CN105060464B - A kind of biofilm sewage treatment device and biomembrane home position testing method - Google Patents
A kind of biofilm sewage treatment device and biomembrane home position testing method Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Biological Treatment Of Waste Water (AREA)
Abstract
The invention discloses a kind of biofilm sewage treatment device and biomembrane home position testing method, this device includes spill tank, biofilm carrier disc, rotating shaft, rotating shaft driving means, water inlet system and effluent collection system.The device that the present invention provides possesses sewage disposal and biomembrane two kinds of functions of sample collecting test in situ；Biomembrane strip on dismounting biofilm carrier disc carries out carrier disc surface biological film in-situ test, and that captures that traditional biological film sewage treatment device generally exists keeps biomembrane original structure form to carry out diffraction patterns for biomembrane samples in test process gathering a difficult problem for difficulty；The pulse power drives synchronous motor to drive biofilm carrier disc fixed-axis rotation, realize dead axle rotating speed real-time continuous to adjust, in order to maintain, biofilm structure remains complete, biofilm surface shearing force quantifies simulation provides necessary guarantee, solve conventional gearbox speed governing rotating speed and adjust difficulty in real time, saver Financial cost, reduces plant area area.
Description
Technical field
The present invention relates to a kind of biofilm sewage treatment device and biomembrane home position testing method, belong at biomembrane sewage
Reason and carrier surface biomembrane in-situ testing technique field.
Background technology
Biomembrane is by microorganism and surface secretions thereof, and the membranaceous material of other impurity composition.Biomembrane is for dirt
Water, converts the organic of biological utilisation in sewage mainly by apposition growth microbial metabolism in the biomembrane of the surface of solids
The materials such as thing, ammonia nitrogen, phosphorus, it is achieved sewage purification.Biomembrance process is a kind of common process of sewage disposal, has excess sludge and produces
Raw amount is few, and water quality adaptability is strong, is particularly well-suited to process sewage of low concentration, such as surface water, aquaculture aquaculture wastewater etc..By
It is the core of sewage disposal system in biomembrane, it is necessary to understand the relevant pass of Biofilm characteristics and wastewater treatment efficiency in depth
System, provides theoretical foundation for improving the treatment effect of biomembrane sewage disposal system.
It is employing granular solids in sewage disposal system currently, with respect to the general step processing the research of saprobia film
The fillers such as material, soft hairbrush filler or half soft composite plastic, form biomembrane for attached growth of microorganisms and provide attachment table
Face, after biofilm formation, changes sewage disposal system technological parameter, and collects diverse location under the conditions of different technical parameters
Filler, basis of microscopic observation biofilm thickness, and scrape filling surface biomembrane and carry out destructive sampling, carry out microbial species
Group multiformity, functional microorganism quantity test analysis, explore the dependency relation of biomembrane feature and wastewater treatment efficiency, for sewage
The technological design of biofilm treatment system and operational management provide theoretical foundation.But, this traditional research approach, it is impossible to anti-
Reflecting under filling surface biomembrane full-scale condition, inside and outside biomembrane inner texture structure, film, mass transport process, biomembrane are at filling surface
Adhesive force and biofilm detachment, microorganism and secretions spatial distribution characteristic thereof, microbe colony structure.It addition, by biology
Film filling surface area is little, and microscope observes that its surface biological film difficulty is high, accuracy is difficult to ensure.
Biomembrane inner texture structure directly affects dirty water pollutant in biomembrane internal mass transfer process, and unofficial biography in film
Matter efficiency affects existence and the growth of microorganism inside and outside film, thus affect its to the absorption of pollutant and degraded, this is in sewage
Pollutant removal has a direct impact effect.It addition, biomembrane exists close between the adhesive force and biofilm detachment of filling surface
The dependency relation cut.In the case of adhesive force is relatively strong, biomembrane difficult drop-off, can continue to go a good appetite suddenly appearing in a serious disease to play at wastewater pollutants
Bigger effect.Further, microorganism and secretions spatial distribution characteristic thereof by affect its to the adhesion of cell and cohesion, thus certainly
Determine the integrally-built stability of biomembrane.Therefore, it is necessary to maintaining the condition of biofilm structure, to biofilm structure and micro-life
Thing feature is deeply probed into.
Summary of the invention
The present invention solves a diffraction patterns for biomembrane samples in-situ study sampling difficult problem in saprobia film processing system, it is provided that Yi Zhongsheng
Thing film sewage treatment device and biomembrane home position testing method, biofilm microorganisms Extracellular polymers space under research in-situ condition
Distribution, biomembrane, at adhesion, biological denitrificaion functional microorganism abundance and the spatial distribution characteristic etc. of carrier surface, are given birth to for sewage
The design of thing film processing system and operational management provide theoretical foundation.
Biofilm sewage treatment device includes spill tank, water inlet system, effluent collection system and computer；
Wherein, spill tank is by horizontal semicircular cylinder and is individually fixed in the rectangular slab at semicircular cylinder two ends and forms, rectangular slab
Top edge is generally aligned in the same plane with the opening of semicircular cylinder, rectangular slab tall and big in the radius of semicircular cylinder, in the axis position of semicircular cylinder
Installed rotating shaft, the two ends of rotating shaft respectively by bearing and the connections of two pieces of rectangular slabs, one end of rotating shaft be fixed on this end moment shape
The output shaft of the synchronous motor on plate is connected, and synchronous motor is driven by the pulse power, drives rotating shaft to make its rotating speed at 1-300rpm
In the range of real-time continuous adjustable.Rotating shaft is coaxially fixed with the biofilm carrier disc of some circles, biofilm carrier
Respectively have 3-5 bar rectangular recess in the positive and negative card of disc, seamless in rectangular recess be embedded with and biomembrane
The biomembrane strip of the identical material of carrier disc, biomembrane strip outer surface and the card of biofilm carrier disc are in same flat
Face.Having inlet opening on one piece in two pieces of rectangular slabs of spill tank, another block has apopore, bottom has sewage emptying
Mouthful.Inlet opening and apopore are positioned at sustained height, and symmetry is divided into axially symmetric both sides of spill tank semicircular cylinder.
Water inlet system includes inlet pipeline, intake pump and for holding the collecting-tank for the treatment of sewage；Effluent collection system
Including going out water pump, outlet pipeline and the storage pool of sewage after holding process.The water inlet of intake pump is by inlet pipeline even
Connecing collecting-tank, the outlet of intake pump connects the inlet opening of spill tank；The apopore of spill tank and the water inlet going out water pump
Connecting, the outlet going out water pump connects storage pool by outlet pipeline.In collecting-tank with storage pool, it is equipped with ammonia nitrogen survey online
Electricity testing device and COD online testing device, and be connected with computer respectively.
In technique scheme, it is preferable that biofilm carrier disc thickness is 0.5～2cm, biofilm carrier disc radius
For spill tank radius 90%～95%.The a length of biofilm carrier disc radius of the rectangular recess on disc 85%～
100%, length-width ratio 10:1～10:2, the degree of depth is 1～3mm.
The method of biomembrane in-situ test comprises the steps:
1) open intake pump, sewage is pumped into spill tank, unbalanced pulse power drives synchronous motor by collecting-tank, drives
Rotating shaft, with 1～25rpm fixed-axis rotation, opens out water pump, and the sewage after processing is pumped into storage pool by spill tank；
2) the 35%～40% of liquid level submergence biofilm carrier disc area is made in spill tank；Collecting-tank and storage
Ammonia nitrogen online testing device in pond tests water inlet and water outlet ammonia nitrogen and COD concentration in the most every 24 hours with COD online testing device,
After often completing 24h measurement of concetration, drain the waste water in storage pool.When the water outlet ammonia nitrogens of continuous 3～5 days and COD concentration standard deviation
Time respectively in the range of ± 5%, device processes sewage effect and reaches stable, closes intake pump, goes out water pump and the pulse power, makes raw
Thing membrane carrier disc stops operating, and meets the biomembrane strip of testing requirement quantity under dismounting, carries out four groups of biomembranes and surveys in situ
Examination:
First group: carry out polysaccharide, protein fluorescence dyeing-Laser Scanning Confocal Microscope that biomembrane Extracellular polymers includes
Observation, it is thus achieved that microorganism Extracellular polymers spatial distribution image photo at the internal different-thickness of biomembrane；
Second group: be placed under atomic force microscope and rap biomembrane strip surface biomembrane acquisition biomembrane by probe
Mechanical characteristics data also analyze biofilm microorganisms adhesive force；
3rd group: by biomembrane strip surface biomembrane through fluorescence in situ hybridization-confocal microscopy, it is thus achieved that raw
At thing film different-thickness, ammonia oxidation bacteria, nitrite nitrogen oxidation bacteria and total antibacterial image photo, calculate ammonia oxidation bacteria, nitrite
Nitrogen oxidation bacteria accounts for the ratio of total antibacterial and carries out enrichment analysis；
4th group: be placed in microelectrode test system, it is thus achieved that ammonia nitrogen, dissolved oxygen, nitrate nitrogen at biomembrane internal mass transfer
Coefficient and mass transfer dynamics mathematical model；
3) by biofilm carrier disc reset, reopen intake pump, go out water pump and the pulse power, change amount of inlet water or
Change rotating shaft rotating speed, continue step 2)；
4) step 3 is repeated) until the test of the different inflows of experimental design and different rotating speeds Parameter Conditions all completes；
5) sewage in the result of each biomembrane in-situ test and this secondary pollutant film sewage treatment device is removed ammonia nitrogen and
COD concentration is compared, it is thus achieved that biofilm structure and microbial characteristic and the dependency relation of wastewater treatment efficiency.
The invention has the beneficial effects as follows:
(1) part biological membrane carrier disc is alternately immersed in sewage, and the biomembrane in carrier disc surface alternately exposes
In air and sewage, biofilm microorganisms is survived in aerobic and anaerobic environment and carries out metabolic activity, dirty water pollutant
Can by biofilm surface microorganism adsorption, degrade, convert decomposition, device has sewage treatment function, and device biomembrane carries simultaneously
Detachable biomembrane strip on body disc sheet, can be used for biomembrane sample collecting test in situ, and device possesses sewage disposal and life
Thing film two kinds of functions of sample collecting test in situ, the biomembrane overcoming traditional biological film sewage treatment device generally to exist divides in situ
Analysis difficulty, solves the problem that pure culture Biofilm test system does not possess sewage treatment function.
(2) device wastewater influent hole and apopore diagonally opposing corner are arranged, sewage-treatment plant uses spill tank and part leaching
The not mode of the biofilm carrier disc rotation in sewage, reduces saprobia membrane treatment appts water flow inside dead band, improves
Device sewage treating efficiency, promotes biomembrane homoepitaxial on biofilm carrier disc, and biofilm carrier disc surface is random
Rectangular recess is set, the embedded dismountable biomembrane strip of rectangular recess, biomembrane strip and carrier plate sheet material and roughness
Identical, biomembrane strip surface biomembrane is identical with biomembrane feature on biofilm carrier disc, by dismounting biomembrane strip,
To stick has biomembranous biomembrane strip to be placed on test instrunment to carry out the outer polymer dyeing-Laser Scanning Confocal Microscope of extracellular microbial
The test of image measurement, atomic force microscope biomembrane adhesive force, fluorescence in situ hybridization-Laser Scanning Confocal Microscope denitrification functions microorganism
Test, the internal ammonia nitrogen of microelectrode biomembrane, dissolved oxygen, the test of nitrate nitrogen mass transport process, capture traditional biological film sewage disposal
During the holding biomembrane original structure form that device generally exists is tested, diffraction patterns for biomembrane samples gathers a difficult difficult problem, by above-mentioned
Biomembrane in-situ test data result removes ammonia nitrogen with biofilm sewage treatment device sewage and COD effect is compared, and relatively passes
System method, for further clear and definite biomembrane in situ structure and microbial characteristic and the dependency relation of wastewater treatment efficiency, makes a living
The design of thing film sewage treatment device and operational management provide reliable microbiology theoretical foundation.
(3) pulse power that the present invention provides drives synchronous motor to drive biofilm carrier disc fixed-axis rotation, with tradition
Motor compared with the combined control system of gear-box, it is real that the combination of this pulse power and synchronous motor can realize fixed-axis rotation speed
Time continuous fine adjustment, in real time slightly change rotating shaft rotating speed is for maintaining biofilm structure to maintain complete, biofilm surface shearing force to quantify
Simulation provides necessary guarantee, it addition, with motor compared with gear-box conventional combination mode, use the pulse power and synchronous motor
The mode of compound mode drive shaft, it is not necessary in the form of gear speed governing of multiple different radiis, not only saver economy becomes
This, it is possible to plant area area is greatly decreased.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of biofilm sewage treatment device；
Fig. 2 is the side-looking structural representation of biofilm sewage treatment device middle concave tank；
Fig. 3 is the main TV structure schematic diagram of biofilm sewage treatment device middle concave tank；
Fig. 4 is Section A-A and section B-B figure in Fig. 3, a) is Section A-A, b) is section B-B；
Fig. 5 is the structural representation of biofilm carrier disc, a) is front view, b) be a) in C-C sectional view and
Enlarged drawing.
Wherein: 1-spill tank；2-biofilm carrier disc；3-rotating shaft；4-ammonia nitrogen online testing device；5-COD is online
Test device；6-inlet opening；7-apopore；8-sewage evacuation port；9-collecting-tank；10-inlet pipeline；11-intake pump；12-goes out
Water pump；13-outlet pipeline；14-bearing；15-rectangular recess；16-biomembrane strip；The 17-pulse power；18-synchronous motor；
19-computer；20-storage pool.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.
With reference to Fig. 1-5, the biofilm sewage treatment device of the present invention, including spill tank 1, water inlet system, effluent collection
System and computer 19；
Described spill tank 1 is by horizontal semicircular cylinder and is individually fixed in the rectangular slab at semicircular cylinder two ends and forms, rectangle
Plate top edge is generally aligned in the same plane with the opening of semicircular cylinder, rectangular slab tall and big in the radius of semicircular cylinder, at the axis of semicircular cylinder
Position is provided with rotating shaft 3, and the two ends of rotating shaft 3 are connected with two pieces of rectangular slabs by bearing 14 respectively, one end of rotating shaft 3 be fixed on this
The output shaft of the synchronous motor 18 on end rectangular slab is connected, and synchronous motor 18 is driven by the pulse power 17, coaxial line in rotating shaft 3
Be fixed with the biofilm carrier disc 2 of some circles, the positive and negative card of biofilm carrier disc 2 has the most randomly
3-5 bar rectangular recess 15, the seamless biomembrane strip 16 being embedded with material identical with biofilm carrier disc in rectangular recess 15,
Biomembrane strip 16 outer surface is in same plane with the card of biofilm carrier disc 2；
In two pieces of rectangular slabs of spill tank 1, have inlet opening 6 on one piece, another block has apopore 7, spill water
Sewage evacuation port 8 is had bottom groove 1；
Described water inlet system includes inlet pipeline 10, intake pump 11 and for holding the collecting-tank 9 for the treatment of sewage, institute
The effluent collection system stated includes water pump 12, outlet pipeline 13 and the storage pool 20 of sewage after holding process；Intake pump
The water inlet of 11 connects collecting-tank 9 by inlet pipeline 10, and the outlet of intake pump 11 connects the inlet opening 6 of spill tank 1, recessed
The apopore 7 of shape tank 1 is connected with the water inlet going out water pump 12, and the outlet going out water pump 12 connects storage by outlet pipeline 13
Pond 20, is equipped with ammonia nitrogen online testing device 4 and COD online testing device 5 in collecting-tank 9 with storage pool 20, and ammonia nitrogen is online
Test device 4 and COD online testing device 5 is connected with computer 19 respectively.
Embodiment 1:
The hydraulic detention time Study on Microcosmic Mechanism to biofilm sewage treatment device treatment effect
Using 3 set biofilm sewage treatment devices to carry out parallel testing, often set biofilm sewage treatment device uses 6 lifes
Thing membrane carrier disc, disc is diameter 25cm, the disk of thickness 0.6cm, and dish spacing is 3cm, and every disc is randomly provided 8
When the Altitude control spill tank of biomembrane strip, inlet opening and apopore has Inlet and outlet water, disc immersion rate in sewage is
40%.
Circular biofilm carrier disc is to use activated sludge inocalation method that it is carried out biofilm, and method is as follows: by city
Sewage treatment plant residual mud does seed sludge and mixes according to a certain percentage with the artificial preparation simulation sewage containing 40mg/L ammonia nitrogen
It is stored in collecting-tank, pumps in spill tank by intake pump, flow rate pump 1.6L/h, utilize the pulse power to drive simultaneously and synchronize
Motor, drives biofilm carrier disc fixing in rotating shaft and rotating shaft with the speed fixed-axis rotation of 4rpm, utilizes ammonia nitrogen to survey online
Test system and every 24 hours of COD concentration Online Transaction Processing are to sewage after holding the collecting-tank for the treatment of sewage and depositing process
Storage pool in water body ammonia nitrogen concentration and COD concentration test, test terminates emptying and deposits the storage of sewage after process every time
Water body, data reach computer recording analysis, observe the biofilm situation on disc simultaneously, until disc surfaces appearance one layer is light
In yellow film, and water outlet, ammonia nitrogen concentration and the change in every 24 hours of COD concentration are in the range of ± 5%, and biofilm carrier disc is hung
Film success, carrying out hydraulic detention time affects test to biofilm sewage treatment device treatment effect and biomembrane feature.
Use same artificial preparation simulation sewage, keep pulse power output constant so that rotating shaft drives biological membranous disc
Sheet is respectively 2.4L/h (corresponding hydraulic detention time is 2h) with 4rpm uniform rotation, the water intake velocity changing intake pump, utilizes
Online Transaction Processing, test water inlet and water outlet ammonia nitrogen and the concentration of COD, after completing COD and ammonia nitrogen test, empty storeroom every time
The storage pool of sewage after reason.When in water, ammonia nitrogen concentration and COD concentration diurnal variation are in the range of ± 5%, system is considered as stable, meter
Calculate the clearance (formula 1) of ammonia nitrogen and COD.
R (%)=(Cin-Cout)/Cin× 100% (1)
Wherein, CinFor influent COD or ammonia nitrogen concentration (mg/L), CoutFor water outlet COD or ammonia nitrogen concentration (mg/L), R is for removing
Rate (%).
Device intake pump stops water inlet, and biofilm carrier disc stops operating, dismounting biofilm carrier disc and surface thereof
Rectangular recess in fixing 4 biomembrane strips, carry out polysaccharide, protein fluorescence that biomembrane Extracellular polymers includes respectively
The observation of dyeing-Laser Scanning Confocal Microscope, the test of biofilm microorganisms adhesive force atomic force microscope, biomembrane ammonia oxidation bacteria with
Nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-confocal microscopy, the internal ammonia nitrogen of biomembrane, dissolved oxygen biography
Matter process microelectrode is tested.
After biomembrane strip has been dismantled, immediately biofilm carrier disc is reinstalled origin-location, reopen intake pump,
Going out water pump and the pulse power, changing water inlet flow velocity is 1.2L/h (corresponding hydraulic detention time is 4h), utilizes Online Transaction Processing,
Test water inlet and water outlet ammonia nitrogen and the concentration of COD, when in water, ammonia nitrogen concentration and COD concentration diurnal variation are in the range of ± 5%, be
System is considered as stable, calculates the clearance (formula 1) of ammonia nitrogen and COD.Device intake pump stops water inlet, and biofilm carrier disc stops turning
4 dynamic, fixing in the rectangular recess on dismounting biofilm carrier disc and surface thereof biomembrane strips, carry out biomembrane born of the same parents respectively
Polysaccharide, protein fluorescence dyeing-Laser Scanning Confocal Microscope that outer polymer includes are observed, biofilm microorganisms adhesive force atom
Force microscope is tested, biomembrane ammonia oxidation bacteria is micro-with nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-copolymerization Jiao
The internal ammonia nitrogen of sem observation, biomembrane, the test of dissolved oxygen mass transport process microelectrode.
After biomembrane strip has been dismantled, immediately biofilm carrier disc is reinstalled origin-location, reopen intake pump,
Going out water pump and the pulse power, changing water inlet flow velocity is 0.9L/h (corresponding hydraulic detention time is 5h), utilizes Online Transaction Processing,
Test water inlet and water outlet ammonia nitrogen and the concentration of COD, when in water, ammonia nitrogen concentration and COD concentration diurnal variation are in the range of ± 5%, be
System is considered as stable, calculates the clearance (formula 1) of ammonia nitrogen and COD.Device intake pump stops water inlet, and biofilm carrier disc stops turning
Dynamic, dismantle 4 biomembrane strips, carry out biomembrane in-situ test, by difference flow of inlet water (corresponding different hydraulic detention times)
The observation of fluorescent dyeing-Laser Scanning Confocal Microscope obtain microorganism Extracellular polymers space at the internal different-thickness of biomembrane and divide
Cloth image photo, atomic force microscope obtains mechanics of biomembranes characteristic, the ammonia oxidation bacteria of image software acquisition, nitrite
Nitrogen oxidation bacteria abundance, microelectrode test system obtain ammonia nitrogen, dissolved oxygen, nitrate nitrogen in biomembrane internal mass transfer coefficient and biography
Matter dynamics mathematical model, carries out being compared confirmation, and is stopped from different waterpower by above-mentioned biomembrane in-situ test data result
Removal ammonia nitrogen and the COD effect of staying the time are compared, and specify biofilm structure and microbial characteristic and different hydraulic retentions
The dependency relation of the wastewater treatment efficiency represented with pollutants removal rate under time conditions, discloses different hydraulic detention time to life
The microcosmic mechanism of thing film sewage treatment device wastewater treatment efficiency impact, and obtain optimal water power standing time.
Embodiment 2:
The flow shear Study on Microcosmic Mechanism to biofilm sewage treatment device treatment effect
Reporting according to current document, the flow shear that biomembrane can tolerate is in the range of 0～2Pa.Use example 1 institute
The device stated and sewage, keep biofilm sewage treatment device flow rate pump constant, at the optimal water power standing time that example 1 determines
Under the conditions of, by changing the output of the pulse power, change the rotating speed of rotating shaft, it is thus achieved that different biofilm surface flow shear bars
Part.Wherein under the conditions of different rotating speeds at biofilm carrier disc different radiuses of rotation current to biomembranous shearing force according to calculating
Formula (2) calculates.
Wherein ρ is fluid density (kg/m3), r is the radius (m) at sampling, and υ is liquid motion viscosity (m2/ s), ω is angle
Speed (r/s), τ is shearing force (Pa).Under the conditions of different flow shears, test biofilm sewage treatment device sewage disposal
Effect, and keep biofilm structure that Biofilm characteristics is carried out in-situ test, specifically comprise the following steps that
Under the conditions of rotating speed is 4rpm, when in water outlet, ammonia nitrogen concentration and COD concentration diurnal variation are in the range of ± 5%, raw
Thing film system reaches stable, pulls down 4 biomembrane strips, carries out polysaccharide, protein that biomembrane Extracellular polymers includes respectively
Fluorescent dyeing-Laser Scanning Confocal Microscope observation, the test of biofilm microorganisms adhesive force atomic force microscope, biomembrane ammoxidation
Bacterium and nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-confocal microscopy, biomembrane internal ammonia nitrogen, dissolving
Oxygen transfer process microelectrode is tested.
After biomembrane strip has been dismantled, immediately biofilm carrier disc is reinstalled origin-location, reopen intake pump,
Going out water pump and the pulse power, change the output of the pulse power, making to change biofilm carrier disc rotation speed is 8rpm, and in water outlet, ammonia nitrogen is dense
When degree and COD concentration diurnal variation are in the range of ± 5%, biomembrane system reaches stable, calculates pollutant removal according to formula (1)
Rate, then pull down 4 biomembrane strips, carry out biomembrane in-situ test, repeat the above steps, change the defeated of the pulse power successively
Going out, making to change biofilm carrier disc rotation speed is 12rpm, 20rpm, 28rpm, 40rpm, 52rpm, 60rpm, measures steady statue
Biomembrane system wastewater pollutants ammonia nitrogen, COD clearance, and it is glimmering to complete polysaccharide that biomembrane Extracellular polymers includes, protein
The observation of photoinitiator dye dyeing-Laser Scanning Confocal Microscope, the test of biofilm microorganisms adhesive force atomic force microscope, biomembrane ammonia oxidation bacteria
With nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-confocal microscopy, biomembrane internal ammonia nitrogen, dissolved oxygen
Mass transport process microelectrode is tested, and specifies the flow shear influential effect to biofilm sewage treatment device wastewater treatment efficiency,
Flow shear is disclosed to biofilm sewage treatment device wastewater treatment efficiency influence in conjunction with biomembrane in-situ test result
Microcosmic mechanism.
Claims (6)
1. the method that application biofilm sewage treatment device carries out biomembrane in-situ test, described biofilm sewage treatment device
Including spill tank (1), water inlet system, effluent collection system and computer (19)；
Described spill tank (1) is by horizontal semicircular cylinder and is individually fixed in the rectangular slab at semicircular cylinder two ends and forms, rectangular slab
Top edge is generally aligned in the same plane with the opening of semicircular cylinder, rectangular slab tall and big in the radius of semicircular cylinder, in the axis position of semicircular cylinder
Having installed rotating shaft (3), the two ends of rotating shaft (3) are connected with two pieces of rectangular slabs by bearing (14) respectively, and one end of rotating shaft (3) is with solid
The output shaft of the synchronous motor (18) on this end rectangular slab is connected, and synchronous motor (18) is driven by the pulse power (17),
The biofilm carrier disc (2) of some circles, the positive helical cast of biofilm carrier disc (2) coaxially it is fixed with in rotating shaft (3)
Have 3-5 bar rectangular recess (15) on face the most randomly, seamless in rectangular recess (15) be embedded with and biofilm carrier dish
The biomembrane strip (16) of the identical material of sheet, biomembrane strip (16) outer surface is in the card of biofilm carrier disc (2)
Same plane；
In two pieces of rectangular slabs of spill tank (1), on one piece, have inlet opening (6), another block has apopore (7), spill
Tank (1) bottom has sewage evacuation port (8)；
Described water inlet system includes inlet pipeline (10), intake pump (11) and is used for holding the collecting-tank (9) for the treatment of sewage,
Described effluent collection system includes water pump (12), outlet pipeline (13) and the storage pool of sewage after holding process
(20)；The water inlet of intake pump (11) connects collecting-tank (9) by inlet pipeline (10), and the outlet of intake pump (11) connects recessed
The inlet opening (6) of shape tank (1), the apopore (7) of spill tank (1) is connected with the water inlet going out water pump (12), goes out water pump
(12) outlet connects storage pool (20) by outlet pipeline (13), is equipped with ammonia in collecting-tank (9) with storage pool (20)
Nitrogen online testing device (4) and COD online testing device (5), ammonia nitrogen online testing device (4) and COD online testing device (5)
It is connected with computer (19) respectively；
It is characterized in that, described method comprises the steps:
1) opening intake pump (11), by collecting-tank (9), sewage is continuously pumped into spill tank (1), unbalanced pulse power supply (17) drives
Dynamic synchronous motor, drives rotating shaft (3) with 1 ~ 25 rpm fixed-axis rotation, opens out water pump (12), and the sewage after processing is by spill
Tank (1) pumps into storage pool (20)；
2) the 35% ~ 40% of spill tank (1) interior liquid level submergence biofilm carrier disc area is made；Collecting-tank (9) and storage
The test water inlet in the most every 24 hours of ammonia nitrogen online testing device (4) in pond (20) and COD online testing device (5) and water outlet ammonia nitrogen
And COD concentration, after testing, drain the waste water in storage pool every time；When the water outlet ammonia nitrogens of continuous 3 ~ 5 days and COD concentration standard
When difference is not in the range of ± 5%, device processes sewage effect and reaches stable, closes intake pump (11), goes out water pump (12) and pulse
Power supply (17), makes biofilm carrier disc (2) stop operating, and meets the biomembrane strip (16) of testing requirement quantity under dismounting,
Carry out four groups of biomembrane in-situ tests:
First group: carry out polysaccharide, protein fluorescence dyeing-Laser Scanning Confocal Microscope sight that biomembrane Extracellular polymers includes
Survey, it is thus achieved that microorganism Extracellular polymers spatial distribution image photo at the internal different-thickness of biomembrane；
Second group: be placed under atomic force microscope and rap biomembrane strip surface biomembrane acquisition mechanics of biomembranes by probe
Characteristic also analyzes biofilm microorganisms adhesive force；
3rd group: by biomembrane strip surface biomembrane through fluorescence in situ hybridization-confocal microscopy, it is thus achieved that biomembrane
At different-thickness, ammonia oxidation bacteria, nitrite nitrogen oxidation bacteria and total antibacterial image photo, calculate ammonia oxidation bacteria, nitrite nitrogen oxygen
Change bacterium accounts for the ratio of total antibacterial and carries out enrichment analysis；
4th group: be placed in microelectrode test system, it is thus achieved that ammonia nitrogen, dissolved oxygen, nitrate nitrogen at biomembrane internal mass transfer coefficient
And mass transfer dynamics mathematical model；
3) by biofilm carrier disc reset, reopen intake pump (11), go out water pump (12) and the pulse power (17), change into
The water water yield or change rotating shaft rotating speed, continue step 2)；
4) step 3) is repeated until the test of the different inflows of experimental design and different rotating speeds Parameter Conditions all completes；
5) result of each biomembrane in-situ test is removed ammonia nitrogen with sewage in this secondary pollutant film sewage treatment device and COD is dense
Degree is compared, it is thus achieved that biofilm structure and microbial characteristic and the dependency relation of wastewater treatment efficiency.
The method that application biofilm sewage treatment device the most according to claim 1 carries out biomembrane in-situ test, it is special
Levying and be, described biofilm carrier disc (2) thickness is 0.5 ~ 2 cm, and described rectangular recess (15) degree of depth is 1 ~ 3mm.
The method that application biofilm sewage treatment device the most according to claim 1 carries out biomembrane in-situ test, it is special
Levy and be, the 85% ~ 100% of a length of biofilm carrier disc (2) radius of described rectangular recess (15), length-width ratio 10:1 ~
10:2.
The method that application biofilm sewage treatment device the most according to claim 1 carries out biomembrane in-situ test, it is special
Levying and be, described biofilm carrier disc (2) radius is spill tank radius 90% ~ 95%.
The method that application biofilm sewage treatment device the most according to claim 1 carries out biomembrane in-situ test, it is special
Levying and be, described inlet opening (6) and apopore (7) are positioned at sustained height, and symmetry is divided into spill tank (1) semicircular cylinder
Axially symmetric both sides.
The method that application biofilm sewage treatment device the most according to claim 1 carries out biomembrane in-situ test, it is special
Levying and be, rotating speed real-time continuous in the range of 1-300rpm of described rotating shaft (3) is adjustable.
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