CN105638783A

CN105638783A - A plant-sourced fungicide for cucumber downy mildew control

Info

Publication number: CN105638783A
Application number: CN201410650397.9A
Authority: CN
Inventors: 张福民
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-11-15
Filing date: 2014-11-15
Publication date: 2016-06-08

Abstract

The invention relates to the technical field of plant disease and pest control, and particularly relates to a plant-sourced fungicide for cucumber downy mildew control. Components having fungicidal functions are extracted from plants and composited into the fungicide that is efficient, nontoxic, plant-sourced and good in downy mildew control effects. According to a technical scheme, the fungicide comprises following raw materials by weight: 5-10 parts of barley seedlings, 2-10 parts of euphorbia pulcherrima, 5-8 parts of sweet-scented oleander, 4-12 parts of ilex, 2-6 parts of eupatorium rugosum, 3-8 parts of golden thread, 6-10 parts of purslane herb, 2-8 parts of clove, 5-9 parts of Japanese thistle herb, 10-12 parts of sweet wormwood herb, 3-9 parts of Chinese pulsatilla root, 1-6 parts of celastrus angulatus, 1-5 parts of ephedra and 1-10 parts of lightyellow sophora root, wherein the barley are seedlings the height of which is 15-20 cm, and the celastrus angulatus relates to celastrus angulatus arils. The fungicide is advantaged by a high efficiency, a low cost, capability of being environmental friendly, difficult generation of drug resistance, no harm to human and livestock, and capability of effectively controlling the downy mildew.

Description

A kind of botanical fungicide preventing and treating cucumber downy mildew

Technical field

The present invention relates to plant pest management technical field, specifically a kind of botanical fungicide preventing and treating cucumber downy mildew.

Background technology

Cucumber downy mildew is the plant disease caused by Mastigomycotina Pseudoperonospora Cuba artificial downy mildew. Being obligate parasite, this disease all can occur from seedling to gathering in the crops each stage, is injured with strain heavier. Mainly cause harm blade, base portion develop to upper leaf. Their early stage forms light yellow subcircular to polygon scab on blade face, it is easy to concurrent angular leaf spot, and when there was dampness in the air, blade back produces the frost mould layer of shape, sometimes can spread to blade face. Later stage scab is withered in flakes, in yellowish-brown, and whole siphonal lobes withered and yellow death time serious, similar verticillium wilt.

Chemical agent has important effect in prevention and elimination of disease and pests, but life-time service makes, most area pest and disease damage fastness frequency 50%, resistance level is up to more than ten thousand times, single pesticide can not controlling disease, and formulation rate is multiplied, bring huge pressure to environment and human health.

Botanical fungicide derives from natural plants because of its effective ingredient, having environmental friendliness in crop disease control, to person poultry safety, disease is not likely to produce the features such as Drug resistance, it is produce the antibacterial kind that non-polluted farm product should preferentially be selected, is therefore also affected by various countries and payes attention to.

Summary of the invention

It is an object of the invention to provide a kind of composition extracting and there is in plant bactericidal action, be combined into a kind of efficiently, antibacterial that non-toxic herb source downy mildew prevention effect is good.

The technical solution adopted in the present invention is: a kind of broad-spectrum bactericide of plant source, it includes following raw material, by weight meter: barley seedlings 5-10 part, poinsettia 2-10 part, Folium seu Cortex Nerii 5-8 part, Ilicis Purpureae 4-12 part, eupatorium ageratoides grass 2-6 part, Rhizoma Coptidis 3-8 part, Herba Portulacae 6-10 part, Flos Caryophylli 2-8 part, Radix Cirsii Japonici 5-9 part, Herba Artemisiae Annuae 10-12 part, Radix Pulsatillae 3-9 part, Celastrus angulatus 1-6 part, Herba Ephedrae 1-5 part, Radix Sophorae Flavescentis 1-10 part.

Preferred: meter by weight: barley seedlings 6-8 part, poinsettia 3-8 part, Folium seu Cortex Nerii 5-8 part, Ilicis Purpureae 6-10 part, eupatorium ageratoides grass 3-5 part, Rhizoma Coptidis 3-6 part, Herba Portulacae 8-10 part, Flos Caryophylli 3-7 part, Radix Cirsii Japonici 6-9 part, Herba Artemisiae Annuae 10-12 part, Radix Pulsatillae 4-8 part, Celastrus angulatus 2-5 part, Herba Ephedrae 2-4 part, Radix Sophorae Flavescentis 4-8 part.

Described Fructus Hordei Vulgaris is highly for the wheat seedling of 15-20cm.

Described Celastrus angulatus is Celastrus angulatus aril.

Preparation method of the present invention: (1) counts the impurity taking raw material removing surface used by weight, stir-fries in cauldron dry or dry in dehydrator, through conventional pulverizer pulverizing, crosses 100 mesh sieves; (2) add ethanol extraction at ambient temperature, add the amount of ethanol to covering mixture surface, after shaking up 5-15 minute, stand and extract 3-5 hour; (3) filter, and under 3000 revs/min of conditions centrifugation 5 minutes, collect centrifugal liquid distill at 70 DEG C, reclaim ethanol, concentrated solution freeze-dried obtains water solublity active ingredient. Filtering gained filtering residue triplication chloroform extraction 4 hours, filter, flush three times with chloroform, merging filtrate, machine centrifugation 5 minutes under 3000 revs/min of conditions, collect centrifugal liquid, (4) obtain the active ingredient arrived, and distillation obtains product.

The saponin existed in barley seedlings can suppress spore germination, the propagation of the pathogenic bacteria of minimizing, and it plays preventive effect effect; From Celastrus angulatus aril, isolation identification goes out 4 kinds of compounds, wherein Compound II per (l ��, 2 ��, 4 ��, 6 ��, 8 ��, 9 ��, 13-seven alkyl-dihydroagarofuran) has bactericidal activity, and research shows to the suppression virulence of peronosclerospora spp. spore germination to be 169.29mg/L; The volatile oil 1-alpha-terpineol that extracts in Herba Ephedrae, ��-terpineol, terpineol-4, myrcene, dihydrocarveol, also all have stronger inhibitory action containing organic acids such as tannin, flavonoid glycoside, saccharide and oxalic acid, citric acid, malic acid, Fumaric acid, benzoic acid, P-hydroxybenzoic acid, cinnamic acid, P-coumaric acid, vanillic acid, protocatechuic acid to phytopathogen etc.; Multiple fungus and antibacterial are had significant inhibitory action by Radix Sophorae Flavescentis ethyl acetate extract, and antibacterial circle diameter is 10��42.nm; Rhizoma Coptidis body rhizome contains substantial amounts of alkaloid: berberine, coptisine, epiberberine, berberrubine, palm leaf coculine, Columbamine, jateorhizine, 13-methyl-.psi.-coptisine., Flos Magnoliae Liliflorae subtract, ferulic acid (, obacunone (obakunone), obakulactone, these alkaloids, the mycelial growth of pathogenic fungi and spore germination have inhibitory action.

The beneficial effects of the present invention is: the present invention produces botanical fungicide after extracting the plant extract compound obtained from pure plant, have efficient, cheap, environmentally friendly, be not likely to produce Drug resistance, to person poultry harmless, the advantage of effectively preventing and treating downy mildew.

Detailed description of the invention

According to present invention, operating instruction in detail below;

Example 1,

Raw materials by weight portion meter: barley seedlings 6 parts, poinsettia 3 parts, Folium seu Cortex Nerii 5 parts, 6 parts of Ilicis Purpureae, eupatorium ageratoides grass 3 parts, Rhizoma Coptidis 3 parts, Herba Portulacae 8 parts, Flos Caryophylli 3 parts, Radix Cirsii Japonici 6 parts, Herba Artemisiae Annuae 10 parts, the Radix Pulsatillae 4 parts, Celastrus angulatus 2 parts, 2 parts of Herba Ephedrae, Radix Sophorae Flavescentis 4 parts.

Described Fructus Hordei Vulgaris is highly for the wheat seedling of 15-20cm.

Described Celastrus angulatus is Celastrus angulatus aril.

Preparation method of the present invention: (1) counts the impurity taking raw material removing surface used by weight, stir-fries in cauldron dry or dry in dehydrator, through conventional pulverizer pulverizing, crosses 100 mesh sieves; (2) add ethanol extraction at ambient temperature, add the amount of ethanol to covering mixture surface, after shaking up 5-15 minute, stand and extract 3-5 hour; (3) filter, and under 3000 revs/min of conditions centrifugation 5 minutes, collect centrifugal liquid distill at 70 DEG C, reclaim ethanol, concentrated solution freeze-dried obtains water solublity active ingredient.Filtering gained filtering residue triplication chloroform extraction 4 hours, filter, flush three times with chloroform, merging filtrate, machine centrifugation 5 minutes under 3000 revs/min of conditions, collect centrifugal liquid, (4) obtain the active ingredient arrived, and distillation obtains product.

Example 2,

Raw materials by weight portion meter: barley seedlings 8 parts, poinsettia 8 parts, Folium seu Cortex Nerii 8 parts, 10 parts of Ilicis Purpureae, eupatorium ageratoides grass 5 parts, Rhizoma Coptidis 6 parts, Herba Portulacae 10 parts, Flos Caryophylli 7 parts, Radix Cirsii Japonici 9 parts, Herba Artemisiae Annuae 12 parts, the Radix Pulsatillae 8 parts, Celastrus angulatus 5 parts, 4 parts of Herba Ephedrae, Radix Sophorae Flavescentis 8 parts.

Described Fructus Hordei Vulgaris is highly for the wheat seedling of 15-20cm.

Described Celastrus angulatus is Celastrus angulatus aril.

Example 3,

Barley seedlings 8 parts, poinsettia 3 parts, Folium seu Cortex Nerii 5 parts, 7 parts of Ilicis Purpureae, eupatorium ageratoides grass 4 parts, Rhizoma Coptidis 5 parts, Herba Portulacae 8 parts, Flos Caryophylli 7 parts, Radix Cirsii Japonici 9 parts, Herba Artemisiae Annuae 11 parts, the Radix Pulsatillae 5 parts, Celastrus angulatus 3 parts, 2 parts of Herba Ephedrae, Radix Sophorae Flavescentis 6 parts.

Preparation method is identical with example 1;

Example 4,

Barley seedlings 7 parts, poinsettia 5 parts, Folium seu Cortex Nerii 6 parts, 9 parts of Ilicis Purpureae, eupatorium ageratoides grass 4 parts, Rhizoma Coptidis 3 parts, Herba Portulacae 8 parts, Flos Caryophylli 4 parts, Radix Cirsii Japonici 8 parts, Herba Artemisiae Annuae 10 parts, the Radix Pulsatillae 5 parts, Celastrus angulatus 5 parts, 3 parts of Herba Ephedrae, Radix Sophorae Flavescentis 7 parts.

Preparation method is identical with example 1.

Example 5,

Barley seedlings 8 parts, poinsettia 8 parts, Folium seu Cortex Nerii 5 parts, 7 parts of Ilicis Purpureae, eupatorium ageratoides grass 5 parts, Rhizoma Coptidis 5 parts, Herba Portulacae 9 parts, Flos Caryophylli 6 parts, Radix Cirsii Japonici 9 parts, Herba Artemisiae Annuae 12 parts, the Radix Pulsatillae 7 parts, Celastrus angulatus 2 parts, 4 parts of Herba Ephedrae, Radix Sophorae Flavescentis 4 parts.

Preparation method is identical with example 1.

Example 6,

Barley seedlings 6 parts, poinsettia 6 parts, Folium seu Cortex Nerii 6 parts, 6 parts of Ilicis Purpureae, eupatorium ageratoides grass 5 parts, Rhizoma Coptidis 5 parts, Herba Portulacae 9 parts, Flos Caryophylli 6 parts, Radix Cirsii Japonici 8 parts, Herba Artemisiae Annuae 11 parts, the Radix Pulsatillae 7 parts, Celastrus angulatus 5 parts, 3 parts of Herba Ephedrae, Radix Sophorae Flavescentis 8 parts.

Preparation method is identical with example 1.

Claims

1. the botanical fungicide preventing and treating cucumber downy mildew, it is characterized in that: a kind of broad-spectrum bactericide of plant source, it includes following raw material, by weight meter: barley seedlings 5-10 part, poinsettia 2-10 part, Folium seu Cortex Nerii 5-8 part, Ilicis Purpureae 4-12 part, eupatorium ageratoides grass 2-6 part, Rhizoma Coptidis 3-8 part, Herba Portulacae 6-10 part, Flos Caryophylli 2-8 part, Radix Cirsii Japonici 5-9 part, Herba Artemisiae Annuae 10-12 part, Radix Pulsatillae 3-9 part, Celastrus angulatus 1-6 part, Herba Ephedrae 1-5 part, Radix Sophorae Flavescentis 1-10 part.

2. the botanical fungicide preventing and treating cucumber downy mildew, it is characterised in that: meter by weight: meter by weight: barley seedlings 6-8 part, poinsettia 3-8 part, Folium seu Cortex Nerii 5-8 part, Ilicis Purpureae 6-10 part, eupatorium ageratoides grass 3-5 part, Rhizoma Coptidis 3-6 part, Herba Portulacae 8-10 part, Flos Caryophylli 3-7 part, Radix Cirsii Japonici 6-9 part, Herba Artemisiae Annuae 10-12 part, Radix Pulsatillae 4-8 part, Celastrus angulatus 2-5 part, Herba Ephedrae 2-4 part, Radix Sophorae Flavescentis 4-8 part.

3. a kind of botanical fungicide preventing and treating cucumber downy mildew according to claim 1, it is characterised in that: described Fructus Hordei Vulgaris is highly wheat seedling for 15-20cm.

4. a kind of botanical fungicide preventing and treating cucumber downy mildew according to claim 1, it is characterised in that: described Celastrus angulatus is Celastrus angulatus aril.