CN214881491U - Device for extracting supernatant in microcarrier cell culture and bioreactor - Google Patents
Device for extracting supernatant in microcarrier cell culture and bioreactor Download PDFInfo
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- CN214881491U CN214881491U CN202120978003.8U CN202120978003U CN214881491U CN 214881491 U CN214881491 U CN 214881491U CN 202120978003 U CN202120978003 U CN 202120978003U CN 214881491 U CN214881491 U CN 214881491U
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Abstract
The utility model discloses a device for extracting supernatant in microcarrier cell culture, which comprises a vertically arranged stirring shaft, a filter, a stirring paddle and a pipette, wherein the filter, the stirring paddle and the pipette are arranged on the stirring shaft; the filter is cylindrical and has an opening at the upper end, the stirring shaft is inserted into the filter, and the filter is connected to the lower part of the stirring shaft; the stirring paddle is arranged on the outer side of the filter. The utility model also provides a bioreactor, including jar body, top cap and be used for the device of microcarrier cell culture extraction supernatant. By adopting the technical scheme, the device for extracting the supernatant from the microcarrier cell culture is arranged, so that the liquid at the lower position in the tank body can be extracted by the pipette, and the problem that only a small part of liquid in a bioreactor can be extracted in the prior art is effectively solved.
Description
Technical Field
The utility model relates to a device and bioreactor that is used for microcarrier cell culture extraction supernatant belongs to cell biological reaction instrument equipment field.
Background
The Bioreactor (Bioreactor) is an appropriate environment or engineering equipment for providing biochemical reactions, and generally means an apparatus having a function of simulating organisms by using enzymes or organisms (microorganisms), and can perform biochemical reactions outside cells, and can perform both aerobic and anaerobic reactions during the simulation. The source of the bioreactor can be divided into a natural bioreactor, an engineering bioreactor and the like, the bioreactor is usually cylindrical, the volume of the bioreactor is from several liters to several cubic meters, and the bioreactor is usually made of stainless steel. The feeding method can be divided into Batch reactor (Batch reactor), fed Batch reactor (fed Batch), and Continuous flow reactor (Continuous reactor), such as Continuous stirred tank reactor, chemostat, etc.
The microorganism growing in the bioreactor can be soaked in a liquid medium or attached to the surface of a solid medium, the supernatant in the bioreactor can be extracted by a pipette, and since the stirring is required to be rotated and filtered during liquid extraction in the stirring type reactor, the filtering component is arranged at the upper part of the rotating shaft and is positioned above the stirring paddle, only a small part of liquid in the bioreactor can be extracted.
SUMMERY OF THE UTILITY MODEL
Therefore, the utility model aims at providing a can realize extracting device and bioreactor that are used for microcarrier cell culture extraction supernatant of most liquid in the reactor.
In order to achieve the purpose, the device for extracting the supernatant for microcarrier cell culture comprises a vertically arranged stirring shaft, a filter, a stirring paddle and a pipette, wherein the filter, the stirring paddle and the pipette are arranged on the stirring shaft; the filter is cylindrical and has an opening at the upper end, the stirring shaft is inserted into the filter, and the filter is connected to the lower part of the stirring shaft; the outer side of the filter is provided with the stirring paddle; a pipette is inserted down into the filter interior at the upper end of the filter.
The stirring rake includes flabellum and collar, the flabellum set up in the outside of collar, the collar is in the outside of filter is fixed through the screw.
The stirring rake is for setting up in a plurality of on the filter.
The stirring paddles are uniformly distributed in the axial direction of the filter.
The bottom of filter is provided with the mount pad, the lower extreme of (mixing) shaft pass through the screw install in on the mount pad.
The side wall of the filter is provided with filter holes, the device for extracting the supernatant from the microcarrier cell culture comprises a plurality of filters with different filter hole pore sizes, and the filters can be detachably arranged on the stirring shaft, so that the filters can be used in a replaceable way.
The utility model also provides a bioreactor, including a jar body, top cap and as before be used for microcarrier cell culture to extract the device of supernatant be provided with the bearing frame on the top cap, (mixing) shaft pass through the bearing install in on the bearing frame, the pipette is worn out pass through silica gel hose connection in peristaltic pump behind the top cap.
The pipette comprises a lower vertical section inserted into the filter, a bending section connected to the vertical section and an upper vertical section connected to the bending section; the lower vertical section is inserted into the inside bottom of the filter.
A bolt is arranged on the upper vertical section, an axial through hole is formed in the bolt, and the vertical section penetrates through the through hole; the bolt penetrates through the mounting hole in the top cover and then is screwed on a nut.
The lower end of the filter is positioned at a position close to the bottom of the tank body.
By adopting the technical scheme, the utility model discloses a device and bioreactor for microcarrier cell culture extraction supernatant sets up the stirring rake in the outside of the filter of tube-shape, and fixes the filter suit in the outside of (mixing) shaft, consequently when driving the (mixing) shaft through the motor and rotate, can drive the filter and rotate synchronously, thereby drive the stirring rake and rotate, reach the purpose of stirring in the reactor, simultaneously, insert the pipette in the top opening of filter is down to the filter in, consequently can not cause the hindrance when the filter rotates, and the liquid after passing through the filter filtration can be taken out by the pipette; due to the arrangement of the device for extracting the supernatant for microcarrier cell culture, liquid at a lower position in the tank body can be extracted through the pipette, and the problem that only a small part of liquid in the bioreactor can be extracted in the prior art is effectively solved.
Drawings
FIG. 1 is a schematic structural diagram of a bioreactor of the present invention.
Fig. 2 is an exploded view of fig. 1.
FIG. 3 is a schematic view of the assembly structure of the filter, the stirring paddle and the stirring shaft.
Fig. 4 is an exploded view of fig. 3.
Fig. 5 is an exploded view of the paddle and filter.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
As shown in figures 1 and 2, the utility model provides a bioreactor, which comprises a transparent tank body 1, a top cover 2 and a device for extracting supernatant from microcarrier cell culture.
The device for extracting the supernatant from the microcarrier cell culture comprises a vertically arranged stirring shaft 3, a filter 4, a stirring paddle 5 and a pipette 6 which are arranged on the stirring shaft 3, wherein a bearing seat 7 is arranged on the top cover 2, the stirring shaft 3 is arranged on the bearing seat 7 through a bearing, and the upper end of the stirring shaft 3 is used for connecting a power output end of a motor.
As shown in fig. 3 and 4, the filter 4 is cylindrical and has an open upper end, and the stirring shaft 3 is inserted into the filter 4. The bottom of the filter 4 is provided with a mounting seat 61, and the lower end of the stirring shaft 3 is mounted on the mounting seat 61 through a screw, so that the filter 4 and the stirring shaft 3 can synchronously rotate.
A plurality of paddles 5 are installed at the outer side of the filter 4, and the plurality of paddles 5 are uniformly distributed in the axial direction of the filter 4.
As shown in fig. 5, the stirring paddle 5 includes a fan blade 51 and a mounting ring 52, the fan blade 51 is disposed on the outer side of the mounting ring 52, and the mounting ring 52 is sleeved on the outer side of the filter 4 and fixed by screws.
The pipette 6 is inserted down inside the filter 4 at the upper end of the filter 4. The pipette 6 comprises a lower vertical section 61 inserted into the filter 4, an inflection section 62 connected to the vertical section, and an upper vertical section 63 connected to the inflection section 62; the lower vertical section 61 is inserted into the bottom of the inside of the filter 4, so that the liquid in the bottom of the inside of the filter 4 can be extracted.
A bolt 8 is arranged on the upper vertical section 63, an axial through hole is formed in the bolt 8, and the vertical section penetrates through the through hole; the bolt 8 is screwed on a nut 9 after passing through the mounting hole on the top cover 2.
Go up vertical section 63 and wear out connect in the peristaltic pump through the silica gel hose after top cap 2.
The side wall of the filter 4 is provided with filter holes, the device for extracting the supernatant from the microcarrier cell culture comprises a plurality of filters 4 with different filter hole pore sizes, and the filters 4 can be detachably arranged on a stirring shaft 3 so that the filters 4 can be replaced and used.
The lower end of the filter 4 is positioned close to the bottom of the tank body 1.
By adopting the technical scheme, the utility model discloses a device and bioreactor for microcarrier cell culture extraction supernatant, set up stirring rake 5 in the outside of the filter 4 of tube-shape, and fix filter 4 suit in the outside of (mixing) shaft 3, therefore when drive (mixing) shaft 3 through the motor and rotate, can drive filter 4 and rotate synchronously, thereby drive stirring rake 5 and rotate, reach the purpose of stirring in the reactor, meanwhile, insert pipette 6 in the top opening of filter 4 is in filter 4 is inside, therefore can not cause the hindrance when filter 4 rotates, and the liquid after through filter 4 filters can be taken out by pipette 6; due to the arrangement of the device for extracting the supernatant for microcarrier cell culture, the liquid at the lower position in the tank body 1 can be extracted through the pipette 6, and the problem that only a small part of liquid in a bioreactor can be extracted in the prior art is effectively solved.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.
Claims (10)
1. An apparatus for extracting supernatant for microcarrier cell culture, comprising: the stirring device comprises a stirring shaft, a filter, a stirring paddle and a pipette, wherein the stirring shaft is vertically arranged, and the filter, the stirring paddle and the pipette are arranged on the stirring shaft; the filter is cylindrical and has an opening at the upper end, the stirring shaft is inserted into the filter, and the filter is connected to the lower part of the stirring shaft; the outer side of the filter is provided with the stirring paddle; a pipette is inserted down into the filter interior at the upper end of the filter.
2. The device for extracting supernatant for microcarrier cell culture according to claim 1, wherein: the stirring rake includes flabellum and collar, the flabellum set up in the outside of collar, the collar is in the outside of filter is fixed through the screw.
3. The device for extracting supernatant for microcarrier cell culture according to claim 1, wherein: the stirring rake is for setting up in a plurality of on the filter.
4. The device for extracting supernatant for microcarrier cell culture according to claim 3, wherein: the stirring paddles are uniformly distributed in the axial direction of the filter.
5. The device for extracting supernatant for microcarrier cell culture according to any one of claims 1 to 4, wherein: the bottom of filter is provided with the mount pad, the lower extreme of (mixing) shaft pass through the screw install in on the mount pad.
6. The device for extracting supernatant for microcarrier cell culture according to any one of claims 1 to 4, wherein: the side wall of the filter is provided with filter holes, the device for extracting the supernatant from the microcarrier cell culture comprises a plurality of filters with different filter hole pore sizes, and the filters can be detachably arranged on the stirring shaft, so that the filters can be used in a replaceable way.
7. A bioreactor, characterized by: the device for extracting the supernatant from the microcarrier cell culture comprises a tank body, a top cover and the device for extracting the supernatant from the microcarrier cell culture according to any one of claims 1 to 6, wherein a bearing seat is arranged on the top cover, a stirring shaft is arranged on the bearing seat through a bearing, and the pipette penetrates out of the top cover and is connected to a peristaltic pump through a silica gel hose.
8. The bioreactor of claim 7, wherein: the pipette comprises a lower vertical section inserted into the filter, a bending section connected to the vertical section and an upper vertical section connected to the bending section; the lower vertical section is inserted into the inside bottom of the filter.
9. The bioreactor of claim 7, wherein: a bolt is arranged on the upper vertical section, an axial through hole is formed in the bolt, and the vertical section penetrates through the through hole; the bolt penetrates through the mounting hole in the top cover and then is screwed on a nut.
10. The bioreactor of any one of claims 7 to 9, wherein: the lower end of the filter is positioned at a position close to the bottom of the tank body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120978003.8U CN214881491U (en) | 2021-05-08 | 2021-05-08 | Device for extracting supernatant in microcarrier cell culture and bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120978003.8U CN214881491U (en) | 2021-05-08 | 2021-05-08 | Device for extracting supernatant in microcarrier cell culture and bioreactor |
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| CN214881491U true CN214881491U (en) | 2021-11-26 |
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| CN202120978003.8U Active CN214881491U (en) | 2021-05-08 | 2021-05-08 | Device for extracting supernatant in microcarrier cell culture and bioreactor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115895887A (en) * | 2022-12-13 | 2023-04-04 | 成都康华生物制品股份有限公司 | Influenza vaccine production device and method and influenza vaccine |
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2021
- 2021-05-08 CN CN202120978003.8U patent/CN214881491U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115895887A (en) * | 2022-12-13 | 2023-04-04 | 成都康华生物制品股份有限公司 | Influenza vaccine production device and method and influenza vaccine |
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