CN113930295A

CN113930295A - Cleaning composition containing biological enzyme and preparation method thereof

Info

Publication number: CN113930295A
Application number: CN202111324958.2A
Authority: CN
Inventors: 程绍禹; 叶自溢
Original assignee: Guangzhou Aijia Youfang Daily Necessities Co ltd
Current assignee: Guangzhou Aijia Youfang Daily Necessities Co ltd
Priority date: 2021-11-10
Filing date: 2021-11-10
Publication date: 2022-01-14

Abstract

The invention provides a cleaning composition containing biological enzyme and a preparation method thereof, relates to the technical field of biological enzyme, and provides a ferment composition which comprises amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme. The cleaning composition can be obtained by compounding the cleaning composition with components such as solvent, surfactant, filler and bactericide, the raw materials of the cleaning composition are natural, safe, nontoxic and nonirritating, and the edible cleaning composition provided by combining the active ingredients of the surfactant, enzyme composition and bactericide realizes excellent decontamination and bacteriostasis performance, and simultaneously, the dosage of the surfactant is obviously reduced.

Description

Cleaning composition containing biological enzyme and preparation method thereof

Technical Field

The invention relates to the technical field of biological enzyme, and particularly relates to a cleaning composition containing biological enzyme and a preparation method thereof.

Background

The cleaning agent is a general name of cleaning products used for washing clothes, cleaning tools, cleaning furniture and the like, and becomes an indispensable daily chemical product in daily life. In order to ensure good cleaning effect, the cleaning agent used in daily life usually contains inedible components, the content of the surfactant is high, part of chemical components contained in the cleaning agent are easy to generate irritation to human skin and the like, the biodegradation degree of the cleaning agent is low, and environmental pollution is easy to cause. The safety of the existing daily chemical cleaning agent is not ideal enough, and if people or animals eat the cleaning agent by mistake, the health of the people or animals can be affected, and even the personal safety is harmed.

The biosurfactant is a metabolite with certain surface activity secreted by microorganisms in a metabolic process under certain conditions, such as glycolipid, polysaccharide lipid, lipopeptide or neutral lipid derivative. Although the biosurfactant product has the advantages of good performance, safety, environmental protection and easy degradation, the industrial production of the biosurfactant product is still in the initial stage, the production cost and the application cost are higher, the global dosage of the biosurfactant is less than 2 percent of the global dosage of all the biosurfactants, and the industrial application of the biosurfactant product in the daily chemical field is limited to a certain extent.

For example, patent No. cn201811198188.x discloses a cleaning composition, its application and a cleaning agent, the composition contains a chelating agent, a polymer containing polyoxyethylene ether chain segment, a defoaming agent, a nonionic surfactant and an anionic surfactant, and further contains a complex enzyme, the complex enzyme contains at least two enzymes of protease, lipase, amylase and cellulase. However, the content of the surfactant in the invention is high, which reaches more than 15 percent, even more than 30 percent, and the safety and environmental protection are still to be studied. And patent CN201810039671.7 discloses a biodegradable environment-friendly glass cleaning solution, which comprises: 3-8 parts of biological surfactant, 0.1-0.5 part of biological enzyme compound agent, 1-5 parts of biological corrosion inhibitor, 50-80 parts of water and 10-40 parts of freezing point depressant, wherein the biological enzyme compound agent contains at least one of protease, lipase, amylase, cellulase, pectinase, catalase and lysozyme, and the cleaning solution can completely realize biodegradation, has zero pollution to the environment, is almost harmless to human, animals and plants and the like, and is safe and environment-friendly. However, the invention does not further examine the safety, bacteriostasis and oil stain removal capability of the related cleaning solution.

Therefore, aiming at the problems of high surfactant content, poor safety and environmental protection and the like of related cleaning products in the prior art, the search for a cleaning composition with low surfactant content, safety and environmental protection is of great significance.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a cleaning composition containing biological enzyme and a preparation method thereof, the raw materials of the composition are natural, safe, nontoxic and nonirritating, and the edible cleaning composition provided by the combination of active ingredients of a surfactant, an enzyme composition and a bactericide realizes excellent decontamination and bacteriostasis performance and simultaneously obviously reduces the dosage of the surfactant.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the invention provides an enzyme composition, which consists of amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme.

Further, the weight ratio of the amylase, the protease, the cellulase, the lipase, the superoxide dismutase, the pectinase and the lysozyme is 0.5-2:1-3:1-5:0.5-2:0.1-1:0.1-1: 0.1-1. Preferably in a weight ratio of 1:2:4:1:0.5:0.5: 0.5.

The invention also provides a composition containing the biological enzyme, which comprises the following raw materials: solvent, surfactant, filler, bactericide and the enzyme composition.

Further, the composition comprises the following raw materials in parts by weight: 10-50 parts of solvent, 2-15 parts of surfactant, 20-50 parts of filler, 0.1-5 parts of bactericide and 0.5-5 parts of enzyme composition.

Preferably, the composition comprises the following raw materials in parts by weight: 30-50 parts of solvent, 5-10 parts of surfactant, 25-40 parts of filler, 0.2-4 parts of bactericide and 1-3 parts of enzyme composition.

Further preferably, the composition comprises the following raw materials in parts by weight: 48 parts of solvent, 8 parts of surfactant, 30 parts of filler, 1 part of bactericide and 2 parts of the enzyme composition.

Further, the weight ratio of the surfactant to the enzyme composition is 2-20:0.5-5, preferably 5-10:1-3, and more preferably 4: 1.

Further, the surfactant includes a biological surfactant. Preferably, the surfactant comprises one or more of rhamnolipid, sophorolipid, algal glycolipid, phospholipid, neutral lipid and SD 100.

Further, the solvent comprises one or more of ethanol, propylene glycol, glycerol and water; the bactericide comprises one or more of eucalyptus essential oil, tea tree essential oil, lemon essential oil and mint essential oil.

Further, the filler comprises one or more of glucose, sodium gluconate, xylitol, maltose, lactose and pregelatinized starch.

The invention also provides a preparation method of the composition, which is characterized by comprising the following steps: the method comprises the following steps:

(1) mixing a solvent and a filler, homogenizing and shearing to obtain a first mixture;

(2) adding a surfactant and an enzyme composition into the first mixture, homogenizing and shearing to obtain a second mixture;

(3) adding bactericide into the second mixture, homogenizing, shearing, and stirring to obtain composition containing biological enzyme.

Further, the time of the homogenizing shearing in the step (1) is 20-50min, the time of the homogenizing shearing in the step (2) is 10-30min, and the time of the homogenizing shearing in the step (3) is 5-20 min.

The composition of the present invention can be applied to the cleaning of clothes, the cleaning of kitchen ware, the cleaning of tableware and the cleaning of toys.

The technical effects obtained by the invention are as follows:

the raw materials used in the invention are natural, safe, nontoxic and nonirritating, the edible cleaning composition provided by the invention realizes excellent decontamination and bacteriostasis performance by combining the active ingredients of the surfactant, the enzyme composition and the bactericide, the dosage of the surfactant is obviously reduced (the dosage of the surfactant in related products sold in the market is usually more than 13%), the provided cleaning composition is safe, edible and stable in performance, the synergistic effect between the surfactant and the biological enzyme in the invention realizes the consideration of decontamination, bacteriostasis and safety performance, the harm caused by the mistaken eating of children and pets can be effectively avoided, and the edible cleaning composition can be widely applied to cleaning clothes, tableware, toys and the like. The preparation method of the edible cleaning composition provided by the invention is simple, and the prepared edible cleaning composition has stable quality. Furthermore, the present invention provides the use of an edible cleaning composition for cleaning laundry, cleaning kitchen ware, cleaning dishware and cleaning toys.

Detailed Description

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.

Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.

When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

It is to be noted that the raw materials used in the present invention are all common commercial products, and can be obtained by means of conventional techniques in the art. For example: rhamnolipid is purchased from Shanghai Yingchuang chemical Co., Ltd, sophorolipid is purchased from Shanghai Yingchuang chemical Co., Ltd, cellulase S is purchased from Novoxin Biotech Co., Youjiali essential oil is purchased from Jiangxi Zhongxiang perfume Co., Ltd, tea tree essential oil is purchased from Jiangxi Zhongxiang perfume Co., Ltd, and SD100 is TEGO TENS SD 100.

Example 1

A composition containing biological enzyme comprises the following raw materials in parts by weight: 10 parts of solvent (specifically 7 parts of propylene glycol, 1 part of ethanol and 2 parts of glycerol), 2 parts of surfactant (specifically 1001 parts of SD and 1 part of sophorolipid), 20 parts of filler (specifically 10 parts of glucose and 10 parts of maltodextrin), 0.1 part of eucalyptus essential oil and 0.5 part of enzyme composition. The enzyme composition comprises amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme in a weight ratio of 0.5:1:1:0.5:0.1:0.1: 0.1.

The preparation method of the composition containing the biological enzyme comprises the following steps:

(1) mixing a solvent and a filler, and homogenizing and shearing for 20min to obtain a first mixture;

(2) adding a surfactant and an enzyme composition into the first mixture, and homogenizing and shearing for 10min to obtain a second mixture;

(3) adding eucalyptus essential oil into the second mixture, homogenizing and shearing for 5min, and stirring to obtain composition containing biological enzyme.

Example 2

A composition containing biological enzyme comprises the following raw materials in parts by weight: 50 parts of solvent (specifically 30 parts of propylene glycol, 10 parts of ethanol and 10 parts of glycerol), 15 parts of surfactant (specifically 10 parts of rhamnolipid and 5 parts of algal glycolipid), 50 parts of filler (specifically 25 parts of sodium gluconate and 25 parts of maltodextrin), 5 parts of tea tree essential oil and 5 parts of enzyme composition. The enzyme composition comprises amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme in a weight ratio of 2:3:5:2:1:1: 1.

(1) mixing a solvent and a filler, homogenizing and shearing for 50min to obtain a first mixture;

(2) adding a surfactant and an enzyme composition into the first mixture, and homogenizing and shearing for 30min to obtain a second mixture;

(3) adding tea tree essential oil into the second mixture, homogenizing and shearing for 20min, and stirring to obtain composition containing biological enzyme.

Example 3

A composition containing biological enzyme comprises the following raw materials in parts by weight: 48 parts of solvent (30 parts of propylene glycol, 10 parts of ethanol and 8 parts of glycerol), 8 parts of surfactant (5 parts of trehalose glycolipid and 3 parts of phospholipid), 30 parts of filler (20 parts of glucose and 10 parts of maltodextrin), 1 part of lemon essential oil and 2 parts of enzyme composition. The enzyme composition comprises amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme in a weight ratio of 1:2:4:1:0.5:0.5: 0.5.

(1) mixing a solvent and a filler, homogenizing and shearing for 40min to obtain a first mixture;

(2) adding a surfactant and an enzyme composition into the first mixture, and homogenizing and shearing for 20min to obtain a second mixture;

(3) adding lemon essential oil into the second mixture, homogenizing and shearing for 10min, and stirring to obtain composition containing biological enzyme.

Example 4

A composition containing biological enzyme comprises the following raw materials in parts by weight: 30 parts of solvent (specifically 20 parts of ethanol and 10 parts of glycerol), 5 parts of surfactant (specifically 3 parts of trehalose and glycolipid and 2 parts of neutral fat), 25 parts of filler (specifically 20 parts of glucose and 5 parts of maltodextrin), 0.2 part of mint essential oil and 1 part of the enzyme composition. The enzyme composition comprises amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme in a weight ratio of 0.5:3:1:2:0.1:1: 0.1.

(3) adding the peppermint essential oil into the second mixture, homogenizing and shearing for 5min, and stirring uniformly to obtain the composition containing the biological enzyme.

Example 5

A composition containing biological enzyme comprises the following raw materials in parts by weight: 50 parts of solvent (specifically 20 parts of propylene glycol, 10 parts of glycerol and 20 parts of water), 10 parts of surfactant (specifically 5 parts of sophorolipid, 3 parts of phospholipid and 2 parts of neutral fat), 40 parts of filler (specifically 25 parts of xylitol, 10 parts of lactose and 5 parts of pregelatinized starch), 4 parts of eucalyptus essential oil and 3 parts of the enzyme composition. The enzyme composition comprises amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme in a weight ratio of 2:1:5:0.5:1:0.1: 1.

(3) adding eucalyptus essential oil into the second mixture, homogenizing and shearing for 20min, and stirring to obtain composition containing biological enzyme.

Comparative example 1

The only difference from example 1 is that the enzyme composition is amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme in a weight ratio of 3:0.5:6:0.3:1.5:0.05:1.5 (the total weight of seven being identical to example 1).

Comparative example 2

The only difference from example 1 was that the superoxide dismutase in the enzyme composition was replaced with an equal amount of catalase.

Comparative example 3

The only difference from example 1 is that the weight ratio of surfactant to ferment composition is 1:7 (the total weight of both is identical to example 1).

Safety test

The test method comprises the following steps: toxicology tests are carried out according to GB15193.1-2014 'food safety toxicology evaluation program', a maximum tolerance metering test method is adopted, the dosage is set as 15000mg/kg BW, 80 Kunming mice are selected and randomly divided into 8 groups, each group comprises 10 mice, and each half of the mice are male and female. Animals were fasted for 16h before the test, without any restriction on water. The animals were administered 800mg/mL samples of each test substance using a gavage method, with a gavage amount of 0.2mL/10g body weight. Observing the behavior of the mice after gastric lavage, weighing 1 time per week, observing 14d, and calculating LD₅₀The results are tabulated in table 1.

The test substance: in each example, 8 samples of the composition containing the biological enzyme were used in 8 groups.

Table 1 safety test results for compositions of the invention

Examples of the invention	LD₅₀(mg/kg.BW)
		Example 1	28160
Example 2	28200
		Example 3	28750
Example 4	28230
		Example 5	28500
Comparative example 1	25750
		Comparative example 2	27080
Comparative example 3	25500

LD of propylene glycol as the main raw material added in the present invention₅₀22000mg/kg (oral administration in mice), while the LD of the composition of each example in the present invention₅₀More than 28000mg/kg, thus indicating that the product of the invention is safe under the premise of specific raw material proportion.

Second, bacteriostatic test

The test method comprises the following steps: carrying out bacteriostatic performance test according to the method in GB 9985 + 2000 detergent for washing tableware by hand, and counting the results to table 2;

the test substance: the compositions containing the biological enzymes and the commercial detergents in each example were diluted 10-fold and tested, for 9 samples in 9 groups.

Table 2 bacteriostatic test results of the compositions of the present invention

The above table shows that the composition has good antibacterial effect, the antibacterial rate of the diluted composition on staphylococcus aureus reaches more than 98.2%, the antibacterial rate on candida albicans reaches more than 97.3%, the antibacterial rate on escherichia coli reaches more than 98.2%, and the composition is obviously superior to the detergent sold in the market.

Third, oil stain removal performance test on hard object surface

The test method comprises the following steps: the surface of the model hard object was a 10cm × 10cm glass plate, and before use, all the glass plates were washed with chloroform and sufficiently dried, and the initial mass (m) was recorded₀) About 0.1g of oil stain (to the nearest 0.0001g) was applied and used after aging in an oven at 60 ℃ overnight. Drying at room temperature for 4h, recording the weight as m₁. Washing treatment: in a decontamination machine, 9 test articles (product in each example of the invention and commercial detergent 98.1) were prepared, one set of wash water corresponding to one set of glass plates was soaked in wash water at 30 ℃ for 1min and washed for 2.5min, then the dishes were taken out and dried for 4h, the recorded weight m₂. The average stain removal per group was calculated: the removal efficiency of the oil stain was calculated by the following formula, and the results are shown in Table 3.

The test substance: tap water containing 0.1% of the composition containing the biological enzyme in each example, 8 samples in 8 groups;

TABLE 3 greasy dirt removal performance results for the compositions of the invention

Examples of the invention	Oil removal Rate (%)
		Example 1	99.0
Examples2	98.7
		Example 3	99.6
Example 4	99.0
		Example 5	99.5
Comparative example 1	94.8
		Comparative example 2	97.5
Comparative example 3	82.5
		Commercial detergent	98.1

The above table shows that the composition of the invention has good oil removal effect, the overall oil removal rate reaches more than 98.7%, and the composition is obviously superior to the commercial detergent.

Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. An enzyme composition, comprising: consists of amylase, protease, cellulase, lipase, superoxide dismutase, pectinase and lysozyme.

2. The ferment composition of claim 1, wherein: the weight ratio of the amylase to the protease to the cellulase to the lipase to the superoxide dismutase to the pectinase to the lysozyme is 0.5-2:1-3:1-5:0.5-2:0.1-1:0.1-1: 0.1-1.

3. A composition comprising a biological enzyme, wherein: the method comprises the following raw materials: a solvent, a surfactant, a filler, a bactericide and the enzyme composition of claim 1.

4. The composition of claim 3, wherein: the composite material comprises the following raw materials in parts by weight: 10-50 parts of solvent, 2-15 parts of surfactant, 20-50 parts of filler, 0.1-5 parts of bactericide and 0.5-5 parts of enzyme composition as claimed in claim 1.

5. The composition of claim 4, wherein: the composite material comprises the following raw materials in parts by weight: 30-50 parts of solvent, 5-10 parts of surfactant, 25-40 parts of filler, 0.2-4 parts of bactericide and 1-3 parts of enzyme composition as claimed in claim 1.

6. The composition of claim 3, wherein: the surfactant comprises a biological surfactant.

7. The composition of claim 6, wherein: the surfactant comprises one or more of rhamnolipid, sophorolipid, algal glycolipid, phospholipid, neutral lipid and SD 100.

8. The composition of claim 3, wherein: the solvent comprises one or more of ethanol, propylene glycol, glycerol and water; the bactericide comprises one or more of eucalyptus essential oil, tea tree essential oil, lemon essential oil and mint essential oil.

9. A process for preparing a composition according to any one of claims 3 to 8, characterized in that: the method comprises the following steps:

10. Use of a composition according to any one of claims 3 to 8 for cleaning laundry, cleaning kitchen ware, cleaning dishware and cleaning toys.