KR100526163B1 - Composition for enhancing exercise performance - Google Patents

Abstract

The present invention relates to a composition for enhancing athletic performance. More specifically, the present invention relates to a composition for enhancing exercise performance, including medium chain triglyceride, octacosanol and plant extracts. The composition according to the present invention is useful for improving physical fitness and athletic ability of the athlete because it has an effect of enhancing exercise performance time and prohibiting accumulation of blood fatigue substances during long-term ingestion.

Description

Composition for enhancing exercise performance

The present invention relates to a composition for enhancing athletic performance. More specifically, the present invention relates to a composition for enhancing exercise performance, including medium chain triglyceride, octacosanol, and plant extracts, which enhances exercise performance and prolongs accumulation of blood fatigue substances in long-term intake. It has a inhibitory effect and relates to a composition useful for enhancing the fitness and athletic performance of an athlete.

Athletes always strive to their fullest strength and skills to set new records. For this purpose, scientific training, diet, technology and equipment improvement, and ergogenic aids are used. Among them, exercise aids are used not only for athletes, but also for the elimination of fatigue factors that accumulate in the body and cause fatigue.

Research on functional supplements for improving athletic performance is being actively conducted in both East and West. On the other hand, supplements containing compounds such as steroids, caffeine, sodium bicarbonate and sodium citrate can significantly increase exercise performance when taken in a certain amount, but such drugs may have fatal side effects and ultimately damage health. There is this. Therefore, in recent years, researches are being actively conducted to develop functional supplements by using natural products having a guaranteed safety such as plant extracts.

The present inventors while studying the supplements without side effects while effectively enhancing the exercise performance, preparing a mixed composition consisting of food additives and plant extracts and analyzed the exercise performance according to the intake of the mixed composition When the mixed composition is ingested for a long time, the present invention was completed by confirming that exercise performance was increased and blood accumulation of accumulation of fatigue elements was suppressed.

Accordingly, it is an object of the present invention to provide a composition for enhancing exercise performance including heavy chain triglycerides, octacosanol and plant extracts.

In order to achieve the above object, the present invention provides a composition for enhancing exercise performance, including heavy chain triglycerides, octacosanol and plant extracts.

Hereinafter, the present invention will be described in detail.

The composition according to the present invention is 75 to 85% by weight of medium chain triglycerides, 0.5 to 1.0% by weight of octacosanol, 5 to 15% by weight of red ginseng extract, 1 to 5% by weight of Bokbunja water extract, and 1 ~ Ogapi water extract. It is characterized by consisting of 5% by weight. Preferably, the composition according to the present invention may be composed of 79.4% by weight of medium-chain triglycerides, 0.7% by weight of octacosanol, 11.9% by weight of red ginseng extract, 4.0% by weight of Bokbunja water extract, and 4.0% by weight of Ogapi water extract. The water extract of red ginseng, bokbunja and agapi can be prepared and used according to a known method. Alternatively, the water extracts may be purchased and used commercially. For example, drinking water is added to the red ginseng, bokbunja and ogapi in the amount of 2 to 10 times by weight and heated at 80 to 100 ° C for 20 to 60 minutes, or purchased from Dasong Food Raw Materials Co., Ltd. Can be used.

The medium-chain triglycerides have 6 to 12 carbon skeletons, which are more easily oxidized in cells because of their smaller size than the long-chain fats, and are known to produce more calories after ingestion. . In addition, the heavy chain triglycerides have the advantage of generating energy more easily than carbohydrates while reducing the secretion of insulin hormones.

Octacosanol is a kind of chemically stable white crystalline saturated aliphatic alcohol having 28 carbon atoms and having 1 hydroxyl group. The octacosanol is contained in traces such as tangerine, wheat and rice embryos, alpha waves, apples, grape peels, sugar cane and the like. In general, octacosanol is prepared by extracting, concentrating and purifying from embryonic oil of wheat and rice. The physiological activity of the octacosanol is known to improve endurance, stamina, stamina, improve the reaction time, improve the glycogen storage rate of muscle and lower systolic blood pressure.

Red ginseng is a red ginseng dried by steaming ginseng, and it has a sedative effect and excitement effect on the central nervous system, and acts on the circulatory system to prevent hypertension or arteriosclerosis. In addition, red ginseng lowers hematopoietic and blood sugar levels, protects the liver, and acts indirectly on sexual and reproductive effects by acting on the endocrine system, and has anti-inflammatory and anti-tumor effects. It also protects and softens the skin.

Bokbunja has been used in the private sector for the treatment of jeongjeong, soft water, gegal, tonic and diabetes. Bokbunja's nutritional sources are high in minerals such as phosphorus, iron and potassium, especially organic acids and vitamin C, and phenolic compounds.

Ogapi refers to dried roots and bark of the bark of deciduous tree, arthropod and deciduous tree, and is known to be prescribed for stomach, arthritis, back pain, degenerative arthritis syndrome, species, each, bruise and swelling.

In addition, the composition for enhancing exercise performance according to the present invention may further include a cola nut (Cola nut) powder or extract, taurine, phosphatidylcholine, glutamine, L-arginine and L-carnitine. Preferably, the composition for enhancing exercise performance according to the present invention 1 to 5 parts by weight of the collagen powder or extract, taurine 1 to 5 with respect to 100 parts by weight of the mixed composition consisting of the medium-chain triglycerides, octacosanol and plant extracts Weight parts, 0.1 to 1.0 parts by weight of phosphatidylcholine, 1.5 to 2.5 parts by weight of glutamine, 1.5 to 2.5 parts by weight of L-arginine and 1.5 to 2.5 parts by weight of L-carnitine may be further included. More preferably, it may further comprise 4.0 parts by weight of the collagen powder or extract, 4.0 parts by weight of taurine, 0.5 parts by weight of phosphatidylcholine, 1.9 parts by weight of glutamine, 1.9 parts by weight of L-arginine and 1.9 parts by weight of L-carnitine.

Amino acids such as taurine, glutamine, L-arginine and L-carnitine may be used as direct energy sources to help muscle fatigue after exercise.

The phosphatidylcholine is present in egg yolk, soybean oil, liver and brain as a compound of fat, phosphorus and nitrogen. It is one of the important components of cell membrane composition and is known for fatigue recovery.

The colanut belongs to the cactus (Sterculiaceae) and refers to the fruit containing the caffeine of Cola acuminata ( Cla nitida ) and C. nitida ( C. nitida ). Native to tropical Africa, it is used as a raw material for non-alcoholic beverages and medicines and as a herbal medicine to treat drug addiction, hangover and diarrhea.

The collagen contained in the composition of the present invention may be in the form of an extract or powder form. The collagen extract and the collagen powder are commercially available from Samjungyang Co., Ltd. can be used.

In one embodiment of the present invention, medium chain triglyceride 20g, octacosanol 0.18g, red ginseng water extract 3.0g, bokbunja water extract 1.0g, ogapi water extract 1.0g, colanut extract 1.0g, taurine 1.0g, phosphatidylcholine 0.1g, glutamine 0.5 g, 0.5 g L-arginine and 0.5 g L-carnitine were mixed to prepare a composition according to the present invention.

Furthermore, the present inventors mixed the composition of the present invention with the experimental dietary composition in order to measure the effect of exercise performance according to the intake of the composition according to the present invention to perform a forced swimming after a long intake in the rat maximum swimming capacity Was analyzed (see Experimental Example 1). As a result, the rats ingested the composition of the present invention was found to significantly increase the exercise performance time compared to the control group (see Table 3).

In addition, the present inventors investigated changes in blood and muscle fatigue factors after exercise according to the ingestion of the composition of the present invention (see Experimental Example 2). Blood fatigue factors were measured by blood glucose, free fatty acids, triglycerides, lactic acid, inorganic phosphate, ammonia concentration and creatine kinase activity. As muscle fatigue factors, glycogen concentration, lactate dehydrogenase activity, and citric acid synthase activity were measured. As a result of the experiment, the blood and intramuscular fatigue factor of the composition of the present invention showed no significant difference or lower than that of the control group not receiving the composition of the present invention.

From this, the inventors have found that by ingesting the composition of the present invention, the accumulation of fatigue factors due to exercise performance is suppressed, thereby extending the exercise performance time.

Preferred intake of the composition according to the present invention has been shown to have the effect of improving the exercise performance in the above is not particularly limited, 1.8 to 2.4g / weight kg, preferably about 1.85g / once per person Weight kg.

In addition, the composition according to the present invention can be prepared in the form of tea, juice and drink to drink, or ingested by granulation, encapsulation and powdered. In addition, the composition of the present invention can be prepared in the form of a mixed composition with a known component known as an adjuvant to enhance athletic performance.

Preferably, the composition of the present invention may be provided in the form of a drink which is easy to take. The exercise performance enhancing beverage according to the present invention may contain 20-25% (w / v), more preferably 24% (w / v) of the composition for improving athletic performance of the present invention. The beverage composition may be prepared by adding a conventionally used ingredients such as sweeteners, flavorings, citric acid, sodium bicarbonate, saline and drinking water in an appropriate amount. Oligosaccharides, fructose, high fructose, glucose, dextrin and fructooligosaccharides may be used as the sweetener. In addition, a small amount of carbon dioxide may be injected and prepared according to the preference of the drinker. In the case of citric acid and high fructose, the pH of the beverage may be added to about 3.2. More preferably, the beverage composition of the present invention is a composition containing 20 to 25% (w / v) of citric acid, octacosanol and plant extracts, 0.1 to 0.2% of citric acid (w / v), high fructose 5 It may contain ˜15% (w / v), flavor 0.05–0.1% (w / v) and residual drinking water. Preferably, the composition for enhancing athletic performance according to the present invention 24% (w / v), citric acid 0.13% (w / v), high fructose 10% (w / v), fragrance 0.08% (w / v) and The remaining amount of drinking water may be included.

In one embodiment of the present invention, 28.8 g of the composition for enhancing athletic performance of the present invention is mixed with 0.16 g of citric acid, 12 g of high fructose, and 0.1 g of fragrance, and the drinking water is added to make the total volume 120 ml and then put in a pouch. Sealed, heat sterilized at 100 ° C. for 30 minutes, and cooled to prepare a beverage composition (see Example 2).

Furthermore, the present inventors performed a human body experiment on physical education majors in order to confirm the exercise performance enhancing effect of the beverage composition prepared in one embodiment of the present invention (see Experimental Example 3 and Experimental Example 4).

As a result, according to the ingestion of the beverage composition of the present invention, the endurance exercise time was significantly increased compared to before ingestion, and the concentration of blood urea was found to be not significantly different than before ingestion.

From the above test results, the inventors found that the composition for enhancing exercise performance according to the present invention has an effect of prolonging exercise performance time by inhibiting accumulation of fatigue substances in blood.

Therefore, the composition for enhancing athletic performance according to the present invention may be used as an athletic aid to be useful for improving physical strength and athletic performance of an athlete or as an auxiliary for fatigue recovery in the general public.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

<Example 1>

Preparation of composition for enhancing athletic performance according to the present invention

Composition for enhancing exercise performance according to the present invention is 20g (79.4% by weight) medium chain fat, 0.18g (0.7% by weight) octacosanol, red ginseng extract 3.0g (11.9% by weight), 1.0g (4.0% by weight) bokbunja water extract ), 1.0 g (4.0 wt%) of Ogapi water extract, followed by 4.0 parts (1.0 g) of collagen extract, 4.0 parts (1.0 g) of taurine and 0.5 parts (0.1 g) of phosphatidylcholine, based on 100 parts by weight of the mixture. ), 1.9 parts by weight of glutamine (0.5 g), 1.9 parts by weight of L-arginine (0.5 g) and 1.9 parts by weight (0.5 g) of L-carnitine were further mixed. Red ginseng, Bokbunja and Ogapi extracts were purchased from Dasong Food Ingredients Co., Ltd. and colanut extracts were purchased from Samjeong Spice Company.

Experimental Example 1

Confirmation of the effect of enhancing exercise performance according to the intake of the composition of the present invention

The effect of enhancing exercise performance in rats according to the ingestion of the composition prepared in Example 1 was investigated. The experimental results were expressed as mean ± standard deviation using the SPSS / PC 9.0 program. One-way ANOVA test was performed at the significance level of p <0.05 to analyze the statistical difference between the groups. Post-test was performed using Duncan's multiple range test.

<1-1> Breeding and swimming training of experimental animals

Five-week-old male SD rats (Sprague-Dawley) were preliminarily bred for one week and 20 animals were randomly divided into three groups. That is, it is divided into a non-trained control group (Sedentary control group), a training control group (exercised control group) and a training experiment group (exercised formula B group), and the non-training control group and a training control group AIN-93 (Central Experimental Animal, Ansan, Korea) was freely ingested and the training group was ingested for 4 weeks the diet added to the composition prepared in Example 1 to the AIN-93 (Table 1). The total weight of the diet fed to the training group was adjusted to the same as other groups by adjusting the content of soybean oil and starch. Drinking water was freely consumed for all groups. In addition, except for the non-trained control group among the three groups, the swimming control training group and the training experiment group were conducted twice a week for adaptive training for 4 weeks. Training method for swimming is 10 minutes in week 1, 15 minutes in week 2, 20 minutes in week 3 and 25 in week 4 using a water tank (size 90 × 45 × 45 cm, water height 50 cm, water temperature 33-35 ° C). Incremental exercise load was used to perform a minute swim training. After four weeks of swimming training and dietary benefits were completed, forced swimming was performed until exhaustion. Animal environment was maintained at a temperature of 23 ± 1 ° C., a humidity of 50 ± 5%, and a 12-hour light and dark cycle.

ingredient Addition amount per group (g / kg) Untrained control group Training control Training experiment group Corn starch 367.486 367.486 383.852 Dextrinized corn starch 132 132 132 Sucrose 100 100 100 Casein 200 200 200 L-cystine 3 3 3 Soybean oil 100 100 38.034 Mineral mixtures 35 35 35 Vitamin mixtures 10 10 10 Cellulose powder 50 50 50 Choline bitartate 2.5 2.5 2.5 tert-butylhydroquinone 0.014 0.014 0.014 Composition of Example 1 - - 45.6 Total amount 1000 1000 1000

1) Addition amount is the addition amount of raw material per kg of feed (g).

<1-2> Body weight change and dietary efficiency of experimental animals

The weight change and dietary efficiency of the experimental animals of Experimental Example <1-1> were measured for 4 weeks. Body weight was measured once a week, dietary efficiency was measured using the following equation.

Dietary efficiency = weight gained during the experiment / amount of diet taken during the experiment

As a result of the experiment, the body weight at the beginning and the end of the experiment did not show a significant difference between the non-training control group and the training control group, but the training group showed a significantly lower body weight at the end of the experiment than the training control group (Table 2). Dietary efficiency did not show a significant difference between the groups.

Weight Changes and Dietary Efficiency of Experimental Animals group Weight (g) Body weight gained (g / 4 weeks) Dietary efficiency At the start of the experiment At the end of the experiment Untrained control group 198.51 ± 8.71 377.61 ± 24.98 179.11 ± 23.51 0.28 ± 0.03 Training control 196.30 ± 6.37 376.21 ± 25.96 179.93 ± 25.08 0.26 ± 0.08 Training experiment group 198.39 ± 6.93 348.71 ± 40.64 ^* 150.34 ± 43.19 ^* 0.24 ± 0.06

*: Significant difference compared to the training control (p <0.05).

<1-3> Measurement of maximum swimming capacity

Maximum swimming capacity was measured by recording the time until the rats forced to swim until each group exhausted in the Experimental Example <1-1> to sink to the bottom and do not rise back to the surface within 10 seconds.

As a result, there was no significant difference between the non-trained and the training control group. On the other hand, the swimming time was significantly increased in the training group compared to the training control group (Table 3). Therefore, it was found that the intake of the composition according to the present invention can enhance exercise performance time.

Swimming time of experimental animals group Swimming time (minutes) Untrained control group 154.29 ± 19.21 Training control 192.71 ± 48.73 Training experiment group 267.57 ± 48.46 ^*

*: Significant difference compared to the training control (p <0.05).

Experimental Example 2

Analysis of Fatigue Factors in Blood and Muscle According to Ingestion of Compositions of the Present Invention

Blood was collected from the heart of rats of each group of Experimental Example <1-1> and EDTA was added to prevent coagulation, and then plasma was obtained by centrifugation at 3000 rpm for 10 minutes, and then used as an analytical sample. Store at -70 ° C until.

All experimental results were expressed as mean ± standard deviation using the SPSS / PC 9.0 program. One-way ANOVA test was performed at the significance level of p <0.05 to analyze the statistical difference between groups. Post-validation was performed using the Duncan's multiple range test.

<2-1> Determination of Triglyceride, Glucose and Free Fatty Acid Concentrations in Blood

Fat accumulates in the form of triglycerides in body tissues and enters the blood in the form of free fatty acids by energy consumption (Jones NL et al., Am J Physiol , 213 (4), 824-828, 1967). Thus, the free fatty acid concentration will reflect the degree of energy consumption. In addition, blood glucose concentration is a factor reflecting the degree of energy consumption, and when blood glucose concentration is lowered, fatigue and endurance exercise performance are impaired. Therefore, the concentrations of triglycerides, glucose and free fatty acids in blood of each group forced to swim until exhaustion of Experimental Example <1-1> were respectively measured by 336-10, 510-DA and Sicdia Nefazyme of Sigma, a commercial kit. ) Was measured.

       As a result, the blood glucose and free fatty acid concentrations of the non-trained control group and the training control group were not significantly different, but the triglyceride concentration was significantly lower than the non-trained control group. There was no significant difference in blood glucose and triglyceride concentrations between the training control group and the training group, but the free fatty acid concentration of the training group was significantly lower than that of the training control group (Table 4).

This tendency is attributed to the fact that in the training experimental group, as the exercise time is extended compared to the other groups, rapid lipolysis action occurs, which promotes the conversion of triglycerides to free fatty acids, which are consumed as energy sources.

Blood Glucose, Fatty Acid and Triglyceride Concentrations in Experimental Animals Forced to Swim Until Depletion (Mean ± Standard Deviation) group Glucose (mg / dL) Free fatty acids (meq / L) Triglycerides (mg / dL) Untrained control group 41.71 ± 16.54 800.86 ± 86.64 137.10 ± 31.02 Training control 40.43 ± 11.15 844.29 ± 179.08 100.03 ± 9.83 ⁺ Training experiment group 36.57 ± 10.10 599.14 ± 62.81 ^* 93.79 ± 17.62

+: Significant difference compared to non-trained control group (p <0.05)

*: Significant difference compared to the training control group (p <0.05)

<2-2> Determination of Lactic Acid, Inorganic Phosphate, Ammonia and Creatine Kinase Activity in Blood

In case that it is difficult to supply energy to the body, it is difficult to supply the energy of inorganic phosphoric acid, which is known to weaken the cross bridge of lactic acid and muscle fiber, which are accumulated in the body during exercise, and the ammonia concentration, which is the fatigue factor produced by amino acid decomposition, The activity of creatine kinase known to increase in activity was measured for each group forced to swim until exhaustion of Experimental Example <1-1>. Blood lactic acid, inorganic phosphate, ammonia concentration and creatine kinase activity were measured by commercial kit Sigma's 735-10, BSC inorganic phosphate measurement kit (Bio Clinical System Corporation, Korea), BSC auto CPK kit (Bio Clinical System Corporation, Korea) ) And 171-B from Sigma.

As a result, blood lactate concentration did not show a significant difference between the groups (Table 5).

Serum inorganic phosphate concentration tended to be lower in the control group than in the non-training control group, but there was no significant difference. In addition, the training group did not show a significant difference compared to the training control group (Table 5).

The blood ammonia concentration was significantly higher in the non-trained control group than in the training control group, and the training group did not show any significant difference from the training control group (Table 5).

On the other hand, in the training group, although the exercise was performed for a longer time than the other groups, the blood fatigue factor, lactic acid, inorganic phosphate, and ammonia concentrations of the training group were not significantly different or lower than those of the other groups. Therefore, it can be seen from the experimental results that the intake of the composition according to the present invention has an effect of inhibiting the accumulation of blood substances in the blood.

Creatine kinase activity was significantly lower in the training control group than in the non-training control group. On the other hand, the training experimental group showed significantly higher creatine kinase activity than the training control group (Table 5). This is considered to be due to the body compensation mechanism for smooth supply of ATP in the training experimental group that performed the exercise for a relatively longer time than the training control group.

Blood Lactate, Inorganic Phosphate, Ammonia Concentrations and Creatine Kinase Activity in Experimental Animals Forced to Swim group Lactic Acid (mg / dL) Inorganic Phosphate (mg / dL) Ammonia (µg / dL) Creatine Kinase (U / L) Untrained control group 87.83 ± 38.24 13.67 ± 1.56 360.26 ± 59.71 326.00 ± 84.13 Training control 81.20 ± 20.50 12.54 ± 1.14 287.24 ± 44.69 ⁺ 179.54 ± 31.47 ⁺ Training experiment group 94.76 ± 27.77 12.86 ± 1.42 302.19 ± 47.90 236.43 ± 22.45 ^*

+: Significant difference compared to non-trained control group (p <0.05).

*: Significant difference compared to the training control (p <0.05).

<2-3> Comparison of liver and muscle weight and glycogen content

Liver, gastrocnemius muscle, and soleus muscle were extracted from rats of each group forced to swim until exhaustion of Experimental Example <1-1>, followed by rapid freezing with liquid nitrogen. It was then stored at −70 ° C. until glycogen concentration was analyzed. Glycogen concentrations were measured spectrophotometrically according to the glucose oxidase method (Chun Y. et al. J Clin Microbiol 36: 1981-1082, 1998). That is, a certain amount of liver and muscle was hydrolyzed with 30% KOH at 100 ° C. for 30 minutes and 1.5 mL of anhydrous ethanol was added thereto. Then, the supernatant was discarded by centrifugation at 4,000 × g for 15 minutes, and 0.5 mL of distilled water and 1 mL of 0.2% anthrone were added and reacted in boiling water for 20 minutes. After cooling, colorimetric measurement was performed at 620 nm using a standard glucose solution, and glycogen concentration was calculated from the standard curve.

As a result, liver weight was not significantly different between the groups (Table 6). This indirectly indicates that the concentration and the mixing ratio of the dietary composition according to the present invention are in a suitable range that does not cause toxicity in the body of the experimental animal. The muscle weight of gastrocnemius muscle was not significantly different between the non-training control group and the training control group, but the training group was significantly higher than the training control group. In the case of soleus muscle, the training control group was significantly higher than the non-training control group, and the training experimental group was not significantly different from the training control group (Table 6). The higher the weight of the muscle in the above means that the greater the amount of muscle can exercise longer. Therefore, the composition according to the present invention was found to be able to enhance the muscle performance by enhancing muscle mass.

Liver and muscle weights of experimental animals forced to swim until exhaustion group Liver weight (g / weight 100g) Muscle weight (g / weight 100g) Gastrocnemius Soleus Untrained control group 2.73 ± 0.50 0.61 ± 0.05 0.04 ± 0.01 Training control 2.62 ± 0.18 0.60 ± 0.04 0.05 ± 0.01 ⁺ Training experiment group 2.54 ± 0.17 0.63 ± 0.04 ^* 0.05 ± 0.01

+: Significantly different than the non-trained control group (p <0.05)

*: Significant difference compared to the training control group (p <0.05)

Glycogen concentrations in the liver and muscle were higher in the training group than in the non-training and training groups, but there was no significant difference (Table 7). The glycogen is depleted by repeated high-intensity exercise as a form of glucose storage in the body. Therefore, maximally saving glycogen accumulated in the body becomes an essential factor for improving athletic performance. Thus, the fact that the liver and muscle glycogen concentrations of the training group who exercised for a relatively longer time did not show a significant difference from the other groups, which means that the glycogen accumulated in the body can be saved as much as possible by taking the composition of the present invention. do.

Glycogen Concentrations in Liver and Muscle of Experimental Animals Forced to Exhaustion group Glycogen Concentration in Liver (mg / g tissue) Muscle glycogen concentration (mg / g tissue) Untrained control group 16.73 ± 0.84 0.30 ± 0.05 Training control 16.91 ± 0.99 0.31 ± 0.04 Training experiment group 19.37 ± 3.08 0.29 ± 0.06

<2-4> LDH Enzyme Activity in Muscle

Lactate dehydrogenase The (dehydorgenase lactate, LDH) is an enzyme that catalyzes the formation of lactic acid from pyruvic acid (pyruvate) in oxygen-free conditions (such as baekilyoung, Physical Society of Korea 36 (1): 281-233, 1998). Its activity increases during high-intensity exercise and is known as an important factor in muscle fatigue (Pesce A et al., J. Biol. Chem . 239: 1753-1761, 1964; Medina R. et al., FEBS 180 (1) : 77-80, 1985).

LDH activity of muscle was measured in gastrocnemius muscle by modifying known methods (Peace A. et al., J. Biol. Chem . 239: 1753-1761, 1964). That is, the gastrocnemius muscle was extracted from rats of each group forced to swim until exhaustion in the same manner as in Experimental Example <2-3>, added to 100 mM KHPO4 buffer, and homogenized using a polytron homogenizer. To measure enzymatic activity, add 0.02 mL of the homogenized solution to 0.84 mL of 100 mM KHPO ₄ and 0.1 mL of 3.3 mM sodium pyrvic acid, add 0.04 mL of 3.6 mM DPNH, and use a spectrophotometer to maintain 30 ° C. The absorbance change at 340 nm was measured. Enzyme activity is expressed as μmol / g tissue / min.

The experimental results showed significantly higher activity in the training control group than the non-training control group. In the training group, the activity was slightly higher than the training control group, but there was no significant difference (Table 8).

In the training group, the LDH enzyme activity was not significantly different even though the training was performed for a relatively longer time than the other groups. Therefore, it can be seen from the above results that the composition according to the present invention has an effect of delaying muscle fatigue.

LDH Enzyme Activity in Experimental Animals Forced to Swimming group LDH enzyme activity in muscle (μmol / g tissue / min) Untrained control group 13.00 ± 0.84 Training control 13.23 ± 1.25 ⁺ Training experiment group 13.63 ± 1.21

+: Significant difference compared to non-trained control group (p <0.05)

<2-5> Intramuscular CS Enzyme Activity

Citric acid synthase (CS enzyme) is an enzyme that catalyzes the synthesis of citric acid in the first stage of the TCA cycle. It is generally known to increase by aerobic exercise (Mellandy J. et al., Method of enzymatic analysis, Bergmeyer HU ed., 4: 1840-1843, 1974).

The CS enzyme activity was measured in the soleus muscle by modifying a known method (Srere PA, Method Enzymol, 13: 3-11, 1969). That is, the flounder muscle was extracted in the same manner as in Experimental Example <2-3> and added to 0.175M KCl buffer, and homogenized using a polytron homogenizer. In order to break the mitochondrial membrane in the homogenate, freezing and thawing were used three times as samples. In order to measure enzyme activity, 0.33 mL of 100 mM Tris-base buffer, 0.05 mL of 1 mM DTNB, 0.08 mL of 3 mL acetyl CoA, and 0.01 mL of the sample solution were mixed, followed by addition of 0.03 mL of 10 mM oxaloacetate and maintained at 30 ° C. Absorbance at 412 nm was measured using a spectrophotometer. Enzyme activity is expressed as μmol / g tissue / min.

As a result, the enzyme activity of the non-trained control group was significantly higher than that of the training control group, and the training group did not show a significant difference from the training control group (Table 9).

In the non-trained control group, the CS enzyme activity is high because the energy metabolism is relatively inferior to other groups because it is not trained for swimming. In the training group, the CS enzyme activity was not significantly different even though the exercise was performed for a relatively longer time than the other groups. Therefore, it can be seen from the above results that the composition according to the present invention has an effect of delaying muscle fatigue.

CS Enzyme Activity of Experimental Animals Forced to Swimming group Intramuscular CS enzyme activity (μmol / g tissue / min) Untrained control group 28.02 ± 2.62 Training control 22.77 ± 2.52 ⁺ Training experiment group 23.81 ± 1.04

+: Significant difference compared to non-trained control group (p <0.05)

<Example 2>

Preparation of a beverage composition for enhancing athletic performance according to the present invention

28.8 g of the composition prepared in Example 1, 0.16 g of citric acid, 12 g of high fructose, and 0.1 g of fragrance were mixed, and drinking water was added thereto so that the final volume was 120 mL. It was put into a pouch and sealed, and then heat sterilized at 100 ° C. for 30 minutes to prepare a beverage composition.

Experimental Example 3

Exercise performance enhancing effect of the beverage composition according to the present invention

The exercise performance enhancing effect of the human body according to the intake of the beverage composition prepared in Example 2 was investigated. The experimental results were expressed as mean ± standard deviation using the SPSS / PC 9.0 program. One-way ANOVA test was performed at the significance level of p <0.05 to analyze the statistical difference between the groups. Post-test was performed using Duncan's multiple range test.

<3-1> Selection of Subject and Intake Method of Drink Composition

In order to confirm the exercise performance enhancement effect of the beverage composition according to the present invention prepared in Example 2, the experiment was conducted on 10 male university students majoring in Y University physical education major without high blood pressure, cardiac function, etc. It was.

Ten subjects were asked to take the beverage composition of the present invention for 4 weeks at 180ml daily intake dose, and examined the change in exercise performance before and after ingestion.

The subjects were selected to have a physical ability to fully understand the purpose and purpose of the study and to perform the exercise presented in this experiment to the end. The physical characteristics of the subjects are shown in Table 10.

Physical Characteristics of Subjects Subject age Height (cm) Weight (kg) Body fat (%) O ₂ max (ml / kg / min) 85% O ₂ max (ml / kg / min) 10 people 20.0 ± 1.2 176.1 ± 5.4 69.3 ± 6.2 12.4 ± 2.9 55.9 ± 3.2 47.4 ± 2.8

<3-2> Measurement of Treadmill Running Exercise Performance Time

Treadmill running at 85% VO ₂ max intensity before and after the subjects selected in Experimental Example <3-1> to examine the effect of the beverage composition according to the present invention on exercise performance according to the present invention Exercise performance time was measured.

The start of the exercise was performed using the Bruce protocol, and the slope and speed were automatically increased every three minutes. When the exercise intensity was increased and the oxygen intake of each subject reached the estimated 85% O ₂ max level, the steady state was maintained by manually adjusting the slope and speed of the treadmill to maintain the target intensity. The exercise intensity was controlled by maintaining the steady state of oxygen intake while adjusting the inclination, and then the test was performed until the inclination was fixed at 6%, the speed was adjusted, and all out. During the exercise test, heart rate was measured at 5-minute intervals, and the rating of Perceived Exertion (RPE) was measured using the Borg 20 RPE scale.

As a result, the exercise performance time of the subject was found to be 30.03 ± 4.25 minutes before ingesting the beverage composition of the present invention and significantly increased to 33.25 ± 5.84 minutes after ingestion (p <0.05). Therefore, it was found that the exercise performance was increased by about 10.7% compared to before ingestion by ingesting the beverage composition of the present invention, and it was confirmed that the beverage composition according to the present invention has an effect of improving exercise performance time.

Experimental Example 4

Analysis of Blood Fatigue Factors According to Intake of Beverage Compositions of the Present Invention

Subjects were selected in the same manner as in Experimental Example <3-1>, and the beverage composition according to the present invention was ingested, followed by exercise in the same manner as in Experimental Example <3-2>, and before and after ingestion of the composition Blood was collected after exercise, and during recovery. Blood was collected from the actecubital vein each time using a 21 gauge needle. Lactic acid, ammonia, phosphoric acid and 5-HT concentration and pH were analyzed using the blood collected above.

<4-1> Changes in Lactic Acid Concentrations in Blood

In order to investigate the effect of ingestion of the beverage composition according to the present invention on blood lactic acid accumulation, the lactic acid concentration of blood collected from a subject was analyzed in the same manner as in Experimental Example <2-2>.

As a result of the experiment, blood lactate concentration showed the lowest level in the pre-exercise ballast regardless of whether the beverage composition of the present invention was ingested, and decreased after 30 minutes of recovery. However, the lactic acid concentration at 30 minutes of recovery did not recover to pre-exercise resting levels.

On the other hand, the concentration of lactic acid after ingestion of the beverage composition of the present invention was found to decrease by 10.4% after the end of exercise and about 20.4% after 30 minutes of recovery (Table 11). After the ingestion of the beverage composition of the present invention, despite the increase in exercise performance time compared to before ingestion, the fact that the concentration of lactic acid, a blood fatigue factor, was rather decreased. I knew it was.

Changes in Lactic Acid Concentration in Blood According to Intake of Beverage Composition of the Invention (Mean ± Standard Deviation) Drink composition intake Before exercise (mmol / L) After workout (mmol / L) Recovery 30 minutes (mmol / L) Before intake 1.37 ± 0.24 8.05 ± 2.52 2.94 ± 0.93 After ingestion 1.35 ± 0.24 7.21 ± 2.94 2.34 ± 0.56

<4-2> Changes in Blood Ammonia Levels

In order to investigate the effect of the intake of the beverage composition according to the present invention on the accumulation of blood ammonia, the ammonia concentration of blood collected from the subject was analyzed by the Green Cross Medical Foundation (Dongchon-dong, Gangseo-gu, Seoul).

As a result of the experiment, the blood ammonia concentration showed the lowest value before the exercise regardless of the ingestion of the beverage composition of the present invention, and showed the tendency to decrease 30 minutes after recovery after the highest value. Comparing before and after ingestion of the beverage composition according to the present invention, the ammonia concentration after the end of exercise and recovery 30 minutes after the intake was higher than before intake, but there was no statistically significant difference (Table 12). The activity of inhibiting the accumulation of blood ammonia, which is a fatigue factor, is due to the fact that there is no significant difference in blood ammonia concentration even after exercise time is increased compared to before ingestion of the beverage composition of the present invention. I could see that it has.

Changes in Blood Ammonia Concentration with Ingestion of the Beverage Composition of the Invention (Mean ± Standard Deviation) Drink composition intake Before exercise (μmol / L) After exercise (μmol / L) 30 minutes recovery (μmol / L) Before intake 77.71 ± 36.04 188.00 ± 57.00 95.85 ± 20.78 After ingestion 74.57 ± 30.27 205.42 ± 62.41 108.42 ± 26.63

<4-3> Changes in Inorganic Phosphate Levels in Blood

In order to investigate the effect of the intake of the beverage composition according to the present invention on the accumulation of inorganic phosphate in blood, the concentration of inorganic phosphate in blood collected from the subject was analyzed by the Green Cross Medical Foundation (Dungchon-dong, Gangseo-gu, Seoul).

As a result, the concentration of inorganic inorganic phosphate in blood showed the lowest level before exercise regardless of the ingestion of the beverage composition of the present invention. . On the other hand, after ingesting the beverage composition of the present invention was shown to have a higher inorganic phosphate concentration than before ingestion did not show a statistically significant difference at all times (Table 13). Despite the fact that the exercise composition time after ingestion of the beverage composition of the present invention was increased compared to before ingestion, there is no significant difference in the concentration of inorganic phosphoric acid in the blood. It was found that it has an activity.

Changes in Inorganic Phosphate Levels in Blood According to the Intake of the Beverage Composition of the Invention (Mean ± Standard Deviation) Drink composition intake Pre-Workout (mg / dL) After exercise (mg / dL) Recovery 30 minutes (mg / dL) Before intake 3.42 ± 0.56 4.91 ± 0.63 3.48 ± 0.58 After ingestion 3.50 ± 0.41 5.02 ± 0.81 3.77 ± 0.52

<4-4> Change in pH of blood

In order to investigate the effect of ingestion of the beverage composition according to the present invention on the pH of blood, the pH of blood collected from the subject was measured by a pH meter.

As a result, blood pH gradually decreased with increasing exercise time regardless of ingestion of the beverage composition of the present invention, showed the lowest level after the end of the exercise, and increased to 30 minutes after recovery, showing a tendency to recover to almost stable level. Changes in blood pH according to the intake of the beverage composition of the present invention did not show a significant difference at all times (Table 14).

Changes in pH of blood according to the intake of the beverage composition of the present invention (mean ± standard deviation) Whether to drink beverage composition Before exercise After exercise 30 minutes recovery Before intake 7.31 ± 0.12 7.04 ± 0.23 7.26 ± 0.18 After ingestion 7.34 ± 0.21 7.07 ± 0.31 7.30 ± 0.27

<4-5> Changes in 5-HT Levels in Blood

In order to investigate the effect of the ingestion of the beverage composition according to the present invention on the concentration of 5-HT (serotonin) in the blood, 5-HT concentration of blood collected from the subject was analyzed by the Green Cross Medical Foundation.

As a result of experiment, the concentration of 5-HT in blood gradually increased with increasing exercise time regardless of ingestion of the beverage composition of the present invention and showed the highest value after the end of exercise. The trend was shown. In addition, the blood 5-HT concentration after ingestion of the beverage composition of the present invention was shown to be higher than before intake, but there was no statistically significant difference (Table 15).

Accumulation of 5-HT in the blood composition of the beverage composition of the present invention is due to the fact that there is no significant difference in the blood 5-HT concentration despite the increase in exercise performance time after ingestion of the beverage composition of the present invention. It can be seen that it has an activity to inhibit.

Change in the concentration of 5-HT in the blood according to the intake of the beverage composition of the present invention (mean ± standard deviation) Whether to drink beverage composition Pre-workout After exercise 30 minutes recovery Before intake 14.55 ± 2.10 17.29 ± 2.53 14.01 ± 2.81 After ingestion 14.03 ± 2.44 18.35 ± 2.83 15.07 ± 2.95

The composition according to the present invention is useful for enhancing physical fitness and athletic ability of the athlete because it has an effect of improving exercise performance time when prolonged ingestion and inhibiting accumulation of blood fatigue substances during exercise.

Claims (4)

Exercise consisting of 75 to 85% by weight of medium chain triglycerides, 0.5 to 1.0% by weight of octacosanol, 5 to 15% by weight of red ginseng extract, 1 to 5% by weight of Bokbunja water extract, and 1 to 5% by weight of Ogapi water extract Performance enhancing composition. According to claim 1, 1 to 5 parts by weight of the collagen extract or powder, 1 to 5 parts by weight of taurine, 0.1 to 1.0 parts by weight of phosphatidylcholine, 1.5 to 2.5 parts by weight of glutamine, L- based on 100 parts by weight of the composition of claim 1 1.5 to 2.5 parts by weight of arginine and 1.5 to 2.5 parts by weight of L-carnitine further comprises a composition for enhancing athletic performance. A beverage comprising the composition of claim 1. The composition according to claim 3, wherein the composition 20-25% (w / v), citric acid 0.1-0.2% (w / v), high fructose 5-15% (w / v), fragrance 0.05-0.1% (w / v) ) And the remaining amount of drinking water.