CN114008205A - 前导序列 - Google Patents

前导序列 Download PDF

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Publication number
CN114008205A
CN114008205A CN202080042046.3A CN202080042046A CN114008205A CN 114008205 A CN114008205 A CN 114008205A CN 202080042046 A CN202080042046 A CN 202080042046A CN 114008205 A CN114008205 A CN 114008205A
Authority
CN
China
Prior art keywords
pvc
effector
seq
payload
leader sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202080042046.3A
Other languages
English (en)
Chinese (zh)
Inventor
尼古拉斯·R·沃特菲尔德
约瑟·夫希利
亚莉克西亚·哈皮什
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosilinx Ltd
Original Assignee
University of Warwick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Warwick filed Critical University of Warwick
Publication of CN114008205A publication Critical patent/CN114008205A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nanotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medical Informatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
CN202080042046.3A 2019-06-07 2020-06-05 前导序列 Pending CN114008205A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1908155.3A GB201908155D0 (en) 2019-06-07 2019-06-07 Leader sequence
GB1908155.3 2019-06-07
PCT/GB2020/051380 WO2020245611A1 (en) 2019-06-07 2020-06-05 Leader sequence

Publications (1)

Publication Number Publication Date
CN114008205A true CN114008205A (zh) 2022-02-01

Family

ID=67386182

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080042046.3A Pending CN114008205A (zh) 2019-06-07 2020-06-05 前导序列

Country Status (12)

Country Link
US (1) US20230076614A1 (enExample)
EP (1) EP3980545A1 (enExample)
JP (2) JP7748288B2 (enExample)
KR (1) KR20220133757A (enExample)
CN (1) CN114008205A (enExample)
AU (1) AU2020288380A1 (enExample)
BR (1) BR112021024592A2 (enExample)
CA (1) CA3142462A1 (enExample)
GB (1) GB201908155D0 (enExample)
MX (1) MX2021015121A (enExample)
SG (1) SG11202113297RA (enExample)
WO (1) WO2020245611A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025061134A1 (zh) * 2023-09-22 2025-03-27 北京朝赛蕴生物科技有限公司 一种蛋白复合物的制备纯化及应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4479082A2 (en) * 2022-02-15 2024-12-25 The Broad Institute, Inc. Cell-type specific targeting contractile injection system
EP4547848A1 (en) 2022-06-30 2025-05-07 Københavns Universitet Contractile injection system and use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160046951A1 (en) * 2013-03-07 2016-02-18 The General Hospital Corporation Compositions and methods for bacterial delivery of polypeptides
CN108137649A (zh) * 2015-10-14 2018-06-08 拜耳作物科学有限合伙公司 AXMI554δ-内毒素基因及其使用方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160046951A1 (en) * 2013-03-07 2016-02-18 The General Hospital Corporation Compositions and methods for bacterial delivery of polypeptides
CN108137649A (zh) * 2015-10-14 2018-06-08 拜耳作物科学有限合伙公司 AXMI554δ-内毒素基因及其使用方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AKIO ABE 等: ""Type-Ⅲ effectors：Sophisticated bacterial virulence factors"", 《COMPTES RENDUS BIOLOGIES》, vol. 328, pages 414 *
FENG JIANG 等: ""N-terminal signal peptides facilitate the engineering of PVC complex as a potent protein delivery system"", 《SCIENCE ADVANCES》, vol. 8, pages 1 - 11 *
ISABELLA VLISIDOU 等: ""Photorhabdus Virulence Cassettes: extracellular multi-protein needle complexes for delivery of small protein effectors into host cells"", 《BIORXIV》, pages 29 - 31 *
訾祯祯 等: ""细菌蛋白分泌途径的研究进展"", 《生物技术通报》, no. 8, pages 44 - 51 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025061134A1 (zh) * 2023-09-22 2025-03-27 北京朝赛蕴生物科技有限公司 一种蛋白复合物的制备纯化及应用

Also Published As

Publication number Publication date
US20230076614A1 (en) 2023-03-09
BR112021024592A2 (pt) 2022-01-18
JP2022535283A (ja) 2022-08-05
KR20220133757A (ko) 2022-10-05
CA3142462A1 (en) 2020-12-10
WO2020245611A1 (en) 2020-12-10
EP3980545A1 (en) 2022-04-13
AU2020288380A1 (en) 2022-01-06
SG11202113297RA (en) 2021-12-30
JP7748288B2 (ja) 2025-10-02
GB201908155D0 (en) 2019-07-24
JP2025114573A (ja) 2025-08-05
MX2021015121A (es) 2022-03-17

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Applicant before: THE University OF WARWICK