KR100526164B1 - Composition for enhancing exercise performance - Google Patents

Composition for enhancing exercise performance Download PDF

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KR100526164B1
KR100526164B1 KR1020050030480A KR20050030480A KR100526164B1 KR 100526164 B1 KR100526164 B1 KR 100526164B1 KR 1020050030480 A KR1020050030480 A KR 1020050030480A KR 20050030480 A KR20050030480 A KR 20050030480A KR 100526164 B1 KR100526164 B1 KR 100526164B1
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weight
composition
training
group
present
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한대석
박태선
백일영
김인호
김영언
성기승
이창호
오세욱
정경아
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한국식품연구원
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives

Abstract

본 발명은 운동수행능력 증강용 조성물에 관한 것이다. 보다 상세하게는, 본 발명은 스쿠알렌 및 식물 추출물류를 포함하는 운동수행능력 증강용 조성물에 관한 것이다. 상기 본 발명에 따른 조성물은 장기간 섭취시 운동수행시간을 증진시키며 혈중 피로물질의 축적을 억제하는 효과가 있어서 운동선수의 체력 및 운동능력 증진에 유용하다.The present invention relates to a composition for enhancing athletic performance. More specifically, the present invention relates to a composition for enhancing athletic performance including squalene and plant extracts. The composition according to the present invention is useful for improving physical fitness and athletic ability of the athlete because it has an effect of enhancing exercise performance time and prohibiting accumulation of blood fatigue substances during long-term ingestion.

Description

운동수행능력 증강용 조성물{Composition for enhancing exercise performance}Composition for enhancing exercise performance

본 발명은 운동수행능력 증강용 조성물에 관한 것이다. 보다 상세하게는, 본 발명은 스쿠알렌 및 식물 추출물류를 포함하는 운동수행능력 증강용 조성물에 관한 것으로서, 장기간 섭취시 운동수행능력을 증진시키며 혈중 피로물질의 축적을 억제하는 효과가 있어서 운동선수의 체력 및 운동능력 증진에 유용한 조성물에 관한 것이다. The present invention relates to a composition for enhancing athletic performance. More specifically, the present invention relates to a composition for enhancing athletic performance comprising squalene and plant extracts, and has an effect of improving athletic performance during long-term intake and inhibiting the accumulation of blood fatigue substances in athletes. And compositions useful for enhancing athletic performance.

운동선수는 항상 새로운 기록을 세우기 위하여 자신이 가진 체력과 기술을 최대로 발휘하고자 노력한다. 이를 위해서 운동 종목에 따른 과학적인 훈련, 식이요법, 기술·장비의 개선 및 운동능력 향상 보조제(ergogenic aids) 등이 사용되고 있다. 이 중 운동능력 향상 보조제는 운동수행능력의 향상 뿐 만 아니라, 신체 활동 시 체내에 축적되어 피로를 발생시키는 피로요소의 제거에도 효과가 있기 때문에 운동선수들 뿐 만 아니라 일반인들에게도 흔히 사용되고 있다.Athletes always strive to their fullest strength and skills to set new records. For this purpose, scientific training, diet, technology and equipment improvement, and ergogenic aids are used. Among them, exercise aids are used not only for athletes, but also for the elimination of fatigue factors that accumulate in the body and cause fatigue.

운동능력 향상을 위한 기능성 보조제와 관련된 연구는 동서양을 막론하고 활발하게 수행되고 있다. 한편, 스테로이드, 카페인, 탄산수소나트륨 및 구연산나트륨 등과 같은 화합물을 포함하는 보조제는 일정량 이상 복용하면 운동 수행능력을 현저히 증가시킬 수 있으나 상기와 같은 약물은 치명적인 부작용을 수반하여 궁극적으로 건강을 해치게 되는 위험이 있다. 따라서, 최근에는 식물 추출물과 같이 안전성이 보장된 천연물을 이용하여 기능성 보조제를 개발하고자 하는 연구가 활발히 진행되고 있다.Research on functional supplements for improving athletic performance is being actively conducted in both East and West. On the other hand, supplements containing compounds such as steroids, caffeine, sodium bicarbonate and sodium citrate can significantly increase exercise performance when taken in a certain amount, but such drugs may have fatal side effects and ultimately damage health. There is this. Therefore, in recent years, researches are being actively conducted to develop functional supplements by using natural products having a guaranteed safety such as plant extracts.

이에 본 발명자들은 운동수행능력을 효과적으로 증강시키면서도 부작용이 없는 보조제에 대해 연구하던 중, 식품용 첨가물류와 식물 추출물로 이루어진 혼합 조성물을 제조하고 상기 혼합 조성물의 섭취에 따른 운동수행능력을 분석한 결과 상기 혼합 조성물을 장기간 섭취하면 운동수행능력이 증가되고 혈중 피로요소의 축적이 억제됨을 확인함으로써 본 발명을 완성하였다.The present inventors while studying the supplements without side effects while effectively enhancing the exercise performance, preparing a mixed composition consisting of food additives and plant extracts and analyzed the exercise performance according to the intake of the mixed composition When the mixed composition is ingested for a long time, the present invention was completed by confirming that exercise performance was increased and blood accumulation of accumulation of fatigue elements was suppressed.

따라서, 본 발명의 목적은 스쿠알렌 및 식물 추출물류를 포함하는 운동수행능력 증강용 조성물을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a composition for enhancing exercise performance including squalene and plant extracts.

상기와 같은 목적을 달성하기 위하여, 본 발명은 스쿠알렌 및 식물 추출물류 를 포함하는 운동수행능력 증강용 조성물을 제공한다.In order to achieve the above object, the present invention provides a composition for enhancing exercise performance, including squalene and plant extracts.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명에 따른 조성물은 스쿠알렌 25∼35중량%, 삼백초 수추출물 20∼25중량, 오가피 수추출물 20∼25중량% 및 동충하초 수추출물 20∼25중량%로 포함하는 것을 특징으로 한다. 보다 바람직하게는, 본 발명에 따른 조성물은 스쿠알렌 31중량%, 삼백초 수추출물 23중량%, 오가피 수추출물 23중량% 및 동충하초 수추출물 23중량%를 포함할 수 있다.The composition according to the present invention is characterized in that it comprises 25 to 35% by weight of squalene, 20 to 25% by weight of 300 seconds water extract, 20 to 25% by weight of Ogapi water extract and 20 to 25% by weight of Cordyceps sinensis extract. More preferably, the composition according to the present invention may comprise 31% by weight of squalene, 23% by weight of 300 seconds water extract, 23% by weight of Ogapi water extract and 23% by weight of Cordyceps sinensis extract.

상기에서 삼백초, 오가피 및 동충하초의 수추출물은 공지의 방법에 따라 제조하여 사용할 수 있다. 또는 상기 수추출물들은 시판되는 것을 구입하여 사용할 수 있다. 예를 들면, 삼백초, 오가피 및 동충하초 각각에 음용수를 중량비로 2배∼10배의 양으로 가하고 80∼100℃에서 20∼60분간 가열하여 제조하거나 (주)다송식품원료(한국)사로부터 구입하여 사용할 수 있다.The water extract of three hundred s, Ogapi and Cordyceps sinensis can be prepared and used according to a known method. Alternatively, the water extracts may be purchased and used commercially. For example, drinking water was added to the amount of 2 to 10 times in weight ratio to each of the three hundred seconds, Ogapi and Cordyceps sinensis and heated for 20 to 60 minutes at 80-100 ° C, or purchased from Dasong Food Raw Materials Co., Ltd. Can be used.

상기에서 스쿠알렌은 6개의 이중결합을 가진 고도불포화 탄화수소로서 일반적으로 심해상어의 간유에 추출하여 정제함으로서 제조된다. 스쿠알렌의 생리활성으로는 산소공급작용 및 살균작용 등이 있다. 특히, 스쿠알렌은 인체의 물 분자에서 수소를 결합하고 산소를 방출함으로써 방출된 산소가 체내의 세포에 산소를 공급하여 세포를 활성화시킨다고 알려져 있다. In the above, squalene is a polyunsaturated hydrocarbon having six double bonds, and is generally prepared by extracting and refining it into liver oil of a deep sea shark. The physiological activities of squalene include oxygen supply and sterilization. In particular, squalene is known to bind oxygen and release oxygen from the water molecules of the human body, thereby activating the cells by releasing oxygen to supply the cells in the body.

삼백초는 오래살이풀로서 그 약리작용이 매우 다양하다. 즉, 변비, 당뇨병, 간장병, 암, 고혈압, 심장병, 부인병 및 신장병과 같은 성인병의 예방과 치료에 주목할 만한 효과가 있는 것으로 알려져 있다. Three hundred seconds is an old grass and its pharmacological action is very diverse. That is, it is known to have a remarkable effect on the prevention and treatment of adult diseases such as constipation, diabetes, liver disease, cancer, hypertension, heart disease, gynecological diseases and kidney disease.

오가피는 두릅나무과 낙엽관목인 가시오갈피의 뿌리와 나무껍질을 건조시킨 것을 말하며 양위, 관절염, 요통, 퇴행성 관절염 증후군, 수종, 각기, 타박상 및 종창 등에 처방하는 것으로 알려져 있다. Ogapi refers to dried roots and bark of the bark of deciduous tree, arthropod and deciduous tree, and is known to be prescribed for stomach, arthritis, back pain, degenerative arthritis syndrome, species, each, bruise and swelling.

동충하초는 자낭균류에 속하는 소형 버섯류로서 대부분 곤충에 기생하여 숙주가 되는 곤충의 시체에 자실체를 낸다. 상기 동충하초의 생리활성으로는 기관지를 정화하고 혈관 내에 부착된 불순물을 제거하며 심장의 수축력을 강화하는 것으로 알려져 있다. 또한, 세포의 활성화와 회복, 면역력 강화, 혈당치의 정상화, 빈혈 및 비만에 효과가 있는 것으로 알려져 있다.Cordyceps sinensis is a small mushroom belonging to the Aspergillus fungi, and most of them are parasitic to insects and give fruit bodies to the host of insect host. The physiological activity of Cordyceps sinensis is known to purify the bronchus, remove impurities attached to blood vessels, and strengthen the contractile force of the heart. It is also known to be effective in activating and restoring cells, strengthening immunity, normalizing blood glucose levels, anemia and obesity.

또한, 본 발명에 따른 운동수행능력 증강용 조성물은 콜라넛 분말 또는 추출물(Cola nut extract), 타우린, 포스파티딜콜린, 글루타민, L-아르기닌 및 L-카르니틴을 추가로 포함할 수 있다. 바람직하게는, 스쿠알렌 및 식물 추출물류로 이루어진 상기 혼합 조성물 100중량부에 대하여 콜라넛 분말 또는 추출물 10∼20중량부, 타우린 10∼20중량부, 포스파티딜콜린 1∼5중량부, 글루타민 5∼10중량부, L-아르기닌 5∼10중량부 및 L-카르니틴 5∼10중량부를 추가로 포함할 수 있다. 바람직하게는, 콜라넛 분말 또는 추출물 15.6중량부, 타우린 15.6중량부, 포스파티딜콜린 3.1중량부, 글루타민 6.1중량부, L-아르기닌 6.1중량부 및 L-카르니틴 7.8중량부를 추가로 포함할 수 있다.In addition, the composition for enhancing exercise performance according to the present invention may further include a collagen powder or extract (Cola nut extract), taurine, phosphatidylcholine, glutamine, L-arginine and L-carnitine. Preferably, 10 to 20 parts by weight of the collagen powder or extract, 10 to 20 parts by weight of taurine, 1 to 5 parts by weight of phosphatidylcholine, and 5 to 10 parts by weight of glutamine, based on 100 parts by weight of the mixed composition consisting of squalene and plant extracts. 5 to 10 parts by weight of L-arginine and 5 to 10 parts by weight of L-carnitine may be further included. Preferably, 15.6 parts by weight of the collagen powder or extract, 15.6 parts by weight of taurine, 3.1 parts by weight of phosphatidylcholine, 6.1 parts by weight of glutamine, 6.1 parts by weight of L-arginine and 7.8 parts by weight of L-carnitine may be further included.

상기 타우린, 글루타민, L-아르기닌 및 L-카르니틴과 같은 아미노산류는 운동 후 근육의 피로 회복을 도우며 직접 에너지원으로 사용되기도 한다.Amino acids such as taurine, glutamine, L-arginine and L-carnitine may be used as direct energy sources to help muscle fatigue after exercise.

상기 포스파티딜콜린은 지방, 인 및 질소의 화합물로서 난황·콩기름·간·뇌 등에 다량 존재한다. 세포막 구성의 중요한 성분 중 하나이며, 피로회복에 효과적으로 알려져 있다.The phosphatidylcholine is present in egg yolk, soybean oil, liver, brain and the like as a compound of fat, phosphorus and nitrogen. It is one of the important components of cell membrane composition and is known for fatigue recovery.

상기 콜라넛은 벽오동과(Sterculiaceae)에 속하며 콜라 아쿠미나타(Cola acuminata)와 콜라 니티다(C. nitida)의 카페인이 들어있는 열매를 말한다. 아프리카 열대지역이 원산지이며 알코올 성분이 없는 음료와 의약품의 원료로 사용되고 있고 약물중독, 숙취 및 설사를 치료하는 생약으로 쓰이고 있다.The colanut belongs to the cactus (Sterculiaceae) and refers to the fruit containing the caffeine of Cola acuminata ( Cla nitida ) and C. nitida ( C. nitida ). Native to tropical Africa, it is used as a raw material for non-alcoholic beverages and medicines and as a herbal medicine to treat drug addiction, hangover and diarrhea.

본 발명의 조성물에 포함되는 콜라넛은 추출물의 형태이거나 분말 형태일 수 있다. 콜라넛 추출물 및 콜라넛 분말은 시판되고 있는 것을 삼정향료)사로부터 구입하여 사용할 수 있다. The collagen contained in the composition of the present invention may be in the form of an extract or powder form. The collagen extract and the collagen powder are commercially available from Samjunghyang Co., Ltd. can be used.

본 발명의 일 실시예에서는 스쿠알렌 9.1g, 삼백초 수추출물 6.8g, 오가피 수추출물 6.8g, 동충하초 수추출물 6.8g, 콜라넛 추출물 4.6g, 타우린 4.6g, 포스파티딜콜린 0.9g, 글루타민 1.8g, L-아르기닌 1.8g 및 L-카르니틴 2.3g을 혼합하여 본 발명에 따른 조성물을 제조하였다.In one embodiment of the present invention, squalene 9.1g, trichophytium extract 6.8g, Ogapi extract 6.8g, Cordyceps sinensis extract 6.8g, colanut extract 4.6g, taurine 4.6g, phosphatidylcholine 0.9g, glutamine 1.8g, L-arginine 1.8 g and 2.3 g L-carnitine were mixed to prepare a composition according to the invention.

본 발명자들은 본 발명에 따른 조성물의 섭취에 따른 운동수행능력 효과를 측정하기 위하여, 본 발명의 조성물을 랫트에 장기간 섭취시킨 후 강제 수영을 수행하도록 하여 최대수영능력을 분석하였다(실험예 1 참조).The present inventors analyzed the maximum swimming capacity by performing a forced swimming after ingesting the composition of the present invention in rats for a long time in order to measure the effect of exercise performance according to the intake of the composition according to the present invention (see Experimental Example 1) .

그 결과, 본 발명의 조성물을 섭취시킨 랫트의 경우 대조군에 비해 운동수행시간이 유의적으로 증가된 것으로 나타났다(표 3 참조).As a result, the rats ingested the composition of the present invention was found to significantly increase the exercise performance time compared to the control group (see Table 3).

또한, 본 발명자들은 본 발명의 조성물의 섭취에 따른 운동 후 혈액과 근육 내 피로요소의 변화를 조사하였다(실험예 2 참조). 혈중 피로요소로는 혈중 포도당, 유리 지방산, 중성지방, 젖산, 무기 인산, 암모니아 농도 및 크레아틴 키나제 활성을 측정하였다. 근육 내 피로요소로는 글리코겐 농도, 젖산 디하이드로게나제 활성 및 구연산 합성효소 활성을 측정하였다. 실험 결과, 본 발명의 조성물을 섭취하는 경우 혈중 및 근육 내 피로요소는 본 발명의 조성물을 섭취하지 않은 대조군에 비해 유의적인 차이가 없거나 오히려 더 낮게 나타났다.In addition, the present inventors investigated changes in blood and muscle fatigue factors after exercise according to the ingestion of the composition of the present invention (see Experimental Example 2). Blood fatigue factors were measured by blood glucose, free fatty acids, triglycerides, lactic acid, inorganic phosphate, ammonia concentration and creatine kinase activity. As muscle fatigue factors, glycogen concentration, lactate dehydrogenase activity, and citric acid synthase activity were measured. As a result of the experiment, the blood and intramuscular fatigue factor of the composition of the present invention showed no significant difference or lower than that of the control group not receiving the composition of the present invention.

이로부터 본 발명자들은 본 발명의 조성물을 섭취함으로써 운동수행에 따른 피로요소의 축적이 억제되어 운동수행시간을 연장되는 효과가 있음을 알 수 있었다.From this, the inventors have found that by ingesting the composition of the present invention, the accumulation of fatigue factors due to exercise performance is suppressed, thereby extending the exercise performance time.

상기에서 운동수행능력을 증진시키는 효과가 있음이 입증된 본 발명에 따른 조성물의 바람직한 섭취량은 특별히 한정되지는 않으나, 1인 1회 섭취시 4.0∼6.0g/체중 kg, 바람직하게는 약 4.5g/체중 kg이다. Preferred intake of the composition according to the present invention has been shown to have the effect of enhancing the exercise performance in the above is not particularly limited, but intake once per person 4.0 to 6.0 g / kg body weight, preferably about 4.5 g / Weight kg.

또한, 본 발명에 따른 조성물은 차, 쥬스 및 드링크의 형태로 제조하여 음용하도록 하거나, 과립화, 캡슐화 및 분말화하여 섭취할 수 있다. 또한, 본 발명의 조성물은 운동수행능력을 증진시키는 보조제로서 알려진 공지의 성분과 함께 혼합 조성물의 형태로 제조할 수 있다. In addition, the composition according to the present invention can be prepared in the form of tea, juice and drink to drink, or ingested by granulation, encapsulation and powdered. In addition, the composition of the present invention can be prepared in the form of a mixed composition with a known component known as an adjuvant to enhance athletic performance.

바람직하게는, 본 발명의 조성물은 복용이 용이한 음료의 형태로 제공될 수 있다. 본 발명에 따른 운동수행능력 증강용 음료는 본 발명의 운동수행능력 증진용 조성물을 20∼25%(w/v), 보다 바람직하게는 24%(w/v)를 함유할 수 있다. 음료 조성물에는 통상적으로 사용되는 성분, 예컨대 감미료, 향료, 구연산, 탄산수소나트륨, 식염 및 음용수수 등을 적합한 양으로 첨가하여 제조될 수 있다. 상기 감미료로는 올리고당, 과당, 고과당, 포도당, 덱스트린 및 프락토올리고당을 사용할 수 있다. 또한, 음용자의 기호에 따라 미량의 탄산가스가 주입되어 제조될 수도 있다. 상기 구연산 및 고과당의 경우에는 음료의 pH가 약 3.2가 되도록 첨가할 수 있다. 보다 바람직하게는, 본 발명의 음료 조성물은 상기의 스쿠알렌 및 식물 추출물류가 혼합된 조성물 20∼25%(w/v), 구연산 0.1∼0.2%(w/v), 고과당 5∼15%(w/v), 향료 0.05∼0.1%(w/v) 및 잔여량의 음용수가 포함된 것일 수 있다.Preferably, the composition of the present invention may be provided in the form of a drink which is easy to take. The beverage for enhancing exercise performance according to the present invention may contain 20-25% (w / v), more preferably 24% (w / v), of the composition for improving athletic performance of the present invention. The beverage composition may be prepared by adding a conventionally used ingredients such as sweeteners, flavorings, citric acid, sodium bicarbonate, saline and drinking water in an appropriate amount. Oligosaccharides, fructose, high fructose, glucose, dextrin and fructooligosaccharides may be used as the sweetener. In addition, a small amount of carbon dioxide may be injected and prepared according to the preference of the drinker. In the case of citric acid and high fructose, the pH of the beverage may be added to about 3.2. More preferably, the beverage composition of the present invention is 20-25% (w / v) of the composition in which the squalene and plant extracts are mixed, 0.1-0.2% (w / v) citric acid, 5-15% (high fructose) w / v), fragrance 0.05-0.1% (w / v) and residual amount of drinking water may be included.

본 발명의 일 실시예에서는 본 발명의 운동수행능력 증강용 조성물 28.8g, 구연산 0.16g, 고과당 12g, 향료 0.1g을 혼합하고 여기에 음용수를 첨가하여 전체 부피가 120ml가 되도록 한 다음 파우치에 넣어 밀봉한 다음 100℃에서 30분간 가열살균한 다음 냉각하여 음료 조성물을 제조하였다(실시예 2 참조).In one embodiment of the present invention, the composition for enhancing exercise performance of the present invention 28.8g, citric acid 0.16g, high fructose 12g, 0.1g fragrance is mixed and the drinking water is added so that the total volume is 120ml and then put in a pouch After sealing, the solution was heat sterilized at 100 ° C. for 30 minutes and then cooled to prepare a beverage composition (see Example 2).

본 발명에 따른 운동수행능력 증강용 조성물은 운동선수의 체력 및 운동능력 증진에 유용한 운동능력 향상 보조제로 사용되거나 일반인의 경우 피로회복을 위한 보조제로서 사용될 수 있다.The composition for enhancing athletic performance according to the present invention may be used as an athletic improvement aid useful for improving physical strength and athletic performance of an athlete or as an auxiliary for fatigue recovery in the general public.

이하, 본 발명을 실시예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by way of examples.

단, 하기의 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기의 실시예에 한정되는 것은 아니다.However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

<실시예 1><Example 1>

본 발명에 따른 운동수행능력 증강용 조성물의 제조Preparation of composition for enhancing athletic performance according to the present invention

본 발명에 따른 운동수행능력 증강용 조성물은 스쿠알렌 9.1g(31중량%), 삼백초 수추출물 6.8g(23중량%), 오가피의 수추출물 6.8g(23중량%) 및 동충하초의 수추출물 6.8g(23중량%)을 혼합한 다음 상기 혼합물 100중량부에 대하여 콜라넛 추출물 15.6중량부(4.6g), 타우린 15.6중량부(4.6g), 포스파티딜콜린 3.1중량부(0.9g), 글루타민 6.1중량부(1.8), L-아르기닌 6.1중량부(1.8g) 및 L-카르니틴 7.8중량부(2.3g)을 추가로 혼합하여 제조하였다. 상기 삼백초, 오가피 및 동충하초 수추출물은 (주)다송식품원료(한국)에서 구입하여 사용하였다.Composition for enhancing exercise performance according to the present invention is 9.1g (31% by weight) squalene, water extract 6.8g (23% by weight), 6.8g (23% by weight) of Ogapi water extract 6.8g (Cordyceps sinensis) 23% by weight), and then 15.6 parts (4.6 g) of collagen extract, 15.6 parts (4.6 g) of taurine, 3.1 parts (0.9 g) of phosphatidylcholine, and 6.1 parts of glutamine (1.8) based on 100 parts by weight of the mixture. ), 6.1 parts by weight of L-arginine (1.8 g) and 7.8 parts by weight (2.3 g) of L-carnitine were further mixed. The three hundred, Ogapi and Cordyceps sinensis extract was purchased from Dasong Food Raw Materials (Korea).

<실험예 1>Experimental Example 1

본 발명에 따른 혼합 조성물의 운동수행능력 증강 효과 확인Confirmation of the exercise performance enhancing effect of the mixed composition according to the present invention

상기 실시예 1에서 제조한 본 발명에 따른 혼합 조성물의 운동수행능력 증강 효과를 조사하였다. 실험 결과는 SPSS/PC 9.0 프로그램을 이용하여 평균±표준편차로 나타내었으며 그룹간의 유의적인 통계차를 분석하기 위하여 p<0.05의 유의수준에서 일원분산분석(one-way ANOVA test)을 실시한 다음 던칸 다중범위 검정(Duncan's multiple range test)를 이용하여 사후검증을 하였다.The exercise performance enhancing effect of the mixed composition according to the present invention prepared in Example 1 was investigated. The experimental results were expressed as mean ± standard deviation using SPSS / PC 9.0 program. To analyze the statistical difference between groups, one-way ANOVA test was performed at the significance level of p <0.05. Post-test was performed using Duncan's multiple range test.

<1-1> 실험 동물의 사육<1-1> Breeding of Experimental Animals

5주령의 수컷 SD 랫트(Sprague-Dawley)를 1주일간 예비사육한 후 20마리씩 무작위로 세 그룹으로 나누었다. 즉, 비훈련 대조군(Sedentary control group), 훈련 대조군(exercised control group) 및 훈련 실험군(exercised formula B group)으로 나누고 상기 비훈련 대조군과 훈련 대조군에는 일반적인 실험용 식이 조성물인 AIN-93(중앙실험동물, 안산, 한국)을 자유롭게 섭취하도록 하였으며 훈련 실험군에는 상기 AIN-93에 상기 실시예 1에서 제조한 조성물을 첨가한 식이를 4주간 섭취하도록 하였다(표 1). 상기 훈련 실험군에 공급하는 식이의 총 중량은 대두유와 전분의 함량을 조절하여 다른 그룹과 동일하게 되도록 하였다. 모든 그룹에 대해 식수는 자유롭게 섭취하도록 하였다. 또한, 상기 세 그룹 중 비훈련 대조군을 제외하고 훈련 대조군과 훈련 실험군에 대해 주당 2회의 수영 적응 훈련을 4주간 실시하였다. 수영 적응 훈련 방법은 수조(크기 90×45×45cm, 물 높이 50cm, 물 온도 33∼35℃)를 이용하여 1주차에는 10분, 2주차에는 15분, 3주차에는 20분 및 4주차에는 25분간 수영 훈련을 실시하는 점증운동부하법을 사용하였다. 4주간의 수영 훈련과 식이 급여가 완료된 후 각 그룹을 탈진시까지 강제 수영을 시켰다. 동물 사육실의 환경은 온도 23±1℃, 습도 50±5% 및 12시간의 명암주기를 유지하였다.Five-week-old male SD rats (Sprague-Dawley) were preliminarily bred for one week and 20 animals were randomly divided into three groups. That is, it is divided into a non-trained control group (Sedentary control group), a training control group (exercised control group) and a training experiment group (exercised formula B group), and the non-training control group and a training control group AIN-93 (Central Experimental Animal, Ansan, Korea) was freely ingested and the training group was ingested for 4 weeks the diet added to the composition prepared in Example 1 to the AIN-93 (Table 1). The total weight of the diet fed to the training group was adjusted to the same as other groups by adjusting the content of soybean oil and starch. Drinking water was freely consumed for all groups. In addition, except for the non-trained control group among the three groups, the swimming control training group and the training experiment group were conducted twice a week for adaptive training for 4 weeks. Training method for swimming adaptation is 10 minutes in week 1, 15 minutes in week 2, 20 minutes in week 3, 25 minutes in week 4 using a water tank (size 90 × 45 × 45 cm, water height 50 cm, water temperature 33-35 ° C). Incremental exercise load was used to perform a minute swim training. After four weeks of swimming training and dietary benefits were completed, each group was forced to swim until exhaustion. Animal environment was maintained at a temperature of 23 ± 1 ° C., a humidity of 50 ± 5%, and a 12-hour light and dark cycle.

실험 식이의 조성Composition of Experimental Diet 성분ingredient 그룹별 첨가량(g/kg)Addition amount per group (g / kg) 비훈련 대조군Untrained control group 훈련 대조군Training control 훈련 실험군Training experiment group 옥수수 전분Corn starch 367.486367.486 367.486367.486 361.006361.006 덱스트린화한 옥수수전분Dextrinized Corn Starch 132132 132132 132132 수크로오스Sucrose 100100 100100 100100 카제인Casein 200200 200200 200200 L-시스틴L-cystine 33 33 33 대두유Soybean oil 100100 100100 60.8860.88 미네랄 혼합물Mineral mixtures 3535 3535 3535 비타민 혼합물Vitamin mixtures 1010 1010 1010 셀룰로오스 분말Cellulose powder 5050 5050 5050 주석산 콜린(choline bitartate)Choline bitartate 2.52.5 2.52.5 2.52.5 tert-부틸하이드로퀴논tert-butylhydroquinone 0.0140.014 0.0140.014 0.0140.014 실시예1의 조성물Composition of Example 1 -- -- 45.645.6 총량Total amount 10001000 10001000 10001000

상기에서 첨가량은 사료 제조시 사료 1 Kg당 원료 첨가량(g) 임.In the above, the addition amount is the amount of raw material addition (g) per kilogram of feed at the time of manufacture of the feed.

<1-2> 실험동물의 체중변화 및 식이효율 측정<1-2> Body weight change and dietary efficiency of experimental animals

상기 실험예 <1-1>의 실험동물의 4주간의 체중 변화 및 식이효율을 측정하였다. 체중은 주당 1회씩 측정하였으며, 식이효율은 하기식을 이용하여 측정하였다.The weight change and dietary efficiency of the experimental animals of Experimental Example <1-1> were measured for 4 weeks. Body weight was measured once a week, dietary efficiency was measured using the following equation.

식이효율= 실험기간 동안 획득한 체중/실험기간 동안 섭취한 식품의 양Dietary efficiency = weight gained during the experiment / amount of food consumed during the experiment

실험 결과, 실험 시작시 및 실험 종료시의 체중은 모든 그룹 간에 유의적인 차이를 보이지 않았으며 식이효율도 유의적인 차이를 나타내지 않았다(표 2).As a result of the experiment, body weight at the beginning of the experiment and at the end of the experiment did not show a significant difference among all groups, and there was no significant difference in dietary efficiency (Table 2).

실험동물의 체중변화 및 식이효율(평균±표준편차)Body weight change and dietary efficiency (mean ± standard deviation) of experimental animals 그룹group 체중(g)Weight (g) 획득한 체중(g/4주)Body weight gained (g / 4 weeks) 식이효율Dietary efficiency 실험 시작시At the start of the experiment 실험 종료시At the end of the experiment 비훈련 대조군Untrained control group 198.51±8.71198.51 ± 8.71 377.61±24.98377.61 ± 24.98 179.11±23.51179.11 ± 23.51 0.28±0.030.28 ± 0.03 훈련 대조군Training control 196.30±6.37196.30 ± 6.37 376.21±25.96376.21 ± 25.96 179.93±25.08179.93 ± 25.08 0.26±0.080.26 ± 0.08 훈련 실험군Training experiment group 197.20±8.24197.20 ± 8.24 365.87±27.54365.87 ± 27.54 168.69±24.98168.69 ± 24.98 0.25±0.030.25 ± 0.03

<1-3> 최대수영능력의 측정<1-3> Measurement of maximum swimming capacity

최대수영능력은 상기 실험예 <1-1>에서 각 그룹이 탈진할 때까지 강제 수영시킨 랫트가 바닥으로 가라앉아 10초 이내에 다시 수면으로 떠오르지 못하는 때까지의 시간을 기록함으로써 측정하였다.Maximum swimming capacity was measured by recording the time until the rats forced to swim until each group exhausted in the Experimental Example <1-1> to sink to the bottom and do not rise back to the surface within 10 seconds.

실험 결과, 비훈련 대조군과 훈련 대조군간에는 유의적인 차이를 보이지 않았다. 반면, 훈련 대조군에 비해 훈련 실험군의 수영시간은 유의적으로 증가한 것으로 나타났다(표 3). 따라서, 본 발명에 따른 조성물의 섭취가 운동수행시간을 증강시킬 수 있음을 알 수 있었다.As a result of the experiment, there was no significant difference between the non-training control group and the training control group. On the other hand, the swimming time was significantly increased in the training group compared to the training control group (Table 3). Therefore, it was found that the intake of the composition according to the present invention can enhance exercise performance time.

실험동물의 수영시간(평균±표준편차)Swimming time of animals (mean ± standard deviation) 그룹group 수영시간(분)Swimming time (minutes) 비훈련 대조군Untrained control group 154.29±19.21154.29 ± 19.21 훈련 대조군Training control 192.71±48.73192.71 ± 48.73 훈련 실험군Training experiment group 273.71±76.31* 273.71 ± 76.31 *

*: 훈련 대조군과 비교시 유의적인 차이가 있음(p<0.05)*: Significant difference compared to the training control group (p <0.05)

<실험예 2>Experimental Example 2

본 발명에 따른 혼합 조성물의 피로회복능력의 증강 효과Strengthening effect of fatigue recovery ability of the mixed composition according to the present invention

상기 실험예 <1-1>의 탈진시까지 강제 수영시킨 각 그룹의 랫트의 심장으로부터 혈액을 채혈하고 EDTA를 첨가하여 응고를 방지한 후 3000rpm에서 10분동안 원심분리하여 혈장을 취득한 다음 이를 분석용 시료로 사용하였으며 분석시까지 -70℃에서 보관하였다.Blood was collected from the heart of each group of rats forced to swim until exhaustion of Experimental Example <1-1>, and then EDTA was added to prevent coagulation, followed by centrifugation at 3000 rpm for 10 minutes to obtain plasma. Samples were used and stored at −70 ° C. until analysis.

실험 결과는 SPSS/PC 9.0 프로그램을 이용하여 평균ㅁ 표준편차로 나타내었으며 그룹간의 유의적인 통계차를 분석하기 위하여 p<0.05의 유의수준에서 일원분산분석(one-way ANOVA test)을 실시한 다음 던칸 다중범위 검정(Duncan's multiple range test)를 이용하여 사후검증을 하였다.The experimental results were expressed as the mean standard deviation using the SPSS / PC 9.0 program, and one-way ANOVA test was performed at the significance level of p <0.05 to analyze the statistical difference between the groups. Post-test was performed using Duncan's multiple range test.

<2-1> 혈중 중성지방, 글루코오스 및 자유 지방산 농도<2-1> Blood Triglyceride, Glucose and Free Fatty Acid Concentration

지방은 체조직에 중성지방 형태로 축적되었다가 에너지 소비에 의해 자유 지방산 형태로 혈중에 유입된다(Jones NL et al., Am J Physiol, 213(4), 824-828, 1967). 따라서, 자유 지방산 농도는 에너지 소비 정도를 반영하게 된다. 또한, 혈중 글루코오스 농도는 에너지 소비 정도를 반영하는 인자로서 혈중 글루코오스 농도가 저하되면 피로감, 지구력 운동수행 능력이 저해된다. 이에 실험예 <1-1>의 각 군의 혈중 중성지방, 글루코오스 및 자유 지방산 농도를 각각 상업용 키트인 시그마사의 336-10, 510-DA 및 Sicdia Nefazyme(영연화학주식회사, Korea)를 사용하여 측정하였다.Fat accumulates in the form of triglycerides in body tissues and enters the blood in the form of free fatty acids by energy consumption (Jones NL et al., Am J Physiol , 213 (4), 824-828, 1967). Thus, the free fatty acid concentration will reflect the degree of energy consumption. In addition, blood glucose concentration is a factor reflecting the degree of energy consumption, and when blood glucose concentration is lowered, fatigue and endurance exercise performance are impaired. Therefore, the concentrations of triglyceride, glucose and free fatty acids in blood of each group of Experimental Example <1-1> were measured using 336-10, 510-DA and Sicdia Nefazyme (Yukyeon Chemical Co., Ltd.) of Sigma Co., Ltd., respectively. .

실험 결과, 비훈련 대조군과 훈련 대조군의 경우 혈중 글루코오스, 자유 지방산 및 중성지방 농도는 유의적인 차이를 나타내지 않았다. 또한, 훈련 대조군과 훈련 실험군의 경우 혈중 글루코오스 농도 및 중성지방 농도는 유의적인 차이를 나타내지 않았으나, 훈련 실험군의 자유 지방산 농도가 훈련 대조군에 비해 유의적으로 낮게 나타났다(p<0.05)(표 4). 이는 훈련 실험군이 혈중 자유 지방산을 빠르게 분해하여 에너지원으로 사용하였기 때문인 것으로 사료되었다.As a result, there was no significant difference in blood glucose, free fatty acid and triglyceride levels between the non-trained and the training control group. In addition, blood glucose concentration and triglyceride concentration did not show significant difference between the training control group and the training group, but the free fatty acid concentration of the training group was significantly lower than that of the training control group (p <0.05) (Table 4). This may be because the training group rapidly decomposed blood free fatty acids and used them as energy sources.

탈진시까지 강제수영시킨 실험동물의 혈중 글루코오스, 지방산 및 중성지방 농도(평균±표준편차)Blood Glucose, Fatty Acid, and Triglyceride Concentrations in Experimental Animals Forced to Exhaustion (mean ± standard deviation) 그룹group 글루코오스(mg/dL)Glucose (mg / dL) 유리 지방산(meq/L)Free fatty acids (meq / L) 중성지방(mg/dL)Triglycerides (mg / dL) 비훈련 대조군Untrained control group 41.71±16.5441.71 ± 16.54 800.86±86.64800.86 ± 86.64 137.10±31.02137.10 ± 31.02 훈련 대조군Training control 40.43±11.1540.43 ± 11.15 844.29±179.08844.29 ± 179.08 100.03±9.83100.03 ± 9.83 훈련 실험군Training experiment group 46.71±19.1146.71 ± 19.11 546.86±71.63* 546.86 ± 71.63 * 89.68±8.1189.68 ± 8.11

*: 훈련 대조군과 비교시 유의적인 차이가 있음(p<0.05).*: Significant difference compared to the training control (p <0.05).

<2-2> 혈중 젖산, 무기 인산, 크레아틴 키나제 및 암모니아 농도<2-2> Blood Lactic Acid, Inorganic Phosphate, Creatine Kinase and Ammonia Concentrations

운동시 체내에 축적되는 피로요소인 젖산, 근섬유의 교차교를 약화시켜 힘 생성능을 저하시키는 것으로 알려진 무기인산, 아미노산 분해로 인해 생성되는 피로요소인 암모니아 농도 및 체내에 원활한 에너지 공급이 어려운 경우에 그 활성이 증가되는 것으로 알려진 크레아틴 키나제의 활성을 실험예 <1-1>의 각 그룹을 대상으로 하여 측정하였다. 혈중 젖산, 무기인산, 암모니아 농도 및 크레아틴 키나제 활성을 각각 상업용 키트인 씨그마사의 735-10, BSC 무기인산 측정용 키트(Bio Clinical System Corporation, Korea), BSC 오토 CPK 키트(Bio Clinical System Corporation, Korea) 및 시그마사의 171-B를 사용하여 측정하였다.In case that it is difficult to supply energy to the body, it is difficult to supply the energy of inorganic phosphoric acid, which is known to weaken the cross bridge of lactic acid and muscle fiber, which are accumulated in the body during exercise, and the ammonia concentration, which is the fatigue factor produced by amino acid decomposition, and the body. The activity of creatine kinase known to increase in activity was measured for each group of Experimental Example <1-1>. Blood lactic acid, inorganic phosphate, ammonia concentration and creatine kinase activity were measured by Sigma Corporation's 735-10, BSC inorganic phosphate measurement kit (Bio Clinical System Corporation, Korea), and BSC Auto CPK kit (Bio Clinical System Corporation, Korea). ) And 171-B from Sigma.

실험 결과, 혈중 젖산 농도는 그룹 간에 유의적인 차이를 나타내지 않았다. 혈중 무기인산 농도는 훈련 대조군이 비훈련 대조군에 비해 낮은 경향을 보였으나 유의적인 차이는 없었다. 또한, 훈련 실험군의 경우에도 훈련 대조군과 비교시 유의적인 차이를 나타내지 않았다. 혈중 암모니아 농도는 비훈련 대조군에 비해 훈련 대조군에서 유의적으로 낮게 나타났다. 훈련 실험군의 경우에는 훈련 대조군과 비교시 유의적인 차이를 나타내지 않았다. 크레아틴 키나제 활성은 훈련 대조군의 경우 비훈련 대조군에 비해 유의적으로 낮은 활성을 나타냈다. 한편, 탈진시까지 강제 수영시킨 훈련 실험군의 경우 훈련 대조군에 비해 크레아틴 키나제 활성이 유의적인 차이를 나타내지 않았다(표 5). As a result, blood lactate concentration showed no significant difference between groups. Serum inorganic phosphate concentration tended to be lower in the control group than in the non-training control group, but there was no significant difference. In addition, the training group did not show a significant difference compared to the training control group. Blood ammonia levels were significantly lower in the training control group than in the non-training control group. There was no significant difference in the training group compared with the training control group. Creatine kinase activity was significantly lower in the training control group than in the non-training control group. On the other hand, the training group that was forced to swim until exhaustion did not show a significant difference in creatine kinase activity compared to the training control (Table 5).

한편, 훈련 실험군의 경우 다른 그룹에 비해 더 오랜 시간 운동을 수행하였음에도 불구하고 상기 훈련 실험군의 혈중 피로요소인 젖산, 무기인산 및 암모니아 농도가 다른 그룹과 유의적인 차이가 없는 것으로 나타났다. 따라서, 상기 결과로부터 본 발명에 따른 조성물의 섭취가 혈중 피로물질의 축적을 억제하는 효과가 있음을 알 수 있었다.On the other hand, the training experimental group was found to have no significant difference in the blood fatigue factor lactic acid, inorganic phosphate and ammonia concentration of the training group even though the exercise was performed longer than the other groups. Therefore, it can be seen from the above results that the ingestion of the composition according to the present invention has an effect of inhibiting accumulation of blood substances in the blood.

탈진시까지 강제 수영시킨 실험동물의 혈중 젖산, 무기인산, 암모니아 농도 및 크레아틴 키나제 활성Blood Lactate, Inorganic Phosphate, Ammonia Concentrations and Creatine Kinase Activity in Experimental Animals Forced to Swim 그룹group 젖산(mg/dL)Lactic Acid (mg / dL) 무기인산(mg/dL)Inorganic Phosphate (mg / dL) 암모니아(㎍/dL)Ammonia (µg / dL) 크레아틴 키나제(U/L)Creatine Kinase (U / L) 비훈련 대조군Untrained control group 87.83±38.2487.83 ± 38.24 13.67±1.5613.67 ± 1.56 360.26±59.71360.26 ± 59.71 326.00±84.13326.00 ± 84.13 훈련 대조군Training control 81.20±20.5081.20 ± 20.50 12.54±1.1412.54 ± 1.14 287.24±44.69+287.24 ± 44.69 + 179.54±31.47+ 179.54 ± 31.47 + 훈련 실험군Training experiment group 82.00±24.5582.00 ± 24.55 11.64±1.5611.64 ± 1.56 252.11±39.09252.11 ± 39.09 226.71±107.93226.71 ± 107.93

+: 비훈련 대조군과 비교시 유의적인 차이가 있음(p<0.05).+: Significant difference compared to non-trained control group (p <0.05).

<2-3> 간과 근육의 무게 및 글리코겐 함량 비교<2-3> Comparison of liver and muscle weight and glycogen content

상기 실험예 <1-1>의 실신시까지 강제 수영시킨 각 그룹의 랫트로부터 간, 비복근(gastrocnemius muscle) 및 가자미근(soleus muscle)을 적출한 후 액체 질소로 급속동결시켰다. 그 다음 글리코겐 농도를 분석할 때 까지 -70℃에서 보관하였다. 글리코겐 농도는 글루코오스 옥시다아제 방법에 따라 분광광도계로 측정하였다(Chun Y. et al. J Clin Microbiol 36:1981-1082, 1998). 즉, 일정량의 간과 근육을 30% KOH로 100℃에서 30분간 가수분해한 후 1.5mL의 무수 에탄올을 첨가하였다. 그 다음 4,000×g에서 15분간 원심분리하여 상층액을 버리고, 0.5mL의 증류수와 0.2% 안트론(anthrone) 1mL를 첨가하여 끓는물에서 20분간 반응시켰다. 이를 방냉한 후 표준 포도당 용액을 이용하여 620nm에서 비색정량을 실시하고 표준곡선으로부터 글리코겐 농도를 산출하였다.Liver, gastrocnemius muscle and soleus muscle were extracted from rats of each group forced to swim until the synch of the Experimental Example <1-1>, and then rapidly frozen with liquid nitrogen. It was then stored at −70 ° C. until glycogen concentration was analyzed. Glycogen concentrations were measured spectrophotometrically according to the glucose oxidase method (Chun Y. et al. J Clin Microbiol 36: 1981-1082, 1998). That is, a certain amount of liver and muscle was hydrolyzed with 30% KOH at 100 ° C. for 30 minutes and 1.5 mL of anhydrous ethanol was added thereto. Then, the supernatant was discarded by centrifugation at 4,000 × g for 15 minutes, and 0.5 mL of distilled water and 1 mL of 0.2% anthrone were added and reacted in boiling water for 20 minutes. After cooling, colorimetric measurement was performed at 620 nm using a standard glucose solution, and glycogen concentration was calculated from the standard curve.

실험 결과, 간 무게는 각 그룹 간에 유의적인 차이가 없었다(표 6). 이는 본 발명에 따른 식이 조성물의 농도 및 배합비가 실험동물의 체내에서 독성을 유발하지 않는 적합한 범위임을 간접적으로 나타내 주는 것이다. 근육 무게는 각 그룹 간에 유의적인 차이를 나타내지 않았다(표 6).As a result, liver weight was not significantly different between the groups (Table 6). This indirectly indicates that the concentration and the mixing ratio of the dietary composition according to the present invention are in a suitable range that does not cause toxicity in the body of the experimental animal. Muscle weight did not show a significant difference between each group (Table 6).

간 및 근육 무게Liver and muscle weight 그룹group 간 무게(g/체중 100g)Liver weight (g / weight 100g) 근육 무게(g/ 체중 100g)Muscle weight (g / weight 100g) 비복근Gastrocnemius 가자미근Soleus 비훈련 대조군Untrained control group 2.57±0.132.57 ± 0.13 0.60±0.020.60 ± 0.02 0.04±0.010.04 ± 0.01 훈련 대조군Training control 2.49±0.132.49 ± 0.13 0.59±0.030.59 ± 0.03 0.05±0.010.05 ± 0.01 훈련 실험군Training experiment group 2.56±0.112.56 ± 0.11 0.60±0.010.60 ± 0.01 0.04±0.010.04 ± 0.01

간과 근육의 글리코겐 농도는 훈련 실험군이 비훈련 대조군 및 훈련 대조군에 비해 높게 나타났으나 유의적인 차이는 없었다(표 7). 상기 글리코겐은 글루코오스의 체내 저장형태로서 강도 높은 운동을 반복하면 고갈한다. 따라서, 체내에 축적된 글리코겐을 최대한 절약하는 것은 운동능력을 향상시키는 필수적인 요소가 된다. 그러므로, 상대적으로 더 긴 시간 동안 운동을 한 훈련 실험군의 간과 근육 내 글리코겐 농도가 다른 그룹과 유의적인 차이를 나타내지 않았다는 것은 본 발명의 조성물을 섭취함으로써 체내에 축적된 글리코겐이 최대한 절약될 수 있다는 것을 의미한다.Glycogen concentrations in the liver and muscle were higher in the training group than in the non-training and training groups, but there was no significant difference (Table 7). The glycogen is depleted by repeated high-intensity exercise as a form of glucose storage in the body. Therefore, maximally saving glycogen accumulated in the body becomes an essential factor for improving athletic performance. Therefore, the fact that the liver and muscle glycogen concentrations of the training group who exercised for a relatively longer time did not show a significant difference from other groups, which means that the glycogen accumulated in the body can be saved as much as possible by taking the composition of the present invention. do.

간과 근육 내 글리코겐 농도Glycogen Concentrations in the Liver and Muscle 그룹group 간의 글리코겐 농도(mg/g tissue)Glycogen Concentration in Liver (mg / g tissue) 근육의 글리코겐 농도(mg/g tissue)Muscle glycogen concentration (mg / g tissue) 실신시까지 강제수영Forced swimming until fainting 실신시까지 강제수영Forced swimming until fainting 비훈련 대조군Untrained control group 16.73±0.8416.73 ± 0.84 0.30±0.050.30 ± 0.05 훈련 대조군Training control 16.91±0.9916.91 ± 0.99 0.31±0.040.31 ± 0.04 훈련 실험군Training experiment group 17.73±1.5517.73 ± 1.55 0.34±0.030.34 ± 0.03

<2-4> 근육 내 LDH 효소 활성<2-4> LDH Enzyme Activity in Muscle

젖산 디하이드로게나제(lactate dehydorgenase, LDH)는 무산소 상태에서 피루브산(pyruvate)으로부터 젖산의 형성을 촉매하는 효소이다(백일영 등, 한국체육학회지 36(1):281-233, 1998). 고강도 운동시 그 활성이 증가되며 근육 피로에 있어서 중요한 인자로 알려져 있다(Pesce A et al., J. Biol. Chem. 239:1753-1761, 1964; Medina R. et al., FEBS 180(1): 77-80, 1985).Lactate dehydrogenase The (dehydorgenase lactate, LDH) is an enzyme that catalyzes the formation of lactic acid from pyruvic acid (pyruvate) in oxygen-free conditions (such as baekilyoung, Physical Society of Korea 36 (1): 281-233, 1998). Its activity increases during high-intensity exercise and is known as an important factor in muscle fatigue (Pesce A et al., J. Biol. Chem . 239: 1753-1761, 1964; Medina R. et al., FEBS 180 (1) : 77-80, 1985).

근육의 LDH 활성은 공지의 방법을 수정하여 비복근에서 측정하였다(Peace A. et al., J. Biol. Chem. 239:1753-1761, 1964). 즉, 비복근을 상기 실험예 <2-3>과 동일한 방법으로 탈진시까지 강제 수영시킨 각 그룹의 랫트로부터 적출하고 100mM KHPO4 완충액에 가한 다음 폴리트론 균질화기(polytron homogenizer)를 이용하여 균질화하였다. 효소활성을 측정하기 위하여 100mM KHPO4 0.84mL, 3.3mM 나트륨· 피르브산 0.1mL에 상기 균질화한 액 0.02mL를 첨가한 후 3.6mM DPNH 0.04mL를 넣어 반응시키고 30℃를 유지하는 스펙트로포토미터를 사용하여 340nm에서의 흡광도 변화를 측정하였다. 효소 활성은 μmol/g tissue/min으로 나타내었다.LDH activity of muscle was measured in gastrocnemius muscle by modifying known methods (Peace A. et al., J. Biol. Chem . 239: 1753-1761, 1964). That is, the gastrocnemius muscle was extracted from rats of each group forced swimming until exhaustion in the same manner as in Experimental Example <2-3>, added to 100mM KHPO 4 buffer, and homogenized using a polytron homogenizer. To measure enzymatic activity, add 0.02 mL of the homogenized solution to 0.84 mL of 100 mM KHPO 4 and 0.1 mL of 3.3 mM sodium pirbic acid, add 0.04 mL of 3.6 mM DPNH, and use a spectrophotometer to maintain 30 ° C. The absorbance change at 340 nm was measured. Enzyme activity is expressed as μmol / g tissue / min.

실험 결과, 비훈련 대조군에 비해 훈련 대조군에서 유의적으로 높은 활성을 나타냈다. 훈련 실험군의 경우에는 훈련 대조군에 비해 약간 높은 활성을 나타냈으나 유의적인 차이를 나타내지 않았다(표 8).The experimental results showed significantly higher activity in the training control group than the non-training control group. In the training group, the activity was slightly higher than the training control group, but there was no significant difference (Table 8).

상기 훈련 실험군의 경우 다른 그룹에 비해 상대적으로 더 긴 시간 동안 운동을 수행하였음에도 불구하고 LDH 효소 활성이 더 낮거나 유의적으로 차이가 없는 것으로 나타났다. 따라서, 상기 결과로부터 본 발명에 따른 조성물이 근육의 피로를 지연시키는 효과가 있음을 알 수 있었다.The training group showed lower or no significant difference in LDH enzyme activity even though the training was performed for a relatively longer time than the other groups. Therefore, it can be seen from the above results that the composition according to the present invention has an effect of delaying muscle fatigue.

LDH 효소 활성LDH Enzyme Activity 그룹group 근육 내 LDH 효소 활성(μmol/g tissue/min)LDH enzyme activity in muscle (μmol / g tissue / min) 탈진시까지 강제 수영시킨 경우In case of forced swimming until exhaustion 비훈련 대조군Untrained control group 13.00±0.8413.00 ± 0.84 훈련 대조군Training control 13.23±1.25+ 13.23 ± 1.25 + 훈련 실험군Training experiment group 14.67±1.4814.67 ± 1.48

+: 비훈련 대조군과 비교시 유의적인 차이가 있음(p<0.05)+: Significant difference compared to non-trained control group (p <0.05)

<2-5> 근육 내 CS 효소 활성<2-5> Intramuscular CS Enzyme Activity

구연산 합성효소(citrate synthase, CS 효소)는 TCA 회로의 첫 단계에서 구연산의 합성을 촉매하는 효소이다. 일반적으로 유산소 운동에 의해 증가하는 것으로 알려져 있다(Mellandy J. et al., Method of enzymatic analysis, Bergmeyer HU ed., 4:1840-1843, 1974).Citric acid synthase (CS enzyme) is an enzyme that catalyzes the synthesis of citric acid in the first stage of the TCA cycle. It is generally known to increase by aerobic exercise (Mellandy J. et al., Method of enzymatic analysis, Bergmeyer HU ed., 4: 1840-1843, 1974).

상기 CS 효소 활성은 공지의 방법을 수정하여 가자미근에서 측정하였다(Srere PA, Method Enzymol, 13:3-11, 1969). 즉, 가자미근을 상기 실험예 <2-3>과 동일한 방법으로 적출하고 0.175M KCl 완충액에 첨가한 후 폴리트론 균질화기를 이용하여 균질화하였다. 상기 균질화액 중의 미토콘드리아막을 파괴하기 위하여 동결과 융해를 3회 반복하여 시료로 사용하였다. 효소 활성을 측정하기 위하여 100mM Tris-base 완충액 0.33mL, 1mM DTNB 0.05mL, 3mL 아세틸 CoA 0.08mL 및 상기 시료액 0.01mL를 혼합한 후 10mM 옥살로아세테이트 0.03mL를 첨가하여 반응시키고 30℃를 유지하는 스펙트로포토미터를 이용하여 412nm에서의 흡광도를 측정하였다. 효소 활성은 μmol/g tissue/min으로 나타내었다.The CS enzyme activity was measured in the soleus muscle by modifying a known method (Srere PA, Method Enzymol, 13: 3-11, 1969). That is, the flounder muscle was extracted in the same manner as in Experimental Example <2-3> and added to 0.175M KCl buffer, and homogenized using a polytron homogenizer. In order to break the mitochondrial membrane in the homogenate, freezing and thawing were used three times as samples. In order to measure enzyme activity, 0.33 mL of 100 mM Tris-base buffer, 0.05 mL of 1 mM DTNB, 0.08 mL of 3 mL acetyl CoA, and 0.01 mL of the sample solution were mixed, followed by addition of 0.03 mL of 10 mM oxaloacetate and maintained at 30 ° C. Absorbance at 412 nm was measured using a spectrophotometer. Enzyme activity is expressed as μmol / g tissue / min.

실험 결과, 비훈련 대조군의 효소 활성은 훈련 대조군에 비해 유의적으로 높게 나타났으며 훈련 실험군의 경우에는 훈련 대조군과 유의적인 차이를 나타내지 않았다(표 9). 상기 훈련 실험군의 경우 다른 그룹에 비해 상대적으로 더 긴 시간 동안 운동을 수행하였음에도 불구하고 CS 효소 활성이 유의적으로 차이가 없는 것으로 나타났다. 상기 결과로부터 본 발명에 따른 조성물이 근육의 피로를 지연시키는 효과가 있음을 알 수 있었다.As a result, the enzyme activity of the non-trained control group was significantly higher than that of the training control group, and the training group did not show a significant difference from the training control group (Table 9). In the training group, the CS enzyme activity was not significantly different even though the training was performed for a relatively longer time than the other groups. From the above results, it can be seen that the composition according to the present invention has an effect of delaying muscle fatigue.

CS 효소 활성CS enzyme activity 그룹group 근육 내 CS 효소 활성(μmol/g tissue/min)Intramuscular CS enzyme activity (μmol / g tissue / min) 탈진시까지 강제 수영시킨 경우In case of forced swimming until exhaustion 비훈련 대조군Untrained control group 28.02±2.6228.02 ± 2.62 훈련 대조군Training control 22.77±2.52+ 22.77 ± 2.52 + 훈련 실험군Training experiment group 21.32±1.4421.32 ± 1.44

+: 비훈련 대조군과 비교시 유의적인 차이가 있음(p<0.05)+: Significant difference compared to non-trained control group (p <0.05)

<실시예 2><Example 2>

본 발명에 따른 운동수행능력 증강용 음료 조성물의 제조Preparation of a beverage composition for enhancing athletic performance according to the present invention

상기 실시예 1에서 제조한 조성물 28.8g, 구연산 0.16g, 고과당 12g, 향료 0.1g을 혼합하고 여기에 음용수를 첨가하여 최종 부피가 120mL가 되도록 하였다. 이를 파우치에 넣어 밀봉한 다음 100℃에서 30분간 가열멸균하고 냉각함으로써 음료 조성물을 제조하였다.28.8 g of the composition prepared in Example 1, 0.16 g of citric acid, 12 g of high fructose, and 0.1 g of fragrance were mixed, and drinking water was added thereto so that the final volume was 120 mL. It was put into a pouch and sealed, and then heat sterilized at 100 ° C. for 30 minutes to prepare a beverage composition.

본 발명에 따른 조성물은 장기간 섭취시 운동수행시간을 증진시키며 운동시 혈중 피로물질의 축적을 억제하는 효과가 있어서 운동선수의 체력 및 운동능력 증진에 유용하다.The composition according to the present invention is useful for enhancing physical fitness and athletic ability of the athlete because it has an effect of improving exercise performance time when prolonged ingestion and inhibiting accumulation of blood fatigue substances during exercise.

Claims (4)

스쿠알렌 25∼35중량%, 삼백초 수추출물 20∼25중량%, 오가피 수추출물 20∼25중량% 및 동충하초 수추출물 20∼25중량%로 이루어진 조성물 100 중량부에 대하여 콜라넛 분말 또는 추출물 10∼20중량부, 타우린 10∼20중량부, 포스파티딜콜린 1∼5중량부, 글루타민 5∼10중량부, L-아르기닌 5∼10중량부 및 L-카르니틴 5∼10중량부를 추가로 포함하는 것을 특징으로 하는 운동수행능력 증강용 조성물.Colanut powder or extract 10 to 20% by weight based on 100 parts by weight of a composition consisting of 25 to 35% by weight squalene, 20 to 25% by weight water extract, 20 to 25% by weight Ogapi water extract and 20 to 25% by weight Cordyceps sinensis extract Performing an exercise, characterized in that it further comprises 10 to 20 parts by weight of taurine, 1 to 5 parts by weight of phosphatidylcholine, 5 to 10 parts by weight of glutamine, 5 to 10 parts by weight of L-arginine and 5 to 10 parts by weight of L-carnitine. Composition for enhancing ability. 삭제delete 제1항의 조성물을 포함하는 음료.A beverage comprising the composition of claim 1. 제3항에 있어서, 제1항의 조성물 20∼25중량%, 구연산 0.1∼0.2중량%, 고과당 5∼15중량%, 향료 0.05∼0.1중량% 및 잔여량의 음용수로 이루어진 음료.The beverage according to claim 3, comprising 20 to 25% by weight of the composition of claim 1, 0.1 to 0.2% by weight citric acid, 5 to 15% by weight high fructose, 0.05 to 0.1% by weight fragrance, and a residual amount of drinking water.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014058160A1 (en) 2012-10-11 2014-04-17 포항공과대학교 산학협력단 Composition comprising myricetin as active ingredient for enhancing exercise performance or fatigue recovery
KR20140089501A (en) 2008-02-19 2014-07-15 주식회사 유니젠 Composition for improvement of exercise performance, fatigue recovery and antioxidation activity comprising processed Panax species plant leaf extract
CN104223290A (en) * 2014-07-30 2014-12-24 安徽省百益食品有限公司 Pleurotus geesteranus and oyster beverage and preparation method thereof
WO2018026211A1 (en) * 2016-08-03 2018-02-08 (주)앗코스텍 Composition containing squalene for improving muscle function and preventing muscle damage
KR101830395B1 (en) * 2016-08-03 2018-02-20 (주)앗코스텍 Composition comprising squalene for enhancement of muscle function and prevention of muscle damage

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140089501A (en) 2008-02-19 2014-07-15 주식회사 유니젠 Composition for improvement of exercise performance, fatigue recovery and antioxidation activity comprising processed Panax species plant leaf extract
WO2014058160A1 (en) 2012-10-11 2014-04-17 포항공과대학교 산학협력단 Composition comprising myricetin as active ingredient for enhancing exercise performance or fatigue recovery
CN104223290A (en) * 2014-07-30 2014-12-24 安徽省百益食品有限公司 Pleurotus geesteranus and oyster beverage and preparation method thereof
WO2018026211A1 (en) * 2016-08-03 2018-02-08 (주)앗코스텍 Composition containing squalene for improving muscle function and preventing muscle damage
KR101830395B1 (en) * 2016-08-03 2018-02-20 (주)앗코스텍 Composition comprising squalene for enhancement of muscle function and prevention of muscle damage

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