CN110463962A - A kind of marine protein oligopeptide health powder - Google Patents

A kind of marine protein oligopeptide health powder Download PDF

Info

Publication number
CN110463962A
CN110463962A CN201910910186.7A CN201910910186A CN110463962A CN 110463962 A CN110463962 A CN 110463962A CN 201910910186 A CN201910910186 A CN 201910910186A CN 110463962 A CN110463962 A CN 110463962A
Authority
CN
China
Prior art keywords
parts
peptide
health
oligopeptide
health powder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910910186.7A
Other languages
Chinese (zh)
Inventor
于海桥
赵雅琳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rongcheng Ocean Oasis Biotechnology Co Ltd
Original Assignee
Rongcheng Ocean Oasis Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rongcheng Ocean Oasis Biotechnology Co Ltd filed Critical Rongcheng Ocean Oasis Biotechnology Co Ltd
Priority to CN201910910186.7A priority Critical patent/CN110463962A/en
Publication of CN110463962A publication Critical patent/CN110463962A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00—Soups; Sauces; Preparation or treatment thereof
    • A23L23/10—Soup concentrates, e.g. powders or cakes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/20—Agglomerating; Granulating; Tabletting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention relates to health preserving soup product and field of health care products, more particularly to a kind of albumen oligopeptide health powder, including peptides, dietary fiber class and seasoning class natural food materials, peptides are the oligopeptide extracted in marine product, and peptides include that perhaps oyster peptide dietary fiber class includes inulin or oligofructose for sea cucumber peptide, abalone peptide, krill peptide, seasoning class includes purple perilla or sweet basil, the parts by weight composition of food materials is as follows: 70-180 parts of peptides, 120-180 parts of dietary fiber class, 10-30 parts of seasoning class.The albumen oligopeptide health powder uses natural seafood food materials, without using additive, delicate flavour is strong, soluble good, nutritive value is high, is conducive to the absorption rate for improving protein, nutritional supplementation as dietary protein, the proteinaceous nutrient demand that can guarantee the crowd of sub-health population and digestion function of stomach and intestine discomfort, has the effect of good antifatigue, is suitble to the weaker crowd and the middle-aged and the old of constitution edible.

Description

A kind of marine protein oligopeptide health powder
Technical field
The present invention relates to health preserving soup product and nutrition and health care diet fields, and in particular to a kind of marine protein oligopeptide health preserving soup Powder.
Background technique
Protein is the basis of life, and Nutritional studies discovery, it is with amino that the protein of absorption of human body, which has one third, The form of acid absorbs, have three/second is that being absorbed in the form of oligopeptide.Oligopeptide is the predigestion product of protein, is by 2- The segment of 10 Amino acid profiles, molecular weight are not required to digestion or slightly digestion are i.e. absorbable less than 1000 dalton.People take the photograph It takes the mode of supplement protein constantly changing always, from natural foods such as direct human consumption soybean, milk, is transitioned into simple to its It is processed into the concentration essence such as soymilk, milk powder, then finally supplements protein by eating various protein powders.In recent years messenger Protein is changed into the form of oligopeptide, not only significantly improve nutritive value, human body physiological function can also be adjusted, had Beneficial to maintaining human health level, the physiological regulation function of the absorption characteristic and many aspects easy to digest of oligopeptide is more established Its basic status in the formula food of nutritional intervention.It can be by food egg currently with modern biotechnology zymolysis technique White enzymatic hydrolysis has been realized in industrialization at the small molecule oligopeptide for being easy to absorb.
Living marine resources are unique, evolution of the marine organisms under hypertonic, low temperature, low-oxygen environment, so that they are gathered around There are the genome different from terrestrial life, metabolic rule and degeneration-resistant means, forms a series of configurations, unique properties, tool There is the bioactive natural product of huge applications potentiality.Marine organisms can generate the special protein of many functions during evolution, Abstraction function albumen and sp act peptide in marine organisms, it has also become countries in the world health care product, development of functional food it is important Field.But at present in the field of health food and functional food, popular is essentially all the product of a kind of compounding, and peptides add The herbaceous plant of integration of drinking and medicinal herbs, being fabricated to form is health functional food class tablet or capsule, and this kind of product is expensive, For consumers, cost performance is relatively low.And existing market lacks in terms of food-borne marine oligopeptide food Development and application, without preferably embodying the advantage and effect of marine oligopeptide this kind of product.
Summary of the invention
It is an object of the invention to solve the above problem present in the prior art and application link, a kind of ocean is proposed Its application field is placed on nutrition by albumen oligopeptide health powder, taste and characteristic for food-borne marine oligopeptide itself On the carrier of soup, the characteristic and function of raw material are preferably embodied, using natural fresh Studies of The Aromatic Substances, does not use additive, but it is suitable It answers the fresh salty smell of raw material itself directly to enter soup and achievees the effect that same delicious, be conducive to the absorption rate for improving protein, It is more favorable to guarantee the proteinaceous nutrient demand of sub-health population, improves its health.
The technical scheme is that
A kind of marine protein oligopeptide health powder, including peptides, dietary fiber class and seasoning class natural food materials, it is described Peptides are the oligopeptide extracted in marine product, and the peptides include sea cucumber peptide, abalone peptide, krill peptide or oyster peptide, the meals Eating fiber-like includes that perhaps the oligofructose seasoning class includes purple perilla or sweet basil, the parts by weight group of the food materials to inulin At as follows: 70-180 parts of peptides, 120-180 parts of dietary fiber class, 10-30 parts of seasoning class.
What peptides raw material was selected is the small oligopeptide of molecular weight, and as seen from Figure 1, molecular weight is low less than 1000 dalton Poly- peptide accounting is 90% or more.The small oligopeptide of molecular weight is can absorb without digesting or slightly digesting, with other albumen with food When, oligopeptide can be first absorbed, then digest and assimilate other albumen, form a Gradient Absorption, preferably meet the albumen need of human body It asks.
Further, the component including following parts by weight: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, krill peptide 60-140 Part, 120-180 parts of inulin, 10-30 parts of sweet basil.
Further, the component including following parts by weight: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, oyster peptide 60-140 Part, 120-180 parts of inulin, 10-30 parts of purple perilla.
Further, the component including following parts by weight: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, krill peptide 60-140 Part, 120-180 parts of oligofructose, 10-30 parts of purple perilla.
Further, the component including following parts by weight: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, oyster peptide 60-140 Part, 120-180 parts of oligofructose, 10-30 parts of sweet basil.
Further, the component including following parts by weight: 10 parts of sea cucumber peptide, 10 parts of abalone peptide, 130 parts of krill peptide, inulin 140 parts, 10 parts of purple perilla.
Further, the component including following parts by weight: 10 parts of sea cucumber peptide, 10 parts of abalone peptide, 130 parts of oyster peptide, oligomeric 140 parts of fructose, 10 parts of sweet basil.
Further, the health powder further includes B family vitamin class component.
Further, the marine product further includes fish or scallop seafood.
Further, the preparation step of the health powder is as follows:
(1) after being homogenized selected seafood meat, pepsin is sequentially added into homogenate and trypsase is digested, Obtain enzymolysis liquid, after enzymatic hydrolysis under the conditions of 100 DEG C enzyme deactivation 15min；Obtained enzymolysis liquid is centrifuged removal upper layer floating material And bottom sediment, retain supernatant；Obtained supernatant is centrifuged to removal precipitating again, and is selected super no more than 10000Da Filter membrane carries out ultra filtering clarifying, and obtained ultrafiltrate is spray-dried, marine oligopeptide powder is prepared；
Wherein, the molecular cut off of ultrafiltration membrane is 2000Da；Preferably, the molecular cut off of ultrafiltration membrane is no more than 1000Da；
(2) the oligopeptide powder of each marine product in health preserving soup powder component is prepared respectively using step (1) method, then basis The parts by weight of each component distinguish accurate weighing each component, are crushed after mixing；
(3) smashed raw material is crossed into 80 meshes, health powder is prepared.
Further, the health powder is for making soup product or directly water being added to drink.
Further, the health powder is prepared into solid particle, and health powder is prepared into the form of solid particle, In When being dispensed, it can make soup product according to the difference of edible way or add the weight of water required health powder when directly drinking For the difference of amount, it is specified that one is packed the quantity of endoparticle, divider lattice packaging facilitates the edible needs of people.
The preparation method of the particle the following steps are included:
(1) the health powder being prepared is added in V-Mixer and is mixed 10-15 minutes, 95% ethanol solution is added Make bonding wetting agent, continues stirring and softwood is made, pelletized with 16 mesh of oscillating granulator；
(2) particle made is entered into boiling drier, drying temperature is 40-55 DEG C, and pellet moisture control is on 2% left side It is right；By 12 mesh sieves of the particle of drying；
(3) particle obtained dispensed, packed, obtain finished product.
Beneficial effects of the present invention:
(1) albumen oligopeptide health powder provided by the invention uses natural seafood food materials, reachable without using additive To the effect of same fresh perfume, oligomeric peptide molecular weight is small, i.e. absorbable without digestion or slightly digestion, can with other albumen with eating Oligopeptide is first absorbed, then digests and assimilates other albumen, forms a Gradient Absorption, preferably meets the albumen demand of human body.Make For the nutritional supplementation of dietary protein, it is ensured that the proteinaceous nutrient of the crowd of sub-health population and digestion function of stomach and intestine discomfort Demand, and the health preserving soup also has the effect of good antifatigue, is suitble to the weaker crowd and the middle-aged and the old of constitution edible.
(2) albumen oligopeptide health powder provided by the invention can be more preferable compared to conventional tablet or capsule form Embodiment marine oligopeptide substance nutritive peculiarity；Product delicate flavour is strong, and soluble good, soup look is limpid, and nutritive value is high；System It is standby to help to pack at the form of particle, the quantity of a packaging endoparticle powder is determined according to amount, facilitates people edible.
Detailed description of the invention
Fig. 1 is the graph of molecular weight distribution of sea cucumber peptide provided by the invention；
Fig. 2 is the data point of influence of the albumen oligopeptide health powder (TYS) to mouse swimming time in test example Analysis figure；
Fig. 3 is the data analysis of influence of the albumen oligopeptide health powder (TYS) to mouse hepatic glycogen content in test example Figure.
Specific embodiment
Clear, complete description is carried out to technical solution of the present invention below in conjunction with specific embodiment, it is clear that described Embodiment be only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ability Without making creative work, every other embodiment obtained belongs to this hair to domain those of ordinary skill The range of bright protection.
For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Embodiment 1
A kind of marine protein oligopeptide health powder, the raw material including following parts by weight: 5 parts of sea cucumber peptide, 5 parts of abalone peptide, 60 parts of krill peptide, 120 parts of inulin, 10 parts of sweet basil.
Embodiment 2
A kind of marine protein oligopeptide health powder, the raw material including following parts by weight: 20 parts of sea cucumber peptide, abalone peptide 20 Part, 140 parts of oyster peptide, 180 parts of inulin, 30 parts of purple perilla.
Embodiment 3
A kind of marine protein oligopeptide health powder, the raw material including following parts by weight: 10 parts of sea cucumber peptide, abalone peptide 10 Part, 100 parts of krill peptide, 150 parts of oligofructose, 20 parts of purple perilla.
Embodiment 4
A kind of marine protein oligopeptide health powder, the raw material including following parts by weight: 15 parts of sea cucumber peptide, abalone peptide 15 Part, 80 parts of oyster peptide, 120 parts of oligofructose, 10 parts of sweet basil.
Embodiment 5
A kind of marine protein oligopeptide health powder, the raw material including following parts by weight: 18 parts of sea cucumber peptide, abalone peptide 12 Part, 120 parts of oyster peptide, 160 parts of inulin, 15 parts of purple perilla.
Embodiment 6
A kind of marine protein oligopeptide health powder, the raw material including following parts by weight: 12 parts of sea cucumber peptide, abalone peptide 18 Part, 90 parts of oyster peptide, 140 parts of oligofructose, 25 parts of sweet basil.
Embodiment 7
The food materials each component and its parts by weight of health powder are the same as embodiment 1-6；
The preparation of health powder the following steps are included:
(1) after being homogenized selected seafood meat, pepsin is sequentially added into homogenate and trypsase is digested, Obtain enzymolysis liquid, after enzymatic hydrolysis under the conditions of 100 DEG C enzyme deactivation 15min；Obtained enzymolysis liquid is centrifuged removal upper layer floating material And bottom sediment, retain supernatant；Obtained supernatant is centrifuged to removal precipitating again, and selecting molecular cut off is 2000Da Ultrafiltration membrane carry out ultra filtering clarifying, obtained ultrafiltrate is spray-dried, marine oligopeptide powder is prepared；
(2) the oligopeptide powder of each marine product in health preserving soup powder component is prepared respectively using step (1) method, then basis The parts by weight of each component distinguish accurate weighing each component, are crushed after mixing；
(3) smashed raw material is crossed into 80 meshes, health powder is prepared.
Embodiment 8
The food materials each component and its parts by weight of health powder are the same as embodiment 1-6；
The preparation of health powder the following steps are included:
(1) after being homogenized selected seafood meat, pepsin is sequentially added into homogenate and trypsase is digested, Obtain enzymolysis liquid, after enzymatic hydrolysis under the conditions of 100 DEG C enzyme deactivation 15min；Obtained enzymolysis liquid is centrifuged removal upper layer floating material And bottom sediment, retain supernatant；Obtained supernatant is centrifuged to removal precipitating again, and selecting molecular cut off is 1000Da Ultrafiltration membrane carry out ultra filtering clarifying, obtained ultrafiltrate is spray-dried, marine oligopeptide powder is prepared；
(2) the oligopeptide powder of each marine product in health preserving soup powder component is prepared respectively using step (1) method, then basis The parts by weight of each component distinguish accurate weighing each component, are crushed after mixing；
(3) smashed raw material is crossed into 80 meshes, health powder is prepared.
Embodiment 9
The preparation method of health powder with embodiment 7-8, edible way be for making soup product or water being added to drink, according to Primary plus water is drunk amount required for required amount or production soup product and is dispensed, and finished product is packaged to be.
Embodiment 10
The health powder of embodiment 7-8 can be prepared into the form of solid particle, and the preparation method of health preserving soup powder particles is such as Under:
(1) the health powder being prepared is added in V-Mixer and is mixed 12 minutes, 95% ethanol solution is added and glues Wetting agent is closed, continues stirring and softwood is made, pelletized with 16 mesh of oscillating granulator；
(2) particle made is entered into boiling drier, drying temperature is 50 DEG C, and pellet moisture is controlled 2% or so；It will 12 mesh sieves of the particle of drying；
(3) particle obtained is dispensed, according to production soup product, once required amounts of particles is packed, and is obtained Finished product.
Embodiment 11
The health powder of embodiment 7-8 can be prepared into the form of solid particle, and the preparation method of health preserving soup powder particles is such as Under:
(1) the health powder being prepared is added in V-Mixer and is mixed 15 minutes, 95% ethanol solution is added and glues Wetting agent is closed, continues stirring and softwood is made, pelletized with 16 mesh of oscillating granulator；
(2) particle made is entered into boiling drier, drying temperature is 40 DEG C, and pellet moisture is controlled 2% or so；It will 12 mesh sieves of the particle of drying；
(3) particle obtained is dispensed, the amounts of particles according to required for one glass when water being added to drink is packed, and is obtained To finished product.
Embodiment 12
The health powder of embodiment 7-8 can be prepared into the form of solid particle, and the preparation method of health preserving soup powder particles is such as Under:
(1) the health powder being prepared is added in V-Mixer and is mixed 10 minutes, 95% ethanol solution is added and glues Wetting agent is closed, continues stirring and softwood is made, pelletized with 16 mesh of oscillating granulator；
(2) particle made is entered into boiling drier, drying temperature is 55 DEG C, and pellet moisture is controlled 2% or so；It will 12 mesh sieves of the particle of drying；
(3) particle obtained is dispensed, is packed, is obtained according to amounts of particles required for production several person-portions of soup product To finished product.
Test example
Antifatigue effect test is carried out to albumen oligopeptide health powder provided by the invention
It gives mouse of the invention albumen oligopeptide health powder 30 days, monitors its swimming with a load attached to the body, hepatic glycogen content, blood urine Plain nitrogen content, blood lactase acid are horizontal, evaluate the antifatigue effect of albumen oligopeptide health powder.
1. experimental material and instrument
The albumen oligopeptide health powder (TYS-L, TYS-M, TYS-H) of Three doses is spare；
Positive reference substance are as follows: American ginseng buccal tablet, product batch number: 20171202, production unit: the limited public affairs of Kang Mei medicine company share Department；
Experimental subjects: Kunming mouse, male, 18-22 grams of weight, by Qingdao of Shandong province experimental animal and zoopery Center provides.
Instrument and reagent: XMARK UV-2550 microplate reader, BIORAD company, the U.S.；Life is built up in hepatic glycogen kit, Nanjing Object Graduate School of Engineering
2. experimental method
The grouping and nursing of 2.1 mouse
It tests and sets 6 groups: 1) blank group altogether, 2) control group, 3) positive controls (American Ginseng), 4) TYS-L low dose group (TYS-L), 5) TYS middle dose group (TYS-M), 6) high dose group (TYS-H).
Measurement mouse swimming time, every group 15, totally 90；
The variation of each physiological and biochemical index in vivo after measurement swimming 1h, every group 10, totally 60.
Dose design foundation: normal adult (weight 60kg) is oral by 3g/d, then middle dosage is set as 6 times of conventional amount used i.e. 300mg/kg, high and low dose are set to 900mg/kg, 100mg/kg.Therefore, TYS-L group is filled according to 0.1mg/g/d dosage Stomach, TYS-M group are 0.3mg/g/d, TYS-H 0.9mg/g/d, American Ginseng positive controls according to 0.4mg/g/d dosage stomach-filling, Control group is stomach-filling distilled water, and blank group is not handle, and administration frequency is 1 times/day, and administration time is 30 days.
2.2 mouse power exhaust swimming test
The most important performance of fatigue is exercise tolerance decline, during persistent movement, the increase of muscle oxygen amount, and blood glucose Consumption with protein can generate fatigue, and the reduction of exercise tolerance is most direct, the most objective index of reflection organism fatigue degree. We have selected the power being widely used in current evaluation substance fatigue resistance to exhaust necton model test, and mouse power exhausts trip The swimming time is longer, illustrates that the substance relieves fatigue effect and is more obvious.This swimming experiment device of mouse is easy, operating procedure letter Single, controllable factor is stronger, and experimental result more can intuitively react the exercise tolerance of body.
For 6 groups of mouse after last dose 30min, load 5% weight sheet lead in mouse tail root is placed in swimming trunk (water temperature 25 ± 1 DEG C, the depth of water is no less than 30cm) swimming records until 10s does not refloat and exhausts death standard as power after mouse sinks under water Since swimming to the death time, as mouse swimming time, the result is shown in Figure 1.
The variation of 2.3 mouse physiological and biochemical indexs is tested
Glycolytic cycle is considered as a kind of mode that biological most original obtains energy, and glycolysis is that convert glucose is third Ketone acid generates the series reaction that ATP is organism energy supply.Under anaerobic, glycolysis can be lactic acid from convert glucose, Carry out substance decomposition process.Therefore, in the case of a hard exercise, muscle oxygen amount increases so as to cause body anoxic, sugared ferment Solution reaction accelerates, and a large amount of lactic acid is generated, so that H+Concentration increases, so that body pH value is caused to decline, lactic acid/H in muscle+Concentration Increase is the main reason for causing muscular fatigue.
Amino generates NH3And CO2, the two urea synthesis in liver excreted by kidney.Under normal physiological condition, The generation and excretion of plasma wrea are in equilibrium state, and after strenuous exercise, body cannot pass through sugar, fat and protein for a long time When catabolism obtains enough energy, plasma wrea level can be obviously increased, and the content of blood urea nitrogen directly represents plasma wrea Level.Therefore blood urea nitrogen can evaluate the size of exercise load.In addition, plasma wrea level and exercise tolerance are in vivo It is significant negatively correlated, that is, blood urea nitrogen level is lower, and tolerance of exercise is better.
The failure of physical strength always occurs with the exhaustion of muscle glycogen simultaneously in long-time strenuous exercise, when strenuous exercise, machine Body blood glucose is insufficient, and while muscle glycogen consumes, hepatic glycogen can be decomposed into glucose, is converted into energy, maintains blood glucose level with full The needs of sufficient body.Therefore the high and low energy of glycogen content illustrates the degree of fatigue generation, and the content height of usual hepatic glycogen is transported The influence of fatigue resistance, hepatic glycogen content is higher in body, and tolerance of exercise is stronger, in the feelings of body strenuous exercise consumption energy Under condition, energy is provided for body in time to maintain blood glucose level, delay fatigue occurs.Conversely, if when hepatic glycogen is largely consumed, Fatigue will occur.Therefore, hepatic glycogen content height is the important symbol for measuring body movement tolerance, and measurement fatigue is One important indicator of no generation.
Mouse last dose 30min, mouse are placed in swimming trunk (25 ± 1 DEG C of water temperature, the depth of water is no less than 30cm) swimming, trip After swimming 1h, centrifuging and taking serum of taking a blood sample, with the content of lactic acid (LA), blood urea nitrogen (BUN) in Elisa method measurement serum；It takes Mouse liver, with glycogen kit measurement hepatic glycogen content.
Variance analysis is carried out to mouse LA, BUN using software systems, is indicated with mean ± standard deviation, the results are shown in Table 1；It is right Hepatic glycogen is analyzed, and as a result sees Fig. 2.
3. experimental result and discussion
The measurement of 3.1 mouse swimming times
It can be seen from figure 1 that the time that TYS-H group power exhausts swimming is 160.8min, it is 4.5 times of control group (35.8min), TYS-M (100.0min) and TYS-L (85.8min) group mouse swimming time is respectively 2.8 times and 2.4 times of control group, The dosage and being positively correlated property of mouse swimming time of TYS.Significant difference (P < is reached between each dosage group of TYS and control group 0.05), illustrate that TYS dosage group can significantly extend the swimming time of exercised rats, there is certain antifatigue effect.West American ginseng positive controls are also able to extend mouse swimming time, and there are significant difference (P < between TYS dosage group 0.05)。
The measurement of 3.2 mouse lactic acid (LA) content's index
As known from Table 1, it swims after 1h, control group mice lactic acid content is about 1294ug/L, increases by 21% than blank group, says After bright mouse swimming, internal lactic acid is caused to accumulate, fatigue generates.Compared with the control group, American Ginseng positive group can significantly lower small The intracorporal lactic acid content of mouse reaches significant difference (P < 0.05) between two groups.TYS dosage group lactic acid content has compared with control group Declined, reduces 14%-19% than control group respectively, and each dosage group and control group reach significant difference (P < 0.05), say The intracorporal lactic acid accumulation of mouse after movement can be alleviated or be removed to bright TYS, have certain antifatigue effect.
Influence of the table 1T YS to mouse LA, BUN content
Group LA(ug/L) BUN(mmol/L)
Blank group 1072.83±79.42 5.08±0.24
Control group 1294.75±135.48 5.59±0.27
American Ginseng 1022.9±69.31 4.67±0.07
TYS-L 1108.01±120.19 4.63±0.21
TYS-M 1046.20±101.99 4.70±0.21
TYS-H 1113.89±98.62 4.71±0.30
The measurement of 3.3 mouse blood urea nitrogen (BUN) contents
Table 1 reflects the situation of change of each group mouse blood urea nitrogen, and after the 1h that swims, control group mice blood urea nitrogen content increases It is added to 5.59mmol/L, improves 10% than blank group, illustrates that strenuous exercise can improve the level of blood urea nitrogen of mouse.TYS Dosage group blood urea nitrogen content compared with the control group, reduces 17%, 16% and 16%, and TYS dosage group and control group respectively Between reached significant difference (P < 0.05), illustrate that stomach-filling TYS provides energy for mouse body, slow down protein metabolism water It is flat, to reduce the content of blood urea nitrogen, the exercise tolerance of mouse is improved, there is certain anti-fatigue effect.
3.4 the measurement of mouse hepatic glycogen content
From Figure 2 it can be seen that control group mice hepatic glycogen level is 6.6mg/g, significant compared with blank group 9.3mg/g after swimming 1h It reduces (P < 0.05), the hepatic glycogen level of each dosage group of TYS is respectively 8.8mg/g, 9.8mg/g, 8.7mg/g, than compareing component Be not higher by 33.3%, 48.5% and 31.8%, difference has reached conspicuousness (P < 0.05), and American Ginseng positive controls with it is right According to there was no significant difference between group (P > 0.05).Illustrate that stomach-filling TYS may improve the energetic supersession system of mouse, reduces glycogen Former decomposition, improves the exercise tolerance ability of body, has certain anti-fatigue effect.
Above description is merely a preferred embodiment of the present invention, and is not limitation of the present invention, all in the contents of the present invention Any modification, equivalent replacement, the remodeling etc. made in range, should be included within scope of patent protection of the invention.

Claims (10)

1. a kind of marine protein oligopeptide health powder, which is characterized in that natural including peptides, dietary fiber class and seasoning class Food materials, the peptides are the oligopeptide extracted in marine product, and the peptides include sea cucumber peptide, abalone peptide, krill peptide or oyster Peptide, the dietary fiber class include that perhaps the oligofructose seasoning class includes purple perilla or sweet basil to inulin, the food materials Parts by weight composition is as follows: 70-180 parts of peptides, 120-180 parts of dietary fiber class, 10-30 parts of seasoning class.
2. a kind of marine protein oligopeptide health powder according to claim 1, which is characterized in that including following parts by weight Component: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, 60-140 parts of krill peptide, 120-180 parts of inulin, 10-30 parts of sweet basil.
3. a kind of marine protein oligopeptide health powder according to claim 1, which is characterized in that including following parts by weight Component: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, 60-140 parts of oyster peptide, 120-180 parts of inulin, 10-30 parts of purple perilla.
4. a kind of marine protein oligopeptide health powder according to claim 1, which is characterized in that including following parts by weight Component: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, 60-140 parts of krill peptide, 120-180 parts of oligofructose, purple perilla 10-30 Part.
5. a kind of marine protein oligopeptide health powder according to claim 1, which is characterized in that including following parts by weight Component: 5-20 parts of sea cucumber peptide, 5-20 parts of abalone peptide, 60-140 parts of oyster peptide, 120-180 parts of oligofructose, sweet basil 10-30 Part.
6. a kind of marine protein oligopeptide health powder according to claim 1, which is characterized in that the health powder Food materials further include B family vitamin class.
7. a kind of marine protein oligopeptide health powder according to claim 1, which is characterized in that the marine product also wraps Include fish or scallop seafood.
8. a kind of marine protein oligopeptide health powder according to claim 1-7, which is characterized in that described to support The preparation step of raw powder is as follows:
(1) seafood meat is homogenized, sequentially adds pepsin and trypsase is digested, obtains enzymolysis liquid, enzyme deactivation；It will obtain Enzymolysis liquid centrifugation retain supernatant, again centrifugation removal precipitating, and by ultrafiltration membrane carry out ultrafiltration, obtain ultrafiltrate progress Spray drying, is prepared oligopeptide powder；
(2) the oligopeptide powder for preparing each marine product in health preserving soup powder component respectively using step (1) method, then according to each group The parts by weight divided distinguish accurate weighing each component, are crushed after mixing；
(3) smashed raw material is sieved, health powder is prepared.
9. a kind of marine protein oligopeptide health powder according to claim 8, which is characterized in that the health powder system It is standby at solid particle, the preparation method of the particle the following steps are included:
(1) the health powder being prepared is added in mixing machine and is mixed, the stirring of 95% ethanol solution is added, softwood is made, make Grain；
(2) after the particle made being dried, whole grain of being sieved；
(3) particle obtained dispensed, packed, obtain finished product.
10. a kind of marine protein oligopeptide health powder according to claim 1, which is characterized in that the health powder For making soup product or directly water being added to drink.
CN201910910186.7A 2019-09-25 2019-09-25 A kind of marine protein oligopeptide health powder Pending CN110463962A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910910186.7A CN110463962A (en) 2019-09-25 2019-09-25 A kind of marine protein oligopeptide health powder

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910910186.7A CN110463962A (en) 2019-09-25 2019-09-25 A kind of marine protein oligopeptide health powder

Publications (1)

Publication Number Publication Date
CN110463962A true CN110463962A (en) 2019-11-19

Family

ID=68516785

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910910186.7A Pending CN110463962A (en) 2019-09-25 2019-09-25 A kind of marine protein oligopeptide health powder

Country Status (1)

Country Link
CN (1) CN110463962A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111184222A (en) * 2019-12-16 2020-05-22 福建省水产研究所（福建水产病害防治中心） Marine short peptide type full-nutrition formula food
CN111938062A (en) * 2020-08-19 2020-11-17 广东海洋大学深圳研究院 Oyster protein solid beverage rich in biological zinc and preparation method thereof
CN112352891A (en) * 2020-10-27 2021-02-12 安徽济生元药业有限公司 Sea sipunculus and sea cucumber polypeptide drink and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104472848A (en) * 2014-12-30 2015-04-01 广东海洋大学 Method for preparing low-value seashell oligopeptide based on simulated digestion
CN105132498A (en) * 2015-07-30 2015-12-09 北京天肽生物科技有限公司 Micromolecular sea cucumber peptide and preparation method thereof
CN106135907A (en) * 2015-04-11 2016-11-23 青岛贝尔特生物科技有限公司 A kind of alimentation composition contributing to resisting fatigue
CN106901240A (en) * 2016-12-30 2017-06-30 浙江海洋大学 A kind of prevention amyotrophic preparation method based on marine protein food of person in middle and old age
CN107495387A (en) * 2017-09-26 2017-12-22 大连大学 A kind of ocean polypeptide spy's dietary seafood as sports tonica
CN108887549A (en) * 2018-06-29 2018-11-27 南京医科大学 Anti-fatigue solid beverage, preparation method and its major ingredient composition

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104472848A (en) * 2014-12-30 2015-04-01 广东海洋大学 Method for preparing low-value seashell oligopeptide based on simulated digestion
CN106135907A (en) * 2015-04-11 2016-11-23 青岛贝尔特生物科技有限公司 A kind of alimentation composition contributing to resisting fatigue
CN105132498A (en) * 2015-07-30 2015-12-09 北京天肽生物科技有限公司 Micromolecular sea cucumber peptide and preparation method thereof
CN106901240A (en) * 2016-12-30 2017-06-30 浙江海洋大学 A kind of prevention amyotrophic preparation method based on marine protein food of person in middle and old age
CN107495387A (en) * 2017-09-26 2017-12-22 大连大学 A kind of ocean polypeptide spy's dietary seafood as sports tonica
CN108887549A (en) * 2018-06-29 2018-11-27 南京医科大学 Anti-fatigue solid beverage, preparation method and its major ingredient composition

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张颖等: "动物源性抗疲劳肽研究与功能食品开发进展", 《农产品加工》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111184222A (en) * 2019-12-16 2020-05-22 福建省水产研究所（福建水产病害防治中心） Marine short peptide type full-nutrition formula food
CN111938062A (en) * 2020-08-19 2020-11-17 广东海洋大学深圳研究院 Oyster protein solid beverage rich in biological zinc and preparation method thereof
CN112352891A (en) * 2020-10-27 2021-02-12 安徽济生元药业有限公司 Sea sipunculus and sea cucumber polypeptide drink and preparation method thereof
CN112352891B (en) * 2020-10-27 2023-09-12 安徽济生元药业有限公司 Sipunculus nudus and sea cucumber polypeptide drink and preparation method thereof

Similar Documents

Publication Publication Date Title
CN102090692B (en) Oyster polypeptide anti-fatigue beverage and preparation method thereof
JP5433003B2 (en) Yeast nutritional food and method for producing the same
CN110463962A (en) A kind of marine protein oligopeptide health powder
CN108272073A (en) A kind of weight losing meal-replacing powder and preparation method thereof
CN102429066B (en) Physical strength-enhancing anti-fatigue compound Maca coffee product
CN101406309B (en) Method for preparing concentrated peptides of white fish and uses thereof
CN102524872B (en) Ginseng peptide amino acid drink
CN102406186A (en) Organic selenium chromium whole egg powder and production method thereof
CN108056426A (en) A kind of preparation method of food/drink based on potato dietary fiber
CN111802653A (en) Tuna oligopeptide powder composition and preparation method and application thereof
CN107183306A (en) A kind of soybean protein powder producing method with intestinal regulatory function
CN113729139A (en) Solid beverage and production process thereof
CN103392799B (en) Castanea mollissima peptide lactic acid bacteria beverage and preparation method thereof
CN109170916A (en) One kind keeps fit and healthy food compositions and preparation method thereof
CN103535576A (en) Meal replacement powder containing pollen
CN106306921A (en) Method for preparing chitosan oligosaccharide functional beverage with Maca and Cordyceps sinensis and functional beverage made thereby
CN100363387C (en) Mumia protein and its production process
CN108308499A (en) Quinoa oligopeptide protein solid beverage and preparation method
CN105688007A (en) Preparation method of ferment capable of maintaining and repairing liver function
CN105123948B (en) A kind of U.S. face skin care Goat milk powder improving immunity and anti-aging
KR101317935B1 (en) A culture medium for making the complex vegitables watery kimchi and method for producing thereof using the same
CN114097970A (en) Marine active peptide energy beverage and preparation method thereof
CN101700068B (en) Preparation method of high calcium beverage capable of promoting skeleton growth of children
CN107712875A (en) Black quinoa weight losing meal-replacing
CN106615137A (en) Protein peptide solid beverage and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information

Inventor after: Li Yujin

Inventor after: Yu Haiqiao

Inventor after: Zhao Yalin

Inventor after: Cao Minjie

Inventor before: Yu Haiqiao

Inventor before: Zhao Yalin

CB03 Change of inventor or designer information
RJ01 Rejection of invention patent application after publication

Application publication date: 20191119

RJ01 Rejection of invention patent application after publication