KR101072731B1 - Fermentation composition using verniciflua the manufacturing method - Google Patents

Abstract

The present invention relates to a method for producing a sesame and lacquer fermentation composition that can be effectively used in the body by detoxifying volatile urushiol substances causing skin allergies using lacquer and lacquer.
That is, the present invention is soaked by dipping distilled water of the sumac and sesame bark in distilled water, soaked enough to spread the rice straw and chestnut leaves and spread the sesame and lacquer bark evenly on top of it again and then repeatedly the rice straw and chestnut leaves, sesame Lacquer and lacquer layers are first fermented to reduce the volatile urushiol substances that cause skin allergies contained in oak and bark to facilitate work, and are nontoxic to dry residual urushiol. It is characterized by a method of producing fermentation compositions using lacquer and lacquer bark, which are prepared by mixing several medicinal herbs in the pre-fermentation step and crushing them, followed by secondary fermentation and tertiary fermentation.

Description

Fermentation composition using lacquer and lacquer bark {Fermentation composition using Verniciflua the manufacturing method}

The present invention relates to a method for producing a sesame and lacquer fermentation composition that can be effectively used in the body by detoxifying volatile urushiol substances causing skin allergies using lacquer and lacquer.

In general, lacquer has a warm nature and sour taste, poisonous kills the insects, eliminates blood, and dysmenorrhea, sangu and red meat.

Dried lacquer is called dried in oriental medicine, and in the Joseon Dynasty, Uiseong Heo-jun said, in addition to releasing fish and blood and women's blemishes, in the Dongyebogam, 'Deep lacquer is used to kill the parasite and kill the parasite. To rule over. ' In addition, Hwang Do Yeon writes about the effect of lacquer in the medicine plywood.

 Fish blood is bruises in the flesh and blood lumps together. Sangu refers to acidosis in which the lower abdomen is swollen and hurts and urine does not go well. Catching is eaten and pretended to build up in the stomach for a long time. Substances that help release blood and catches and help circulation of blood and body fluids generally help treat and cure chronic diseases.

 The toxicity of lacquer is that it kills insects such as parasites, as well as bacteria and bacteria. There are many publications on the efficacy of lacquer in recent studies, but among them, the research has attracted attention as it has been shown to have excellent anti-cancer effects. In addition, when the wife has a cup-size solid mass in the lower abdomen, menstruation is irregular, sometimes seizures occur, dysentery occurs and day by day, even when the treatment with lacquer-containing medicine is said to improve symptoms.

If you have a pain in the lower back due to blood and bruises or bruises that hurt the muscles or skeleton, eating the lacquer removes the blood and strengthens the muscles and skeleton.

Even if you live in a cold place for a long time or stay in a cold place for a long time due to sudden changes in your environment, if you have pain in your arms and legs, the pain will subside and your numbness will go away.

For modern scientific research, the research team of the Korea Institute of Science and Technology (KIST) Lac researchers found urushiol in the sap of Korean domestic lacquer in 1997, and tested it at a concentration of 3-4PPM to detect cancer cells in human cancer cell lines. He reported that he had excellent anti-cancer activity, and another study found that urushiol contained in Wonju lacquer in Gangwon-do was 50% higher than other fats.

In addition, the Forest Service Forestry Research Institute's Lac Research Team published the same results at a similar time. The contents were analyzed by urushiol in lacquer sap to extract MU2 ingredient with excellent anticancer effect. MU2 extracted from Hwachil obtained by heat treatment of lacquer tree is not only excellent anticancer effect but also irrelevant to allergic phenomenon of lacquer. It is a safe compound. The substance is said to be much more effective in inhibiting the growth of animal blood cancer cells, human lung cancer cells and stomach cancer cells than the existing anticancer drug tetraplatin. Much less than an anticancer agent has the same effect, converts cancer cells into normal cells and prevents the rapid proliferation of cancer cells that may appear after tumor incision surgery. The team also said MU2 has antioxidant and hangover-relieving properties.

Also, lacquer is known to kill various microorganisms such as bacteria and bacteria and to prevent insects. Since the toxicity of lacquer completely prevents microorganisms and insects, it has been used as a paint to protect fine woodwork and furniture. It is used as a paint for protection.

The effect of killing various germs and microorganisms such as bacteria and preventing insects also works in the human body to kill parasites and prevent various bacteria to remove inflammation. If children are hungry due to roundworms, the lacquer tree is split into fires, toxic to smoke, and charcoal powder is fed on an empty stomach.

There are two types of lacquer: short lacquer and tall lacquer. Among them, sesame seeds are highly toxic and strong. The essence, bark, and firewood are processed and used according to their intended use.

It is known that chestnut leaves are effective for allergic skin diseases caused by lacquer in private prescription, and it is said that it is good to take a bath with boiled water.If you put chestnut leaves in a bag and soak them in boiling water for a long time This is known to improve. A simpler method is to crush the raw leaves and apply the juice.

Even people who have the usual constitution that lacquer rises well will not suffer if the lacquer rises if you take the water with the decoction of chestnut leaves.

In addition, research to detoxify urushiol, an allergen contained in modern scientific lacquer, has been studied by many researchers one after another, and papers have been published in the society.

Rice straw is a treasure trove of soil microorganisms, and it contains not only the microorganisms necessary for making meju or Cheonggukjang, but also many microorganisms that are beneficial to our human beings, and penicillium, a microorganism that makes penicillin. It can neutralize the strong antimicrobial substances of urushiol. In addition, the composition and use of herbal medicine (Science Encyclopedia Publisher-Kyung Hee University College of Medicine) recommends that chestnut leaves are effective against dermatitis caused by lacquer, reducing the toxicity of allergic volatile urushiol through primary fermentation. Because of the giving effect was used mixed with rice straw. In addition, it is believed that functional substances of secondary metabolites can be obtained by fermenting and mixing several microorganisms of Bacillus subtilis and Rare Actinomycetes in the second stage.

On the other hand, the microorganisms used in the fermentation is an important value in the biomaterials industry because of its high added value is recognized as useful. Many countries around the world are using microorganisms to search for new substances that can be used in various ways, such as antibiotics, anticancer drugs, pesticides, insecticides, herbicides, pesticides and health supplements, and food materials such as enzymes. To date, more than 10,000 new substances have been discovered from microorganisms, and about 100 new substances have already been put into practical use.

Among the microorganisms, Bacillus subtilis to be used in the present invention is an aerobic by-product bacillus and is a very small microorganism of less than 1 μm in diameter. Sugars are the carbon source, and ammonium salts are the nitrogen source when making antibiotics. The fungus is not used to cure illnesses, but rather as a fungicide for odor or canning. Actinomycetes are also recognized as the most important microorganisms in the industry due to the diversity of chemical structures and species abundance, especially in secondary metabolites. About two-thirds of the 10,000 bioactive substances found so far from actinomycetes Derived. Actinomycetes are expected to occupy an important position in the biomaterials industry through the isolation of new strains and gene function.

On the other hand, actinomycetes have been classified as fungi in the past because they form fungus-like forms, but as the physiological and biochemical analysis methods have been developed since the 1970s, they are recognized as protozoa with high GC content and differentiation properties. Prior to the 1960's, Streptomyces sp.

Later, when gentamycin was discovered from actinomycetes of Micromonospora, rare actinomycetes other than the genus Streptomyces began to receive much attention. In 1989 Bergey's Manual of Systematic Bacteriology (1) describes 40 species of rare actinomycetes.

In order to convert lacquer and lacquer into fermentation compositions useful for the human body, firstly, distilled water diluted with miso rich in microorganisms is immersed in the distilled water and the substance of crushed and sliced oak and sesame shells to infiltrate microorganisms and then straw and chestnut leaves. It is mixed with fermentation and adds several herbal medicines that exhibit the second functionality and fermentation microorganisms are administered through the second and third fermentation process to detoxify sumac and lacquer bark to provide useful fermentation composition to human body.

In the present invention, in preparing a fermentation composition using the lacquer and lacquer bark, dilute the sumac and sesame bark in distilled water to be soaked, soaked enough to spread the straw and chestnut leaves and evenly spread the lacquer and lacquer on it. After repeating the layering of rice straw, chestnut leaves, oak and lacquer layers, the primary fermentation is carried out to reduce the volatile urushiol substances that cause skin allergy contained in the oak and bark to facilitate the work. Fermentation composition using lacquer and lacquer bark prepared by secondary and tertiary fermentation process by mixing several herbal medicines at the stage of fermentation to detoxify the residual urushiol and detoxification Characterized in the manufacturing method.

Fermentation composition using the lacquer and lacquer bark according to the present invention can remove the toxicity of the lacquer and lacquer bark and the active ingredient of the lacquer and lacquer bark can be absorbed in the body as much as possible and provides a useful fermentation product to the human body There is.

Figure 1 shows a fermentation composition manufacturing step using the lacquer and lacquer bark according to the present invention.

In the present invention, soaking the oak and sesame bark in water diluted with miso is enough to infiltrate the rare soil microorganisms contained in the miso into the oak and oak bark, and a layer is stacked on the straw and chestnut leaves to ferment. The process of making rice straw is the habitat of soil microorganisms, so that more microorganisms infiltrate the oak and the bark of the bark to help fermentation, and the chestnut leaves reduce the toxicity of the volatile urushiol contained in the bark and the bark of the bark. In order to perform the 2nd and 3rd work, to avoid the skin allergy of the worker, the mixing of various herbal medicines is to make it possible to use variously for various symptoms, and the 2nd and 3rd fermentation process with Bacillus subtilis and rare actinomycetes. Going through fermentation can detoxify volatile urushiol substances that cause skin allergies. Consists of, poison ivy and poison oak bark to be used in the present invention is indeed preferred to use the sumac and poison oak bark true.

This will be described in more detail as follows.

1) Shred and slice lacquer and lacquer, dilute soybean paste in distilled water by 3-30% by weight, soak for 1 to 10 hours and soak it, and then make lacquer and lacquer medium to remove moisture for 1-2 hours. Step S10;

In the above step, lacquer and lacquer are crushed or sliced, and soybean paste is soaked in distilled water diluted 3-30% by weight and soaked sufficiently for 1 to 10 hours. Once the microorganisms and nutrients are infiltrated enough, drain them out of the sieve for 1 to 2 hours to drain them, and dry the lacquer and lacquer bark on the skin of the crushed or sliced material.

2) Covering the rice straw and chestnut leaves 1 ~ 20 ㎝ After spreading the oak and the lacquer bark 5 ~ 20 ㎝ and repeatedly stacked the straw and chestnut leaves 1 ~ 20 ㎝ by fermentation at 15 ~ 50 ℃ (S20) ;

The rice straw and chestnut leaves evenly spread the 1 ~ 20 cm provided in the above to provide the effect of reducing the toxicity of urushiol, which is an allergic substance, to the oak and the lacquer shell shredded or sliced medium. Should be provided.

3) 100 parts by weight of fermentation products produced in the first fermentation step and white berry, halved, Angelica, licorice, antler, ginseng, Angelica, Cornus, Cordyceps, Yuryeongseon, Yeonhosaeng, Sewao, Hyangbuja, Samchil-Keun, Cheonmun-dong, Sat Fuling, Macmun-dong, Windproof, Wolfberry, Clan, Dog, Osuyu, Schisandra, Sansa, Sanchija, Sansuyu, Sanjoin, Sagunja, Jajagi, Casualties, Chason, Sohyanghyang, Red Soybeans, Changzai, Earthenware, Bokbunja, Mazain, Uiin, Eumseong , Dried grass, chajeoncho, sunhakcho, perilla leaf, breeding, non-leafed, pogongyoung, motherwort, yeonjeoncho, haegocho, Kanto, gold and silver, Seonhwa, Ogapi, phalanx, baekbaekpi, bark skin, sanggi, jogu etc. , Yellow flower, yellow lotus, grass roots, root, gil jing, jade bamboo, walrus, shiho, mountain, peony, peony, red ginseng, ginger, horse riding, cheongung, baekrye, rhubarb, taxa, dansam, cheonma, dangsam, venom, ginseng , Lily, white paper, rich, farting, windproof, cedar, ume, youngsil, passerby , Sancho, Jujube, Chitosan, Geumcho, Asparagine, Gallweed, Dermis, Guin, Malt, Chinese quince, Fedonia, Petal, Coniferous, Vitamin tree, Vitamin tree root, Vitamin tree branch, Vitamin tree leaf, Vitamin tree fruit, Mold, Fruit tree , Seshin, ephedra, chrysanthemum, safflower, god, clove, cinnamon, gourd, tofu, keg, ghost, ghost spirit, manor, new song, sea horse, pearl, island animal, civet, net, propolis, white Forming a primary mixing medium by mixing at least one or more of the herbal medicines 5 to 85 formed by mixing at least one (S30);

The sumac and lacquer bark and parts by weight herbal medicines can provide a medium that is mixed differently depending on the function.

4) mixing a nitrogen source material formed by mixing at least one or more from the first group consisting of casein, starch, soybean meal, bran, rice bran, brown rice powder to the primary mixing medium to form a secondary mixing medium by mixing 20 to 65 weight (S40);

5) secondary fermentation step (S50) inoculated with 0.5 to 2.5 parts by weight of the fermentation microorganisms in the secondary mixed medium and incubated for 3 to 20 days at a temperature of 15 to 45 ℃, humidity 55 to 85%;

The fermentation microorganisms Bacillus subtilis Bacillus subtilis and microbispora , Streptosporangium , Micromonospora , Nocardioforms , Dactilosporus It is a rare actinomycete from the group 1 consisting of Dactylosporangium , Saccharomonospora and Microtetraspora .

This strain is the intake of nutrients from a variety of sources of nitrogen in the natural world, mainly beta-lactam (β -lactam), macrolide (macrolide), amino glycosides, Avermectin-milbe azithromycin (avermectin-milbemycin) responsible for the synthesis of antibiotics of the type Do it.

6) a first sterilization step (S60) of sterilizing all contaminated strains including the inoculated strain by applying a temperature at 100 to 132 ° C. for 10 to 30 minutes using an autoclave to the fermentation product produced in the second fermentation step;

The sterilizing autocrab is preferably in principle to use a device that is automatically adjusted.

7) At least one or more from the first group consisting of a drying process for drying the first sterilized fermented product to a water content of 7% to 15% and casein, starch, soybean meal, bran, rice bran, brown rice, nitrogen and carbon sources Mixing the nitrogen source parts by weight 10 to 75 formed by mixing to form a tertiary mixing medium (S70);

8) the third fermentation step of inoculating 0.5 to 2.5 parts by weight of the fermentation microorganisms in the tertiary mixing medium and incubating for 2 to 30 days at a temperature of 15 to 58 ℃, humidity 50 to 85% (S80);

The fermentation microorganism used in the third fermentation step corresponds to the same as the fermentation microorganism used in the second fermentation step.

 9) a second sterilization step of sterilizing all contaminated strains including the inoculated strain by applying a temperature at 100 to 132 ° C. for 10 to 30 minutes using an autoclave to the third fermentation product produced in the third fermentation step (S90). );

10) Mixing and stirring an aqueous alcohol solution at a weight ratio of 40:60 to 50:50 to the fermentation product that has undergone the second sterilization step, and extracting the fermentation composition by extracting it under reduced pressure at a pressure of 1 to 10 Kgf / cm2 at a temperature of 40 to 60 ° C. Step (S100);

And the aqueous alcohol solution includes an extraction step (S100) is formed by distilled water 30 to 60 parts by weight, anhydrous ethanol parts by weight 40 to 70, and may further include a ripening step and the extracted fermentation composition temperature 10 to Aged at 15 ° C.

Including the third fermentation step as described above, as the fermentation is repeated, detoxify urushiol, which is a skin allergic substance contained in the oak tree and the bark of the lacquer, and make the molecular crust of the active ingredient small It is to be more effectively absorbed by the human body.

Hereinafter, the present invention will be described in detail through examples. The terms used are merely used for the purpose of describing the present invention and are not used to limit the scope of the present invention as defined in the meaning or claims.

[Example]

Shred and slice oak and oak bark, dilute soybean paste in distilled water 3-30% by weight, immerse it for 1-10 hours and soak it in a sieve to remove water for 1-2 hours, and then remove the dirt from the epidermis. After washing the impurities, apply 1 ~ 20cm of dried straw and chestnut leaves, lay 5 ~ 20cm of oak and oak bark, and pile up 1 ~ 20cm of rice straw and chestnut leaves repeatedly at 15 ~ 50 ℃. Ferment.

100 parts by weight of the first fermentation product and white berry, half, Angelica, licorice, deer antler, ginseng, Angelica, cornus, Cordyceps sinensis, yeongyeongseon, Yeonho color, Sewao, Hyangbuja, Samchil-geun, cheonmun-dong, Tobokyeong, Macmun-dong, windproof, Gugija , Ancestor, dog, sorghum, schisandra, mountain lion, gardener, wild squirrel, sanjoin, monsoon, jasper, casualty, chason, small fennel, red bean curd, changja, earthenware, bokbunja, mazain, uiyiin, yinyanggwa, lichen grass, chajeoncho, sunhakcho, Dried bamboo leaf, breeding, non-leafed, pogongyoung, motherwort, yeonjeoncho, haegocho, Kwandonghwa, gold silver flower, Seonhwa, Ogapi, phalanx, baekbaekpi, bark skin, sanggi, ancestors, gallant, turmeric, hwanggi, golden, hwangjeong, hwangryeon, original paper, Brown root, streetlight, jade bamboo, walrus, shiho, mountain, peony, peony, samsam, ginger, horseback riding, cheongung, baekrye, rhubarb, tax, dansam, cheonma, tangsam, poisonous ginseng, ginseng, falcon, half ginseng, lily, white paper, rich man, Bangui, Windproof, Mokhyang, Plum, Youngsil, Pedestrian, Sancho, Jujube, Chitosan, Gold Vinegar, asparagine, gallweed, dermis, mandarin, malt, quince, fragrance, leafy, locus, vitamin tree, vitamin tree root, vitamin tree branch, vitamin tree leaf, vitamin tree fruit, hedgehog, fern, sessin, ephedra, chrysanthemum, Safflower, god, clove, cinnamon, pepper, tofu, neck, ghost, ghostling, manor, singok, seahorse, pearl, island, shaggy musk, nets, propolis, is formed by mixing at least one or more from one group A primary mixing medium in which 5 to 85 parts by weight of the herbal medicine is mixed is formed.

Part by weight of the material formed by mixing at least one from the group consisting of casein, starch, soybean meal, bran, rice bran, brown rice powder consisting of carbon source and nitrogen source in the primary fermentation composition of the oak tree and the lacquer bark primary fermentation composition 20-65 are mixed to form a secondary mixing medium.

Bacillus subtilis and rare actinomycetes ( Rare) in the secondary mixed medium actinomycetes ) inoculated 0.5 to 2.5 parts by weight and incubated for 3 to 20 days at a temperature of 15 to 45 ℃, humidity 55 to 85%.

Known Bacillus subtilis and actinomycetes rare (Rare actinomycetes) If one also mubang to any use that, Bacillus Sup-blocks bus (Bacillus subtilis) and micro-bis Fora (Microbispora), streptomycin sports ditch declination (Streptosporangium), micro mono spokes La (Micromonospora), no carboxylic video form's (Nocardioforms ), Dactylosporangium , Saccharomonospora , Microtetraspora from the first group selected from the group of rare actinomycetes Bacillus subtilis (Bacillus subtilis) 0.5 to 2.5 It is preferable to inoculate.

The secondary fermentation product produced in the secondary fermentation step was sterilized all contaminated strains including the inoculated strains by autoclave temperature at 100-132 ° C. for 10-30 minutes and the moisture content was 7%. Dry to ˜15%.

Mixing the nitrogen source 10 parts by weight to 75 parts by weight of the fermented composition, which is formed by mixing at least one or more from one group consisting of nitrogen and carbon sources casein, starch, soybean meal, bran, rice bran, brown rice. In the secondary mixed medium Bacillus Sup-blocks bus (Bacillus subtilis), micro-bis Fora (Microbispora), streptomycin sports ditch declination (Streptosporangium), micro mono spokes La (Micromonospora), no carboxylic video form's (Nocardioforms), spokes with daktil Bacillus subtilis and rare actinomycetes among Dactylosporangium , Saccharomonospora , and Microtetraspora were inoculated at least one group to inoculate 0.5 to 2.5 parts by weight, and the temperature was 15. Fermentation for 3 to 20 days at 45 to 45 ℃, humidity 55 to 85%, the third fermentation product produced in the third fermentation step by using the autoclaver at 100 ~ 132 ℃ temperature for 10 to 30 minutes and other inoculated strains Sterilize all strains and dry the fermentation product so that the moisture content is 7% to 15%.

The aqueous solution of alcohol is mixed with the dried tertiary fermentation product, stirred, and extracted under reduced pressure at a pressure of 1 to 10 Kgf / cm 2 at a temperature of 40 to 60 ° C. to extract the fermentation composition. The aqueous alcohol solution is formed from 30 to 60 parts by weight of distilled water, 40 to 70 parts by weight of anhydrous ethanol, and includes an extraction step of extracting, and may further include a aging step.

The extracted oak and oak bark fermentation compositions are aged at a temperature of 10 to 15 ° C.

So far, the present invention has been described with reference to the embodiments, which are merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent other embodiments are possible. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

S10 ~ S10: manufacturing step of fermentation composition

Claims (5)

Crushing and slicing lacquer and lacquer bark, dipping soybean paste in distilled water by diluting 3-30% by weight, immersing in soaked for 1-10 hours and soaking, and then removing lacquer and lacquer medium for 1-2 hours. ;
1-20 cm of rice straw and chestnut leaves, and then 5-5 cm of lacquer and lacquer bark medium, and piled 1-20 cm of rice straw and chestnut leaves by primary fermentation at 15-50 ° C .;
100 parts by weight of the fermentation products produced in the first fermentation step and white berry, half, donkey, licorice, antler, ginseng, tangui, cornus, cordyceps, yeongseonseon, Yeonho color, sewage, hyangbuja, samchieun, cheonmundong, Tobokyeong, Magmundong, Windproof, Wolfberry, Catch, Cattle, Filthyra, Schisandra, Sansa, Sanchija, Sansuyu, Sanjoin, Sagunja, Jajagi, Casualties, Chason, Sohyanghyang, Red Soybean, Changja, Sasa, Bokbunja, Mazarine, Uiin, Eumseong , Chajeoncho, Seonhakcho, Daffodil, Breeding, Non-foliage, Pogongyoung, Motherwort, Yeonjeoncho, Hagocho, Kanto, Gold, Silver, Bokhwa, Ogapi, Jigolpi, Sangbaekpi, Barkpi, Sanggi, Joguung, Mungja, Turmeric, Hwanggi, Golden, Hwangjeong , Yellow lotus, grass, root, street, jade bamboo, walnut, sea lake, mountain chemical, yellow flower, peony, four seasons, ginger, horseback riding, celestial organ, baekrye, rhubarb, taxa, dansam, cheonma, dangsam, poisonous ginseng, ginseng , White paper, rich man, farting, windproof, cedar, plum, youngsil, passerby, Vinegar, jujube, chitosan, edible vinegar, asparagine, gallweed, dermis, cauliflower, malt, quince, pontoon, leafy, eccentricity, vitamin tree, vitamin tree root, vitamin tree branch, vitamin tree leaf, vitamin tree fruit, mold, hut, Seishin, ephedra, chrysanthemum, safflower, god, clove, cinnamon, thick, tofu, neck, ghost, ghost, manor, new song, seahorse, pearl, sea animal, civet, net, propolis, white Mixing the at least one herbal medicine part 5 to 85 formed by mixing one or more to form a primary mixing medium;
Mixing a nitrogen source material formed by mixing at least one or more from one group consisting of casein, starch, soybean meal, bran, rice bran, and brown rice powder to the primary mixing medium to form a secondary mixing medium by mixing 20 to 65 weights;
Fermenting microorganisms by weight inoculating 0.5 to 2.5 parts by weight of the secondary mixed medium and a secondary fermentation step of incubation for 3 to 20 days at a temperature of 15 to 45 ℃, humidity 55 to 85%;
A first sterilization step of sterilizing all contaminated strains including the inoculated strain by applying a temperature at 100 to 132 ° C. for 10 to 30 minutes using an autoclave to the fermentation product produced in the second fermentation step;
The first sterilized fermentation product is dried at a moisture content of 7% to 15% and a mixture of at least one from the group consisting of nitrogen and carbon sources casein, starch, soybean meal, bran, rice bran, brown rice flour Mixing the formed nitrogen source 10 to 75 parts to form a tertiary mixing medium;
A third fermentation step of inoculating 0.5 to 2.5 parts by weight of the fermentation microorganisms in the tertiary mixing medium and incubating for 2 to 30 days at a temperature of 15 to 58 ° C. and a humidity of 50 to 85%;
A second sterilization step of sterilizing all contaminated strains including the inoculated strain by applying a temperature at 100 to 132 ° C. for 10 to 30 minutes using an autoclave to the third fermentation product produced in the third fermentation step;
Mixing and stirring an aqueous solution of alcohol in a fermentation product passed through the second sterilization at a weight ratio of 40: 60 to 50: 50, stirring to extract the reduced pressure at a pressure of 1 to 10 Kgf / ㎠ at a temperature of 40 to 60 ℃; Fermentation composition production method using lacquer and lacquer bark.
The method of claim 1,
The fermenting microorganism is Bacillus Sup-blocks bus (Bacillus subtilis) and micro-bis Fora (Microbispora), streptomycin sports ditch declination (Streptosporangium), micro mono spokes La (Micromonospora), no carboxylic video form's (Nocardioforms), Spokane ditch declination in daktil ( Dactylosporangium ), Saccharomonospora ( Saccharomonospora ), Microtetraspora ( Microtetraspora ), characterized in that the first group of lacquer and lacquer bark production method using the lacquer bark.
The method of claim 1,
The aqueous alcohol solution is a method for producing a fermentation composition using lacquer and lacquer, characterized in that the distilled water 30 to 60 parts by weight, anhydrous ethanol parts by weight 40 to 70.
The method of claim 1,
Method for producing a fermentation composition using lacquer and lacquer bark, characterized in that it further comprises the step of ripening the extracted fermentation composition at a temperature of 10 to 15 ℃.
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