CN102613256A - Plant medicine of tobacco root knot nematode disease and preparation method thereof - Google Patents
Plant medicine of tobacco root knot nematode disease and preparation method thereof Download PDFInfo
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- CN102613256A CN102613256A CN2012100501114A CN201210050111A CN102613256A CN 102613256 A CN102613256 A CN 102613256A CN 2012100501114 A CN2012100501114 A CN 2012100501114A CN 201210050111 A CN201210050111 A CN 201210050111A CN 102613256 A CN102613256 A CN 102613256A
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Abstract
The invention discloses plant medicine of tobacco root knot nematode disease and a preparation method thereof. The medicine comprises the following bulk drugs as per parts by weight: 35 to 45 parts of marigold root, 16 to 20 parts of ginkgo biloba sarcotesta and 9 to 11 parts of pepper. The preparation method comprises the steps of primary crushing, alcohol extracting, ultrasonic cell crushing and extracting, concentrating and the like. The plant medicine and the preparation method adopt plant material with insecticidal action and the effective extracting method, so that the medicine is safe and environmentally friendly, and can effectively kill and restrain root-knot nematode harming tobacco.
Description
[technical field]
The present invention relates to insecticide, relate in particular to the plant medical agent of root knot nematode disease evil and preparation method thereof that grows tobacco.
[background technology]
The tobacco root knot nematode disease takes place comparatively general in China in recent years; Economize investigation according to planting cigarette to 16; Except that northeast 3 provinces had not found that root-knot nematode is caused harm, all the other 13 provinces all had root-knot nematode to cause harm, and are on the rise; The trend that area has to be increased year by year takes place, and has become one of the main disease of tobacco production that causes harm.According to incompletely statistics: these diseases of province such as 1989-1991 is only in Anhui, Henan, Sichuan, Guizhou, Zhejiang, Yunnan cause every year on average nearly 6,000 ten thousand yuan of economic loss.The investigation of recent years shows, cause harm in China cigarette district area and degree of tobacco root knot nematode disease all has the trend of increase, and its scope that causes harm enlarges year by year.Root-knot nematode also can be caused wound to bring out other rhizome portion diseases at cigarette strain root because of nematode and take place except that the cigarette strain of directly causing harm, and like black shank, root black rot, bacterial wilt etc., making causes harm increases the weight of.Owing to produce to go up lack resistant variety, cropping index is high, and the use of chemical nematode killing agent such as is restricted at reason, therefore needs to pay attention to the control of this disease.
At present, the root-knot nematode disease control be main with chemical control, other prevent and treat the improvement strategy of methods for replenishing.The general kind of chemistry nematode killing agent is single, toxicity big, Environmental compatibility is poor, nematode is easy to generate resistance, more and more can not adapt to grain security and requirements of green environmental protection.Breeding for disease resistance is the method for a kind of economy, effectively preventing root-knot nematode, but the resistance of some resistant varieties is single at present, is prone to make the microspecies of nematode to morph, and the resistant material scarcity has satisfied not present demand.Though biopesticide toxicity is low, little to receive such environmental effects very big to environmental disruption, and drug effect is unstable, and destroys soil microbial community easily.Plant resource nematode killing agent control plant nematode diseases Environmental Safety is the research focus of present plant nematode diseases control.
Botanical pesticide is meant the metabolite of the biologically active that from plant, extracts, and that the novel agrochemical that is processed into has is nuisanceless, pollution-free, noresidue and be difficult for producing advantage such as resistance.
[summary of the invention]
The technical problem that the present invention will solve provide one grow tobacco root knot nematode disease evil plant medical agent.
The another one technical problem that the present invention will solve provide one grow tobacco root knot nematode disease evil the preparation method of plant medical agent.
For the plant medical agent of tobacco root-knot nematode disease, the technical scheme that the present invention adopts is to be made up of following bulk drugs: Aztec marigold root 35-45 part, gingko episperm 16-20 part, Chinese prickly ash 9-11 part.
For the preparation method of the plant medical agent of tobacco root-knot nematode disease, the technical scheme that the present invention adopts is may further comprise the steps:
(1) the preliminary pulverizing takes by weighing the bulk drug of respectively distinguishing the flavor of by formula rate, is ground into meal, mixing earlier;
(2) alcohol extract takes by weighing the said medicine meal in extractor, adds alcohol reflux and extracts, and obtains filtrating after the filtration;
(3) ultrasonic cell pulverization is got the dregs of a decoction after the alcohol extracting, puts into ultrasonic cell pulverization extractor, and adding distil water carries out water and carries; Behind ultrasonic the finishing, obtain filtrating after the filtration;
(4) concentrate all filtratings that above-mentioned alcohol extracting and water are carried and merge, wave most ethanol, high speed centrifugation, it is concentrated to get supernatant, and packing is subsequent use.
Medical mechanism:
Aztec marigold is commonly called as tagetes erecta, marigold, and the composite family Tagetes is 1 year sward basis, is the ornamental plant that is distributed widely in all over the world.Aztec marigold root extract can suppress the mushroom disease of crops, vegetables etc., and wherein alkaloids substance accounts for greatly.
Gingko episperm is the pulp of seed duricrust outside, in the ginkgo related industry is produced, is regarded as refuse for a long time and abandons, and gingko episperm has many values in fact, can turn waste into wealth.Gingko episperm mainly contains phenolic acid class, general flavone, lactone, 4 big types of tens kinds of organic compounds of polysaccharose through the composition of isolation identification.The highest with phenolic acid compound content, account for 65% of extract.Has mainly also compounds for this reason of the active active ingredient of killing pests and suppressing bacteria.
Chinese prickly ash is the dry mature skin of Rutaceae shrub or dungarunga plant green pepper, and is generally ripe before and after the beginning of autumn.Originate in provinces such as Sichuan, Shaanxi, Henan, Hebei, Shanxi, Yunnan, the quality of producing with Sichuan is good, is height with Hebei, Shanxi output.Chinese prickly ash is Chinese distinctive spices, ranks first of the condiment " 13 perfume (or spice) ".Pericarp contains volatile oil, and main body of oil is citrene, cumic alcohol, geraniol.In addition and contain multiple compounds such as phytosterol and unsaturated organic acid.The document record, volatile oil has bacteriostasis and anthelmintic action in the Chinese prickly ash pericarp.
The invention has the beneficial effects as follows:
Employing has the medicinal plant raw material of insecticidal action, is aided with effective method for distilling, and the root-knot nematode of tobacco can killed and suppress to endanger to the pharmacy security environmental protection of processing effectively.
[embodiment]
Embodiment 1:
One grow tobacco root knot nematode disease evil plant medical agent, its raw material components and weight portion are: 35 parts of Aztec marigolds, 16 parts of gingko episperms, 9 parts in Chinese prickly ash.
The preparation method is following:
(1) the preliminary pulverizing takes by weighing the traditional Chinese medicine of respectively distinguishing the flavor of by formula rate, is ground into meal, mixing earlier;
(2) alcohol extract takes by weighing the said medicine meal in extractor, adds alcohol reflux and extracts, and extraction conditions is: 70 ℃, 60% alcohol reflux of 7 times of volumes extracts 4 times, each 1h; Obtain filtrating after the filtration;
(3) ultrasonic cell pulverization is got the dregs of a decoction after the alcohol extracting, puts into ultrasonic cell pulverization extractor, adds 8 times of amount distilled water and carries out water and carry; Behind ultrasonic the finishing, obtain filtrating after the filtration;
(4) concentrate all filtratings that above-mentioned alcohol extracting and water are carried and merge, wave most ethanol, high speed centrifugation, it is concentrated to get supernatant, and packing is subsequent use.
Embodiment 2:
One grow tobacco root knot nematode disease evil plant medical agent, its raw material components and weight portion are: 40 parts of Aztec marigolds, 18 parts of gingko episperms, 10 parts in Chinese prickly ash.
The preparation method is with embodiment 1.
Embodiment 3:
One grow tobacco root knot nematode disease evil plant medical agent, its raw material components and weight portion are: 45 parts of Aztec marigolds, 20 parts of gingko episperms, 11 parts in Chinese prickly ash.
The preparation method is with embodiment 1.
Above content only is to what how the present invention implemented to be done to give an example and explanation; Rather than restriction protection scope of the present invention; Under the technical staff in present technique field described specific embodiment is made various modifications or replenish or adopt similar mode to substitute; Only otherwise depart from the structure of invention or surmount the defined scope of these claims, all should belong to protection scope of the present invention.
Claims (2)
- One grow tobacco root knot nematode disease evil plant medical agent, it is characterized in that, form by following bulk drugs: Aztec marigold root 35-45 part, gingko episperm 16-20 part, Chinese prickly ash 9-11 part.
- 2. according to the preparation method of the said plant medical agent of claim 1, it is characterized in that, may further comprise the steps:(1) the preliminary pulverizing takes by weighing the bulk drug of respectively distinguishing the flavor of by formula rate, is ground into meal, mixing earlier;(2) alcohol extract takes by weighing the said medicine meal in extractor, adds alcohol reflux and extracts, and obtains filtrating after the filtration;(3) ultrasonic cell pulverization is got the dregs of a decoction after the alcohol extracting, puts into ultrasonic cell pulverization extractor, and adding distil water carries out water and carries; Behind ultrasonic the finishing, obtain filtrating after the filtration;(4) concentrate all filtratings that above-mentioned alcohol extracting and water are carried and merge, wave most ethanol, high speed centrifugation, it is concentrated to get supernatant, and packing is subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012100501114A CN102613256A (en) | 2012-02-24 | 2012-02-24 | Plant medicine of tobacco root knot nematode disease and preparation method thereof |
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| CN2012100501114A CN102613256A (en) | 2012-02-24 | 2012-02-24 | Plant medicine of tobacco root knot nematode disease and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004401A (en) * | 2012-11-27 | 2013-04-03 | 天津滨海国际花卉科技园区股份有限公司 | Turfgrass nematode control method |
| CN103039536A (en) * | 2012-12-27 | 2013-04-17 | 广东中迅农科股份有限公司 | Pesticide composition with functions of preventing and controlling nematodes |
| CN104430557A (en) * | 2014-11-15 | 2015-03-25 | 辽宁农业职业技术学院 | Chinese herbal medicine composition for preventing and treating plant root-knot nematode as well as preparation method and application thereof |
| CN104920124A (en) * | 2015-06-19 | 2015-09-23 | 云南绿叶生防科技有限公司 | Method for inhibiting tobacco root-knot nematode disease through janapan |
| CN105027875A (en) * | 2015-06-19 | 2015-11-11 | 四川省烟草公司攀枝花市公司（攀枝花市烟草公司） | Method for preventing and controlling tobacco root-knot nematodes |
| CN106106544A (en) * | 2016-06-27 | 2016-11-16 | 武汉维斯康生物科技有限公司 | The water-soluble fertilizer insecticidal additive of Citrus root-knot nematode, water-soluble fertilizer and preparation method thereof and using method can be killed |
| CN106867542A (en) * | 2015-12-14 | 2017-06-20 | 灵武市森保科技开发有限公司 | A kind of facilities vegetable continuous cropping soil conditioner and preparation method thereof |
| CN106856948A (en) * | 2017-03-15 | 2017-06-20 | 湖北省烟草科学研究院 | A kind of field planting method for preventing and treating tobacco bacterial wilt |
| CN113994960A (en) * | 2021-12-01 | 2022-02-01 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and pharmaceutical preparation for preventing and treating peanut root-knot nematode and preparation method thereof |
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2012
- 2012-02-24 CN CN2012100501114A patent/CN102613256A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004401A (en) * | 2012-11-27 | 2013-04-03 | 天津滨海国际花卉科技园区股份有限公司 | Turfgrass nematode control method |
| CN103039536A (en) * | 2012-12-27 | 2013-04-17 | 广东中迅农科股份有限公司 | Pesticide composition with functions of preventing and controlling nematodes |
| CN103039536B (en) * | 2012-12-27 | 2014-12-31 | 广东中迅农科股份有限公司 | Pesticide composition with functions of preventing and controlling nematodes |
| CN104430557A (en) * | 2014-11-15 | 2015-03-25 | 辽宁农业职业技术学院 | Chinese herbal medicine composition for preventing and treating plant root-knot nematode as well as preparation method and application thereof |
| CN104920124A (en) * | 2015-06-19 | 2015-09-23 | 云南绿叶生防科技有限公司 | Method for inhibiting tobacco root-knot nematode disease through janapan |
| CN105027875A (en) * | 2015-06-19 | 2015-11-11 | 四川省烟草公司攀枝花市公司（攀枝花市烟草公司） | Method for preventing and controlling tobacco root-knot nematodes |
| CN106867542A (en) * | 2015-12-14 | 2017-06-20 | 灵武市森保科技开发有限公司 | A kind of facilities vegetable continuous cropping soil conditioner and preparation method thereof |
| CN106867542B (en) * | 2015-12-14 | 2020-04-21 | 灵武市森保科技开发有限公司 | Soil conditioner for facility vegetable continuous cropping and preparation method thereof |
| CN106106544A (en) * | 2016-06-27 | 2016-11-16 | 武汉维斯康生物科技有限公司 | The water-soluble fertilizer insecticidal additive of Citrus root-knot nematode, water-soluble fertilizer and preparation method thereof and using method can be killed |
| CN106856948A (en) * | 2017-03-15 | 2017-06-20 | 湖北省烟草科学研究院 | A kind of field planting method for preventing and treating tobacco bacterial wilt |
| CN113994960A (en) * | 2021-12-01 | 2022-02-01 | 江西省农业科学院植物保护研究所 | Pharmaceutical composition and pharmaceutical preparation for preventing and treating peanut root-knot nematode and preparation method thereof |
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Application publication date: 20120801 |