CN101491260A - Preparation method of female medicine of Mafeng trees - Google Patents
Preparation method of female medicine of Mafeng trees Download PDFInfo
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- CN101491260A CN101491260A CNA2009100941555A CN200910094155A CN101491260A CN 101491260 A CN101491260 A CN 101491260A CN A2009100941555 A CNA2009100941555 A CN A2009100941555A CN 200910094155 A CN200910094155 A CN 200910094155A CN 101491260 A CN101491260 A CN 101491260A
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- mafeng
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- jatropha curcas
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- leaching liquor
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Abstract
The invention belongs to the field of the preparation of botanical insecticides and particularly relates to a method for extracting and preparing a Jatropha curcas extract mother medicine from Jatropha curcas kernels or oil cakes. The method is characterized in that the Jatropha curcas extract mother medicine is prepared by the following steps: the crushed Jatropha curcas kernels or the oil cakes obtained after the Jatropha curcas kernels are pressed oil takes 60 to 95 percent of a protonic solvent as a leaching liquor; the prior solvent or an ultrasonic-assisted mode is adopted to leach, the obtained leaching liquor is merged and spin dried; then the same volume of ethyl acetate and water are added into the leaching liquor and mixed evenly; then unstable components are removed by utilizing acid washing, alkali washing and water scrubbing after a water layer is separated; and the Jatropha curcas extract mother medicine containing 5 to 40 percent of Jatropha curcas extract can be obtained by the degreasing by petroleum ether after an ester layer is spin dried. The method has the advantages of easily obtained raw materials and simple process, and the prepared Jatropha curcas extract mother medicine can be used for preparing pesticide preparations such as insecticides and fungicides.
Description
Technical field
The present invention relates to the plant insecticide technical field, be specifically related to the preparation method of female medicine of Mafeng trees.
Background technology
Active insecticidal components in the botanical pesticide mainly is to separate active components of plants and the secondary metabolites thereof obtain from different parts such as plant roots, stem, leaf and seeds, has that insecticidal spectrum is wide, environmental friendliness, toxicity are low, be difficult for producing resistance and do not injure advantage such as pest natural enemy.Because secondary metabolites is the result that the adaptation of plant self-defense and insect develops coevolution, mode of action variation in addition, as: poisoning, food refusal, avoid keep away, disturb normally grow, photoactivation poisoning etc., so it is can under the condition that is difficult for developing immunity to drugs, act on a plurality of tracts of pest, and safer to non-target organism.
The little seeds of a tung oil tree have very strong drought-enduring, impoverishment tolerant characteristic for Euphorbiaceae Jatropha belongs to half meat dungarunga, fit and are born in xeothermic desert, and extensively plantation helps green barren hill and water and soil conservation in the dry-hot valley area.The kind benevolence oil content of its ripening fruits can reach 40%-60%, and physicochemical property and diesel oil are approaching, can process and refine into biodiesel, has cleaning, efficient, low processing cost, reproducible advantage, receives much concern in epoch of current crude oil scarcity.Multinomial in recent years studies show that, contain terpene, flavonoids, Coumarins, fats, sterols and alkaloids isoreactivity composition in its seed, particularly Jatropha curcas terpene alcohol I wherein, Buddhist ripple ester, Jatropha curcas element etc. have stronger antibacterial, desinsection and anti-molluscan effect, with it as botanical pesticide active ingredient, can research and develop out botanical pesticides such as the new plant insecticide of a class, bactericide, not only, also will open up a new field for the comprehensive utilization of little seeds of a tung oil tree resource for pesticide field provides a kind of new selection.
Summary of the invention
The purpose of this invention is to provide a kind of simple technology and prepare the preparation method of the female medicine of Mafeng trees that can be used for pesticide field.
Technical scheme of the present invention: will put in the suitable digester after the oil cake pulverizing that obtain after little seeds of a tung oil tree kind benevolence or the oil expression of little seeds of a tung oil tree kind benevolence, with the protonic solvents such as ethanol, methyl alcohol and acetone of 60%-95% as leaching liquor, adopt conventional solvent lixiviate or ultrasonic supplementary mode lixiviate, the leaching liquor of gained is merged, is spin-dried for the back add equal-volume ethyl acetate and water, mix, use pickling, alkali cleaning and washing behind the branch vibration layer again, the ester layer is spin-dried for the back and promptly gets the female medicine of Mafeng trees that contains Mafeng trees 5%-40% with petroleum ether degreasing.
The present invention has the following advantages:
The discarded object Jatropha curcas oil cake of little seeds of a tung oil tree kind benevolence after oil expression is fully utilized, and not only can prepare the botanical biological pesticide that can be applicable to agricultural production, also can reduce the biodiesel cost, improves raw material availability.
Embodiment
Embodiment one:
The Jatropha curcas oil cake that 500g pulverizes is put into 2000mL lixiviate still, industrial alcohol with 1: 3 volume ratio adding 95%, add hot reflux, extract 3 times down at 78 ℃, each 60 minutes, the leaching liquor of gained is merged, rotary evaporation adds 500mL ethyl acetate and 500mL water to doing the back, leave standstill after fully mixing, use 200mL 5% hydrochloric acid behind the branch vibration layer more respectively, 200mL5% sodium bicarbonate solution and 200mL water washing, adding 200mL petroleum ether degreasing promptly got and contains female medicine of Mafeng trees after the ester layer was spin-dried for, through efficient liquid phase chromatographic analysis, Mafeng trees content is 10.6%.
Embodiment two:
The Jatropha curcas oil cake that 500g pulverizes is put into 2000mL lixiviate still, ethanolic solution with 1: 3 volume ratio adding 60%, add hot reflux, extract 3 times down at 60 ℃, each 60 minutes, the leaching liquor of gained is merged, rotary evaporation adds 500mL ethyl acetate and 500mL water to doing the back, leave standstill after fully mixing, use 200mL 5% hydrochloric acid behind the branch vibration layer more respectively, 200mL5% sodium bicarbonate solution and 200mL water washing, adding 200mL petroleum ether degreasing promptly got and contains female medicine of Mafeng trees after the ester layer was spin-dried for, through efficient liquid phase chromatographic analysis, Mafeng trees content is 9.6%.
Embodiment three:
The Jatropha curcas oil cake that 500g pulverizes is put into the ultrasonic lixiviate still of 2000mL, ethanolic solution with 1: 3 volume ratio adding 60%, ultrasonic Extraction is 3 times under the room temperature, each 30 minutes, leaching liquor merging, the rotary evaporation of gained are added 500mL ethyl acetate and 500mL water to doing the back, leave standstill after fully mixing, use 200mL 5% hydrochloric acid, 200mL5% sodium bicarbonate solution and 200mL water washing behind the branch vibration layer more respectively, adding 200mL petroleum ether degreasing promptly got and contains female medicine of Mafeng trees after the ester layer was spin-dried for, through efficient liquid phase chromatographic analysis, Mafeng trees content is 9.8%.
Embodiment four:
The Jatropha curcas oil cake that 500g pulverizes is put into 2000mL lixiviate still, industrial methanol with 1: 3 volume ratio adding 95%, add hot reflux, extract 3 times down at 65 ℃, each 60 minutes, the leaching liquor of gained is merged, rotary evaporation adds 500mL ethyl acetate and 500mL water to doing the back, leave standstill after fully mixing, use 200mL 5% hydrochloric acid behind the branch vibration layer more respectively, 200mL5% sodium bicarbonate solution and 200mL water washing, adding 200mL petroleum ether degreasing promptly got and contains female medicine of Mafeng trees after the ester layer was spin-dried for, through efficient liquid phase chromatographic analysis, Mafeng trees content is 12.8%.
Embodiment five:
The little seeds of a tung oil tree kind benevolence that 500g pulverizes is put into 2000mL lixiviate still, methyl alcohol with 1: 3 volume ratio adding 95%, add hot reflux, extract 3 times down at 65 ℃, each 60 minutes, leaching liquor merging, the rotary evaporation of gained are added 500mL ethyl acetate and 500mL water to doing the back, leave standstill after fully mixing, use 200mL 5% hydrochloric acid, 200mL5% sodium bicarbonate solution and 200mL water washing behind the branch vibration layer more respectively, adding 200mL petroleum ether degreasing promptly got and contains female medicine of Mafeng trees after the ester layer was spin-dried for, through efficient liquid phase chromatographic analysis, Mafeng trees content is 6.8%.
Embodiment six:
The little seeds of a tung oil tree kind benevolence that 500g pulverizes is put into the ultrasonic lixiviate still of 2000mL, ethanolic solution with 1: 3 volume ratio adding 60%, ultrasonic Extraction is 3 times under the room temperature, each 30 minutes, leaching liquor merging, the rotary evaporation of gained are added 500mL ethyl acetate and 500mL water to doing the back, leave standstill after fully mixing, use 200mL 5% hydrochloric acid, 200mL5% sodium bicarbonate solution and 200mL water washing behind the branch vibration layer more respectively, adding 200mL petroleum ether degreasing promptly got and contains female medicine of Mafeng trees after the ester layer was spin-dried for, through efficient liquid phase chromatographic analysis, Mafeng trees content is 6.9%.
Claims (5)
1. the preparation method of a female medicine of Mafeng trees, it is characterized in that the oil cake that obtains after the little seeds of a tung oil tree kind benevolence that will pulverize or the oil expression of little seeds of a tung oil tree kind benevolence with the 60%-95% protonic solvent as leaching liquor, adopt conventional solvent lixiviate or ultrasonic supplementary mode lixiviate, the leaching liquor of gained is merged, is spin-dried for the back add equal-volume ethyl acetate and water, mix, behind the branch vibration layer again with pickling, alkali cleaning and washing removing labile element, the ester layer is spin-dried for the back and promptly gets the female medicine of Mafeng trees that contains Mafeng trees 5%-40% with petroleum ether degreasing.
2. the preparation method of a kind of Mafeng trees preparation according to claim 1 is characterized in that little seeds of a tung oil tree kind benevolence or Jatropha curcas oil cake and leaching liquor volume ratio are 1: 3-5, ℃ following extraction is 2-4 time in room temperature～78, each 15 minutes-3 hours.
3. the preparation method of a kind of Mafeng trees preparation according to claim 1 is characterized in that described protonic solvent is ethanol, methyl alcohol and acetone.
4. female medicine of Mafeng trees according to claim 1 is characterized in that with the Mafeng trees being that the insecticidal preparation of main component is 5-40% by weight.
5. according to claim 1,4 described a kind of female medicine of Mafeng trees, it is characterized in that effective active insecticidal components Mafeng trees is made up of the alcohol of the Jatropha curcas terpene in little seeds of a tung oil tree extract I, Buddhist ripple ester, Jatropha curcas element etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100941555A CN101491260A (en) | 2009-03-04 | 2009-03-04 | Preparation method of female medicine of Mafeng trees |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100941555A CN101491260A (en) | 2009-03-04 | 2009-03-04 | Preparation method of female medicine of Mafeng trees |
Publications (1)
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| CN101491260A true CN101491260A (en) | 2009-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2009100941555A Pending CN101491260A (en) | 2009-03-04 | 2009-03-04 | Preparation method of female medicine of Mafeng trees |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897348A (en) * | 2010-05-28 | 2010-12-01 | 云南省农业科学院农业环境资源研究所 | Soil insect control agent and preparation method thereof |
| CN102132708A (en) * | 2011-01-28 | 2011-07-27 | 深圳市东域投资发展有限公司 | Bacteriostat from Jatropha curcas and bacteriostasis application thereof in planting edible mushrooms |
| CN102659583A (en) * | 2012-04-17 | 2012-09-12 | 四川大学 | Method for extraction and separation purification of phorbol ester components of jatropha curcas seeds |
| CN110663706A (en) * | 2019-11-07 | 2020-01-10 | 凉山德农生物能源股份有限公司 | Method for preparing herbicide by using jatropha curcas plant residues |
| CN110692638A (en) * | 2019-10-30 | 2020-01-17 | 凉山德农生物能源股份有限公司 | Wettable sterilizing powder of jatropha curcas extract and preparation method thereof |
| CN110755878A (en) * | 2019-10-30 | 2020-02-07 | 凉山德农生物能源股份有限公司 | Extraction process of jatropha curcas leaf biological crude alkali |
-
2009
- 2009-03-04 CN CNA2009100941555A patent/CN101491260A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897348A (en) * | 2010-05-28 | 2010-12-01 | 云南省农业科学院农业环境资源研究所 | Soil insect control agent and preparation method thereof |
| CN101897348B (en) * | 2010-05-28 | 2013-03-20 | 云南省农业科学院农业环境资源研究所 | Soil insect control agent and preparation method thereof |
| CN102132708A (en) * | 2011-01-28 | 2011-07-27 | 深圳市东域投资发展有限公司 | Bacteriostat from Jatropha curcas and bacteriostasis application thereof in planting edible mushrooms |
| CN102132708B (en) * | 2011-01-28 | 2013-03-27 | 深圳市东域投资发展有限公司 | Bacteriostat from Jatropha curcas and bacteriostasis application thereof in planting edible mushrooms |
| CN102659583A (en) * | 2012-04-17 | 2012-09-12 | 四川大学 | Method for extraction and separation purification of phorbol ester components of jatropha curcas seeds |
| CN110692638A (en) * | 2019-10-30 | 2020-01-17 | 凉山德农生物能源股份有限公司 | Wettable sterilizing powder of jatropha curcas extract and preparation method thereof |
| CN110755878A (en) * | 2019-10-30 | 2020-02-07 | 凉山德农生物能源股份有限公司 | Extraction process of jatropha curcas leaf biological crude alkali |
| CN110663706A (en) * | 2019-11-07 | 2020-01-10 | 凉山德农生物能源股份有限公司 | Method for preparing herbicide by using jatropha curcas plant residues |
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Application publication date: 20090729 |