CN113122437A - Device for extracting supernatant in microcarrier cell culture and bioreactor - Google Patents

Device for extracting supernatant in microcarrier cell culture and bioreactor Download PDF

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Publication number
CN113122437A
CN113122437A CN202110501031.5A CN202110501031A CN113122437A CN 113122437 A CN113122437 A CN 113122437A CN 202110501031 A CN202110501031 A CN 202110501031A CN 113122437 A CN113122437 A CN 113122437A
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China
Prior art keywords
filter
cell culture
stirring
supernatant
extracting
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Pending
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CN202110501031.5A
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Chinese (zh)
Inventor
刘伟
鄢晓君
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Beijing Huakan Biotechnology Co ltd
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Beijing Huakan Biotechnology Co ltd
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Priority to CN202110501031.5A priority Critical patent/CN113122437A/en
Publication of CN113122437A publication Critical patent/CN113122437A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a device for extracting supernatant in microcarrier cell culture, which comprises a vertically arranged stirring shaft, a filter, a stirring paddle and a pipette, wherein the filter, the stirring paddle and the pipette are arranged on the stirring shaft; the filter is cylindrical and has an opening at the upper end, the stirring shaft is inserted into the filter, and the filter is connected to the lower part of the stirring shaft; the outer side of the filter is provided with the stirring paddle; a pipette is inserted down into the filter interior at the upper end of the filter. The invention also provides a bioreactor, which comprises a tank body, a top cover and a device for extracting supernatant from microcarrier cell culture. By adopting the technical scheme, the device for extracting the supernatant from the microcarrier cell culture is arranged, so that the liquid at the lower position in the tank body can be extracted by the pipette, and the problem that only a small part of liquid in a bioreactor can be extracted in the prior art is effectively solved.

Description

Device for extracting supernatant in microcarrier cell culture and bioreactor
Technical Field
The invention relates to a device for extracting supernatant in microcarrier cell culture and a bioreactor, belonging to the field of cell biological reaction instruments.
Background
The Bioreactor (Bioreactor) is an appropriate environment or engineering equipment for providing biochemical reactions, and generally means an apparatus having a function of simulating organisms by using enzymes or organisms (microorganisms), and can perform biochemical reactions outside cells, and can perform both aerobic and anaerobic reactions during the simulation. The source of the bioreactor can be divided into a natural bioreactor, an engineering bioreactor and the like, the bioreactor is usually cylindrical, the volume of the bioreactor is from several liters to several cubic meters, and the bioreactor is usually made of stainless steel. The feeding method can be divided into Batch reactor (Batch reactor), fed Batch reactor (fed Batch), and Continuous flow reactor (Continuous reactor), such as Continuous stirred tank reactor, chemostat, etc.
The microorganism growing in the bioreactor can be soaked in a liquid medium or attached to the surface of a solid medium, the supernatant in the bioreactor can be extracted by a pipette, and since the stirring is required to be rotated and filtered during liquid extraction in the stirring type reactor, the filtering component is arranged at the upper part of the rotating shaft and is positioned above the stirring paddle, only a small part of liquid in the bioreactor can be extracted.
Disclosure of Invention
Accordingly, an object of the present invention is to provide a device for extracting supernatant of microcarrier cell culture and a bioreactor, which can extract most of the liquid in the reactor.
In order to achieve the purpose, the device for extracting the supernatant for microcarrier cell culture comprises a vertically arranged stirring shaft, a filter, a stirring paddle and a pipette, wherein the filter, the stirring paddle and the pipette are arranged on the stirring shaft; the filter is cylindrical and has an opening at the upper end, the stirring shaft is inserted into the filter, and the filter is connected to the lower part of the stirring shaft; the outer side of the filter is provided with the stirring paddle; a pipette is inserted down into the filter interior at the upper end of the filter.
The stirring rake includes flabellum and collar, the flabellum set up in the outside of collar, the collar is in the outside of filter is fixed through the screw.
The stirring rake is for setting up in a plurality of on the filter.
The stirring paddles are uniformly distributed in the axial direction of the filter.
The bottom of filter is provided with the mount pad, the lower extreme of (mixing) shaft pass through the screw install in on the mount pad.
The side wall of the filter is provided with filter holes, the device for extracting the supernatant from the microcarrier cell culture comprises a plurality of filters with different filter hole pore sizes, and the filters can be detachably arranged on the stirring shaft, so that the filters can be used in a replaceable way.
The invention also provides a bioreactor which comprises a tank body, a top cover and the device for extracting the supernatant from the microcarrier cell culture, wherein the top cover is provided with a bearing seat, a stirring shaft is arranged on the bearing seat through a bearing, and a pipette penetrates out of the top cover and is connected to a peristaltic pump through a silica gel hose.
The pipette comprises a lower vertical section inserted into the filter, a bending section connected to the vertical section and an upper vertical section connected to the bending section; the lower vertical section is inserted into the inside bottom of the filter.
A bolt is arranged on the upper vertical section, an axial through hole is formed in the bolt, and the vertical section penetrates through the through hole; the bolt penetrates through the mounting hole in the top cover and then is screwed on a nut.
The lower end of the filter is positioned at a position close to the bottom of the tank body.
By adopting the technical scheme, the device for extracting the supernatant for microcarrier cell culture and the bioreactor are provided, the stirring paddle is arranged on the outer side of the cylindrical filter, and the filter is sleeved and fixed on the outer side of the stirring shaft, so that when the stirring shaft is driven by the motor to rotate, the filter can be driven to synchronously rotate, the stirring paddle is driven to rotate, the purpose of stirring in the reactor is achieved, meanwhile, the pipette is downwards inserted into the filter from the top opening of the filter, so that the filter cannot be blocked when rotating, and the liquid filtered by the filter can be extracted by the pipette; due to the arrangement of the device for extracting the supernatant for microcarrier cell culture, liquid at a lower position in the tank body can be extracted through the pipette, and the problem that only a small part of liquid in the bioreactor can be extracted in the prior art is effectively solved.
Drawings
FIG. 1 is a schematic view of the structure of a bioreactor according to the present invention.
Fig. 2 is an exploded view of fig. 1.
FIG. 3 is a schematic view of the assembly structure of the filter, the stirring paddle and the stirring shaft.
Fig. 4 is an exploded view of fig. 3.
Fig. 5 is an exploded view of the paddle and filter.
Detailed Description
The invention is described in further detail below with reference to the figures and the detailed description.
As shown in FIGS. 1 and 2, the present invention provides a bioreactor comprising a transparent tank 1, a top cover 2 and a device for extracting supernatant from microcarrier cell culture.
The device for extracting the supernatant from the microcarrier cell culture comprises a vertically arranged stirring shaft 3, a filter 4, a stirring paddle 5 and a pipette 6 which are arranged on the stirring shaft 3, wherein a bearing seat 7 is arranged on the top cover 2, the stirring shaft 3 is arranged on the bearing seat 7 through a bearing, and the upper end of the stirring shaft 3 is used for connecting a power output end of a motor.
As shown in fig. 3 and 4, the filter 4 is cylindrical and has an open upper end, and the stirring shaft 3 is inserted into the filter 4. The bottom of the filter 4 is provided with a mounting seat 61, and the lower end of the stirring shaft 3 is mounted on the mounting seat 61 through a screw, so that the filter 4 and the stirring shaft 3 can synchronously rotate.
A plurality of paddles 5 are installed at the outer side of the filter 4, and the plurality of paddles 5 are uniformly distributed in the axial direction of the filter 4.
As shown in fig. 5, the stirring paddle 5 includes a fan blade 51 and a mounting ring 52, the fan blade 51 is disposed on the outer side of the mounting ring 52, and the mounting ring 52 is sleeved on the outer side of the filter 4 and fixed by screws.
The pipette 6 is inserted down inside the filter 4 at the upper end of the filter 4. The pipette 6 comprises a lower vertical section 61 inserted into the filter 4, an inflection section 62 connected to the vertical section, and an upper vertical section 63 connected to the inflection section 62; the lower vertical section 61 is inserted into the bottom of the inside of the filter 4, so that the liquid in the bottom of the inside of the filter 4 can be extracted.
A bolt 8 is arranged on the upper vertical section 63, an axial through hole is formed in the bolt 8, and the vertical section penetrates through the through hole; the bolt 8 is screwed on a nut 9 after passing through the mounting hole on the top cover 2.
Go up vertical section 63 and wear out connect in the peristaltic pump through the silica gel hose after top cap 2.
The side wall of the filter 4 is provided with filter holes, the device for extracting the supernatant from the microcarrier cell culture comprises a plurality of filters 4 with different filter hole pore sizes, and the filters 4 can be detachably arranged on a stirring shaft 3 so that the filters 4 can be replaced and used.
The lower end of the filter 4 is positioned close to the bottom of the tank body 1.
By adopting the technical scheme, the device for extracting supernatant from microcarrier cell culture and the bioreactor provided by the invention have the advantages that the stirring paddle 5 is arranged on the outer side of the cylindrical filter 4, and the filter 4 is sleeved and fixed on the outer side of the stirring shaft 3, so that when the stirring shaft 3 is driven by a motor to rotate, the filter 4 can be driven to synchronously rotate, the stirring paddle 5 is driven to rotate, the purpose of stirring in the reactor is achieved, meanwhile, the pipette 6 is downwards inserted into the filter 4 from the top opening of the filter 4, so that no obstruction is caused when the filter 4 rotates, and liquid filtered by the filter 4 can be extracted by the pipette 6; due to the arrangement of the device for extracting the supernatant for microcarrier cell culture, the liquid at the lower position in the tank body 1 can be extracted through the pipette 6, and the problem that only a small part of liquid in a bioreactor can be extracted in the prior art is effectively solved.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims (10)

1. An apparatus for extracting supernatant for microcarrier cell culture, comprising: the stirring device comprises a stirring shaft, a filter, a stirring paddle and a pipette, wherein the stirring shaft is vertically arranged, and the filter, the stirring paddle and the pipette are arranged on the stirring shaft; the filter is cylindrical and has an opening at the upper end, the stirring shaft is inserted into the filter, and the filter is connected to the lower part of the stirring shaft; the outer side of the filter is provided with the stirring paddle; a pipette is inserted down into the filter interior at the upper end of the filter.
2. The device for extracting supernatant for microcarrier cell culture according to claim 1, wherein: the stirring rake includes flabellum and collar, the flabellum set up in the outside of collar, the collar is in the outside of filter is fixed through the screw.
3. The device for extracting supernatant for microcarrier cell culture according to claim 1, wherein: the stirring rake is for setting up in a plurality of on the filter.
4. The device for extracting supernatant for microcarrier cell culture according to claim 3, wherein: the stirring paddles are uniformly distributed in the axial direction of the filter.
5. The device for extracting supernatant for microcarrier cell culture according to any one of claims 1 to 4, wherein: the bottom of filter is provided with the mount pad, the lower extreme of (mixing) shaft pass through the screw install in on the mount pad.
6. The device for extracting supernatant for microcarrier cell culture according to any one of claims 1 to 4, wherein: the side wall of the filter is provided with filter holes, the device for extracting the supernatant from the microcarrier cell culture comprises a plurality of filters with different filter hole pore sizes, and the filters can be detachably arranged on the stirring shaft, so that the filters can be used in a replaceable way.
7. A bioreactor, characterized by: the device for extracting the supernatant from the microcarrier cell culture comprises a tank body, a top cover and the device for extracting the supernatant from the microcarrier cell culture according to any one of claims 1 to 6, wherein a bearing seat is arranged on the top cover, a stirring shaft is arranged on the bearing seat through a bearing, and the pipette penetrates out of the top cover and is connected to a peristaltic pump through a silica gel hose.
8. The bioreactor of claim 7, wherein: the pipette comprises a lower vertical section inserted into the filter, a bending section connected to the vertical section and an upper vertical section connected to the bending section; the lower vertical section is inserted into the inside bottom of the filter.
9. The bioreactor of claim 7, wherein: a bolt is arranged on the upper vertical section, an axial through hole is formed in the bolt, and the vertical section penetrates through the through hole; the bolt penetrates through the mounting hole in the top cover and then is screwed on a nut.
10. The bioreactor of any one of claims 7 to 9, wherein: the lower end of the filter is positioned at a position close to the bottom of the tank body.
CN202110501031.5A 2021-05-08 2021-05-08 Device for extracting supernatant in microcarrier cell culture and bioreactor Pending CN113122437A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110501031.5A CN113122437A (en) 2021-05-08 2021-05-08 Device for extracting supernatant in microcarrier cell culture and bioreactor

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Application Number Priority Date Filing Date Title
CN202110501031.5A CN113122437A (en) 2021-05-08 2021-05-08 Device for extracting supernatant in microcarrier cell culture and bioreactor

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113667595A (en) * 2021-08-11 2021-11-19 安徽霍尔斯工程技术有限公司 Moving bed bioreactor
CN116676192A (en) * 2023-06-07 2023-09-01 上海多宁生物科技股份有限公司 Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113667595A (en) * 2021-08-11 2021-11-19 安徽霍尔斯工程技术有限公司 Moving bed bioreactor
CN116676192A (en) * 2023-06-07 2023-09-01 上海多宁生物科技股份有限公司 Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof
CN116676192B (en) * 2023-06-07 2023-11-14 上海多宁生物科技股份有限公司 Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof

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