CN114098140B - Additive for electronic atomized liquid and preparation method thereof - Google Patents

Additive for electronic atomized liquid and preparation method thereof Download PDF

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CN114098140B
CN114098140B CN202111420252.6A CN202111420252A CN114098140B CN 114098140 B CN114098140 B CN 114098140B CN 202111420252 A CN202111420252 A CN 202111420252A CN 114098140 B CN114098140 B CN 114098140B
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extract
additive
extracting
extracting solution
parts
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CN114098140A (en
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邹阳
邹军
刘梅森
陈慧
唐忠月
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Shenzhen Zinwi Biotech Co Ltd
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Shenzhen Zinwi Biotech Co Ltd
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    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • A24B15/16—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes
    • A24B15/167—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes in liquid or vaporisable form, e.g. liquid compositions for electronic cigarettes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
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    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815—Lycium (desert-thorn)
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    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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Abstract

The invention provides an additive for electronic atomization liquid and a preparation method thereof. The additive can be used for preparing electronic atomized liquid, and is specifically prepared by mixing the additive with propylene glycol, glycerol and the like. The additive is beneficial to slowing down the volatility of the electronic atomized liquid, has a good inhibition effect on oral bacteria, is beneficial to oral health care, and has good market popularization value.

Description

Additive for electronic atomized liquid and preparation method thereof
Technical Field
The invention relates to the technical field of electronic cigarettes, in particular to an additive for electronic atomization liquid and a preparation method thereof.
Background
The electronic cigarette does not contain harmful substances such as tar, carbon monoxide and the like, has little harm to the body of a consumer, has the appearance, smoke and feeling similar to that of a cigarette, and is an electronic product simulating the cigarette. The electronic cigarette mainly comprises a lithium battery, an atomizer and electronic atomized liquid, wherein the lithium battery atomizes the electronic atomized liquid after supplying power to the atomizer, so that the electronic atomized liquid is converted into mist, and real suction feeling is given to a user. The electronic atomization liquid is a core part of the electronic cigarette, and the taste and quality of the electronic atomization liquid determine the smoking experience of the electronic cigarette.
However, the electronic atomization liquid is volatilized in a large amount after being left for a long time, and the fragrant substances are dissipated therewith, so that the flavor and the quality of the electronic atomization liquid are deteriorated. Therefore, how to reduce the volatility of the electronic atomization liquid has very important significance.
In addition, bacteria are easy to grow in the oral cavity of a human body, and long-term and continuous bacterial growth can cause oral diseases, even diseases such as migraine and the like. If can restrain the breed of oral cavity bacterium in the electron cigarette smoking process, can bring better use experience to people undoubtedly.
Patent CN113475742A discloses a tea-flavored electronic atomized liquid, which is prepared from polyhydric alcohol, tea leaf raw material, tobacco leaf extract, monoglyceride, sodium polyacrylate, sodium trimetaphosphate, tobacco essence and the like. Although the electronic atomization liquid can slow down the volatilization speed to a certain extent, the electronic atomization liquid does not have any inhibition effect on oral bacteria, and cannot meet the high-quality requirement of consumers on electronic cigarettes.
Disclosure of Invention
The invention aims to provide an additive for electronic atomization liquid and a preparation method thereof, which are beneficial to slowing down the volatility of the electronic atomization liquid, have a good inhibition effect on oral bacteria and are beneficial to oral health care.
In order to achieve the purpose, the invention is realized by the following scheme:
a preparation method of an additive for electronic atomization liquid comprises the following specific steps:
(1) Uniformly dispersing testa Tritici in water, homogenizing under high pressure to obtain testa Tritici slurry, and supercritical CO 2 Extracting, performing enzymolysis by using compound enzyme, inactivating enzyme, centrifuging to obtain enzymolysis liquid, and performing spray drying to obtain an extract A;
(2) Respectively crushing the corydalis tuber, the subprostrate sophora and the cortex lycii radicis to 200-300 meshes, mixing to obtain mixed powder, and then sequentially extracting the mixed powder by using distilled water, absolute ethyl alcohol and petroleum ether to obtain an extract B;
(3) And (3) adding glucosyl stevioside and the extract A obtained in the step (1) into water, stirring until the glucosyl stevioside and the extract A are completely dissolved, then adding the extract B obtained in the step (2), uniformly dispersing by ultrasonic waves, and performing spray drying to obtain the additive.
Preferably, in step (1), supercritical CO 2 The specific method for extraction comprises the following steps: adding 15-25 parts of wheat bran slurry into an extraction kettle, and introducing supercritical CO 2 And 2-3 parts of methanol, wherein the pressure is 30-35 MPa, and the flow rate of a mixed fluid obtained after gas mixing is 25-30L/h.
Preferably, in the step (1), the mass ratio of the wheat bran, the water and the complex enzyme is 20-30: 160-180: 0.8 to 1.
Preferably, in the step (1), the process conditions of high-pressure homogenization are as follows: homogenizing for 2-3 times under 100-120 MPa.
Preferably, in step (1), the complex enzyme comprises: 3-5 parts of alpha-amylase, 2-3 parts of neutral protease, 2-3 parts of cellulase and 0.8-1 part of xylanase.
Preferably, in the step (1), the enzymolysis process conditions are as follows: pH = 5-6, temperature 50-60 deg.C, enzymolysis time 45-55 minutes.
Preferably, in the step (1), the spray drying process conditions are as follows: the inlet air temperature is 150-160 ℃, the outlet air temperature is 65-75 ℃, and the compressed air flow is 600-700 mL/h.
Preferably, in the step (2), the mass ratio of the corydalis tuber to the subprostrate sophora root to the cortex lycii radicis is 10-12: 7 to 9:3 to 4.
Preferably, in the step (2), the preparation method of the extract B comprises the following steps:
(2-1) firstly adding 10 parts of mixed powder into 55-65 parts of distilled water, heating and extracting for 2-3 hours at the temperature of 80-90 ℃, and centrifuging to obtain an extracting solution I and residues I;
(2-2) adding the residue I into 45-50 parts of absolute ethyl alcohol, heating and extracting for 3-4 hours at 50-60 ℃, and centrifuging to obtain an extracting solution II and a residue II;
(2-3) adding the residue II into 35-45 parts of petroleum ether, heating and extracting for 2-3 hours at 60-70 ℃, and centrifuging to obtain an extracting solution III;
and (2-4) finally, spray drying the extracting solution I, the extracting solution II and the extracting solution III respectively, and then combining to obtain the extract B.
Preferably, in the step (3), the mass ratio of the glucosyl stevioside, the extract A, the water and the extract B is 15-20: 10-15: 100:5 to 7.
Preferably, in the step (3), the spray drying process conditions are as follows: the air inlet temperature is 155-165 ℃, the air outlet temperature is 60-70 ℃, and the compressed air flow is 500-600 mL/h.
The invention also claims an additive for the electronic atomization liquid obtained by the preparation method and application of the additive in preparation of the electronic atomization liquid.
The invention also claims an electronic atomization liquid which contains the additive.
Preferably, the composition is prepared by mixing the following components in parts by weight: 50 to 60 portions of propylene glycol, 40 to 50 portions of glycerol, 0.5 to 0.8 portion of additive and 1 to 1.5 portions of essence.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention firstly uses wheat bran as a raw material to obtain an extract A, then uses corydalis tuber, subprostrate sophora and cortex lycii radicis as raw materials to obtain an extract B, and finally mixes glucosyl stevioside with the extract A and the extract B to obtain the additive. The additive can be used for preparing electronic atomized liquid, and specifically, the electronic atomized liquid can be obtained by mixing the additive with propylene glycol, glycerol and the like. The additive is beneficial to slowing down the volatility of the electronic atomized liquid, has a good inhibition effect on oral bacteria, is beneficial to oral health care, and has good market popularization value.
(2) The additive mainly comprises three parts, namely glucosyl stevioside, an extract A and an extract B. The glucose-based stevioside has a microcapsule structure and contains a plurality of hydroxyl groups, and hydrogen bond effect is formed by the hydroxyl groups, propylene glycol and glycerol, and the adsorption and wrapping effect of the microcapsule structure can fix the components of the electronic atomized liquid, so that the volatility of the electronic atomized liquid is effectively reduced. The extract A takes water-soluble dietary fiber as a main component, and a large amount of hydroxyl contained in the water-soluble dietary fiber can form hydrogen bond action with propylene glycol, glycerol and the like, so that the fixing action is further strengthened, and the volatility of the electronic atomized liquid is slowed down. The extract B has good inhibition effect on oral bacteria.
(3) According to the invention, partial extracts A and B can be partially embedded by virtue of a microcapsule structure of glucosyl stevioside, and a three-dimensional network structure is formed by the hydrogen bond interaction between the extracts, so that the fixing effect is further strengthened, and the volatility of the electronic atomized liquid is slowed down. In addition, the formation of intermolecular hydrogen bonding enables the system to contain more free bacteriostatic substances, which is beneficial to further improving the bacteriostatic effect.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
A preparation method of an additive for electronic atomization liquid comprises the following specific steps:
(1) Uniformly dispersing 20g of testa Tritici in 180g of water, homogenizing under high pressure to obtain testa Tritici slurry, and supercritical CO 2 Extracting, performing enzymolysis with 0.8g of complex enzyme, inactivating enzyme, centrifuging to obtain enzymolysis solution, and spray drying to obtain extract A;
(2) Respectively crushing 12g of rhizoma corydalis, 7g of subprostrate sophora and 4g of cortex lycii radicis to 200 meshes, mixing to obtain mixed powder, and sequentially extracting the mixed powder by using distilled water, absolute ethyl alcohol and petroleum ether to obtain an extract B;
(3) And then adding 20g of glucosyl stevioside and 10g of the extract A into 100g of water, stirring until the glucosyl stevioside and the extract A are completely dissolved, then adding 7g of the extract B, uniformly dispersing by ultrasonic waves, and carrying out spray drying to obtain the additive.
Wherein, in the step (1), supercritical CO is adopted 2 The extraction method comprises the following steps: adding 15g of wheat bran slurry into an extraction kettle, and introducing supercritical CO 2 And 3g of methanol, the pressure is 30MPa, and the flow rate of the mixed fluid after gas mixing is 30L/h.
In the step (1), the process conditions of high-pressure homogenization are as follows: homogenizing under 100MPa for 3 times.
In the step (1), the complex enzyme comprises: 3g of alpha-amylase, 3g of neutral protease, 2g of cellulase and 1g of xylanase.
In the step (1), the enzymolysis process conditions are as follows: pH =5, temperature 60 ℃, enzymolysis time 45 minutes.
In the step (1), the spray drying process conditions are as follows: the air inlet temperature is 160 ℃, the air outlet temperature is 65 ℃, and the compressed air flow is 700mL/h.
In the step (2), the preparation method of the extract B is as follows:
(2-1) adding 10g of mixed powder into 55g of distilled water, heating and extracting for 2 hours at 90 ℃, and centrifuging to obtain an extracting solution I and a residue I;
(2-2) adding the residue I into 50g of absolute ethyl alcohol, heating and extracting for 4 hours at 50 ℃, and centrifuging to obtain an extracting solution II and a residue II;
(2-3) adding the residue II into 35g of petroleum ether, heating and extracting for 2 hours at 70 ℃, and centrifuging to obtain an extracting solution III;
and (2-4) finally, respectively spray-drying the extracting solution I, the extracting solution II and the extracting solution III, and then mixing to obtain the extract B.
In the step (3), the spray drying process conditions are as follows: the air inlet temperature is 165 ℃, the air outlet temperature is 60 ℃, and the compressed air flow is 600mL/h.
Example 2
A preparation method of an additive for electronic atomization liquid comprises the following specific steps:
(1) Uniformly dispersing 30g testa Tritici in 160g water, homogenizing under high pressure to obtain testa Tritici slurry, and supercritical CO 2 Extracting, performing enzymolysis with 1g of complex enzyme, inactivating enzyme, and centrifuging to obtain enzymePerforming spray drying on the hydrolysate to obtain an extract A;
(2) Respectively crushing 10g of rhizoma corydalis, 9g of subprostrate sophora and 3g of cortex lycii radicis to 300 meshes, mixing to obtain mixed powder, and sequentially extracting the mixed powder by using distilled water, absolute ethyl alcohol and petroleum ether to obtain an extract B;
(3) And then adding 15g of glucosyl stevioside and 15g of the extract A into 100g of water, stirring until the glucosyl stevioside and the extract A are completely dissolved, then adding 5g of the extract B, uniformly dispersing by ultrasonic waves, and carrying out spray drying to obtain the additive.
Wherein, in the step (1), supercritical CO is adopted 2 The specific method for extraction comprises the following steps: adding 25g of wheat bran slurry into an extraction kettle, and introducing supercritical CO 2 And 2g of methanol, the pressure is 35MPa, and the flow rate of the mixed fluid after gas mixing is 25L/h.
In the step (1), the process conditions of high-pressure homogenization are as follows: homogenizing under 120MPa for 2 times.
In the step (1), the complex enzyme comprises: 5g of alpha-amylase, 2g of neutral protease, 3g of cellulase and 0.8g of xylanase.
In the step (1), the enzymolysis process conditions are as follows: pH =6, temperature 50 ℃, enzymolysis time 55 minutes.
In the step (1), the spray drying process conditions are as follows: the air inlet temperature is 150 ℃, the air outlet temperature is 75 ℃, and the compressed air flow is 600mL/h.
In the step (2), the preparation method of the extract B is as follows:
(2-1) adding 10g of mixed powder into 65g of distilled water, heating and extracting for 3 hours at 80 ℃, and centrifuging to obtain an extracting solution I and a residue I;
(2-2) adding the residue I into 45g of absolute ethyl alcohol, heating and extracting for 3 hours at the temperature of 60 ℃, and centrifuging to obtain an extracting solution II and a residue II;
(2-3) adding the residue II into 45g of petroleum ether, heating and extracting for 3 hours at the temperature of 60 ℃, and centrifuging to obtain an extracting solution III;
and (2-4) finally, spray drying the extracting solution I, the extracting solution II and the extracting solution III respectively, and then combining to obtain the extract B.
In the step (3), the spray drying process conditions are as follows: the air inlet temperature is 155 ℃, the air outlet temperature is 70 ℃, and the compressed air flow rate is 500mL/h.
Example 3
A preparation method of an additive for electronic atomization liquid comprises the following specific steps:
(1) Uniformly dispersing 25g of testa Tritici in 170g of water, homogenizing under high pressure to obtain testa Tritici slurry, and supercritical CO 2 Extracting, performing enzymolysis with 0.9g of complex enzyme, inactivating enzyme, centrifuging to obtain enzymolysis solution, and spray drying to obtain extract A;
(2) Respectively crushing 11g of corydalis tuber, 8g of subprostrate sophora and 3.5g of cortex lycii radicis into 300 meshes, mixing to obtain mixed powder, and sequentially extracting the mixed powder by using distilled water, absolute ethyl alcohol and petroleum ether to obtain an extract B;
(3) And then adding 18g of glucosyl stevioside and 12g of the extract A into 100g of water, stirring until the glucosyl stevioside and the extract A are completely dissolved, then adding 6g of the extract B, uniformly dispersing by ultrasonic waves, and carrying out spray drying to obtain the additive.
Wherein, in the step (1), supercritical CO is adopted 2 The specific method for extraction comprises the following steps: adding 20g of wheat bran slurry into an extraction kettle, and introducing supercritical CO 2 And 2.5g of methanol, the pressure was 32MPa, and the flow rate of the mixed fluid after gas mixing was 28L/h.
In the step (1), the process conditions of high-pressure homogenization are as follows: homogenizing under 110MPa for 3 times.
In the step (1), the compound enzyme comprises: 4g of alpha-amylase, 2.5g of neutral protease, 2.5g of cellulase and 0.9g of xylanase.
In the step (1), the enzymolysis process conditions are as follows: pH =5, temperature 55 ℃, enzymolysis time 50 minutes.
In the step (1), the spray drying process conditions are as follows: the inlet air temperature is 155 ℃, the outlet air temperature is 70 ℃, and the compressed air flow is 650mL/h.
In the step (2), the preparation method of the extract B is as follows:
(2-1) adding 10g of mixed powder into 60g of distilled water, heating and extracting at 85 ℃ for 2.5 hours, and centrifuging to obtain an extracting solution I and a residue I;
(2-2) adding the residue I into 48g of absolute ethyl alcohol, heating and extracting for 3.5 hours at 55 ℃, and centrifuging to obtain an extracting solution II and a residue II;
(2-3) adding the residue II into 40g of petroleum ether, heating and extracting for 2.5 hours at 65 ℃, and centrifuging to obtain an extracting solution III;
and (2-4) finally, respectively spray-drying the extracting solution I, the extracting solution II and the extracting solution III, and then mixing to obtain the extract B.
In the step (3), the spray drying process conditions are as follows: the air inlet temperature is 160 ℃, the air outlet temperature is 65 ℃, and the compressed air flow is 550mL/h.
Comparative example 1
A preparation method of an additive for electronic atomization liquid comprises the following specific steps:
(1) Uniformly dispersing 20g of testa Tritici in 180g of water, homogenizing under high pressure to obtain testa Tritici slurry, and supercritical CO 2 Extracting, performing enzymolysis with 0.8g of complex enzyme, inactivating enzyme, centrifuging to obtain enzymolysis solution, and spray drying to obtain extract A;
(2) Respectively crushing 12g of rhizoma corydalis, 7g of subprostrate sophora and 4g of cortex lycii radicis to 200 meshes, mixing to obtain mixed powder, and sequentially extracting the mixed powder by using distilled water, absolute ethyl alcohol and petroleum ether to obtain an extract B;
(3) Then adding 10g of the extract A into 100g of water, stirring until the extract A is completely dissolved, then adding 7g of the extract B, uniformly dispersing by ultrasonic waves, and carrying out spray drying to obtain the additive.
Wherein, in the step (1), supercritical CO is adopted 2 The specific method for extraction comprises the following steps: adding 15g of wheat bran slurry into an extraction kettle, and introducing supercritical CO 2 And 3g of methanol, the pressure is 30MPa, and the flow rate of the mixed fluid after gas mixing is 30L/h.
In the step (1), the process conditions of high-pressure homogenization are as follows: homogenizing under 100MPa for 3 times.
In the step (1), the complex enzyme comprises: 3g of alpha-amylase, 3g of neutral protease, 2g of cellulase and 1g of xylanase.
In the step (1), the enzymolysis process conditions are as follows: pH =5, temperature 60 ℃, enzymatic time 45 minutes.
In the step (1), the spray drying process conditions are as follows: the air inlet temperature is 160 ℃, the air outlet temperature is 65 ℃, and the compressed air flow is 700mL/h.
In the step (2), the preparation method of the extract B is as follows:
(2-1) adding 10g of mixed powder into 55g of distilled water, heating and extracting for 2 hours at 90 ℃, and centrifuging to obtain an extracting solution I and a residue I;
(2-2) adding the residue I into 50g of absolute ethyl alcohol, heating and extracting for 4 hours at 50 ℃, and centrifuging to obtain an extracting solution II and a residue II;
(2-3) adding the residue II into 35g of petroleum ether, heating and extracting for 2 hours at 70 ℃, and centrifuging to obtain an extracting solution III;
and (2-4) finally, respectively spray-drying the extracting solution I, the extracting solution II and the extracting solution III, and then mixing to obtain the extract B.
In the step (3), the spray drying process conditions are as follows: the air inlet temperature is 165 ℃, the air outlet temperature is 60 ℃, and the compressed air flow is 600mL/h.
Comparative example 2
A preparation method of an additive for electronic atomization liquid comprises the following specific steps:
(1) Uniformly dispersing 20g of testa Tritici in 180g of water, homogenizing under high pressure to obtain testa Tritici slurry, and supercritical CO 2 Extracting, performing enzymolysis with 0.8g of complex enzyme, inactivating enzyme, centrifuging to obtain enzymolysis solution, and spray drying to obtain extract A;
(2) Pulverizing rhizoma corydalis 12g to 200 mesh to obtain powder, and sequentially extracting with distilled water, anhydrous ethanol, and petroleum ether to obtain extract B;
(3) And then adding 20g of glucosyl stevioside and 10g of the extract A into 100g of water, stirring until the glucosyl stevioside and the extract A are completely dissolved, then adding 7g of the extract B, uniformly dispersing by ultrasonic waves, and carrying out spray drying to obtain the additive.
Wherein, in the step (1), supercritical CO is adopted 2 The specific method for extraction comprises the following steps: adding 15g of wheat bran slurry into an extraction kettle, and introducing supercritical CO 2 And 3g of methanol, the pressure is 30MPa, and the flow rate of the mixed fluid after gas mixing is 30L/h.
In the step (1), the process conditions of high-pressure homogenization are as follows: homogenizing under 100MPa for 3 times.
In the step (1), the complex enzyme comprises: 3g of alpha-amylase, 3g of neutral protease, 2g of cellulase and 1g of xylanase.
In the step (1), the enzymolysis process conditions are as follows: pH =5, temperature 60 ℃, enzymolysis time 45 minutes.
In the step (1), the spray drying process conditions are as follows: the air inlet temperature is 160 ℃, the air outlet temperature is 65 ℃, and the compressed air flow is 700mL/h.
In the step (2), the preparation method of the extract B is as follows:
(2-1) adding 10g of powder into 55g of distilled water, heating and extracting for 2 hours at 90 ℃, and centrifuging to obtain an extracting solution I and a residue I;
(2-2) adding the residue I into 50g of absolute ethyl alcohol, heating and extracting for 4 hours at 50 ℃, and centrifuging to obtain an extracting solution II and a residue II;
(2-3) adding the residue II into 35g of petroleum ether, heating and extracting for 2 hours at 70 ℃, and centrifuging to obtain an extracting solution III;
and (2-4) finally, spray drying the extracting solution I, the extracting solution II and the extracting solution III respectively, and then combining to obtain the extract B.
In the step (3), the spray drying process conditions are as follows: the air inlet temperature is 165 ℃, the air outlet temperature is 60 ℃, and the compressed air flow is 600mL/h.
The additives obtained in examples 1 to 3 and comparative examples 1 and 2 are respectively used for preparing the electronic atomized liquid, and the specific method comprises the following steps: firstly, 55g of propylene glycol and 45g of glycerol are stirred and mixed uniformly, then 0.6g of additive and 1.2g of essence are added, and the mixture is stirred and mixed uniformly, so that the electronic atomization liquid is obtained.
10mL of each of the electron-atomized liquids was allowed to stand at 45 ℃ and 50 RH for 30 days, and the weight loss rate (%) = (weight after standing for 30 days-initial weight)/initial weight × 100% was calculated.
10mL of electronic atomized liquid is taken, atomized by an atomizer, and then the atomized liquid is collected for bacteriostasis effect investigation. The specific method comprises the following steps: selecting common pathogenic bacteria in oral cavity for culturing, specifically including Staphylococcus aureus, helicobacter pylori and Porphyromonas gingivalis, at 28 deg.C to obtain bacteria culture solution; sterilizing the beef extract culture medium added with the agar, adding the beef extract culture medium into an aseptic culture dish, solidifying, adding the bacterial culture solution into the culture dish, and uniformly coating; and then, punching a hole on the culture dish by using a sterilized stainless steel puncher with the outer diameter of 2mm, injecting atomized liquid into the hole by using a liquid transfer gun, respectively culturing at 28 ℃ for 24 hours, and measuring the diameter of the inhibition zone.
The results are shown in Table 1.
TABLE 1 results of investigating the properties of the electronic atomized liquid
Figure BDA0003373076970000111
As can be seen from Table 1, the electronic atomization liquids of examples 1 to 3 have small volatility and large bacteriostatic diameter, which indicates that the electronic atomization liquids have good bacteriostatic effect on common oral pathogenic bacteria.
Comparative example 1 glucose-based steviol glycoside, comparative example 2 omitting subprostrate sophora and cortex lycii radicis in the extract B, and the obtained electronic atomized liquid becomes more volatile and less antibacterial, which indicates that glucose-based steviol glycoside, the extract a and the extract B act synergistically, so that volatility is reduced and antibacterial property is improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (6)

1. A preparation method of an additive for electronic atomization liquid is characterized by comprising the following specific steps:
(1) Uniformly dispersing testa Tritici in water, homogenizing under high pressure to obtain testa Tritici slurry, and supercritical CO 2 Extracting, performing enzymolysis by using compound enzyme, inactivating enzyme, centrifuging to obtain enzymolysis liquid, and performing spray drying to obtain an extract A;
(2) Respectively crushing the corydalis tuber, the subprostrate sophora and the cortex lycii radicis to 200-300 meshes, mixing to obtain mixed powder, and sequentially extracting the mixed powder by using distilled water, absolute ethyl alcohol and petroleum ether to obtain an extract B;
(3) Adding glucosyl stevioside and the extract A obtained in the step (1) into water, stirring until the glucosyl stevioside and the extract A are completely dissolved, then adding the extract B obtained in the step (2), uniformly dispersing by ultrasonic waves, and performing spray drying to obtain the additive;
in the step (1), the mass ratio of wheat bran, water and complex enzyme is 20-30: 160-180: 0.8 to 1;
in the step (2), the preparation method of the extract B comprises the following steps in parts by weight:
(2-1) firstly adding 10 parts of mixed powder into 55-65 parts of distilled water, heating and extracting for 2-3 hours at the temperature of 80-90 ℃, and centrifuging to obtain an extracting solution I and residues I;
(2-2) adding the residue I into 45-50 parts of absolute ethyl alcohol, heating and extracting for 3-4 hours at 50-60 ℃, and centrifuging to obtain an extracting solution II and a residue II;
(2-3) adding the residue II into 35-45 parts of petroleum ether, heating and extracting for 2-3 hours at the temperature of 60-70 ℃, and centrifuging to obtain an extracting solution III;
(2-4) finally, spray-drying the extracting solution I, the extracting solution II and the extracting solution III respectively, and then combining to obtain the extract B;
in the step (3), the mass ratio of the glucosyl stevioside to the extract A to the water to the extract B is 15-20: 10-15: 100:5 to 7;
the compound enzyme comprises: 3-5 parts of alpha-amylase, 2-3 parts of neutral protease, 2-3 parts of cellulase and 0.8-1 part of xylanase.
2. The preparation method according to claim 1, wherein in the step (1), the process conditions of the high-pressure homogenization are as follows: homogenizing for 2-3 times under 100-120 MPa.
3. The preparation method according to claim 1, wherein in the step (1), the enzymolysis process conditions are as follows: pH = 5-6, temperature 50-60 deg.C, enzymolysis time 45-55 minutes.
4. An additive for an electronically atomized liquid obtained by the production method according to any one of claims 1 to 3.
5. Use of the additive of claim 4 in the preparation of an electronically atomised liquid.
6. An electronically atomized liquid comprising the additive of claim 4.
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