CN108185339A - The beautiful enzyme composition of promise and its preparation - Google Patents
The beautiful enzyme composition of promise and its preparation Download PDFInfo
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- CN108185339A CN108185339A CN201711443906.0A CN201711443906A CN108185339A CN 108185339 A CN108185339 A CN 108185339A CN 201711443906 A CN201711443906 A CN 201711443906A CN 108185339 A CN108185339 A CN 108185339A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 68
- 239000000284 extract Substances 0.000 claims abstract description 60
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims abstract description 42
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims abstract description 42
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- 240000000851 Vaccinium corymbosum Species 0.000 claims abstract description 41
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- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 8
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/01—Instant products; Powders; Flakes; Granules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to technical field of health care food, the more particularly to beautiful enzyme composition of promise and its preparation.The beautiful enzyme composition of the promise is made of the beautiful ferment juice of promise, blueberry juice, Ginger P.E, red date extract and dried tremella extract.After the beautiful ferment juice of promise, blueberry juice, Ginger P.E, red date extract and dried tremella extract are used cooperatively by the present invention, significant synergistic function can be generated in terms of anti-aging, strengthen immunity；The beautiful enzyme composition of promise provided by the invention and its preparation contain abundant nutritional ingredient, and mellow, the intrinsic acid smell of no beautiful ferment of promise, mouthfeel is greatly improved, easy for consumers to accept.
Description
Technical field
The present invention relates to technical field of health care food, the more particularly to beautiful enzyme composition of promise and its preparation.
Background technology
Nuo Li (Morinda citrifolia Linn.) is Rubiaceae Morinda plant also known as Morinda, and the torrid zone is often
Green pulpous state multiple fruit, perennial broadleaf dungarunga rise in Pacific Ocean south island, through investigating, there is wild Nuo Lifa in Hainan Island
It is existing.Noni fruit contains minerals needed by human and vitamin and a variety of sugar and amino acid.Noni fruit contains quite high
Vegetable soda and plant polyphenol, it is identifiable at present to have 23 kinds.Additionally contain 5 kinds of vitamins and 3 kinds of human bodies
Required trace element.Noni fruit can be squeezed into fruit juice or hair because with health role and containing there are many nutriment
Fermented juice is extracted after ferment and is used for health care.At present, the relevant product of Noni fruit has：The beautiful ferment of promise, Morinda Citrifolia juice etc., but existing
In technique, the beautiful distinctive acid smell of ferment juice of promise after fermentation is difficult to be received by consumer, product can not large-scale popularization, industry
It can not run business big and strong, therefore, the beautiful enzyme preparation of promise for developing no acid smell is very necessary.
In Blueberry other than conventional sugar, acid and Vc, rich in VE、VA、VB, SOD, ursin, protein, anthocyanin,
The mineral elements such as dietary fibre and abundant K, Fe, Zn, Ca, have prevent cranial nerve aging, protection eyesight, cardiac stimulant, anticancer,
Softening blood vessel, enhancing human organism such as are immunized at the functions.Some are similar for the nutritional ingredient and effect and Noni fruit of blueberry, and fruit is in indigo plant
Color, color and luster are beautiful, and pulp is fine and smooth, seed is minimum, blueberry inspissated juice sweet and sour palatability, and with fragrant refreshing pleasant fragrance, mouthfeel pole
It is good.
Ginger (Zingiber officinale Roscoe), alias have race, hundred peppery clouds, hook dress to refer to, because ground is pungent, hot and cold
Boy, fresh ginger, honey toast ginger.Rhizome (rhizoma zingiberis), cork (ginger peel), the leaf (ginger leaves) of ginger can be used as medicine.Ginger is in traditional Chinese medicine and pharmacy
In there is diverging, preventing or arresting vomiting, cough-relieving and other effects.
Jujube also known as jujube, belong to Angiospermae, Dicotyledoneae, Rhamnales, Rhamnaceae, zizyphus plant.Its
Vitamin content is very high, there is the good reputation of " natural complex ball ", has nourishment for vitality, the effect of enriching blood.The herbal medicine book of China
Nationality《This warp》Described in arrive, jujube warm-natured, returns spleen stomach sweet in flavor, have tonifying middle-Jiao and Qi, nourishing blood and tranquilization, mitigate pharmacological property function；And
Modern pharmacology then finds, jujube contain protein, carbohydrate, organic acid, vitamin A, vitamin C, lot of trace calcium and
The abundant nutrition such as amino acid.
White fungus is also referred to as tremella, tremella, white fungus etc., belongs to Mycophyta Tremellaceae Tremella, is that a Basidiomycota is true
The fructification of bacterium white fungus has the laudatory title of " hat in bacterium ".The effect of white fungus is sweet in flavor, light, mild-natured, nontoxic, existing tonifying spleen appetizing, again
There are QI invigorating gut purge, nourishing Yin and moistening lung.Body immunity can be enhanced and enhance tolerance of the tumor patient to Radiotherapy chemotherapy
Power.White fungus has the function of enriching yin rich in natural plant colloid, additional its, is the good moisturizing food that can be taken for a long time.
At present, Jiang Nuoli, blueberry, ginger, jujube, white fungus be yet there are no with the use of the report as health product.
Invention content
In view of this, the present invention provides the beautiful enzyme composition of promise and its preparations.The beautiful enzyme composition of the promise is by the beautiful ferment of promise
After plain juice, blueberry juice, Ginger P.E, red date extract and dried tremella extract are used cooperatively, in anti-aging, strengthen immunity side
Face can generate significant synergistic function.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides a kind of beautiful enzyme compositions of promise, are carried by the beautiful ferment juice of promise, blueberry juice, Ginger P.E, jujube
Object and dried tremella extract is taken to form.
The present invention can not only improve mouthfeel using blueberry juice as flavoring agent, and the effect of can strengthen promise beautiful ferment product.
The combination of ginger, jujube and white fungus, mellow in taste, senile-resistant efficacy clearly, is arranged in pairs or groups with the beautiful ferment product of promise, i.e., convex
The anti-aging function of product has been shown, has also increased the mellow mouthfeel of the beautiful ferment product of promise.The formula collocation of the present invention is by changing
Kind mouthfeel is strengthened the theoretical of effect and is carried out, and a small amount of adhesive, embedding medium and bulk are only added in process of producing product
Agent, product mellow in taste, function are apparent.With the quickening of modern life rhythm and the raising of people's living standard, people
It is increasing to mellow and with health-care efficacy product demand.It is therefore it provides mellow and with health-care efficacy
The beautiful ferment product of promise has important practical significance, and more selections can be provided for consumer.
Preferably, in parts by weight, the dosage of each raw material is in the beautiful enzyme composition of promise：The beautiful ferment juice 200~400 of promise
Part, 20~50 parts of blueberry juice, 20~50 parts of Ginger P.E, 20~50 parts of red date extract, 20~50 parts of dried tremella extract.
Preferably, in parts by weight, the dosage of each raw material is in the beautiful enzyme composition of promise：280 parts of the beautiful ferment juice of promise, blueberry
40 parts of juice, 30 parts of Ginger P.E, 30 parts of red date extract, 30 parts of dried tremella extract.
Preferably, blueberry juice is the blueberry inspissated juice of solid content 50%~65%；
Ginger P.E is the Zingibers aqueous extract of solid content 20%~50%；
The jujube slurries that red date extract obtains after adding water mashing for jujube, decoct；
The white fungus slurries that dried tremella extract obtains after adding water mashing for white fungus, decoct.
Preferably, the solid content of the beautiful ferment juice of promise is 7%~10%, pH value is 2.5~4.0.
In specific embodiment provided by the invention, the solid content of the beautiful ferment juice of promise is 8%, pH value 3.0.
Preferably, the preparation method of blueberry juice is：Blueberries are squeezed the juice, are filtered, it is 50% to be concentrated into solid content
~65%, obtain blueberry juice.
In specific embodiment provided by the invention, the solid content of blueberry juice is 60%.
Preferably, the preparation method of Ginger P.E is：Ginger is taken, adds the water mashing of 6~12 times of amounts of ginger weight, adds
Heat maintains 30~90 minutes, filter to get filtrate A to 90 DEG C~100 DEG C；Filter residue adds the water of 6~10 times of amounts to stir evenly, and is heated to 90 DEG C
It~100 DEG C, maintains 30~90 minutes, filter to get filtrate B；Merging filtrate A and liquor B, be concentrated into solid content for 20%~
50%, obtain Ginger P.E.
In specific embodiment provided by the invention, the preparation method of Ginger P.E is：Ginger is taken, adds ginger weight 10
The water mashing of amount again, is heated to 100 DEG C, maintains 60 minutes, filter to get filtrate A；Filter residue adds the water of 8 times of amounts to stir evenly, and is heated to 100
DEG C, it maintains 60 minutes, filter to get filtrate B；Merging filtrate A and liquor B, it is 40% to be concentrated into solid content, obtains ginger and carries
Take object.
Preferably, the preparation method of red date extract is：Jujube is taken, adds the water mashing of 6~12 times of amounts of jujube weight, adds
Heat maintains 20~60 minutes, homogeneous obtains red date extract to 85 DEG C~95 DEG C.
In specific embodiment provided by the invention, the preparation method of red date extract is：Jujube is taken, adds jujube weight 10
The water mashing of amount again, is heated to 90 DEG C, maintains 40 minutes, homogeneous obtains red date extract.
Preferably, the preparation method of dried tremella extract is：White fungus is taken, adds the water mashing of 6~12 times of amounts of white fungus weight, adds
Heat maintains 20~60 minutes, homogeneous obtains dried tremella extract to 85 DEG C~95 DEG C.
In specific embodiment provided by the invention, the preparation method of dried tremella extract is：White fungus is taken, adds white fungus weight 10
The water mashing of amount again, is heated to 90 DEG C, maintains 40 minutes, homogeneous obtains dried tremella extract.
Preferably, the homogeneous in the preparation method of red date extract or dried tremella extract is using colloid mill homogeneous,
The number of homogeneous is 2 times.
There is the guarantor of anti-aging and/or strengthen immunity in preparation the present invention also provides the beautiful enzyme composition of the promise
Application in health food.
The present invention also provides a kind of beautiful enzyme preparation of promise, including the beautiful enzyme composition of promise provided by the invention and bromatology
Upper acceptable auxiliary material.
Preferably, the dosage form of the beautiful enzyme preparation of promise is pulvis or tablet.
Preferably, the raw material of pulvis or tablet includes：200~400 parts of the beautiful ferment juice of promise, 20~50 parts of blueberry juice are raw
20~50 parts of ginger extract, 20~50 parts of red date extract, 20~50 parts of dried tremella extract, 1~10 part of sodium carboxymethyl starch, two
0.5~5 part of silica, 3~15 parts of glucose syrup.
Preferably, the preparation method of pulvis is：
(1) the beautiful ferment juice of promise is taken, adds 0.1%~1.0% beta-cyclodextrin, is heated to 30~50 DEG C, maintains 30~60min,
Obtain the beautiful ferment cyclodextrin inclusion compound of promise；
Blueberries are squeezed the juice, are filtered, it is 50%~65% to be concentrated into solid content, obtains blueberry juice；
Ginger is taken, adds the water mashing of 6~12 times of amounts of ginger weight, is heated to 90 DEG C~100 DEG C, maintains 30~90 minutes,
Filter to get filtrate A；Filter residue adds the water of 6~10 times of amounts to stir evenly, and is heated to 90 DEG C~100 DEG C, maintains 30~90 minutes, filters to obtain filter
Liquid B；Merging filtrate A and liquor B, it is 20%~50% to be concentrated into solid content, obtains Ginger P.E；
Jujube is taken, adds the water mashing of 6~12 times of jujube weight amount, is heated to 85 DEG C~95 DEG C, maintains 20~60 minutes,
Matter obtains red date extract；
White fungus is taken, adds the water mashing of 6~12 times of white fungus weight amount, is heated to 85 DEG C~95 DEG C, maintains 20~60 minutes,
Matter obtains dried tremella extract；
(2) the beautiful ferment cyclodextrin inclusion compound of promise, blueberry juice, Ginger P.E, red date extract, dried tremella extract are mixed
It closes；
(3) sodium carboxymethyl starch, silica, glucose syrup are added in, is stirred evenly, is heated to 70~82 DEG C, maintain 5~
20 minutes；
(4) material of step (3) is taken to be dried in vacuo, is sieved, obtains the beautiful ferment pulvis of promise.
Preferably, the raw material of tablet further includes magnesium stearate and silica.
Preferably, in parts by weight, the raw material of tablet is：80~100 parts of the beautiful ferment pulvis of promise, magnesium stearate 1~8
Part, 0.5~5 part of silica.
Preferably, the preparation method of tablet is：The beautiful ferment pulvis of promise is taken, adds in magnesium stearate, silica, tabletting.
Vacuum drying condition in step (4) is：45 DEG C~65 DEG C of drying temperature, 90~99Kpa of vacuum degree.
The present invention provides the beautiful enzyme composition of promise and its preparations.The beautiful enzyme composition of the promise is by the beautiful ferment juice of promise, blueberry
Juice, Ginger P.E, red date extract and dried tremella extract composition.The technique effect that the present invention has is：
1st, the beautiful ferment juice of promise, blueberry juice, Ginger P.E, red date extract and dried tremella extract are used cooperatively by the present invention
Afterwards, significant synergistic function can be generated in terms of anti-aging, strengthen immunity；
2nd, the beautiful enzyme composition of promise provided by the invention and its preparation contain abundant nutritional ingredient, mellow, no promise
The intrinsic acid smell of beautiful ferment, mouthfeel is greatly improved, easy for consumers to accept.
Specific embodiment
The invention discloses the beautiful enzyme composition of promise and its preparation, those skilled in the art can use for reference present disclosure, fit
When modified technique parameter is realized.In particular, it should be pointed out that all similar substitutions and modifications are for a person skilled in the art
It is it will be apparent that they are considered as being included in the present invention.The present invention method and application by preferred embodiment into
Gone description, related personnel significantly can not depart from the content of present invention, in spirit and scope to method described herein and application
It is modified or suitably changes with combining, to realize and using the technology of the present invention.
Raw materials used or auxiliary material can be bought by market in the beautiful enzyme composition of promise provided by the invention and its preparation.
With reference to embodiment, the present invention is further explained：
Embodiment 1
(1) it stocks up
1. blueberry inspissated juice：Blueberries are taken, cleans, squeezes the juice, juice crosses 100 mesh filter-cloth filterings, is concentrated into solid content
It is 60%, it is spare.
2. ginger extract：Ginger is taken, is cleaned, adds the water mashing of 10 times of amounts of ginger weight, is heated to 100 DEG C, maintains 60
Minute, filtering obtains filtrate A；Filter residue adds the water of 8 times of amounts to stir evenly, and is heated to 100 DEG C, maintains 60 minutes, and filtering obtains liquor B；It closes
And filtrate A and B, it is 40% to be concentrated into solid content, spare.
3. jujube extract：Jujube is taken, is cleaned, adds the water mashing of 10 times of amounts of jujube weight, is heated to 90 DEG C, maintains 40 points
Twice of colloid mill is crossed in clock, cooling, spare.
4. tremella extract：White fungus is taken, is cleaned, adds the water mashing of 10 times of amounts of white fungus weight, is heated to 90 DEG C, maintains 40 points
Twice of colloid mill is crossed in clock, cooling, spare.
5. the beautiful ferment juice of promise is the fruit juice of purchase, solid content 8%, pH value 3.0.
(2) 280 parts of the beautiful ferment juice of promise, 40 parts of blueberry inspissated juice, 30 parts of ginger extract, 30 parts of jujube extract, silver are taken
30 parts of ear extracting solution, stirs evenly spare.
Embodiment 2
(1) it stocks up
With embodiment 1.
(2) 200 parts of the beautiful ferment juice of promise, 50 parts of blueberry inspissated juice, 20 parts of ginger extract, 50 parts of jujube extract, silver are taken
20 parts of ear extracting solution, stirs evenly spare.
Embodiment 3
(1) it stocks up
With embodiment 1.
(2) 400 parts of the beautiful ferment juice of promise, 20 parts of blueberry inspissated juice, 50 parts of ginger extract, 20 parts of jujube extract, silver are taken
50 parts of ear extracting solution, stirs evenly spare.
Comparative example 1
(1) it stocks up
With the preparation of embodiment 1 ginger extract, jujube extract, tremella extract.
(2) 50 parts of ginger extract, 20 parts of jujube extract, 30 parts of tremella extract are taken, is stirred evenly spare.
Comparative example 2
(1) it stocks up
With the preparation of the beautiful ferment juice of 1 promise of embodiment and blueberry inspissated juice.
(2) 200 parts of the beautiful ferment juice of promise is taken, 30 parts of blueberry inspissated juice stirs evenly spare.
The beautiful enzyme composition anti-aging of 1 promise of test example and strengthen immunity function test
1. test material
1.1 given the test agent
1-3 of the embodiment of the present invention and the beautiful enzyme composition of promise of comparative example 1-2 techniques preparation are taken as test specimen.
1.2 test dose
Embodiment 1-3 and comparative example 1-2 sample dosages are 30ml/d, and in terms of the weight 60kg that is grown up, average dose is
500mg/ (kgd), i.e. test specimen dosage are 500mg/ (kgd).
The present embodiment sets basic, normal, high 3 dosage groups of test specimen, be set to 500,1000,2000mg/ (kgd).
1.3 experimental animal
Kunming mice, 18~22g, Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center provide, and normal raising supplies examination after 3 days.
1.4 key instruments and reagent
UV-2501 ultraviolet-uisible spectrophotometers (Japanese Shimadzu Corporation)；(Sartorius is public for BS110S electronic balances
Department)；XZC-5A type mouse diving towers instrument (Jiangsu Sai Angsi bio tech ltd)；D- galactolipins；SOD reagents；MDA reagents；
Coomassie brilliant blue reagent；Syklofosfamid ampoule.
2. test method
The foundation of 2.1 aging models and grouping administration
Take kunming mice 108, half male and half female, be randomly divided into blank control group, negative control group, embodiment 1 it is high, in,
Low dose group, 2 groups of embodiment, 3 groups of embodiment, 1 group of comparative example, 2 groups of comparative example, every group of 12 kunming mices.In aseptic condition
Under, in addition to blank control group, remaining each group mouse subcutaneously notes 4%D- galactolipin physiology by 10.0mL/kg dosage in nape part
Salt-water liquid, one time a day, continuous injection 6 weeks cause aging model, and blank control group is subcutaneously injected isometric sterile 0.9%
Sodium chloride solution.Modeling gives test specimen, blank control group and the daily gavage of negative control group by corresponding dosage gavage simultaneously
Respective volume distilled water, daily gastric infusion 1 time, continuous 6 weeks.
The foundation of 2.2 immunodeficiency models and grouping administration
Take kunming mice 108, half male and half female, be randomly divided into blank control group, negative control group, embodiment 1 it is high, in,
Low dose group, 2 groups of embodiment, 3 groups of embodiment, 1 group of comparative example, 2 groups of comparative example, every group 12.In addition to blank control group, remaining
Each group mouse pressed 50.0mg/kg intraperitoneal injection of cyclophosphamide parenteral solutions in the 1st, 3,5 day.Modeling is given simultaneously by corresponding dosage gavage
Give test specimen, blank control group and the daily gavage respective volume distilled water of negative control group, daily gastric infusion 1 time, continuously
14d。
2.3 observation index
2.3.1 aging model
6 weeks appearances for observing and recording mouse are administered in modeling, and learn note using step dow n test test mouse within the 40th day in administration
Recall ability, record the errors number in the 1st incubation period and 10 minutes for jumping off platform of mouse.1h after administration in 42nd day, mouse
It weighs, eye socket takes blood and detaches serum, measures superoxide dismutase activity in serum；After take mouse brain and liver, add 0.9%
10% brain and liver tissues homogenate is made in sodium chloride solution, measures wherein mda content, while even with biuret method measure tissue
Protein content is starched, and takes spleen and thymus gland, calculates Thymus and Spleen index.
2.3.2 immunosuppression mouse model
In 1h after the last administration, mouse is weighed, 25% Chinese ink physiological salt liquid 10.0mL/kg of tail vein injection, in
2 minutes and 12 minutes after injection prepared Chinese ink, blood 2-3 drops are taken from mouse orbit rear vein beard with capillary glass-tube, 20.0 μ l is inhaled and is dissolved in
2.0mL 0.1%Na2CO3It in solution, shakes up, surveys absorbance at 680nm under 721 type spectrophotometrics, gulped down by following equation calculating
Bite index K.Spleen and thymus gland are taken simultaneously, calculate thymus gland, Spleen coefficient.
Using more comparison among groups using one-way analysis of variance, experimental data uses t check analysis methods, compares test data
Group difference, data are represented with x ± s.
3. result
Influence of the beautiful ferment of 3.1 promises to mouse aging learning and memory
Continuously to D- galactolipins after 6 weeks, negative control group mouse hair color is withered and yellow, matt, is slow in action, and presents apparent
Aging sign.
In Jumping test, the electric shock incubation period of negative control group mouse declines, and errors number increases, with blank control group
It compares, negative control group mouse electric shock incubation period is decreased obviously, the statistically significant (P of difference<0.01), illustrate that D- galactolipins are long
Phase administration causes the learning and memory of mouse certain injury.
The electric shock incubation period that the beautiful enzyme composition test specimen successive administration of promise can extend mouse in 6 weeks, and reduce mistake
Number, the ability of learning and memory that it is prompted to have the function of to improve mouse aging.It the results are shown in Table 1.
Influence (x ± s) of the beautiful enzyme composition of 1 promise of table to mouse aging learning and memory
Note：Compared with blank control group,*P<0.05,**P<0.01；Compared with negative control group,#P<0.05,##P<0.01.
Influence of the beautiful ferment of 3.2 promises to mouse aging immune organ weight index and SOD in serum
Compared with blank control group, the apparent atrophy of negative control group mouse thymus, spleen, SOD in serum level are remarkably decreased,
And the beautiful each dosage group of enzyme composition test specimen of promise can increase the thymus gland coefficient of mouse aging, effectively antagonize to D- galactolipins
The decline of mouse aging SOD in serum level has an impact the Spleen coefficient of mouse aging.It the results are shown in Table 2.
Influence (x ± s) of the beautiful enzyme composition of 2 promise of table to mouse aging organ coefficient and SOD in serum
Note：Compared with blank control group,**P<0.01；Compared with negative control group,#P<0.05
Influence of the beautiful ferment of 3.3 promises to mouse aging liver, brain tissue MDA levels
Compared with blank control group, the MDA contents in negative control group Mouse Liver, brain tissue significantly increase (P<0.01 or
0.05), the beautiful each dosage group of enzyme composition test specimen of promise can be substantially reduced the MDA contents (P in liver<0.05), brain tissue
The reduction level of middle MDA is with high, middle dose group (P with obvious effects<0.05).It the results are shown in Table 3.
Influence (x ± s) of the beautiful enzyme composition of 3 promise of table to mouse aging liver and brain tissue MDA levels
Note：Compared with blank control group,*P<0.05,**P<0.01；Compared with negative control group,#P<0.05。
Influence of the beautiful enzyme composition of 3.4 promises to organ weight, mononuclear-macrophage phagocytic function
Compared with blank group, thymus gland coefficient, Spleen coefficient and the phagocyte imdex K of negative control group mouse are significantly reduced
(P<0.01)；And the beautiful each dosage group of enzyme composition test specimen of promise can significantly improve caused by cyclophosphamide hypoimmunity mice
Spleen coefficient and phagocyte imdex (P<0.01 or 0.05), while high, middle dose group can significantly improve hypoimmunity mice
Thymus gland coefficient (P<0.01 or 0.05), prompt the beautiful enzyme composition test specimen of promise that can enhance its immune function, and with carrying
The effect of the nonspecific phagocytosis of high mouse reticuloendothelial system.It the results are shown in Table 4.
Influence (x ± s) of the beautiful enzyme composition of 4 promise of table to hypoimmunity mice
Note：Compared with blank control group,*P<0.01；Compared with negative control group,##P<0.01,#P<0.05。
3.5 analysis
The present embodiment uses classical aged animal model caused by mouse long term injections D- galactolipins, and decline cyclophosphamide pair
Mouse is formed under immunosuppressive experiment condition, the phagocyte imdex of each dosage group mouse of the beautiful enzyme composition test specimen of promise
It is significantly higher than negative control group, i.e. Function of mononuclear phagocyte is remarkably reinforced, and illustrates that it can enhance the nospecific immunity of body
Function.Meanwhile the beautiful enzyme composition test specimen of promise can increase mouse thymus coefficient and Spleen coefficient.
The present embodiment has rated the beauty and health care function of the beautiful enzyme composition of promise in terms of anti-aging and immunological regulation two, card
Bright its can be played a role by inhibiting oxidation in aging course, improving function of immune system etc..
The beautiful ferment pulvis of 4 promise of embodiment
(1) 200 parts of the beautiful ferment juice of promise is taken, adds 0.1% beta-cyclodextrin, is heated to 40 DEG C, maintains 60min, it is spare.
(2) add in blueberry inspissated juice 30 parts of (solid content 60%), 50 parts of ginger extract (solid content 40%),
20 parts of jujube extract, 30 parts of tremella extract, stir evenly spare.
1. blueberry inspissated juice：Blueberries are taken, cleans, squeezes the juice, juice crosses 80-180 mesh filter-cloth filterings, is concentrated into solid content and contains
It is 60% to measure, spare.
2. ginger extract：Ginger is taken, is cleaned, adds the water mashing of 10 times of amounts of ginger weight, is heated to 100 DEG C, maintains 60
Minute, filtering obtains filtrate A；Filter residue adds the water of 8 times of amounts to stir evenly, and is heated to 100 DEG C, maintains 60 minutes, and filtering obtains liquor B；It closes
And filtrate A and B, it is 40% to be concentrated into solid content, spare.
3. jujube extract：Jujube is taken, is cleaned, adds the water mashing of 10 times of amounts of jujube weight, is heated to 90 DEG C, maintains 40 points
Twice of colloid mill is crossed in clock, cooling, spare.
4. tremella extract：White fungus is taken, is cleaned, adds the water mashing of 10 times of amounts of white fungus weight, is heated to 90 DEG C, maintains 40 points
Twice of colloid mill is crossed in clock, cooling, spare.
(3) 5 parts of sodium carboxymethyl starch, 5 parts of silica, 10 parts of glucose syrup are added in, stirs evenly, is heated to 80 DEG C,
It maintains 10 minutes, it is spare.
(4) material of step (3) is taken into vacuum belt drier, 45 DEG C -65 DEG C, vacuum degree 90-99Kpa of drying temperature,
20-50 mesh sieve is crossed, get Nuo Li enzyme preparation original powders are spare.
(5) the beautiful ferment original powder of promise is taken, is packed as 4.0g/ bags of solid beverage, the as beautiful ferment solid granules of promise.
The beautiful ferment tablet of 5 promise of embodiment
80-100 parts of 4 promise of Example beautiful ferment original powder adds in 1-8 parts of magnesium stearate, adds in 0.5-5 parts of silica,
The tablet of 0.5-1.0g/ pieces, the as beautiful enzyme film of promise are pressed into, the beautiful enzyme film of promise is unique in taste, is easily received by consumer.
The beautiful enzyme preparation anti-aging of 2 promise of test example and strengthen immunity function test
1. test material
1.1 given the test agent
The beautiful enzyme preparation 4.0g of promise that 4 technique of the embodiment of the present invention is taken to prepare, is configured to 10mL test specimens.
1.2 test dose
4 sample dosage of embodiment is 4.0g/d, and in terms of the weight 60kg that is grown up, average dose is 66.7mg/ (kgd), i.e.,
Test specimen dosage is 166mg/ (kgd).
The present embodiment sets basic, normal, high 3 dosage groups of test specimen, be set to 830,1660,3320mg/ (kgd).
1.3 experimental animal
Kunming mice, 18~22g, Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center provide, and normal raising supplies examination after 3 days.
1.4 key instruments and reagent
UV-2501 ultraviolet-uisible spectrophotometers (Japanese Shimadzu Corporation)；(Sartorius is public for BS110S electronic balances
Department)；XZC-5A type mouse diving towers instrument (Jiangsu Sai Angsi bio tech ltd)；D- galactolipins；SOD reagents；MDA reagents；
Coomassie brilliant blue reagent；Syklofosfamid ampoule.
2. test method
The foundation of 2.1 aging models and grouping administration
Kunming mice 60 is taken, it is high, medium and low to be randomly divided into blank control group, negative control group, embodiment 4 for half male and half female
Dosage group, every group of 12 kunming mices.Aseptically, in addition to blank control group, remaining each group mouse presses 10.0mL/kg
Dosage subcutaneously notes 4%D- galactolipin physiological salt liquids in nape part, and one time a day, continuous injection 6 weeks causes aging model,
Isometric sterile 0.9% sodium chloride solution is subcutaneously injected in blank control group.Modeling gives test sample by corresponding dosage gavage simultaneously
Product, blank control group and the daily gavage respective volume distilled water of negative control group, daily gastric infusion 1 time, continuous 6 weeks.
The foundation of 2.2 immunodeficiency models and grouping administration
Kunming mice 60 is taken, it is high, medium and low to be randomly divided into blank control group, negative control group, embodiment 4 for half male and half female
Dosage group, every group 12.In addition to blank control group, ring phosphorus was injected intraperitoneally by 50.0mg/kg in the 1st, 3,5 day in remaining each group mouse
Amide parenteral solution.Modeling gives test specimen, blank control group and the daily gavage of negative control group by corresponding dosage gavage simultaneously
Respective volume distilled water, daily gastric infusion 1 time, continuous 14d.
2.3 observation index
2.3.1 aging model
6 weeks appearances for observing and recording mouse are administered in modeling, and learn note using step dow n test test mouse within the 40th day in administration
Recall ability, record the errors number in the 1st incubation period and 10 minutes for jumping off platform of mouse.1h after administration in 42nd day, mouse
It weighs, eye socket takes blood and detaches serum, measures superoxide dismutase activity in serum；After take mouse brain and liver, add 0.9%
10% brain and liver tissues homogenate is made in sodium chloride solution, measures wherein mda content, while even with biuret method measure tissue
Protein content is starched, and takes spleen and thymus gland, calculates Thymus and Spleen index.
2.3.2 immunosuppression mouse model
In 1h after the last administration, mouse is weighed, 25% Chinese ink physiological salt liquid 10.0mL/kg of tail vein injection, in
2 minutes and 12 minutes after injection prepared Chinese ink, blood 2-3 drops are taken from mouse orbit rear vein beard with capillary glass-tube, 20.0 μ l is inhaled and is dissolved in
2.0mL 0.1%Na2CO3It in solution, shakes up, surveys absorbance at 680nm under 721 type spectrophotometrics, gulped down by following equation calculating
Bite index K.Spleen and thymus gland are taken simultaneously, calculate thymus gland, Spleen coefficient.
Using more comparison among groups using one-way analysis of variance, experimental data uses t check analysis methods, compares test data
Group difference, data are represented with x ± s.
3. result
Influence of the beautiful ferment of 3.1 promises to mouse aging learning and memory
Continuously to D- galactolipins after 6 weeks, negative control group mouse hair color is withered and yellow, matt, is slow in action, and presents apparent
Aging sign.
In Jumping test, the electric shock incubation period of negative control group mouse declines, and errors number increases, with blank control group
It compares, negative control group mouse electric shock incubation period is decreased obviously, the statistically significant (P of difference<0.01), illustrate that D- galactolipins are long
Phase administration causes the learning and memory of mouse certain injury.
The electric shock incubation period that the beautiful ferment original powder test specimen successive administration of promise can extend mouse in 6 weeks, and reduce wrong time
Number, the ability of learning and memory that it is prompted to have the function of to improve mouse aging.It the results are shown in Table 5.
Influence (x ± s) of the beautiful ferment original powder of 5 promise of table to mouse aging learning and memory
Note：Compared with blank control group,*P<0.05,**P<0.01
Influence of the beautiful ferment of 3.2 promises to mouse aging immune organ weight index and SOD in serum
Compared with blank control group, the apparent atrophy of negative control group mouse thymus, spleen, SOD in serum level are remarkably decreased,
And the beautiful each dosage group of ferment original powder test specimen of promise can increase the thymus gland coefficient of mouse aging, effectively antagonize and decline to D- galactolipins
The decline of grown-ups and children mouse SOD in serum level, has an impact the Spleen coefficient of mouse aging.It the results are shown in Table 6.
Influence (x ± s) of the beautiful ferment original powder of 6 promise of table to mouse aging organ coefficient and SOD in serum
Note：Compared with blank control group,**P<0.01；Compared with negative control group,#P<0.05
Influence of the beautiful ferment of 3.3 promises to mouse aging liver, brain tissue MDA levels
Compared with blank control group, the MDA contents in negative control group Mouse Liver, brain tissue significantly increase (P<0.01 or
0.05), the beautiful each dosage group of ferment original powder test specimen of promise can be substantially reduced the MDA contents (P in liver<0.05), in brain tissue
The reduction level of MDA is with high, middle dose group (P with obvious effects<0.05).It the results are shown in Table 7.
Influence (x ± s) of the beautiful ferment original powder of 7 promise of table to mouse aging liver and brain tissue MDA levels
Note：Compared with blank control group,*P<0.05,**P<0.01；Compared with negative control group,#P<0.05。
Influence of the beautiful ferment original powder of 3.4 promises to organ weight, mononuclear-macrophage phagocytic function
Compared with blank group, thymus gland coefficient, Spleen coefficient and the phagocyte imdex K of negative control group mouse are significantly reduced
(P<0.01)；And the beautiful each dosage group of ferment original powder test specimen of promise can significantly improve caused by cyclophosphamide hypoimmunity mice
Spleen coefficient and phagocyte imdex (P<0.01 or 0.05), while high, middle dose group can significantly improve hypoimmunity mice
Thymus gland coefficient (P<0.01 or 0.05), prompt the beautiful ferment original powder test specimen of promise that can enhance its immune function, and small with improving
The effect of the nonspecific phagocytosis of mouse reticuloendothelial system.It the results are shown in Table 8.
Influence (x ± s) of the beautiful ferment original powder of 8 promise of table to hypoimmunity mice
Note：Compared with blank control group, * P<0.01；Compared with negative control group, ##P<0.01, #P<0.05.
3.5 analysis
The present embodiment uses classical aged animal model caused by mouse long term injections D- galactolipins, and decline cyclophosphamide pair
Mouse is formed under immunosuppressive experiment condition, and the phagocyte imdex of each dosage group mouse of the beautiful ferment original powder test specimen of promise is shown
It writes higher than negative control group, i.e. Function of mononuclear phagocyte is remarkably reinforced, and illustrates that it can enhance the nospecific immunity work(of body
Energy.Meanwhile the beautiful ferment original powder test specimen of promise can increase mouse thymus coefficient and Spleen coefficient.
The present embodiment has rated the beauty and health care function of the beautiful ferment original powder of promise in terms of anti-aging and immunological regulation two, it was demonstrated that
It can be played a role by inhibiting oxidation in aging course, improving function of immune system etc..
3 mouthfeel evaluation test of test example
Mouthfeel evaluation is carried out to the product of embodiment 1-4 and comparative example 1-2, evaluation result is as follows：
9 mouthfeel evaluation result of table
Result of the test shows this product mellow in taste, the beautiful acid smell of of no promise itself, and mouthfeel is greatly improved, and is easily consumption
Person receives.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of beautiful enzyme composition of promise, which is characterized in that by the beautiful ferment juice of promise, blueberry juice, Ginger P.E, red date extract
It is formed with dried tremella extract.
2. the beautiful enzyme composition of promise according to claim 1, which is characterized in that in parts by weight, the beautiful ferment group of promise
The dosage of each raw material is in conjunction object：200~400 parts of the beautiful ferment juice of promise, 20~50 parts of blueberry juice, 20~50 parts of Ginger P.E,
20~50 parts of red date extract, 20~50 parts of dried tremella extract.
3. the beautiful enzyme composition of promise according to claim 1, which is characterized in that in parts by weight, the beautiful ferment group of promise
The dosage of each raw material is in conjunction object：280 parts of the beautiful ferment juice of promise, 40 parts of blueberry juice, 30 parts of Ginger P.E, red date extract 30
Part, 30 parts of dried tremella extract.
4. the beautiful enzyme composition of promise according to claim 1, which is characterized in that the blueberry juice is solid content 50%
~65% blueberry inspissated juice；
The Ginger P.E is the Zingibers aqueous extract of solid content 20%~50%；
The jujube slurries that the red date extract obtains after adding water mashing for jujube, decoct；
The white fungus slurries that the dried tremella extract obtains after adding water mashing for white fungus, decoct.
5. the beautiful enzyme composition of the promise as described in any one of claims 1 to 4 is being prepared with anti-aging and/or strengthen immunity
Application in the health food of effect.
6. a kind of beautiful enzyme preparation of promise, which is characterized in that including the beautiful ferment combination of the promise as described in any one of claims 1 to 4
Acceptable auxiliary material on object and bromatology.
7. the beautiful enzyme preparation of promise according to claim 6, which is characterized in that the dosage form of the beautiful enzyme preparation of promise is pulvis
Or tablet.
8. the beautiful enzyme preparation of promise according to claim 7, which is characterized in that the raw material of the pulvis or tablet includes：Promise
Beautiful 200~400 parts of ferment juice, 20~50 parts of blueberry juice, 20~50 parts of Ginger P.E, 20~50 parts of red date extract, white fungus
20~50 parts of extract, 1~10 part of sodium carboxymethyl starch, 0.5~5 part of silica, 3~15 parts of glucose syrup.
9. the beautiful enzyme preparation of promise according to claim 8, which is characterized in that the preparation method of the pulvis is：
(1) the beautiful ferment juice of promise is taken, adds 0.1%~1.0% beta-cyclodextrin, is heated to 30~50 DEG C, 30~60min is maintained, obtains
The beautiful ferment cyclodextrin inclusion compound of promise；
Blueberries are squeezed the juice, are filtered, it is 50%~65% to be concentrated into solid content, obtains blueberry juice；
Ginger is taken, adds the water mashing of 6~12 times of amounts of ginger weight, is heated to 90 DEG C~100 DEG C, maintains 30~90 minutes, filtering
Obtain filtrate A；Filter residue adds the water of 6~10 times of amounts to stir evenly, and is heated to 90 DEG C~100 DEG C, maintains 30~90 minutes, filter to get filtrate B；
Merging filtrate A and liquor B, it is 20%~50% to be concentrated into solid content, obtains Ginger P.E；
Jujube is taken, adds the water mashing of 6~12 times of amounts of jujube weight, is heated to 85 DEG C~95 DEG C, maintains 20~60 minutes, homogeneous,
Obtain red date extract；
White fungus is taken, adds the water mashing of 6~12 times of amounts of white fungus weight, is heated to 85 DEG C~95 DEG C, maintains 20~60 minutes, homogeneous,
Obtain dried tremella extract；
(2) the beautiful ferment cyclodextrin inclusion compound of promise, blueberry juice, Ginger P.E, red date extract, dried tremella extract are mixed；
(3) sodium carboxymethyl starch, silica, glucose syrup are added in, is stirred evenly, is heated to 70~82 DEG C, maintains 5~20 points
Clock；
(4) material of step (3) is taken to be dried in vacuo, is sieved, obtains the beautiful ferment pulvis of promise.
10. the beautiful enzyme preparation of promise according to claim 8, which is characterized in that the raw material of the tablet further includes stearic acid
Magnesium and silica.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109198617A (en) * | 2018-09-12 | 2019-01-15 | 海南热泉食品贸易有限公司 | A kind of beautiful ferment extraction process of promise |
| CN109480152A (en) * | 2018-11-07 | 2019-03-19 | 河南应天养生保健有限公司 | A kind of functional beverage for adjusting taste lower hyperlipidemia, hypertension, hyperglycemia to improve immunity |
| CN109619348A (en) * | 2019-01-04 | 2019-04-16 | 湖南海济药业有限公司 | A kind of antioxidative enzyme beverage with rich flavor and preparation method thereof |
| CN110384121A (en) * | 2019-07-26 | 2019-10-29 | 中国热带农业科学院热带作物品种资源研究所 | A kind of cake and preparation method thereof beautiful containing promise |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109198617A (en) * | 2018-09-12 | 2019-01-15 | 海南热泉食品贸易有限公司 | A kind of beautiful ferment extraction process of promise |
| CN109480152A (en) * | 2018-11-07 | 2019-03-19 | 河南应天养生保健有限公司 | A kind of functional beverage for adjusting taste lower hyperlipidemia, hypertension, hyperglycemia to improve immunity |
| CN111149955A (en) * | 2018-11-07 | 2020-05-15 | 海南松吉云商科技有限公司 | Morinda citrifolia enzyme flavored beverage and preparation method thereof |
| CN111149904A (en) * | 2018-11-07 | 2020-05-15 | 海南松吉云商科技有限公司 | Morinda citrifolia enzyme tablet candy and making method thereof |
| CN109619348A (en) * | 2019-01-04 | 2019-04-16 | 湖南海济药业有限公司 | A kind of antioxidative enzyme beverage with rich flavor and preparation method thereof |
| CN110384121A (en) * | 2019-07-26 | 2019-10-29 | 中国热带农业科学院热带作物品种资源研究所 | A kind of cake and preparation method thereof beautiful containing promise |
| CN110384121B (en) * | 2019-07-26 | 2023-03-10 | 中国热带农业科学院热带作物品种资源研究所 | Cake containing noni and preparation method thereof |
| CN110916028A (en) * | 2019-12-20 | 2020-03-27 | 刘枫 | Noni composite enzyme fruit juice and preparation method thereof |
| CN112715809A (en) * | 2021-01-05 | 2021-04-30 | 晋江市宏品优创贸易有限公司 | Fruit juice for dispelling effects of alcohol and protecting liver |
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