CN114097970A - Marine active peptide energy beverage and preparation method thereof - Google Patents
Marine active peptide energy beverage and preparation method thereof Download PDFInfo
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- CN114097970A CN114097970A CN202111520448.2A CN202111520448A CN114097970A CN 114097970 A CN114097970 A CN 114097970A CN 202111520448 A CN202111520448 A CN 202111520448A CN 114097970 A CN114097970 A CN 114097970A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention relates to the technical field of food health products, in particular to a marine active peptide energy beverage and a preparation method thereof. The material comprises the following raw materials in percentage by mass: 75-90 parts of drinking water, 5-8 parts of oyster peptide powder, 5-8 parts of sea cucumber peptide powder, 1-3 parts of taurine, 2-5 parts of inositol, 3-8 parts of nicotinamide and vitamin B60.02-0.05 part of vitamin B120.03-0.06 part, 1-3 parts of dietary fiber, 5-20 parts of sweetener, 0.1-0.3 part of acidity regulator, 0.01-0.04 part of edible essence, 0.02-0.04 part of preservative and 0.01-0.03 part of pigment. The invention adopts the raw materials rich in natural taurine, applies a scientific formula and a relatively simple production process to solve the problems of high sugar content, caffeine dependence, no human health and the like of the existing energy beverage, and the prepared product has the characteristics of diversified application scenes, suitability for students, athletes, old people and various groups of people, sour and sweet taste, elegant fragrance, attractive color and luster, high stability and long quality guarantee period.
Description
The technical field is as follows:
the invention relates to the technical field of food health products, in particular to a marine active peptide energy beverage and a preparation method thereof.
Background art:
the oysters are the first food listed as homology of medicine and food in China, and are rich in various amino acids, oyster polysaccharide, taurine, zinc and other nutritional ingredients. The micromolecular active peptide extracted from oyster meat keeps functional components in oyster to the greatest extent, has the effects of regulating and protecting organisms, can effectively relieve fatigue, and has good effects of improving immunity, strengthening liver, relieving alcoholism, reducing blood sugar, improving male serum testosterone level and the like. In addition, the natural taurine rich in the oyster peptide has bioactivity which is not possessed by the artificial chemical synthetic taurine, and the advantages are obvious.
The record of food materia medica: sea cucumber has the health-care function of removing deficiency and loss, and can tonify primordial qi and nourish internal organs. The sea cucumber contains more than 50 natural nutrient components and has extremely high nutritional value. The sea cucumber peptide is refined by adopting an international advanced enzymolysis technology, completely retains the specific nutrient components of the sea cucumber, converts macromolecular protein into micromolecular active peptide which is easier to absorb and has stronger function, and has more advantages compared with the traditional sea cucumber product.
Dietary fiber is a large group of carbohydrates that are not digested by the human body. It has been commonly referred to by the scientific community as nutrients, i.e., nutrients necessary for the human body, along with conventional proteins, fats, carbohydrates, vitamins, minerals, etc. Recent research proves that the dietary fiber has the functions of relieving constipation, promoting the growth of probiotics, protecting intestinal tracts, regulating blood sugar, preventing diabetes and the like.
With the progress and development of society, the mental stress is continuously increased after staying up and overtime, which causes people to have serious overdraft energy and physical strength, the overload operation of human body system, fatigue, weakness and weakness, and serious influence on work and life. In general, most people are selectively neglected in such a sub-health state without organic disease, and hidden troubles are buried in the process of finally developing into a disease state. Therefore, we should pay attention to the regulation of body functions, getting rid of sub-health status, and controlling the occurrence and development of diseases from the source.
At present, most of energy beverages in the market contain taurine, caffeine, guarana, white granulated sugar, vitamins, minerals and the like, and the energy beverages have a good refreshing effect on mature working crowds and crowds who are easy to fatigue in high-intensity work, but are not very suitable for student crowds who are not completely developed. In addition, the taurine in the formula is basically an artificial synthetic raw material and does not have the biological activity of natural taurine. Research shows that taurine is mainly metabolized by the kidney after being absorbed by a human body, the balance of the taurine content in the body is maintained, and the kidney burden is inevitably caused by excessive intake. Frequent drinking of such beverages in large quantities is liable to cause dependence and side effects are also relatively obvious. Meanwhile, the large amount of white granulated sugar is not suitable for people who lose weight, have blood sugar regulation dysfunction and type 2 diabetes. Therefore, the market urgently needs to provide a novel energy beverage which can fundamentally solve the above pain points for consumers, can meet the market trend and the requirements of the consumers, has natural and safe raw material components, and has the functions of resisting fatigue, resisting oxidation, supplementing energy, refreshing and calming the nerves and the like.
The invention content is as follows:
the invention aims to solve the technical problem that most energy beverages in the market contain taurine, caffeine, guarana, white granulated sugar, vitamins, minerals and the like, are not suitable for students who are not completely developed in body, weight-losing people, people with blood sugar regulation dysfunction and type 2 diabetes and are not suitable for the people, and meanwhile, the dependence is easy to generate and the side effect is obvious when the energy beverages are frequently drunk in a large amount.
In order to solve the problems, the invention provides a marine active peptide energy beverage and a preparation method thereof, the problems of high sugar content, caffeine dependence, no starting from the perspective of human health and the like of the existing energy beverage are solved by adopting a scientific formula and a relatively simple production process of raw materials rich in natural taurine, and the prepared product has diversified application scenes, is suitable for being used by various groups of students, athletes, old people and the like, and has the characteristics of sour and sweet taste, light and elegant fragrance, attractive color and luster, high stability and long shelf life.
In order to achieve the purpose, the invention realizes the marine active peptide energy beverage by the following technical scheme, which comprises the following raw materials in percentage by mass: 75-90 parts of drinking water, 5-8 parts of oyster peptide powder, 5-8 parts of sea cucumber peptide powder, 1-3 parts of taurine, 2-5 parts of inositol, 3-8 parts of nicotinamide and vitamin B60.02-0.05 part of vitamin B120.03-0.06 part, 1-3 parts of dietary fiber, 5-20 parts of sweetener, 0.1-0.3 part of acidity regulator, 0.01-0.04 part of edible essence, 0.02-0.04 part of preservative and 0.01-0.03 part of pigment.
The oyster peptide raw material added in the product is derived from triploid oysters of special natural sea areas, and the oysters from 10 months to next 4 months are selected, so that the oyster peptide feed is pollution-free, large in size, high in meat quality and rich in nutrition. The solubility reaches more than 99 percent; the stability is good, the coexistence stability with other components is good, and the storage stability is good; low viscosity; good processability; low osmotic pressure and low irritability; has no fishy smell and good palatability. Through analysis, the composition of the essential amino acids of the oyster peptides reaches 55.7g/100g, which is higher than that of milk protein (50.0g/100g) and egg protein (49.7g/100 g). The polysaccharide content is more than or equal to 15.0 percent, and the natural taurine content is more than or equal to 1500mg/100 g.
Stichopus japonicus peptide raw material added in the product is from Arctic Stichopus japonicus of Canada, and research shows that the body wall of the Arctic Stichopus japonicus contains abundant mucopolysaccharide, and is rich in Stichopus japonicus saponin, vitamins and microelements. The sea cucumber peptide has high purity, the protein content is more than or equal to 90 percent, and the sea cucumber peptide has good thermal stability; good processability; low osmotic pressure and low irritability; has no fishy smell and good palatability. The sea cucumber peptide has molecular weight lower than 10000Da, contains 18 kinds of amino acids, and has polysaccharide content not less than 5%.
Taurine has antiinflammatory, antipyretic, analgesic, tranquilizing, blood pressure lowering, blood sugar lowering, arrhythmia resisting, antibacterial, platelet aggregation resisting, immunity enhancing, gallbladder function promoting, liver strengthening, toxic materials clearing away, and vascular tension regulating effects
Inositol is a kind of biological active element, is involved in metabolism, and has immunity, disease preventing and treating effects
Pellagra is caused by lack of nicotinamide in the body, so the product can prevent pellagra. Has effects in protein and sugar metabolism, and can improve nutrition of human and animal.
General symptoms of the deficiency of vitamin B6 include inappetence, low food utilization rate, weightlessness, vomiting, diarrhea and other diseases. Severe deficiency can be manifested by acne, anemia, arthritis, childhood spasm, depression, headache, hair loss, easy inflammation, learning disability, asthenia, etc.
Vitamin B12 is the only vitamin that needs the help of intestinal secretions (endogenous factors) to be absorbed, and is involved in the production of bone marrow red blood cells, prevention of pernicious anemia, and prevention of damage to the cerebral nerves.
The eating of the dietary fiber is less, so that the disease rate of the modern civilization diseases such as obesity, diabetes, hyperlipidemia and the like and the disease related to the insufficient dietary fiber such as intestinal cancer, constipation, intestinal polyp and the like are gradually increased.
Modern biological enzymolysis technology: the target active peptide is obtained by a specific biological enzyme preparation enzymolysis technology and has high purity. The combination of the immunity strengthening function of the sea cucumber and the functions of replenishing essence and tonifying qi of the oyster is beneficial to the rapid recovery of primordial qi of consumers, so that caffeine is replaced, and the health-care tea is safer and healthier.
Contains two marine active peptide components of oyster peptide and sea cucumber peptide, wherein the content of the oyster peptide is more than or equal to 1000mg/L, the content of the sea cucumber peptide is more than or equal to 600mg/L, and the content of natural taurine in the oyster peptide is more than or equal to 1500mg/100 g.
Further, the dietary fiber is composed of at least one of polydextrose, xylooligosaccharide, resistant dextrin, isomaltooligosaccharide pulp and carboxymethyl methyl cellulose.
Further, the sweetener is at least one of xylitol, maltitol, mogroside, stevioside, monopotassium glycyrrhizinate, mannitol and erythritol.
A preparation method of the marine active peptide energy beverage comprises the steps of mixing, dissolving, filtering, filling, sterilizing and cooling.
Further, the ingredients comprise the preparation of a base stock and the preparation of an acidity regulator.
The base material is prepared by adding oyster peptide, sea cucumber peptide, taurine and softened water with the temperature of 60-90 ℃, fully stirring and dissolving, then sequentially adding dietary fiber, inositol, nicotinamide, vitamin B6, B12 and a sweetening agent, and uniformly mixing;
the preparation of the acidity regulator is to fully and uniformly stir citric acid and sodium citrate in sequence.
Further, the mixing is to slowly add the acid material into the base material under stirring at normal temperature, then slowly add the edible essence, and perform constant volume after uniform mixing to obtain mixed material liquid.
Further, sterilization is to keep the filled beverage at 85-95 ℃ for 15-20 min.
Further, the preparation method of the oyster peptide powder comprises the following steps:
(1) removing shell of Concha Ostreae, taking edible part, quick freezing with liquid nitrogen, homogenizing, and inactivating enzyme to obtain Concha Ostreae powder;
(2) adding an ammonium bicarbonate solution into the oyster powder, centrifuging, and collecting supernatant to obtain a peptide crude extract;
(3) and adding dithiothreitol into the crude peptide extract, performing water bath shaking reaction, cooling to room temperature, adding iodoacetamide solution, performing light-resistant reaction at room temperature, performing solid-phase extraction desalting treatment, and performing freeze-drying treatment on the desalted solution to obtain the oyster peptide raw material.
Further, the preparation method of the sea cucumber peptide powder comprises the following steps:
(1) removing viscera and muscle layer of sea cucumber, cleaning, and pulverizing;
(2) adding 40-80% alcohol water solution by volume percent at room temperature for leaching for 3-7 d;
(3) centrifuging, and repeatedly washing the obtained precipitate with clear water; adding 0.05-0.3M NaOH aqueous solution to soak for 10-48 h;
(4) centrifuging, and repeatedly washing the obtained precipitate with clear water until the pH value is neutral;
(5) adding deionized water into the precipitate, adding compound flavor protease under stirring, and performing enzymolysis for 2-6h at 45-55 deg.C and pH of 7-8;
(6) inactivating enzyme at 90-100 deg.C for 4-10 min; centrifuging the enzymolysis liquid, and separating supernatant by using an ultrafiltration membrane;
(7) collecting the components with the molecular weight less than or equal to 6KDa for decolorization, desalting, vacuum concentrating, and freeze drying to obtain the sea cucumber collagen active peptide powder with the molecular weight range of 200-1800 Da.
The invention has the beneficial effects that:
(1) oyster peptide and sea cucumber peptide are extracted from the raw materials in a pure natural way, so that the fatigue state of a human body is effectively relieved, the digestion and absorption of nutrient substances and the energy utilization are improved, and the sub-health states of listlessness and the like are relieved;
(2) the ocean active peptide energy beverage starts from the health preserving perspective, adjusts the human body to the optimal state, refreshes and is not excited, is suitable for supplementing the mental activity after the human body feels fatigue, resists the powerless feeling, has diversified application scenes compared with energy beverages with higher sugar content such as caffeine, artificial taurine and the like, and can be widely applied to various groups of people, such as students, old people, weight-losing and type 2 diabetes mellitus people and the like;
(3) the oyster peptide and the oyster peptide have the effects of enhancing the immune function of organisms, resisting tumors, resisting viruses and the like, have important values on preventing diseases and delaying aging, can regulate the body health after being drunk for a long time, and have no side effect.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a marine active peptide energy beverage is prepared from the following raw materials: 85 parts of drinking water, 5 parts of oyster peptide powder, 5 parts of sea cucumber peptide powder, 2 parts of taurine, 3 parts of inositol, 3 parts of nicotinamide, 0.03 part of B6, 0.04 part of B12, 2 parts of dietary fiber, 19 parts of sweetening agent, 0.1 part of acidity regulator, 0.02 part of edible essence, 0.03 part of preservative and 0.02 part of cochineal.
The preparation method of the marine active peptide energy beverage comprises the following steps:
the invention relates to a method for preparing a marine active peptide energy beverage, which comprises the following steps:
(1) putting 85 parts of drinking water, 5 parts of oyster peptide powder, 5 parts of sea cucumber peptide powder, 2 parts of taurine, 3 parts of inositol, 3 parts of nicotinamide, 0.03 part of B6, 0.04 part of B12, 2 parts of dietary fiber, 19 parts of sweetening agent, 0.02 part of edible essence, 0.03 part of preservative and 0.02 part of cochineal carmine into a stirring tank in sequence, stirring for 3-5 minutes, adding 0.1 part of acidity regulator at normal temperature, and uniformly mixing;
(2) filtering the mixture by a filter with the filtering grade of 10-50 mu m, wherein the filtered feed liquid is transparent, tasteless and free of visible matters;
(3) filling the clear liquid, sterilizing the filling product, and sealing the cap.
Example 2:
a marine active peptide energy beverage is prepared from the following raw materials: 90 parts of drinking water, 6 parts of oyster peptide powder, 6 parts of sea cucumber peptide powder, 3 parts of taurine, 2 parts of inositol, 4 parts of nicotinamide, 0.04 part of B6, 0.05 part of B12, 3 parts of dietary fiber, 20 parts of sweetening agent, 0.2 part of acidity regulator, 0.03 part of edible essence, 0.04 part of preservative and 0.03 part of lemon yellow.
The preparation method of the marine active peptide energy beverage comprises the following steps:
the invention relates to a method for preparing a marine active peptide energy beverage, which comprises the following steps:
(4) putting water, oyster peptide powder 6 parts, sea cucumber peptide powder 6 parts, taurine 3 parts, inositol 2 parts, nicotinamide 4 parts, B6 0.04 parts, B12 0.05 parts, dietary fiber 3 parts, sweetening agent 20 parts, acidity regulator 0.2 parts, edible essence 0.03 parts, preservative 0.04 parts and lemon yellow 0.03 parts into a stirring tank in sequence, stirring for 3-5 minutes, adding acidity regulator 0.2 parts at normal temperature, and mixing uniformly;
(5) filtering the mixture by a filter with the filtering grade of 10-50 mu m, wherein the filtered feed liquid is transparent, tasteless and free of visible matters;
(6) filling the clear liquid, sterilizing the filling product, and sealing the cap.
Example 3:
a marine active peptide energy beverage is prepared from the following raw materials: 90 parts of drinking water, 7 parts of oyster peptide powder, 7 parts of sea cucumber peptide powder, 3 parts of taurine, 4 parts of inositol, 6 parts of nicotinamide, 0.05 part of B6, 0.06 part of B12, 3 parts of dietary fiber, 20 parts of sweetening agent, 0.3 part of acidity regulator, 0.04 part of edible essence, 0.04 part of preservative and 0.03 part of cochineal.
The preparation method of the marine active peptide energy beverage comprises the following steps:
the invention relates to a method for preparing a marine active peptide energy beverage, which comprises the following steps:
(4) sequentially putting 90 parts of drinking water, 7 parts of oyster peptide powder, 7 parts of sea cucumber peptide powder, 3 parts of taurine, 4 parts of inositol, 6 parts of nicotinamide, 0.05 part of B6, 0.06 part of B12, 3 parts of dietary fiber, 20 parts of sweetening agent, 0.3 part of acidity regulator, 0.04 part of edible essence, 0.04 part of preservative and 0.03 part of cochineal in a stirring tank, stirring for 3-5 minutes, adding 0.3 part of acidity regulator at normal temperature, and uniformly mixing;
(5) filtering the mixture by a filter with the filtering grade of 10-50 mu m, wherein the filtered feed liquid is transparent, tasteless and free of visible matters;
(6) filling the clear liquid, sterilizing the filling product, and sealing the cap.
Evaluation of example 2:
first, effect evaluation (score of 5)
30 volunteers are selected to drink the marine active peptide energy beverage of the example 2, and the taste, the effect and the like of the volunteers are tested by 6-month tracking test, and the detection results are shown in the following table:
the research and long-time experimental results show that the marine active peptide energy beverage has obvious effects on relieving physical fatigue, enhancing immunity and the like.
Secondly, animal experiment verification:
1 materials and methods
1.1 sample: the marine active peptide energy drink (concentrated solid powder) is inspected by Qingdao ocean food institute, the inspected sample is milk white solid, the ultra pure water is prepared into corresponding concentration before use, and the pure content of the sample is approved: 260 g/bag, the recommended daily intake of each person of the marine active peptide energy beverage is 280ml/60kg & BW, and the storage conditions are as follows: storing at 4 ℃. Solvent: ultrapure water.
1.2 comparative samples: the red bull vitamin flavored beverage is produced by Tencel medicine health care Limited company (Thailand), a sample is yellow liquid, and the daily intake of each person is recommended to be 250 ml. Sample batch number: 20210616, shelf life: and (4) 18 months. 12500ml of vitamin-flavored beverage is rotary evaporated at low temperature (50 ℃) into 2000ml for standby application before use.
1.2 Experimental animals: 336 SPF grade ICR mice; male; initial weight range: 18-22 g; offered by sbefu (beijing) bio ltd; animal certification number: SCXK (Jing) 2019-; animals were kept in a barrier environment and observed for testing 3 days later. License number for experimental animals: SYXK (Kyoto) 2017-. The animal feed is provided by Australian cooperative feed Co.Ltd of Beijing family, and has the feed qualification number SCXK (Beijing) 2019-.
1.3 dose selection and subject administration: setting a negative control group (ultrapure water) and three dose groups of the marine active peptide energy drink: low dose group (14.0 ml/kg-BW), medium dose group (46.7 ml/kg-BW) and high dose group (140.0 ml/kg-BW) (corresponding to 3 times, 10 times and 30 times of the intended daily intake of human respectively), three dose groups of red bull vitamin flavored beverage: low dose (12.5 ml/kg. BW), medium dose (41.7 ml/kg. BW), high dose group (125 ml/kg. BW) (corresponding to 3 times, 10 times and 30 times daily intake, respectively, in humans). Test and control sample administration: gavage is carried out orally, and the concentration is 0.2ml/10g & BW.
1.4 main instruments and reagents: swimming box (50cm × 50cm × 40cm), SPN3001F electronic balance, lead sheet, stopwatch, Hitachi 7020 full-automatic biochemical analyzer, UV2100 ultraviolet spectrophotometer, centrifuge, and constant temperature water bath box; a Lactate analyzer (Lactate SCOUT), Lactate test paper (Lactate SCOUT Sensors Big Pack 72+, lot number: 1909827254), a liver glycogen/muscle glycogen assay kit (BB-4723, Shanghai Bibo Biotech Co., Ltd.), and a urea nitrogen kit (MAKER Biochemical kit).
1.5 test methods: grouping: the 336 animals were divided into 4 subgroups of 84 animals each. Each subgroup of animals was randomly divided into 7 groups by weight, namely a negative control group, a marine active peptide energy drink low dose group, a medium dose group, a high dose group, and a red bull vitamin flavored drink low dose group, a medium dose group, and a high dose group, each group containing 12 animals. Weighing a certain amount of concentrated solid powder of the marine active peptide energy drink, and dissolving the concentrated solid powder with ultrapure water to prepare a solution with a required concentration. 12500ml of red ox vitamin-flavored beverage is rotary evaporated at low temperature (50 deg.C) to 2000ml to obtain
The gavage solution with the concentration required by the high-dosage group is diluted by ultrapure water to prepare the solution with the corresponding concentration. The animals in each group are respectively fed with marine active peptide energy drink and red bull vitamin flavor drink with different dosages for intragastric administration, the negative control group is fed with ultrapure water, and the intragastric administration capacity of the mice is 0.2ml/10g & BW. The stomach was perfused 1 time daily for the same time. The weight is weighed every week to adjust the gavage amount, and the gavage is continuously carried out for 30 days. Each group of mice was fed with normal diet and had free access to food and water. And after 30 days, the physical fatigue relieving function test is carried out on each subgroup of mice.
1.5.1 weight swimming experiment: in the 1 st subgroup, 12 mice in each dose group are loaded with a test sample 30min later, then the tail root of the rat is loaded with 5% weight of lead skin, and then the mice are placed in a swimming box to swim (the water depth is more than or equal to 30cm, and the water temperature is 25 +/-1.0 ℃), and the time from the beginning of swimming to death of the mice is recorded as the weight bearing swimming time of the mice.
1.5.2 serum Urea Nitrogen determination: in the subgroup 2, 12 mice in each dose group are subjected to test substance administration for 30min in the last time, and then swim in water at the temperature of 30 ℃ for 90min without load, blood is collected and serum is separated after rest for 60min, serum urea nitrogen is measured by a Hitachi 7020 full-automatic biochemical analyzer, and a MAKER biochemical kit is adopted as a reagent.
1.5.3 liver glycogen assay: and in the 3 rd subgroup, after 12 mice in each dose group are subjected to the test object for 30min in the last time, the animals are killed, the liver is taken, rinsed by physiological saline and then is sucked dry by filter paper, 0.1-0.2g of the liver is accurately weighed, and the detection is carried out according to the kit instruction.
1.5.4 lactic acid determination: and 4, after 12 mice in each dose group of the 4 th subgroup are subjected to last intragastric administration for 30min, 1-2mm is cut, a cotton ball is used for wiping first blood drop, trace tail blood is collected, and the blood lactic acid content of the mice in the resting state is measured by a lactic acid measuring instrument. Then the mice swim in water with the temperature of 30 ℃ for 10min without bearing a load, and the blood lactic acid values of the mice immediately after exercise and after rest are detected by a lactic acid tester at 0min and 20min after swimming respectively.
1.6 statistics of test data: comparing differences between animal body weight, urea nitrogen, liver glycogen, blood lactic acid (before swimming, 0min after swimming, 20min after swimming) and negative control group by using an analysis of variance method, wherein p <0.05 is statistical; the difference between each experimental group and the negative control group is compared by analysis of variance after reciprocal transformation and COS transformation due to the uneven variance of the area under the load swimming time and the blood lactic acid curve, and p <0.05 is statistically significant.
1.7 judging the result: the weight-bearing swimming time: if the weight-bearing swimming time of the test sample group is obviously longer than that of the control group, and the difference is significant, the test result can be judged to be positive; urea nitrogen: if the serum urea of the test sample group is lower than that of the negative control group and the difference is significant, the test result can be judged to be positive; hepatic glycogen: if the content of hepatic glycogen of the test sample group is obviously higher than that of the negative control group, and the difference is significant, the experimental result can be judged to be positive; blood lactic acid: the area under the blood lactic acid curve of the three time points is taken as a judgment standard. The area of any test group is smaller than that of the negative control group, and the difference is significant, so that the test result can be judged to be positive. The test sample has positive load swimming test result and positive blood lactate, serum urea and glycogen blood glucose, and can be judged to have the function of relieving physical fatigue.
2, results:
2.1 weight gain in animals
After the marine active peptide energy drink is administered for 30 days, compared with the negative control group, the terminal weight of the animals in the low dose, medium dose and high dose groups in the second subgroup is obviously lower than that of the negative control group, the difference is significant (p <0.05 or p <0.01), the weight increase of the animals in the medium dose and high dose groups is obviously reduced, and the difference is significant (p < 0.05); the terminal weight and weight gain of the animals in the low-dose group in the fourth subgroup are obviously reduced, the difference is significant (p is less than 0.05), and the weight of mice in each dose group in the rest subgroups has no obvious difference compared with a negative control group and a negative control group (see table 1).
The vitamin-flavored drink is given to the red cattle for 30 days, compared with the negative control group, the terminal weight and the weight gain of the animals in the low dose, the medium dose and the high dose groups in the second subgroup are obviously lower than those in the negative control group, and the difference is significant (p is less than 0.01); the end body weight and weight gain of the fourth subgroup of high dose animals were significantly reduced, with a significant difference (p < 0.05). The weights of mice in each dose group of the other subgroups have no obvious difference compared with the negative control group and the negative control group (see table 1).
Although the animals in each dose group administered with the marine active peptide energy drink and the red bull vitamin flavored drink in the second subgroup had significantly reduced terminal weight and weight gain, the animals in the negative control group in the second subgroup had significantly increased terminal weight and weight gain compared to those in the negative control groups in the other three subgroups. Thus, the marine active peptide energy drink and red bull vitamin flavored drink may not be the primary cause of reduced terminal body weight and weight gain in the second subgroup of animals. Furthermore, the end body weight and weight gain of individual animals of the fourth subgroup were reduced and there was no dose-response relationship. In summary, administration of the marine active peptide energy drink and the red bull vitamin flavored drink to mice for 30 days may not have a significant effect on the body weight of the mice.
2.2 influence of marine active peptide energy drink on mouse weight bearing swimming time: when the marine active peptide energy drink is given for 30 days, compared with a negative control group, the weight-bearing swimming time of mice in a low-dose group is obviously prolonged, and the difference is significant (p is less than 0.01); when the vitamin-flavored drink is given to the red cattle for 30 days, the weight swimming time of the mice in the medium-dose group is obviously prolonged compared with that of the negative control group, and the difference is significant (p <0.01) (see table 2).
TABLE 1 Effect of Marine active peptide energy drinks on weight gain in mice
Note: *. shows significant difference compared with negative control group (p < 0.05);
indicates that the difference was significant compared to the negative control group (p < 0.01).
A. Marine active peptide energy beverage
B. Red bull vitamin flavored beverage
TABLE 2 Effect of Marine active peptide energy drinks on weight bearing swimming time of mice
Note: indicates that the difference is significant compared with the negative control group (p <0.01)
2.3 Effect of Marine active peptide energy drink on serum Urea Nitrogen: the marine active peptide energy drink is given for 30 days, compared with a negative control group, the mice in a high-dose group swim for 90min without load, and after resting for 60min, the serum urea nitrogen level is obviously reduced (p is less than 0.01); the serum urea nitrogen levels in the high dose group mice were significantly reduced (p <0.01) compared to the negative control group (see table 3) when the vitamin flavored red cattle beverages were administered for 30 days.
TABLE 3 Effect of Marine active peptide energy drinks on serum Urea Nitrogen and liver glycogen in mice
Note:**indicates that the difference is significant compared with the negative control group (p)<0.01)。
aThe number of animals is 11 (one animal is drowned during swimming)
2.4 Effect of Marine active peptide energy drinks on liver glycogen: when the marine active peptide energy drink is given for 30 days, compared with a negative control group, the liver glycogen level of mice in each dose group has no obvious change; when vitamin-flavored drink of red cattle is given for 30 days, compared with a negative control group, the glycogen level of mice in a medium-dose group and a high-dose group is obviously reduced, and the difference is significant (p <0.01) (see table 3).
2.5 Effect of Marine active peptide energy drink on blood lactic acid: the blood lactic acid level of the mice in the dose group in the marine active peptide energy beverage after swimming is obviously reduced compared with the blood lactic acid level of the mice in the negative control group, and the difference is significant (p is less than 0.05); compared with a negative control group, the area difference between the blood lactic acid curves of the dose group in the marine active peptide energy beverage at three time points of 0min before swimming, 0min after swimming and 20min after swimming is obviously reduced, and the difference has significance (p is less than 0.05). The blood lactic acid level of the mice in the high-dose group of the red bull vitamin flavored beverage before swimming, the blood lactic acid level of the mice in the medium-dose group and the high-dose group after swimming for 0min and the blood lactic acid level of the mice in the high-dose group after swimming for 20min are all obviously higher than the blood lactic acid level of the mice in the negative control group at corresponding time points, and the difference is significant (p <0.05 and/or p < 0.01); compared with the areas under the blood lactic acid curves of the mice in the negative control group at three time points of 0min before swimming, 0min after swimming and 20min after swimming, the areas under the blood lactic acid curves of the mice in the high-dose red bull vitamin flavored beverage at three time points of 0min before swimming, 0min after swimming and 20min after swimming are obviously increased, and the difference has significance (p <0.01) (see table 4) the influence (mmol/L) of the marine active peptide energy drink in table 4 on the blood lactic acid of the mice
Note:*indicates that the difference is significant compared with the negative control group (p)<0.05)；
**Indicates that the difference is significant compared with the negative control group (p)<0.01)。
aAfter logarithmic transformation is adopted, the data are normally distributed, all the components are uniform, and single-factor variance analysis is adopted;
bafter logarithmic transformation, the data are arbitrarily non-normally distributed, using rank sum analysis.
3 small knot
Animal experiment research shows that: after the mice are fed with 14.0, 46.7 and 140.0ml/kg & BW marine active peptide energy drinks by gastric lavage for 30 days continuously, the weight-bearing swimming time of the mice is obviously prolonged; the serum urea nitrogen level is obviously reduced; the area under the blood lactic acid curve is obviously reduced at three time points of 0min before swimming, 0min after swimming and 20min after swimming; but has no obvious influence on the liver glycogen level of the mouse in a resting state. According to the judgment standard of physical fatigue relieving of health food inspection and evaluation technical specification (2003 edition), the ocean active peptide energy drink can be considered to have the function of relieving physical fatigue.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any person skilled in the art can change or modify the above technical content into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (9)
1. A marine active peptide energy beverage is characterized by comprising the following raw materials in percentage by mass: 75-90 parts of drinking water, 5-8 parts of oyster peptide powder, 5-8 parts of sea cucumber peptide powder, 1-3 parts of taurine, 2-5 parts of inositol, 3-8 parts of nicotinamide and vitamin B60.02-0.05 part of vitamin B120.03-0.06 part, 1-3 parts of dietary fiber, 5-20 parts of sweetener, 0.1-0.3 part of acidity regulator, 0.01-0.04 part of edible essence, 0.02-0.04 part of preservative and 0.01-0.03 part of pigment.
2. The marine-active peptide energy beverage of claim 1, wherein: the dietary fiber is composed of at least one of polydextrose, xylooligosaccharide, resistant dextrin, isomaltooligosaccharide pulp and carboxymethyl methyl cellulose.
3. The marine-active peptide energy beverage of claim 1, wherein: the sweetener is at least one of xylitol, maltitol, mogroside, stevioside, monopotassium glycyrrhizinate, mannitol and erythritol.
4. The marine-active peptide energy beverage of claim 1, wherein: the preparation method of the oyster peptide powder comprises the following steps:
(1) removing shell of Concha Ostreae, taking edible part, quick freezing with liquid nitrogen, homogenizing, and inactivating enzyme to obtain Concha Ostreae powder;
(2) adding an ammonium bicarbonate solution into the oyster powder, centrifuging, and collecting supernatant to obtain a peptide crude extract;
(3) and adding dithiothreitol into the crude peptide extract, performing water bath shaking reaction, cooling to room temperature, adding iodoacetamide solution, performing light-resistant reaction at room temperature, performing solid-phase extraction desalting treatment, and performing freeze-drying treatment on the desalted solution to obtain the oyster peptide raw material.
5. The marine-active peptide energy beverage of claim 1, wherein: the preparation method of the sea cucumber peptide powder comprises the following steps:
(1) removing viscera and muscle layer of sea cucumber, cleaning, and pulverizing;
(2) adding 40-80% alcohol water solution by volume percent at room temperature for leaching for 3-7 d;
(3) centrifuging, and repeatedly washing the obtained precipitate with clear water; adding 0.05-0.3M NaOH aqueous solution to soak for 10-48 h;
(4) centrifuging, and repeatedly washing the obtained precipitate with clear water until the pH value is neutral;
(5) adding deionized water into the precipitate, adding compound flavor protease under stirring, and performing enzymolysis for 2-6h at 45-55 deg.C and pH of 7-8;
(6) inactivating enzyme at 90-100 deg.C for 4-10 min; centrifuging the enzymolysis liquid, and separating supernatant by using an ultrafiltration membrane;
(7) collecting the components with the molecular weight less than or equal to 6KDa for decolorization, desalting, vacuum concentrating, and freeze drying to obtain the sea cucumber collagen active peptide powder with the molecular weight range of 200-1800 Da.
6. A process for preparing a marine active peptide energy beverage as claimed in claim 1, characterised in that it comprises the steps of dosing, mixing, dissolving, filtering, filling, sterilising and cooling.
7. The method of claim 6, wherein: the ingredients comprise the preparation of a base material and the preparation of an acidity regulator: the base material is prepared by adding oyster peptide, sea cucumber peptide, taurine and softened water with the temperature of 60-90 ℃, fully stirring and dissolving, then sequentially adding dietary fiber, inositol, nicotinamide, vitamin B6, B12 and a sweetening agent, and uniformly mixing;
the preparation of the acidity regulator is to fully and uniformly stir citric acid and sodium citrate in sequence.
8. The method of claim 6, wherein: the mixing is to slowly add the acid material into the base material under stirring at normal temperature, then slowly add the edible essence, and after uniform mixing, fix the volume to obtain the mixed material liquid.
9. The method of claim 6, wherein: and sterilizing, namely keeping the filled beverage at 85-95 ℃ for 15-20 min.
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