CN114085764A - Movable microbial fermentation system and method for environmental remediation - Google Patents
Movable microbial fermentation system and method for environmental remediation Download PDFInfo
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Abstract
The invention belongs to the field of environmental bioengineering, and particularly relates to a movable microbial fermentation system and a movable microbial fermentation method for environmental remediation. The fermentation box is provided with a unique two-chamber design, the fermentation amount of the microbial inoculum can be effectively controlled according to the actual condition of the process, and when the usage amount of the microbial inoculum is not large each time and the field does not have storage conditions, unnecessary waste is avoided, and the requirement of the current double-carbon target is met. In addition, the cost is low, the fermentation process is convenient to operate, the sterilization, cooling and culture integrated operation of the fermented materials is not subjected to external procedures, and the risk of contamination is controlled to be minimum. Meanwhile, the system is designed to be integrally movable, so that the transportation and the field use are convenient, particularly, the production and transportation cost of the microbial preparation is greatly reduced aiming at environmental microbial remediation projects with limited remote areas and field conditions, the high-activity microbial preparation can be provided according to the requirements of the projects at any time, and the success rate of the projects is ensured.
Description
Technical Field
The invention belongs to the field of environmental bioengineering, and particularly relates to a movable microbial fermentation system and a movable microbial fermentation method for environmental remediation.
Background
Under the requirement of a double-carbon overall target, the microbial remediation technology is paid more and more attention and is applied to the field of environmental remediation due to the characteristics of low remediation cost, good safety, no secondary pollution and the like, engineering microbial remediation generally needs to continuously input a large amount of exogenous microbial liquids, enzymes, nitrogen, phosphorus, inorganic salts and the like into a polluted environment, and particularly, microbial liquids are used in large quantities and need to be continuously supplemented. The bacteria liquid used at the present stage is mostly processed in different places, so that the problems that the production period is long, the production, fermentation, transportation and storage costs are high, the adjustment cannot be carried out in time according to the field process condition and the like exist, the two poles of equipment capable of producing the strains on the field are seriously differentiated, one is that the equipment price is too high, and the loss of the field fermentation equipment is large; one is simple and crude equipment, the fermentation process is not controlled, and the finished product yield of the microbial inoculum is low.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a movable microbial fermentation system and a movable microbial fermentation method for environmental remediation, and aims to solve the problems that in the prior art, bacterial liquid is processed in different places, the production period is long, the production fermentation and transportation and storage costs are high, and the bacterial liquid cannot be adjusted in time according to the field process conditions; and the equipment price for producing the strain on site is too high, the loss of the fermentation equipment on site is large, or the equipment is simple and crude, the fermentation process is not controlled, and the yield of the finished microbial inoculum is low.
The invention provides a movable microbial fermentation system for environmental remediation, which has the following specific technical scheme:
a movable microbial fermentation system for environmental remediation comprises a fermentation box, a water supply system, a heating and sterilizing system, a condensing system, a fermentation and gas supply system and a control system; the fermentation box is provided with two fermentation chambers, and each fermentation chamber is provided with a coil pipe and a stirring mechanism; the water supply system supplies water into the fermentation chamber; the heating sterilization system is used for introducing high-temperature steam through the coil pipe to perform sterilization operation on the fermentation chamber; the condensing system is used for introducing condensed water through the coil pipe to cool the fermentation chamber; the fermentation air supply system is used for supplying oxygen to the fermentation chamber, the control system controls one or two fermentation chambers according to the fermentation amount, the water supply operation, the sterilization operation, the cooling operation and the oxygen supply operation are performed, and the stirring mechanism is controlled to start and stop.
In some embodiments, a partition board is arranged in the fermentation box, the partition board divides the fermentation box into two fermentation chambers, an air outlet is formed in the top of the fermentation box, and the tops of the two fermentation chambers are communicated with each other and are both communicated with the air outlet.
In some embodiments, two feeding ports are formed at the top of the fermentation box, and the two feeding ports are respectively aligned with the tops of the two fermentation chambers; two discharge ports are formed in the bottom of the fermentation box and are respectively aligned with the bottoms of the two fermentation chambers, and the discharge ports are configured to discharge products from the fermentation chambers or take out samples; two inoculation ports are further formed in the top of the fermentation box, and the two inoculation ports are respectively aligned to the tops of the two fermentation chambers.
In some embodiments, the heat sterilization system includes a steam generator, a steam inlet line, and a steam outlet line, the steam generator being in communication with the top port of each of the coils through the steam inlet line, and the bottom port of each of the coils being in communication with the steam outlet line.
In some embodiments, the condensing system includes a condensate inlet line and a condensate outlet line connected to a source of tap water, the condensate inlet line being in communication with the bottom port of each of the coils, respectively, and the condensate outlet line being in communication with the top port of each of the coils, respectively.
In some embodiments, the water supply system comprises a water inlet line connected to a source of tap water, the water inlet line communicating with the bottom of each of the fermentation chambers.
In some embodiments, the fermentation air supply system comprises a fan, a sterile air inlet pipeline and an air inlet pipe arranged at the bottom of each fermentation chamber, the air inlet pipes are connected through the sterile air inlet pipeline, and an air filter and a flow meter are arranged on the sterile air inlet pipeline; the flowmeter is arranged between the air filter and the fan.
In some embodiments, the control system comprises a controller, a temperature probe, a pH probe, and an oxygen dissolving probe disposed in the fermentation chamber, a water supply valve disposed in the water supply system, a near air inlet valve and an air outlet valve disposed in the heat sterilization system, an air inlet valve and an air outlet valve disposed in the condensation system, and an oxygen supply valve disposed in the fermentation air supply system, wherein the controller is electrically connected to the temperature probe, the pH probe, the oxygen dissolving probe, the water supply valve, the air inlet valve, the air outlet valve, the water inlet valve, the water outlet valve, and the oxygen supply valve.
The invention provides another technical scheme, namely a movable microbial fermentation method for environmental remediation, which is based on the system and comprises the following steps;
s1, controlling a water supply system to feed water into the fermentation chamber by a control system, stirring by a stirring mechanism, cleaning the fermentation chamber, and draining;
s2, the control system controls the heating sterilization system to introduce high-temperature steam through the coil pipe to perform sterilization operation on the fermentation chamber;
s3, controlling a water supply system to feed water into the fermentation chamber by a control system, wherein the water injection amount is 70-80% of the volume of the fermentation chamber;
s4, putting a culture medium into the fermentation chamber from a feeding port of the fermentation box, and controlling a system to start a stirring mechanism to stir;
s5, the control system controls the heating sterilization system to introduce high-temperature steam through the coil pipe to sterilize the fermentation chamber and the culture medium;
s6, the control system controls the condensing system to introduce condensed water through the coil pipe to cool the fermentation chamber;
s7, completing the microorganism inoculation work through the inoculation port; the control system controls the fermentation gas supply system to supply oxygen and starts the stirring mechanism to stir for microbial culture;
s8, the control system controls the fermentation gas supply system to stop supplying oxygen and closes the stirring mechanism, and the microbial liquid is collected through the discharge port, and the step S1 is repeated.
In some embodiments, in step S7, the control system adjusts the stirring speed of the stirring mechanism, controls the oxygen supply amount of the fermentation gas supply system through the value of the dissolved oxygen probe, and monitors the temperature and pH through the temperature probe and the pH probe during the culturing of the microorganism.
The invention has the following beneficial effects: according to the movable microbial fermentation system for environmental remediation, the fermentation box is provided with the unique two-chamber design, so that the fermentation amount of the microbial inoculum can be effectively controlled according to the actual condition of the process, unnecessary waste is avoided under the conditions that the usage amount of the microbial inoculum is not large each time and the preservation condition is not available on site, and the requirement of the current double-carbon target is met. In addition, the manufacturing cost is low, the fermentation process is convenient to operate, the sterilization, cooling and culture integrated operation of the fermentation materials is not subjected to an external procedure, and the risk of bacterial contamination is controlled to be the lowest. Meanwhile, the system is designed to be integrally movable, so that the transportation and the field use are convenient, particularly, the production and transportation cost of the microbial preparation is greatly reduced aiming at environmental microbial remediation projects with limited remote areas and field conditions, the high-activity microbial preparation can be provided according to the requirements of the projects at any time, and the success rate of the projects is ensured.
Drawings
FIG. 1 is a schematic perspective view of a mobile microbial fermentation system for environmental remediation in accordance with example 1 of the present invention;
FIG. 2 is a schematic view showing the internal plan structure of a mobile microbial fermentation system for environmental remediation in example 1 of the present invention;
FIG. 3 is a schematic plan view showing a mobile microbial fermentation system for environmental remediation in example 1 of the present invention.
Detailed Description
In order that the objects, aspects and advantages of the present invention will become more apparent, the present invention will be further described in detail with reference to the following detailed description of preferred embodiments thereof, with reference to the accompanying drawings of fig. 1-3.
Example 1
The movable microbial fermentation system for environmental remediation provided by the embodiment has the following specific technical scheme:
a movable microorganism fermentation system for environmental remediation comprises a fermentation box 1, a water supply system, a heating and sterilizing system, a condensing system, a fermentation gas supply system and a control system 13; the fermentation box 1 is provided with two fermentation chambers, and each fermentation chamber is provided with a coil 6 and a stirring mechanism 14; the water supply system supplies water to the fermentation chamber; the heating sterilization system introduces high-temperature steam through the coil 6 to perform sterilization operation on the fermentation chamber; the condensing system is introduced with condensed water through the coil 6 to cool the fermentation chamber; the fermentation gas supply system is used for supplying oxygen to the fermentation chambers, and the control system 13 controls one or two fermentation chambers to supply water, sterilize, cool and supply oxygen according to the fermentation amount, and controls the start and stop of the stirring mechanism 14.
Specifically, be equipped with baffle 2 in the fermenting case 1, baffle 2 separates fermenting case 1 for two fermentation cavity, and the gas vent is seted up at 1 top of fermenting case, and two fermentation cavity tops communicate each other and all communicate with gas vent 15. The top of the fermentation box 1 is provided with two feed ports 5, and the two feed ports 5 are respectively aligned to the tops of the two fermentation chambers; two material discharge ports 16 are formed in the bottom of the fermentation box 1, the two material discharge ports 16 are respectively aligned with the bottoms of the two fermentation chambers, and the material discharge ports 16 are configured to discharge products from the fermentation chambers or take out samples; two inoculation openings 12 are further formed in the top of the fermentation box 1, and the two inoculation openings 12 are respectively aligned to the tops of the two fermentation chambers. The fermentation box in this embodiment is a stainless steel box body with a heat-insulating layer.
Referring to fig. 1, the movable microbial fermentation system for environmental remediation mainly comprises a fermentation box 1 with a heat-insulating layer, a partition plate 2, a control system 13 and accessory facilities (an overhaul ladder stand 17 and a safety guardrail 18), wherein the fermentation box 1 with the heat-insulating layer is averagely divided into two independent fermentation chambers by the partition plate 2, the two independent fermentation chambers can be independently used according to the fermentation amount, the top of the partition plate 2 is spaced from the top plate of the fermentation box 1 by a certain distance, so that fermentation tail gas can be conveniently and smoothly discharged, and the device is connected into a complete fermentation system through a pipeline system, valves and circuits.
Specifically, the heating sterilization system comprises a steam generator 22, a steam inlet pipeline 7 and a steam outlet pipeline 8, wherein the steam generator 22 is respectively communicated with the top port of each coil 6 through the steam inlet pipeline 7, and the bottom port of each coil 6 is communicated with the steam outlet pipeline 8. The condensing system comprises a condensed water inlet pipeline 9 and a condensed water outlet pipeline 10 which are connected with a tap water source, wherein the condensed water inlet pipeline 9 is respectively communicated with the bottom port of each coil 6, and the condensed water outlet pipeline 10 is respectively communicated with the top port of each coil 6. The water supply system comprises a water inlet pipeline 3 connected with a tap water source, and the water inlet pipeline 3 is communicated with the bottom of each fermentation chamber. The fermentation air supply system comprises a fan 24, a sterile air inlet pipeline 11 and air inlet pipes 4 arranged at the bottom of each fermentation chamber, the air inlet pipes 4 are connected through the sterile air inlet pipeline 11, and an air filter 26 and the sterile air inlet pipeline 11 are arranged on the sterile air inlet pipeline 11; the flow meter 25 is provided between the air filter 26 and the fan 24.
The pipeline connecting mode: the water inlet pipeline 3 is connected with a control system 13, a water pump 23, a water supply valve and a pipeline and is respectively connected into two fermentation chambers from the bottom of the fermentation box 1 with the heat insulation layer to form a water supply system; the steam inlet pipeline 7 is connected with the control system 13, the steam generator 22 and the pipeline, is respectively connected into two fermentation chambers from the top of the fermentation box 1 with the heat-insulating layer, is connected with the coil pipe 6 and then is connected with the steam outlet pipeline 8 at the bottom of the fermentation box 1 to form a heating and sterilizing system; the condensed water inlet pipeline 9 is connected with a control system 13, a water pump 23, a water inlet valve, a water outlet valve and pipelines, is respectively connected into two fermentation chambers from the bottom of the fermentation box 1 with the heat-insulating layer, is connected with the coil pipe 6 and then is connected with a condensed water outlet pipeline 10 at the top of the fermentation box 1 to form a condensation system, and the condensation system and the heating sterilization system share a set of connecting pipelines except for an air inlet, an air outlet and a water inlet; aseptic air inlet pipeline 11 and control system 13, fan 24, flowmeter 25, air cleaner, oxygen supply valve and pipe connection insert two fermentation chambers respectively from taking the heat preservation fermenting case 1 bottom, are connected with intake pipe 4, and intake pipe 4 is foraminiferous stainless steel pipe, constitutes fermentation air supply system.
Specifically, the control system 13 includes a controller, a temperature probe 19, a pH probe 20, a dissolved oxygen probe 21, a water supply valve, an air inlet valve and an air outlet valve, and an oxygen supply valve, wherein the temperature probe 19, the pH probe 20, the dissolved oxygen probe 21, the water supply valve, the air inlet valve, the air outlet valve, the water inlet valve, the water outlet valve, and the oxygen supply valve are disposed in the fermentation chamber.
Before the system is used, the opening and closing of a water supply valve, an air inlet valve, an air outlet valve, a water inlet valve, a water outlet valve and an oxygen supply valve are controlled by a control system 13, so that the whole pipeline system is in a communicated state, a heating and sterilizing system is opened to perform primary sterilization treatment on a fermentation box 1 and all pipeline connecting systems by using steam, and then a steam generator 22 and all valves are closed; tap water is supplied into the fermentation chambers by controlling a water supply system, one or two chambers are selected according to the fermentation amount for water supply operation, and the water injection amount is not more than 70-80% of each fermentation chamber; adding a culture medium from a feeding port 5 according to different types and modes of culturing microorganisms, and starting a stirring mechanism 14 (the stirring mechanism 14 comprises a stirring motor positioned at the top of the fermentation box 1 and a stirring paddle arranged in the fermentation box 1, and the stirring motor drives the stirring paddle to rotate), so that the culture medium is fully mixed and dissolved; starting a heating sterilization system, sterilizing the culture medium, wherein the high-heat sterilization time is not less than 2-4h, and verifying the sterilization effect under the condition (microscopic examination and other modes); after sterilization, starting a condensation system, and reducing the temperature of the fermentation liquor and the system to 25-37 ℃; completing microorganism inoculation work through the inoculation ports 12-1 and 12-2; starting a fermentation gas supply system to carry out microorganism culture work, wherein parameters such as system temperature, pH value, dissolved oxygen and the like are respectively monitored by a temperature probe 19, a pH value probe 20 and a dissolved oxygen probe 21; the fermentation tail gas is discharged out of the system through an exhaust port 15 of the fermentation box 1; the fermentation process is monitored regularly through a sampling port 16; after the fermentation is finished, a microbial fermentation product is obtained through the discharge opening 16.
Example 2
The embodiment provides a movable microorganism fermentation method for environmental remediation based on the system of embodiment 1, and the specific technical scheme is as follows:
the method comprises the steps of utilizing the screened ammonia nitrogen degrading strain for water pollution treatment, carrying out primary liquid amplification culture (NA culture medium), starting the fermentation system, cleaning the system before use through a water inlet system and a stirring mechanism, and controlling a water inlet valve through a control systemOpening and closing to enable the whole pipeline system to be in a communicated state, starting a steam generator, carrying out primary sterilization treatment on the fermentation box 1 and all pipeline connecting systems by using steam through a heating sterilization system, wherein the sterilization time is 30-60min, and then closing a steam source and all water inlet valves; tap water is supplied into the fermentation chambers by controlling a water supply system, and two chambers are selected for water supply operation, wherein the water injection amount is 70-80% of the volume of the chambers; adding NA culture medium from two feeding ports, and starting stirring mechanism to fully mix and dissolve the culture medium for 15-30 min; starting a heating sterilization system, sterilizing the culture medium, wherein the high-heat sterilization time is 3-4h, sampling from a sampling port after sterilization is finished, and performing microscopic examination on the sterilization effect of the system, wherein the sterilization rate is not lower than 98-100%; after sterilization, starting a condensation system, and reducing the temperature of the fermentation liquor and the system to 28-35 ℃; completing microorganism inoculation work through an inoculation port, wherein the inoculation amount is 1 per mill-1%; starting a fermentation gas supply system and a stirring mechanism, controlling the dissolved oxygen to be 40-70%, the rotating speed to be 120-; monitoring the fermentation process through a sampling port every 12-24 h; fermenting for 48-72h, stopping ventilation and stirring after fermentation is finished, collecting microbial liquid through a discharge port, cleaning a fermentation box and sterilizing, and discharging sewage to obtain the ammonia nitrogen degrading microbial inoculum with microbial biomass of 6.3-55 × 108cfu/g。
In conclusion, the movable microbial fermentation system for environmental remediation, provided by the invention, has the advantages that the fermentation box is provided with the unique two-chamber design, the fermentation amount of the microbial inoculum can be effectively controlled according to the actual condition of the process, when the usage amount of the microbial inoculum is not large each time and the field does not have storage conditions, unnecessary waste is avoided, and the requirements of the current double-carbon target are met. In addition, the cost is low, the fermentation process is convenient to operate, the sterilization, cooling and culture integrated operation of the fermentation materials is not performed by an external program, and the contamination risk is controlled to be the lowest. Meanwhile, the system is designed to be integrally movable, so that the transportation and the field use are convenient, particularly, the production and transportation cost of the microbial preparation is greatly reduced aiming at environmental microbial remediation projects with limited remote areas and field conditions, the high-activity microbial preparation can be provided according to the requirements of the projects at any time, and the success rate of the projects is ensured.
The above description is only for the purpose of illustrating preferred embodiments of the present invention and is not to be construed as limiting the invention, and the present invention is not limited to the above examples, and those skilled in the art should also be able to make various changes, modifications, additions or substitutions within the spirit and scope of the present invention.
Claims (10)
1. A movable microbial fermentation system for environmental remediation is characterized by comprising a fermentation box, a water supply system, a heating and sterilizing system, a condensing system, a fermentation gas supply system and a control system; the fermentation box is provided with two fermentation chambers, and each fermentation chamber is provided with a coil pipe and a stirring mechanism; the water supply system supplies water into the fermentation chamber; the heating sterilization system is used for introducing high-temperature steam through the coil pipe to perform sterilization operation on the fermentation chamber; the condensing system is used for introducing condensed water through the coil pipe to cool the fermentation chamber; the fermentation air supply system is used for supplying oxygen to the fermentation chamber, the control system controls one or two fermentation chambers according to the fermentation amount to supply water to operate, the sterilization operation the cooling operation with the oxygen supply operation, and control start and stop of the stirring mechanism.
2. The movable microbial fermentation system for environmental remediation of claim 1, wherein a partition is arranged in the fermentation tank, the partition divides the fermentation tank into two fermentation chambers, an air outlet is formed in the top of the fermentation tank, and the tops of the two fermentation chambers are communicated with each other and are communicated with the air outlet.
3. The movable microbial fermentation system for environmental remediation of claim 1, wherein the top of the fermentation box is provided with two feeding ports, and the two feeding ports are respectively aligned with the tops of the two fermentation chambers; two discharge ports are formed in the bottom of the fermentation box and are respectively aligned with the bottoms of the two fermentation chambers, and the discharge ports are configured to discharge products from the fermentation chambers or take out samples; two inoculation ports are further formed in the top of the fermentation box, and the two inoculation ports are respectively aligned to the tops of the two fermentation chambers.
4. The mobile microbial fermentation system for environmental remediation of claim 1, wherein the heat sterilization system comprises a steam generator, a steam inlet pipeline and a steam outlet pipeline, the steam generator is respectively communicated with the top port of each coil pipe through the steam inlet pipeline, and the bottom port of each coil pipe is communicated with the steam outlet pipeline.
5. The mobile microbial fermentation system for environmental remediation of claim 1, wherein the condensing system comprises a condensate inlet line and a condensate outlet line connected to a source of tap water, the condensate inlet line being in communication with the bottom port of each of the coils, respectively, and the condensate outlet line being in communication with the top port of each of the coils, respectively.
6. The mobile microbial fermentation system for environmental remediation of claim 1, wherein the water supply comprises a water inlet line connected to a source of tap water, the water inlet line communicating with the bottom of each of the fermentation chambers.
7. The movable microbial fermentation system for environmental remediation of claim 1, wherein the fermentation air supply system comprises a fan, a sterile air inlet pipeline and an air inlet pipe arranged at the bottom of each fermentation chamber, the air inlet pipes are connected through the sterile air inlet pipeline, and an air filter and a flow meter are arranged on the sterile air inlet pipeline; the flowmeter is arranged between the air filter and the fan.
8. The movable microbial fermentation system for environmental remediation of claim 1, wherein the control system comprises a controller, a temperature probe, a pH probe and a dissolved oxygen probe disposed in the fermentation chamber, a water supply valve disposed in the water supply system, a near air inlet valve and an air outlet valve disposed in the heating and sterilization system, an air inlet valve and an air outlet valve disposed in the condensation system, and an oxygen supply valve disposed in the fermentation and air supply system, and the controller is electrically connected to the temperature probe, the pH probe, the dissolved oxygen probe, the water supply valve, the air inlet valve, the air outlet valve, the water inlet valve, the water outlet valve, and the oxygen supply valve.
9. A mobile microbial fermentation process for environmental remediation, based on the system of any one of claims 1-8, comprising the steps of;
s1, controlling a water supply system to feed water into the fermentation chamber by a control system, stirring by a stirring mechanism, cleaning the fermentation chamber, and draining;
s2, the control system controls the heating sterilization system to introduce high-temperature steam through the coil pipe to perform sterilization operation on the fermentation chamber;
s3, controlling a water supply system to feed water into the fermentation chamber by a control system, wherein the water injection amount is 70-80% of the volume of the fermentation chamber;
s4, putting a culture medium into the fermentation chamber from a feeding port of the fermentation box, and controlling a system to start a stirring mechanism to stir;
s5, the control system controls the heating sterilization system to introduce high-temperature steam through the coil pipe to sterilize the fermentation chamber and the culture medium;
s6, the control system controls the condensing system to introduce condensed water through the coil pipe to cool the fermentation chamber;
s7, completing the microorganism inoculation work through the inoculation port; the control system controls the fermentation gas supply system to supply oxygen and starts the stirring mechanism to stir for microbial culture;
s8, the control system controls the fermentation gas supply system to stop supplying oxygen and closes the stirring mechanism, and the microbial liquid is collected through the discharge port, and the step S1 is repeated.
10. The mobile microbial fermentation method for environmental remediation, as claimed in claim 9, wherein in step S7, the control system adjusts the stirring speed of the stirring mechanism, controls the oxygen supply amount of the fermentation gas supply system by the value of the dissolved oxygen probe, and monitors the temperature and pH by the temperature probe and the pH probe during the microbial cultivation process.
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| CN202111394465.6A CN114085764A (en) | 2021-11-23 | 2021-11-23 | Movable microbial fermentation system and method for environmental remediation |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204079985U (en) * | 2014-09-22 | 2015-01-07 | 江苏麦可博生物环保工程技术有限公司 | A kind of microbial solid fermentation system |
| CN204768616U (en) * | 2015-06-25 | 2015-11-18 | 宁波大学 | Environment -friendly chemical industry reaction unit with heating cooling device |
| KR20200143933A (en) * | 2019-06-17 | 2020-12-28 | 재단법인 전남바이오산업진흥원 | Microorganism automatic cultivation and distribution system |
| CN213832981U (en) * | 2020-10-21 | 2021-07-30 | 蒙牛高科乳制品（北京）有限责任公司 | Convenient movable microbial fermentation tank |
-
2021
- 2021-11-23 CN CN202111394465.6A patent/CN114085764A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204079985U (en) * | 2014-09-22 | 2015-01-07 | 江苏麦可博生物环保工程技术有限公司 | A kind of microbial solid fermentation system |
| CN204768616U (en) * | 2015-06-25 | 2015-11-18 | 宁波大学 | Environment -friendly chemical industry reaction unit with heating cooling device |
| KR20200143933A (en) * | 2019-06-17 | 2020-12-28 | 재단법인 전남바이오산업진흥원 | Microorganism automatic cultivation and distribution system |
| CN213832981U (en) * | 2020-10-21 | 2021-07-30 | 蒙牛高科乳制品（北京）有限责任公司 | Convenient movable microbial fermentation tank |
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