CN114027099A - Culture medium for reducing fruiting stubbles of agrocybe cylindracea - Google Patents
Culture medium for reducing fruiting stubbles of agrocybe cylindracea Download PDFInfo
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- CN114027099A CN114027099A CN202111417568.XA CN202111417568A CN114027099A CN 114027099 A CN114027099 A CN 114027099A CN 202111417568 A CN202111417568 A CN 202111417568A CN 114027099 A CN114027099 A CN 114027099A
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention provides a culture medium for reducing the fruiting stubbles of agrocybe cylindracea, which comprises main materials and auxiliary materials, wherein the main materials comprise hypsizygus marmoreus mushroom dregs, cottonseed hulls and wood dust, and the auxiliary materials comprise bran, corn flour and gypsum, and the weight percentage of each component is as follows: 15-20% of hypsizygus marmoreus bacterial residues, 29-34% of cottonseed hulls, 20-25% of sawdust, 17-22% of bran, 7% of corn flour and 2% of gypsum, wherein the hypsizygus marmoreus bacterial residues are fresh hypsizygus marmoreus bacterial residues, and are aired until the water content is lower than 20%. When the culture medium is used for cultivating the agrocybe aegerita, the fruiting amount of a single agrocybe aegerita stubble can be increased by 67-93%, the fruiting stubble number of the agrocybe aegerita can be shortened, and the pollution rate of a fungus bag is reduced.
Description
Technical Field
The invention relates to a culture medium for reducing the fruiting stubbles of agrocybe cylindracea, and belongs to the technical field of edible fungus cultivation.
Background
The agrocybe aegerita is an edible fungus with high protein, low fat and low sugar content, and the agrocybe aegerita stem is crisp, tender and tasty and has fragrant taste. The agrocybe aegerita contains the highest content of nutritional ingredients, namely protein, 18 amino acids required by a human body, particularly 8 amino acids which cannot be synthesized by the human body, wherein the highest content of the amino acids is glutamic acid, the content of the total amino acids reaches 17.5%, and the agrocybe aegerita has the effects of cancer prevention, cancer resistance, aging resistance and the like. The fruiting period of the traditional bag cultivation of agrocybe aegerita is 4-6 months, and 5-8 times of agrocybe aegerita can be continuously harvested. Due to the long fruiting period, the pollution rate of fungus bags in the later period of agrocybe cylindracea cultivation (after 3 times of harvest) is high, and diseases and insect pests are easy to occur. Therefore, the key problem of promoting the green high-quality development of the agrocybe cylindracea is how to promote the fruiting yield of the single agrocybe cylindracea, promote the fruiting yield of the single agrocybe cylindracea and reduce the fruiting stubble number of the agrocybe cylindracea.
The edible fungus residue refers to the waste left after various fungi are cultivated, namely the edible fungus waste. According to statistics, in 2019, the yield of the fresh edible mushrooms in China reaches 10.76 ten thousand tons, and the annual yield of the waste mushroom residues is about 20 ten thousand tons. Factory hypsizygus marmoreus production generally produces only one crop in order to consider the yield, so that hypsizygus marmoreus dregs also contain many unused nutrient components. The related research shows that the total carbon content of the mushroom dregs after cultivation is 12.1 percent, the total carbon content is 1.5 percent, and the carbon-nitrogen ratio is 8.07. In addition, the mushroom dregs have the advantages of good air permeability, high water content and the like. Therefore, the hypsizygus marmoreus mushroom residues are rich in nutrition and can provide required nutrition for the growth and development of most edible mushrooms. The deep utilization of the resource has obvious economic and ecological values.
Disclosure of Invention
The invention aims to provide a culture medium for reducing the fruiting number of agrocybe cylindracea, and the culture medium is adopted for cultivating the agrocybe cylindracea, so that the problem that the existing seafood mushroom residues pollute the environment is solved, the production cost of the agrocybe cylindracea is reduced, the single-stubble yield of the agrocybe cylindracea is effectively improved, the harvesting times are reduced, the fruiting time is shortened, and the pollution rate of fungus bags is reduced.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a culture medium for reducing the fruiting stubbles of agrocybe cylindracea, which comprises main materials and auxiliary materials, wherein the main materials comprise hypsizygus marmoreus mushroom dregs, cottonseed hulls and sawdust, and the auxiliary materials comprise bran, corn flour and gypsum, and the weight percentage of each component is as follows: 15-20% of hypsizygus marmoreus bacterial residues, 29-34% of cottonseed hulls, 20-25% of sawdust, 17-22% of bran, 7% of corn flour and 2% of gypsum, wherein the hypsizygus marmoreus bacterial residues are fresh hypsizygus marmoreus bacterial residues, and are aired until the water content is lower than 20%.
The invention also provides a preparation method of the culture medium for reducing the fruiting number of the agrocybe cylindracea, which comprises the following steps:
1) pretreatment of mushroom dregs: crushing the hypsizygus marmoreus residues for later use;
2) burdening and mixing: mixing pretreated hypsizygus marmoreus mushroom dregs, cottonseed hulls and sawdust in proportion, adding water for prewetting, adding bran, corn flour and gypsum in proportion, stirring uniformly, and adding water to adjust the water content and pH of the mixed material to obtain a wet material;
3) bagging: pouring the wet material into a polypropylene plastic bag by adopting a manual or mechanical mode to obtain a material bag;
4) and (3) sterilization: sterilizing the material bag by adopting a high-pressure steam sterilization method;
5) and (3) cooling: and cooling the sterilized material bags to obtain the culture medium for reducing the fruiting stubbles of the agrocybe cylindracea.
Further, in the step 1) of the preparation method, the hypsizygus marmoreus mushroom dregs are crushed to the granularity of 2-6 mm.
Further, in the step 2) of the preparation method, the main material is pre-wetted for 2-3 hours before the main material and the auxiliary materials are mixed, so that the water content of the main material reaches 60% -66%.
Further, in the step 2) of the preparation method, the moisture content of the wet material is 62-65, and the pH value is 7.5-8.0.
Further, in step 3) of the above preparation method, the polyethylene plastic bag with the specification of 17.5cm × 55cm × 0.05mm is used for filling, and the polyethylene plastic bag is packed according to 1050-.
Further, in step 4) of the above preparation method, the sterilization condition is 128 ℃ for 200 min.
Further, in step 5) of the above preparation method, the sterilized material bag is cooled to 30 ℃ or lower.
The invention also provides application of the culture medium for reducing the fruiting number of the agrocybe cylindracea in agrocybe cylindracea production.
The invention has the following remarkable advantages:
(1) the hypsizygus marmoreus dregs contain a large amount of substances such as xylanase, carboxymethyl cellulose and the like, and can be subjected to pre-degradation on sawdust and cottonseed hulls through pre-wet fermentation for 2-3 hours by virtue of a pre-mixed material, so that micromolecular substances such as reducing sugar and the like are generated, and meanwhile, the fungus dregs contain the reducing sugar, so that the substances are beneficial to utilization of agrocybe cylindracea hyphae, rapid fungus growing and field planting of the agrocybe cylindracea can be realized, and the pollution rate of fungus bags is reduced.
(2) The hypsizygus marmoreus dregs contain a large amount of crude fibers which are beneficial to growth of the agrocybe aegerita, and can provide a high-quality nitrogen source for growth of the agrocybe aegerita.
(3) The hypsizygus marmoreus mushroom dregs have certain granularity, so that the air permeability of the mushroom bag can be increased, the activity of hyphae is enhanced, and the consistency of mushroom cultivation is ensured.
(4) The invention can solve the pollution problem of the hypsizygus marmoreus mushroom dregs and reduce the production cost of the agrocybe aegerita.
In conclusion, through reasonable matching of the raw materials among the formulas, the growth speed and the nutrient accumulation of hyphae are improved while the nutrient components required by growth of agrocybe aegerita are provided, the consistency and the stability of fungus walking are ensured, good nutrient guarantee is provided for fruiting of agrocybe aegerita, the fruiting yield of a single stubble is improved, the number of fruiting stubbles is shortened, the running efficiency of a storehouse is improved, the problem of fungus bag pollution easily caused in the later period of fruiting of agrocybe aegerita is reduced, pesticide use is reduced, and green, safe and efficient development of agrocybe aegerita is promoted.
Drawings
FIG. 1: the growth of the mycelial growth of agrocybe aegerita in example 2.
FIG. 2: the soluble protein content of the media of different formulations varied in example 3.
FIG. 3: the pH of the media of the different formulations of example 3 varied.
FIG. 4: the first crop of agrocybe aegerita in example 3 showed fruiting.
Detailed Description
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
Example 1
After the fruiting bodies of the hypsizygus marmoreus are mature, sampling is continuously carried out for 3 days to obtain fresh mildew-free hypsizygus marmoreus residues of 3 different production batches, and the fresh mildew-free hypsizygus marmoreus residues are crushed by a crusher for later use. Adding 20.00 g of crushed fresh hypsizygus marmoreus residues into RO water according to a material-liquid ratio of 1:5 (m/v), extracting for 2 h at 25 ℃ of a shaking table and under vibration at 150 rpm/min, filtering the extract liquid through two layers of gauze to remove the material residues, centrifuging the filtrate at 4000 rpm/min for 15 min, obtaining a supernatant, namely a hypsizygus marmoreus residue extract, and measuring the pH value, the lignin peroxidase activity and the cellulase activity of the extract. Taking another 5.00 g of crushed fresh hypsizygus marmoreus residues, and measuring the water content and the crude fiber content of the fresh hypsizygus marmoreus residues by adopting a conventional method. Meanwhile, the pleurotus geesteranus mushroom dregs and the pleurotus eryngii mushroom dregs are measured by the same method. The results are shown in Table 1.
TABLE 1 determination of the composition of the fungus dregs of different edible fungi
As can be seen from Table 1, the content of crude fiber in the hypsizygus marmoreus dregs is high, and simultaneously, the activity of lignin peroxidase and the activity of cellulase for degrading lignin are both obviously higher than those of pleurotus geesteranus dregs and pleurotus eryngii dregs. Therefore, the hypsizygus marmoreus mushroom dregs are selected to prepare the agrocybe aegerita culture medium, so that the culture medium can provide nutrition for the growth of the agrocybe aegerita and can be used for pre-degrading the wood chips and the cottonseed hulls in the main material of the agrocybe aegerita culture medium.
Example 2 Effect of different formulations of Medium on Agrocybe aegerita hypha growth
The embodiment provides a hypsizygus marmoreus mushroom dreg culture medium for reducing the fruiting stubble number of agrocybe cylindracea, which comprises main materials and auxiliary materials, wherein the main materials are hypsizygus marmoreus mushroom dregs, cottonseed hulls and wood chips, the auxiliary materials are bran, corn flour and gypsum, and the culture medium comprises the following components in percentage by weight: 15% of hypsizygus marmoreus bacterial residues, 34% of cottonseed hulls, 20% of sawdust, 22% of bran, 7% of corn flour and 2% of gypsum, wherein the hypsizygus marmoreus bacterial residues are fresh and mildew-free hypsizygus marmoreus bacterial residues, and are aired until the water content is lower than 20%. The preparation method of the culture medium comprises the following steps:
1) pretreatment of mushroom dregs: pulverizing the hypsizygus marmoreus residues to granularity of 2-6mm for later use;
2) burdening and mixing: mixing pretreated hypsizygus marmoreus mushroom dregs, cottonseed hulls and sawdust in proportion, adding water for prewetting for 2-3h to enable the water content of the main material to reach 60% -66%, adding bran, corn flour and gypsum in proportion, stirring uniformly, adding water to adjust the water content and the pH value of the mixed material to obtain a wet material, wherein the water content of the wet material is 62% -65%, and the pH value is 7.5-8.0;
3) bagging: manually or mechanically pouring the wet material into a glass test tube with the specification of 40mm multiplied by 200mm according to 30 g/tube to obtain split charging wet material;
4) and (3) sterilization: sterilizing the packaged wet materials in a high temperature and high pressure sterilizing pot at 128 deg.C for 200 min;
5) and (3) cooling: and cooling the sterilized wet material to be below 28 ℃ to obtain the culture medium for reducing the fruiting stubbles of the agrocybe cylindracea.
To examine the advantages of the culture medium for reducing the number of fruiting stubbles of agrocybe aegerita described in this example, two control medium tests were performed simultaneously.
The contrast group culture medium 1 is a pleurotus eryngii mushroom dreg culture medium, the main materials of the contrast group culture medium are pleurotus eryngii mushroom dreg, cottonseed hulls and wood dust, and the auxiliary materials are bran, corn flour and gypsum, wherein the weight percentage of each component is as follows: the pleurotus eryngii mushroom dreg curing agent comprises 15% of pleurotus eryngii mushroom dreg, 34% of cottonseed hulls, 20% of sawdust, 22% of bran, 7% of corn flour and 2% of gypsum, wherein the pleurotus eryngii mushroom dreg is a material which is prepared by airing fresh and mildew-free pleurotus eryngii mushroom dreg and has the water content of lower than 20%. The preparation method of the culture medium comprises the following steps:
1) pretreatment of mushroom dregs: crushing pleurotus eryngii fungus residues to the granularity of 2-6mm for later use;
2) burdening and mixing: mixing pretreated pleurotus eryngii dregs, cottonseed hulls and sawdust in proportion, adding water for prewetting for 2-3 hours to enable the water content of the main material to reach 60% -66%, adding bran, corn flour and gypsum in proportion, stirring uniformly, adding water to adjust the water content and the pH value of the mixed material to obtain a wet material, wherein the water content of the wet material is 62% -65%, and the pH value is 7.5-8.0;
3) bagging: manually or mechanically pouring the wet material into a glass test tube with the specification of 40mm multiplied by 200mm according to 30 g/tube to obtain split charging wet material;
4) and (3) sterilization: sterilizing the packaged wet materials in a high temperature and high pressure sterilizing pot at 128 deg.C for 200 min;
5) and (3) cooling: and cooling the sterilized wet material to be below 28 ℃ to obtain the pleurotus eryngii fungi residue culture medium.
The control group culture medium 2 is a traditional culture medium, the main materials of the culture medium are cottonseed hulls, sawdust and wheat bran, the auxiliary materials are corn flour and gypsum, and the weight percentage of each component is 40% of the cottonseed hulls, 30% of the sawdust, 20% of the wheat bran, 8% of the corn flour and 2% of the gypsum. The preparation method of the culture medium comprises the following steps:
1) burdening and mixing: mixing cottonseed hull, sawdust and wheat bran in proportion, adding water for pre-wetting for 2-3h to make the water content of the main material reach 60% -66%, adding corn flour and gypsum in proportion, stirring uniformly, adding water to adjust the water content and pH of the mixed material to obtain a wet material, wherein the water content of the wet material is 62% -65%, and the pH is 7.5-8.0;
2) bagging: manually or mechanically pouring the wet material into a glass test tube with the specification of 40mm multiplied by 200mm according to 30 g/tube to obtain split charging wet material;
3) and (3) sterilization: sterilizing the packaged wet materials in a high temperature and high pressure sterilizing pot at 128 deg.C for 200 min;
4) and (3) cooling: and cooling the sterilized wet material to be below 28 ℃ to obtain the traditional cultivation medium.
Respectively inoculating the activated solid strain of the agrocybe cylindracea into a hypsizygus marmoreus dreg culture medium, an pleurotus eryngii dreg culture medium and a traditional cultivation culture medium, and inoculating 1 strain block of 0.5cm multiplied by 0.5cm into each test tube. Subsequently, the test tubes were grouped and placed in an incubator at 26 ℃ in the absence of light for cultivation. The growth of the mycelia of Agrocybe aegerita in different media is shown in FIG. 1 and Table 2.
TABLE 2 mycelial growth of Agrocybe aegerita in different media
As can be seen from fig. 1 and table 2, the growth rate of the hyphae of the agrocybe aegerita in the hypsizygus marmoreus residue culture medium is higher than that of the traditional culture medium, and the growth vigor, the color and the robustness of the hyphae are better than those of the traditional culture medium; the growth rate of hypha of the agrocybe cylindracea in the flammulina velutipes fungus residue culture medium is lower than that of the traditional culture medium. The results show that different culture media have different influences on the growth of the agrocybe aegerita hyphae, the edible fungi not only utilize carbon sources and nitrogen sources in the matrix in the growth process, but also can generate certain secondary metabolites, the secondary metabolites generated in the growth process of the agrocybe aegerita can inhibit the growth of the agrocybe aegerita hyphae to a certain degree, and the hypsizygus marmoreus dregs can provide nutrition for the agrocybe aegerita hyphae, can not inhibit the growth of the agrocybe aegerita hyphae, and can be used as a good matrix raw material for agrocybe aegerita cultivation.
Example 3 Effect of different formulations of Medium on Agrocybe aegerita yield and agronomic traits
The embodiment provides a hypsizygus marmoreus mushroom dreg culture medium for reducing the fruiting stubble number of agrocybe cylindracea, which comprises main materials and auxiliary materials, wherein the main materials are hypsizygus marmoreus mushroom dregs, cottonseed hulls and wood chips, the auxiliary materials are bran, corn flour and gypsum, and the culture medium comprises the following components in percentage by weight: the production method comprises the following steps of (1) drying fresh hypsizygus marmoreus mushroom dregs, wherein the fresh hypsizygus marmoreus mushroom dregs comprise 15% of the hypsizygus marmoreus mushroom dregs, 34% of cottonseed hulls, 20% of sawdust, 22% of bran, 7% of corn flour and 2% of gypsum, and the fresh hypsizygus marmoreus mushroom dregs are materials with the moisture content being lower than 20% after being dried in the sun. The preparation method of the culture medium comprises the following steps:
1) pretreatment of mushroom dregs: pulverizing the hypsizygus marmoreus residues to granularity of 2-6mm for later use;
2) burdening and mixing: mixing pretreated hypsizygus marmoreus mushroom dregs, cottonseed hulls and sawdust in proportion, adding water for prewetting for 2-3h to enable the water content of the main material to reach 60% -66%, adding bran, corn flour and gypsum in proportion, stirring uniformly, adding water to adjust the water content and the pH value of the mixed material to obtain a wet material, wherein the water content of the wet material is 62% -65%, and the pH value is 7.5-8.0;
3) bagging: pouring the wet material into a polypropylene plastic bag with the specification of 17.0cm multiplied by 33cm multiplied by 0.05mm by adopting a manual or mechanical mode according to 1050 plus 1150 g/bag, and packaging the wet material to be 17.0-18.0cm in height to obtain a material bag;
4) and (3) sterilization: sterilizing the material bag in a high temperature and high pressure sterilizing pot at 128 deg.C for 200 min;
5) and (3) cooling: and cooling the sterilized material bags to be below 30 ℃ to obtain the culture medium for reducing the fruiting stubbles of the agrocybe cylindracea.
To examine the advantages of the culture medium for reducing the number of fruiting stubbles of agrocybe aegerita described in this example, a set of control medium experiments were performed at the same time.
The control group culture medium is a traditional culture medium and comprises main materials and auxiliary materials, wherein the main materials are cottonseed hulls, sawdust and wheat bran, the auxiliary materials are corn flour and gypsum, and the weight percentage of each component is as follows: 40% of cottonseed hulls, 30% of sawdust, 20% of wheat bran, 9% of corn flour and 2% of gypsum, and the preparation method of the culture medium comprises the following steps:
1) burdening and mixing: mixing cottonseed hulls, sawdust and wheat bran in proportion, adding water for pre-wetting for 2-3h to make the water content of the main material reach 60% -66%, adding corn flour and gypsum in proportion, stirring uniformly, adding water to adjust the water content and pH of the mixed material to obtain a wet material, wherein the water content of the wet material is 62% -65%, and the pH is 7.5-8.0;
2) bagging: pouring the wet material into a polypropylene plastic bag with the specification of 17.0cm multiplied by 33cm multiplied by 0.05mm by adopting a manual or mechanical mode according to 1050 plus 1150 g/bag, and packaging the wet material to be 17.0-18.0cm in height to obtain a material bag;
3) and (3) sterilization: sterilizing the material bag in a high temperature and high pressure sterilizing pot at 128 deg.C for 200 min;
4) and (3) cooling: and cooling the sterilized material bags to be below 30 ℃ to obtain the traditional cultivation medium.
Punching the activated agrocybe cylindracea solid agar strain by using a puncher with the diameter of 1.2cm, and respectively inoculating the strain into a hypsizygus marmoreus residue culture medium and a traditional cultivation culture medium, wherein 6 pieces of strain are inoculated in each bag. And then, placing the culture mediums in groups in a culture room, setting the temperature to be 24-26 ℃, culturing in a dark place, and performing bag opening and fruiting management according to a conventional method after culturing for 35 d. And harvesting when the fruiting bodies of the mushrooms grow to be mature. During collection, the seeds are picked up by gentle rotation and weighed and recorded. And after the first crop is harvested, performing second crop management and fruiting after the hyphae are recovered for 7d, and weighing and recording.
The soluble protein and pH change during the culture of Agrocybe aegerita with different formula are shown in FIG. 2 and FIG. 3. The first fruiting situation of agrocybe cylindracea is shown in fig. 4, the yield and the agronomic characters are shown in tables 3 and 4, the agrocybe cylindracea is harvested after 2 times under the culture of the hypsizygus marmoreus residue culture medium, and the agrocybe cylindracea is harvested for 5 times under the traditional culture medium.
TABLE 3 Effect of different formulations of media on Agrocybe aegerita yield
TABLE 4 influence of different formula media on Agrocybe aegerita agronomic traits
As can be seen from fig. 2 and 3, during the culture of agrocybe aegerita, the content of soluble protein in the hypsizygus marmoreus dreg culture medium is higher than that of the traditional culture medium, and the content of soluble protein can show the content of extracellular enzyme to a certain extent, which indicates that the hypsizygus marmoreus dreg culture medium for reducing the number of fruiting stubbles of agrocybe aegerita can promote the hypha of the agrocybe aegerita to secrete extracellular enzyme and promote the absorption and utilization of a matrix; meanwhile, the pH value of the hypsizygus marmoreus dreg culture medium is lower, which shows that the utilization efficiency of the agrocybe cylindracea to the matrix is high, and the result is consistent with the change of soluble protein.
As can be seen from FIG. 4, the agrocybe cylindracea fruiting yield and uniformity of the hypsizygus marmoreus dreg culture medium are obviously superior to those of the traditional culture medium in the same fruiting time. As can be seen from tables 3 and 4, the yields of the agrocybe cylindracea are obviously different under the culture of culture media with different formulas, and under the culture of the hypsizygus marmoreus dreg culture medium, the yield of the agrocybe cylindracea in the first crop can reach 249.60g, is improved by 67.7% compared with the traditional culture medium, the yield of the agrocybe cylindracea in the second crop is 273.5g, is improved by 97.2% compared with the traditional culture medium, and the pollution rate of a fungus bag after two crops is 2.8%, which is obviously lower than 9.3% of the traditional culture medium; and the agronomic characters such as the diameter of a cap and the diameter of a stem of the collected tea tree mushroom are close to those of the traditional culture medium, so that the yield of a single crop of tea tree mushroom can be effectively improved by adding the hypsizygus marmoreus mushroom dregs, the total yield of fruiting of two crops is 523.1g, which is obviously higher than that of the total yield of five crops of the traditional formula of 496.77g, and the fruiting times can be effectively reduced and the fruiting uniformity can be improved after adding the hypsizygus marmoreus mushroom dregs.
Table 5 shows the effect of different formulation cultures on the yield of agrocybe aegerita. The situation that 2 stubbles are harvested under the culture of the hypsizygus marmoreus residue culture medium and 5 stubbles are harvested under the culture of the traditional culture medium are compared, the culture medium is calculated according to 8 yuan/kg of fresh agrocybe cylindracea products, the harvest times are shortened by utilizing the hypsizygus marmoreus residue culture medium, the single bag cost can be saved by 0.7 yuan, and the single bag profit can be improved by 0.91 yuan.
TABLE 5 Effect of different formulations of media on Agrocybe aegerita yield
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (9)
1. A culture medium for reducing the fruiting number of agrocybe cylindracea is characterized in that: the seafood delicacy feed comprises main materials and auxiliary materials, wherein the main materials comprise seafood delicacy mushroom dregs, cottonseed hulls and sawdust, the auxiliary materials comprise bran, corn flour and gypsum, and the weight percentage of each component is as follows: 15-20% of hypsizygus marmoreus bacterial residues, 29-34% of cottonseed hulls, 20-25% of sawdust, 17-22% of bran, 7% of corn flour and 2% of gypsum, wherein the hypsizygus marmoreus bacterial residues are fresh hypsizygus marmoreus bacterial residues, and are aired until the water content is lower than 20%.
2. A method for preparing a culture medium for reducing the number of fruiting stubbles of agrocybe aegerita according to claim 1, comprising: the method comprises the following steps:
1) pretreatment of mushroom dregs: crushing the hypsizygus marmoreus residues for later use;
2) burdening and mixing: mixing pretreated hypsizygus marmoreus mushroom dregs, cottonseed hulls and sawdust in proportion, adding water for prewetting, adding bran, corn flour and gypsum in proportion, stirring uniformly, and adding water to adjust the water content and pH of the mixed material to obtain a wet material;
3) bagging: pouring the wet material into a polypropylene plastic bag by adopting a manual or mechanical mode to obtain a material bag;
4) and (3) sterilization: sterilizing the material bag by adopting a high-pressure steam sterilization method;
5) and (3) cooling: and cooling the sterilized material bags to obtain the culture medium for reducing the fruiting stubbles of the agrocybe cylindracea.
3. The method of claim 2, wherein: in the step 1), the hypsizygus marmoreus mushroom dregs are crushed to have the granularity of 2-6 mm.
4. The method of claim 2, wherein: in the step 2), the main material is pre-wetted for 2-3 hours before being mixed with the auxiliary material, so that the water content of the main material reaches 60% -66%.
5. The method of claim 2, wherein: in the step 2), the moisture content of the wet material is 62-65%, and the pH value is 7.5-8.0.
6. The method of claim 2, wherein: in the step 3), the polyethylene plastic bags with the specification of 17.5cm multiplied by 55cm multiplied by 0.05mm are used for filling, and the materials are packed according to 1050 plus 1150 g/bag, and the packing height is 17.0-18.0 cm.
7. The method of claim 2, wherein: in step 4), the sterilization condition is 128 ℃ for 200 min.
8. The method of claim 2, wherein: in step 5), cooling the sterilized material bag to below 30 ℃.
9. The use of the culture medium for reducing the number of fruiting stubbles of agrocybe aegerita as claimed in claim 1 in agrocybe aegerita production.
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