CN107593726B - Insecticidal composition containing dazomet and cartap and application thereof - Google Patents
Insecticidal composition containing dazomet and cartap and application thereof Download PDFInfo
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Abstract
The invention discloses a pesticide composition containing dazomet and cartap and application thereof. The insecticidal composition contains the dazomet and cartap as main active ingredients, and the mass ratio of the dazomet to the cartap is 30:1-1: 20. The insecticidal composition can be applied to prevention and removal of root-knot nematodes on cucumbers, tomatoes, hot peppers, peanuts, sweet potatoes, forestry flower nurseries, rice or wheat.
Description
Technical Field
The invention belongs to the technical field of pesticide preparations, and relates to an insecticidal composition containing dazomet and cartap and application thereof.
Background
Dazomet is an environment-friendly broad-spectrum comprehensive soil fumigation disinfectant with high efficiency, low toxicity and no residue. When the pesticide is applied to moist soil, the pesticide is decomposed into toxic methyl isothiocyanate, formaldehyde, hydrogen sulfide and the like in the soil, and the toxic methyl isothiocyanate, the formaldehyde, the hydrogen sulfide and the like are quickly diffused among soil particles, so that various nematodes, pathogenic bacteria, underground pests and germinated weed seeds in the soil are effectively killed, and the effect of cleaning the soil is achieved.
Cartap, also called cartap and paidan, belongs to a moderate toxicity pesticide. It has no allergic reaction to eyes and skin under normal conditions. Has no carcinogenic, teratogenic and mutagenic effects. It is toxic to fish, bee and domestic silkworm, low-toxic to birds and no-toxic to natural enemies such as spider. Cartap has strong stomach toxicity, and has the functions of contact poisoning, certain food refusal, egg killing and the like. Quick effect on pests, long residual action period and broad spectrum. The cartap can be used for preventing and treating various pests and nematodes of Lepidoptera, Coleoptera, Hemiptera, Diptera, etc.
Root-knot nematodes are highly specialized species of xenotrophic plant pathogenic nematodes. The nematodes known as harmful to vegetables mainly include high bowknot nematodes, peanut root knot nematodes, northern root knot nematodes, southern root knot nematodes, javanica root knot nematodes, beet root knot nematodes and the like. The nematode host range is wide, which causes serious loss to most of food crops, oil crops, fiber crops, tobacco, tea, fruit trees, vegetables, medicinal materials, flowers and the like, and can also spread some fungi and bacterial diseases. Root-knot nematodes are mainly harmful to the roots of various plants, and are characterized by a more normal increase in lateral roots and fibrous roots and the formation of spherical or conical white nodules of various sizes, some in the form of moniliforme, on the fibrous roots of young roots. The overground part of the damaged plant grows short and slow, the color of the leaves is abnormal, the fruiting is less, the yield is low, and even the plant dies in advance.
At present, no research report and patent application of the composition of the dazomet and the cartap for preventing and treating the root-knot nematode exists in China, and no related pesticide registration and product sale exists.
Disclosure of Invention
The invention aims to provide an insecticidal composition containing dazomet and cartap against the defects of the prior art.
Still another object of the present invention is to provide the use of the pesticidal composition.
The insecticidal composition contains the dazomet and cartap as main active ingredients, and the mass ratio of the dazomet to the cartap is 30:1-1: 20.
The mass ratio of the dazomet to the cartap in the insecticidal composition is preferably 10:1-3: 1.
In some preferred embodiments: the dazomet and cartap can be selected from the proportion of 30:1, 20:1, 10:1, 5:1, 3:1, 1:3, 1:5, 1:10 and 1:20, and can generate synergistic insecticidal action.
In the insecticidal composition, the total mass percentage of the dazomet and the cartap is preferably 2-90%, and further preferably 40-90%.
The insecticidal composition is preferably prepared into any one dosage form by taking the dazomet and cartap as main active ingredients and auxiliary materials allowed by pesticides.
The dosage form is preferably wettable powder, water dispersible granules and broadcast granules; the auxiliary materials are preferably one or more of a wetting agent, a dispersing agent, a filler, a disintegrating agent and a binder.
As further preferences for the pesticidal composition of the invention: the wetting agent is selected from one or more of sodium dodecyl sulfate or nekal; the dispersant is one or more selected from sodium lignosulfonate, phosphate polyoxyethylene ether, polycarboxylate (such as GY-D800, GY-D900 and T-36) and alkyl naphthalene sulfonate; the filler is selected from one or more of kaolin, starch, talcum powder, white carbon black, diatomite, bentonite or montmorillonite; the disintegrant is selected from one or more of ammonium sulfate or sodium sulfate; the binder is selected from one or more of polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 4000, polyethylene glycol 6000, polyvinyl alcohol 1788 or povidone K30.
When the dosage form is wettable powder, the auxiliary materials are a wetting agent, a dispersing agent and a filling material; the preparation method comprises the following steps: adding the filler, the dazomet, the cartap, the wetting agent and the dispersing agent into a stirring device, uniformly stirring, and then crushing into 325-800 meshes by using an airflow crusher.
When the dosage form is water dispersible granules, the auxiliary materials are wetting agents, dispersing agents, disintegrating agents and fillers; the preparation method comprises the following steps: adding a filler, a disintegrating agent, dazomet, cartap, a wetting agent and a dispersing agent into a stirring device, uniformly stirring, then crushing into over 1250 meshes by using a jet mill, adding water into the obtained powder material, kneading, preparing into cylindrical granules with the diameter of 0.5mm or 0.8mm by using a rotary extrusion granulator, and drying and screening to obtain the water dispersible granules;
when the formulation is a broadcast granule, the auxiliary materials are adhesive and filler; the preparation method comprises the following steps: adding the dazomet, the cartap, the binder and the filler into a stirring device, uniformly stirring, and extruding and granulating by using double rollers to obtain the broadcast granule with the particle size of 2-6 mm.
The invention relates to application of an insecticidal composition in preventing and removing root-knot nematodes.
The insecticidal composition is preferably applied to control of root-knot nematodes on cucumbers, tomatoes, hot peppers, peanuts, sweet potatoes, forestry flower nurseries, rice or wheat.
Has the advantages that:
the dazomet and cartap have different action mechanisms, and the dazomet has the effects of killing nematodes, killing bacteria, killing underground pests and inhibiting weed seed germination; cartap has strong stomach toxicity, and has the functions of contact poisoning, certain food refusal, egg killing and the like. The two are compounded according to a specific mixture ratio range, so that the synergistic effect is generated on the prevention and the treatment of the root-knot nematode, the resistance of pests to pesticides can be effectively delayed, and the prevention and treatment object range is enlarged. The two pesticides are compounded for broadcasting and mixing with soil or by adopting a water-following pesticide application method such as irrigating the roots of crops, coarse spraying on the ground, flood flooding and the like, and can prevent and control nematodes and underground pests, soil fungi, weeds and aerial parts of pests of crops.
The two pesticides are compounded for broadcasting and mixing with soil or by adopting a water-following pesticide application method of irrigating crop roots, rough spraying on the ground, flood flooding and the like, are used for preventing and treating root-knot nematodes, underground pests, soil diseases and weeds, and reduce the overground part of pests of crops.
Detailed Description
Example 1
A62% dazomet and cartap pesticide composition is wettable powder, and the wettable powder comprises the following components in percentage by weight: 60kg of dazomet, 2kg of cartap, 2kg of sodium dodecyl sulfate, 5kg of methylene dinaphthalene sodium sulfonate and the balance of kaolin to 100 kg.
The preparation method of the wettable powder comprises the steps of sequentially adding kaolin, dazomet, cartap, sodium dodecyl sulfate and methylene dinaphthalene sulfonate into a stirring device, uniformly stirring, and then crushing into 325 meshes by using an air flow crusher to obtain the wettable powder.
Example 2
A pesticide composition of 42% dazomet and cartap is wettable powder, and comprises the following components in percentage by weight: 40kg of dazomet, 2kg of cartap, 1kg of sodium dodecyl sulfate, 6kg of sodium lignosulfonate, 2kg of white carbon black and kaolin which are complemented to 100 kg.
The preparation method of the wettable powder comprises the steps of sequentially adding kaolin, dazomet, cartap, sodium dodecyl sulfate, sodium lignosulfonate and white carbon black into a stirring device, uniformly stirring, and then crushing into 325 meshes by an air flow crusher to obtain the wettable powder.
Example 3
A pesticide composition of 80% dazomet and cartap is wettable powder, and comprises the following components in percentage by weight: 20kg of dazomet, 60kg of cartap, 1kg of nekal BX, 8kg of sodium lignosulfonate, 2kg of white carbon black and the balance of diatomite to 100 kg.
The preparation method of the wettable powder comprises the steps of sequentially adding diatomite, dazomet, cartap, nekal BX, sodium lignosulfonate and white carbon black into a stirring device, uniformly stirring, and then crushing into 325 meshes by an airflow crusher to obtain the wettable powder.
Example 4
A pesticide composition of 84% dazomet and cartap is a water dispersible granule, and comprises the following components in percentage by weight: 70kg of dazomet, 14kg of cartap, 2kg of sodium dodecyl sulfate, GY-D8004 kg, 5kg of sodium sulfate and the balance of montmorillonite to 100 kg.
The preparation method of the water dispersible granule comprises the steps of sequentially adding montmorillonite, sodium sulfate, dazomet, cartap, sodium dodecyl sulfate and GY-D800 into a stirring device, uniformly stirring, then crushing into 1250 meshes by an airflow crusher, adding water into the obtained powder material for kneading, preparing into cylindrical granules with the diameter of 0.5 or 0.8mm by using a rotary extrusion granulator, and drying and screening to obtain the water dispersible granule.
Example 5
A pesticide composition of 44% of dazomet and cartap is a water dispersible granule, and comprises the following components in percentage by weight: 40kg of dazomet, 4kg of cartap, 1kg of sodium dodecyl sulfate, GY-D9004 kg, 15kg of ammonium sulfate and the balance of starch to 100 kg.
The preparation method of the water dispersible granule comprises the steps of sequentially adding starch, ammonium sulfate, dazomet, cartap, sodium dodecyl sulfate and GY-D900 into a stirring device, uniformly stirring, then crushing into 1250 meshes by an airflow crusher, adding water into the obtained powder material, kneading, preparing into cylindrical granules with the diameter of 0.5 or 0.8mm by using a rotary extrusion granulator, and drying and screening to obtain the water dispersible granule.
Example 6
A pesticide composition of 24% of dazomet and cartap is a water dispersible granule, and comprises the following components in percentage by weight: 4kg of dazomet, 20kg of cartap, 1kg of sodium dodecyl sulfate, 4kg of T-36 (polycarboxylate dispersant, produced by Solvay company) and 20kg of sodium sulfate, and the balance of kaolin is up to 100 kg.
The preparation method of the water dispersible granule comprises the steps of sequentially adding kaolin, sodium sulfate, dazomet, cartap, sodium dodecyl sulfate and T-36 into a stirring device, uniformly stirring, crushing into 1250 meshes by an airflow crusher, adding water into the obtained powder material, kneading, preparing into cylindrical granules with the diameter of 0.5 or 0.8mm by using a rotary extrusion granulator, drying and screening to obtain the water dispersible granule
Example 7
A pesticide composition of 22% of dazomet and cartap is a spreading granule, and the wettable powder spreading granule comprises the following components in percentage by weight: 2kg of dazomet, 20kg of cartap, 6002kg of polyethylene glycol and montmorillonite, wherein the weight of the mixture is up to 100 kg.
Adding the dazomet, cartap, polyethylene glycol 600 and montmorillonite into a stirring device, uniformly stirring, and extruding a granulator by using double rollers to obtain the broadcast granules with the particle size of 4.5 mm.
Example 8
A pesticide composition of 40% of dazomet and cartap is a spreading granule, and the wettable powder spreading granule comprises the following components in percentage by weight: 20kg of dazomet, 20kg of cartap, 6002kg of polyethylene glycol and kaolin, wherein the balance is 100 kg.
Adding the dazomet, cartap, polyethylene glycol 600 and kaolin into a stirring device, uniformly stirring, and extruding a granulator by using double rollers to obtain the broadcast granules with the particle size of 4.5 mm.
Example 9
A pesticide composition of 42% dazomet and cartap is a spreading granule, and the wettable powder spreading granule comprises the following components in percentage by weight: 2kg of dazomet, 40kg of cartap, 17882kg of polyvinyl alcohol and the balance of talcum powder to 100 kg.
Adding the dazomet, cartap, polyvinyl alcohol 1788 and talcum powder into a stirring device, uniformly stirring, and extruding a granulator by using double rollers to obtain the broadcast granules with the particle size of 4.5 mm.
Example 10
The insecticidal effect of the compound of the dazomet and the cartap is tested, and the specific implementation mode is as follows:
and (3) testing pesticides: 62% of a dazomet cartap wettable powder (example 1), 42% of a dazomet cartap wettable powder (example 2), 80% of a dazomet cartap wettable powder (example 3), 84% of a dazomet cartap water dispersible granule (example 4), 44% of a dazomet cartap water dispersible granule (example 5), 24% of a dazomet cartap water dispersible granule (example 6) and 22% of a dazomet cartap broadcasting granule (example 7); the granules used for the application of 40% of dazomet cartap (example 8) and the granules used for the application of 42% of dazomet cartap (example 9).
Control agents: 98% dazomet microgranule
Object for controlling cucumber root-knot nematode
Environmental conditions: the test is set in Hongze county of Huaian city, the conditions of test cell cultivation, fertilizer and water management and the like are consistent, and the cucumber cultivar is Zhongnong No. 106.
And (3) experimental design: the experiment had a total of 11 treatments, each treatment was repeated 4 times for a total of 32 cells, each cell had an area of 20 square meters, and the cells were randomly arranged. The 11 treatments were: (1) 62% of dazomet cartap wettable powder, (2) 42% of dazomet cartap wettable powder, (3) 80% of dazomet cartap wettable powder, (4) 84% of dazomet cartap water dispersible granules, (5) 44% of dazomet cartap water dispersible granules, (6) 24% of dazomet cartap water dispersible granules, (7) 22% of dazomet cartap broadcasting granules, (8) 40% of dazomet cartap broadcasting granules, (9) 42% of dazomet cartap broadcasting granules, (10) 98% of dazomet microgranules, and (11) clear water contrast.
The test method comprises the following steps: selecting plots with evenly distributed root-knot nematodes and medium and heavy weights for testing. Medicament treatment: after cleaning a field garden, applying required fertilizer, turning over, leveling the land, watering, applying the fertilizer when the soil humidity reaches 60% -70%, uniformly spreading the agent in each cell according to the corresponding dose, then carrying out rotary tillage with a rotary tillage plow to the depth of about 25cm, enabling the agent to be in comprehensive and uniform contact with soil of a plough layer, then laminating, after 20 days, uncovering a plastic film, opening the air, and after 30 days, planting cucumbers.
The investigation method comprises the following steps: after 90 days of application, randomly investigating 15 cucumbers in each cell, digging out roots, recording the number of disease plants at each level, calculating the disease plant rate, disease index and prevention and treatment effect, carrying out variance analysis on the prevention effect, and comparing the difference significance among treatments by using a new repeated polarization method. The grading method of diseased plants comprises the following steps:
grading the disease condition according to the following standard; calculating disease index and relative prevention effect according to a formula.
Grade 0 indicates complete root system without root knots.
Grade 1 is a few root nodules with root necrosis less than 25%.
The grade 2 is that the number of root knots accounts for 26-50% of the root coefficient.
The 3 grade is that the root knot number accounts for 51 to 75 percent of the root coefficient.
The 4 grade is that the root knots are extremely large and large, and account for 76% -100% of the root coefficient.
Disease index ∑ (number of plants at each stage × number of the stage)/(total number of plants × 4) × 100.
The preventing and treating effect (%) is (contrast disease index-treatment disease index)/contrast disease index is multiplied by 100
The results are shown in Table 1.
TABLE 1 prevention and treatment of cucumber root-knot nematode with test agents
| Treatment of | Dosage (a.i. g/m)2) | Index of disease condition | Control effect (%) |
| 62% dazomet cartap wettable powder | 40 | 9.40 | 83.01d |
| Wettable 42% dazomet cartap powder | 40 | 8.19 | 85.20c |
| 80% dazomet cartap wettable powder | 40 | 9.87 | 82.16de |
| 84% dazomet cartap water dispersible granule | 40 | 6.72 | 87.86b |
| 44% dazomet cartap water dispersible granule | 40 | 4.80 | 91.33a |
| 24% dazomet cartap water dispersible granule | 40 | 10.41 | 81.19e |
| Broadcast application granule of 22% dazomet cartap | 40 | 11.81 | 78.66f |
| 40% dazomet cartap spreading granule | 40 | 8.40 | 84.82c |
| 42% dazomet cartap spreading granule | 40 | 13.50 | 75.60g |
| 98% dazomet microgranule | 40 | 15.41 | 72.15h |
| 50% cartap soluble powder | 40 | 25.93 | 53.66i |
| Blank control | 0 | 55.31 | / |
Note: lower case letters indicate a 5% significance level, compared using Duncan's new repolarization method in DPS software.
The test result shows that the control effect of the compound of the two components of the dazomet and the cartap is obviously higher than that of the single dose of the dazomet and the cartap. Therefore, the dazomet and the cartap are used together in the production practice, so that the method is economical, efficient and environment-friendly.
Example 11
Southern root knot nematodes, collected in cucumber greenhouses in Hongze, Huai' an, were prepared by picking fresh oocysts from damaged cucumber root knots and placing them on filter paper, placing the filter paper on a 75 μm mesh screen, and placing the mesh screen in a petri dish containing clear water so that the water slightly overflowed the egg mass. Incubating at 25 deg.C in incubator, collecting 2-instar larvae in culture dish every 24 hr, and placing in 4 deg.C refrigerator for use.
Medicament: 98% of dazomet raw medicine and 98% of cartap raw medicine
Determination of toxicity of medicament to meloidogyne incognita
Dissolving 98% dazomet raw medicine with acetone to prepare a mother solution of 1000mg/L, and dissolving 98% cartap raw medicine with sterilized water to obtain a mother solution of 10000 mug/mL; the compound mixture of the dazomet and the cartap is prepared by the dazomet and cartap mother liquor, wherein the dazomet: the cartap proportion is respectively as follows: 30:1, 20:1, 10:1, 5:1, 3:1, 1:3, 1:5, 1:10, 1: 20. The prepared drug was diluted with sterile water to a series of concentrations, 5 concentrations were set for each experiment, and treated with clear water as a control. Preparing the 2-year-old root-knot nematodes obtained by separation and activation into 200 per mL suspension, and placing 0.5mL of test solution in each zone in a small hole by adopting a direct contact killing method and taking a biochemical test plate with 24 holes. Equal volumes of nematode suspension were added and equal amounts of clear water were added to the blank, with 3 replicates per treatment set-up. Sealing the 24-hole plate with sealing film, placing in 25 deg.C incubator for 48 hr, pouring the treated and control nematode suspensions into 500-mesh sieve, filtering to remove medicinal liquid, examining survival number and death number of root-knot nematodes, and calculating and correcting mortality.
Using DPS software to process data and calculate regression equation and LC50. Combination assay of the cocktail cotoxicity Coefficient (CTC, Co-toxin coeffient) was calculated according to the method described in Sun Yunpei and Johnson (1960). Judging the combined virulence of the medicament by using the CTC value, wherein the CTC is less than or equal to 80 and shows antagonism; 80 < CTC < 120 as additive effect; CTC > 120 shows synergistic effect.
The test results are shown in Table 2
TABLE 2
As can be seen from table 2, the ratio of dazomet to cartap was 30:1-1: the insecticidal effect of the 20-range compound pairing Meloidogyne incognita Chitwood is obviously improved, which shows that the two compound combinations have obvious synergistic effect on preventing and killing Meloidogyne incognita Chitwood.
The above embodiments are only for explaining and illustrating the technical solution of the present invention, but not for limiting the present invention. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (9)
1. The insecticidal composition containing the dazomet and the cartap is characterized by taking the dazomet and the cartap as effective components, wherein the mass ratio of the dazomet to the cartap is 30:1-1: 20.
2. The insecticidal composition according to claim 1, wherein the mass ratio of the dazomet to the cartap in the insecticidal composition is 10:1-1: 5.
3. The insecticidal composition according to claim 1 or 2, characterized in that the total mass percentage of dazomet and cartap in the insecticidal composition is 2-90%.
4. The insecticidal composition according to claim 1, characterized in that the insecticidal composition is prepared into any one of dosage forms by taking dazomet and cartap as active ingredients and auxiliary materials allowed by pesticides.
5. The insecticidal composition according to claim 4, wherein the formulation is wettable powder, water dispersible granule, broadcast granule; the auxiliary materials are selected from one or more of wetting agents, dispersing agents, fillers, disintegrating agents and binding agents.
6. The insecticidal composition according to claim 5, characterized in that the wetting agent is selected from one or more of sodium lauryl sulfate or nekal; the dispersing agent is selected from one or more of sodium lignosulfonate, phosphate polyoxyethylene ether, polycarboxylate and alkyl naphthalene sulfonate; the filler is selected from one or more of kaolin, starch, talcum powder, white carbon black, diatomite, bentonite or montmorillonite; the disintegrant is selected from one or more of ammonium sulfate or sodium sulfate; the binder is selected from one or more of polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 4000, polyethylene glycol 6000, polyvinyl alcohol 1788 or povidone K30.
7. The insecticidal composition according to claim 6, wherein when the formulation is wettable powder, the auxiliary materials are wetting agent, dispersing agent and filler; the preparation method comprises adding filler, dazomet, cartap, wetting agent and dispersant into a stirring device, uniformly stirring, and pulverizing to 325-800 meshes by a jet mill;
when the dosage form is water dispersible granules, the auxiliary materials are wetting agents, dispersing agents, disintegrating agents and fillers; the preparation method comprises the following steps: adding a filler, a disintegrating agent, dazomet, cartap, a wetting agent and a dispersing agent into a stirring device, uniformly stirring, then crushing into 800-;
when the formulation is a broadcast granule, the auxiliary materials are adhesive and filler; the preparation method comprises the following steps: adding the dazomet, the cartap, the binder and the filler into a stirring device, uniformly stirring, and extruding a granulator by using double rollers to obtain the broadcast granules with the particle size of 2-6 mm.
8. Use of the insecticidal composition according to any one of claims 1 to 7 for the control of root-knot nematodes.
9. The use according to claim 8, characterized in that the pesticidal composition is used for controlling root knot nematodes on cucumber, tomato, pepper, peanut, sweet potato, forestry flower nursery, rice or wheat.
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| CN102258053A (en) * | 2011-06-01 | 2011-11-30 | 海利尔药业集团股份有限公司 | Agricultural chemical composition |
| CN102652511A (en) * | 2011-03-01 | 2012-09-05 | 中国农业科学院植物保护研究所 | Mixed application technology for controlling root-knot nematodes, soil-borne pathogenic fungi, and weeds |
| CN104082319A (en) * | 2014-07-08 | 2014-10-08 | 江苏省绿盾植保农药实验有限公司 | Method for controlling black eye-spot of rice grains by soaking seeds |
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| CN102652511A (en) * | 2011-03-01 | 2012-09-05 | 中国农业科学院植物保护研究所 | Mixed application technology for controlling root-knot nematodes, soil-borne pathogenic fungi, and weeds |
| CN102258053A (en) * | 2011-06-01 | 2011-11-30 | 海利尔药业集团股份有限公司 | Agricultural chemical composition |
| CN104082319A (en) * | 2014-07-08 | 2014-10-08 | 江苏省绿盾植保农药实验有限公司 | Method for controlling black eye-spot of rice grains by soaking seeds |
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