A14、S100P蛋白;
图4为双抗体夹心ELISA法检测S100B标准曲线,横坐标为S100B蛋白浓度,纵坐标为检测的OD值。标准曲线的R2=0.9934,推出样品浓度计算公式:y=0.0045x+0.1622。
图5为板式化学发光检测S100B蛋白,横坐标为S100B蛋白浓度,纵坐标为检测的化学发光读数。
具体实施方式
下面结合具体实施例,进一步阐述本发明。应理解,这些实施例仅用于说明本发明而不用于限制本发明的范围。下列实施例中未注明具体条件的实验方法,通常按照常规条件、实验室手册中所述的条件或按照制造厂商所建议的条件。
实施例1:抗S100B蛋白鼠单克隆抗体的制备
一、S100B重组蛋白的生产
从Genebank中选调S100B基因NM_006272(NP_006263),该基因编码S100B全长92个氨基酸(aa)。以从美国傲锐东源生物科技有限公司获得的质粒RC200277(含S100B ORF276bp)为模板,设计两条引物并分别引入酶切位点SgfI和MluI,克隆入表达载体pCMV6-Entry,建立S100B重组表达质粒。采用本领域技术人员所知的技术方法将该基因转染HEK293T细胞,转染48小时后,进行细胞裂解,收集所有培养皿中的裂解液,4℃离心,DDK亲和层析柱纯化,SDS-PAGE电泳鉴定纯度。电泳后从凝胶上观察到分子量为10.5kDa左右的目的蛋白条带,纯度在85%以上,达到了制备单抗的纯度要求。
二、动物免疫
上述纯化的S100B重组蛋白以完全弗氏佐剂乳化,采用皮下或腹腔注射方法免疫6-8周龄BALB/c小鼠,免疫剂量为60μg/只,间隔两周后进行第二次免疫,以不完全弗氏佐剂乳化,免疫剂量为30μg/只。免疫两次后取尾血以ELISA法梯度稀释测定血清效价;依据ELISA效价12800时的OD450大于2.0为标准,根据结果判定是否加强免疫或是继续免疫,选取抗体效价最高的小鼠进行细胞融合。
三、细胞融合、克隆筛选取免疫小鼠脾细胞与小鼠骨髓瘤细胞SP2/0融合,脾细胞与SP2/0细胞以10:1混合,以50%PEG4,000介导融合,离心后重悬于HAT选择性培养基中,接种于含有饲养细胞的96孔微孔板中,置37℃、5%CO2培养箱培养,融合后第4d、第7d用HAT培养液半量换液,10d后换用HT培养液。观察杂交瘤细胞的生长情况,等克隆长至孔底面积的1/4~1/3,取培养上清液,用ELISA法进行抗体检测,筛选阳性克隆。以有限稀释法对阳性孔的杂交瘤细胞进行克隆化培养,直到克隆化细胞抗体阳性率为100%,选岀高分泌特异性细胞株OTI6A6和OTI3G6(即ELISA效价在1:104以上的阳性克隆),此时可将阳性克隆细胞进一步扩大培养,直至细胞系能稳定分泌单克隆抗体。
四、单克隆抗体的制备和纯化以无血清培养基悬浮培养杂交瘤细胞,1周左右当细胞上清体积扩培到任务量及细胞死亡率达到60%-70%时,收取细胞悬液,离心取上清,亲和层析法进行抗体纯化,根据抗体亚型选择相应柱料进行纯化。以BCA法测定纯化后的单抗浓度,再分装、冻存。
实施例2:抗S100B蛋白鼠单克隆抗体OTI6A6或OTI3G6的鉴定
一、鼠单克隆抗体的特异性鉴定
采用Western blot(WB)检测。S100B、S100A1、S100A2、S100A3、S100A4、S100A5、S100A6、S100A7、S100A9、S100A10、S100A12、S100A14、S100P等蛋白上样进行SDS-PAGE,蛋白上样量为20ng,转膜后进行WB检测。
结果显示,鼠单克隆抗体OTI6A6或OTI3G6仅特异识别S100B蛋白,而不识别其它12种S100家族蛋白,说明本发明的抗S100B蛋白鼠单克隆抗体OTI6A6或OTI3G6对S100B蛋白具有高度特异性。结果见图2和图3。
二、鼠单克隆抗体的效价
采用倍比稀释法稀释鼠单克隆抗体OTI6A6或OTI3G6,用间接ELISA测抗体效价,结果显示本发明涉及的鼠单克隆抗体OTI6A6或OTI3G6效价均为2×106。
三、抗体配对
为了挑选包被抗体和检测抗体的最佳组合,采用棋盘组合,将获得的多株S100B鼠单克隆抗体相互配对。基本步骤:多株单克隆抗体分别包被酶标板,4℃过夜。第二天取出酶标板,PBST洗涤一次,1%BSA溶液37℃封闭2小时,PBST洗涤3次;每孔加入S100B全长蛋白100μl,浓度分别为20、5、1和0ng/ml,37℃孵育1小时;孵育完成后取出酶标板,PBST洗涤3次,分别加入HRP标记的上述多株抗体作为检测抗体,37℃孵育1小时。PBST洗涤5次,加入TMB底物,37℃显色10min。取出后加终止液,在酶标仪上测定OD450读数。根据样品的OD值与阴性对照的背景值,选出最为理想的鼠单克隆抗体对,配对筛选结果见表1。本发明涉及的抗体OTI6A6作为包被抗体,抗体OTI3G6作为检测抗体最佳。抗人S100B蛋白鼠单克隆抗体OTI6A6和鼠单克隆抗体OTI3G6,可以识别人S100B蛋白表面的不同抗原决定簇。
表1抗体配对实验筛选结果
实施例3:鼠单克隆抗体OTI6A6或OTI3G6可变区基因与氨基酸序列分析
从Takara Bio USA公司采购RACE 5’/3’试剂盒,采用5’RACE(RapidAmplification of cDNA Ends,快速扩增cDNA末端)技术扩增杂交瘤细胞功能性抗体的可变区轻链与重链基因序列。具体实验过程参见Takara Bio USA公司RACE 5’/3’Kit使用者手册。
根据所述的抗体OTI6A6或OTI3G6为IgG1亚型,设计针对其Ig和Kapa恒定区3’端的特异性基因引物pRace-H-GSP和pRace-K-GSP,引物序列如下:
pRace-H-GSP:CATCDGTCTATCCACTGGCCCCTG
pRace-K-GSP:CTTCCCACCATCCAGTGAGCAGTT
从杂交瘤细胞OTI6A6或OTI3G6中提取mRNA,逆转录为cDNA,RACE扩增抗体重链和轻链的DNA片段,扩增产物如图1所示,基因大小符合鼠抗体特征。分别将扩增的轻链与重链通过酶切连于克隆载体PUC119,通过蓝白斑挑取阳性克隆,纯化阳性质粒进行测序,采用测序仪ABI 3730,测序引物为通用引物M13f和M13r。
利用互联网,在http://www.imgt.org上使用IMGT/V-QUEST分析软件,分别将轻链与重链的核苷酸序列进行测序结果数据分析,得到鼠单克隆抗体OTI6A6的轻链氨基酸序列如SEQ ID NO.1所示,重链氨基酸序列如SEQ ID NO.2所示。VL全长为112个氨基酸,其FR的4个结构域氨基酸数分别为26、17、36和11,CDR的3个结构域氨基酸数分别为12、3和7,CDR1、CDR2和CDR3的区域相应地分别为27aa-38aa,56aa-58aa和95aa-101aa,其氨基酸序列分别:QSLLNSNNQKNY、LAS、QQYYSIP。
通过分析,所述鼠单克隆抗体OTI6A6VH全长为116个氨基酸,其FR的4个结构域氨基酸数分别为25、17、38和11,CDR的3个结构域氨基酸数分别为10、10和5,CDR1、CDR2和CDR3分别为26aa-35aa,53aa-62aa和101aa-105aa,其氨基酸序列分别为GFTFTFSDYY、INTKANGYTT、AREGG。
通过分析,所述鼠单克隆抗体OTI3G6的轻链氨基酸序列如SEQ ID NO.11所示,重链氨基酸序列如SEQ ID NO.12所示。VL全长为110个氨基酸,其FR的4个结构域氨基酸数分别为26、17、36和11,CDR的3个结构域氨基酸数分别为11、3和6,CDR1、CDR2和CDR3的区域相应地分别为27aa-37aa,55aa-57aa和94aa-99aa,其氨基酸序列分别:QTIVYSNGNTY、WAS、FQGSHV。
通过分析,所述鼠单克隆抗体OTI3G6 VH全长为113个氨基酸,其FR的4个结构域氨基酸数分别为25、17、38和11,CDR的3个结构域氨基酸数分别为9、7和6,CDR1、CDR2和CDR3分别为25aa-34aa,52aa-58aa和97aa-102aa,其氨基酸序列分别为GYSITSGYA、INYSGET、ARANDA。
实施例4:抗S100B蛋白鼠单克隆抗体OTI6A6或OTI3G6用于制备双抗体夹心ELISA检测试剂盒
采用本领域技术人员所公知的基于ELISA双抗体夹心法原理的检测技术,制作S100B蛋白检测试剂盒,用于临床上与S100B蛋白相关疾病辅助诊断。
一、试剂盒组成
1.包被OTI6A6抗体的板条:用PBS缓冲液稀释抗体至5μg/ml,包被到微孔板上,每孔100μl,4℃孵育过夜,以PBST洗涤3次,甩干;加含有1%BSA、5%蔗糖、0.05%Proclin300的PBS进行封闭,37℃反应2h,弃去孔内封闭液,甩干;将包被板置于37℃烘箱2h,即完成包被,以铝箔袋密封,存放于4℃保存备用。
2.试剂配制
1.酶结合物配制采用简易过碘酸钠法标记抗S100B蛋白鼠单克隆抗体OTI3G6,滴定工作浓度,配制成1:20的浓缩液,稀释液为含1%BSA、5%甘油、0.05%Proclin 300的PBS,过滤除菌。
2.洗涤缓冲液为常规的pH7.4的PBST,含0.05%Proclin300,配制成20倍浓缩液。
3.酶的底物液(显色液)单组分TMB(从市场采购)。
4.样本稀释液含1%BSA、0.05%Proclin 300的PBS,过滤除菌。
5.终止液用10ml HCl(36-38%)加入110ml蒸馏水中,混合过程需缓慢进行,配置成1N的HCl。
6.标准品全长S100B蛋白,以含1%BSA、5%蔗糖、10%甘油、0.05%Proclin300的PBS为稀释液,将样品稀释为20μg/ml,过滤除菌,无菌分装。
二、检测操作要点
1、为保证检测结果的准确性,建议标准品及样本均设双孔测定。每次检测均需做标准曲线。
2、如标本中待测物质含量过高,请先用样本稀释液进行稀释,以使样本符合试剂盒的检测范围,最后计算时再乘以相应的稀释倍数。
3、加样:加样时,请使用一次性的洁净吸头,避免交叉污染。加样时应尽量轻缓,避免起泡,将样本加于酶标板孔底部,切勿沿孔壁加样。
4、温育:为防止样本蒸发或污染,温育过程中酶标板必须覆上板贴,实验过程中酶标板应避免处于干燥的状态。温育过程中应随时观察温箱温度是否恒定于37℃,及时调整。温育过程中,温箱不易开启太多次,以免影响温度平衡。
5、洗涤:洗涤过程非常重要,不充分的洗涤易造成假阳性。
6、显色:为保证实验结果的准确性,底物反应时间到后应尽快加入终止液。可在加入底物溶液后每隔一段时间观察一下显色情况以控制反应时间(比如每隔10分钟)。当肉眼可见标准品前3-4孔有明显梯度蓝色,后3-4孔显色不明显时,即可加入终止液终止反应,此时蓝色立刻变为黄色。终止液的加入顺序应尽量与底物溶液的加入顺序相同。
7、底物溶液应为浅蓝色或无色,如果颜色严重变深则必须弃用。底物溶液易受污染,请避光妥善保存。
三、标准S100B蛋白不同稀释度标准曲线的确定
上述制备的检测S100B蛋白双抗体夹心ELISA检测试剂盒从4度冰箱取出,平衡到室温。将标准品用PBS从20μg/ml稀释到500pg/ml,制备成0、31.25、62.5、125、250、500pg/ml共6个系列浓度标准品,按照上述的检测方法在每孔中加入标准品100μl,进行温育,然后弃去液体,加入HRP标记的检测抗体工作液进行温育,弃去孔内液体,甩干后每孔加入底物溶液,避光显色后每孔加入终止溶液,在反应终止后5分钟内用酶标仪在450nm波长依序测量各孔的光密度OD值做标准曲线,结果见表2。依据表2制作S100B检测标准曲线,见附图4。标准曲线的R2=0.9934,推出样品浓度计算公式:y=0.0045x+0.1622。
表2.双抗体夹心ELISA法检测S100B标准蛋白
实施例5:抗S100B蛋白鼠单克隆抗体OTI6A6或OTI3G6用于板式化学发光免疫检测
采用本领域技术人员所公知的基于双抗体夹心法原理的板式化学发光免疫检测技术,制作S100B蛋白检测试剂盒,用于临床上与S100B蛋白相关疾病辅助诊断。
一、检测原理与方法
采用基于双抗体夹心法原理的板式化学发光免疫检测技术。在不透光的白色微孔板内包被抗S100B蛋白的鼠单克隆抗体OTI6A6,将封闭好的包被板放置到科斯迈全自动发化学发光仪上,设置仪器程序,将标准品或待测标本与辣根过氧化物酶(HRP)标记的鼠单克隆抗体OTI3G6同时进行温育,则三者形成抗体-抗原-抗体复合物。洗涤除去未结合的物质,加入北京科跃中楷化学发光底物,反应10min后科斯迈全自动发化学发光仪显示化学发光值。依据信噪比大于2.0判读结果为阳性。发光值的大小反映了结合的酶标抗体的量,它与标本中的S100B蛋白的浓度成正比。根据所测定的标准品的发光值绘制标准曲线,待测标本中的S100B蛋白浓度值即可从标准曲线上获得。
二、检测S100B蛋白的板式化学发光检测试剂盒组成
1.包被OTI6A6抗体的板条:同实施例4。
2.试剂配制
1.酶结合物配制同实施例4。
2.洗涤缓冲液同实施例4。
3.化学发光显色液A液和B液,购于北京科跃中楷生物技术有限公司。
4.样本稀释液含1%BSA、0.05%Proclin 300的PBST,过滤除菌。
5.标准品S100B全长蛋白,以含1%BSA、5%蔗糖、10%甘油、0.05%Proclin300的PBS为稀释液,将样品稀释为5μg/ml,过滤除菌,无菌分装。
三、对S100B全长蛋白的检测
把S100B全长蛋白进行梯度稀释,分别为0pg/ml、10pg/ml、30pg/ml、100pg/ml、300pg/ml、1000pg/ml,以OTI6A6抗体为包被抗体、OTI3G6抗体为检测抗体,用上述检测方法检测6个梯度稀释的抗原,检测结果见表3。根据信噪比大于2.0判定,本发明所述的鼠单克隆抗体用于板式化学发光免疫检测试剂,最低灵敏度小于30pg/ml,线性范围为10-1000pg/ml。依据表3制作S100B蛋白检测标准曲线,见图5
表3.板式化学发光法检测S100B全长蛋白
0序列表
<110> 无锡傲锐东源生物科技有限公司
<120> 抗人S100B蛋白鼠单克隆抗体及其应用
<140> 2021108282843
<160> 8
<170> SIPOSequenceListing 1.0
<210> 1
<211> 112
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 1
Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ala Met Ser Val Gly
1 5 10 15
Gln Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser
20 25 30
Asn Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
35 40 45
Ser Pro Lys Leu Leu Val Tyr Leu Ala Ser Thr Arg Glu Ser Gly Val
50 55 60
Pro Asp Arg Phe Ile Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
65 70 75 80
Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln
85 90 95
Tyr Tyr Ser Ile Pro Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg
100 105 110
<210> 2
<211> 116
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 2
Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
1 5 10 15
Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Phe Thr Phe Thr Phe Ser
20 25 30
Asp Tyr Tyr Met Ser Trp Val Arg Gln Pro Pro Gly Lys Ala Leu Glu
35 40 45
Trp Leu Gly Leu Ile Asn Thr Lys Ala Asn Gly Tyr Thr Thr Glu Tyr
50 55 60
Ser Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Gln
65 70 75 80
Ser Ile Leu Tyr Leu Gln Met Asn Thr Leu Arg Ala Glu Asp Ser Ala
85 90 95
Thr Tyr Tyr Cys Ala Arg Glu Gly Gly Trp Gly Gln Gly Thr Leu Val
100 105 110
Thr Val Ser Val
115
<210> 3
<211> 12
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 3
Gln Ser Leu Leu Asn Ser Asn Asn Gln Lys Asn Tyr
1 5 10
<210> 4
<211> 3
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 4
Leu Ala Ser
1
<210> 5
<211> 7
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 5
Gln Gln Tyr Tyr Ser Ile Pro
1 5
<210> 6
<211> 10
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 6
Gly Phe Thr Phe Thr Phe Ser Asp Tyr Tyr
1 5 10
<210> 7
<211> 10
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 7
Ile Asn Thr Lys Ala Asn Gly Tyr Thr Thr
1 5 10
<210> 8
<211> 5
<212> PRT
<213> Mus musculus x Rattus norvegicus
<400> 8
Ala Arg Glu Gly Gly
1 5
<120> 抗人S100B蛋白鼠单克隆抗体及其应用
<140> 2021108282843
<141> 2021--0-7-
<160> 8
<170> SIPOSequenceListing 1.0
<210> 1
<211> 110
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 1
Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ile Leu Gly
1 5 10 15
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Thr Ile Val Tyr Ser
20 25 30
Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser
35 40 45
Pro Lys Pro Leu Ile Tyr Trp Ala Ser Asn Arg Phe Ser Gly Val Pro
50 55 60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65 70 75 80
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly
85 90 95
Ser His Val Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg
100 105 110
<210> 2
<211> 113
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 2
Asp Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln
1 5 10 15
Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly
20 25 30
Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp
35 40 45
Met Gly Tyr Ile Asn Tyr Ser Gly Glu Thr Thr Tyr Asn Pro Ser Leu
50 55 60
Lys Ser Arg Phe Ser Ile Thr Arg Asp Thr Ser Arg Asn Gln Phe Phe
65 70 75 80
Leu Gln Leu Asn Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys
85 90 95
Ala Arg Ala Asn Asp Ala Trp Gly Gln Gly Thr Ser Val Thr Val Ser
100 105 110
Ser
<210> 3
<211> 11
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 3
Gln Thr Ile Val Tyr Ser Asn Gly Asn Thr Tyr
1 5 10
<210> 4
<211> 3
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 4
Trp Ala Ser
1
<210> 5
<211> 6
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 5
Phe Gln Gly Ser His Val
1 5
<210> 6
<211> 9
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 6
Gly Tyr Ser Ile Thr Ser Gly Tyr Ala
1 5
<210> 7
<211> 7
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 7
Ile Asn Tyr Ser Gly Glu Thr
1 5
<210> 8
<211> 6
<212> PRT
<213> Musmusculus x Rattusnorvegicus
<400> 8
Ala Arg Ala Asn Asp Ala
1 5
Claims (11)
1.两株分泌抗人S100B的单克隆抗体的杂交瘤细胞株OTI6A6和OTI3G6,于2021年5月21日保藏于中国微生物菌种保藏管理委员会普通微生物中心,保藏编号分别为CGMCCNo.22344和CGMCC No.22345。
2.由权利要求1所述的保藏编号为CGMCC No.22344和CGMCC No.22345的杂交瘤细胞株OTI6A6和OTI3G6分泌的抗人S100B蛋白单克隆抗体OTI6A6和OTI3G6。
3.根据权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI6A6和鼠单克隆抗体OTI3G6,其特征在于:以真核细胞293T表达的S100B全长蛋白为免疫原,通过免疫注射小鼠,取免疫小鼠脾脏制备悬液,并使之与骨髓瘤细胞进行细胞融合获得。
4.根据权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI6A6,其特征在于:其轻链可变区(VL)含112aa,其氨基酸序列如SEQ ID NO.1所示;所述抗体的重链(VH)含116aa,其氨基酸序列如SEQ ID No.2所示。
5.根据权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI6A6,其中VL区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为27aa-38aa,56aa-58aa和95aa-101aa。其氨基酸序列分别如SEQ ID No.3-5所示。
6.根据权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI6A6,其中VH区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为26aa-35aa,53aa-62aa和101aa-105aa。其氨基酸残基序列分别如SEQ ID No.6-8所示。
7.根据权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI3G6,其特征在于:其轻链可变区(VL)含110aa,其氨基酸序列如SEQ ID NO.11所示;所述抗体的重链(VH)含113aa,其氨基酸序列如SEQ ID No.12所示。
8.根据权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI3G6,其中VL区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为27aa-37aa,55aa-57aa和94aa-99aa。其氨基酸序列分别如SEQ ID No.13-15所示。
9.根据权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI3G6,其中VH区域包括3个抗原决定簇:CDR1、CDR2和CDR3,抗原决定簇的区域相应地分别为26aa-34aa,52aa-58aa和97aa-102aa。其氨基酸残基序列分别如SEQ ID No.16-18所示。
10.权利要求2所述的抗人S100B蛋白鼠单克隆抗体OTI6A6和鼠单克隆抗体OTI3G6,可以识别人S100B蛋白表面的不同抗原决定簇。
11.权利要求2所述的抗人S100B蛋白鼠单克隆抗体的应用,其特征在于:用于人S100B蛋白免疫检测试剂盒的制备,包括但不限于双抗体夹心ELISA法和化学发光免疫检测试剂盒。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110828284.3A CN114181908B (zh) | 2021-07-23 | 2021-07-23 | 抗人s100b蛋白鼠单克隆抗体及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110828284.3A CN114181908B (zh) | 2021-07-23 | 2021-07-23 | 抗人s100b蛋白鼠单克隆抗体及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN114181908A true CN114181908A (zh) | 2022-03-15 |
CN114181908B CN114181908B (zh) | 2023-11-17 |
Family
ID=80600985
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110828284.3A Active CN114181908B (zh) | 2021-07-23 | 2021-07-23 | 抗人s100b蛋白鼠单克隆抗体及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114181908B (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115044560B (zh) * | 2022-06-23 | 2023-07-28 | 北京中杉金桥生物技术有限公司 | 一种杂交瘤细胞株、抗人erg蛋白单克隆抗体及其应用 |
CN114874319B (zh) * | 2022-06-27 | 2023-09-08 | 河南大学 | 一种用于急性肾损伤检测的cryab抗体及其应用 |
CN116874596A (zh) * | 2023-09-06 | 2023-10-13 | 南京佰抗生物科技有限公司 | 抗S100β蛋白的单克隆抗体及其制备方法和应用 |
CN116903739A (zh) * | 2023-03-28 | 2023-10-20 | 中国人民解放军军事科学院军事医学研究院 | 一种抗s100b蛋白的抗体及其应用 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101970487A (zh) * | 2007-11-01 | 2011-02-09 | 惠氏有限责任公司 | Gdf8抗体及其用途 |
WO2016149116A1 (en) * | 2015-03-13 | 2016-09-22 | University Of Maryland, Baltimore | Antibodies targeting s100b and methods of use |
CN108179149A (zh) * | 2018-01-03 | 2018-06-19 | 青岛瑞斯凯尔生物科技有限公司 | S100b突变体及其应用 |
CN111487407A (zh) * | 2019-01-28 | 2020-08-04 | 艾维可生物科技有限公司 | 一种s100b蛋白的检测试剂盒及其使用方法 |
CN111886247A (zh) * | 2018-01-05 | 2020-11-03 | Ac免疫有限公司 | 错折叠tdp-43结合分子 |
CN112961242A (zh) * | 2020-06-30 | 2021-06-15 | 广州百暨基因科技有限公司 | 抗b7h3抗体及其应用 |
-
2021
- 2021-07-23 CN CN202110828284.3A patent/CN114181908B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101970487A (zh) * | 2007-11-01 | 2011-02-09 | 惠氏有限责任公司 | Gdf8抗体及其用途 |
WO2016149116A1 (en) * | 2015-03-13 | 2016-09-22 | University Of Maryland, Baltimore | Antibodies targeting s100b and methods of use |
CN108179149A (zh) * | 2018-01-03 | 2018-06-19 | 青岛瑞斯凯尔生物科技有限公司 | S100b突变体及其应用 |
CN111886247A (zh) * | 2018-01-05 | 2020-11-03 | Ac免疫有限公司 | 错折叠tdp-43结合分子 |
CN111487407A (zh) * | 2019-01-28 | 2020-08-04 | 艾维可生物科技有限公司 | 一种s100b蛋白的检测试剂盒及其使用方法 |
CN112961242A (zh) * | 2020-06-30 | 2021-06-15 | 广州百暨基因科技有限公司 | 抗b7h3抗体及其应用 |
Non-Patent Citations (1)
Title |
---|
徐卫平等: "ELISA检测血清S100B蛋白方法的建立与临床应用", 上海免疫学杂志, no. 2002, pages 119 - 120 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115044560B (zh) * | 2022-06-23 | 2023-07-28 | 北京中杉金桥生物技术有限公司 | 一种杂交瘤细胞株、抗人erg蛋白单克隆抗体及其应用 |
CN114874319B (zh) * | 2022-06-27 | 2023-09-08 | 河南大学 | 一种用于急性肾损伤检测的cryab抗体及其应用 |
CN116903739A (zh) * | 2023-03-28 | 2023-10-20 | 中国人民解放军军事科学院军事医学研究院 | 一种抗s100b蛋白的抗体及其应用 |
CN116903739B (zh) * | 2023-03-28 | 2023-12-15 | 中国人民解放军军事科学院军事医学研究院 | 一种抗s100b蛋白的抗体及其应用 |
CN116874596A (zh) * | 2023-09-06 | 2023-10-13 | 南京佰抗生物科技有限公司 | 抗S100β蛋白的单克隆抗体及其制备方法和应用 |
CN116874596B (zh) * | 2023-09-06 | 2023-11-24 | 南京佰抗生物科技有限公司 | 抗S100β蛋白的单克隆抗体及其制备方法和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN114181908B (zh) | 2023-11-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110684740B (zh) | 一种抗人泛素羧基末端水解酶-1(uch-l1)的单克隆抗体及其应用 | |
CN114181908B (zh) | 抗人s100b蛋白鼠单克隆抗体及其应用 | |
CN112250763B (zh) | 靶向SARS-CoV-2冠状病毒的抗体及其诊断和检测用途 | |
CN110616192B (zh) | 一种抗人神经丝轻链(nefl)的单克隆抗体及其应用 | |
CN113683688B (zh) | 抗人类免疫缺陷病毒ⅰ型p24抗原(hiv-1 p24)兔单克隆抗体及其应用 | |
US10494430B2 (en) | Anti-active GIP antibody | |
KR102012123B1 (ko) | 뎅기 바이러스 및 지카 바이러스의 비구조단백질 1에 동시에 결합 가능한 항체를 생산하는 하이브리도마 및 이로부터 생성된 항체, 이의 용도 | |
KR102660061B1 (ko) | 항 열대열 말라리아 원충 hrp-ii 항체 | |
CN113817062B (zh) | 抗人羟基类固醇17-β脱氢酶13(HSD17B13)兔单克隆抗体及其应用 | |
CN113956355B (zh) | 抗人脑利钠肽(bnp)兔单克隆抗体及其应用 | |
CN110894236B (zh) | 一种抗曲霉菌半乳甘露聚糖的单克隆抗体及其应用 | |
KR20220055423A (ko) | SARS-CoV-2 특이적 신규 항체를 이용한 SARS-CoV-2의 검출방법 | |
CN113150139A (zh) | 一种PBP2a单克隆抗体及其制备方法和应用 | |
JP2002020399A (ja) | ノルウォークウイルス(nv)を認識するモノクローナル抗体 | |
CN111808191A (zh) | 一种用于检测血清中vegf含量的抗体对及其用途 | |
CN110702913A (zh) | 一种用于定量检测贝氏柯克斯体i相株的单抗组合物 | |
CN114456265B (zh) | 一种抗hfabp单克隆抗体及其应用 | |
CN107383200B (zh) | 鼠源抗人IgE单克隆抗体的制备方法及其应用 | |
CN117624355B (zh) | 一种抗人乙酰化tau274兔单克隆抗体及其应用 | |
CN111378035B (zh) | 抗恶性疟原虫hrp-ii重组抗体 | |
CN117024586B (zh) | 一种抗体组合物及应用于检测血液中ykl-40浓度的方法 | |
JP6099393B2 (ja) | オートタキシンアイソフォーム特異的抗体および検出方法 | |
CN113009139B (zh) | 检测猪伪狂犬病毒抗原的酶联免疫试剂盒及其应用 | |
CN108727493B (zh) | 抗Stathmin单克隆抗体及其用途 | |
CN117624354A (zh) | 一种抗人乙酰化tau281兔单克隆抗体及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |