CN114099390B - Mask liquid containing mesona chinensis benth extract and preparation method thereof - Google Patents
Mask liquid containing mesona chinensis benth extract and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a mask liquid containing mesona chinensis benth extract and a preparation method thereof, wherein the technical scheme comprises 10-15 parts of mesona chinensis benth extract, 6-10 parts of selfheal extract, 5-8 parts of honeysuckle extract, 8-9 parts of kaempferia galangal extract, 5-8 parts of rhizoma anemarrhenae extract, 3-4 parts of compound amino acid, 0.2-0.3 part of carboxymethyl glucan, 0.1-0.2 part of hyaluronic acid, 0.1-0.2 part of allantoin, 1-1.5 parts of propylene glycol, 1-1.5 parts of butanediol, 2-4 parts of glycerol, 0.1-0.2 part of phenoxyethanol and 70-80 parts of water; the mask liquid containing the mesona chinensis benth extract has the functions of diminishing inflammation, inhibiting bacteria, resisting oxidation and activating human keratinocytes, and also has the functions of moisturizing; the mask liquid containing the mesona chinensis benth extract has no irritation to skin, is easy to clean and has no residue, and can not cause skin redness, swelling and stress and poisoning after long-term use, thus being suitable for various people to use; belongs to the technical field of cosmetics.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a mask liquid containing mesona chinensis benth extract and a preparation method thereof.
Background
In recent years, due to the increasing pressure of life and work of people, irregular diet and work and external air pollution are easy to cause accelerated aging, sensitive damage and aggravation of dark circles of facial skin. Many females and even males are focusing on the maintenance of their own skin, but with the improvement of the living standard, the requirements of people on skin care products are increasing. In a plurality of daily skin care products, the facial mask is easy and convenient to use, is easy to clean after being used, and is popular with various consumers.
The facial mask is a skin care product applied on the face, and is essentially a non-woven fabric face soaked with facial mask liquid, and has the functions of moisturizing, activating skin, whitening, resisting aging and the like. When the facial mask is used, the facial mask is lightly applied on the cleaned face for 15-30 minutes, and the facial cuticle is softened by the active ingredients contained in the facial mask, so that skin pores are expanded, nutrition in the facial mask can be absorbed by facial skin, the skin is helped to restore the moisture and elasticity of the surface, the skin temperature is improved, the blood circulation is accelerated, the metabolism is promoted, and redundant grease and metabolic wastes in the surface cells of the skin are removed.
The existing facial masks sold on the market mainly pay attention to the effects of cleaning, moisturizing, whitening, anti-wrinkle and repairing, but a large amount of thickening agents such as polyacrylamide, acrylic acid and the like are added in order to increase the viscosity of the facial mask essence, and the nipagin Jin Yibing ester, methyl chloroisoxazolinone, quaternary ammonium salt-15 and the like are added in order to prevent the facial mask from being polluted by bacteria and deteriorating in the preservation process, so that the facial mask has certain cytotoxicity to skin, and can cause skin inflammation, redness and even poisoning after long-term use.
The Chinese medicinal extract is extracted and separated from natural plants, and contains flavonoid, polyphenol, alkaloid and micromolecular glycoside components with the effects of resisting oxidation, inhibiting bacteria, relieving allergy and the like, has no obvious side effect, and can not irritate skin due to long-term use. Therefore, the facial mask containing natural extract components is welcomed by the masses of consumers with safety, effectiveness, mild effect and no stimulation.
Disclosure of Invention
Accordingly, the invention aims to provide a mask liquid containing mesona chinensis benth extract and a preparation method thereof.
The invention takes mask liquid containing mesona chinensis benth extract as an experimental object, and performs antibacterial activity experiments, DPPH free radical scavenging experiments, tyrosinase inhibition capability experiments and proliferation activity experiments on HaCaT cells (keratinocytes). The result shows that the mask liquid containing the mesona chinensis benth extract can effectively inhibit staphylococcus aureus, escherichia coli, bacillus thuringiensis and bacillus subtilis, has extremely high DPPH free radical clearance rate and tyrosinase inhibition rate, can promote HaCaT cell proliferation, and has a certain skin repair promoting effect. The human body skin patch test result and the trial (4 weeks) feedback evaluation of the mask liquid show that the mask liquid containing the mesona chinensis benth extract also has the functions of moisturizing and moisturizing, has no irritation to skin, is easy to clean and has no residue, and can not cause skin redness, swelling, stress and poisoning after long-term use, thus being suitable for various people.
Therefore, the invention provides a mask liquid containing mesona chinensis benth extract, which comprises the following raw materials in parts by weight:
15 parts of mesona chinensis benth extract, 9 parts of selfheal extract, 5 parts of honeysuckle extract, 8 parts of kaempferia galangal extract, 5 parts of rhizoma anemarrhenae extract, 3 parts of compound amino acid, 0.2 part of carboxymethyl glucan, 0.1 part of hyaluronic acid, 0.1 part of allantoin, 1 part of propylene glycol, 1 part of butanediol, 2 parts of glycerol, 0.1 part of phenoxyethanol and 80 parts of water.
Further, the mask liquid containing the mesona chinensis benth extract is composed of the following raw materials in parts by weight:
15 parts of mesona chinensis benth extract, 9 parts of selfheal extract, 5 parts of honeysuckle extract, 8 parts of kaempferia galangal extract, 5 parts of rhizoma anemarrhenae extract, 3 parts of compound amino acid, 0.2 part of carboxymethyl glucan, 0.1 part of hyaluronic acid, 0.1 part of allantoin, 1 part of propylene glycol, 1 part of butanediol, 2 parts of glycerol, 0.1 part of phenoxyethanol and 80 parts of water.
Further, the preparation method of the mesona chinensis benth extract comprises the following steps: pulverizing dried mesona chinensis benth, sieving with a 40-100 mesh sieve, adding 50% ethanol which is 20 times the weight of the mesona chinensis benth coarse powder, performing ultrasonic-assisted cold soaking, performing ultrasonic power 350W, performing cold soaking for 2 hours, performing heating reflux extraction for 3 times, filtering, combining filtrates, concentrating under reduced pressure, loading the filtrate onto an AB-8 macroporous resin column, sequentially eluting according to water, 50% ethanol solution and 95% ethanol solution, collecting 50% eluting part, and concentrating under reduced pressure to recover ethanol to obtain the mesona chinensis benth extract.
Further, the preparation method of the selfheal extracting solution, the honeysuckle extracting solution, the kaempferia galangal extracting solution and the rhizoma anemarrhenae extracting solution comprises the following steps: pulverizing dried selfheal, honeysuckle, rhizoma kaempferiae and rhizoma anemarrhenae, sieving with a 40-100 mesh sieve respectively, adding extraction solvents respectively, soaking for 0-12 h, respectively carrying out reflux extraction, removing insoluble substances, and drying to obtain the extracting solution of the selfheal, the honeysuckle, the rhizoma kaempferiae and the rhizoma anemarrhenae.
Further, the preparation method of the mask liquid containing mesona chinensis benth extract liquid comprises the following steps:
(1) Adding water, compound amino acid and allantoin into a stirring pot, heating to 50deg.C, homogenizing for 8min, stirring until all the raw materials are completely dissolved to obtain a clear mixed solution A, and taking out for use;
(2) Uniformly mixing propylene glycol, butanediol, glycerol, carboxymethyl dextran and hyaluronic acid, homogenizing at normal temperature for 5min to obtain mixed solution B, and taking out for later use;
(3) Sequentially adding the clear mixed solution A, the mixed solution B, the mesona chinensis extracting solution, the selfheal extracting solution, the honeysuckle extracting solution, the rhizoma kaempferiae extracting solution, the rhizoma anemarrhenae extracting solution and the phenoxyethanol, and homogenizing for 25min to obtain the mask solution containing the mesona chinensis extracting solution.
Further, the mask liquid containing the mesona chinensis benth extract comprises the following components in percentage by mass: arginine 10%, leucine 10%, isoleucine 10%, valine 10%, methionine 10%, threonine 10%, phenylalanine 10%, aspartic acid 10%, lysine 10%, tryptophan 10%.
Furthermore, the mask liquid containing the mesona chinensis benth extract liquid is matched with a non-woven fabric mask to be used, each mask contains 25g of the mask liquid, the mask is cleaned before use, and the mask is unfolded and lightly attached to the face during use.
The invention has the following advantages:
(1) The mask liquid containing the mesona chinensis benth extract liquid has a broad-spectrum sterilization effect, can reduce the use of preservatives in raw materials to a certain extent, has good antioxidant and whitening activities, can delay skin aging, whiten skin, keep the skin moist degree, and can promote HaCaT cell proliferation and repair the skin to a certain extent.
(2) The mask liquid containing the mesona chinensis benth extract liquid has no irritation to skin, is easy to clean and has no residue, and can not cause skin redness and swelling stress and poisoning after long-term use, thus being suitable for various people.
(3) The mask liquid containing the mesona chinensis benth extract liquid is simple in preparation method, controllable in condition and stable in process, and is beneficial to popularization and production and application.
Drawings
FIG. 1 shows a graph of proliferation of HaCaT cells with mask solution containing mesona chinensis extract.
Description of the embodiments
In order to make the objects, features and advantages of the present invention more obvious and understandable, the technical solutions of the embodiments of the present invention will be clearly and completely described below.
The raw materials used in the embodiment of the invention are all sold in the market or self-made.
Example 1: preparation of mask liquid containing mesona chinensis benth extract
The mesona chinensis composition in the embodiment of the invention comprises the following raw materials in parts by weight: 15 parts of mesona chinensis benth extract, 9 parts of selfheal extract, 5 parts of honeysuckle extract, 8 parts of kaempferia galangal extract, 5 parts of rhizoma anemarrhenae extract, 3 parts of compound amino acid, 0.2 part of carboxymethyl glucan, 0.1 part of hyaluronic acid, 0.1 part of allantoin, 1 part of propylene glycol, 1 part of butanediol, 2 parts of glycerol, 0.1 part of phenoxyethanol and 80 parts of water.
The preparation method comprises the following steps:
(1) Adding water, compound amino acid and allantoin into a stirring pot, heating to 50deg.C, homogenizing for 8min, stirring until all the raw materials are completely dissolved to obtain a clear mixed solution A, and taking out for use;
(2) Uniformly mixing propylene glycol, butanediol, glycerol, carboxymethyl dextran and hyaluronic acid, homogenizing at normal temperature for 5min to obtain mixed solution B, and taking out for later use;
(3) Sequentially adding the clear mixed solution A, the mixed solution B, the mesona chinensis extracting solution, the selfheal extracting solution, the honeysuckle extracting solution, the rhizoma kaempferiae extracting solution, the rhizoma anemarrhenae extracting solution and the phenoxyethanol, and homogenizing for 25min to obtain the mask solution containing the mesona chinensis extracting solution.
Example 2: preparation of mask liquid containing mesona chinensis benth extract
The mesona chinensis composition in the embodiment of the invention comprises the following raw materials in parts by weight: 10 parts of mesona chinensis benth extract, 6 parts of selfheal extract, 8 parts of honeysuckle extract, 9 parts of kaempferia galangal extract, 8 parts of rhizoma anemarrhenae extract, 4 parts of compound amino acid, 0.3 part of carboxymethyl glucan, 0.1 part of hyaluronic acid, 0.1 part of allantoin, 1 part of propylene glycol, 2 parts of butanediol, 4 parts of glycerol, 0.1 part of phenoxyethanol and 70 parts of water.
The preparation method is described in example 1.
Example 3: preparation of mask liquid containing mesona chinensis benth extract
The mesona chinensis composition in the embodiment of the invention comprises the following raw materials in parts by weight: 10 parts of mesona chinensis benth extract, 10 parts of selfheal extract, 6 parts of honeysuckle extract, 9 parts of kaempferia galangal extract, 6 parts of rhizoma anemarrhenae extract, 4 parts of compound amino acid, 0.3 part of carboxymethyl glucan, 0.2 part of hyaluronic acid, 0.2 part of allantoin, 1.5 parts of propylene glycol, 1.5 parts of butanediol, 2 parts of glycerol, 0.2 part of phenoxyethanol and 70 parts of water.
The preparation method is described in example 1.
Comparative example 1: preparation of mask liquid containing mesona chinensis benth extract
The mesona chinensis composition in the embodiment of the invention comprises the following raw materials in parts by weight: 15 parts of mesona chinensis benth extract, 5 parts of honeysuckle extract, 8 parts of kaempferia galangal extract, 5 parts of rhizoma anemarrhenae extract, 3 parts of compound amino acid, 0.2 part of carboxymethyl dextran, 0.1 part of hyaluronic acid, 0.1 part of allantoin, 1 part of propylene glycol, 1 part of butanediol, 2 parts of glycerol, 0.1 part of phenoxyethanol and 75 parts of water.
The preparation method is described in example 1.
Comparative example 2: preparation of mask liquid containing mesona chinensis benth extract
The mesona chinensis composition in the embodiment of the invention comprises the following raw materials in parts by weight: 15 parts of mesona chinensis benth extract, 9 parts of selfheal extract, 5 parts of honeysuckle extract, 5 parts of rhizoma anemarrhenae extract, 3 parts of compound amino acid, 0.2 part of carboxymethyl glucan, 0.1 part of hyaluronic acid, 0.1 part of allantoin, 1 part of propylene glycol, 1 part of butanediol, 2 parts of glycerol, 0.1 part of phenoxyethanol and 75 parts of water.
The preparation method is described in example 1.
The compound amino acid described in examples 1-3 and comparative examples 1-2 comprises the following components in percentage by mass: arginine 10%, leucine 10%, isoleucine 10%, valine 10%, methionine 10%, threonine 10%, phenylalanine 10%, aspartic acid 10%, lysine 10%, tryptophan 10%.
The preparation methods of the mesona chinensis benth extract liquid in the examples 1-3 and the comparative examples 1-2 are as follows:
(1) Weighing herba mesonae chinensis 100g, pulverizing with pulverizer, sieving with 60 mesh sieve,
(2) According to the feed liquid ratio 1:20, adding 2L of 50% (v/v) ethanol solution, performing ultrasonic assisted cold soaking, performing ultrasonic power 350W, performing cold soaking for 2h,
(3) Placing the obtained extract and residue in a round bottom flask, connecting the round bottom flask with a condensing device, heating with a constant temperature electrothermal sleeve at 100deg.C, reflux-extracting for 2 hr, repeating extraction for 3 times, collecting extract,
(4) Filtering to remove insoluble substances, mixing the three extractive solutions, concentrating under reduced pressure to about 50mL,
(5) And (3) loading the concentrated solution to an AB-8 macroporous resin column according to the dry weight of the sample to be loaded and the mass ratio of the filler of 1:20, eluting sequentially according to water, 50% ethanol solution and 95% ethanol solution, collecting 50% elution part, and concentrating under reduced pressure to recover ethanol to obtain mesona chinensis benth extract.
Example 4: preparation of selfheal extract
(1) 100g of selfheal is accurately weighed, crushed by a crusher and sieved by a 60-mesh sieve;
(2) According to the feed liquid ratio 1:20, adding 2L of 75% (v/v) ethanol solution, soaking for 8h, and extracting under reflux for 3 times at 80deg.C for 2 h;
(3) Filtering to remove insoluble substances, mixing the extractive solutions, concentrating under reduced pressure, and recovering ethanol to obtain Prunellae Spica extractive solution.
Example 5: preparation of honeysuckle extract
(1) Accurately weighing 100g of honeysuckle, crushing by a crusher, and sieving by a 60-mesh sieve;
(2) According to the feed liquid ratio 1:20, adding 2L of 60% (v/v) ethanol solution, soaking for 8h, and extracting under reflux for 3 times at 80deg.C for 2 h;
(3) Filtering to remove insoluble substances, mixing the extractive solutions, concentrating under reduced pressure to recover ethanol to obtain flos Lonicerae extractive solution.
Example 6: preparation of kaempferia galanga extract
(1) Accurately weighing 100g of rhizoma Kaempferiae, pulverizing with pulverizer, sieving with 60 mesh sieve
(2) According to the feed liquid ratio 1:20, adding 2L of 60% (v/v) ethanol solution, soaking for 8h, and extracting under reflux for 3 times at 80deg.C for 2 h.
(3) Filtering to remove insoluble substances, mixing the extractive solutions, concentrating under reduced pressure, and recovering ethanol to obtain rhizoma Kaempferiae extractive solution.
Example 7: preparation of rhizoma anemarrhenae extract
(1) Accurately weighing 100g of rhizoma anemarrhenae, pulverizing with pulverizer, and sieving with 60 mesh sieve
(2) According to the feed liquid ratio 1:20, adding 2L of 50% (v/v) ethanol solution, soaking for 8h, and extracting under reflux for 3 times at 80deg.C for 2 h.
(3) Filtering to remove insoluble substances, mixing the extractive solutions, concentrating under reduced pressure to recover ethanol to obtain rhizoma anemarrhenae extractive solution.
In order to demonstrate the advantages of the technical solutions provided herein, experimental detection of the technical solutions provided herein is given below.
Example 8 antibacterial test of mask liquid containing mesona chinensis extract
Test sample: mask liquid containing mesona chinensis extract prepared in examples 1 to 3 and comparative examples 1 to 2.
The experimental method comprises the following steps:
(1) Inoculating Staphylococcus aureus, escherichia coli, bacillus thuringiensis and Bacillus subtilis into LB broth, shaking (30 deg.C, 160 rpm) and culturing overnight;
(2) After activation, inoculating the strain into LB liquid in a proportion of 2%, and continuously culturing until about 0.6 of 0D600 nm is reached for later use;
(3) Dividing the culture dishes into 4 groups, sucking 100 mu L of each bacterial liquid by a pipetting gun, uniformly coating the bacterial liquids on LB solid culture mediums of the groups, punching 6 equidistant holes by an oxford cup, respectively adding 50 mu L of mask liquid containing mesona chinensis extract prepared in examples 1-3 and comparative examples 1-2, adding ultrapure water into one hole to serve as a blank group contrast, and standing for culturing in a 37 ℃ incubator;
(4) After the incubator is incubated for 12h hours, the diameter of the inhibition zone is measured and the average value is taken as the final result.
Table 1 results of antibacterial test of mask liquid containing Mesona chinensis extract
Experimental results: as can be seen from Table 1, the mask liquid containing the mesona chinensis extract solution prepared by the invention has good antibacterial activity, and can obviously inhibit staphylococcus aureus, escherichia coli, bacillus thuringiensis and bacillus subtilis, wherein the antibacterial effect of example 1 is optimal. The antibacterial results of comparative examples 1 and 2 show that the lack of the selfheal extract and the kaempferia galangal extract can reduce the inhibition effect of the mask liquid on staphylococcus aureus, escherichia coli, bacillus thuringiensis and bacillus subtilis, which shows that the selfheal extract, the honeysuckle extract, the mesona chinensis extract, the kaempferia galangal extract and the anemarrhena extract can exert synergistic antibacterial effect.
DPPH free radical scavenging ability test of mask liquid containing mesona chinensis extract
Test sample: examples 1-3, comparative examples 1 and 2 prepared a mask solution containing mesona chinensis benth extract as an experimental group, a vitamin C solution of 100. Mu.g/mL as a positive control group, and ultrapure water as a blank control group.
The experimental method comprises the following steps:
(1) Weighing 4mg of DPPH solid medicine, adding 28mL of absolute ethyl alcohol to prepare a DPPH solution, weighing 5mg of vitamin C, adding 50mL of absolute ethyl alcohol to prepare a positive control group solution;
(2) 100 mu L of DPPH solution is added into a 96-well plate, 100 mu L of mask liquid containing mesona chinensis benth extract prepared in examples 1-3 and comparative examples 1-2 is added into each experimental group, 100 mu L of prepared vitamin C solution is added into a positive control group, 100 mu L of ultrapure water is added into a blank control group, and 3 compound holes are formed in each group;
(3) Under the condition of light shielding, reacting for 30min at 25 ℃, then placing the reaction product in an enzyme-labeled instrument, setting the wavelength to 517nm, and measuring an OD value;
(4) Radical clearance calculation formula:
radical clearance = (OD blank-OD sample)/OD blank × 100%
TABLE 2 DPPH radical scavenging Rate of mask liquid containing Mesona chinensis extract
Experimental results: from the experimental results in table 2, it can be seen that the mask solutions prepared in examples 1-3 all have good antioxidant activity, can effectively remove DPPH free radicals, and the removal rate is superior to that of vitamin C. The selfheal extract and the kaempferia galanga extract are removed in comparative examples 1 and 2 respectively, so that the free radical scavenging capacity is reduced, wherein the reduction of comparative example 2 is particularly obvious, and the reduction is reduced by 25.88%. The results show that the selfheal extract and the kaempferia galanga extract play a synergistic role together with other extracts in the composition, and the free radical removal is completed together.
Tyrosinase inhibition capability test of mask liquid containing mesona chinensis benth extract
Test sample: examples 1-3 and comparative examples 1-2 facial mask solutions containing mesona chinensis benth extract were prepared as experimental groups, vitamin C solution of 100. Mu.g/mL was used as positive control group, and ultrapure water was used as blank control group.
The experimental method comprises the following steps:
(1) Dissolving 5mg of tyrosinase in 50mL of PBS solution to prepare 0.5 mg/mL of solution for later use; preparing L-DOPA into 0.5 mM solution with PBS solution for later use;
(2) Sequentially adding 160 mu L of PBS buffer solution and 20 mu L of L-DOPA solution into a 96-well plate, uniformly mixing, then adding 10 mu L of the mask solution prepared in each example into an experimental group, adding 10 mu L of vitamin C solution into a positive control group, and adding 10 mu L of ultrapure water into a blank control group;
(3) 10. Mu.L of tyrosinase solution was added to each well, preincubated at 37℃for 30 minutes, then placed in an microplate reader to set a wavelength of 475nm, OD was measured, and inhibition was calculated.
TABLE 3 tyrosinase inhibition test of mask liquid containing mesona chinensis extract
Experimental results: as shown in the experimental results of Table 3, all of examples 1-3 had strong tyrosinase inhibitory activity, wherein the inhibition rate of example 1 reached 89.69%, which was higher than 85.91% of the positive control. Meanwhile, experiments show that the tyrosine inhibition rate of the mask liquid is not obviously reduced after the selfheal extracting solution is removed, and the tyrosine inhibition effect is obviously reduced after the kaempferia galangal extracting solution is removed. The results demonstrate that the kaempferia galanga extract, mesona chinensis extract, honeysuckle extract and rhizoma anemarrhenae extract together exert the main tyrosinase inhibitory activity in the inventive mask liquid.
Human body skin patch test of mask liquid containing mesona chinensis benth extract
Test sample: mask liquid containing mesona chinensis extract prepared in examples 1-3 and comparative examples 1-2.
The experimental method comprises the following steps:
(1) 50 healthy females are selected, the ages of which are 25-30, and are divided into 5 groups of 10 people each;
(2) Selecting a qualified patch tester, dripping 25 mu L of the mask liquid prepared in the examples 1-10 into the patch tester in a closed patch experiment mode, and sticking the mask liquid on the back of a subject by using a special adhesive tape;
(3) After 24 hours of application, the adhesive tape layer of the patch tester was removed, the skin appearance of the applied back was observed after 30 minutes, and the skin of the applied back was observed again after 12, 24, 36, 48 hours, and the results were recorded according to the skin reaction classification standard in the cosmetic hygiene Specification.
Experimental results: the test results of the human skin patch show that: each group of subjects observe skin reactions in 12, 24, 36 and 48 hours through the spot-stick test of the mask liquid containing the mesona chinensis benth extract prepared in examples 1-3 and comparative examples 1-2, and the mask liquid containing the mesona chinensis benth extract is safe to use.
Trial evaluation of mask liquid containing mesona chinensis extract for 4 weeks
Test sample: mask liquid containing mesona chinensis extract prepared in examples 1-3 and comparative examples 1-2.
The experimental method comprises the following steps:
(1) 50 healthy females are selected, the ages of which are 25-30, and are divided into 5 groups of 10 people each;
(2) After all subjects clean the face, the face mask soaked by the face mask liquid is lightly applied to the face, and the face mask is used for 3 times per week for 20min for 4 weeks;
(3) After 4 weeks of use, evaluating the moisturizing effect, the irritation, the smell, the comfort and the overall effect of the mask liquid containing the mesona chinensis benth extract;
(4) The evaluation method comprises the following steps:
(1) the water supplementing effect is 0-10 minutes, and the higher the score is, the better the representative effect is;
(2) the irritation is 0-10 minutes, and the higher the score is, the smaller the irritation is represented;
(3) the smell is 0-10 minutes, and the higher the score is, the more pleasant the smell is represented;
(4) the comfort is 0-10 minutes, and the higher the score is, the more comfortable the user is represented;
(5) the overall effect is evaluated according to 0-10 points, and the higher the point value is, the better the overall effect is represented;
all the above results were averaged.
Table 4 trial (4 weeks) evaluation of mask liquid containing mesona chinensis extract
Experimental results: as shown in Table 4, the mask liquid containing mesona chinensis benth extract prepared by the invention has good moisturizing effect, no obvious smell, mild skin and good overall evaluation effect, can not cause skin redness, swelling stress and poisoning after long-term use, and is suitable for various people.
Growth experiment of Mesona chinensis Benth extract-containing mask liquid on HaCaT cells
Test sample: mask liquid containing mesona chinensis extract prepared in examples 1-3 and comparative examples 1-2.
The experimental method comprises the following steps:
(1) Diluting the mask liquid prepared in each example 1-3 and comparative example 1-2 to 1, 2, 5, 10, 20, 50 and 100 mug/mL by using a DEME culture medium;
(2) Taking HaCaT cells in logarithmic growth phase, inoculating 1x104 cells per well into a 96-well plate, culturing at 37 ℃ under 5% CO2 for 24h, wherein the volume of each well is 100 mu L;
(3) Discarding cell plate supernatant, adding 100 mu L of the mask liquid prepared in examples 1-3 and comparative examples 1-2 with different concentrations, and continuously culturing for 20 hours;
(4) mu.L MTT (5 mg/ml) was added to each well and the cell plates were incubated for an additional 4 hours;
(5) The cell plate supernatant was discarded, 100. Mu.L of DMSO was added to each well, the shaking was uniform, the wavelength was set at 570nm in an microplate reader, the OD was measured, and the cell viability was calculated.
Experimental results: as shown in FIG. 1, the mask solutions prepared in examples 1-3 exhibited proliferation promoting effect on HaCaT cells with increasing concentration, and cell viability remained substantially unchanged after the concentration was greater than 20 mg/mL. The embodiment shows that the mask liquid containing the mesona chinensis benth extract has the function of promoting the proliferation of HaCaT cells.
Claims (4)
1. The mask liquid containing the mesona chinensis benth extract is characterized by comprising the following raw materials in parts by weight: 10-15 parts of mesona chinensis benth extract, 6-10 parts of selfheal extract, 5-8 parts of honeysuckle extract, 8-9 parts of kaempferia galanga extract, 5-8 parts of rhizoma anemarrhenae extract, 3-4 parts of compound amino acid, 0.2-0.3 part of carboxymethyl glucan, 0.1-0.2 part of hyaluronic acid, 0.1-0.2 part of allantoin, 1-1.5 parts of propylene glycol, 1-1.5 parts of butanediol, 2-4 parts of glycerol, 0.1-0.2 part of phenoxyethanol and 70-80 parts of water;
the preparation method of the mesona chinensis benth extract comprises the following steps: pulverizing dried mesona chinensis benth, sieving with a 40-100 mesh sieve, adding 50% ethanol which is 20 times the weight of the coarse powder of the mesona chinensis benth, carrying out ultrasonic-assisted cold soaking for 2 hours, heating and reflux-extracting for 3 times, filtering, combining filtrates, concentrating under reduced pressure, loading the filtrate onto an AB-8 macroporous resin column, eluting sequentially according to water, 50% ethanol solution and 95% ethanol solution, collecting 50% eluting part, concentrating under reduced pressure, and recovering ethanol to obtain mesona chinensis benth extract;
the condition of ultrasonic auxiliary cold soaking is that ultrasonic power is 350W and temperature is 25 ℃;
the preparation method of the selfheal, honeysuckle, rhizoma kaempferiae and rhizoma anemarrhenae extracting solution comprises the following steps: pulverizing dried selfheal, honeysuckle, rhizoma kaempferiae and rhizoma anemarrhenae, sieving with a 40-100 mesh sieve, respectively adding extraction solvents, soaking for 8 hours, performing reflux extraction, removing insoluble substances, concentrating under reduced pressure, and recovering ethanol to obtain the extracting solutions of the selfheal, the honeysuckle, the rhizoma kaempferiae and the rhizoma anemarrhenae.
2. The mask liquid containing the mesona chinensis extracting solution according to claim 1, wherein the mask liquid containing the mesona chinensis extracting solution is composed of the following raw materials in parts by weight: 15 parts of mesona chinensis benth extract, 9 parts of selfheal extract, 5 parts of honeysuckle extract, 8 parts of kaempferia galangal extract, 5 parts of rhizoma anemarrhenae extract, 3 parts of compound amino acid, 0.2 part of carboxymethyl glucan, 0.1 part of hyaluronic acid, 0.1 part of allantoin, 1 part of propylene glycol, 1 part of butanediol, 2 parts of glycerol, 0.1 part of phenoxyethanol and 80 parts of water.
3. The mask liquid containing mesona chinensis benth extract according to claim 1 or 2, wherein the compound amino acid comprises the following components in percentage by mass: arginine 10%, leucine 10%, isoleucine 10%, valine 10%, methionine 10%, threonine 10%, phenylalanine 10%, aspartic acid 10%, lysine 10%, tryptophan 10%.
4. A method for preparing the mask liquid containing mesona chinensis benth extract of claim 1, which is characterized by comprising the following steps:
(1) Adding water, compound amino acid and allantoin into a stirring pot, heating to 50deg.C, homogenizing for 8min, stirring until all the raw materials are completely dissolved to obtain a clear mixed solution A, and taking out for use;
(2) Uniformly mixing propylene glycol, butanediol, glycerol, carboxymethyl dextran and hyaluronic acid, homogenizing at normal temperature for 5min to obtain mixed solution B, and taking out for later use;
(3) Sequentially adding the clear mixed solution A, the mixed solution B, the mesona chinensis extracting solution, the selfheal extracting solution, the honeysuckle extracting solution, the rhizoma kaempferiae extracting solution, the rhizoma anemarrhenae extracting solution and the phenoxyethanol, and homogenizing for 25min to obtain the mask solution containing the mesona chinensis extracting solution.
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| CN105168078A (en) * | 2015-08-14 | 2015-12-23 | 王德君 | Traditional Chinese medicine facial mask, preparation method and application method |
| CN105748391A (en) * | 2016-03-10 | 2016-07-13 | 徐杰 | Multi-effect beauty mask and preparation method thereof |
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| CN107233263A (en) * | 2017-08-04 | 2017-10-10 | 遵义医学院 | A kind of Radix Codonopsis sleep mask and preparation method thereof |
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