CN107668089A - A kind of botanical fungicide for preventing and treating cucumber downy mildew and preparation method thereof - Google Patents
A kind of botanical fungicide for preventing and treating cucumber downy mildew and preparation method thereof Download PDFInfo
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- CN107668089A CN107668089A CN201710897963.XA CN201710897963A CN107668089A CN 107668089 A CN107668089 A CN 107668089A CN 201710897963 A CN201710897963 A CN 201710897963A CN 107668089 A CN107668089 A CN 107668089A
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- botanical fungicide
- downy mildew
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- Agronomy & Crop Science (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The invention discloses a kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:10 30 parts of artemisia annua, 15 25 parts of alfalfa, 8 15 parts of all sorts of flowers daghestan sweet clover, 8 15 parts of adnate elder herb, 5 10 parts of Tangut Anisodus Radix, 5 10 parts of granatum, 5 20 parts of emulsifying agent, 20 40 parts of cosolvent, 15 25 parts of bleeding agent, 40 70 parts of solvent.What the botanical fungicide of the present invention developed specifically for cucumber downy mildew, with strong points, bactericidal effect is good, and noresidue is pollution-free, the edible cucumber that people can trust.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of botanical fungicide and its system for preventing and treating cucumber downy mildew
Preparation Method.
Background technology
Cucumber downy mildew is caused by Oomycete mould category fungal attack, is occurred more after yielding positive results, from bottom old leaf
Start to fall ill.There is water stain shape, light green color spot in early stage, vacuum side of blade, and it is in polygonal to be limited after expansion by vein, scab
Color change is green, yellow, eventually becomes brown, the mould layer of atropurpureus is grown on the scab of the moisture conditions lower blade back side.Cucumber
Downy mildew is a kind of destructive disease, is commonly called as " horse race is dry ", " dry leaves ", once morbidity, development is swift and violent, so sending out ahead of time
Existing, preventing and treating is very crucial in time.This disease can all occur in each breeding time, mainly cause harm blade relatively conventional with the middle and late growth stage,
Often elder generation is at canopy room droplet dew or the place that prevents wind from passing through, formation infection center are rapid afterwards to spread.
The appearance and use of chemical pesticide, while crop yield and agricultural productive force is greatly improved, unavoidably
Bring some negative effects on ground, such as the harm to human health, the pollution to environment, the influence to non-target organism, resistance
Development and agriculture stability decrease etc..With the enhancing of people's environmental consciousness, highly desirable to find mechanism of action only
Special and environment amenable new control agent, in this case, plant-source antibacterial material is because good, right with being compatible with the environment property
The selection pressure of harmful organism is small, meets the requirement of social sustainable development, and has attracted the pass of more and more pesticide research persons
Note, turn into bactericide research and development focus in the world.
The content of the invention
For above-mentioned, it is an object of the invention to provide a kind of botanical fungicide for preventing and treating cucumber downy mildew and its preparation side
Method.
The present invention adopts the technical scheme that:
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua
10-30 parts, alfalfa 15-25 parts, all sorts of flowers daghestan sweet clover 8-15 parts, adnate elder herb 8-15 parts, Tangut Anisodus Radix 5-10 parts, granatum 5-10
Part, emulsifying agent 5-20 parts, cosolvent 20-40 parts, bleeding agent 15-25 parts, solvent 40-70 parts.
Preferably, the botanical fungicide of described preventing and treating cucumber downy mildew, in parts by mass, including following components are former
Material:20 parts of artemisia annua, 20 parts of alfalfa, 11.5 parts of all sorts of flowers daghestan sweet clover, 11.5 parts of adnate elder herb, 7.5 parts of Tangut Anisodus Radix, granatum
7.5 parts, 12.5 parts of emulsifying agent, 30 parts of cosolvent, 20 parts of bleeding agent, 55 parts of solvent.
Further, described emulsifying agent is Tween-80.
Further, described cosolvent is absolute ethyl alcohol.
Further, described bleeding agent is azone.
Further, described solvent is distilled water.
Present invention also offers the preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, including following step
Suddenly:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed,
Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, 20-24 hours is soaked at 25 DEG C, filters to get filtrate, repeats to soak
3 times, 3 filtrates are mixed, wherein, methanol volume fraction be 50-70%, and the mass ratio of plant mixed powder and methanol is 1:8-
10;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant
Material resource bactericide.
It is an advantage of the invention that:What the botanical fungicide of the present invention developed specifically for cucumber downy mildew, for
Property is strong, and bactericidal effect is good, noresidue, pollution-free, the edible cucumber that people can trust.
Embodiment
Presently preferred embodiments of the present invention is described in detail below so that advantages and features of the invention can be easier to by
It will be appreciated by those skilled in the art that apparent clearly defined so as to be made to protection scope of the present invention.
Embodiment 1
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua 10
Part, 15 parts of alfalfa, 8 parts of all sorts of flowers daghestan sweet clover, 8 parts of adnate elder herb, 5 parts of Tangut Anisodus Radix, 5 parts of granatum, 5 parts of emulsifying agent, cosolvent
20 parts, 15 parts of bleeding agent, 40 parts of solvent.
Wherein, described emulsifying agent is Tween-80;Described cosolvent is absolute ethyl alcohol;Described bleeding agent is bay
Azone;Described solvent is distilled water.
The preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, comprises the following steps:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed,
Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, soaks 20 hours, filters to get filtrate at 25 DEG C, repeat immersion 3
It is secondary, 3 filtrates are mixed, wherein, methanol volume fraction is 50%, and the mass ratio of plant mixed powder and methanol is 1:8;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant
Material resource bactericide.
Embodiment 2
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua 20
Part, 20 parts of alfalfa, 11.5 parts of all sorts of flowers daghestan sweet clover, 11.5 parts of adnate elder herb, 7.5 parts of Tangut Anisodus Radix, 7.5 parts of granatum, emulsifying agent
12.5 parts, 30 parts of cosolvent, 20 parts of bleeding agent, 55 parts of solvent.
Wherein, described emulsifying agent is Tween-80;Described cosolvent is absolute ethyl alcohol;Described bleeding agent is bay
Azone;Described solvent is distilled water.
The preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, comprises the following steps:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed,
Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, soaks 22 hours, filters to get filtrate at 25 DEG C, repeat immersion 3
It is secondary, 3 filtrates are mixed, wherein, methanol volume fraction is 60%, and the mass ratio of plant mixed powder and methanol is 1:9;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant
Material resource bactericide.
Embodiment 3
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua 30
Part, 25 parts of alfalfa, 15 parts of all sorts of flowers daghestan sweet clover, 15 parts of adnate elder herb, 10 parts of Tangut Anisodus Radix, 10 parts of granatum, 20 parts of emulsifying agent, help
40 parts of solvent, 25 parts of bleeding agent, 70 parts of solvent.
Wherein, described emulsifying agent is Tween-80;Described cosolvent is absolute ethyl alcohol;Described bleeding agent is bay
Azone;Described solvent is distilled water.
The preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, comprises the following steps:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed,
Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, soaks 24 hours, filters to get filtrate at 25 DEG C, repeat immersion 3
It is secondary, 3 filtrates are mixed, wherein, methanol volume fraction is 70%, and the mass ratio of plant mixed powder and methanol is 1:10;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant
Material resource bactericide.
Through experiment, the botanical fungicide of the preventing and treating cucumber downy mildew prepared by 1-3 of the embodiment of the present invention is diluted 100 times
Afterwards, to the bacteriostasis rate of bacterium of downy mildew of cucumber, 95.7%, 97.2%, 96.3% has been respectively reached, it can be seen that, plant of the invention
Material resource bactericide has good prevention effect to cucumber downy mildew.
Claims (7)
- A kind of 1. botanical fungicide for preventing and treating cucumber downy mildew, it is characterised in that in parts by mass, including following component raw materials: Artemisia annua 10-30 parts, alfalfa 15-25 parts, all sorts of flowers daghestan sweet clover 8-15 parts, adnate elder herb 8-15 parts, Tangut Anisodus Radix 5-10 parts, pomegranate Skin 5-10 parts, emulsifying agent 5-20 parts, cosolvent 20-40 parts, bleeding agent 15-25 parts, solvent 40-70 parts.
- 2. botanical fungicide according to claim 1, it is characterised in that in parts by mass, including following component raw materials: 20 parts of artemisia annua, 20 parts of alfalfa, 11.5 parts of all sorts of flowers daghestan sweet clover, 11.5 parts of adnate elder herb, 7.5 parts of Tangut Anisodus Radix, granatum 7.5 Part, 12.5 parts of emulsifying agent, 30 parts of cosolvent, 20 parts of bleeding agent, 55 parts of solvent.
- 3. botanical fungicide according to claim 1 or 2, it is characterised in that described emulsifying agent is Tween-80.
- 4. botanical fungicide according to claim 1 or 2, it is characterised in that described cosolvent is absolute ethyl alcohol.
- 5. botanical fungicide according to claim 1 or 2, it is characterised in that described bleeding agent is azone.
- 6. botanical fungicide according to claim 1 or 2, it is characterised in that described solvent is distilled water.
- 7. the preparation method of the botanical fungicide of preventing and treating cucumber downy mildew according to claim 1 or 2, it is characterised in that Comprise the following steps:(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, is mixed, and are crushed, Obtain plant mixed powder;(2) methanol immersion is poured into plant mixed powder, 20-24 hours is soaked at 25 DEG C, filters to get filtrate, repeat immersion 3 times, 3 filtrates are mixed, wherein, methanol volume fraction be 50-70%, and the mass ratio of plant mixed powder and methanol is 1:8-10;(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, obtain plant source Bactericide.
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Citations (6)
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CN1181187A (en) * | 1997-11-20 | 1998-05-13 | 贾春健 | Agricultural traditional Chinese medicine bactericide |
CN102578170A (en) * | 2012-01-11 | 2012-07-18 | 蔡葵荣 | Chinese herbal medicinal bactericide |
CN103828861A (en) * | 2012-11-23 | 2014-06-04 | 青岛艾华隆生物科技有限公司 | Aqueous suspoemulsified green biological pesticide |
CN105123811A (en) * | 2015-08-31 | 2015-12-09 | 王璐 | Environment-friendly microbial pesticide utilizing biological resources |
CN105638783A (en) * | 2014-11-15 | 2016-06-08 | 张福民 | A plant-sourced fungicide for cucumber downy mildew control |
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2017
- 2017-09-28 CN CN201710897963.XA patent/CN107668089A/en active Pending
Patent Citations (6)
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CN1181187A (en) * | 1997-11-20 | 1998-05-13 | 贾春健 | Agricultural traditional Chinese medicine bactericide |
CN102578170A (en) * | 2012-01-11 | 2012-07-18 | 蔡葵荣 | Chinese herbal medicinal bactericide |
CN103828861A (en) * | 2012-11-23 | 2014-06-04 | 青岛艾华隆生物科技有限公司 | Aqueous suspoemulsified green biological pesticide |
CN105638783A (en) * | 2014-11-15 | 2016-06-08 | 张福民 | A plant-sourced fungicide for cucumber downy mildew control |
CN105123811A (en) * | 2015-08-31 | 2015-12-09 | 王璐 | Environment-friendly microbial pesticide utilizing biological resources |
CN107079941A (en) * | 2017-06-08 | 2017-08-22 | 合肥龙滨化工科技有限公司 | A kind of botanical pesticide for preventing and treating muskmelon downy mildew |
Non-Patent Citations (4)
Title |
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叶萱等: "紫花苜蓿中的三萜皂苷可作抗稻瘟病菌的真菌剂", 《世界农药》 * |
吴静等: "黄花蒿(Artemisia annua L.)提取物对两种病原真菌的生物活性", 《农药》 * |
四川省农业科学院农药研究室: "《农药分析》", 31 August 1976, 石油化学工业出版社 * |
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