CN105060464A - Biofilm sewage treatment device and biofilm in-situ testing method - Google Patents
Biofilm sewage treatment device and biofilm in-situ testing method Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
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Abstract
The invention discloses a biofilm sewage treatment device and a biofilm in-situ testing method. The device comprises a concave water tank, a biofilm carrier disk, a rotating shaft, a rotating shaft driving device, a water inlet system and an effluent collecting system. The device disclosed by the invention has two functions of sewage treatment and biofilm in-situ sample collecting and testing; the biofilm in-situ test on the carrier disk is carried out through dismounting a biofilm test strip on the biofilm carrier disk, so that the ubiquitous problem that a biofilm sample is difficult to collect in the process of testing by keeping the original structural form of a biofilm in the traditional biofilm sewage treatment device is solved; a synchronous motor is driven by a pulsed power supply to drive the biofilm carrier disk to rotate around a fixed shaft, so that the real-time continuous regulation of the rotating speed of the fixed shaft is realized, the necessary guarantee is provided for maintaining the integrity of the structure of the biofilm and quantitative simulation of a shearing force on the surface of the biofilm, the difficulty for regulating the rotating speed of a traditional gearbox in real time is solved, the economic cost of the device is reduced, and the area occupied by the device is reduced.
Description
Technical field
The present invention relates to a kind of biofilm sewage treatment device and microbial film home position testing method, belong to microbial film sewage disposal and carrier surface microbial film in-situ testing technique field.
Background technology
Microbial film is by microorganism and surperficial secretory product thereof, and the membranaceous material of other impurity composition.Microbial film is used for sewage, mainly utilizes apposition growth microbial metabolism in the microbial film of solid surface to transform the material such as organism, ammonia nitrogen, phosphorus of biological utilisation in sewage, realizes sewage purification.Biomembrance process is a kind of common process of sewage disposal, has excess sludge generation few, and water quality adaptability is strong, is specially adapted to process sewage of low concentration, as surface water, aquaculture aquaculture wastewater etc.Due to the core that microbial film is Sewage treatment systems, be necessary the correlationship of understanding Biofilm characteristics and wastewater treatment efficiency in depth, for the treatment effect improving microbial film Sewage treatment systems provides theoretical foundation.
At present, general step about microbial film research of disposing of sewage is in Sewage treatment systems, adopt granular solids material, the fillers such as soft hairbrush filler or half soft composite plastic, attaching surface is provided for attached growth of microorganisms forms microbial film, after biofilm formation, change Sewage treatment systems processing parameter, and under different technical parameters condition, collect the filler of different positions, basis of microscopic observation biofilm thickness, and scraping filling surface microbial film carries out destructiveness sampling, carry out microbial population diversity, functional microorganism quantity test analysis, explore the correlationship of microbial film feature and wastewater treatment efficiency, for the technological design of saprobia film processing system and operational management provide theoretical foundation.But, this traditional research approach, under can not reflecting filling surface microbial film full-scale condition, mass transfer process inside and outside microbial film inner texture structure, film, microbial film are in the bonding strength of filling surface and biofilm detachment, microorganism and secretory product spatial distribution characteristic, microbe colony structure.In addition, by little for biofilm packing surface area, its surface biological film difficulty of microscopic examination is high, accuracy is difficult to ensure.
Microbial film inner texture structure directly affects dirty water pollutant in microbial film internal mass transfer process, and mass-transfer efficiency affects existence and the growth of microorganism inside and outside film inside and outside film, thus affecting it to the absorption of pollutent and degraded, this has a direct impact effect to pollutant removal in sewage.In addition, there is close correlationship in microbial film between the bonding strength and biofilm detachment of filling surface.When bonding strength is stronger, microbial film difficult drop-off, can continue to play larger effect in wastewater pollutants is removed.Further, microorganism and secretory product spatial distribution characteristic thereof will affect it to the adhesion of cell and cohesion, thus determine the integrally-built stability of microbial film.Therefore, be necessary, in the condition maintaining biofilm structure, deeply to probe into biofilm structure and microorganism feature.
Summary of the invention
The present invention is for solving a diffraction patterns for biomembrane samples in-situ study sampling difficult problem in saprobia film processing system, a kind of biofilm sewage treatment device and microbial film home position testing method are provided, under research in-situ condition, biofilm microorganisms Extracellular polymers spatial distribution, microbial film are at the adhesive power of carrier surface, biological denitrificaion functional microorganism abundance and spatial distribution characteristic etc., for the management of saprobia film processing system design and operation provides theoretical foundation.
Biofilm sewage treatment device comprises spill tank, water inlet system, effluent collection system and computer;
Wherein, spill tank is made up of horizontal semicircular cylinder and the rectangular plate that is individually fixed in semicircular cylinder two ends, the opening of rectangular plate upper limb and semicircular cylinder is positioned at same plane, rectangular plate tall and big in the radius of semicircular cylinder, rotating shaft is provided with in the axial location of semicircular cylinder, the two ends of rotating shaft are connected with two pieces of rectangular plates respectively by bearing, one end of rotating shaft is connected with the output shaft of the synchronous machine be fixed on this end rectangular plate, synchronous machine is driven by the pulse power, drives rotating shaft to make its rotating speed real-time continuous within the scope of 1-300rpm adjustable.Rotating shaft is coaxially fixed with the biofilm carrier disc of some circles, the positive and negative card of biofilm carrier disc radially respectively has 3-5 bar rectangular recess randomly, in rectangular recess, the seamless microbial film being embedded with material identical with biofilm carrier disc tries bar, and the card of microbial film examination bar outside surface and biofilm carrier disc is in same plane.In two pieces of rectangular plates of spill tank, one piece has prosopyle, another block has posticum, and bottom has sewage evacuation port.Prosopyle and posticum are positioned at sustained height, and symmetry is divided into the axial plane of symmetry both sides of spill tank semicircular cylinder.
Water inlet system comprises suction culvert, intake pump and the water collecting basin for holding treatment sewage; Effluent collection system comprises water pump, outlet pipeline and the storage pool for holding the rear sewage of process.The water-in of intake pump connects water collecting basin by suction culvert, and the water outlet of intake pump connects the prosopyle of spill tank; The posticum of spill tank is connected with the water-in going out water pump, and the water outlet going out water pump connects storage pool by outlet pipeline.In water collecting basin and storage pool, be equipped with ammonia nitrogen online testing device and COD online testing device, and be connected with computer respectively.
In technique scheme, preferably, biofilm carrier disc thickness is 0.5 ~ 2cm, and biofilm carrier disc radius is spill tank radius 90% ~ 95%.The length of the rectangular recess on disc is 85% ~ 100%, long-width ratio 10:1 ~ 10:2 of biofilm carrier disc radius, and the degree of depth is 1 ~ 3mm.
The method of microbial film in situ test comprises the steps:
1) open intake pump, sewage is pumped into spill tank by water collecting basin, unbalanced pulse power drives synchronous machine, drive rotating shaft with 1 ~ 25rpm fixed-axis rotation, open out water pump, the sewage after process is pumped into storage pool by spill tank;
2) 35% ~ 40% of liquid level submergence biofilm carrier disc area in spill tank is made; Ammonia nitrogen online testing device in water collecting basin and storage pool and COD online testing device are tested and are intake and water outlet ammonia nitrogen and COD concentration for all every 24 hours, after often completing 24h measurement of concetration, drain the waste water in storage pool.When the water outlet ammonia nitrogens of continuous 3 ~ 5 days and COD concentration standard difference be not in ± 5% scope, device effect of disposing of sewage reaches stable, close intake pump, go out water pump and the pulse power, biofilm carrier disc is stopped operating, meet the microbial film examination bar of testing requirement quantity under dismounting, carry out four groups of microbial film in situ tests:
First group: carry out polysaccharide that microbial film Extracellular polymers includes, protein fluorescence dyeing-Laser Scanning Confocal Microscope observation, obtain microbial film inner different thickness place microorganism Extracellular polymers spatial distribution image photo;
Second group: rap microbial film strip surface microbial film by probe under being placed in atomic force microscope and obtain mechanics of biomembranes characteristic and analyze biofilm microorganisms bonding strength;
3rd group: by microbial film strip surface microbial film through fluorescence in situ hybridization-confocal microscopy, obtain microbial film different thickness place ammonia oxidation bacteria, nitrite nitrogen oxidation bacteria and total bacterium image photo, the ratio that calculating ammonia oxidation bacteria, nitrite nitrogen oxidation bacteria account for total bacterium carries out enrichment analysis;
4th group: be placed in microelectrode test macro, obtain ammonia nitrogen, dissolved oxygen, nitrate nitrogen in microbial film internal mass transfer coefficient and mass transfer dynamics mathematical model;
3) biofilm carrier disc is resetted, reopen intake pump, go out water pump and the pulse power, change amount of inlet water or change rotating shaft rotating speed, continuing step 2);
4) repeating step 3) until the different flooding quantity of experimental design and the test of different rotating speeds Parameter Conditions all complete;
5) sewage in the result of each microbial film in situ test and this secondary pollutant film sewage treatment device is removed ammonia nitrogen and COD concentration is compared, obtain the correlationship of biofilm structure and microbial characteristic and wastewater treatment efficiency.
The invention has the beneficial effects as follows:
(1) part biological membrane carrier disc alternately immerse is in sewage, microbial film on carrier disc surface is alternately exposed in air and sewage, biofilm microorganisms is survived and is carried out Metabolic activity in aerobic and anaerobic environment, dirty water pollutant can by biofilm surface microorganism adsorption, degraded, transform and decompose, device has sewage treatment function, with the detachable microbial film examination bar on timer biofilm carrier disc, can be used for the test of microbial film original position sample collecting, device possesses sewage disposal and microbial film original position sample collecting tests two kinds of functions, overcome the ubiquitous microbial film in-situ study difficulty of traditional biological film sewage treatment device, solve the problem that pure culture Biofilm test system does not possess sewage treatment function.
(2) device wastewater influent hole and posticum diagonally opposing corner are arranged, waste disposal plant adopts spill tank and is partially immersed in the mode of the biofilm carrier disc rotation in sewage, reduce saprobia membrane treatment appts water flow inside dead band, improve device sewage treating efficiency, promote microbial film homoepitaxial on biofilm carrier disc, biofilm carrier disc surface arranges rectangular recess at random, the embedded dismountable microbial film examination bar of rectangular recess, microbial film examination bar is identical with carrier plate sheet material and roughness, microbial film strip surface microbial film is identical with microbial film feature on biofilm carrier disc, by dismounting microbial film examination bar, to stick has biomembranous microbial film to try bar to be placed in and testing tool to carry out the outer polymer dyeing-confocal images of extracellular microbial and test, atomic force microscope microbial film bonding strength is tested, fluorescence in situ hybridization-Laser Scanning Confocal Microscope denitrification functions microorganism testing, the inner ammonia nitrogen of microelectrode microbial film, dissolved oxygen, nitrate nitrogen mass transfer process is tested, capture traditional biological film sewage treatment device ubiquitous maintenance microbial film original structure form test in diffraction patterns for biomembrane samples gather difficulty a difficult problem, above-mentioned microbial film in situ test data results and biofilm sewage treatment device sewage are removed ammonia nitrogen and COD effect is compared, comparatively traditional method, for the correlationship of further clear and definite microbial film original position structure and microbial characteristic and wastewater treatment efficiency, for the management of biofilm sewage treatment device design and operation provides reliable microbiology theoretical foundation.
(3) pulse power provided by the invention drives synchronous machine to drive biofilm carrier disc fixed-axis rotation, with traditional motor compared with the combination control system of wheel casing, this pulse power and synchronous machine combine and can realize fixed-axis rotation speed real-time continuous and finely tune, slightly change rotating shaft rotating speed remains complete for maintaining biofilm structure in real time, biofilm surface shearing force quantitative simulation provides necessary guarantee, in addition, with motor compared with wheel casing conventional combination mode, adopt the mode of the array mode drive shaft of the pulse power and synchronous machine, without the need in the form of gear speed governing of multiple different radii, not only economizer Financial cost, also plant area area can significantly be reduced.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of biofilm sewage treatment device;
Fig. 2 is the side-looking structural representation of biofilm sewage treatment device middle concave tank;
Fig. 3 is the main TV structure schematic diagram of biofilm sewage treatment device middle concave tank;
Fig. 4 is A-A cross section and B-B sectional view in Fig. 3, and a) being wherein A-A cross section, b) is B-B cross section;
Fig. 5 is the structural representation of biofilm carrier disc, a) is wherein front view, b) is C-C sectional view and enlarged view thereof in a).
Wherein: 1-spill tank; 2-biofilm carrier disc; 3-rotating shaft; 4-ammonia nitrogen online testing device; 5-COD online testing device; 6-prosopyle; 7-posticum; 8-sewage evacuation port; 9-water collecting basin; 10-suction culvert; 11-intake pump; 12-goes out water pump; 13-outlet pipeline; 14-bearing; 15-rectangular recess; 16-microbial film examination bar; The 17-pulse power; 18-synchronous machine; 19-computer; 20-storage pool.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
With reference to Fig. 1-5, biofilm sewage treatment device of the present invention, comprises spill tank 1, water inlet system, effluent collection system and computer 19;
Described spill tank 1 is made up of horizontal semicircular cylinder and the rectangular plate that is individually fixed in semicircular cylinder two ends, the opening of rectangular plate upper limb and semicircular cylinder is positioned at same plane, rectangular plate tall and big in the radius of semicircular cylinder, rotating shaft 3 is provided with in the axial location of semicircular cylinder, the two ends of rotating shaft 3 are connected with two pieces of rectangular plates respectively by bearing 14, one end of rotating shaft 3 is connected with the output shaft of the synchronous machine 18 be fixed on this end rectangular plate, synchronous machine 18 is driven by the pulse power 17, rotating shaft 3 is coaxially fixed with the biofilm carrier disc 2 of some circles, the positive and negative card of biofilm carrier disc 2 radially has 3-5 bar rectangular recess 15 randomly, in rectangular recess 15, the seamless microbial film being embedded with material identical with biofilm carrier disc tries bar 16, the card of microbial film examination bar 16 outside surface and biofilm carrier disc 2 is in same plane,
In two pieces of rectangular plates of spill tank 1, one piece has prosopyle 6, another block has posticum 7, bottom spill tank 1, have sewage evacuation port 8;
Described water inlet system comprises suction culvert 10, intake pump 11 and the water collecting basin 9 for holding treatment sewage, and described effluent collection system comprises water pump 12, outlet pipeline 13 and the storage pool 20 for holding the rear sewage of process; The water-in of intake pump 11 connects water collecting basin 9 by suction culvert 10, the water outlet of intake pump 11 connects the prosopyle 6 of spill tank 1, the posticum 7 of spill tank 1 is connected with the water-in going out water pump 12, the water outlet going out water pump 12 connects storage pool 20 by outlet pipeline 13, in water collecting basin 9 with storage pool 20, be equipped with ammonia nitrogen online testing device 4 and COD online testing device 5, ammonia nitrogen online testing device 4 is connected with computer 19 respectively with COD online testing device 5.
Embodiment 1:
Hydraulic detention time is to the Study on Microcosmic Mechanism of biofilm sewage treatment device treatment effect
3 cover biofilm sewage treatment devices are adopted to carry out parallel testing, often overlap biofilm sewage treatment device and adopt 6 biofilm carrier discs, disc is the disk of diameter 25cm, thickness 0.6cm, dish spacing is 3cm, every disc is arranged at random 8 microbial film examination bars, when the Altitude control spill tank of prosopyle and posticum has an Inlet and outlet water, disc immersion rate in sewage is 40%.
Circular biofilm carrier disc adopts active sludge inoculation method to carry out biofilm to it, method is as follows: excess sludge of municipal sewage plant is done seed sludge and manually prepare the simulated sewage containing 40mg/L ammonia nitrogen and mix according to a certain percentage and be stored in water collecting basin, pump in spill tank by intake pump, flow rate pump 1.6L/h, utilize the pulse power to drive synchronous machine simultaneously, drive biofilm carrier disc fixing in rotating shaft and rotating shaft with the speed fixed-axis rotation of 4rpm, utilize ammonia nitrogen Online Transaction Processing and every 24 hours of COD concentration Online Transaction Processing to hold treatment sewage water collecting basin and after depositing process sewage storage pool in water body ammonia nitrogen concentration and COD concentration test, each test terminates the emptying storage pool water body depositing the rear sewage of process, data reach computer recording analysis, observe the biofilm situation on disc simultaneously, until there is the faint yellow film of one deck in disc surfaces, and ammonia nitrogen concentration and COD concentration change in ± 5% scope for every 24 hours in water outlet, the success of biofilm carrier disc biofilm, carry out hydraulic detention time to biofilm sewage treatment device treatment effect and microbial film feature impact test.
Adopt same artificial preparation simulated sewage, the pulse power is kept to export constant, rotating shaft is made to drive microbial film disc with 4rpm uniform rotation, the water intake velocity changing intake pump is respectively 2.4L/h (corresponding hydraulic detention time is 2h), utilize Online Transaction Processing, test the concentration of water inlet and water outlet ammonia nitrogen and COD, after completing COD and ammonia nitrogen test at every turn, the emptying storage pool depositing the rear sewage of process.When ammonia nitrogen concentration in water and the daily variation of COD concentration are in ± 5% scope, system is considered as stablizing, and calculates the clearance (formula 1) of ammonia nitrogen and COD.
R(％)＝(C
in-C
out)/C
in×100％(1)
Wherein, C
infor influent COD or ammonia nitrogen concentration (mg/L), C
outfor water outlet COD or ammonia nitrogen concentration (mg/L), R is clearance (%).
Device intake pump stops water inlet, biofilm carrier disc stops operating, 4 microbial films examination bars of the rectangular recess internal fixtion on dismounting biofilm carrier disc and surface thereof, carry out polysaccharide that microbial film Extracellular polymers includes respectively, protein fluorescence dyeing-Laser Scanning Confocal Microscope observation, the test of biofilm microorganisms bonding strength atomic force microscope, microbial film ammonia oxidation bacteria and nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-confocal microscopy, microbial film inside ammonia nitrogen, dissolved oxygen mass transfer process microelectrode test.
After microbial film examination bar has been dismantled, immediately biofilm carrier disc is reinstalled home position, reopen intake pump, go out water pump and the pulse power, changing water inlet flow velocity is 1.2L/h (corresponding hydraulic detention time is 4h), utilize Online Transaction Processing, the concentration of test water inlet and water outlet ammonia nitrogen and COD, when ammonia nitrogen concentration in water and the daily variation of COD concentration are in ± 5% scope, system is considered as stablizing, and calculates the clearance (formula 1) of ammonia nitrogen and COD.Device intake pump stops water inlet, biofilm carrier disc stops operating, 4 microbial films examination bars of the rectangular recess internal fixtion on dismounting biofilm carrier disc and surface thereof, carry out polysaccharide that microbial film Extracellular polymers includes respectively, protein fluorescence dyeing-Laser Scanning Confocal Microscope observation, the test of biofilm microorganisms bonding strength atomic force microscope, microbial film ammonia oxidation bacteria and nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-confocal microscopy, microbial film inside ammonia nitrogen, dissolved oxygen mass transfer process microelectrode test.
After microbial film examination bar has been dismantled, immediately biofilm carrier disc is reinstalled home position, reopen intake pump, go out water pump and the pulse power, changing water inlet flow velocity is 0.9L/h (corresponding hydraulic detention time is 5h), utilize Online Transaction Processing, the concentration of test water inlet and water outlet ammonia nitrogen and COD, when ammonia nitrogen concentration in water and the daily variation of COD concentration are in ± 5% scope, system is considered as stablizing, and calculates the clearance (formula 1) of ammonia nitrogen and COD.Device intake pump stops water inlet, biofilm carrier disc stops operating, dismantle 4 microbial film examination bars, carry out microbial film in situ test, the fluorescent dyeing of different flooding velocity (corresponding different hydraulic detention time)-Laser Scanning Confocal Microscope observation is obtained microbial film inner different thickness place microorganism Extracellular polymers spatial distribution image photo, atomic force microscope obtains mechanics of biomembranes characteristic, the ammonia oxidation bacteria that image software obtains, nitrite nitrogen oxidation bacteria abundance, microelectrode test macro obtains ammonia nitrogen, dissolved oxygen, nitrate nitrogen in microbial film internal mass transfer coefficient and mass transfer dynamics mathematical model, mutually contrast confirmation, and the removal ammonia nitrogen of above-mentioned microbial film in situ test data results and different hydraulic detention time and COD effect are compared, specify the correlationship of biofilm structure and microbial characteristic and the wastewater treatment efficiency represented with pollutants removal rate under different hydraulic detention time condition, disclose the microscopic mechanism that different hydraulic detention time affects biofilm sewage treatment device wastewater treatment efficiency, and obtain optimal water power standing time.
Embodiment 2:
Flow shear is to the Study on Microcosmic Mechanism of biofilm sewage treatment device treatment effect
According to current bibliographical information, the flow shear that microbial film can tolerate is within the scope of 0 ~ 2Pa.Adopt the device described in example 1 and sewage, keep biofilm sewage treatment device flow rate pump constant, under the optimal water power standing time condition that example 1 is determined, by changing the output of the pulse power, change the rotating speed of rotating shaft, obtain different biofilm surface flow shear condition.Wherein under different rotating speeds condition, biofilm carrier disc different radiuses of rotation place current calculate according to calculation formula (2) biomembranous shearing force.
Wherein ρ is fluid density (kg/m
3), r is the radius (m) of sampling place, and υ is liquid movement viscosity (m
2/ s), ω is circular frequency (r/s), τ is shearing force (Pa).Under different flow shear condition, test organisms film sewage treatment device wastewater treatment efficiency, and keep biofilm structure to carry out in situ test to Biofilm characteristics, concrete steps are as follows:
Under rotating speed is 4rpm condition, when in water outlet, ammonia nitrogen concentration and the daily variation of COD concentration are in ± 5% scope, microbial film system reaches stable, pull down 4 microbial films examination bars, carry out polysaccharide that microbial film Extracellular polymers includes respectively, protein fluorescence dyeing-Laser Scanning Confocal Microscope observation, the test of biofilm microorganisms bonding strength atomic force microscope, microbial film ammonia oxidation bacteria and nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-confocal microscopy, microbial film inside ammonia nitrogen, dissolved oxygen mass transfer process microelectrode test.
After microbial film examination bar has been dismantled, immediately biofilm carrier disc is reinstalled home position, reopen intake pump, go out water pump and the pulse power, change the output of the pulse power, making to change biofilm carrier disc rotation speed is 8rpm, when in water outlet, ammonia nitrogen concentration and the daily variation of COD concentration are in ± 5% scope, microbial film system reaches stable, pollutants removal rate is calculated according to formula (1), pull down 4 microbial film examination bars again, carry out microbial film in situ test, repeat above-mentioned steps, change the output of the pulse power successively, making to change biofilm carrier disc rotation speed is 12rpm, 20rpm, 28rpm, 40rpm, 52rpm, 60rpm, measure the microbial film system wastewater pollutants ammonia nitrogen of steady state, COD clearance, and complete the polysaccharide that microbial film Extracellular polymers includes, protein fluorescence dyeing-Laser Scanning Confocal Microscope observation, biofilm microorganisms bonding strength atomic force microscope is tested, microbial film ammonia oxidation bacteria and nitrite nitrogen oxidation bacteria and total bacterial fluorescence in situ hybridization-confocal microscopy, the inner ammonia nitrogen of microbial film, dissolved oxygen mass transfer process microelectrode is tested, specify the influential effect of flow shear to biofilm sewage treatment device wastewater treatment efficiency, flow shear is disclosed to the microscopic mechanism of biofilm sewage treatment device wastewater treatment efficiency influence in conjunction with microbial film in situ test result.
Claims (7)
1. a biofilm sewage treatment device, is characterized in that, comprises spill tank (1), water inlet system, effluent collection system and computer (19);
Described spill tank (1) is made up of horizontal semicircular cylinder and the rectangular plate that is individually fixed in semicircular cylinder two ends, the opening of rectangular plate upper limb and semicircular cylinder is positioned at same plane, rectangular plate tall and big in the radius of semicircular cylinder, rotating shaft (3) is provided with in the axial location of semicircular cylinder, the two ends of rotating shaft (3) are connected with two pieces of rectangular plates respectively by bearing (14), one end of rotating shaft (3) is connected with the output shaft of the synchronous machine (18) be fixed on this end rectangular plate, synchronous machine (18) is driven by the pulse power (17), rotating shaft (3) is coaxially fixed with the biofilm carrier disc (2) of some circles, the positive and negative card of biofilm carrier disc (2) radially has 3-5 bar rectangular recess (15) randomly, in rectangular recess (15), the seamless microbial film being embedded with material identical with biofilm carrier disc tries bar (16), the card of microbial film examination bar (16) outside surface and biofilm carrier disc (2) is in same plane,
In two pieces of rectangular plates of spill tank (1), one piece has prosopyle (6), another block has posticum (7), spill tank (1) bottom has sewage evacuation port (8);
Described water inlet system comprises suction culvert (10), intake pump (11) and the water collecting basin (9) for holding treatment sewage, and described effluent collection system comprises water pump (12), outlet pipeline (13) and the storage pool (20) for holding the rear sewage of process, the water-in of intake pump (11) connects water collecting basin (9) by suction culvert (10), the water outlet of intake pump (11) connects the prosopyle (6) of spill tank (1), the posticum (7) of spill tank (1) is connected with the water-in going out water pump (12), the water outlet going out water pump (12) connects storage pool (20) by outlet pipeline (13), ammonia nitrogen online testing device (4) and COD online testing device (5) is equipped with in water collecting basin (9) with storage pool (20), ammonia nitrogen online testing device (4) is connected with computer (19) respectively with COD online testing device (5).
2. biofilm sewage treatment device according to claim 1, is characterized in that, described biofilm carrier disc (2) thickness is 0.5 ~ 2cm, and described rectangular recess (15) degree of depth is 1 ~ 3mm.
3. biofilm sewage treatment device according to claim 1, is characterized in that, the length of described rectangular recess (15) is 85% ~ 100%, long-width ratio 10:1 ~ 10:2 of biofilm carrier disc (2) radius.
4. biofilm sewage treatment device according to claim 1, is characterized in that, described biofilm carrier disc (2) radius is spill tank radius 90% ~ 95%.
5. biofilm sewage treatment device according to claim 1, is characterized in that, described prosopyle (6) and posticum (7) are positioned at sustained height, and symmetry is divided into the axial plane of symmetry both sides of spill tank (1) semicircular cylinder.
6. biofilm sewage treatment device according to claim 1, is characterized in that, rotating speed real-time continuous within the scope of 1-300rpm of described rotating shaft (3) is adjustable.
7. the device of application as described in any one of claim 1-6 carries out the method for microbial film in situ test, it is characterized in that, comprises the steps:
1) intake pump (11) is opened, sewage is continuously pumped into spill tank (1) by water collecting basin (9), unbalanced pulse power supply (17) drives synchronous machine, drive rotating shaft (3) with 1 ~ 25rpm fixed-axis rotation, open out water pump (12), the sewage after process is pumped into storage pool (20) by spill tank (1);
2) 35% ~ 40% of spill tank (1) interior liquid level submergence biofilm carrier disc area is made; Ammonia nitrogen online testing device (4) in water collecting basin (9) and storage pool (20) and COD online testing device (5) are tested and are intake and water outlet ammonia nitrogen and COD concentration for all every 24 hours, after testing, drain the waste water in storage pool at every turn; When the water outlet ammonia nitrogens of continuous 3 ~ 5 days and COD concentration standard difference be not in ± 5% scope, device effect of disposing of sewage reaches stable, close intake pump (11), go out water pump (12) and the pulse power (17), biofilm carrier disc (2) is stopped operating, meet microbial film examination bar (16) of testing requirement quantity under dismounting, carry out four groups of microbial film in situ tests:
First group: carry out polysaccharide that microbial film Extracellular polymers includes, protein fluorescence dyeing-Laser Scanning Confocal Microscope observation, obtain microbial film inner different thickness place microorganism Extracellular polymers spatial distribution image photo;
Second group: rap microbial film strip surface microbial film by probe under being placed in atomic force microscope and obtain mechanics of biomembranes characteristic and analyze biofilm microorganisms bonding strength;
3rd group: by microbial film strip surface microbial film through fluorescence in situ hybridization-confocal microscopy, obtain microbial film different thickness place ammonia oxidation bacteria, nitrite nitrogen oxidation bacteria and total bacterium image photo, the ratio that calculating ammonia oxidation bacteria, nitrite nitrogen oxidation bacteria account for total bacterium carries out enrichment analysis;
4th group: be placed in microelectrode test macro, obtain ammonia nitrogen, dissolved oxygen, nitrate nitrogen in microbial film internal mass transfer coefficient and mass transfer dynamics mathematical model;
3) biofilm carrier disc is resetted, reopen intake pump (11), go out water pump (12) and the pulse power (17), change amount of inlet water or change rotating shaft rotating speed, continuing step 2);
4) repeating step 3) until the different flooding quantity of experimental design and the test of different rotating speeds Parameter Conditions all complete;
5) sewage in the result of each microbial film in situ test and this secondary pollutant film sewage treatment device is removed ammonia nitrogen and COD concentration is compared, obtain the correlationship of biofilm structure and microbial characteristic and wastewater treatment efficiency.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110017647A1 (en) * | 2007-11-14 | 2011-01-27 | Degremont | Installation for the treatment of wastewaters and biological disc for such an installation |
| CN103808284A (en) * | 2014-03-04 | 2014-05-21 | 中国科学院重庆绿色智能技术研究院 | Device and method for in-situ detection of internal spatial structure of wastewater treatment biological membrane |
| CN103865757A (en) * | 2014-03-20 | 2014-06-18 | 中国农业大学 | Drip irrigation emitter epiorganism membrane simulation culture device and application thereof |
| CN205076882U (en) * | 2015-09-02 | 2016-03-09 | 浙江大学 | Biological membrane sewage treatment device |
-
2015
- 2015-09-02 CN CN201510556183.XA patent/CN105060464B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110017647A1 (en) * | 2007-11-14 | 2011-01-27 | Degremont | Installation for the treatment of wastewaters and biological disc for such an installation |
| CN103808284A (en) * | 2014-03-04 | 2014-05-21 | 中国科学院重庆绿色智能技术研究院 | Device and method for in-situ detection of internal spatial structure of wastewater treatment biological membrane |
| CN103865757A (en) * | 2014-03-20 | 2014-06-18 | 中国农业大学 | Drip irrigation emitter epiorganism membrane simulation culture device and application thereof |
| CN205076882U (en) * | 2015-09-02 | 2016-03-09 | 浙江大学 | Biological membrane sewage treatment device |
Non-Patent Citations (1)
| Title |
|---|
| 杨蓉等: "生物转盘系统中短程硝化的实现", 《环境科学与技术》 * |
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| CN105758921A (en) * | 2016-03-31 | 2016-07-13 | 中国科学院重庆绿色智能技术研究院 | Device and method for achieving accurate in-situ monitoring of dynamic changes of substance concentration in biomembrane of rotary-type biomembrane reactor |
| CN105784943A (en) * | 2016-03-31 | 2016-07-20 | 中国科学院重庆绿色智能技术研究院 | Device and method for simulating operation state of rotary type bio-membrane reactor |
| CN105784943B (en) * | 2016-03-31 | 2018-03-27 | 中国科学院重庆绿色智能技术研究院 | A kind of device and method for simulating rotary biofilm reactor running status |
| CN105758921B (en) * | 2016-03-31 | 2019-01-01 | 中国科学院重庆绿色智能技术研究院 | A kind of method of material concentration dynamic change in quasi- in-situ monitoring biomembrane |
| CN105713832A (en) * | 2016-04-13 | 2016-06-29 | 浙江大学 | Temperature-controllable biological membrane culture device for non-cyclical water supply pipeline and use method of biological membrane culture device |
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| CN105928982B (en) * | 2016-05-17 | 2018-07-17 | 中国科学院生态环境研究中心 | A kind of alternate biomembrane microenvironment detection device of gas-liquid two-phase |
| CN106018481B (en) * | 2016-05-17 | 2018-09-21 | 中国科学院生态环境研究中心 | A kind of alternate biomembrane microenvironment detection method of gas-liquid two-phase |
| CN109091982A (en) * | 2018-09-04 | 2018-12-28 | 孙勇 | A kind of industrial waste gas processing method |
| CN109091982B (en) * | 2018-09-04 | 2021-04-16 | 深圳市天浩洋环保股份有限公司 | Industrial waste gas treatment method |
| CN109489568A (en) * | 2018-09-30 | 2019-03-19 | 南京大学 | A kind of auxiliary device and measuring method of on-line determination Wastewater treatment filling material biofilm thickness |
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