RU2423891C1 - Actoprotection action beverage - Google Patents

Abstract

FIELD: food industry. ^ SUBSTANCE: beverage contains 1.0-1.5 kg of citric acid, 0.150-0.177 kg of sodium benzoate, 90-200 kg of a flavour additive and carbonated or non-carbonated water up to 100 decalitres of the beverage. Apart from the above components, the beverage contains (per 100 decalitres) a mixture of active components including 15-25 kg of natural honey, 90 kg of rosehips syrup and 0.15-0.25 kg of refined amberat extract dissolved in water; the ratio of amberat extract to water 1:8. ^ EFFECT: beverage promotes physical stamina, biological activity of the organism, additionally it effectively mitigates fatigue, increases muscle strength, raises pain threshold; it has high organoleptic properties; the said beverage may be consumed by any people groups regardless of age and state of health; the beverage has a long storage life; sediment formation in it is excluded. ^ 8 tbl, 4 ex

Description

The invention relates to the field of non-alcoholic industry and can be used in the production of a drink having an actoprotective effect and intended, in particular, to increase the physical endurance of athletes.

Modern sport is characterized by an extremely high level of physical and mental stress on the body. Adaptation to the action of intense training and competitive loads in modern sports is achieved through the use of a range of measures, including pedagogical, psychological and biomedical methods. At present, the level of modern training of athletes involves a further increase in body loads. In this regard, expanding the boundaries of athletes' physical performance, increasing its potential, preventing overwork and accelerating recovery processes is an urgent problem, since sports training, which is the main method of increasing physical endurance, has its limits. This is especially important when training high-class athletes, since the physical and mental stress that accompanies the training and participation of athletes in important competitions often leads to a violation of the functional capabilities of the body. In this regard, the optimization of the training process by introducing new highly effective products from raw materials of natural origin, which can ensure the adaptation of the body to increasing loads and allow ensuring the achievement of higher sports results, remains a fundamental task.

The prior art drinks are known whose action is aimed at increasing physical endurance.

So, from the description of the patent of the Russian Federation No. 2023401 (IPC 5 A23L 2/00, published November 30, 1994), a concentrate of a tonic drink that increases physical endurance is known, which contains sugar, citric acid, water-alcohol infusion of Eleutherococcus, and water, characterized in that it additionally contains grape juice and water-alcohol infusions of pharmacy chamomile, medicinal sage and spikelet lavender in the following ratio of components, kg / 1000 kg of concentrate:

Sugar 550-600 Lemon acid 10.3-11.7 The grape juice 120.0-150.0 Water-alcohol infusion of Eleutherococcus 3,5-4,9 Water-alcohol infusion of chamomile pharmacy 14.9-16.9 Water-alcohol infusion of sage 6.3-8.6 Water-alcohol infusion of spikelet lavender 3.0-4.9 Water rest.

Also from the description of the patent of the Russian Federation No. 2233168 (IPC 7 A61K 35/78, published July 27, 2004), the composition of ingredients for producing an elixir containing dry chokeberry fruits, rosehips, linden flowers, bitter wormwood grass, chaga, pepper is known black, nutmeg, nettle leaves, prune fruits, propolis, aloe juice or aloe leaves are tree-like dry, bee honey and ethyl alcohol 40%, characterized in that it additionally contains ginseng roots, purple echinacea grass, oregano grass, hawthorn fruit, rhizomes frankincense, St. John's wort grass, wines dried fences and sugar color or sugar in the following ratios of ingredients, g:

Ginseng Roots 0.1-0.6 Echinacea purpurea herb 1.5-30.0 Propolis 0.25-2.5 Dry aloe tree leaves or 0.25-2.5 Aloe juice 15.0-150.0 Chaga 0.5-10.0 Rhizomes of Badiana 0.5-10.0 St. John's wort grass 0.5-10.0 Hawthorn fruit 0.5-10.0 Dry chokeberry fruits (chokeberry) 2.5-50.0 Dog-rose fruit 5.0-100.0 Linden flowers 1,0-20,0 Wormwood grass 0.025-0.5 Nettle leaves 1,0-20,0 Honey bee 2.5-50.0 Oregano grass 0.05-1.0 Dried grapes 2.0-40.0 Nutmeg 0.25-5.0 Prune fruits 5.0-100.0 Black pepper 0.05-1.0 Sugar 150.0 Sugar color 225 Ethyl alcohol 40% to receive 1000 ml of elixir.

In addition, from the patent of the Russian Federation No. 2060015 (IPC 6 A23L 2/00, published May 20, 1996) a tonic drink is known containing sugar, citric acid, tincture of hawthorn fruit, carbon dioxide and water, characterized in that it additionally contains tincture of lemongrass stems , tincture of badan leaves, sodium benzoate, glutamic acid and sodium humate in the following ratio of ingredients, l / 100 gave the drink:

Infusion of hawthorn fruit 40 50 Infusion of lemongrass stems 3 4 Infusion of frankincense leaves 8 10 Humate sodium 12

as well as kg

Sugar 55 60 Sodium benzoate 0.175 0.177 Lemon acid 0.45 0.50 Glutamic acid 0.06 0.10 Carbon dioxide 3-4 Water rest.

The disadvantages of the known drinks is that they contain sugar, which leads to high calorie drinks. In addition, the use of alcohol-containing components (tinctures) can cause unwanted effects in the form of increased irritability, irritation of the gastric mucosa, and insomnia. The intake of such drinks is contraindicated for athletes, people under the age of 18 years, pregnant and lactating women, elderly people, as well as those suffering from increased nervous irritability, insomnia, impaired cardiovascular system, and hypertension.

Based on the foregoing, the task was set to develop a recipe for a non-alcoholic vitamin drink that increases physical endurance, mass consumption based on components widely used in traditional medicine of many peoples of the world, such as mummy extract, rosehip syrup and honey.

The technical result of the present invention is to obtain a drink, which increases physical endurance, biological activity of the body, effectively relieves fatigue, increases muscle strength, increases the threshold of pain sensitivity, and also has high organoleptic properties that can be consumed by any group, regardless of age and state of health. In addition, the claimed technical solution makes it possible to obtain drinks with different taste qualities. Also the technical result of the solution is to obtain a drink with a long shelf life and the exclusion of sediment.

The claimed technical result is achieved through the use of a drink that increases physical endurance, which contains citric acid, carbonated or non-carbonated water, a flavoring additive, sodium benzoate and a mixture of active components, consisting of natural honey, a water-soluble extract of mummy and rosehip syrup, in the following ratio of components to 100 gave a drink:

natural honey 15-25 kg rosehip syrup - 90 kg lemon acid 1.0-1.5 kg purified mumiye extract dissolved in water  0.15-0.25 kg while the ratio of mummy extract to water is 1: 8 sodium benzoate 0.150-0.177 kg flavoring additive 90-200 kg water rest.

As a flavoring additive, the drink may contain berry, and / or fruit, and / or herbal syrup, in particular syrup of rose hips, raspberries, cherries, milk thistle, tarragon, sea buckthorn, lemon, lime, as well as tea extract, matcha tea, green tea .

As an extract of the mummy, used as one of the components of the detoxifying mixture, a dry mummy extract of high purity can be used.

Mumiye dry extract contains micro and macro elements (cobalt, nickel, copper, zinc, manganese, chromium, iron, sodium, potassium, magnesium), humic acids, polyphenolic compounds, amino acids, steroids, phospholipids.

Immediately before the preparation of the drink, the dry mummy extract is dissolved in water in a ratio of 1: 8 (1 part mummy and 8 parts water).

Thanks to the use of the purified mummy extract, its 100% solubility in water is achieved in the selected ratio without the formation of a precipitate during its further storage.

A mixture of active components in the claimed ratio has a tonic strengthening effect. It activates the defenses, increases the nonspecific resistance of the body by a harmful factor, increases mental and physical performance, endurance in extreme conditions - with sharp temperature fluctuations, oxygen starvation, radiation exposure, heavy metal poisoning, stimulates the immune defense and resistance to infections, provides an antioxidant effect.

The listed properties of the mixture are achieved due to the content of mummy, honey and rosehip syrup in it.

Mummy is used as a restorative and tonic, replacing multivitamins and trace elements. Mummy removes the feeling of fatigue and acts on the human body tonic, contribute to the addition and restoration of lost strength, energy. The positive cardiotonic effect of the mummy is expressed in a beneficial effect on the work of the heart with high energy costs. Mummies also stimulate mental activity.

Mumiye has almost no side effects, except in cases of individual intolerance or its use in excessive doses.

The use of rosehip syrup mixed with mummy extract dissolved in water leads to stabilization of the mummy dissolved in water due to the content of natural ascorbic acid in the syrup.

Also, due to the content of vitamins B2, C and P, flavonoids, carotenoids, tannins, pectins in rosehip syrup, it has a general strengthening effect on the body, stimulates its resistance, enhances tissue regeneration, reduces vascular permeability, takes part in carbohydrate and mineral metabolism, It has anti-inflammatory properties.

In addition, the ascorbic acid and pectins contained in the rosehip syrup, as well as citric acid, are antioxidants.

Honey combined with mummy and citric acid gives the drink a pleasant taste and aroma. Honey also neutralizes the bitter taste of mummies, enriches the drink with organic acids, vitamins and trace elements. In addition, honey is an invert sugar, which allows a long time to preserve easily oxidized substances and, accordingly, provides a long shelf life of the drink.

Glucose, contained in large quantities in honey, quickly enters the bloodstream, participates in oxidative processes and provides energy to muscles, the brain and other vital organs.

So, a mixture of natural honey and mummy contains (t / 100 g): water 23.8, protein 0.77, fat 0.23, carbohydrates 74.0, ash 0.26, the energy value of the product is 300 kcal.

The protein content in the mixture and the high calorie content of honey in the presence of this combination of proteins contributes to the easy digestibility of the drink, creates the optimal concentration of the most important proteins and vitamins in the body, and helps digestion and assimilation of food.

The patented composition of the drink accelerates the recovery process with depleting physical activity.

The process of preparing a patentable beverage includes the following main technological stages:

- water treatment;

- preparation of an aqueous solution of mummy;

- preparation of blend syrup;

- bottling, clogging;

- storage and transportation.

Water treatment is carried out in accordance with the requirements of GOST R 51232-98.

Mumiye extract (Shilajit) in the amount of 0.15-0.25 kg is dissolved with stirring in water in a ratio of 1: 8. The obtained aqueous mummy solution is added to the blending tank and 100 l of water are added. After that, honey is added to the blending tank with constant stirring and the calculated amount of rosehip syrup is added. After that, pre-dissolved citric acid and sodium benzoate are introduced into the blending tank. By adding water, the volume of the drink is adjusted to 1000 l. All components are mixed for 15-20 minutes, after which they are left for 30 minutes for better assimilation of the aromatic substances of the blend, suppression of the growth of microorganisms by the preservative, and also for the possible isolation of insoluble components and colloidal particles in the sediment. After checking the physico-chemical and organoleptic characteristics, the drink is filtered and sent for bottling.

The invention is further illustrated by the following examples of specific beverage compositions, kg per 100 dal.

Example 1

Natural honey 15 kg lemon acid 1,0 kg Mumiye extract dissolved in water 0/15 (the ratio of mummy extract to water was 1: 8) sodium benzoate 0.150 kg rosehip syrup 90 kg flavoring additive (tarragon syrup) 90 kg sparkling water rest.

Example 2

natural honey 25 kg lemon acid 1,5 kg Mumiye extract dissolved in water 0/25 (ratio of mummy extract to water - 1: 8) sodium benzoate 0.177 rosehip syrup 115 kg cherry syrup 100 kg sparkling water rest.

Example 3

natural honey 20 kg lemon acid 1.3 kg Mumiye extract dissolved in water 0/25 (ratio of mummy extract to water 1: 8) sodium benzoate 0.150 kg rosehip syrup 100 kg tarragon syrup 120 kg still water rest.

Example 4

natural honey 18 kg lemon acid 1.1 kg Mumiye extract dissolved in water 0.18 (ratio of mummy extract to water 1: 8) benzoatrium 0.160 kg rosehip syrup 50 kg cherry syrup 100 kg still water rest.

Due to the selected composition of natural components, the resulting drink can be recommended for consumption daily by any age groups, which was proved by preclinical trials of drinks prepared in accordance with examples carried out by the Institute of General and Experimental Biology SB RAS.

As a result of research, the influence of “Drink” on the general physical endurance of animals was determined.

The experiments were conducted on mice of the CBA line of both sexes weighing 18-20 g. To determine the total physical endurance, we used the generally accepted method of swimming in a pool with a water temperature of 20.0 ± 0.5 ° C with a load of 5% of the animal’s body weight. The drink was administered intragastrically in volumes of 0.5; 1.0 and 2.0 ml / 100 g once for 1 hour before testing, and also repeatedly for 7 days in a volume of 1.0 ml / 100 g once a day 30 minutes before meals. The animals of the control group were intragastrically injected with a similar volume of distilled water. The duration of swimming of the animals to complete fatigue was evaluated, the criterion of which was a 10-second immersion in water.

The data obtained were processed by statistically generally accepted methods for a small sample with the determination of the arithmetic mean value (M) and the arithmetic mean error (m). To assess the significance of differences, Student's test was used. Differences were considered significant with a probability of 95% (P <0.05). The data obtained are shown in table 1.

Table 1 The effect of "Drink" on the duration of swimming white mice Groups of animals Volume ml / 100 g Duration of swimming, min with a single injection (n = 10) with repeated administration (n = 10) Control - 14/6 ± 1.32 17.5 ± 1.55 Drink 0/5 15.5 ± 1.40 - Drink 1,0 19/3 ± 1.70 28.8 ± 2.40 Drink 2.0 21.2 ± 1.48

As follows from the data given in table 1, a single injection of a drink in a volume of 0.5 ml / 100 g did not have a significant effect on the duration of swimming of animals, whereas when used in a volume of 1.0 ml / 100 g, an increase in overall physical endurance was noted 32% compared with data from animals in the control group (P <0.05). With an increase in the volume of injected drink, an increase in its actoprotective activity was noted. So, with the introduction of the test drug in a volume of 2.0 ml / 100 g, the duration of swimming of the animals of the experimental group increased on average by 45% compared with that of mice of the control group. However, the swimming time of rats treated with a drink in a volume of 2.0 ml / 100 g was not statistically different from that in animals that were administered in a volume of 1.0 ml / kg. Based on this, in further experiments, the volume of “Drink” corresponding to 1.0 ml / 100 g was used as the experimental therapeutic.

Seven-day preventive administration of the drink in a volume of 1.0 ml / 100 g was accompanied by a more significant increase in overall physical endurance, as evidenced by an increase in the duration of swimming of animals of the experimental group by an average of 65% compared with the performance of animals in the control group.

Thus, the data obtained indicate that the drink has a stimulating effect on overall physical endurance, characterized by the predominance of aerobic processes of tissue energy supply. It was found that the test agent possesses the most pronounced actoprotective activity when administered in volumes of 1.0 and 2.0 ml / kg; its effectiveness increases with repeated administration.

In addition, we determined the effect of the drink on high-speed physical endurance against the background of rest relative functional rest.

The experiments were carried out on mice of a CBA line of both sexes weighing 18-20 g. The effect of this agent on high-speed physical endurance was determined by the duration of the animals running on a 10-track treadmill at a speed of 40 m / min treadmill cloth (Savichev, 1971). "Drink" was administered intragastrically in a volume of 1.0 ml / 100 g once 1 hour before testing, and also repeatedly for 7 days in the indicated volume 1 time per day for 30 minutes before 10 meals. The animals of the control group were intragastrically injected with a similar volume of distilled water. The duration of the run of the animals to complete fatigue was evaluated, the criterion of which was the 10-second finding of the animal on the treadmill's electric grid under a voltage of 70 V.

table 2 The effect of "Drink" on the duration of running white mice Groups of animals Running Duration, s with a single injection (n = 10) with repeated administration (n = 10) Control 210.3 ± 15.6 218.5 ± 23.6 Drink 226.5 ± 19.3 296.0 ± 29.4

As follows from the data given in table 2, a single injection of the drink in the indicated volume did not have a significant effect on the speed physical endurance of white mice, the running time of the animals of the experimental group did not statistically differ from that in the animals of the control group. It was found that repeated administration of the test drug for 7 days was accompanied by a significant increase in speed endurance, as evidenced by an increase in the running time of animals of the experimental group by 35% compared with the data of mice in the control group.

Thus, the data obtained indicate that the drink in the volume of 1.0 ml / 100 g increases the speed physical endurance, characterized by the predominance of anaerobic processes in the tissues, only with repeated administration.

Next, we determined the effect of the drink on high-speed physical endurance against the background of dosed physical activity.

The experiments were conducted on mice of the CBA line of both sexes weighing 18-20 g. Preliminary physical activity was carried out in accordance with the methodological recommendations using forced run of animals in a treadmill at a speed of the tape of 28 m / min for 5 minutes. Animals that received preliminary physical activity were divided into 2 subgroups. In the first subgroup, the mice were subjected to a single physical activity for 5 minutes. Following this, the animals were intragastrically injected with a drink in a volume of 1.0 ml / 100 g, and after 1 hour, fast physical endurance was determined. Animals of the second subgroup received daily dosed physical activity for 7 days. On the first day, the duration of the run was 5 minutes; on the following days, the duration of the load was sequentially increased by 1 minute. Immediately after the load, the mice were injected with the test drug in a volume of 1.0 ml / 100 g. Animals in the control group were injected with distilled water in the same volume. On day 7, 1 h after the last injection of the drink, speed endurance was determined. The data obtained are shown in table 3.

Table 3. The effect of the drink on the duration of running of white mice with its introduction against the background of preliminary physical exertion Groups of animals Running Duration, s at a single load (n = 10) at 7 days load (n = 10) Intact 248.5 ± 23.4 * 248.5 ± 23.4 Control 153.3 ± 14.5 294.0 ± 26.5 Drink 235.7 ± 22.6 * 496.0 ± 28.2 *

As follows from the data given in table 3, a single dosed load causes a decrease in performance in mice of the control group, as evidenced by a significant decrease in the duration of running of animals of this group (on average by 40% compared with data from intact mice). A single introduction of "Drink" in a volume of 1.0 ml / 100 g against a single dosed load exerted a protective effect, preventing a decrease in the physical performance of the mice of the experimental group. So, in mice that received the test drug, the duration of running did not statistically differ from that in intact animals.

In the second series of experiments, it was found that in animals of the control group subjected to seven-day dosed physical activity, there is a tendency to increase the duration of running in a treadmill (P> 0.05). Moreover, the daily administration of the test drink against the background of dosed loads had a pronounced stimulating effect on the high-speed physical endurance of the mice of the experimental group, as evidenced by an increase in the running time of mice of the experimental group by an average of 70% compared with the data of the animals of the control group.

Thus, the obtained data indicate that the “Drink” in the volume of 1.0 ml / 100 g has an act protective effect, preventing the development of fatigue after a single dosed physical load and maintaining the physical performance of mice at the level of indicators of intact animals. The test agent exerts a more pronounced actoprotective effect with repeated administration against the background of daily dosed physical activity.

Then examined the effect of the drink on high-speed physical endurance against a background of inactivity.

The experiments were carried out on mice of the CBA line of both sexes weighing 18-20 g. Hypodynamia was created by hanging animals from the neck fold for 3 days for 18 hours each day. The animals of the experimental group 1 hour before suspension were injected intragastrically with a drink of 1.0 ml / 100 g. The mice of the control group received an equivalent volume of distilled water under similar conditions. In the intervals between experimental suspensions, the animals received proper nutrition. The effect of the test agent on physical endurance was evaluated by the duration of the animals running in the treadmill at a speed of movement of the canvas of 28 m / min (Krendal et al., 1981). The data obtained are shown in table 4.

Table 4 The effect of "Drink" on the duration of running of white mice on the background of inactivity Groups of animals Running time, s (n = 10) Intact 278.6 ± 25.5 * Control 156.5 ± 22.8 Drink 312.0 ± 19.2 *

The data shown in table 4 indicate that the physical performance of animals after three-day inactivity is reduced by 45% compared with indicators of intact mice. At the same time, daily administration of the drink against the background of physical inactivity contributed to maintaining the physical endurance of the animals of the experimental group at the level of indicators of intact animals, and in comparison with the data of the mice of the control group, the working capacity in animals receiving the test drug was doubled.

Thus, the data obtained indicate that the introduction of a drink in a volume of 1.0 ml / 100 g against a background of inactivity has a protective effect, preventing a decrease in the physical performance of animals that develops as a result of inactivity.

The effect of the drink on strength endurance was also determined.

The experiments were carried out on mice of the CBA line of both sexes weighing 18-20 g. The influence of the test product on strength endurance was determined by comparing the time of the animals hanging on the pole from the beginning of their retention until the moment of fall. The drink was administered intragastrically in a volume of 1.0 ml / 100 g once 1 hour before testing, and also repeatedly for 7 days in the indicated volume 1 time per day 30 minutes before a meal. The animals of the control group were intragastrically injected with a similar volume of distilled water. The data obtained are shown in table 5.

Table 5 The effect of the drink on the duration of the hanging white mice Groups The duration of the hang, min with a single injection (n = 8) with repeated administration (n = 8) Control 12.5 ± 1.42 13.0 ± 1.62 Drink 16.3 ± 1.35 * 20.4 ± 1.45 *

As follows from the data given in table 5, with a single injection of "Drink" in a volume of 1.0 ml / 100 g, the hanging time of mice of the experimental group increases by 30% compared with that in animals of the control group. Repeated administration of the test agent is accompanied by a more pronounced increase in the animal's endurance of strength, as evidenced by an increase in the duration of the hanging mice of the experimental group by an average of 60% compared with the data of animals in the control group.

Thus, the data obtained indicate that the "Drink" in a volume of 1 ml / 100 g helps to increase the strength endurance of animals.

Then, the effect of the drink on biochemical parameters during intense physical exertion was investigated.

The experiments were carried out on Wistar rats of both sexes weighing 160-180 g. Animals of the experimental group were intragastrically injected with a drink in a volume of 1.0 ml / 100 g once a day for 21 days. Rats in the control group received an equivolume amount of distilled water. On the 21st day of the experiment, the animals of the control and experimental groups were divided into two subgroups. The animals of the first subgroups swam with a 7% load until fatigue, the criterion of which was the first immersion of the animal under water. After this, the rats were decapitated and the concentration of malondialdehyde (MDA) in the blood serum and in the skeletal muscle homogenate was determined; serum concentration of diene conjugates; serum sulfhydryl groups; serum catalase activity; ATP concentration in skeletal muscle homogenate; serum lactic and pyruvic acid content. Animals of the second subgroup after physical activity received a single drink in the indicated volume and after a 3-hour rest, they again determined the overall physical endurance. The data obtained are shown in tables 6-8.

Table 6 The effect of the drink on general physical endurance and indicators of the energy status of the organism of white rats on the background of intense physical activity (IFN) Indicators Groups of animals Intact (n = 12) Control (IFN + H ₂ O) (n = 10) Experienced (IFN + Drink) (n = 10) ATP in the skeletal muscle, units OP 0.55 ± 0.03 0.28 ± 0.01 0.37 ± 0.03 * ATP in the myocardium, units OP 0.29 ± 0.04 0.18 ± 0.01 0.23 ± 0.01 * Glycogen in the liver, g% 1012.0 ± 76.8 778.1 ± 52.7 975.0 ± 43.2 * Pyruvic acid, mmol / L 3.2 ± 0.25 3.9 ± 0.26 3.1 ± 0.34 * Lactic acid, mmol / l 48.5 ± 3.63 102.0 ± 4.50 65.0 ± 5.52 * MK / PVC 15.1 ± 1.33 26.1 ± 2.54 20.9 ± 1.82 *

As follows from the data given in table 6, intense physical activity is accompanied by a significant decrease in the concentration of ATP in skeletal and cardiac muscles and an increase in the level of lactic acid in skeletal muscles, which is the main limiting factor of physical performance. In addition, against the background of intense physical activity, depletion of carbohydrate reserves was observed, as indicated by a decrease in the concentration of glycogen in the liver. Preventive repeated administration of the drink had a pronounced actoprotective effect, as evidenced by the increase in the overall physical performance of the animals of the experimental group: the duration of swimming increased by an average of 35% compared with the data of the animals of the control group. An analysis of energy metabolism indicates that the increase in animals' physical endurance under the influence of the drug is due to the activation of ATP resynthesis: its content in skeletal and cardiac muscles was on average 30% higher compared to similar indicators in animals of the control group. Along with this, under the influence of the test agent, a significant decrease in the concentration of lactic acid (by 36%) was observed, accompanied by a decrease in the lactate / pyruvate coefficient by an average of 20% compared with the control.

Table 7 The effect of “Drink” on the intensity of LPO processes and the state of the antioxidant system of the organism of white rats against the background of intense physical activity Indicators Groups of animals Intact (n = 10) Control (IFN + H ₂ O) (n = 12) Experienced (IFN + Drink) (n = 12) MDA in serum, μmol / ml min 37.2 ± 2.36 62.5 ± 3.85 52.9 ± 3.17 * MDA in skeletal muscle, nmol / g 27.2 ± 2.36 58.7 ± 4.18 38.1 ± 2.67 * MDA in the myocardium, nmol / g 38.0 ± 2.67 65.4 ± 3.36 45.2 ± 2.13 * SH-groups, mmol / l 34.3 ± 2.42 18.2 ± 1.83 25.8 ± 1.52 * Catalase, mkat / l 24.7 ± 1.15 18.3 ± 1.56 22.6 ± 1.38 *

As can be seen from the data given in table 7, in animals against the background of maximum physical activity, a significant increase in the level of MDA in tissues is noted, along with a decrease in the concentration of sulfhydryl groups and serum catalase activity, which indicates the induction of lipid peroxidation and inhibition of the activity of the endogenous antioxidant system organism as a result of swimming to complete exhaustion. Prophylactic administration of the drug in the indicated volume against the background of physical activity was accompanied by less pronounced changes in the indicators of free radical oxidation and the parameters of the antioxidant system. Thus, the concentration of MDA in the blood serum, homogenates of the skeletal and cardiac muscles of the animals of the experimental group was 25, 35 and 29% less, respectively, compared with the same rats of the control group. Along with this, against the background of the administration of the test agent in the blood serum of animals of the experimental group, an increase in the concentration of sulfhydryl groups by an average of 40%, as well as an increase in the activity of catalase by 23% compared with the control, which indicates the activation of the antioxidant system of the body under the influence of the tested means .

Table 8 The effect of "Drink" on the duration of repeated swimming of white rats after 3 hours of rest Groups Duration of swimming, min Control (IFN + H ₂ O) (n = 12) 5.1 ± 0.41 Experienced (IFN + Drink) (n = 10) 6.9 ± 0.16 *

As follows from the data given in table 8, the introduction of the test agent helps to accelerate the process of restoring physical performance after intense physical activity, as evidenced by the data of repeated testing: the physical performance of animals of the experimental group was 35% higher than that of rats in the control group.

Thus, the data obtained indicate that the basis of the actoprotective activity of the drink is a whole series of functional and metabolic changes at various levels of biological organization. In particular, it was found that under the influence of the indicated agent the content of macroergic compounds in the cardiac and skeletal muscles increases. The activation of the synthesis of macroergic compounds is combined with a distinct increase in the glycogen content in the liver, which indicates an increase in carbohydrate reserves, which are known to be the main oxidation substrate during intense physical exertion. The revealed decrease in the concentration of lactic acid in the muscles indicates a decrease in the severity of metabolic acidosis. It was also established that under the influence of the test agent the intensity of LPO processes decreases and the activity of the endogenous antioxidant system increases.

Thus, experimental studies of a drink containing mumiye extract, rosehip syrup and honey as active substances have shown that it has a pronounced actoprotective effect, increasing the overall, speed and strength physical performance of animals. The actoprotective effect of the drink is more pronounced on the altered reactive background of the body. So, it has been established that its administration against the background of dosed physical exertion increases the period of development of fatigue, stimulates the processes of restoration of working capacity, which is accompanied by an increase in the endurance of animals during repeated physical exertion. It has been established that the mechanism of the actoprotective effect of the drug is associated with optimization of the energy status of the body, a decrease in the severity of metabolic acidosis, activation of synthetic processes and inhibition of free radical oxidation.

Claims (5)

1. A drink of act-protective action, characterized in that it contains citric acid, carbonated or non-carbonated water, a flavoring additive, sodium benzoate and a mixture of active ingredients, consisting of honey, a natural, water-soluble extract of mummy and rosehip syrup, in the following ratio of the starting components per 100 gave a drink :
natural honey 15-25 kg rosehip syrup 90 kg lemon acid 1.0-1.5 kg purified mumiye extract dissolved in water 0.15-0.25 kg while the ratio of mummy extract to water is 1: 8 sodium benzoate 0.150-0.177 kg flavoring additive 90-200 kg water rest 2. The drink according to claim 1, characterized in that as a flavoring additive used berry, and / or fruit, and / or herbal syrup. 3. The drink according to claim 2, characterized in that the raspberry and / or lingonberry and / or blueberry and / or cranberry and / or cherry syrup is used as berry syrup. 4. The drink according to claim 2, characterized in that tarragon syrup is used as a herbal syrup. 5. The drink according to claim 1, characterized in that as the extract of the mummy use dry powder of the extract of the mummy.