CN113994960A - Pharmaceutical composition and pharmaceutical preparation for preventing and treating peanut root-knot nematode and preparation method thereof - Google Patents
Pharmaceutical composition and pharmaceutical preparation for preventing and treating peanut root-knot nematode and preparation method thereof Download PDFInfo
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- 241000243784 Meloidogyne arenaria Species 0.000 title claims abstract description 37
- 239000000825 pharmaceutical preparation Substances 0.000 title claims abstract description 23
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title abstract description 16
- 241000218628 Ginkgo Species 0.000 claims abstract description 40
- 235000011201 Ginkgo Nutrition 0.000 claims abstract description 40
- 235000008100 Ginkgo biloba Nutrition 0.000 claims abstract description 40
- 235000014571 nuts Nutrition 0.000 claims abstract description 30
- 239000000284 extract Substances 0.000 claims abstract description 26
- 239000005959 Fosthiazate Substances 0.000 claims abstract description 20
- DUFVKSUJRWYZQP-UHFFFAOYSA-N fosthiazate Chemical compound CCC(C)SP(=O)(OCC)N1CCSC1=O DUFVKSUJRWYZQP-UHFFFAOYSA-N 0.000 claims abstract description 20
- OICGFSUWXCJLCO-UHFFFAOYSA-N 1-ethylsulfonylsulfanylethane Chemical compound CCSS(=O)(=O)CC OICGFSUWXCJLCO-UHFFFAOYSA-N 0.000 claims abstract description 19
- BWPGKXYWPBQBPV-MWQJAWBESA-N Theasaponin Chemical compound O([C@H]1[C@H](O)[C@H](O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@@H](O)CO1)O[C@H]1[C@@H]([C@@H](O)[C@H](O)CO1)O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2[C@@]1(CO)C)C)(C)C[C@@H](O)[C@@]1(CO)[C@@H](OC(C)=O)[C@@H](C(C[C@H]14)(C)C)OC(=O)C(\C)=C/C)C(O)=O)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BWPGKXYWPBQBPV-MWQJAWBESA-N 0.000 claims abstract description 17
- BWPGKXYWPBQBPV-ZOADXXHESA-N Theasaponin Natural products O=C(O[C@@H]1[C@@H](OC(=O)C)[C@]2(CO)[C@@H](O)C[C@@]3(C)[C@@]4(C)[C@@H]([C@]5(C)[C@H]([C@@](CO)(C)[C@@H](O[C@@H]6[C@@H](O[C@@H]7[C@H](O[C@@H]8[C@@H](O)[C@H](O)[C@H](O)CO8)[C@H](O)[C@@H](O)CO7)[C@@H](O[C@H]7[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O7)[C@H](O)[C@@H](C(=O)O)O6)CC5)CC4)CC=C3[C@@H]2CC1(C)C)/C(=C/C)/C BWPGKXYWPBQBPV-ZOADXXHESA-N 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 11
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000000605 extraction Methods 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 12
- 210000000582 semen Anatomy 0.000 claims description 10
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 239000009429 Ginkgo biloba extract Substances 0.000 claims description 7
- 229940068052 ginkgo biloba extract Drugs 0.000 claims description 7
- 235000020686 ginkgo biloba extract Nutrition 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000003755 preservative agent Substances 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- PLUHAVSIMCXBEX-UHFFFAOYSA-N azane;dodecyl benzenesulfonate Chemical group N.CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 PLUHAVSIMCXBEX-UHFFFAOYSA-N 0.000 claims description 4
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 4
- 238000010992 reflux Methods 0.000 claims description 4
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 claims description 4
- 241001143352 Meloidogyne Species 0.000 claims 1
- 241000244206 Nematoda Species 0.000 abstract description 12
- 230000000607 poisoning effect Effects 0.000 abstract description 5
- 238000013329 compounding Methods 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 10
- 244000105624 Arachis hypogaea Species 0.000 description 8
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 235000020232 peanut Nutrition 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 5
- 239000004473 Threonine Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 235000013601 eggs Nutrition 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000003899 bactericide agent Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 235000017060 Arachis glabrata Nutrition 0.000 description 3
- 235000010777 Arachis hypogaea Nutrition 0.000 description 3
- 235000018262 Arachis monticola Nutrition 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 102000012440 Acetylcholinesterase Human genes 0.000 description 2
- 108010022752 Acetylcholinesterase Proteins 0.000 description 2
- 241000243785 Meloidogyne javanica Species 0.000 description 2
- 229940022698 acetylcholinesterase Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003987 organophosphate pesticide Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 239000005958 Fenamiphos (aka phenamiphos) Substances 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000405414 Rehmannia Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- DUEPRVBVGDRKAG-UHFFFAOYSA-N carbofuran Chemical compound CNC(=O)OC1=CC=CC2=C1OC(C)(C)C2 DUEPRVBVGDRKAG-UHFFFAOYSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- ZCJPOPBZHLUFHF-UHFFFAOYSA-N fenamiphos Chemical compound CCOP(=O)(NC(C)C)OC1=CC=C(SC)C(C)=C1 ZCJPOPBZHLUFHF-UHFFFAOYSA-N 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
- A01N41/08—Sulfonic acid halides; alpha-Hydroxy-sulfonic acids; Amino-sulfonic acids; Thiosulfonic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/26—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-nitrogen bonds
- A01N57/32—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-nitrogen bonds containing heterocyclic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
Abstract
The invention discloses a pharmaceutical composition and a pharmaceutical preparation for preventing and treating peanut root-knot nematodes and a preparation method thereof, and relates to the technical field of nematode prevention and treatment. The composition comprises the following raw materials in parts by mass: 3-7 parts of ethylicin, 1.3-1.5 parts of fosthiazate, 5-7 parts of theasaponin and 3.5-6.5 parts of ginkgo nut extract. The invention further carries out compounding with ginkgo nut extract and theasaponin components on the basis of ethylicin and fosthiazate, and the prepared medicinal preparation can realize quick and excellent poisoning effect on flower rooting and nematode, has the advantages of few raw material components and simple preparation method, and is suitable for large-scale popularization and application.
Description
Technical Field
The invention relates to the technical field of nematode control, in particular to a pharmaceutical composition and a pharmaceutical preparation for controlling peanut root-knot nematodes and a preparation method thereof.
Background
The root knot nematode disease of peanut, commonly known as rehmannia root, colony disease, dwarf yellow disease, yellow seedling disease and the like, is a disease in the world and occurs in almost all areas where peanuts are planted. The root-knot nematode disease of the peanuts can destroy the root absorption function of the peanuts, so that the plants are yellow, the fruit quantity is small, and even the plants can be harvested absolutely in severe cases.
The peanut root-knot nematode is the main pathogenic nematode for initiating the peanut root-knot nematode disease. The peanut root-knot nematode larvae invade the root tips of the peanuts to form irregular root knots, settle and take food, stimulate the proliferation of root cells, continuously damage the root tips of new roots, enable secondary roots to be agglomerated, and make nutrient absorption difficult, so that diseased plants grow slowly or are withered and not grown.
How to efficiently prevent and treat peanut root-knot nematodes is a technical problem which troubles researchers for a long time. At present, the chemical substances for preventing and controlling the peanut root-knot nematode mainly comprise fosthiazate, carbofuran, fenamiphos, thiofenamiphos and the like, but the poisoning effect of the chemical substances on the peanut root-knot nematode is not ideal, and the drug resistance of the peanut root-knot nematode to the drugs is larger.
Ethylicin is a high-efficiency broad-spectrum bactericide, has growth regulating effect and is an important raw material of compound bactericide pesticides; the fosthiazate is an organophosphorus pesticide and can inhibit the synthesis of nematode acetylcholinesterase. Although the research on compounding ethylicin and fosthiazate is related at present, the prevention and treatment effect obtained by compounding ethylicin and fosthiazate is not ideal.
Based on the current situation, the technical problem to be solved urgently is to provide a drug with good effect of preventing and controlling the flower rootage and the nematode.
Disclosure of Invention
The invention aims to provide a pharmaceutical composition and a pharmaceutical preparation for preventing and treating peanut root-knot nematode and a preparation method thereof, which are used for solving the problems in the prior art and realizing good prevention and treatment effect on the peanut root-knot nematode.
In order to achieve the purpose, the invention provides the following scheme:
one purpose of the invention is to provide a pharmaceutical composition for preventing and treating peanut root-knot nematode, which comprises the following raw materials in parts by mass:
3-7 parts of ethylicin, 1.3-1.5 parts of fosthiazate, 5-7 parts of theasaponin and 3.5-6.5 parts of ginkgo nut extract.
Further, the extraction method of the ginkgo nut extract comprises the following steps:
(1) pulverizing semen Ginkgo, reflux extracting with 75% ethanol, filtering, and concentrating under reduced pressure;
(2) dissolving the substance obtained in the step (1) in water, adding n-butanol for extraction, and concentrating and drying the n-butanol phase under reduced pressure to obtain the ginkgo nut extract.
Further, the step (1) also comprises the step of adding a preservative after the ginkgo nuts are crushed; the addition amount of the preservative is 0.2 percent of the mass of the ginkgo nuts. The preservative is threonine.
Further, the reflux extraction time is 5-7 min.
The invention also aims to provide a pharmaceutical preparation for preventing and treating peanut root-knot nematode, which contains the pharmaceutical composition.
The invention also provides a preparation method of the medicinal preparation, which comprises the following steps:
and uniformly mixing the ethylicin, the fosthiazate, the theasaponin and the ginkgo nut extract with 5-18 parts of organic solvent according to the mass part ratio, then adding 2.5-3.5 parts of emulsifier, stirring, finally adding 55-65 parts of water, and continuously and uniformly stirring to obtain the pharmaceutical preparation for preventing and treating the peanut root-knot nematode.
Further, the organic solvent is selected from one or more of ethanol, n-butanol, isobutanol or acetone.
Further, the emulsifier is ammonium dodecylbenzene sulfonate or sodium dodecylbenzene sulfonate.
Ethylicin is a high-efficiency broad-spectrum bactericide, has growth regulating effect and is an important raw material of compound bactericide pesticides. The fosthiazate is an organophosphorus pesticide and can inhibit the synthesis of nematode acetylcholinesterase.
The invention discloses the following technical effects:
the invention further carries out compounding with ginkgo nut extract and theasaponin components on the basis of ethylicin and fosthiazate, and the prepared medicinal preparation can realize quick and excellent poisoning effect on flower rooting and nematode.
According to the invention, the ginkgo biloba extract is extracted, and the complex formulation of the ginkgo biloba extract and other components has an excellent effect of poisoning peanut root-knot nematodes, while the complex formulation of the ginkgo biloba extract does not achieve an ideal effect, which may be related to different effective components of different parts of plants; the theasaponin has strong biological cell affinity, and can remarkably improve the poisoning effect of each component on nematodes.
The invention has the advantages of few raw material components and simple preparation method, and is suitable for large-scale popularization and application.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The "parts" in the present invention are all parts by mass unless otherwise specified.
Example 1
A pharmaceutical preparation for preventing and treating peanut root-knot nematodes comprises the following raw materials in parts by mass:
3 parts of ethylicin, 1.5 parts of fosthiazate, 5 parts of theasaponin and 3.5 parts of ginkgo nut extract.
The extraction method of the ginkgo nut extract comprises the following steps:
(1) pulverizing semen Ginkgo, adding threonine 0.2% of semen Ginkgo, reflux-extracting with 75% ethanol for 5min, filtering, and concentrating under reduced pressure;
(2) dissolving the substance obtained in the step (1) in water, adding n-butanol for extraction, and concentrating and drying the n-butanol phase under reduced pressure to obtain the ginkgo nut extract.
The preparation method of the pharmaceutical preparation for preventing and treating peanut root-knot nematode comprises the following steps:
uniformly mixing ethylicin, fosthiazate, theasaponin and ginkgo nut extract with 5 parts of ethanol according to the mass part ratio, then adding 2.5 parts of sodium dodecyl benzene sulfonate, stirring, finally adding 55 parts of water, and continuously and uniformly stirring to obtain the pharmaceutical preparation for preventing and treating peanut root-knot nematode.
Example 2
A pharmaceutical preparation for preventing and treating peanut root-knot nematodes comprises the following raw materials in parts by mass:
ethylicin 7 parts, fosthiazate 1.3 parts, theasaponin 6 parts and ginkgo nut extract 6.5 parts.
The extraction method of the ginkgo nut extract comprises the following steps:
(1) pulverizing semen Ginkgo, adding threonine 0.2% of semen Ginkgo, reflux-extracting with 75% ethanol for 6min, filtering, and concentrating under reduced pressure;
(2) dissolving the substance obtained in the step (1) in water, adding n-butanol for extraction, and concentrating and drying the n-butanol phase under reduced pressure to obtain the ginkgo nut extract.
The preparation method of the pharmaceutical preparation for preventing and treating peanut root-knot nematode comprises the following steps:
uniformly mixing ethylicin, fosthiazate, theasaponin and ginkgo nut extract with 18 parts of isobutanol according to the mass part ratio, then adding 3.5 parts of ammonium dodecylbenzene sulfonate, stirring, finally adding 65 parts of water, and continuously and uniformly stirring to obtain the pharmaceutical preparation for preventing and treating peanut root-knot nematodes.
Example 3
A pharmaceutical preparation for preventing and treating peanut root-knot nematodes comprises the following raw materials in parts by mass:
5 parts of ethylicin, 1.4 parts of fosthiazate, 7 parts of theasaponin and 5 parts of ginkgo nut extract.
The extraction method of the ginkgo nut extract comprises the following steps:
(1) pulverizing semen Ginkgo, adding threonine 0.2% of semen Ginkgo, reflux-extracting with 75% ethanol for 7min, filtering, and concentrating under reduced pressure;
(2) dissolving the substance obtained in the step (1) in water, adding n-butanol for extraction, and concentrating and drying the n-butanol phase under reduced pressure to obtain the ginkgo nut extract.
The preparation method of the pharmaceutical preparation for preventing and treating peanut root-knot nematode comprises the following steps:
uniformly mixing ethylicin, fosthiazate, theasaponin and ginkgo nut extract with 15 parts of acetone according to the mass part ratio, then adding 3 parts of sodium dodecyl benzene sulfonate, stirring, finally adding 60 parts of water, and continuously and uniformly stirring to obtain the pharmaceutical preparation for preventing and treating peanut root-knot nematode.
Example 4
A pharmaceutical preparation for preventing and treating peanut root-knot nematodes comprises the following raw materials in parts by mass:
6 parts of ethylicin, 1.5 parts of fosthiazate, 5 parts of theasaponin and 4 parts of ginkgo nut extract.
The extraction method of the ginkgo nut extract comprises the following steps:
(1) pulverizing semen Ginkgo, adding threonine 0.2% of semen Ginkgo, reflux-extracting with 75% ethanol for 6min, filtering, and concentrating under reduced pressure;
(2) dissolving the substance obtained in the step (1) in water, adding n-butanol for extraction, and concentrating and drying the n-butanol phase under reduced pressure to obtain the ginkgo nut extract.
The preparation method of the pharmaceutical preparation for preventing and treating peanut root-knot nematode comprises the following steps:
uniformly mixing ethylicin, fosthiazate, theasaponin and ginkgo nut extract with 10 parts of isobutanol according to the mass part ratio, then adding 2.5 parts of ammonium dodecylbenzene sulfonate, stirring, finally adding 58 parts of water, and continuously and uniformly stirring to obtain the pharmaceutical preparation for preventing and treating peanut root-knot nematodes.
Comparative example 1
The difference from example 1 is that theasaponin was not added.
Comparative example 2
The difference from example 1 is that ginkgo biloba extract was replaced with ginkgo biloba extract (same extraction method).
Comparative example 3
The difference from example 1 is that ginkgo biloba extract was not added.
Effect verification example 1:
1. test subjects: 2-instar larvae of peanut root-knot nematode.
Collecting 2-instar larvae: collecting diseased peanut strains with peanut root-knot nematode egg masses, cutting diseased roots into small sections of 2cm, rinsing in 1% sodium hypochlorite solution for about 4min, flushing and collecting nematode eggs by using 200-mesh and 500-mesh nematode sieves, diluting the nematode eggs with distilled water into 200/mL egg suspension, dropwise adding the egg suspension onto a tray paved with 2 layers of tissue paper, placing for 3d, and collecting hatching larvae.
2. The test method comprises the following steps: the test adopts an insect immersion method.
5mL of the pharmaceutical preparations of examples 1 to 4 and comparative examples 1 to 3 were added to 1kg of water, mixed, and 100 2-instar larvae of peanut root-knot nematode were added to prepare test groups 1 to 4 and control groups 1 to 3. Culturing at 25 deg.C for 4 times, treating with 0.05% Tween-80 clear water as blank control group, and counting the mortality of each group after 0.5h, 1h, and 2 h. The results are shown in Table 1.
TABLE 1
Effect test example 2
Peanut fields with serious harm of peanut root-knot nematodes and uniform physicochemical properties are used as test fields. The experimental plot was divided into 8 blocks by random averaging and used as test group 1-4, control group 1-3 and blank control group. In sowing, test groups 1-4 are applied with the pharmaceutical preparations of examples 1-4 respectively, at an application rate of 50 mL/mu, diluted with 1000 times of water; the drug preparations of comparative examples 1 to 3 were applied to control groups 1 to 3, respectively, at an application rate of 50 mL/acre, diluted with 1000 times of water and applied. The blank control group was not administered drug.
During the trial, the same conventional field management measures were used for each group.
The average density of the peanut root knot nematodes in the soil of each group after 0 th day, 3 rd day and 2 months of sowing is shown in table 2.
TABLE 2
During the test period, the peanuts in each test group grow well, the plants do not turn yellow, and the branches and leaves are stretched and healthy; the growth vigor of the control group is poor, and the plants are yellow; the blank control group has extremely poor growth potential and shows obvious symptoms of peanut root-knot nematode disease.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (8)
1. The pharmaceutical composition for preventing and treating peanut root-knot nematodes is characterized by comprising the following raw materials in parts by mass:
3-7 parts of ethylicin, 1.3-1.5 parts of fosthiazate, 5-7 parts of theasaponin and 3.5-6.5 parts of ginkgo nut extract.
2. The pharmaceutical composition as claimed in claim 1, wherein the extraction method of the ginkgo biloba extract comprises the following steps:
(1) pulverizing semen Ginkgo, reflux extracting with 75% ethanol, filtering, and concentrating under reduced pressure;
(2) dissolving the substance obtained in the step (1) in water, adding n-butanol for extraction, and concentrating and drying the n-butanol phase under reduced pressure to obtain the ginkgo nut extract.
3. The pharmaceutical composition according to claim 2, wherein the step (1) further comprises the step of adding a preservative after pulverizing the ginkgo nuts; the addition amount of the preservative is 0.2 percent of the mass of the ginkgo nuts.
4. The pharmaceutical composition of claim 2, wherein the reflux extraction time is 5-7 min.
5. A pharmaceutical preparation for controlling meloidogyne arachidis, comprising the pharmaceutical composition according to any one of claims 1 to 4.
6. The method of preparing a pharmaceutical formulation according to claim 5, comprising the steps of:
and uniformly mixing the ethylicin, the fosthiazate, the theasaponin and the ginkgo nut extract with 5-18 parts of organic solvent according to the mass part ratio, then adding 2.5-3.5 parts of emulsifier, stirring, finally adding 55-65 parts of water, and continuously and uniformly stirring to obtain the pharmaceutical preparation for preventing and treating the peanut root-knot nematode.
7. The method according to claim 6, wherein the organic solvent is selected from one or more of ethanol, n-butanol, isobutanol, and acetone.
8. The method according to claim 6, wherein the emulsifier is ammonium dodecylbenzene sulfonate or sodium dodecylbenzene sulfonate.
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