CN107668089A - A kind of botanical fungicide for preventing and treating cucumber downy mildew and preparation method thereof - Google Patents

A kind of botanical fungicide for preventing and treating cucumber downy mildew and preparation method thereof Download PDF

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Publication number
CN107668089A
CN107668089A CN201710897963.XA CN201710897963A CN107668089A CN 107668089 A CN107668089 A CN 107668089A CN 201710897963 A CN201710897963 A CN 201710897963A CN 107668089 A CN107668089 A CN 107668089A
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Prior art keywords
parts
botanical fungicide
downy mildew
cosolvent
solvent
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CN201710897963.XA
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Inventor
韦汀怡
邹志平
李丽花
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Liuzhou Yarui Technology Co Ltd
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Liuzhou Yarui Technology Co Ltd
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Priority to CN201710897963.XA priority Critical patent/CN107668089A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/38Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:10 30 parts of artemisia annua, 15 25 parts of alfalfa, 8 15 parts of all sorts of flowers daghestan sweet clover, 8 15 parts of adnate elder herb, 5 10 parts of Tangut Anisodus Radix, 5 10 parts of granatum, 5 20 parts of emulsifying agent, 20 40 parts of cosolvent, 15 25 parts of bleeding agent, 40 70 parts of solvent.What the botanical fungicide of the present invention developed specifically for cucumber downy mildew, with strong points, bactericidal effect is good, and noresidue is pollution-free, the edible cucumber that people can trust.

Description

A kind of botanical fungicide for preventing and treating cucumber downy mildew and preparation method thereof
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of botanical fungicide and its system for preventing and treating cucumber downy mildew Preparation Method.
Background technology
Cucumber downy mildew is caused by Oomycete mould category fungal attack, is occurred more after yielding positive results, from bottom old leaf Start to fall ill.There is water stain shape, light green color spot in early stage, vacuum side of blade, and it is in polygonal to be limited after expansion by vein, scab Color change is green, yellow, eventually becomes brown, the mould layer of atropurpureus is grown on the scab of the moisture conditions lower blade back side.Cucumber Downy mildew is a kind of destructive disease, is commonly called as " horse race is dry ", " dry leaves ", once morbidity, development is swift and violent, so sending out ahead of time Existing, preventing and treating is very crucial in time.This disease can all occur in each breeding time, mainly cause harm blade relatively conventional with the middle and late growth stage, Often elder generation is at canopy room droplet dew or the place that prevents wind from passing through, formation infection center are rapid afterwards to spread.
The appearance and use of chemical pesticide, while crop yield and agricultural productive force is greatly improved, unavoidably Bring some negative effects on ground, such as the harm to human health, the pollution to environment, the influence to non-target organism, resistance Development and agriculture stability decrease etc..With the enhancing of people's environmental consciousness, highly desirable to find mechanism of action only Special and environment amenable new control agent, in this case, plant-source antibacterial material is because good, right with being compatible with the environment property The selection pressure of harmful organism is small, meets the requirement of social sustainable development, and has attracted the pass of more and more pesticide research persons Note, turn into bactericide research and development focus in the world.
The content of the invention
For above-mentioned, it is an object of the invention to provide a kind of botanical fungicide for preventing and treating cucumber downy mildew and its preparation side Method.
The present invention adopts the technical scheme that:
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua 10-30 parts, alfalfa 15-25 parts, all sorts of flowers daghestan sweet clover 8-15 parts, adnate elder herb 8-15 parts, Tangut Anisodus Radix 5-10 parts, granatum 5-10 Part, emulsifying agent 5-20 parts, cosolvent 20-40 parts, bleeding agent 15-25 parts, solvent 40-70 parts.
Preferably, the botanical fungicide of described preventing and treating cucumber downy mildew, in parts by mass, including following components are former Material:20 parts of artemisia annua, 20 parts of alfalfa, 11.5 parts of all sorts of flowers daghestan sweet clover, 11.5 parts of adnate elder herb, 7.5 parts of Tangut Anisodus Radix, granatum 7.5 parts, 12.5 parts of emulsifying agent, 30 parts of cosolvent, 20 parts of bleeding agent, 55 parts of solvent.
Further, described emulsifying agent is Tween-80.
Further, described cosolvent is absolute ethyl alcohol.
Further, described bleeding agent is azone.
Further, described solvent is distilled water.
Present invention also offers the preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, including following step Suddenly:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed, Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, 20-24 hours is soaked at 25 DEG C, filters to get filtrate, repeats to soak 3 times, 3 filtrates are mixed, wherein, methanol volume fraction be 50-70%, and the mass ratio of plant mixed powder and methanol is 1:8- 10;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant Material resource bactericide.
It is an advantage of the invention that:What the botanical fungicide of the present invention developed specifically for cucumber downy mildew, for Property is strong, and bactericidal effect is good, noresidue, pollution-free, the edible cucumber that people can trust.
Embodiment
Presently preferred embodiments of the present invention is described in detail below so that advantages and features of the invention can be easier to by It will be appreciated by those skilled in the art that apparent clearly defined so as to be made to protection scope of the present invention.
Embodiment 1
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua 10 Part, 15 parts of alfalfa, 8 parts of all sorts of flowers daghestan sweet clover, 8 parts of adnate elder herb, 5 parts of Tangut Anisodus Radix, 5 parts of granatum, 5 parts of emulsifying agent, cosolvent 20 parts, 15 parts of bleeding agent, 40 parts of solvent.
Wherein, described emulsifying agent is Tween-80;Described cosolvent is absolute ethyl alcohol;Described bleeding agent is bay Azone;Described solvent is distilled water.
The preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, comprises the following steps:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed, Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, soaks 20 hours, filters to get filtrate at 25 DEG C, repeat immersion 3 It is secondary, 3 filtrates are mixed, wherein, methanol volume fraction is 50%, and the mass ratio of plant mixed powder and methanol is 1:8;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant Material resource bactericide.
Embodiment 2
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua 20 Part, 20 parts of alfalfa, 11.5 parts of all sorts of flowers daghestan sweet clover, 11.5 parts of adnate elder herb, 7.5 parts of Tangut Anisodus Radix, 7.5 parts of granatum, emulsifying agent 12.5 parts, 30 parts of cosolvent, 20 parts of bleeding agent, 55 parts of solvent.
Wherein, described emulsifying agent is Tween-80;Described cosolvent is absolute ethyl alcohol;Described bleeding agent is bay Azone;Described solvent is distilled water.
The preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, comprises the following steps:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed, Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, soaks 22 hours, filters to get filtrate at 25 DEG C, repeat immersion 3 It is secondary, 3 filtrates are mixed, wherein, methanol volume fraction is 60%, and the mass ratio of plant mixed powder and methanol is 1:9;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant Material resource bactericide.
Embodiment 3
A kind of botanical fungicide for preventing and treating cucumber downy mildew, in parts by mass, including following component raw materials:Artemisia annua 30 Part, 25 parts of alfalfa, 15 parts of all sorts of flowers daghestan sweet clover, 15 parts of adnate elder herb, 10 parts of Tangut Anisodus Radix, 10 parts of granatum, 20 parts of emulsifying agent, help 40 parts of solvent, 25 parts of bleeding agent, 70 parts of solvent.
Wherein, described emulsifying agent is Tween-80;Described cosolvent is absolute ethyl alcohol;Described bleeding agent is bay Azone;Described solvent is distilled water.
The preparation method of the botanical fungicide of above-mentioned preventing and treating cucumber downy mildew, comprises the following steps:
(1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, are mixed, Crush, obtain plant mixed powder;
(2) methanol immersion is poured into plant mixed powder, soaks 24 hours, filters to get filtrate at 25 DEG C, repeat immersion 3 It is secondary, 3 filtrates are mixed, wherein, methanol volume fraction is 70%, and the mass ratio of plant mixed powder and methanol is 1:10;
(3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
(4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, must plant Material resource bactericide.
Through experiment, the botanical fungicide of the preventing and treating cucumber downy mildew prepared by 1-3 of the embodiment of the present invention is diluted 100 times Afterwards, to the bacteriostasis rate of bacterium of downy mildew of cucumber, 95.7%, 97.2%, 96.3% has been respectively reached, it can be seen that, plant of the invention Material resource bactericide has good prevention effect to cucumber downy mildew.

Claims (7)

  1. A kind of 1. botanical fungicide for preventing and treating cucumber downy mildew, it is characterised in that in parts by mass, including following component raw materials: Artemisia annua 10-30 parts, alfalfa 15-25 parts, all sorts of flowers daghestan sweet clover 8-15 parts, adnate elder herb 8-15 parts, Tangut Anisodus Radix 5-10 parts, pomegranate Skin 5-10 parts, emulsifying agent 5-20 parts, cosolvent 20-40 parts, bleeding agent 15-25 parts, solvent 40-70 parts.
  2. 2. botanical fungicide according to claim 1, it is characterised in that in parts by mass, including following component raw materials: 20 parts of artemisia annua, 20 parts of alfalfa, 11.5 parts of all sorts of flowers daghestan sweet clover, 11.5 parts of adnate elder herb, 7.5 parts of Tangut Anisodus Radix, granatum 7.5 Part, 12.5 parts of emulsifying agent, 30 parts of cosolvent, 20 parts of bleeding agent, 55 parts of solvent.
  3. 3. botanical fungicide according to claim 1 or 2, it is characterised in that described emulsifying agent is Tween-80.
  4. 4. botanical fungicide according to claim 1 or 2, it is characterised in that described cosolvent is absolute ethyl alcohol.
  5. 5. botanical fungicide according to claim 1 or 2, it is characterised in that described bleeding agent is azone.
  6. 6. botanical fungicide according to claim 1 or 2, it is characterised in that described solvent is distilled water.
  7. 7. the preparation method of the botanical fungicide of preventing and treating cucumber downy mildew according to claim 1 or 2, it is characterised in that Comprise the following steps:
    (1) artemisia annua, alfalfa, all sorts of flowers daghestan sweet clover, adnate elder herb, Tangut Anisodus Radix, granatum are weighed in mass ratio, is mixed, and are crushed, Obtain plant mixed powder;
    (2) methanol immersion is poured into plant mixed powder, 20-24 hours is soaked at 25 DEG C, filters to get filtrate, repeat immersion 3 times, 3 filtrates are mixed, wherein, methanol volume fraction be 50-70%, and the mass ratio of plant mixed powder and methanol is 1:8-10;
    (3) filtrate for preparing step (2), is evaporated to medicinal extract with Rotary Evaporators, obtains plant source medicinal extract;
    (4) medicinal extract prepared by step (3) is mixed with emulsifying agent, cosolvent, bleeding agent part, solvent, stirs, obtain plant source Bactericide.
CN201710897963.XA 2017-09-28 2017-09-28 A kind of botanical fungicide for preventing and treating cucumber downy mildew and preparation method thereof Pending CN107668089A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1181187A (en) * 1997-11-20 1998-05-13 贾春健 Agricultural traditional Chinese medicine bactericide
CN102578170A (en) * 2012-01-11 2012-07-18 蔡葵荣 Chinese herbal medicinal bactericide
CN103828861A (en) * 2012-11-23 2014-06-04 青岛艾华隆生物科技有限公司 Aqueous suspoemulsified green biological pesticide
CN105123811A (en) * 2015-08-31 2015-12-09 王璐 Environment-friendly microbial pesticide utilizing biological resources
CN105638783A (en) * 2014-11-15 2016-06-08 张福民 A plant-sourced fungicide for cucumber downy mildew control
CN107079941A (en) * 2017-06-08 2017-08-22 合肥龙滨化工科技有限公司 A kind of botanical pesticide for preventing and treating muskmelon downy mildew

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1181187A (en) * 1997-11-20 1998-05-13 贾春健 Agricultural traditional Chinese medicine bactericide
CN102578170A (en) * 2012-01-11 2012-07-18 蔡葵荣 Chinese herbal medicinal bactericide
CN103828861A (en) * 2012-11-23 2014-06-04 青岛艾华隆生物科技有限公司 Aqueous suspoemulsified green biological pesticide
CN105638783A (en) * 2014-11-15 2016-06-08 张福民 A plant-sourced fungicide for cucumber downy mildew control
CN105123811A (en) * 2015-08-31 2015-12-09 王璐 Environment-friendly microbial pesticide utilizing biological resources
CN107079941A (en) * 2017-06-08 2017-08-22 合肥龙滨化工科技有限公司 A kind of botanical pesticide for preventing and treating muskmelon downy mildew

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
叶萱等: "紫花苜蓿中的三萜皂苷可作抗稻瘟病菌的真菌剂", 《世界农药》 *
吴静等: "黄花蒿(Artemisia annua L.)提取物对两种病原真菌的生物活性", 《农药》 *
四川省农业科学院农药研究室: "《农药分析》", 31 August 1976, 石油化学工业出版社 *
苏生等: "豆科植物杀虫杀菌资源及其活性成分研究进展", 《现代农业科技》 *

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