JP7748288B2 - リーダー配列 - Google Patents

リーダー配列

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Publication number
JP7748288B2
JP7748288B2 JP2021572387A JP2021572387A JP7748288B2 JP 7748288 B2 JP7748288 B2 JP 7748288B2 JP 2021572387 A JP2021572387 A JP 2021572387A JP 2021572387 A JP2021572387 A JP 2021572387A JP 7748288 B2 JP7748288 B2 JP 7748288B2
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JP
Japan
Prior art keywords
pvc
seq
effector
payload
needle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021572387A
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English (en)
Japanese (ja)
Other versions
JP2022535283A (ja
Inventor
アール． ウォーターフィールド，ニコラス
ヒーリー，ジョセフ
ハペシー，アレクシア
Original Assignee
ナノシュリンクス リミテッド
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Publication date
Application filed by ナノシュリンクス リミテッド filed Critical ナノシュリンクス リミテッド
Publication of JP2022535283A publication Critical patent/JP2022535283A/ja
Priority to JP2025064761A priority Critical patent/JP2025114573A/ja
Application granted granted Critical
Publication of JP7748288B2 publication Critical patent/JP7748288B2/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nanotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medical Informatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
JP2021572387A 2019-06-07 2020-06-05 リーダー配列 Active JP7748288B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025064761A JP2025114573A (ja) 2019-06-07 2025-04-10 リーダー配列

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1908155.3 2019-06-07
GBGB1908155.3A GB201908155D0 (en) 2019-06-07 2019-06-07 Leader sequence
PCT/GB2020/051380 WO2020245611A1 (en) 2019-06-07 2020-06-05 Leader sequence

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025064761A Division JP2025114573A (ja) 2019-06-07 2025-04-10 リーダー配列

Publications (2)

Publication Number Publication Date
JP2022535283A JP2022535283A (ja) 2022-08-05
JP7748288B2 true JP7748288B2 (ja) 2025-10-02

Family

ID=67386182

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021572387A Active JP7748288B2 (ja) 2019-06-07 2020-06-05 リーダー配列
JP2025064761A Pending JP2025114573A (ja) 2019-06-07 2025-04-10 リーダー配列

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025064761A Pending JP2025114573A (ja) 2019-06-07 2025-04-10 リーダー配列

Country Status (12)

Country Link
US (1) US20230076614A1 (enExample)
EP (1) EP3980545A1 (enExample)
JP (2) JP7748288B2 (enExample)
KR (1) KR20220133757A (enExample)
CN (1) CN114008205A (enExample)
AU (1) AU2020288380A1 (enExample)
BR (1) BR112021024592A2 (enExample)
CA (1) CA3142462A1 (enExample)
GB (1) GB201908155D0 (enExample)
MX (1) MX2021015121A (enExample)
SG (1) SG11202113297RA (enExample)
WO (1) WO2020245611A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023158486A2 (en) * 2022-02-15 2023-08-24 The Broad Institute, Inc. Cell-type specific targeting contractile injection system
CA3260213A1 (en) 2022-06-30 2024-01-04 Københavns Universitet CONTRACTILE INJECTION SYSTEM AND ITS USE
CN119735656A (zh) * 2023-09-22 2025-04-01 北京朝赛蕴生物科技有限公司 一种蛋白复合物的制备纯化及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
AU2014225717B2 (en) * 2013-03-07 2019-01-17 President And Fellows Of Harvard College Compositions and methods for bacterial delivery of polypeptides
CA3001123C (en) * 2015-10-14 2024-01-23 Bayer Cropscience Lp Axmi554 delta-endotoxin gene and methods for its use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DUCHAUD. E. et al.，Nature Biotechnology，2003年，Vol.21, No.11，pp.1307-1313
JUBELIN, G. et al.，PLOS ONE，2009年，Vol.4, No.3, e4855，pp.1-13
VISIDOU, I. et al.，Photorhabdus Virulence Cassettes: extracellular multi-protein needle complexes for delivery of small protein effectors into host cells，bioRxiv preprint，2019年02月15日，doi.org/10.1101/549964

Also Published As

Publication number Publication date
AU2020288380A1 (en) 2022-01-06
BR112021024592A2 (pt) 2022-01-18
SG11202113297RA (en) 2021-12-30
WO2020245611A1 (en) 2020-12-10
MX2021015121A (es) 2022-03-17
US20230076614A1 (en) 2023-03-09
GB201908155D0 (en) 2019-07-24
JP2022535283A (ja) 2022-08-05
CN114008205A (zh) 2022-02-01
KR20220133757A (ko) 2022-10-05
EP3980545A1 (en) 2022-04-13
JP2025114573A (ja) 2025-08-05
CA3142462A1 (en) 2020-12-10

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