CN114130066A - Separation device suitable for biological reagent development - Google Patents
Separation device suitable for biological reagent development Download PDFInfo
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- CN114130066A CN114130066A CN202111435559.3A CN202111435559A CN114130066A CN 114130066 A CN114130066 A CN 114130066A CN 202111435559 A CN202111435559 A CN 202111435559A CN 114130066 A CN114130066 A CN 114130066A
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- separation
- fixedly connected
- flow guide
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- 238000000926 separation method Methods 0.000 title claims abstract description 66
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 33
- 238000003756 stirring Methods 0.000 claims abstract description 24
- 230000000903 blocking effect Effects 0.000 claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000009434 installation Methods 0.000 claims abstract description 11
- 230000000149 penetrating effect Effects 0.000 claims abstract description 9
- 230000000712 assembly Effects 0.000 claims abstract description 8
- 238000000429 assembly Methods 0.000 claims abstract description 8
- 230000005540 biological transmission Effects 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 abstract description 2
- 235000017491 Bambusa tulda Nutrition 0.000 abstract description 2
- 241001330002 Bambuseae Species 0.000 abstract description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 abstract description 2
- 239000011425 bamboo Substances 0.000 abstract description 2
- 230000006872 improvement Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000010364 biochemical engineering Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/04—Breaking emulsions
- B01D17/041—Breaking emulsions with moving devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/12—Auxiliary equipment particularly adapted for use with liquid-separating apparatus, e.g. control circuits
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- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a separating device suitable for biological reagent development, which relates to the technical field of biological reagent development, and comprises a separating box and is characterized in that: the flow dividing cylinder is arranged in the separation box, and the bottom of the separation box is provided with an installation cylinder; the separation mechanism is arranged in the shunting cylinder and comprises a blocking block, the blocking block is slidably arranged in the shunting cylinder, two sides of the top of the shunting cylinder are fixedly connected with fixed blocks, an adjusting rod and an installing column are respectively arranged on the two fixed blocks in a penetrating manner, the adjusting rod is in threaded connection with the blocking block, the installing column is in sliding connection with the blocking block, stirring assemblies are arranged on two sides of the separation cylinder, and after the stirring assemblies stir and separate reagents, the separation box moves up and down to realize layered flow guide; the honeycomb duct below sets up the containing box, the installation section of thick bamboo lower extreme sets up piston assembly, and piston assembly drives the honeycomb duct and reciprocates, realizes dividing the liquid water conservancy diversion.
Description
Technical Field
The invention relates to the technical field of biological reagent development, in particular to a separation device suitable for biological reagent development.
Background
The biological engineering includes five major projects, namely genetic engineering, cell engineering, microbial fermentation engineering, enzyme biochemical engineering and bioreactor engineering, and in the five major fields, the two projects have the functions of taking conventional bacteria or animal and plant cell strains as specific genetic material receptors to carry out large-scale culture so as to fully exert the inherent potential of the conventional bacteria or animal and plant cell strains.
And traditional separator, when in actual use, will take the sample respectively according to the condition of liquid layering sometimes, traditional separator, generally all manual work carries out the layering to the sample respectively and draws, and the work efficiency of this kind of mode separation is lower, and when the separation, the condition that subsides appears in liquid easily, leads to partial reagent to mix, influences the purity of actual separation.
Disclosure of Invention
The present invention provides a separation device suitable for the development of biological reagents, solving the problems of the background art mentioned above.
In order to achieve the purpose, the invention provides the following technical scheme:
a separation device suitable for use in bioreagent development, comprising a separation chamber and further comprising:
the flow dividing cylinder is arranged in the separation box, and the bottom of the separation box is provided with an installation cylinder;
the separation mechanism is arranged in the shunting cylinder and comprises a blocking block, the blocking block is slidably arranged in the shunting cylinder, two sides of the top of the shunting cylinder are fixedly connected with fixed blocks, an adjusting rod and an installing column are respectively arranged on the two fixed blocks in a penetrating manner, the adjusting rod is in threaded connection with the blocking block, the installing column is in sliding connection with the blocking block, stirring assemblies are arranged on two sides of the separation cylinder, and after the stirring assemblies stir and separate reagents, the separation box moves up and down to realize layered flow guide;
the flow guide mechanism comprises a flow guide cylinder, the flow guide cylinder is arranged in the mounting cylinder in a sliding mode, a flow guide opening is formed in one side of the flow guide cylinder, the flow guide pipe is fixedly connected to one side of the mounting cylinder, the containing box is arranged below the flow guide pipe, a piston assembly is arranged at the lower end of the mounting cylinder and drives the flow guide cylinder to move up and down, and liquid separation and flow guide are achieved;
and the driving mechanism is arranged on one side of the mounting cylinder and is used for driving the separating mechanism and the flow guide mechanism.
As a preferred technical scheme of the invention, the stirring assembly comprises transmission columns, two sides of each transmission column are rotatably connected with the separation box, the separation box between the rotation columns is rotatably connected with the rotation columns, the rotation columns are fixedly connected with stirring blades, and the transmission columns are in transmission connection with the rotation columns.
As a preferable technical scheme of the invention, the piston assembly comprises a bearing rod, two sides of the bearing rod are fixedly connected with a supporting column, a guide column is fixedly connected on the bearing rod, and a piston plate is arranged on the guide column in a sliding and penetrating manner.
According to a preferable technical scheme of the invention, a piston column is arranged on the piston plate in a sliding and penetrating manner, the piston column is of a T-shaped structure, an elastic part is sleeved at one end of the piston column, the top of the piston column is fixedly connected with the guide cylinder, and a stop block is fixedly connected with the inner wall of the mounting cylinder.
As a preferred technical scheme of the invention, the driving mechanism comprises a fixed plate, a driving column is rotatably arranged on the fixed plate in a penetrating manner, one side of the driving column is fixedly connected with a driving wheel, one side of the driving wheel is fixedly connected with a fixed shaft, one end of the fixed shaft is hinged with a transmission rod, and the transmission rod is hinged with the piston plate.
As a preferable technical scheme of the invention, the side wall of the separation box above the driving column is fixedly connected with a driving motor, the driving motor is in transmission connection with the driving column, one side of the driving column is fixedly connected with a driving wheel, one side of the driving column is fixedly connected with a mounting wheel, and the mounting wheel is in transmission connection with the driving wheel.
As a preferred technical scheme of the invention, one side of the rotating column penetrates through the side wall of the separation box, the end part of the rotating column is fixedly connected with the rotating wheel, linkage wheels are fixedly sleeved on the transmission columns on the two sides of the separation box, and the linkage wheels are in transmission connection with the rotating wheels.
The invention has the following advantages: when the reagent separating device is used, reagents to be mixed are placed in a separating box, then a driving motor is started to drive a driving column to rotate, the driving column drives a rotating column in the separating box to rotate through a driving wheel, and further drives a stirring blade to fully mix and stir liquid until the liquid is actually mixed to generate layering, then a regulating rod is rotated to drive a blocking block to move downwards so as to enable a diversion hole at the uppermost layer of a diversion cylinder to be communicated, then a piston plate is driven to move upwards and downwards through a transmission rod under the action of a driving wheel, and further a diversion cylinder above the diversion cylinder is driven to move upwards and downwards through the piston column, when the diversion cylinder moves downwards, the diversion hole communicated with the uppermost layer is contacted with a first layer of reagents in the separating box, enters the diversion cylinder, then the diversion cylinder moves upwards, when the piston column is contacted with the blocking block, the blockage is realized, at the moment, the diversion cylinder is static and the diversion port is contacted with a diversion pipe, first layer liquid reagent flows into the containing box in, repeats above-mentioned step, is accomodate when first layer liquid and accomplishes the back, continues to rotate the regulation pole and drives the sprue and move down for second floor water conservancy diversion hole is opened, then carries out collecting of second floor liquid, and the device's separation mode is overall, and the separation precision is high, and the great improvement of the separation efficiency of device, and the practicality is better.
Drawings
FIG. 1 is a schematic front view of a separation apparatus suitable for development of a biological reagent.
FIG. 2 is a schematic side view of a separation apparatus suitable for use in the development of biological reagents.
FIG. 3 is a schematic front view of a separation apparatus suitable for biological reagent development.
FIG. 4 is a schematic side view of a separation device suitable for biological reagent development.
FIG. 5 is a schematic top view of a separation device suitable for use in the development of biological reagents.
In the figure: 1. a separation tank; 2. a shunt cylinder; 3. stirring blades; 4. a drive post; 5. a draft tube; 6. a driving wheel; 7. a fixed shaft; 8. a transmission rod; 9. a piston plate; 10. mounting the cylinder; 11. a flow guide pipe; 12. an elastic member; 13. a storage box; 14. a support pillar; 15. a piston post; 16. a guide post; 17. a carrier bar; 18. a linkage wheel; 19. a rotating wheel; 20. mounting wheels; 21. a drive motor; 22. a drive wheel; 23. a fixing plate; 24. a flow guide hole; 25. a fixed block; 26. adjusting a rod; 27. blocking; 28. a port; 29. mounting a column; 30. rotating the column; 31. a flow guide port; 32. a stopper; 33. a drive column; 34. a separating mechanism; 35. a flow guide mechanism; 36. a drive mechanism; 37. a piston assembly; 38. and a stirring component.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.
Example 1
Referring to fig. 1-5, a separation device suitable for biological reagent development comprises a separation box 1, and further comprises:
the flow distributing cylinder 2 is arranged in the separation box 1, and the bottom of the separation box 1 is fixedly provided with an installation cylinder 10;
the separating mechanism 34 is arranged in the shunting cylinder 2 and comprises a blocking block 27, a through hole 28 is formed in the blocking block 27, the blocking block 27 is arranged in the shunting cylinder 2 in a sliding mode, the two sides of the top of the shunting cylinder 2 are fixedly connected with fixing blocks 25, an adjusting rod 26 and an installing column 29 are arranged on the two fixing blocks 25 in a penetrating mode respectively, the adjusting rod 26 is rotatably connected with the fixing blocks 25, the adjusting rod 26 is in threaded connection with the blocking block 27, the installing column 29 is in sliding connection with the blocking block 27, stirring assemblies 38 are arranged on the two sides of the separating cylinder, and after the stirring assemblies 38 stir and separate reagents, the separating box 1 moves up and down to realize layered flow guiding;
the flow guide mechanism 35 is arranged below the flow distribution cylinder 2 and comprises a flow guide cylinder 5, the flow guide cylinder 5 is arranged in the installation cylinder 10 in a sliding mode, a flow guide opening 31 is formed in one side of the flow guide cylinder 5, the top of the flow guide cylinder 5 is fixedly connected with the flow distribution cylinder 2, a flow guide pipe 11 is fixedly connected to one side of the installation cylinder 10, an accommodating box 13 is arranged below the flow guide pipe 11, a piston assembly 37 is arranged at the lower end of the installation cylinder 10, and the piston assembly 37 drives the flow guide cylinder 5 to move up and down to realize liquid separation and flow guide;
the driving mechanism 36 arranged on one side of the mounting cylinder 10 is used for driving the separating mechanism 34 and the flow guide mechanism 35, the separation is that the first layer of liquid reagent flows into the containing box 13 first, the steps are repeated, after the first layer of liquid is contained, the adjusting rod 26 continues to rotate to drive the blocking block 27 to move downwards, so that the second layer of flow guide holes 24 are opened, and then the second layer of liquid is collected until all the layered liquid is collected.
Example 2
Referring to fig. 1 to 5, the other contents of the present embodiment are the same as embodiment 1, except that: the stirring assembly 38 comprises a transmission column 4, two sides of the transmission column 4 are rotatably connected with the separation box 1, the separation box 1 between the rotation columns 30 is rotatably connected with the rotation columns 30, the stirring blades 3 are fixedly connected on the rotation columns 30, and the transmission column 4 is in transmission connection with the rotation columns 30.
The piston assembly 37 comprises a bearing rod 17, two sides of the bearing rod 17 are fixedly connected with the supporting columns 14, the bearing rod 17 is fixedly connected with the guide columns 16, and the guide columns 16 are provided with piston plates 9 in a sliding penetrating mode.
Sliding on the piston plate 9 and running through and setting up piston post 15, piston post 15 is T shape structure, elastic component 12 is established to 15 pot heads of piston post, 15 top and draft tube 5 fixed connection of piston post, installation section of thick bamboo 10 inner wall fixed connection dog 32.
The driving mechanism 36 comprises a fixing plate 23, the fixing plate 23 is fixedly connected with the side wall of the separation box 1, a driving column 33 is arranged on the fixing plate 23 in a rotating and penetrating mode, a driving wheel 6 is fixedly connected to one side of the driving column 33, a fixing shaft 7 is fixedly connected to one side of the driving wheel 6, one end of the fixing shaft 7 is hinged to a transmission rod 8, and the transmission rod 8 is hinged to a piston plate 9.
The separation box 1 lateral wall fixed connection driving motor 21 of drive post 33 top is connected through belt transmission between driving motor 21 and the drive post 33, drive post 33 one side fixed connection drive wheel 22, 4 one side fixed connection installation wheels 20 of transmission post are connected through belt transmission between installation wheels 20 and the drive wheel 22.
One side of the rotating column 30 penetrates through the side wall of the separation box 1, the end part of the rotating column is fixedly connected with a rotating wheel 19, linkage wheels 18 are fixedly sleeved on the transmission columns 4 on the two sides of the separation box 1, and the linkage wheels 18 are in transmission connection with the rotating wheel 19 through belts.
In the implementation process of the invention, when the reagent separating device is used, reagents to be mixed are placed in a separating box 1, then a driving motor 21 is started to drive a driving column 33 to rotate, the driving column 33 drives a rotating column 30 in the separating box 1 to rotate through a driving wheel 22, further drives a stirring blade 3 to fully mix and stir liquid until the liquid is layered actually mixed, then a regulating rod 26 is rotated to drive a blocking block 27 to move downwards so as to enable a diversion hole 24 at the uppermost layer of a diversion cylinder 2 to be communicated, then a piston plate 9 is driven to move upwards and downwards through a driving rod 8 under the action of a driving wheel 6, further drives the diversion cylinder 2 above the diversion cylinder 5 to move upwards and downwards through a piston column 15, when the diversion cylinder 2 moves downwards, the diversion hole 24 communicated at the uppermost layer is contacted with a first layer of reagents in the separating box 1, enters the diversion cylinder 2, then enters the diversion cylinder 5, then the diversion cylinder 2 moves upwards, when piston post 15 and dog 32 contact, realize the card and die, the draft tube 5 is static different this moment, and the water conservancy diversion mouth 31 corresponds with the contact of honeycomb duct 11, first layer liquid reagent flows into in the containing box 13, repeat above-mentioned step, after first layer liquid is accomodate the completion, it moves down to continue to rotate regulation pole 26 and drive sprue 27, make second floor water conservancy diversion hole 24 open, then collect of second floor liquid, the device's separation mode is overall, the separation precision is high, and the great improvement of the separation efficiency of device, the practicality is better.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A separator suitable for biological reagent development, includes the separator box, its characterized in that still includes:
the flow dividing cylinder is arranged in the separation box, and the bottom of the separation box is provided with an installation cylinder;
the separation mechanism is arranged in the shunting cylinder and comprises a blocking block, the blocking block is slidably arranged in the shunting cylinder, two sides of the top of the shunting cylinder are fixedly connected with fixed blocks, an adjusting rod and an installing column are respectively arranged on the two fixed blocks in a penetrating manner, the adjusting rod is in threaded connection with the blocking block, the installing column is in sliding connection with the blocking block, stirring assemblies are arranged on two sides of the separation cylinder, and after the stirring assemblies stir and separate reagents, the separation box moves up and down to realize layered flow guide;
the flow guide mechanism comprises a flow guide cylinder, the flow guide cylinder is arranged in the mounting cylinder in a sliding mode, a flow guide opening is formed in one side of the flow guide cylinder, the flow guide pipe is fixedly connected to one side of the mounting cylinder, the containing box is arranged below the flow guide pipe, a piston assembly is arranged at the lower end of the mounting cylinder and drives the flow guide cylinder to move up and down, and liquid separation and flow guide are achieved;
and the driving mechanism is arranged on one side of the mounting cylinder and is used for driving the separating mechanism and the flow guide mechanism.
2. The separation device suitable for biological reagent development according to claim 1, wherein the stirring assembly comprises transmission columns, two sides of each transmission column are rotatably connected with the separation box, the separation box between the transmission columns is rotatably connected with the rotation columns, stirring blades are fixedly connected onto the rotation columns, and the transmission columns are in transmission connection with the rotation columns.
3. The separation device suitable for biological reagent development according to claim 1, wherein the piston assembly comprises a carrying rod, two sides of the carrying rod are fixedly connected with the support column, the carrying rod is fixedly connected with the guide column, and the guide column is slidably provided with the piston plate.
4. The separation device suitable for biological reagent development according to claim 3, wherein a piston column is slidably disposed through the piston plate, the piston column is of a T-shaped structure, an elastic member is sleeved on one end of the piston column, the top of the piston column is fixedly connected with the guide cylinder, and a stopper is fixedly connected to the inner wall of the mounting cylinder.
5. The separation device for bioreagent development according to claim 1, wherein the driving mechanism comprises a fixed plate, a driving column is rotatably disposed on the fixed plate, a driving wheel is fixedly connected to one side of the driving column, a fixed shaft is fixedly connected to one side of the driving wheel, one end of the fixed shaft is hinged to a transmission rod, and the transmission rod is hinged to the piston plate.
6. The separation device suitable for biological reagent development according to claim 5, wherein the side wall of the separation box above the driving column is fixedly connected with a driving motor, the driving motor is in transmission connection with the driving column, one side of the driving column is fixedly connected with a driving wheel, one side of the driving column is fixedly connected with a mounting wheel, and the mounting wheel is in transmission connection with the driving wheel.
7. The separation device suitable for biological reagent development as claimed in claim 6, wherein one side of the rotation column passes through the side wall of the separation box, and the end part of the rotation column is fixedly connected with a rotation wheel, linkage wheels are fixedly sleeved on the transmission columns at two sides of the separation box, and the linkage wheels are in transmission connection with the rotation wheels.
Priority Applications (1)
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CN202111435559.3A CN114130066A (en) | 2021-11-29 | 2021-11-29 | Separation device suitable for biological reagent development |
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CN202111435559.3A CN114130066A (en) | 2021-11-29 | 2021-11-29 | Separation device suitable for biological reagent development |
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107790033A (en) * | 2017-12-02 | 2018-03-13 | 阳新县泰丰植保专业合作社 | A kind of plant protection medicament mixing device |
CN210814285U (en) * | 2019-10-18 | 2020-06-23 | 安徽天邦生物技术有限公司 | Solid-liquid separation device suitable for biological fermentation engineering |
CN111919719A (en) * | 2020-08-21 | 2020-11-13 | 丁乙飞 | Intelligent rotary water-saving irrigation system and working method |
CN111974267A (en) * | 2020-08-27 | 2020-11-24 | 董乾坤 | Agitating unit is used in industrial chemicals processing convenient to wash |
CN112374650A (en) * | 2020-11-16 | 2021-02-19 | 瑞安市智造科技有限公司 | A diverging device for large-scale coating machine |
CN112403329A (en) * | 2020-11-17 | 2021-02-26 | 卞雪丹 | Agricultural fertilizer agitating unit |
CN112755852A (en) * | 2021-01-29 | 2021-05-07 | 浙江恒达新材料股份有限公司 | A diversified rabbling mechanism of many fibre magma intelligence for dialyzing function paper |
CN213555488U (en) * | 2020-09-24 | 2021-06-29 | 江西五麟祥生物科技有限公司 | Plant stock solution extraction device |
CN113600349A (en) * | 2021-07-26 | 2021-11-05 | 淄博威世能净油设备有限公司 | Automatic oil-collecting centrifugal oil purifier and use method thereof |
-
2021
- 2021-11-29 CN CN202111435559.3A patent/CN114130066A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107790033A (en) * | 2017-12-02 | 2018-03-13 | 阳新县泰丰植保专业合作社 | A kind of plant protection medicament mixing device |
CN210814285U (en) * | 2019-10-18 | 2020-06-23 | 安徽天邦生物技术有限公司 | Solid-liquid separation device suitable for biological fermentation engineering |
CN111919719A (en) * | 2020-08-21 | 2020-11-13 | 丁乙飞 | Intelligent rotary water-saving irrigation system and working method |
CN111974267A (en) * | 2020-08-27 | 2020-11-24 | 董乾坤 | Agitating unit is used in industrial chemicals processing convenient to wash |
CN213555488U (en) * | 2020-09-24 | 2021-06-29 | 江西五麟祥生物科技有限公司 | Plant stock solution extraction device |
CN112374650A (en) * | 2020-11-16 | 2021-02-19 | 瑞安市智造科技有限公司 | A diverging device for large-scale coating machine |
CN112403329A (en) * | 2020-11-17 | 2021-02-26 | 卞雪丹 | Agricultural fertilizer agitating unit |
CN112755852A (en) * | 2021-01-29 | 2021-05-07 | 浙江恒达新材料股份有限公司 | A diversified rabbling mechanism of many fibre magma intelligence for dialyzing function paper |
CN113600349A (en) * | 2021-07-26 | 2021-11-05 | 淄博威世能净油设备有限公司 | Automatic oil-collecting centrifugal oil purifier and use method thereof |
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